Name: Scrambled AAV siRNA Control Virus (Serotype 4)
SKU: iAAV01504
Price: 227.42 USD
Availability: InStock

<h2 dir="ltr">What Are AAV siRNA Negative Controls?</h2>
 AAV-delivered RNA interference (RNAi) is widely used for stable, long-term gene knockdown in hard-to-transfect primary cells and in vivo models. A matched scrambled AAV siRNA control is essential for distinguishing specific knockdown effects from non-specific AAV delivery effects and RNAi pathway activation. The right siRNA control helps validate gene-silencing results and reduce experimental bias. Choose a control designed for your RNA interference workflow. 
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Serotype-matched scrambled siRNA control viruses for tissue-specific normalisation, available across multiple AAV serotypes.</li>
<li>Scrambled siRNA control vectors suitable for cloning and propagation using ampicillin resistance (Amp).</li>
<li>RNAi negative controls for evaluating non-specific effects associated with AAV-delivered gene-silencing studies.</li>
<li>Viral delivery controls for assessing transduction-related cellular responses independently of target-gene knockdown.</li>
<li>High-titer scrambled control viruses designed to support consistent delivery across experimental cohorts.</li>
</ul>
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<h2>How to Choose an AAV siRNA Control</h2>
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Empty Vector vs. Scrambled siRNA Control
An empty AAV vector controls only for AAV delivery effects. Expression of RNAi constructs can influence cellular pathways independently of the target gene, making a scrambled siRNA control a more appropriate comparator for gene-silencing studies.
Serotype Matching
The control AAV should match the serotype of the experimental construct to ensure comparable tissue tropism and transduction characteristics, particularly for in vivo knockdown studies.
Promoter Matching
The control construct should use the same promoter as the experimental RNAi vector to ensure comparable expression levels and cellular processing.
Application Context
AAV RNAi controls are particularly important for in vivo and primary cell knockdown studies where transfection-based controls would not capture AAV-specific delivery effects.
<h2 dir="ltr"> Specifications Context</h2>
 ABM's Scrambled AAV siRNA Control Vector (Cat. iAAV01500) provides a matched control for AAV-delivered RNAi experiments. While empty vectors account for AAV delivery effects, scrambled siRNA controls additionally account for cellular responses associated with expression of the RNAi construct itself. These controls are particularly valuable for in vivo and primary cell knockdown studies, where AAV-mediated delivery and long-term expression are integral to the experimental design. Confirming knockdown efficiency by qPCR or Western blot in a pilot in vitro experiment before proceeding to in vivo studies is recommended, as RNAi performance can vary by target sequence, expression level, and cell type. Product availability reflects MBP's catalogue as of mid-2026. 
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to discuss the most appropriate AAV siRNA control constructs for your study and request a quote tailored to your research requirements.

siRNA Control

Selection-Drug Killing Curve

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Scrambled AAV siRNA Control Virus (Serotype 4)

Scrambled AAV siRNA Control Virus (Serotype 4) (iAAV01504) by Applied Biological Materials is a packaged AAV4 scrambled siRNA control at 10¹¹ GC/mL, supplied as 5 × 100 µl, for use as a serotype-matched non-targeting siRNA negative control in AAV4-delivered RNAi experiments. Shipped on dry ice; store at –80°C.