ABM Reagents

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
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<h2 dir="ltr">What you will find:</h2>
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<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
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Targeted fluorescent labeling of specific cellular structures using viral delivery.
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Stable expression of organelle-specific markers for long-term live-cell imaging.
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Compatible with fluorescence microscopy for dynamic cellular imaging applications.
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<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
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<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
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Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
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<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

pBABE-puro-CCDC68

AX0010003 pBABE-puro-CCDC68 by Applied Biological Materials is a retroviral expression plasmid (pBABE backbone) encoding CCDC68 (Coiled-Coil Domain Containing 68) with Puromycin resistance for stable overexpression in mammalian cells, for tumor suppressor function, cell cycle, and DNA damage response studies. Store at &minus;20&deg;C or below.

Unit Quantity

Storage Condition

Shipping Condition

pBABE-puro-ELF3-flag

AX0010006 by Applied Biological Materials is a pBABE-puro retroviral expression plasmid (1.0 µg) encoding human ELF3 (ESE-1 ETS transcription factor) with a C-terminal FLAG tag and Puromycin resistance. For ELF3 overexpression, anti-FLAG ChIP of ELF3 target genes, protein-protein interaction studies, and stable retroviral cell line generation with Puromycin selection.

Vector

pBABE-puro-FHL2

AX0010002 pBABE-puro-FHL2 by Applied Biological Materials is a retroviral expression plasmid (pBABE backbone) encoding FHL2 (Four-and-a-Half LIM domain protein 2) with Puromycin resistance for stable overexpression in transduced or transfected mammalian cell lines. Store at &minus;20&deg;C or below.

pBABE-puro-MFSD2A

AX0010001 by Applied Biological Materials is a pBABE-puro retroviral expression plasmid (1.0 µg) encoding human MFSD2A — a sodium-dependent LPC transporter essential for brain DHA/omega-3 uptake at the blood-brain barrier and BBB permeability regulation. Puromycin resistance for stable retroviral cell line generation. For MFSD2A overexpression, BBB transport assays, transcytosis studies, and rescue experiments in MFSD2A-deficient models.

pBABE-puro-TIMP3

AX0010005 pBABE-puro-TIMP3 by Applied Biological Materials is a retroviral expression plasmid (pBABE backbone) encoding TIMP3 (Tissue Inhibitor of Metalloproteinases 3) with Puromycin resistance for stable overexpression in transduced or transfected mammalian cells. Store at &minus;20&deg;C or below.

pBABE-puro-TPM1

AX0010004 by Applied Biological Materials is a pBABE-puro retroviral expression plasmid (1.0 µg) encoding human TPM1 (Tropomyosin 1, alpha-chain) with Puromycin resistance. For stable TPM1 overexpression via retroviral delivery in dividing mammalian cells. Applicable to actin cytoskeleton biology, cancer cell motility, mechanotransduction, and TPM1 rescue experiments in knockdown/knockout models.

<h2 dir="ltr">What Is a Mycoplasma PCR Detection Kit?</h2>
 A Mycoplasma PCR detection kit provides primers, master mix, ultrapure water, and a positive control for rapid PCR-based screening of cell culture samples for Mycoplasma contamination. Because Mycoplasma species lack a cell wall and do not produce visible turbidity or media discoloration, contamination frequently goes undetected without periodic molecular testing, making these kits a standard component of cell culture quality assurance programmes. Run a Mycoplasma PCR detection kit periodically on actively maintained cell lines and before banking, sharing, or publishing results from a cell line as routine quality assurance against a contaminant that produces no visible signs. 
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<h2 dir="ltr">What you will find:</h2>
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Detection &amp; Elimination Kits: All-encompassing systems such as Mycoplasma Pro crafted to accurately identify contamination while also enabling complete eradication of infected cultures.
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PCR Detection Kits: Standardized, validated reagents that provide a quick and economical solution for regular laboratory testing and quality assurance.
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Mycoplasma gDNA Kits: Tailored genomic DNA extraction solutions specifically designed for isolating low-abundance mycoplasma templates from intricate biological specimens.
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<h2 dir="ltr"> How to Choose a Mycoplasma PCR Detection Kit</h2>
 Species/Strain Coverage Kits vary in the breadth of Mycoplasma genera and species detected -- some formats detect over 200 strains, while enhanced ("Pro"-type) formats may extend coverage to 300+ strains, targeting conserved regions shared across common contaminant species such as M. arginini, M. hyorhinis, M. orale, and others. Sample Preparation Requirements Many Mycoplasma PCR detection kits allow direct addition of cell culture media to the PCR reaction without a separate DNA extraction step, simplifying the workflow and reducing time-to-result -- confirm whether your chosen kit requires extraction or supports direct sample addition. Internal Control Availability Enhanced kit formats may include an internal amplification control, providing additional confidence that a negative result reflects true absence of contamination rather than a failed or inhibited PCR reaction -- particularly valuable for samples with potential PCR inhibitors. Detection Sensitivity Standard kits can detect contamination at typical levels found in actively growing contaminated cultures; ultra-sensitive qPCR-based Mycoplasma detection formats (a related but distinct product type) can detect as few as 10 genome copies directly from media for earlier-stage contamination detection. Result Interpretation A positive result is typically indicated by a PCR product in a defined size range (commonly in the 260-550 bp range depending on the specific primer design), distinguishable from the positive control band; a clean no-template control validates assay specificity for each run. 
<h2 dir="ltr"> Specifications Context</h2>
 Mycoplasma PCR detection kits use primers highly specific to conserved rDNA regions across Mycoplasma genomes, enabling a single primer mix to detect dozens to hundreds of species and strains -- including commonly encountered laboratory contaminants - in one reaction. The streamlined workflow (combine sample with master mix and primer mix, run PCR, analyse by gel electrophoresis) typically delivers results within 1-2 hours, in contrast to culture-based Mycoplasma testing methods that can take days to weeks. A positive result appears as a distinct PCR product within the kit's expected size range, while the included positive control confirms the reaction performed correctly even when test samples are negative. Routine screening intervals vary by institutional policy, but testing before banking a new cell line, after receiving cells from an external source, and periodically during long-term culture are common checkpoints. Mycoplasma PCR detection kits are typically stored at &minus;20 &deg;C; positive controls are included with the kit. 
Safeguard the integrity of your culture&mdash;<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">reach out to the MBP team</a> now for a quote on our expert Mycoplasma detection services.

Mycoplasma PCR Detection Kit

MSDS

Flyer

G238-List of Detectable Mycoplasma Species/Strains

PCR Mycoplasma Detection Kit

PCR Mycoplasma Detection Kit (G238) by Applied Biological Materials is a 100-reaction kit detecting 200+ Mycoplasma strains in under 2 hours with no DNA isolation. Includes BlasTaq™ MasterMix with gel loading dye, Primer Mix, Positive Control, and Nuclease-Free H₂O. ISO 13485:2016 MDSAP certified. Outperforms Sigma and Lonza kits (Russell et al., 2020). Store at –20°C; stable 2 years.

<h2 dir="ltr"> What Is a Taq PCR Master Mix?</h2>
 The 2x Taq PCR master mix format has become the standard for high-throughput molecular biology workflows because it combines reliability, convenience, and reproducibility in a single-tube format. By pre-optimising the concentrations of all PCR components, master mixes reduce pipetting steps and experimental variability compared to assembling reactions from individual components. This simplifies workflow setup and improves reproducibility. Select a formulation designed for your amplification needs.
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<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr">High-Purity Enzymes: Versatile independent polymerases, such as the Azura&trade; and Accuris series, crafted for adaptable reaction adjustments and enhanced processivity with various DNA templates.</li>
<li dir="ltr">Full Reaction Solutions: All-inclusive systems such as the PCR Sure&trade; Kit that feature optimized dNTPs and buffers, offering a ready-to-use option for labs requiring dependable high-throughput performance and consistent outcomes.</li>
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<h2 dir="ltr"> How to Choose a Taq PCR Master Mix</h2>
 Standard vs. Dye-Containing Standard 2x master mixes require a separate loading dye addition before gel electrophoresis. Dye-containing master mixes include loading dye in the formulation, allowing direct gel loading after amplification &mdash; streamlining routine band-confirmation workflows. Component Pre-Optimisation A 2x Taq PCR master mix is a pre-formulated, double-concentrated solution containing Taq DNA polymerase, dNTPs (typically 200 &micro;M each at 1x), MgCl2, and PCR buffer at optimised concentrations &mdash; requiring only template and primer addition to set up a reaction. Throughput Scale High-throughput genotyping and colony screening benefit most from master mix consistency across multi-well plates, where individual component pipetting would introduce variability and time cost. Storage and Stability Master mixes are typically stable at &minus;20 &deg;C with multiple freeze-thaw cycles, though some dye-containing formulations may have slightly different stability profiles &mdash; check product-specific storage recommendations. Compatibility with Existing Protocols When switching from individual-component reactions to a master mix format, confirm that the pre-set MgCl2 and dNTP concentrations are compatible with your existing primer designs and templates, or adjust accordingly. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x Taq PCR master mix is set up by combining the master mix with template DNA and primers &mdash; typically at a 1:1 ratio of master mix to combined template/primer/water volume, achieving the final 1x working concentration. This format has become the standard for high-throughput molecular biology because it reduces pipetting steps and lot-to-lot variability across multi-well plate setups, important for reproducibility in genotyping and screening pipelines. Master mixes are typically stored at &minus;20 &deg;C with documented multiple freeze-thaw tolerance. When transitioning a protocol from individual-component PCR to a master mix format, running both formulations side-by-side on the same template and primer set is a useful validation step, confirming that the pre-set MgCl2 and dNTP concentrations in the master mix produce comparable yield and specificity to the previously optimised individual-component reaction before fully adopting the new format across a workflow. Product availability reflects MBP's catalogue as of mid-2026. 
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Enhance your amplification outcomes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team </a>now for a quote on our expert Taq Polymerase offerings.

Taq PCR Master Mixes

DataSheet

PCR Product Guide

PCR Sure™ Kit

G065 by Applied Biological Materials is the PCR Sure&trade; Kit (8 × 10 rxn) — eight distinct PCR formulations for systematic troubleshooting of difficult, failed, or non-specific PCR amplifications. Addresses GC-rich templates, long amplicons, secondary structure, and primer non-specificity. Add template + primers to each formulation; compare results to identify the optimal condition. Consult the PCR Product Guide to scale up with the best-matching abm PCR reagent.

<h2 data-section-id="1go10h8" data-start="173" data-end="215">What are classic cell culture media?</h2>
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Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture since the mid-20th century: DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's Nutrient Mixtures F-10 and F-12, IMDM (Iscove's Modified Dulbecco's Medium), and the combination formulation DMEM/F-12. Each is manufactured according to its originally published formula, with modifications limited to those necessary for consistent performance and stability of the medium over its shelf life. These media supply the amino acids, vitamins, glucose, salts, and (in most formulations) phenol red pH indicator that form the nutritional backbone of essentially all subsequent specialty, serum-free, and Advanced media derivatives.
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<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr" role="presentation">Selective Mammalian Antibiotics including Puromycin, Geneticin, and Hygromycin B for stable development of cell lines.</li>
<li dir="ltr" role="presentation">Broad-Spectrum Bactericidal Agents for routine contamination prevention, including Penicillin/Streptomycin and Gentamicin&nbsp;</li>
<li dir="ltr" role="presentation">Prokaryotic Selection Reagents for effective bacterial transformation, including Ampicillin, Kanamycin, Chloramphenicol, and Tetracycline&nbsp;</li>
<li dir="ltr" role="presentation">Antifungal and Antimycotic Solutions to maintain sterile cultures, including combined 100X cocktails and Amphotericin B (Fungizone)&nbsp;</li>
<li dir="ltr" role="presentation">Specialized Small Molecules for targeted biochemical assays, including Ganciclovir, Rapamycin, and Actinomycin D&nbsp;</li>
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<h2 data-section-id="1mfajxk" data-start="1218" data-end="1252">How to choose a classic medium</h2>
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DMEM vs MEM
DMEM contains up to four times the amino acid and vitamin concentration of original Eagle's MEM, along with higher glucose levels, making it suitable for cell types with elevated nutrient demands &mdash; DMEM is broadly applicable across mammalian, insect, and plant cell phenotypes including primary cells, stem cells, and transformed lines. MEM remains widely used for monolayer culture of many standard cell types, with optional Hanks' or Earle's salt formulations and a calcium-free modification that permits suspension growth.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640, developed at the Roswell Park Memorial Institute for culturing human leukemic cells, is optimized for non-adherent cell types &mdash; lymphocytes and other blood cells &mdash; and contains glutathione plus higher biotin, B12, and PABA concentrations than DMEM or MEM. Despite its hematopoietic origin, RPMI 1640 supports a broad range of adherent lines (HeLa, T47D, RENCA, CT26.WT) when properly supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were originally developed for CHO cell clones, HeLa cells, and mouse L-cells, and remain suited to hepatocyte culture, viral fusion experiments, and toxicity assays, with formulations available for use with or without serum. Coon's modification of Ham's F-12 roughly doubles the amino acid and pyruvate content and adds ascorbic acid, developed specifically for hybrid cells produced by viral fusion.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 (1:1 mixture) combines DMEM's glucose, amino acid, and vitamin richness with Ham's F-12's zinc, putrescine, hypoxanthine, and thymidine, producing an extremely rich complex medium used for MDCK cells, glial cells, fibroblasts, and human endothelial cells &mdash; and serving as the basal medium for many serum-free Advanced formulations used in organoid and stem cell work.
IMDM for high-density rapid proliferation
IMDM further enriches DMEM with additional amino acids and vitamins, substituting potassium nitrate for ferric nitrate and adding HEPES and sodium pyruvate. IMDM's sodium bicarbonate buffer system (5&ndash;10% CO₂) and enriched composition make it well suited for rapidly proliferating, high-density cultures including Jurkat, COS-7, and macrophage cells.
Liquid vs. powder format
Liquid classic media are ready to use (after appropriate FBS and supplement addition) and include sodium bicarbonate in the formulation. Powdered media are manufactured without sodium bicarbonate, since it tends to off-gas in powder form &mdash; 3.7 g/L sodium bicarbonate must be added upon reconstitution, followed by sterile filtration. Powder formats offer cost and shipping-weight advantages for high-volume labs preparing their own media.
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<h2 data-section-id="17i0pj" data-start="5035" data-end="5061">Specifications context</h2>
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Classic media are manufactured according to originally published formulas, with stability-related modifications documented per product. DMEM's sodium bicarbonate buffer system at 3.7 g/L is standard across liquid formulations; powdered formats require this addition upon reconstitution along with 0.22 &micro;m sterile filtration. As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the three most widely specified classic media across academic and biomanufacturing labs, with IMDM increasingly used for high-density suspension applications in bioprocessing.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents (GlutaMAX, NEAA, sodium pyruvate, HEPES), see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get highly efficient microbial control solutions for your lab.

Antibiotics

Penicillin/Streptomycin Solution

Penicillin/Streptomycin Solution (G255) by Applied Biological Materials — 100X concentrate, 10,000 units/mL penicillin and 10,000 µg/mL streptomycin in WFI, 0.22 µm filtered, sterility tested. 100 mL. Add 1 mL per 99 mL basal medium. Stable 2 years at –20°C.

Usage

Sterility

<h2 dir="ltr">What Are Organelle Labeling Lentiviral Particles?</h2>
 Organelle labeling lentiviral particles are ready-to-transduce viral stocks that deliver a fluorescent protein (GFP or RFP) fused to a validated subcellular targeting sequence. After stable integration, transduced cells express a fluorescent marker localised to a specific organelle &mdash; providing a heritable, non-toxic alternative to chemical dyes for subcellular imaging. Cellular imaging studies depend on accurate organelle targeting. Choose a labeling system that supports clear visualization and analysis.
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<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mitochondria labeling viruses for monitoring cellular metabolism and energy dynamics in live-cell studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Nucleus labeling viruses for gene expression analysis and nuclear localization tracking in mammalian cells</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endoplasmic reticulum labeling viruses for studying folding, synthesis, and intracellular transport pathways of proteins</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Golgi apparatus labeling viruses for protein processing and for vesicle trafficking visualization</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Plasma membrane labeling of viruses for cell interaction studies, receptor localization, and membrane dynamics</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Lysosome labeling viruses for autophagy and degradation pathway-related applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Peroxisome labeling viruses for oxidative stress response and metabolic studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Cytoskeleton labeling viruses for cell morphology analysis and structural organization studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Autophagosome labeling of viruses for cellular recycling pathway and autophagy flux studies</li>
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<h2 dir="ltr"> How to Choose an Organelle Labeling Lentivirus</h2>
 Target Organelle Available targeting constructs cover mitochondria, endoplasmic reticulum, nucleus, Golgi apparatus, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; select based on the structure of interest in your experiment. Fluorophore Choice GFP- and RFP-based constructs allow flexibility for multiplexed imaging &mdash; pairing an organelle marker with a second fluorescent reporter (e.g. a protein-of-interest fusion) in the red or green channel respectively. Stable vs. Transient Labeling Needs Lentiviral delivery produces stable, integrated reporter expression maintained across cell divisions &mdash; essential for long-term time-lapse imaging, drug treatment time courses, or generation of reporter cell line banks. Cytotoxicity Considerations Unlike chemical dyes (MitoTracker, LysoTracker, ER-Tracker), which can be cytotoxic at working concentrations and require repeated application, lentiviral organelle markers avoid repeated dye loading and chronic toxicity in long-term experiments. Multiplexing Strategy When combining an organelle marker with other fluorescent reporters, select spectrally distinct fluorophores to avoid channel overlap in fluorescence microscopy or flow cytometry. 
<h2 dir="ltr"> Specifications Context</h2>
 Organelle labeling lentiviral particles sourced from Applied Biological Materials (ABM) are supplied as ready-to-transduce stocks, eliminating the need for in-house viral production. Stable genomic integration enables longitudinal analysis of organelle morphology and dynamics across cell passages, treatments, and differentiation time courses. All lentiviral workflows require institutional biosafety committee (IBC) approval, with BSL-2 containment standard for third-generation systems. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>of MBP to confirm availability for specific organelle targets and fluorophore combinations.

Organelle Labeling Viruses

Important Considerations for Lentiviruses

Peroxisome Labeling Lentivirus (PTS)(GFP)

LVP724 by Applied Biological Materials is a pre-packaged lentivirus (2 × 100 µl) expressing GFP under a peroxisome-targeting sequence (PTS) for stable, specific green fluorescence labeling of peroxisomes in transduced mammalian cells. For live-cell peroxisome dynamics imaging, biogenesis studies, pexophagy assays, and two-color co-localization with RFP organelle markers (LVP722, LVP731). Store at &minus;80°C; single-use aliquots.

Titer	

Peroxisome Labeling Lentivirus (PTS)(RFP)

LVP725 by Applied Biological Materials is a pre-packaged lentivirus (2 × 100 µl) expressing RFP under a peroxisome-targeting sequence (PTS) for stable, specific red fluorescence labeling of peroxisomes in transduced mammalian cells. RFP complement to LVP724 (PTS-GFP). For live-cell peroxisome dynamics imaging, peroxisome biogenesis studies, and two-color co-localization with GFP-labeled organelles. Store at &minus;80°C.

Titer        

pGEX-KG-FLAG-REST

AX0010007 by Applied Biological Materials is a pGEX-KG-based expression plasmid (1.0 µg) encoding human REST/NRSF transcription factor with GST and FLAG dual tags. Use for REST overexpression, anti-FLAG ChIP of REST target genes, protein-protein interaction studies with REST co-repressor complexes, and recombinant GST-REST protein production in bacteria via glutathione affinity purification.