dNTP Mix: Premixed Equimolar Nucleotide Solutions for PCR
dNTP Mix is a premixed, equimolar solution of dATP, dCTP, dGTP, and dTTP - commonly supplied at 10 mM each (from a 100 mM total stock) or other standard working concentrations - ready for direct addition to PCR, qPCR, and cDNA synthesis reactions. The premix eliminates the need for a manual equimolar combination of four separate nucleotide tubes.
Lab managers running high-throughput PCR benefit from the consistency and reduced pipetting steps of a factory-balanced premix. Contact customerservice@mbpinc.net to request a quote today!
dNTPs Mix
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What is a dNTP Mix?
A dNTP Mix is a premixed solution containing all four deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - combined in equimolar amounts at a standard working concentration, commonly 10 mM each (40 mM total) when prepared from 100 mM individual stocks. The premix is ready for direct addition to a PCR, qPCR, or cDNA synthesis reaction without further dilution or balancing. Choose a premixed dNTP Mix for routine PCR setup where convenience and lot-to-lot consistency outweigh the need for custom nucleotide ratios.
What you will find:
- High-purity nucleotide mixes for PCR and DNA synthesis applications, including PureStrand™ Ultra-Pure dNTP Mix (Azura)
- Standard 10 mM each dNTP formulations suitable for routine PCR workflows, including dNTP Mix (10 mM each) formats
- Higher-concentration nucleotide solutions, such as Accuris 40 mM dNTP Mix for optimized reaction setup flexibility
- Ready-to-use balanced dNTP blends supporting DNA polymerase activity in PCR and related enzymatic amplification reactions
- Nucleotide triphosphate formulations, including NTP Mix (G945), for broader enzymatic and molecular biology applications
How to Choose a dNTP Mix
Concentration Format
Common premix formats include 10 mM each (40 mM total, a 50x working stock relative to the 0.2 mM final concentration) and 2.5 mM each (10 mM total, a 10x working stock). Select the format that aligns with your typical reaction volume and pipetting precision - more dilute stocks reduce pipetting error for small-volume reactions.
Equimolar Accuracy
Because all four nucleotides are pre-combined at equal concentrations, a premixed dNTP Mix removes the risk of manual equimolar-mixing error that can occur when combining four separate 100 mM stocks - unbalanced ratios increase misincorporation frequency during polymerisation.
Throughput Considerations
For high-throughput PCR setups across multi-well plates, a premixed dNTP Mix reduces the number of individual pipetting steps per reaction compared to adding four separate dNTP stocks, improving both speed and reproducibility.
Compatibility with Master Mixes
Many 2x PCR master mixes already include dNTPs at optimised concentrations - a separate dNTP Mix is primarily needed for labs assembling reactions from individual components (enzyme, buffer, dNTPs, primers) rather than using a pre-formulated master mix.
Application Range
A standard equimolar dNTP Mix supports PCR, qPCR, and cDNA synthesis (as substrate for reverse transcriptase). Confirm the concentration and purity grade align with the specific requirements of high-fidelity or long-range PCR, if applicable.
Specifications Context
To prepare a standard PCR reaction at the recommended 0.2 mM final concentration of each dNTP using a 10 mM-each premix, 1 µL of the premix is added per 50 µL reaction. Equal nucleotide concentrations in the premix are essential because unbalanced dNTP ratios increase the frequency of misincorporation during DNA synthesis - a factory-balanced premix removes this variable. dNTP Mix is typically supplied in water at pH 7.5, stable at −20 °C with documented multiple freeze-thaw tolerance; thawing and mixing thoroughly before use is recommended, especially for higher-concentration formats.
Contact the expert team at MBP today and get high-purity dNTP mixes tailored for your laboratory workflows.
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