<h2 dir="ltr">What Are AAV siRNA Negative Controls?</h2>
 AAV-delivered RNA interference (RNAi) is widely used for stable, long-term gene knockdown in hard-to-transfect primary cells and in vivo models. A matched scrambled AAV siRNA control is essential for distinguishing specific knockdown effects from non-specific AAV delivery effects and RNAi pathway activation. The right siRNA control helps validate gene-silencing results and reduce experimental bias. Choose a control designed for your RNA interference workflow. 
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Serotype-matched scrambled siRNA control viruses for tissue-specific normalisation, available across multiple AAV serotypes.</li>
<li>Scrambled siRNA control vectors suitable for cloning and propagation using ampicillin resistance (Amp).</li>
<li>RNAi negative controls for evaluating non-specific effects associated with AAV-delivered gene-silencing studies.</li>
<li>Viral delivery controls for assessing transduction-related cellular responses independently of target-gene knockdown.</li>
<li>High-titer scrambled control viruses designed to support consistent delivery across experimental cohorts.</li>
</ul>
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<h2>How to Choose an AAV siRNA Control</h2>
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Empty Vector vs. Scrambled siRNA Control
An empty AAV vector controls only for AAV delivery effects. Expression of RNAi constructs can influence cellular pathways independently of the target gene, making a scrambled siRNA control a more appropriate comparator for gene-silencing studies.
Serotype Matching
The control AAV should match the serotype of the experimental construct to ensure comparable tissue tropism and transduction characteristics, particularly for in vivo knockdown studies.
Promoter Matching
The control construct should use the same promoter as the experimental RNAi vector to ensure comparable expression levels and cellular processing.
Application Context
AAV RNAi controls are particularly important for in vivo and primary cell knockdown studies where transfection-based controls would not capture AAV-specific delivery effects.
<h2 dir="ltr"> Specifications Context</h2>
 ABM's Scrambled AAV siRNA Control Vector (Cat. iAAV01500) provides a matched control for AAV-delivered RNAi experiments. While empty vectors account for AAV delivery effects, scrambled siRNA controls additionally account for cellular responses associated with expression of the RNAi construct itself. These controls are particularly valuable for in vivo and primary cell knockdown studies, where AAV-mediated delivery and long-term expression are integral to the experimental design. Confirming knockdown efficiency by qPCR or Western blot in a pilot in vitro experiment before proceeding to in vivo studies is recommended, as RNAi performance can vary by target sequence, expression level, and cell type. Product availability reflects MBP's catalogue as of mid-2026. 
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to discuss the most appropriate AAV siRNA control constructs for your study and request a quote tailored to your research requirements.

siRNA Control

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Scrambled AAV siRNA Control Virus (Serotype 2)

Unit Quantity

Vector Map

Titer

Storage Condition

Shipping Condition

Scrambled AAV siRNA Control Virus (Serotype 3)

Scrambled AAV siRNA Control Virus (Serotype 4)

Scrambled AAV siRNA Control Virus (Serotype 5)

Scrambled AAV siRNA Control Virus (Serotype 6)

Scrambled AAV siRNA Control Virus (Serotype 7)

Certificate of Analysis

Scrambled AAV siRNA Control Virus (Serotype 8)

Scrambled AAV siRNA Control Virus (Serotype 9)

<h2 dir="ltr"> What are siRNA Lentiviral Negative Controls?</h2>
 Every well-designed RNAi experiment requires at least one matched negative control. Without proper control, it is impossible to determine whether an observed phenotype reflects specific target gene silencing, non-specific dsRNA effects, viral transduction artefacts, or off-target silencing by the guide strand. Meaningful gene-silencing results require proper experimental controls. Choose the siRNA control appropriate for your study.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Scrambled siRNA Control Vectors: Non-targeting siRNA lentivectors for negative control experiments in gene-silencing studies.</li>
<li>Scrambled siRNA Control Viruses:&nbsp; Ready-to-use lentiviral controls for validating RNAi workflows and transduction conditions.</li>
<li>GFP-Expressing Controls: Scrambled siRNA constructs with GFP reporters for monitoring transduction and expression.</li>
<li>Neomycin-Selectable Controls: Control vectors and viruses featuring neomycin resistance for stable cell selection.</li>
<li>Vector and Viral Formats: Available as both lentivectors and packaged lentiviruses to support diverse experimental workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose siRNA Negative Controls</h2>
 Control Design Type Non-targeting siRNA sequences are bioinformatically designed with no significant homology to any known human, mouse, or rat gene &mdash; entering RISC and activating RNAi machinery the same way as experimental sequences, providing a physiologically relevant baseline. Scrambled vs. Non-Targeting Scrambled controls rearrange the nucleotide sequence of an experimental siRNA while preserving base composition, but the rearranged sequence may inadvertently contain a seed region with off-target homology. Non-targeting designs with seed-region optimisation are the preferred approach. Vector Backbone Matching Non-targeting lentiviral controls should use the same vector backbone and promoter as the experimental, so differences between groups are attributable to sequence-specific effects rather than backbone, promoter, or selection marker differences. Community Standard Reference The pLKO.1 scramble control (Addgene 1864), deposited from the Sabatini lab, is the community standard negative control for TRC library-based experiments, cited in thousands of publications. Selection and Reporter Options Lentiviral negative controls are available with puromycin, hygromycin, blasticidin, and neomycin selection markers, as well as fluorescent reporter versions (GFP, RFP) for FACS-based selection. 
<h2 dir="ltr"> Specifications Context</h2>
 Microarray profiling studies have demonstrated that scrambled controls can exhibit off-target expression signatures differing from non-targeting controls &mdash; non-targeting designs validated with a minimum of three mismatches or gaps to all potential PAM-adjacent targets are the preferred standard for rigorous RNAi design. Best practice recommends including a non-targeting negative control, an empty vector control, and, where possible, a positive control targeting a constitutively expressed housekeeping gene (GAPDH, ACTB) to confirm knockdown conditions are functioning. For lentiviral screens, multiple distinct non-targeting controls should be distributed throughout the library to provide a statistically robust baseline. Validation by microarray or RNA-seq should show minimal differential gene expression (&lt;2-fold) versus untreated cells. 
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to get professional siRNA controls for your lab.