In this blog, we will take a look at different types of serological pipettes and their uses:
Serological Pipettes – A Guide To Their Types & Uses
● Uses Of Serological Pipettes
- Used for mixing together suspensions
- Used to combine chemical solutions and reagents.
- Used for the transference of cells for analysis and testing.
- Used for the layering of reagents.
The main design of serological pipettes is dependent on either gravitational pressure or air. That is why they are considered to be blow-out pipettes. The rubber bulb on the pipette is squeezed to let the serum or fluid inside the pipette out.
Blow-out pipettes can be identified by the double rings they have on them.
● Different Kinds Of Serological Pipettes
There are many different kinds of serological pipettes. They are usually made of a high-quality polymer or plastic or, in many cases, glass. These materials are used for sterilization and safety purposes. Serological pipettes are also designed to be single-use.
1. Open-End Pipettes
The rear end of open-end pipettes is exposed. And therefore, they are perfect for measuring liquids with a high viscosity. Because they are open-ended, the release rate of the pipette is fast. This allows thick liquids like paint and oil to flow freely through the pipettes.
And these kinds of pipettes also have a focus on sterilization too. They do this by having a fiber filter plug included. This reduces the chances of the fluid being contaminated by foreign particulates. They come tightly packed in individual plastics.
2. Bacteriological Pipettes
When dealing with substances with bacteria, such as milk or other dairy products, bacteriological products are used because they are designed specifically for that purpose. This kind of pipette is also known as a milk pipette, and it is available in various measurements. It is usually made of polystyrene. These pipettes are highly sensitive, non-pyrogenic, and single-use. They are sterilized using gamma radiation and packed tightly in plastic film individually to protect them from any damage or contaminations. They also have a fiber filter that prevents contamination of the liquid or fluid contained within them.
3. Aspirating Pipettes
When it comes to pure fluid transfer, the best option is an aspirating pipette. They are fully transparent and don't have any graduations or marking on them. They are also single-use, non-pyrogenic, and made from polystyrene. They are specifically designed for mixing fluids in a vacuum or in a pipette aspiration process.
These pipettes come wrapped in thermoformed high-quality plastic, and they are also wrapped individually to keep any chances of contamination as low as possible. And like bacteriological pipettes, they are also sterilized using gamma radiation.
● Dispensation & Aspiration Of Fluids Using Serological Pipettes
The basic way of using any pipette is just using the bulb or pump attached to the rear end of the pipette. The difference between a bulb and a pump is the accuracy is the amount of fluid you take. A bulb, due to its primitive nature, will drag different amounts of fluid depending upon the pressure you put on it, and you would have to reduce or redraw fluid to get the exact amount you want.
In contrast, a pump would take the exact amount of fluid that you want, which is much quicker and more convenient.
In Conclusion
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