Square Well Deep Well Plates for High-Throughput Sample Processing and Storage
Square well deep well plates are 96-well polypropylene microplates with square-geometry wells holding 1.1 mL, 2.0 mL, or 2.2 mL per well, providing maximum working volume within the SBS/ANSI footprint. Available in V-bottom (complete aspiration, pellet recovery) and U-bottom (mixing, culture) configurations. Certified DNase-free, RNase-free, and PCR-inhibitor-free; rated to 4,000–6,000 × g. Compatible with Hamilton, Tecan, and Beckman liquid handlers. MBP ships to labs across the United States, Canada, and internationally with PO ordering at mbpinc.net.
Need help selecting the right square well deep well plates for your workflow? Contact customerservice@mbpinc.net for product recommendations, pricing, and procurement support.
Square Well Deep Well Plates
What Are Square Well Deep Well Plates?
Square well deep well plates are polypropylene microplates designed with square-shaped wells instead of traditional round wells. This design maximizes liquid capacity within the standard SBS/ANSI microplate footprint, making them ideal for high-throughput laboratory workflows that require larger sample volumes. Commonly available in 96-well formats with capacities ranging from 1.1 mL to 2.2 mL per well, these plates are widely used for nucleic acid extraction, protein precipitation, compound library storage, sample preparation, and automated liquid handling.
The square-well geometry increases usable volume while maintaining compatibility with automated platforms from Hamilton, Tecan, Beckman Coulter, and Agilent. Manufactured from virgin polypropylene, square well plates offer excellent chemical resistance to DMSO, ethanol, acetonitrile, and extraction buffers, while minimizing sample binding and contamination risks.
Product Range:
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96-square well storage plates for sample management.
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2.2ml deep well plates for large-volume handling.
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V-bottom square well plates for maximum sample recovery.
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DNA-free deep well microplates for molecular applications.
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Medical-grade polypropylene plates for chemical durability.
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Automation-ready square well formats for robotic integration.
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Deep well plates for bead beating and high-impact use.
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Chemical-resistant 96-well plates for harsh reagents.
How to Choose Square Well Deep Well Plates
Well Volume
The most common formats are 2.0 mL and 2.2 mL square well plates, which provide sufficient volume for nucleic acid extraction, protein precipitation, and compound storage workflows. Lower-profile 1.1 mL plates are useful when deck height limitations or freezer space optimization are important considerations.
Bottom Geometry
V-bottom wells concentrate liquid and pellets into a single point, enabling more complete aspiration and improved recovery during centrifugation-based workflows. U-bottom wells promote better mixing and resuspension, making them suitable for microbial culture applications and serial dilutions.
Centrifugation Requirements
Most square well polypropylene plates are compatible with centrifugation speeds between 4,000 × g and 6,000 × g. For demanding extraction protocols, reinforced plate designs provide additional structural stability during high-speed centrifugation.
Automation Compatibility
Select plates that comply with ANSI/SLAS standards and include molded alphanumeric indexing for sample tracking. Features such as stacking lugs and raised well rims improve robotic handling and sealing performance.
Certification Standards
For molecular biology applications, choose plates manufactured from virgin polypropylene and certified DNase-free, RNase-free, DNA-free, and PCR inhibitor-free. Sterile options are recommended for sensitive workflows and regulated environments.
Common Square Well Deep Well Plate Formats
2.2 mL Square V-Bottom Plates
These are widely used for protein precipitation, liquid-liquid extraction, and workflows requiring maximum liquid recovery.
2.0 mL Square V-Bottom Plates
A standard choice for nucleic acid extraction, sample storage, and automated liquid handling applications.
1.1 mL Square V-Bottom Plates
Ideal for laboratories seeking reduced plate height while maintaining automation compatibility.
2.2 mL Square U-Bottom Plates
Suitable for microbial culture, sample mixing, and applications involving viscous liquids.
Specifications and Applications
Square well deep well plates are typically manufactured from high-purity polypropylene and operate across a wide temperature range, often from approximately –86°C to +125°C. Most plates conform to ANSI/SLAS microplate dimensions (127.76 × 85.48 mm), ensuring compatibility with automated liquid handlers, centrifuges, incubators, and storage systems.
Today, 2.0 mL and 2.2 mL square well V-bottom plates remain the preferred format in pharmaceutical research, clinical diagnostics, genomics laboratories, and biobanking facilities due to their high volume capacity, efficient liquid recovery, and seamless integration into automated workflows.
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