Immortalized Cells

Thawing Protocol

Cell Line Catalog (Volume 2)

Subculturing Protocol

Freezing Protocol

Human Dermal Fibroblasts-fetal Feeder Cells

T2015-1 Human Dermal Fibroblasts &ndash; Fetal Feeder Cells are fetal human dermal fibroblasts providing a human-origin feeder layer for stem and progenitor cell culture in xeno-free or reduced-xeno workflows. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

Subculture Protocol

T2001 Mouse Embryonic Fibroblast Feeder Cells

T2001 Mouse Embryonic Fibroblast Feeder Cells are primary fetal MEF cells — the gold standard feeder layer for ESC and iPSC culture. Inactivate with mitomycin C or irradiation before use. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T2003 Immortalized Mouse Embryonic Fibroblast Feeder Cells

T2003 Immortalized MEF Feeder Cells are an immortalized mouse embryonic fibroblast line providing a renewable, senescence-free source of feeder cells for ESC/iPSC culture; user-inactivate before feeder use. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T2004 Immortalized Human Dermal Fibroblast-fetal Feeder Cells

T2004 Immortalized Human Dermal Fibroblast &ndash; Fetal Feeder Cells are an immortalized fetal human dermal fibroblast line providing a renewable, senescence-free human-origin feeder layer for xeno-free ESC/iPSC workflows; user-inactivate before feeder use. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2009 MITC Treated Immortalized Mouse Embryonic Fibroblast Feeder Cells

T2009 MITC Treated Immortalized MEF Feeder Cells are mitomycin C-inactivated immortalized mouse embryonic fibroblasts providing a consistent, senescence-free feeder layer for ES and iPSC culture. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; BSL-2; RUO. MTA required.

T2010 CB-iPSC

T2010 CB-iPSC are human induced pluripotent stem cells derived from cord blood, offering high reprogramming efficiency and reduced somatic mutation load for hematopoietic differentiation, disease modeling, and regenerative medicine research. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2011 CB-iPSC-2

T2011 CB-iPSC-2 is a second clonal line of human cord blood-derived iPSC, providing an independent clone for experimental replication and clonal variation control alongside T2010 CB-iPSC. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2012 CB-iPSC-3

T2012 CB-iPSC-3 is the third clonal cord blood-derived human iPSC line, enabling multi-clone experimental designs to control for clonal variation in disease modeling, differentiation, and drug screening. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2013 CB-iPSC-4

T2013 CB-iPSC-4 is the fourth clonal cord blood-derived human iPSC line, enabling multi-clone experimental designs to control for clonal variation in disease modeling, differentiation, and drug screening. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2014 CB-iPSC-5

T2014 CB-iPSC-5 is the fifth clonal cord blood-derived human iPSC line, enabling multi-clone experimental designs to control for clonal variation alongside T2010&ndash;T2013 and T2015. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2015 CB-iPSC-6

T2015 CB-iPSC-6 is the sixth clonal cord blood-derived human iPSC line, enabling multi-clone experimental designs to control for clonal variation in disease modeling, differentiation, and drug screening. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.