Immortalized Cells

Thawing Protocol

Cell Line Catalog (Volume 2)

Subculturing Protocol

Freezing Protocol

T2512 Integration-free wt1 iPSC

T2512 Integration-Free wt1 iPSC are human induced pluripotent stem cells generated by integration-free reprogramming, providing a genetically clean platform for disease modeling, differentiation, and drug screening. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

Human Dermal Fibroblasts-fetal Feeder Cells

T2002-1 Human Dermal Fibroblast &ndash; Fetal Feeder Cells are fetal human dermal fibroblasts optimized as a human-origin feeder layer for stem and progenitor cell culture, supporting attachment and growth in xeno-free/reduced-xeno workflows. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T2011 CB-iPSC-2

T2011 CB-iPSC-2 is a second clonal line of human cord blood-derived iPSC, providing an independent clone for experimental replication and clonal variation control alongside T2010 CB-iPSC. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2010 CB-iPSC

T2010 CB-iPSC are human induced pluripotent stem cells derived from cord blood, offering high reprogramming efficiency and reduced somatic mutation load for hematopoietic differentiation, disease modeling, and regenerative medicine research. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

Subculture Protocol

T2001 Mouse Embryonic Fibroblast Feeder Cells

T2001 Mouse Embryonic Fibroblast Feeder Cells are primary fetal MEF cells — the gold standard feeder layer for ESC and iPSC culture. Inactivate with mitomycin C or irradiation before use. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T2513 Human Sox17 Expressing Embryonic Stem Cells

T2513 Human SOX17 Expressing Embryonic Stem Cells are human ESC engineered to express SOX17, supporting definitive endoderm specification, directed differentiation into hepatic/pancreatic/pulmonary lineages, and organoid development research. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2503 LIF-Dependent Porcine iPSCs

T2503 LIF-Dependent Porcine iPSCs are Sus scrofa induced pluripotent stem cells maintained by LIF/STAT3 signaling, providing a large-animal iPSC model for translational biomedical and regenerative medicine research. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2502 Chicken iPSC

T2502 Chicken iPSC are induced pluripotent stem cells from Gallus gallus, providing a non-mammalian avian iPSC platform for comparative pluripotency, developmental biology, and avian virus-host interaction research. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2015-1 Human Dermal Fibroblasts &ndash; Fetal Feeder Cells are fetal human dermal fibroblasts providing a human-origin feeder layer for stem and progenitor cell culture in xeno-free or reduced-xeno workflows. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

<h2 dir="ltr"> What are NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; Systems are compact, single-use, multi-layer cell culture vessels manufactured from USP Class VI polystyrene for large-scale adherent cell culture. The BioFactory provides the growth kinetics and cell attachment characteristics of standard T-flasks, scaled up to growth areas from approximately 636 cm&sup2; (1-layer) to 25,440 cm&sup2; (40-layer) in a single vessel &mdash; making it the scale-up path from T-flask and roller bottle culture for vaccine production, monoclonal antibody manufacturing, viral vector production (lentivirus, AAV, adenovirus), and biopharmaceutical protein expression from adherent cell lines.&nbsp;
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>BioFactory systems: Multi-layer sterile cell culture vessels available with different chamber configurations, plug seal cap options, transfer caps, vent filters, and frost-resistant models for large-scale adherent cell culture.</li>
<li><a href="https://mbpinc.net/consumables/cell-culture/biofactory-systems/biofactory-system-accessories">BioFactory System Accessories:</a> Adaptor caps, overcaps, holders, vent filters, accessory packages, tubing sets, and preconfigured kits designed to support media handling and system operation.</li>
<li>Sterile fluid transfer components: CPC connectors, T- and Y-shaped connectors, sealing plugs, adaptor connectors, hose clamps, funnels, and filter/tubing assemblies for closed liquid transfer workflows.</li>
<li>Bioprocess tubing: Sterile C-Flex&reg; welding hose, SPT-50 hose, and inlet/outlet tubing compatible with BioFactory systems and media transfer applications.</li>
<li>Preassembled BioFactory configurations: Ready-to-use systems equipped with integrated vent filters, transfer tubing, or specialized cap assemblies to streamline cell culture setup.</li>
</ul>
<h2 dir="ltr"> How to Choose the Right BioFactory&trade; Layer Count</h2>
 Matching BioFactory Scale to Production Stage The 1-layer BioFactory (&asymp;636 cm&sup2;) is equivalent in surface area to approximately 8&ndash;9 standard T75 flasks &mdash; used for proof-of-concept scale-up studies transitioning from T-flask monolayer culture. The 2-layer BioFactory (&asymp;1,272 cm&sup2;) is the standard for pilot-scale viral antigen production and early-phase process development. The 5-layer (&asymp;3,180 cm&sup2;) and 10-layer (&asymp;6,360 cm&sup2;) formats suit mid-scale production runs for research-grade vaccine lots, transfection-derived protein, and pre-clinical viral vector batches. The 40-layer (&asymp;25,440 cm&sup2;) format is an industrial-scale format for commercial vaccine, mAb, and biopharmaceutical production. Closed Liquid Transfer and Contamination Reduction NEST BioFactory&trade; systems feature a narrow-mouth cap port compatible with aseptic tubing for closed liquid transfer &mdash; media, wash buffers, trypsin, and harvest supernatant can be introduced and removed through the tube port without opening the vessel. This closed-system operation eliminates the open-pour steps that create contamination risk in multi-layer roller bottle workflows and enables BSL-2 containment of pathogen-bearing cultures without biosafety cabinet&ndash;dependent open-handling. Wide-mouth caps allow direct media pouring for non-closed workflows. TC Treatment and Cell Compatibility All NEST BioFactory&trade; layers are TC-treated by vacuum plasma using the same surface treatment process as NEST cell culture T-flasks. NEST has published VERO cell culture data showing that cell morphology, adherence, and growth kinetics in the BioFactory are equivalent to those in standard T-flasks &mdash; cell attachment density, proliferation rate, and morphology are not altered by the scale-up. This linear scalability is the primary engineering advantage of multi-layer vessels over microcarrier bioreactors and packed-bed systems for adherent cell&ndash;derived products requiring 2D culture validation. Ultrasonic Welding and Particle Generation The BioFactory layers are joined by ultrasonic welding without chemical adhesives or extrinsic ingredients. NEST's welding line design minimizes particle generation at the weld joints, an important quality attribute for pharmaceutical production contexts where subvisible particle counts in harvest media must meet compendial limits. Automation and GMP Compatibility NEST BioFactory&trade; systems are compatible with automated media handling equipment. Multiple BioFactory units can be connected in series through the tubing ports for further scale-out. The double-bagged sterile packaging format supports GMP cleanroom entry requirements. NEST provides validation documentation packages (sterilization data, extractable/leachable certificates, biocompatibility reports) supporting GMP authorization for industrial cell culture production. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; Systems are manufactured from USP Class VI polystyrene in a Class 10,000 (ISO 7) dedicated cleanroom. All culture surfaces are TC-treated by vacuum plasma. Temperature range: approximately &minus;86 &deg;C to +64 &deg;C. E-beam sterilized to SAL 10⁻⁶; non-pyrogenic; double-bagged sterile packaging. Vent caps contain 0.22 &micro;m hydrophobic filters for gas exchange without contamination. VERO cell culture validation data available from NEST Scientific: seeding at 5 &times; 10⁴/mL in 2-layer BioFactory (400 mL) shows robust, even adherence and growth kinetics matching T-flask controls after 3 days. Cell lines validated: Vero, MRC-5, 2BS, 293T, L-929, and equivalent adherent production lines. NEST Scientific BioFactory&trade; systems across all layer configurations are carried by MBP. 
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Upgrade your cell culture workflow &mdash; <a href="https://mbpinc.net/contact-us">connect with the MBP team </a>to request pricing or product details.

Biofactory Systems

Multi-Layer Cell Culture Bioreactor

Unit Quantity

Recommended Medium Volume

Cell Growth Area

Shipping condition

Storage Boxes & Racks

<h2 data-section-id="1hv97bw" data-start="0" data-end="47">What Are Laboratory Storage Boxes and Racks?</h2>
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Laboratory storage boxes and racks are essential organizational systems used to store, organize, and protect cryogenic vials, microcentrifuge tubes, centrifuge tubes, and microscope slides across freezers, cold rooms, and benchtop environments. Storage boxes are typically lidded, grid-based containers used for long-term sample organization in ultra-low temperature conditions, while racks are open-frame supports used for active handling during laboratory workflows such as pipetting, vortexing, and reaction setup.
Cryogenic storage boxes are commonly designed to hold 81 vials (9&times;9 format) or 100 vials (10&times;10 format), and are compatible with storage conditions ranging from &ndash;196 &deg;C in liquid nitrogen vapor phase to +121 &deg;C for autoclave sterilization (depending on material). Tube racks, on the other hand, provide stable upright positioning of tubes during active benchwork and are not primarily intended for long-term freezer storage.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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Cryogenic storage boxes for vials and microtubes
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<li dir="ltr" aria-level="1">
Freezer storage boxes for -20&deg;C and -80&deg;C applications
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PCR tube racks for 0.2 mL tubes and strips
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Microcentrifuge tube racks for 0.5 mL to 2 mL tubes
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<li dir="ltr" aria-level="1">
Polypropylene (PP) autoclavable storage boxes
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<li dir="ltr" aria-level="1">
Stackable storage boxes for space-saving organization
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Hinged lid storage boxes for secure sample protection
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Color-coded racks for easy sample identification
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</ul>
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<h2 data-section-id="flyrii" data-start="1159" data-end="1199">How to Choose Storage Boxes and Racks</h2>
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Vial Format and Capacity
The first selection criterion is compatibility with tube size and grid layout. The most common format is the 81-place (9&times;9) box designed for 1.0&ndash;2.0 mL cryovials. Smaller or specialized formats may accommodate 5 mL tubes or different vial diameters depending on grid spacing. Proper matching ensures secure fit, easy retrieval, and consistent sample organization in high-density storage systems.
Temperature Range
Material selection determines allowable storage conditions. Polycarbonate cryogenic boxes are the most versatile, supporting temperatures from liquid nitrogen levels (&ndash;196 &deg;C) through &ndash;80 &deg;C freezer storage and up to autoclave sterilization at +121 &deg;C. Cardboard freezer boxes with moisture-resistant coating are designed for economical freezer storage and can withstand ultra-low temperatures, but they are not suitable for autoclaving or long-term moisture exposure.
Tube Rack Materials and Use
Polypropylene racks are widely used because they are autoclavable and chemically resistant, making them suitable for sterile workflows. ABS and polystyrene racks provide lower-cost alternatives for general laboratory bench use but are not designed for autoclave conditions. For freezer organization, stainless steel racks are often used to hold multiple cryoboxes within &ndash;80 &deg;C upright freezer systems, improving accessibility and spatial organization.
Slide Storage Boxes
Microscope slide storage boxes are designed to protect standard 25 &times; 75 mm slides. They are available in 25-, 50-, and 100-slide capacities. Polypropylene versions are autoclavable and suitable for sterilized workflows, while fiberboard or ABS versions are typically used for room-temperature archival storage and transport.
Color Coding and Sample Identification
Color-coded storage boxes are widely used in biobanking and high-throughput laboratories to quickly differentiate sample groups without opening boxes or reading labels at freezer temperatures. Common systems include multiple lid colors paired with alphanumeric grids inside the box, enabling precise sample tracking and retrieval in large inventories.
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<h2 data-section-id="1jl751w" data-start="4067" data-end="4092">Specifications Context</h2>
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Modern polycarbonate cryogenic storage boxes are engineered for extreme temperature stability, typically rated from &ndash;196 &deg;C to +121 &deg;C, allowing use across liquid nitrogen vapor phase storage, &ndash;80 &deg;C freezers, and autoclave sterilization cycles. Cardboard freezer boxes are coated to resist moisture ingress and are optimized for cost-efficient high-density storage in ultra-low temperature environments.
The 81-place (9&times;9 grid) polycarbonate cryobox remains the standard format in most biorepositories and clinical research facilities due to its compatibility with SBS footprint freezer systems and automated inventory tracking workflows. These systems are widely used in genomics, cell banking, and pharmaceutical research environments.
For product details, specifications, or bulk ordering support, <a href="https://mbpinc.net/contact-us">the MBP team can help you</a> choose the right storage solution for your laboratory needs.