Specialized Cloning Vectors & Viral Delivery Tools

MBP Inc. supplies specialized cloning vectors and viral tools for advanced cell biology applications beyond standard over-expression and knockdown systems — including organelle-targeted fluorescent labeling viral vectors, bicistronic and multicistronic expression systems, Cre-based conditional expression and recombination tools, and inducible constructs for research applications across the USA and Canada.

MBP provides specialized cloning vectors and viral systems for advanced molecular biology and gene-delivery workflows. Request a quote by contacting customerservice@mbpinc.net

Specialized Cloning Vectors/ Virus

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What Are Specialized Cloning Vectors & Viral Tools?

Standard lentiviral and retroviral expression vectors meet the needs of most over-expression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions — subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.

What you will find:

Organelle Labeling Viruses: for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems

How to Choose Specialized Cloning Vectors

Subcellular Targeting Needs
Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton — providing a stable alternative to chemical dyes for live-cell imaging.

Multi-Gene Co-Expression
Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector.

Conditional Expression Requirements
Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies.

Temporal Control Needs
Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing.

Delivery Format
Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models.

Specifications Context

Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP’s catalogue as of mid-2026.

Contact the MBP team today and get specialized cloning vectors for your lab.

FAQ

MBP supplies organelle-targeted fluorescent labeling lentiviral particles, bicistronic vectors (IRES and 2A peptide), conditional DIO/FLEX vectors for Cre-dependent expression, inducible Tet-On/Off systems, secretion signal vectors for secreted protein production, and fusion protein vectors -- sourced primarily through the ABM specialised vector portfolio.

A bicistronic vector co-expresses two proteins from a single mRNA using either an IRES element (for cap-independent translation of the second ORF at lower levels) or a 2A self-cleaving peptide (for near-equimolar production of both proteins by ribosomal skipping). These are used when a gene of interest must be co-expressed alongside a selection marker or reporter from a single integrated lentiviral construct.

IRES-based vectors produce the downstream protein at 10-30% of the upstream protein level, as cap-independent translation is inherently less efficient. 2A peptide sequences (T2A, P2A, E2A, F2A) produce two separate proteins at approximately equimolar ratios via ribosomal skipping during translation. 2A systems are preferred when equal co-expression of both proteins is required.

Yes. MBP can coordinate custom vector construction through manufacturer partnerships including ABM's Custom Cloning Service, which supports design, cloning, and packaging of custom lentiviral constructs at research-grade titers up to 1010 IU/ml. Contact MBP with your construct requirements for a custom quote.

Yes. Specialised transfer vectors using standard lentiviral backbone elements (LTRs, Psi packaging signal, RRE) are compatible with 2nd and 3rd generation packaging plasmid systems used for standard lentiviral vector production. Always verify that the 5' LTR configuration (Tat-dependent vs. chimeric CMV-driven) matches the packaging system generation.

Tet-On vectors activate transgene expression when doxycycline is added to culture medium; Tet-Off vectors drive expression in the absence of doxycycline and silence it upon doxycycline addition. These are used for experiments requiring temporal control of gene expression -- particularly for studying essential genes, toxic proteins, or dose-response relationships where constitutive expression would compromise experimental design.

A secretion signal vector includes an N-terminal signal peptide that directs the expressed protein into the ER lumen for glycosylation and secretion via the Golgi apparatus. These vectors are used for producing secreted proteins, recombinant antibody fragments, cytokines, and extracellular domain constructs that must be exported from cells for downstream purification or activity assays.

Yes. Cre-inducible DIO (Double-floxed Inverted Open reading frame) and FLEX vectors -- used extensively in neuroscience with Cre-driver transgenic mouse lines for cell-type-specific gene expression -- are part of MBP's specialised vector range. These vectors are silent until Cre-mediated ORF inversion activates expression permanently in Cre-expressing cells.

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