Cryogenic storage vials are tiny, capped, cylindrical containers cautiously designed for storing and preserving samples to survive temperatures as low as -196℃. Cryogenic storage vials can hold various cell types, varying from diagnosing stem cells, microorganisms, and primary cells to forming cell lines. They come in multiple forms and having the proper style that fulfils all your wants will confirm that you maintain sample integrity without paying extra.
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Properties of Cryogenic Vial to Study
It is vital to have a know-how of the properties of the Cryogenic Vials before purchase which is as follows:-
External vs. Internal Threads
Both threads differ significantly, and people need to consider the differences before making any personal choices during purchase. Many laboratories often want to diminish tube storage space and go for an internal thread to let a better fit into freezer boxes.
Despite this, the externally threaded option is the better choice for you which carries a lower contamination risk. The design makes it harder for anything other than the sample to enter the vial. Externally threaded vials are generally chosen for genomic applications, but both choices are suitable for biobanking and other high throughput applications.
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Storage Volume
Cryogenic vials are formed in multiple sizes, but mainly they range between a capacity of 1 mL and 5 mL. Make sure your cryovial is not overfilled, and extra room exists in case the sample swells while freezing. Laboratories pick 1 mL vials when keeping samples of 0.5 mL cells suspended in cryoprotectant and 2.0 mL vials for 1.0 mL of sample. Another Tip to avoid overfilling is to use cryovials with graduated markings to prevent any swelling which could cause leaking or cracking.
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Screw Cap vs. Flip Top
The top you choose depends on the usage of liquid phase nitrogen or not. You will need screw-capped cryovials for the nitrogen liquid phase, which confirms that they can't pop open mistakenly due to temperature changes or mishandling. Screw caps allow for easier recovery from cryogenic boxes and more effective storage.
However, if you are not using liquid stage nitrogen and want a more convenient top that is easily opened, then a flip top is a good choice. The flip lid is handy in higher data operations and batch processes.
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Seal Security
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Glass vs. Plastic
Most laboratories use plastic, generally polypropylene, for safety and convenience in place of heat-sealable glass ampules. Glass ampules are now considered an old-fashioned choice. During sealing, invisible pinhole leaks may occur on glass ampules, which, when melted after storage in liquid nitrogen, may cause them to explode. They are also unsuitable for modern labeling procedures, which ensure sample traceability.
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Self-Standing vs. Rounded Bottoms
Cryogenic vials are available as self-standing with rounded bottoms or star-shaped bottoms. Choose self-standing to place your vials on a surface.
Storage Temperature
There are multiple storage approaches for the cryogenic storage of samples. Each works at a specific temperature.
- Nitrogen vapor freezers - Ranges -20°C to -150°C
- Vapor phase LN2 - handle a specific temperature range between -135°C and -190°C depending on the model.
- Liquid phase LN2 - keeps a temperature of -196℃
The type of cells being stored and a researcher's preferred storage method will determine your laboratory's three choices. However, not all tubes or designs will be suitable or safe. Using a cryogenic vial not ideal for your temperature could cause the vessel to crack during thawing or storage. Cautiously check the manufacturers' recommendations on proper use as some cryogenic vials are suitable for temperatures as low as -175°C, some -150°C, and others just 80°C.
Conclusion
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