ABM Reagents

<h2 dir="ltr">What Are LAMP Polymerases?</h2>
 The defining enzyme of loop-mediated isothermal amplification is Bst DNA polymerase, isolated from Geobacillus stearothermophilus (formerly Bacillus stearothermophilus). Its high strand-displacement activity allows it to synthesise new DNA strands while displacing the existing complementary strand, without requiring thermal denaturation, the property that makes isothermal amplification possible. Choose standard Bst DNA polymerase for DNA-template LAMP; choose engineered Bst variants (Bst 2.0, Bst 3.0, or lyo-ready formats) for RT-LAMP applications requiring combined reverse transcriptase activity, faster reaction speed, or room-temperature-stable formats. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="6ukg4n" data-start="2051" data-end="2199">Strong strand-displacement DNA synthesis using Bst DNA polymerase variants, enabling rapid isothermal amplification without thermal cycling.</li>
<li data-section-id="7ycop5" data-start="2200" data-end="2330">High-efficiency Phi29 DNA polymerase systems supporting whole-genome amplification through processive strand displacement.</li>
<li data-section-id="18wphsf" data-start="2331" data-end="2463">Enzyme formats optimised for fast, constant-temperature nucleic acid amplification across LAMP and related isothermal methods.</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a LAMP Polymerase</h2>
 Strand-Displacement Activity Bst DNA polymerase's large fragment (Bst LF) provides the core strand-displacement activity that enables LAMP, operating at a temperature optimum near 65 &deg;C -- this activity allows continuous synthesis and primer annealing on single-stranded regions generated during amplification, without thermal cycling. Reverse Transcription Capability Engineered variants such as Bst 3.0 may offer enhanced reverse transcription capability, enabling RT-LAMP workflows by supporting RNA-to-cDNA conversion within the same isothermal reaction environment. Reaction Speed Optimised Bst formulations can support rapid amplification in LAMP reactions, with performance depending on primer design, template quality, and reaction conditions. Format: Liquid vs. Lyophilised Lyo-ready (lyophilised) formats support room-temperature shipping and storage, relevant for field-deployable or point-of-need applications where cold-chain logistics are impractical. Standard liquid formulations require frozen storage but may offer cost advantages for laboratory-based use. Pairing with Reverse Transcriptase For RT-LAMP using polymerases without intrinsic RT activity, pairing with a reverse transcriptase whose temperature optimum overlaps with the Bst polymerase's working temperature (commonly 60-65 &deg;C) helps maintain the isothermal format -- engineered high-thermostability reverse transcriptases are better suited to this than standard AMV or M-MLV enzymes with lower temperature optima. 
<h2 dir="ltr"> Specifications Context</h2>
 Bst DNA polymerase large fragment drives LAMP through strand-displacement activity at its temperature optimum near 65 &deg;C; because LAMP uses this single-temperature synthesis instead of thermal denaturation, the enzyme's strand-displacement capability -- rather than thermostability across a wide cycling range as with Taq -- is the key performance attribute. When combining AMV or M-MLV reverse transcriptases (with lower native temperature optima) with Bst polymerase for RT-LAMP, the temperature mismatch can require a two-temperature protocol, undermining the isothermal format; this has driven engineering efforts producing polymerases such as Bst 3.0 with intrinsic reverse transcriptase activity functioning at the Bst temperature optimum. Comparative testing has shown lyo-ready Bst DNA polymerase formulations can provide significantly higher RT-LAMP reaction speed than some other commercially available Bst polymerases when tested with the same RNA targets and primer sets. LAMP polymerases are typically stored at &minus;20 &deg;C (liquid formats) or at room temperature (lyo-ready formats per product specification); lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to find polymerases suited to your LAMP and isothermal amplification workflows.

Polymerases

MSDS - Ultra-Pure Phi29 DNA Polymerase

Phi29 DNA Polymerase

E014 by Applied Biological Materials is Ultra-Pure Phi29 DNA Polymerase, 100 µL (~200 reactions) with 5× Reaction Buffer (0.5 µg/µL BSA). Powerful strand-displacement + 3′→5′ proofreading. Isothermal RCA, MDA, WGA. Ultra-pure: TaqMan qPCR DNA contamination verified. Store at −20°C.

Unit Quantity

Format General

Storage Buffer

Storage Condition

Caution

Shipping Condition

<h2 dir="ltr">What Are Organelle Labeling Lentiviral Particles?</h2>
 Organelle labeling lentiviral particles are ready-to-transduce viral stocks that deliver a fluorescent protein (GFP or RFP) fused to a validated subcellular targeting sequence. After stable integration, transduced cells express a fluorescent marker localised to a specific organelle &mdash; providing a heritable, non-toxic alternative to chemical dyes for subcellular imaging. Cellular imaging studies depend on accurate organelle targeting. Choose a labeling system that supports clear visualization and analysis.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mitochondria labeling viruses for monitoring cellular metabolism and energy dynamics in live-cell studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Nucleus labeling viruses for gene expression analysis and nuclear localization tracking in mammalian cells</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endoplasmic reticulum labeling viruses for studying folding, synthesis, and intracellular transport pathways of proteins</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Golgi apparatus labeling viruses for protein processing and for vesicle trafficking visualization</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Plasma membrane labeling of viruses for cell interaction studies, receptor localization, and membrane dynamics</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Lysosome labeling viruses for autophagy and degradation pathway-related applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Peroxisome labeling viruses for oxidative stress response and metabolic studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Cytoskeleton labeling viruses for cell morphology analysis and structural organization studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Autophagosome labeling of viruses for cellular recycling pathway and autophagy flux studies</li>
</ul>
<h2 dir="ltr"> How to Choose an Organelle Labeling Lentivirus</h2>
 Target Organelle Available targeting constructs cover mitochondria, endoplasmic reticulum, nucleus, Golgi apparatus, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; select based on the structure of interest in your experiment. Fluorophore Choice GFP- and RFP-based constructs allow flexibility for multiplexed imaging &mdash; pairing an organelle marker with a second fluorescent reporter (e.g. a protein-of-interest fusion) in the red or green channel respectively. Stable vs. Transient Labeling Needs Lentiviral delivery produces stable, integrated reporter expression maintained across cell divisions &mdash; essential for long-term time-lapse imaging, drug treatment time courses, or generation of reporter cell line banks. Cytotoxicity Considerations Unlike chemical dyes (MitoTracker, LysoTracker, ER-Tracker), which can be cytotoxic at working concentrations and require repeated application, lentiviral organelle markers avoid repeated dye loading and chronic toxicity in long-term experiments. Multiplexing Strategy When combining an organelle marker with other fluorescent reporters, select spectrally distinct fluorophores to avoid channel overlap in fluorescence microscopy or flow cytometry. 
<h2 dir="ltr"> Specifications Context</h2>
 Organelle labeling lentiviral particles sourced from Applied Biological Materials (ABM) are supplied as ready-to-transduce stocks, eliminating the need for in-house viral production. Stable genomic integration enables longitudinal analysis of organelle morphology and dynamics across cell passages, treatments, and differentiation time courses. All lentiviral workflows require institutional biosafety committee (IBC) approval, with BSL-2 containment standard for third-generation systems. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>of MBP to confirm availability for specific organelle targets and fluorophore combinations.

Organelle Labeling Viruses

Important Considerations for Lentiviruses

Plasma Membrane Labeling Lentivirus (Farnesyl Signal)(GFP)

LVP709 by Applied Biological Materials is a pre-packaged lentivirus (2 × 100 µl) expressing farnesylated GFP (CAAX box) for stable, specific green fluorescence labeling of the plasma membrane inner leaflet in transduced mammalian cells. For live-cell membrane morphology imaging, cell-cell contact studies, and two-color co-localization with RFP-labeled organelles. Store at &minus;80°C.

Vector

Titer	

Plasma Membrane Labeling Lentivirus (Farnesyl Signal)(RFP)

LVP710 Plasma Membrane Labeling Lentivirus (Farnesyl Signal)(RFP) by Applied Biological Materials delivers farnesyl CAAX-RFP for stable red fluorescent plasma membrane labeling via lipid anchor targeting. Compatible with TRITC/561 nm filter sets; spectrally compatible with GFP-based intracellular markers. Store at &minus;80&deg;C.

Plasma Membrane Labeling Lentivirus (LCK)(GFP)

LVP712 Plasma Membrane Labeling Lentivirus (LCK)(GFP) by Applied Biological Materials delivers a LCK-GFP fusion that anchors GFP to the inner plasma membrane leaflet, enabling stable, genetically encoded green fluorescent cell-surface labeling in transduced mammalian cells. Compatible with standard 488 nm imaging systems; store at &minus;80&deg;C.

Plasma Membrane Labeling Lentivirus (LCK)(RFP)

LVP713 by Applied Biological Materials is a pre-packaged lentivirus (2 × 100 µl) expressing RFP anchored to the plasma membrane inner leaflet via an LCK myristoylation + palmitoylation signal for stable red fluorescence plasma membrane labeling. RFP complement to LVP709 (Farnesyl-GFP). For live-cell membrane morphology imaging, immune synapse studies, and two-color co-localization with GFP-labeled organelles. Store at &minus;80°C.

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

AAV Infection Guideline

Lentivector Packaging Protocol

Lentiviral Vector Amplification Protocol

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivirus Infection Protocol

pLenti-CMV-GFP-2A-Puro-Blank Vector

LV590 by Applied Biological Materials is a 1.0 µg insert-empty lentiviral plasmid control vector with a CMV promoter, co-expressing GFP and Puromycin resistance via a 2A self-cleaving peptide. Matched negative control for the pLenti-CMV-CBH-GFP-2A-Puro expression backbone. Requires lentiviral packaging. Store at −20°C or below.

Vector Map

Note

<h2 dir="ltr"> What Are Lentiviral Cloning Vectors?</h2>
 The transfer plasmid is the heart of every lentiviral construct &mdash; it defines the transgene cargo, promoter driving expression, selection marker, and structural LTR elements that enable stable genomic integration. Selecting the right cloning vector backbone is the first and most consequential decision in any lentiviral project.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif;">Expression vectors featuring CMV, EF1&alpha;, PGK, and UbC promoter configurations for transgene expression studies</li>
<li>Fluorescent reporter vectors incorporating GFP, RFP, and CFP for cell tracking and visualization</li>
<li>Promoterless vectors for promoter characterization and regulatory element analysis</li>
<li>HA-tag and His-tag expression vectors for protein detection and purification workflows</li>
<li>Bicistronic and tricistronic vector systems for multi-gene expression applications</li>
<li>miRNA- and siRNA-compatible cloning vectors for RNA interference studies</li>
<li>Functional vectors including selectable-marker and HSVtk-based constructs for specialized research applications</li>
</ul>
<h2 dir="ltr"> How to Choose a Lentiviral Cloning Vector</h2>
&nbsp;
Vector Design
Expression vectors, reporter vectors, promoterless systems, tagged constructs, and multicistronic backbones are designed for different experimental objectives. Vector selection should be guided by the intended application, transgene design, and downstream analysis requirements.
Promoter Selection
Promoters influence the strength and pattern of transgene expression. Available vector configurations include CMV, EF1&alpha;, PGK, and UbC promoters, as well as promoterless backbones for studies focused on promoter activity and regulatory elements.
Reporter Requirements
Fluorescent reporters such as GFP, RFP, and CFP enable visualization of transduced cells, monitoring of expression, and assessment of transduction efficiency. Reporter selection should align with the imaging platform and experimental workflow.
Expression Configuration
Single-gene, bicistronic, and tricistronic vector designs provide different levels of flexibility for co-expression studies. Multi-cassette systems can simplify experiments requiring coordinated expression of multiple genes or reporter elements.
Insert Size Considerations
Lentiviral vectors can accommodate relatively large expression cassettes compared with several other viral delivery systems. However, larger inserts may reduce viral production efficiency and should be evaluated during vector design and validation.
<h2 dir="ltr"> Specifications Context</h2>
 Modern research lentiviral vectors are commonly designed with self-inactivating (SIN) long terminal repeats (LTRs), an important biosafety feature that reduces promoter activity within the integrated viral LTR following transduction. Many lentiviral vector systems also incorporate regulatory elements that support efficient transgene expression and vector performance. Vector architecture, promoter selection, reporter configuration, and insert size can all influence downstream cloning, viral production, and expression outcomes. Product availability reflects MBP's catalogue as of mid-2026. 
The search for reliable lentiviral cloning vectors is over. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and find the perfect match today.

Cloning Vectors

pLenti-CMV-RFP-2A-Puro Vector

LV084 by Applied Biological Materials is a 10 µg pLenti-CMV-RFP-2A-Puro lentiviral expression vector. CMV promoter co-expressing RFP and Puromycin resistance via T2A self-cleaving peptide. For stably transduced cell lines with concurrent RFP tracking and Puromycin selection. Pre-packaged blank: LVP691. Requires packaging. Store at −20°C or below.

Full Sequence of LV591

Full Sequence of LV590

Full Sequence of LV588

Full Sequence of LV589

pLenti-CMV-RFP-2A-Puro-Blank Vector

LV591 by Applied Biological Materials is a 1.0 µg insert-empty lentiviral control plasmid with CMV promoter co-expressing mKate2 far-red RFP (excitation ∼588 nm / emission ∼633 nm) and Puromycin resistance via a 2A peptide. Matched RFP-channel negative control for CMV-CBh-RFP-2A-Puro backbone experiments. Requires lentiviral packaging. Shipped on ice pack; store at −20°C or below.

pLenti-EF1a-Blank Vector

LV588 by Applied Biological Materials is a 1.0 µg insert-empty EF1α-SV40-Puro lentiviral blank control plasmid (Lenti-EF1a-Blank-SV40-Puro). Matched plasmid-level negative control for EF1α-Puro backbone experiments. Pre-packaged virus: LVP688. Requires lentiviral packaging. Store at −20°C or below.

pLenti-III-Blank Vector

LV587 by Applied Biological Materials is a 1.0 µg insert-empty lentiviral control plasmid with a CMV promoter (blank cassette) and SV40-driven Puromycin resistance. Matched negative control for CMV-SV40-Puro backbone lentiviral expression experiments. Requires lentiviral packaging. Store at −20°C or below.

<h2 dir="ltr">What Are miRNA Lentiviral Negative Controls?</h2>
 MicroRNAs (miRNAs) are approximately 22-nucleotide non-coding RNAs that regulate gene expression post-transcriptionally by binding to the 3&prime; UTR of target mRNAs, triggering mRNA degradation or translational repression via the RNA-induced silencing complex (RISC). Lentiviral delivery of miRNA expression constructs enables stable, long-term modulation of miRNA activity &mdash; essential where transient transfection approaches are insufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Control Viruses models used in miRNA overexpression studies, for negative control normalization
</li>
<li dir="ltr" aria-level="1">
Validation of 3'UTR target utilizing bioluminescent-based reporter assays.
</li>
<li dir="ltr" aria-level="1">
miRNA-Off Control Lentiviruses used to baseline microRNA knockdown effects.
</li>
<li dir="ltr" aria-level="1">
Blank 5&rsquo; UTR reporter vector optimized for gene expression normalization and translational regulation studies&nbsp;
</li>
<li dir="ltr" aria-level="1">
Reporter Stable Cell Lines models with Luciferase or GFP markers for reliable assay standardization.
</li>
</ul>
<h2 dir="ltr"> How to Choose a miRNA Negative Control</h2>
 Experiment Type Matching miRNA mimic (gain-of-function) experiments require a non-targeting miRNA mimic control. miRNA inhibitor/antagomiR (loss-of-function) experiments require a non-targeting inhibitor control. Lentiviral miRNA expression requires an empty vector or scrambled miRNA sequence. Mimic Control Design A non-targeting miRNA mimic control is a double-stranded RNA molecule resembling a mature miRNA duplex but with no significant homology to any known miRNA target in human, mouse, or rat transcriptomes &mdash; entering RISC the same way as a functional mimic. Inhibitor Control Design Non-targeting miRNA inhibitor (antagomiR) controls are single-stranded antisense sequences that enter the relevant cellular compartment without blocking any known miRNA, establishing the baseline for inhibitor-treated versus non-inhibitor comparisons. Vector Backbone for Lentiviral Delivery Stable lentiviral expression of miRNAs is used for long-term gain-of-function studies; the matched non-targeting lentiviral control must use the same promoter and vector backbone as the experimental construct to isolate sequence-specific effects. Inducible Format Availability Tet-On (doxycycline-responsive) non-targeting miRNA lentiviral controls are available for experiments requiring temporal control, used alongside inducible experimental constructs to control for doxycycline treatment itself. 
<h2 dir="ltr"> Specifications Context</h2>
 miRNA controls are designed to reflect the endogenous processing pathway of microRNAs, including Drosha and Dicer-mediated maturation before RISC loading. Because siRNA and miRNA constructs enter the RNA interference pathway differently, pathway-matched controls are important for accurate experimental interpretation. Non-targeting miRNA controls help distinguish sequence-specific effects from background effects associated with vector delivery and miRNA pathway activation. Available formats include lentiviral vectors, packaged viruses, reporter constructs, and stable cell line controls for miRNA and UTR-based studies. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and request a quote for efficient miRNA control requirements.

miRNA Control

QC report and Full sequence of the vector

pLenti-III-mir Control Vector

m003 by Applied Biological Materials is a pLenti-III-mir Control Vector — blank miRNA insert + GFP reporter lentiviral plasmid. No-miRNA baseline GFP control for Lenti-III-mir series miRNA overexpression experiments. Pre-packaged virus: m002. Requires lentiviral packaging. Store at −20°C or below.

pLenti-III-mir-GFP Cloning Vector

m016 by Applied Biological Materials is a third-generation lentiviral transfer vector for stable miRNA expression in mammalian cells, featuring an integrated GFP reporter for transduction and expression monitoring. Suitable for miRNA overexpression, RNAi-based gene knockdown, and miRNA functional screening. Compatible with third-generation VSV-G lentiviral packaging systems.