RT-PCR & cDNA Synthesis Products

One-Step RT-PCR Kits

<h2 dir="ltr">What Are One-Step RT-PCR Kits?</h2>
 A one-step RT-PCR kit combines reverse transcription and PCR amplification in a single reaction tube and buffer, using gene-specific primers throughout. The kit's enzyme mix typically pairs a reverse transcriptase -- commonly an M-MuLV-derived enzyme such as ProtoScript II or PrimeScript RT -- with a hot-start Taq DNA polymerase, sometimes including a proofreading component for higher-yield amplification.&nbsp; Choose a one-step kit for routine, single- or few-target RNA detection where simplicity and contamination control matter most; choose a two-step approach instead when one RNA sample needs to be tested against many different gene targets.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li style="font-family: arial, helvetica, sans-serif; color: #000000;">One-step RT-PCR kits combining reverse transcription and PCR amplification in a single reaction workflow, including&nbsp;Azura 1-Step Ultra RT-PCR Kit</li>
<li style="color: #000000;">Standard one-step RT-PCR systems for RNA detection and target amplification, including One-Step RT-PCR Kit (RT0201-00) and Accuris One-Step RT-PCR Kit</li>
<li style="color: #000000;">Multiplex and probe-based one-step RT-qPCR-compatible systems, including Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit (No ROX)</li>
<li style="color: #000000;">High-performance one-step RT-PCR chemistry designed for robust RNA amplification, including the MegaFi One-Step RT-PCR Kit</li>
<li style="color: #000000;">Ready-to-use enzyme and buffer systems optimized for streamlined RT-PCR setup and reproducible results across RNA workflows</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-PCR Kit</h2>
 Enzyme Mix Composition Kits commonly combine an M-MuLV-derived reverse transcriptase with reduced RNase H activity and increased thermostability (e.g., ProtoScript II-type or PrimeScript-type enzymes) with a hot-start Taq polymerase. Some formulations add a proofreading polymerase component for higher-yield, more accurate end-point amplification. Hot-Start Activation Mechanism Most one-step kits use an antibody-modified or aptamer-based hot-start Taq that remains inactive at room temperature, dissociating during an initial high-temperature step (commonly around 94-95 &deg;C for several minutes) to simultaneously activate the polymerase and inactivate the reverse transcriptase before PCR cycling begins. Template Difficulty and Amplicon Length Reverse transcriptases with reduced RNase H activity and enhanced thermostability are better suited to GC-rich or highly structured RNA templates. Some one-step kits are validated for amplifying long transcripts -- up to approximately 9 kb in certain formulations -- relevant for full-length transcript or large-gene RT-PCR. Single-Tube Enzyme Compatibility Combining reverse transcriptase and Taq polymerase in one buffer historically risked mutual inhibition between the two enzyme classes; modern one-step formulations use optimised buffer systems and enzyme ratios specifically designed to avoid this interference, a key differentiator between basic and well-optimised kits. Detection Format End-point one-step RT-PCR kits (gel-based detection) suit qualitative presence/absence screening. For quantitative results, a one-step RT-qPCR kit with a fluorescent dye or probe system is the appropriate choice instead. 
<h2 dir="ltr"> Specifications Context</h2>
 The one-step format integrates the three RT-PCR steps -- reverse transcription, polymerase activation, and amplification -- into a single continuous reaction without opening the tube between steps, directly reducing contamination risk relative to workflows that transfer reaction products between vessels. Historically, combining reverse transcriptase and Taq polymerase in a shared buffer was reported to cause mutual enzyme inhibition and reduced sensitivity; current one-step kits address this through optimised buffer chemistry and enzyme selection, such as RNase H-minus reverse transcriptases paired with hot-start Taq formulations. One-step kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light, where fluorescent dyes are pre-included; lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-precision One-Step RT-PCR Kits for your lab.

User Manual

Azura 1-Step Ultra RT-PCR Kit - Product Sheet

MSDS

Azura 1-Step Ultra RT-PCR Kit

AZ-1825 by Azura Genomics — single-tube 1-step RT-PCR kit; 1 pg–1 µg total RNA input; amplicons up to 3 kb; 2x HS Taq mix + 20x thermostable RT blend; ISO 9001:2015; 50 reactions.

Manufacturer

Azura 1-Step Ultra RT-PCR Kit 50 Reactions

Azura 1-Step Ultra RT-PCR Kit 100 Reactions

cDNA Synthesis Kits

<h2 dir="ltr"> What is a cDNA Synthesis Kit?</h2>
 A cDNA synthesis kit provides the complete set of reagents - reverse transcriptase enzyme, reaction buffer, dNTPs, RNase inhibitor, and primers - needed to perform first-strand reverse transcription, converting an RNA sample into a cDNA pool. Unlike one-step RT-PCR kits, which combine RT and PCR in a single reaction with gene-specific primers, a cDNA synthesis kit performs reverse transcription as a standalone step, producing a cDNA template that can be used across multiple subsequent PCR or qPCR reactions targeting different genes. Choose a cDNA synthesis kit when one RNA sample needs to be analysed for multiple gene targets, or when downstream qPCR requires a separately-generated, quality-checked cDNA template. 
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="1o7fvd2" data-start="74" data-end="228">First-strand cDNA synthesis kits for RNA-to-cDNA conversion in gene expression workflows, including AzuraQuant&trade; and AzuraFlex&trade; cDNA synthesis kits</li>
<li data-section-id="z40sk0" data-start="229" data-end="395">Standard cDNA synthesis kits for routine transcriptional analysis and downstream PCR applications, including Accuris qMAX and 1st cDNA Synthesis Kit (FS01-00)</li>
<li data-section-id="1yvybqf" data-start="396" data-end="521">Flexible and high-efficiency cDNA synthesis systems designed to support a range of RNA inputs and experimental conditions</li>
<li data-section-id="sl2teb" data-start="522" data-end="599">miRNA-specific cDNA synthesis solutions for small RNA expression analysis</li>
<li data-section-id="17gz9kt" data-start="600" data-end="770" data-is-last-node="">One-step and integrated RT-based systems and master mixes supporting streamlined reverse transcription workflows, including OneScript Plus and OneScript Hot RT kits</li>
</ul>
<h2 dir="ltr"> How to Choose a cDNA Synthesis Kit</h2>
 Priming Strategy Oligo(dT) primers target the poly-A tail of mRNA, enriching specifically for mRNA-derived cDNA and reducing genomic DNA or rRNA background. Random primers (commonly hexamers) prime throughout the RNA template regardless of sequence, useful for templates lacking a poly-A tail (such as bacterial RNA) or when full-length cDNA coverage, including 5' regions, is needed. Some kits offer a blend of both primer types for balanced coverage. Reverse Transcriptase Included Kits built around M-MLV RNase H-minus reverse transcriptase favour longer, more complete cDNA products, while kits using enhanced-thermostability or inhibitor-resistant reverse transcriptases may better handle GC-rich templates or minimally purified RNA samples. Genomic DNA Elimination Step Some cDNA synthesis kits include a separate genomic DNA elimination (gDNA wipeout) step performed before reverse transcription -- important for accurate gene expression quantification, since residual genomic DNA can contribute false signal in downstream qPCR if primers span regions present in both genomic DNA and the cDNA target. Reaction Volume and Throughput cDNA synthesis kits are available in formats scaled for individual reactions through to high-throughput 96-well formats, allowing labs to match kit format to typical sample batch sizes without excessive reagent waste. Downstream Application Compatibility The resulting cDNA pool should be compatible with the intended downstream application -- standard PCR, qPCR with SYBR Green or probe-based detection, or digital PCR -- confirm that the kit's buffer system does not interfere with the chosen downstream detection chemistry. 
<h2 dir="ltr"> Specifications Context</h2>
 In a two-step workflow, the first step synthesises first-strand cDNA using a reverse transcriptase such as M-MLV-RT with the chosen priming strategy, and the resulting cDNA pool can then be used in multiple separate PCR or qPCR reactions targeting different genes - a key advantage when a single RNA sample needs to be analysed for several targets, since the cDNA synthesis step is performed only once. Genomic DNA elimination steps, where included, are typically performed as a brief pre-treatment with a DNase before the reverse transcription reaction itself begins. cDNA synthesis kit components - enzyme, buffer, dNTPs, and RNase inhibitor - are typically stored at &minus;20 &deg;C; RNase-free water and RNase inhibitor handling throughout the workflow are recommended to prevent RNA template degradation before synthesis.
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-precision cDNA synthesis kits for your lab.

Product Literature

AzuraFlex™ cDNA Synthesis Kit

AZ-1997 AzuraFlex cDNA Synthesis Kit by Azura Genomics converts 10 pg–2 µg total RNA into cDNA (up to 9 kb) using MMLV-derived reverse transcriptase with random hexamer and oligo(dT) primers. 20 µl reactions; −20°C storage.

AzuraFlex cDNA Synthesis Kit 200 Reactions

AzuraFlex cDNA Synthesis Kit 1000 Reactions

AzuraQuant™ II cDNA Synthesis Kit

AZ-2501 by Azura Genomics is a 2-tube first-strand cDNA synthesis kit built on HighTherm™ II reverse transcriptase. Converts 4 pg to 2 µg total RNA to cDNA in 25 minutes using a combined random hexamer and anchored oligo(dT) strategy. Available in 25, 100, and 500 reaction formats for 2-step RT-qPCR and RT-PCR workflows.

AzuraQuant II cDNA Synthesis Kit 25 Reactions

AzuraQuant II cDNA Synthesis Kit 100 Reactions

AzuraQuant II cDNA Synthesis Kit 500 Reactions

AzuraQuant cDNA Synthesis Kit - Product Sheet

AzuraQuant™ cDNA Synthesis Kit

AZ-1995 by Azura Genomics is the AzuraQuant™ cDNA Synthesis Kit for 2-step RT-qPCR and RT-PCR. Two-tube format: 5x Buffer and 20x HighTherm™ RT with RNase inhibitor, plus random hexamer and anchored oligo(dT) primers. RNA input from 4 pg. Available in 25, 100, and 500 reaction sizes.

AzuraQuant cDNA Synthesis Kit 25 Reactions

AzuraQuant cDNA Synthesis Kit 500 Reactions

AzuraQuant cDNA Synthesis Kit 100 reactions

Reverse Transcriptase

<h2>What Is Reverse Transcriptase?</h2>
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Reverse transcriptase is an RNA-dependent DNA polymerase that synthesizes a complementary DNA (cDNA) strand using an RNA template, providing the essential first step for RT-PCR, RT-qPCR, cDNA library construction, and gene expression analysis. Reverse transcriptases differ in thermostability, processivity, RNase H activity, and compatibility with challenging RNA templates. Selecting the appropriate enzyme depends on your RNA quality, transcript length, and downstream application.
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<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Reverse transcriptase-based systems designed to support cDNA synthesis across a range of RNA templates, including workflows involving structured or challenging RNA.</li>
<li>One-step RT-PCR and RT-qPCR kits featuring enzyme systems designed to reduce non-specific amplification through optimized reaction setup and enzyme activation strategies.</li>
<li>High-efficiency reverse transcription and RT-PCR chemistries suitable for low-abundance RNA inputs and sensitive gene expression analysis.</li>
<li>cDNA synthesis and RT-PCR systems supporting a range of transcript lengths depending on enzyme selection and reaction conditions.</li>
<li>Thermostable and high-performance reverse transcription systems designed to improve cDNA yield and workflow reliability across RNA applications.</li>
</ul>
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<h2>How to Choose a Reverse Transcriptase</h2>
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Enzyme Selection
Reverse transcriptases differ in thermostability, RNase H activity, processivity, and compatibility with different RNA templates. Select an enzyme that matches your RNA quality, transcript length, and downstream application requirements.
RNase H Activity
Reverse transcriptases with reduced RNase H activity minimize RNA template degradation during cDNA synthesis, helping improve the yield of longer cDNA products for applications such as full-length transcript analysis and cDNA library preparation.
Thermostability
Thermostable reverse transcriptases can operate at higher reaction temperatures, helping reduce RNA secondary structure and improving cDNA synthesis from GC-rich or highly structured RNA templates.
Template Length and Application
Choose an enzyme validated for your intended transcript length and downstream workflow, whether performing routine RT-PCR, quantitative RT-qPCR, full-length cDNA synthesis, or library preparation.
Workflow Compatibility
Consider whether a stand-alone reverse transcriptase or an integrated one-step RT-PCR system best fits your workflow, throughput requirements, and downstream amplification strategy.
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<h2>Specifications Context</h2>
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Reverse transcriptase enzymes catalyze the synthesis of complementary DNA (cDNA) from RNA templates, enabling downstream PCR- and qPCR-based analysis of gene expression. Modern reverse transcriptases are available with varying levels of thermostability, processivity, and RNase H activity to support applications ranging from routine first-strand cDNA synthesis to amplification of structured or low-abundance RNA targets. Most enzymes are supplied for storage at &minus;20&nbsp;&deg;C, and RNase-free handling practices are recommended throughout RNA workflows to preserve sample integrity and maximize cDNA yield.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get research-grade reverse transcription enzymes for your lab.

HighTherm Reverse Transcriptase - Product Sheet

HighTherm™ Reverse Transcriptase

AZ-1991 by Azura Genomics: HighTherm™ Reverse Transcriptase — engineered MMLV RT with &gt;90% activity at 50°C/60 min. Working range 38–55°C. Synthesizes 100 bp–&gt;12 kb cDNA from 1 pg–5 µg total RNA. Reduced RNase H activity for full-length cDNA. Supplied at 200 U/µl with 5× Buffer.