DNA/Cloning Services Products

What Are DNA Cloning Services Products?

DNA cloning products are the vectors, enzymes, and constructs that support molecular cloning — isolating, replicating, expressing, and manipulating DNA sequences for functional study, protein production, and gene delivery. MBP's category spans the full workflow, from restriction-ligation and assembly cloning components through to complete ready-to-use lentiviral ORF constructs for direct transduction. Successful construct development begins with reliable cloning tools and services. Select the resources that match your workflow requirements.

What you will find:

• AAV Vectors: for stable expression and rapid gene delivery in vitro and in vivo. Applications include CRISPR studies, functional gene expression, and gene therapy research.

• Lentivirus Vectors: for long-term expression and stable gene integration in both proliferating and quiescent cells. Used in stable cell line development, gene editing, and vaccine production.

• Adenovirus Vectors: High efficiency gene expression for diverse cell types. Applied in oncolytic viral therapy, vaccine development, and functional screening assays.

• Protein Expression Vectors: for production and expression of recombinant protein in target host systems for diverse research applications.

• Retrovirus Vectors: for long-term gene expression and stable genomic modification, commonly used in gene therapy and cell engineering.

• Specialized Cloning Vectors / Viral Systems: for specialized gene delivery applications, advanced cloning techniques, and optimized model construction.

How to Choose DNA Cloning Products

Cloning Method
Restriction enzyme ligation, Gibson Assembly, Golden Gate, and Gateway recombination cloning are all supported. Gibson and Golden Gate suit multi-fragment assembly without restriction site constraints; Gateway enables rapid parallel expression testing across host systems.

Construct Format
Lentiviral ORF constructs — human, mouse, and rat gene libraries — are available as plasmid DNA for in-house packaging or pre-packaged as recombinant lentivirus particles, in tagged (DDK/FLAG, GFP, RFP) and untagged formats.

Bacterial Strain Requirements
Lentiviral plasmids contain LTRs prone to recombination in standard E. coli strains (DH5α, TOP10). Recombination-resistant strains such as Stbl3 are strongly recommended for propagation, transformation, and miniprep/midiprep preparation.

Promoter Selection
The EF1α promoter provides more stable long-term transgene expression in stable cell lines compared to CMV, which tends to undergo silencing over time in many mammalian cell types — studies show sustained GFP expression above 80% for 30+ days with EF1α-driven constructs.

Plasmid Purity for Transfection
Plasmid DNA purified using silica-column methods and verified for endotoxin levels is recommended for mammalian transfection, particularly into sensitive primary cells and stem cell lines.

Specifications Context

Applied Biological Materials (ABM) is a featured partner brand for lentiviral cloning products at MBP, offering human, mouse, and rat ORFs cloned into lentiviral vectors — including tagged and untagged formats across 35,000+ genes — for over-expression and knockdown research. DNA cloning reagents are consumed at high volumes in core facility environments; MBP's institutional account structure supports standing orders, bulk pricing, and priority fulfilment. Plasmid DNA and many cloning enzymes require refrigerated (4 °C) or frozen (−20 °C/−80 °C) shipping depending on the product. Confirming storage requirements before placing an order helps labs schedule receipt around staff availability, particularly for temperature-sensitive lentiviral plasmid shipments arriving outside normal working hours. Product availability reflects MBP's catalogue as of mid-2026.

Contact the expert team of MBP and get reliable DNA and cloning services products for your lab today.