dNTP Set

What is a dNTP Set?

A dNTP Set is a complete set of individual, highly pure deoxynucleotide triphosphates - dATP, dCTP, dGTP, and dTTP - each supplied as a separate 100 mM solution in water. Each nucleotide can be diluted and mixed with any of the other three to the desired final concentration or ratio, giving researchers full control over custom PCR, qPCR, or labelling mix preparation. Choose a dNTP Set when you need to prepare custom-concentration mixes, run multiple applications with different nucleotide requirements, or want individual stocks for non-equimolar formulations.

What you will find:

• Pre-mixed deoxynucleotide triphosphate (dNTP) sets for PCR and DNA synthesis applications, including PureStrand™ dNTP Set (Azura)
• High-concentration nucleotide formulations such as Accuris 100mM dNTP Set for flexible reaction setup and optimization
• Standard dNTP set formats suitable for routine PCR workflows, including dNTP Set (G050)
• Nucleotide triphosphate formulations for enzymatic and molecular biology applications, including NTP Set (G946)
• Ready-to-use balanced nucleotide sets supporting DNA polymerase activity in PCR and related amplification reactions

How to Choose a dNTP Set

Custom Mix Preparation
A dNTP Set's primary advantage is flexibility - preparing a working mix at any desired total concentration and ratio, including non-standard formulations for specific applications such as nucleotide analog substitution (e.g., dUTP in place of dTTP for anti-carryover-contamination strategies).

Multiple Application Support
Labs running PCR, qPCR, cDNA synthesis, and DNA labelling from the same stock benefit from a single dNTP Set, since each application may call for slightly different concentrations or ratios prepared from the same individual 100 mM tubes.

Stock Concentration
100 mM is the standard individual stock concentration for dNTP Sets, providing substantial dilution headroom - a 100 mM stock can be diluted 1000-fold to reach a 0.1 mM working concentration, or combined at lower dilution ratios for higher-concentration premixes.

Equimolar Mixing Discipline
When preparing a standard equimolar mix from a dNTP Set, equal volumes of each 100 mM nucleotide are combined with water to the desired total concentration - for example, equal-volume mixing of all four 100 mM stocks yields a 25 mM-each premix at 100 mM total.

Purity and Lot Testing
PCR-grade dNTP Sets are typically tested at greater than 99% purity by HPLC, with concentration verified by optical density spectrometry and absence of human/E. coli DNA confirmed by qPCR - important documentation for labs requiring lot-specific quality records.

Specifications Context

To prepare a standard equimolar working mix from a dNTP Set, combining equal volumes of each 100 mM individual stock yields a mix at 25 mM of each nucleotide (100 mM total); this can be further diluted to common working stocks such as 10 mM each (a 50x stock relative to the 0.2 mM PCR final concentration) or 2.5 mM each (a 10x stock). The performance of each lot is typically functionally tested in a PCR requiring all four nucleotides, with concentration verified by optical density spectrometry and purity confirmed by HPLC. dNTP Sets are stable at −20 °C with documented multiple freeze-thaw tolerance; thawing and mixing each individual tube thoroughly before use is recommended.

Contact the expert team at MBP today and get reliable dNTP sets for your next successful experiment.