Buffers

Research-grade buffers for accurate immunoprecipitation, chromatin preparation, washing, and DNA recovery in epigenetics ChIP workflows.

MBP ChIP buffers are designed to ensure reliability in all the steps of ChIP workflows, including fragmentation of chromatin, nuclear isolation, binding of antibody, washing, elution, and DNA purification. These buffers are widely used in transcription factor assays, histone modification analysis, gene regulation, PCR,  functional genomics, and epigenetics research workflows. They help improve assay specificity, maintain high-integrity chromatin, and ensure scalable downstream analysis.

What you will find:

• MN Digestion Buffer used in MNase-based workflows for enzymatic fragmentation of  chromatin 
• Chromatin Shearing Buffer using sonification for chromatin disruption and preparation
• 5X MN Stop Buffer for the termination of enzymatic digestion reactions
• Chromatin Dilution Buffer used in ChIP assays to provide optimized conditions for antibody binding 
• Chromatin Wash Buffer I, II, and III for background reduction and step-by-step non-specific binding removal 
• ChIP DNA Binding Buffer for purification and capture of immunoprecipitated DNA
• 5X Chromatin Elution Buffer for protein–DNA complexes recovery
• 5M NaCl for enhancing elution and chromatin stabilization
• Nuclei Prep Buffer used before chromatin extraction for superior nuclear isolation

MBP’s ChIP buffers are formulated to deliver consistent immunoprecipitation performance, reliable chromatin isolation, and optimized antibody-binding workflows across epigenetic workflows. These essential reagents enhance downstream DNA recovery and reduce background interference in transcriptional regulation and epigenetics studies. 

Contact the expert team at MBP and request a quote today to get high-quality ChIP buffers for your lab.