ABM Reagents

<h2 dir="ltr"> What Are Lentiviral Purification &amp; Packaging Kits?</h2>
 Producing high-quality lentiviral stocks is a prerequisite for reproducible transduction experiments. Low-titer or impure virus preparations reduce transduction efficiency, introduce toxicity from residual cell debris, and compromise experimental reproducibility. Purification and packaging kits address every step from plasmid transfection through virus harvest and concentration. Vector production quality depends on efficient purification and packaging workflows. Choose the kit designed for your system.
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<h2 dir="ltr">What you will find:</h2>
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2nd and 3rd Generation Lentivirus Bundles combining transduction enhancers, transfection reagents, packaging mix, titer kits for complete workflows
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2nd and 3rd Generation Packaging System Mix for standardized viral production workflows
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DNAfectin&trade; Plus and Packaging Mix systems for enhanced transfection workflows
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Lentivirus Promoter Blast&trade; Kit for lentiviral expression studies driven by promoters
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SARS-CoV-2 S Protein Pseudotype Lentiviral Packaging Mix for pseudovirus-based research applications
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Ultra-Pure Lentivirus Purification Kit for viral cleanup and concentration
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ViralEntry&trade; Transduction Enhancer for improved transduction
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qPCR Lentivirus Titer Kit &amp; ViralEntry&trade; Bundle for optimized transduction workflows and&nbsp; viral quantification&nbsp;
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<h2 dir="ltr"> How to Choose a Purification &amp; Packaging Kit</h2>
 Concentration Method Available PEG precipitation kits work with a standard benchtop centrifuge, producing 10&ndash;100-fold concentration &mdash; the most accessible method for labs without ultracentrifuges. Ultrafiltration (100 kDa cutoff) offers high recovery (&gt;80%) while removing small molecules and unincorporated VSV-G. Ultracentrifugation provides the highest concentration (up to 1000&times;) but requires specialised equipment. Packaging Plasmid Generation 3rd generation systems (pMDLg/pRRE + pRSV-Rev + pVSV-G) split packaging elements across three plasmids and eliminate Tat for biosafety. 2nd generation systems use a single packaging plasmid + pVSV-G; some transfer vectors with RSV promoters produce lower titers with 3rd generation packaging unless using CMV-promoter variants (pLKO.1C). Starting Volume Scale Ultrafiltration units are compatible with both small-scale (&le;20 mL) and large-scale (up to 1 L) starting volumes using appropriately sized membranes. Plasmid Transfection Ratio For 3rd generation packaging, a 4:2:1:1 ratio of transfer vector:pMDLg/pRRE:pVSV-G:pRSV-Rev has been validated to maximise titer, with 20 &micro;g transfer vector per 15 cm dish as a standard starting point. Titer Assessment Method p24 ELISA measures total viral particle content; functional transduction assays (GFP or antibiotic selection) measure infectious titer; qPCR-based RNA titration measures packaged RNA copies. Functional titer is most relevant for transduction experiments. 
<h2 dir="ltr"> Specifications Context</h2>
 Lentiviral concentration workflows commonly combine PEG precipitation, ultrafiltration, or density-gradient methods to increase viral titer without requiring ultracentrifugation. Ultrafiltration systems using 100 kDa membranes efficiently retain lentiviral particles (approximately 80&ndash;120 nm) while removing smaller contaminants and free proteins. Following concentration, viral stocks are typically aliquoted into single-use volumes and stored at &minus;80 &deg;C because infectivity decreases with repeated freeze&ndash;thaw cycles and prolonged storage above freezing temperatures. Modern 2nd- and 3rd-generation packaging systems separate essential viral functions across multiple plasmids, including a dedicated VSV-G envelope plasmid, to support safer lentiviral production workflows. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. and get reliable lentiviral purification and packaging kits for your lab today.

Purfication & Packaging Kits

DataSheet

MSDS - LV900

MSDS  - G2500

3rd Generation Lentivirus Bundle 3 (LV053-Packaging Mix, G2500-Transfection Reagent, LV900-Titer Kit)

Lenti-Bundle-3 by Applied Biological Materials bundles the 3rd Generation Packaging System Mix (LV053), DNAfectin&trade; Plus Transfection Reagent (G2500), and qPCR Lentivirus Titer Kit (LV900) for complete in-house lentiviral packaging and titer quantification. Ideal for researchers new to lentiviral production and experienced users seeking a cost-effective combination.

Unit Quantity

Applications

Shipping Condition

MSDS - G2500

MSDS - G515

3rd Generation Lentivirus Bundle 4 (LV053-Packaging Mix, G2500-Transfection Reagent, LV900-Titer Kit, G515-Transduction Enhancer)

Lenti-Bundle-4 by Applied Biological Materials is a complete 3rd-generation lentiviral workflow bundle: LV053 (3rd-gen packaging mix, 200 µL) + G2500 (DNAfectin™ Plus transfection reagent) + LV900 (qPCR titer kit, 100 rxn) + G515 (ViralEntry™ transduction enhancer 100X, up to 10× boost). For complete lentiviral packaging, titration, and transduction optimization in one purchase.

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Enhanced Lentivirus Safety Features Replication Incompetency

Suggested MOI for Common Cancer Cell Lines

Lentiviral System FAQ

3rd Generation Packaging System Mix

LV053 by Applied Biological Materials is a 200 µL 3rd Generation Packaging System Mix (pLenti-P3A + pLenti-P3B + pLenti-P3C) for maximum-biosafety 4-plasmid lentiviral production. Rev on independent vector; TAT-independent; VSV-G pseudotyped. Compatible with 3rd-gen vectors (chimeric 5′ LTR) only. Store at −20°C or below.

Additional Information

Note

<h2 dir="ltr">What Are AAV Control &amp; Reporter Constructs?</h2>
 Establishing reliable experimental controls is critical in AAV research because transduction efficiency varies substantially by serotype, cell type, multiplicity of infection (MOI), and batch-to-batch variation in viral preparation quality. Matched AAV control and reporter constructs provide the reference point for interpreting experimental results.
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Promoter-Specific Blank Vectors used for baseline control, utilize neuron-specific hSyn promoters and high-purity plasmids&nbsp;
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Serotype-Matched Blank Viruses for precise tissue-targeting validation. Available pre-packaged in serotypes 1 through 11
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Bioluminescent Reporter Systems for sensitive measurement of viral delivery.&nbsp; And gene expression featuring luciferase and SV40-luc markers
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Fluorescent Control Constructs carry circ-GFP, GFP, and CBh-gcGFP, for real-time transduction tracking.
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Circular RNA Control Plasmids for advanced non-coding RNA research.&nbsp;
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Cloning-Ready Expression Backbones for selected gene insertion and viral packaging workflows using ampicillin-resistant blank vectors
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<h2 dir="ltr"> How to Choose AAV Control &amp; Reporter Constructs </h2>
 Serotype and Backbone Matching The control construct should use the same AAV serotype, ITR configuration, and promoter as the experimental construct, ensuring transduction efficiency differences reflect the transgene rather than vector or serotype variation. Fluorescent Reporter Choice GFP and mCherry reporters allow confirmation of transduction by fluorescence microscopy or flow cytometry, with mCherry offering a red-channel option for multiplexed experiments. Luciferase and Dual-Reporter Options Firefly luciferase provides quantitative bioluminescent transduction confirmation; dual reporter systems (e.g., fluorescent + luciferase) allow both visual and quantitative readouts from the same construct. Empty Vector Baseline An empty AAV vector &mdash; same ITRs, serotype, and promoter, no transgene &mdash; establishes the baseline effect of AAV capsid delivery and innate immune activation, independent of the gene of interest. Batch-to-Batch Variation Controls Because AAV preparation quality can vary between batches, including a transduction-efficiency control (e.g., GFP reporter) alongside each new batch of experimental AAV helps confirm comparable functional titer. 
<h2 dir="ltr"> Specifications Context</h2>
 AAV transduction can activate innate immune sensing pathways and, at high vector doses, induce cellular stress responses independently of the transgene. A matched empty vector or reporter control sharing the same serotype and promoter as the experimental construct helps distinguish vector-related effects from genuine transgene-specific phenotypes. Because AAV infectivity can vary between preparations, reporter-based transduction assessments are commonly used to verify functional performance before experimental studies. For in vivo applications, pilot reporter studies can help confirm tissue targeting and delivery efficiency before larger cohorts are committed. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to get high-quality control/reporter vectors for your lab.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

AAV Blank Control Vector (CMV) (Amp)

AAV Blank Control Vector (CMV)(Amp) (AAV001a) by Applied Biological Materials is a 1.0 µg insert-empty, reporter-free AAV plasmid with CMV promoter and ampicillin resistance, for use as a reporter-free negative control in CMV-promoter AAV expression experiments. Use when no reporter signal is desired in the control arm. Store at –20°C or below.

Vector Map

Guarantee

AAV Blank Control Vector (CMV) (CBH-gcGFP) (Amp)

AAV Blank Control Vector (CMV)(CBh-gcGFP)(Amp) (AAV016a) by Applied Biological Materials is a 1.0 µg insert-empty AAV plasmid with CMV promoter backbone, CBh-driven gcGFP reporter, and Amp selection, for use as a negative control in CMV-promoter AAV experiments with fluorescence-based transduction monitoring. Store at –20°C or below.

Quantity

List of Markers

AAV Blank Control Vector (CMV) (GFP) (Amp)

AAV Blank Control Vector (CMV)(GFP)(Amp) (AAV003a) by Applied Biological Materials is a 1.0 µg insert-empty AAV plasmid with CMV promoter, GFP reporter, and ampicillin resistance, for use as a negative control in CMV-promoter AAV expression experiments with GFP-based transduction monitoring. Store at –20°C or below.

AAV Blank Control Vector (CMV) (Luc) (Amp)

AAV Blank Control Vector (CMV) (Luc) (Amp) (AAV002a) by Applied Biological Materials is a 1.0 µg insert-empty AAV plasmid with CMV promoter, Luciferase reporter, and ampicillin resistance — for use as a negative control in CMV-promoter AAV expression experiments with bioluminescence-based transfection monitoring. Store at –20°C or below.

AAV Blank Control Vector (hSync) (Amp)

AAV Blank Control Vector (hSyn)(Amp) (AAV022a) by Applied Biological Materials is a 1.0 µg insert-empty AAV plasmid with neuron-specific hSyn promoter and ampicillin resistance, for use as a matched negative control in hSyn-promoter AAV neuron-specific expression experiments. Store at –20°C or below.

AAV Blank Control Virus (CMV)  (Amp) (Serotype 2)

AAV Blank Control Virus (CMV)(Amp)(Serotype 2) (AAVP002a) by Applied Biological Materials is a packaged AAV2 blank control virus at 10¹¹ GC/mL, supplied as 5 × 100 µl, for use as a serotype-matched, capsid-equivalent negative control in CMV-promoter AAV2 gene expression experiments. Shipped on dry ice; store at –80°C.

Titer

Storage Condition

AAV Blank Control Virus (CMV) (Amp) (Serotype 1)

AAV Blank Control Virus (CMV)(Amp)(Serotype 1) (AAVP001a) by Applied Biological Materials is a packaged AAV1 blank control virus at 10¹¹ GC/mL, supplied as 5 × 100 µl, for use as a serotype-matched, capsid-equivalent negative control in CMV-promoter AAV1 gene expression and muscle/neuronal gene delivery experiments. Shipped on dry ice; store at –80°C.

AAV Blank Control Virus (CMV) (Amp) (Serotype 3)

AAV Blank Control Virus (CMV)(Amp)(Serotype 3) (AAVP003a) by Applied Biological Materials is a packaged AAV3 blank control virus at 10¹¹ GC/mL, reporter-free, supplied as 5 × 100 µl for use as a serotype 3-matched negative control in CMV-promoter AAV3 gene expression experiments. Shipped on dry ice; store at –80°C.

AAV Blank Control Virus (CMV) (Amp)(Serotype 11)

AAV Blank Control Virus (CMV) (Amp)(Serotype 11) (AAVP177a) by Applied Biological Materials is a packaged AAV11 blank control virus at 10¹¹ GC/mL, reporter-free, supplied as 5 × 100 µl for use as a serotype 11-matched negative control in CMV-promoter AAV11 gene expression experiments. Shipped on dry ice; store at –80°C.