<h2 dir="ltr"> What Are Cas9 Enzymes?</h2>
 Cas9 is the catalytic core of the CRISPR/Cas9 editing system. Sourced from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9), recombinant Cas9 proteins are expressed and purified to high purity for direct use in ribonucleoprotein (RNP) complexes &mdash; currently the most widely recommended format for mammalian cell editing due to rapid activity onset and minimised off-target effects compared to plasmid-based delivery. Genome-editing performance depends on the appropriate nuclease format. Choose the Cas9 enzyme that aligns with your editing strategy.
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<h2 dir="ltr">What you will find:</h2>
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NLS-Tagged Cas9 and saCas9 Nucleases: Recombinant proteins of high purity designed for quick nuclear localization and strong initiation of double-strand breaks (DSBs) at specific genomic sites.
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Precision Cas9 Nickase (H840A): Tailored variants created for dual-nicking approaches to improve genomic stability and notably minimize off-target alterations.
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Lyophilized Nuclease Preparations: Nuclease-free, shelf-stable proteins that offer optimal adaptability for reconstitution and high-throughput automated CRISPR-related processes.
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Catalytically Inactive Null Mutants: "Dead" Cas9 (dCas9) proteins designed for DNA-binding research and specific epigenetic modification without changing the original sequence.
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Cas12a (asCpf1) Nucleases: Alternative CRISPR systems that focus on T-rich PAM sequences to broaden the range of spatial transcriptomics and targetable genomic areas.
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GFP-Tagged Cas9 Proteins: Fluorescently marked nucleases developed for real-time assessment of transfection efficacy and visual verification of cellular absorption.
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Endotoxin-Free Enzyme Formulations: Highly pure mixtures crucial for preserving cell viability in sensitive lentiviral applications and primary cell modifications.
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<h2 dir="ltr"> How to Choose a Cas9 Enzyme</h2>
 PAM Requirement Wild-type SpCas9 recognises any genomic locus flanked by an NGG protospacer adjacent motif (PAM), occurring roughly every 8&ndash;12 bp in mammalian genomes. SaCas9 requires NNGRRT, though engineered PAM variants (KKH, KRH, KRKH) expand targetable space. Off-Target Sensitivity High-fidelity Cas9 variants carry substitutions in the recognition lobe that reduce non-specific DNA contacts, lowering off-target cleavage while maintaining on-target activity comparable to wild-type SpCas9 &mdash; recommended for therapeutic cell engineering and base editing. Editing Mechanism Wild-type SpCas9 generates a blunt double-strand break repaired by NHEJ (indels for knockout) or HDR (precise insertions). Nickase (D10A) cuts only one strand for paired-nick HDR strategies with reduced off-target indels. dCas9 carries mutations in both nuclease domains, enabling CRISPRa/CRISPRi gene regulation without DNA cleavage. AAV Compatibility SaCas9, at approximately 1 kb smaller than SpCas9, fits a complete Cas9 + sgRNA expression cassette within the ~4.7 kb AAV packaging capacity &mdash; enabling single-vector in vivo delivery without split-vector complexity. Storage and Handling Recombinant Cas9 proteins are supplied for stability during shipping and short-term storage at &minus;20 &deg;C or &minus;80 &deg;C. Repeated freeze-thaw cycles reduce activity; aliquoting upon receipt is recommended.
<h2 dir="ltr"> Specifications Context</h2>
 An RNP is a pre-formed complex of recombinant Cas9 protein and sgRNA; once electroporated, it acts immediately and is degraded within hours, reducing off-target cleavage and avoiding immune stimulation associated with plasmid or mRNA delivery.&nbsp;Recombinant Cas9 in RNP format is validated across HEK293, HeLa, iPSCs, T cells, primary neurons, and hepatocytes, though electroporation parameters typically require cell-type-specific optimisation. Product offerings reflect MBP's catalogue as of mid-2026. 
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Reach out to the supportive <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">team at MBP Inc.</a> now to obtain a quotation for the perfect Cas9 enzyme for your study.

Cas9 Enzymes

DataSheet

MSDS

saCas9 Nuclease NLS Protein

Unit Quantity

Cas Type

Cas Origin

Cas Protein Marker

Concentration

Format General

Storage Buffer

Storage Condition

Caution

Shipping Condition

saCas9 Nuclease NLS Protein (Lyophilized)

Endotoxin Level

saCas9 Nuclease Protein

saCas9 Null Mutant NLS Protein

saCas9 Null Mutant Protein

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
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<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

β-Gal Control

Vector Map

Titer

Note

Immortalized Cells

T2004 Immortalized Human Dermal Fibroblast-fetal Feeder Cells

T2004 Immortalized Human Dermal Fibroblast &ndash; Fetal Feeder Cells are an immortalized fetal human dermal fibroblast line providing a renewable, senescence-free human-origin feeder layer for xeno-free ESC/iPSC workflows; user-inactivate before feeder use. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2003 Immortalized Mouse Embryonic Fibroblast Feeder Cells

T2003 Immortalized MEF Feeder Cells are an immortalized mouse embryonic fibroblast line providing a renewable, senescence-free source of feeder cells for ESC/iPSC culture; user-inactivate before feeder use. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

Tumor cells

Thawing Protocol

Cell Line Catalog (Volume 2)

T1025 HepG2 Cells

HepG2 Cells (T1025) by MBP are non-tumorigenic human hepatoma cells with epithelial-like morphology and high proliferation rate. Used for metabolism and hepatotoxicity research and as a transfection host. BSL II. 1&times;106 cells / 1.0 mL, frozen. MTA required.

T1250 SUM149 Cells

SUM149 Cells (T1250) by MBP are a human triple-negative inflammatory breast cancer cell line used for studying tumor invasion, metastasis, cancer stem cell biology, and drug resistance. Human (H. sapiens), mammary, adherent epithelial-like. BSL II. 1&times;106 cells / 1.0 mL, frozen. MTA required.

Subculturing Protocol

Freezing Protocol

T2012 CB-iPSC-3

T2012 CB-iPSC-3 is the third clonal cord blood-derived human iPSC line, enabling multi-clone experimental designs to control for clonal variation in disease modeling, differentiation, and drug screening. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

United States

Texas

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