Brands

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

MSDS

DNA Recovery Buffer (9 ml)

DNA Recovery Buffer R2050-5-9 by Zymo Research — 9 ml buffer for use in the Quick-DNA/RNA Blood Tube Kit (Cat. R1151), removing contaminants from DNA and RNA extracted from whole blood preserved with DNA/RNA Shield™. Sold separately for buffer component replenishment. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

DNA/RNA Buffer HT (100 ml)

R2150-1-100 by Zymo Research is the DNA/RNA Buffer HT (100 ml), a one-step lysis and binding reagent for high-throughput simultaneous DNA and RNA extraction using the Quick-DNA/RNA HT magnetic bead system. Compatible with 96-well and 24-well formats; eluates ready for NGS and RT-qPCR. Requires user addition of beta-mercaptoethanol before use.

Manufacturer

Shipping Condition

Storage Condition

<h2 data-section-id="1ebaq4d" data-start="165" data-end="199">What are transfection reagents?</h2>
&nbsp;
Transfection reagents enable the delivery of exogenous nucleic acids &mdash; plasmid DNA, mRNA, siRNA/shRNA, and CRISPR/Cas9 ribonucleoprotein or plasmid components &mdash; into cultured cells for applications spanning gene expression studies, gene silencing, CRISPR-based gene editing, recombinant protein production, and stable cell line development. Delivery methods fall into three categories: chemical methods (cationic lipid/lipofection reagents, polymeric agents like polyethyleneimine/PEI, and calcium phosphate co-precipitation), physical methods (electroporation and biolistic particle delivery), and viral methods (transduction via lentiviral, adenoviral, or AAV vectors, the only category where "transduction" is the technically correct term). Each category includes multiple specific reagents with different optimal nucleic acid types, cell type compatibility, and transient-versus-stable expression outcomes.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" role="presentation">DNAfectin&trade; Plus Transfection Reagent: innovative lipid-based reagent for rapid transfection in common adherent cell lines; fewer handling steps, and works in complete growth media</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" role="presentation">Susfectin&trade; Transfection Reagent: polymer-based reagents formulated for suspension cultures, providing highly efficient nucleic acid delivery&nbsp;</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" role="presentation">Transfection Reagent: designed for faster and more reliable concentration of lentiviral particles from cell culture supernatant </li>
</ul>
&nbsp;
<h2 data-section-id="f5ej1k" data-start="1475" data-end="1513">How to choose transfection reagents</h2>
&nbsp;
Chemical: lipofection
Cationic lipid (lipofection) reagents form positively charged complexes with nucleic acids that interact with the negatively charged cell membrane, enabling entry primarily via endocytosis. Examples include DOTMA-based formulations and product lines such as Lipofectamine and X-tremeGENE, suitable for a diverse range of cell lines with DNA, small RNA, and CRISPR/Cas9 components. Lipid transfections adapt well to cost-effective and high-throughput formats but are generally cell-type-specific in optimal performance &mdash; a reagent validated on one line may need re-optimization for another.
Chemical: PEI and calcium phosphate
Polyethyleneimine (PEI) is a polymeric transfection agent that, like cationic lipids, forms complexes with DNA for cell membrane interaction &mdash; commonly used for high-yield transient transfection in suspension cultures for recombinant protein production. Calcium phosphate co-precipitation is a long-established method (Graham &amp; van der Eb, 1973) that remains highly efficient for a wide range of cell types and molecules, suitable for both transient and stable transfection, though the process can be harsher on sensitive cell lines, leading to increased cell death in some cases.
Physical: electroporation
Electroporation exposes cells to electrical pulses that create temporary membrane pores, allowing direct entry of DNA, RNA, and other molecules &mdash; an easy, non-chemical technique yielding high efficiency across diverse cell types. Optimization centers on three parameters: pulse voltage, pulse width, and pulse number, with the goal of maintaining 40-80% cell survival. Electroporation requires roughly five-fold more cells and DNA than calcium phosphate methods, typically 1-5 &micro;g DNA per 10⁷ cells, with good linear correlation between DNA amount and uptake.
Viral transduction enhancers
For viral transduction (lentiviral, adenoviral, AAV), polybrene and DEAE-dextran are polycations that minimize electrostatic repulsion between negatively charged viral particles and cell membranes, enhancing transduction efficiency. FBS has been shown to yield better transduction efficiency than bovine calf serum in some transduction protocols &mdash; a media-composition detail relevant to viral workflows specifically.
Selecting reagent for nucleic acid type
Some reagents are specifically optimized for plasmid DNA (Effectene, TransIT-X2), while others (Lipofectamine RNAiMAX) are designed for small oligonucleotides like siRNA. Co-transfection of multiple nucleic acid types may require protocols and ratios validated specifically for that combination rather than direct extrapolation from single-nucleic-acid optimization.
Pre-transfection cell preparation
Regardless of method, cells should be at least 90% viable and have recovered at least 24 hours post-subculture before transfection. Cell density must avoid both contact inhibition (too high, reducing uptake) and insufficient cell-cell contact (too low, reducing growth) &mdash; both extremes reduce efficiency.
&nbsp;
<h2 data-section-id="1fljvas" data-start="5389" data-end="5414">Specifications context</h2>
&nbsp;
Electroporation optimization targets 40-80% post-pulse cell survival across a parameter space of pulse voltage, width, and number, with typical DNA loading of 1-5 &micro;g per 10⁷ cells showing linear uptake correlation within that range. Calcium phosphate transfection (Graham &amp; van der Eb, 1973) remains a foundational method still in active use for both transient and stable transfection across many cell types. As of 2026, lipid-based reagents remain the default first choice for adherent cell line transfection due to ease of use, with electroporation and viral transduction reserved for hard-to-transfect primary/stem cells and applications requiring stable genomic integration respectively.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP and find premium transfection reagents for your lab today.

Transfection Reagents

DNAfectin™ Plus Transfection Reagent

G2500 by Applied Biological Materials (ABM) is DNAfectin™ Plus Transfection Reagent (1.0 mL), a polycation-liposome combination for plasmid DNA transfection in adherent mammalian cells (HeLa, 293T, COS-7, primary cells) — serum-compatible, low cytotoxicity, co-transfection capable, and validated for 96-well and 384-well HTS formats. Store at 4°C; stable 10 days at RT.

Unit Quantity

Application

<h2>What Are Nucleases?</h2>
&nbsp;
Nucleases are enzymes that cleave phosphodiester bonds between nucleotide subunits in nucleic acids. Deoxyribonucleases (DNases) act on DNA; ribonucleases (RNases) act on RNA; and some nucleases (S1 Nuclease, Mung Bean Nuclease) act on both single-stranded DNA and RNA. Nucleases are further divided into endonucleases, which cleave internal phosphodiester bonds, and exonucleases, which progressively remove nucleotides from the 5' or 3' terminus of a strand. Major research nucleases include DNase I (non-specific endonuclease for both ss- and ds-DNA), RNase A (cleaves single-stranded RNA at pyrimidine residues), RNase H (degrades the RNA strand of RNA: DNA hybrids), Exonuclease III (removes nucleotides from 3'-recessed or blunt ends of duplex DNA), S1 Nuclease (degrades single-stranded DNA or RNA), and RNase R (degrades linear RNA but not circular RNA). Match the nuclease to the substrate type (single-stranded vs. double-stranded, DNA vs. RNA), the directionality required (endo vs. exo), and cofactor compatibility with your reaction buffer.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
PureRec RNase A (Recombinant): A ribonuclease free of animal-derived components, designed for the swift elimination of RNA from DNA samples, ensuring no threat of viral contamination.
</li>
<li dir="ltr" aria-level="1">
DNase I Set (RNase-free): A highly effective endonuclease formulated for complete DNA digestion in delicate RNA applications while preserving the integrity of the transcript.
</li>
<li dir="ltr" aria-level="1">
PureRec Duplex-Specific Nuclease (DSN): A unique enzyme designed to equalize high-abundance transcripts, greatly enhancing the identification of rare variants in cDNA libraries.
</li>
<li dir="ltr" aria-level="1">
RNase R: A distinctive 3'-to-5' exoribonuclease that breaks down linear RNA but preserves circular RNA forms, crucial for the identification of circRNA.
</li>
<li dir="ltr" aria-level="1">
RNaseOFF Inhibitor: A strong protein-based barrier that counteracts a wide range of RNases, maintaining the delicate transcriptional environment of your samples.
</li>
</ul>
&nbsp;
<h2>How to Choose Nucleases</h2>
&nbsp;
DNase selection
DNase I (bovine pancreatic, recombinant) non-specifically cleaves both single- and double-stranded DNA in the presence of Mg2+ (random cleavage) or Mn2+ (double-strand cuts at approximately the same position on both strands). Use RNase-free DNase I for RNA preparations to remove genomic DNA contamination. Use Plasmid-Safe ATP-Dependent DNase to selectively degrade linear double-stranded DNA while preserving supercoiled and relaxed circular plasmid DNA. Baseline-ZERO DNase is optimized for the complete removal of trace DNA without damaging RNA.
RNase selection
RNase A cleaves single-stranded RNA at pyrimidine (C and U) residues to produce 3'-pyrimidine nucleotides; it is used to remove RNA from DNA preps, to perform RNase protection assays, and to analyze RNA secondary structure. RNase H degrades the RNA strand of RNA: DNA duplexes and is used after first-strand cDNA synthesis to remove the mRNA template. RNase R (3'-&gt;5' exoribonuclease) degrades all linear RNA without affecting circular RNA, and is used in circular RNA enrichment workflows and to characterize circular RNA splice junctions. Terminator 5'-Phosphate-Dependent Exonuclease (Terminator) digests linear RNA with a 5'-monophosphate, enriching for capped mRNA or circular RNA.
Exonuclease selection
Exonuclease III (Exo III) removes nucleotides from the 3' terminus of duplex DNA and generates single-stranded regions used in unidirectional nested deletions, mapping regulatory elements, and generating single-stranded templates for sequencing. Exonuclease I (Exo I) digests single-stranded DNA in the 3'-&gt;5' direction and is used after PCR to remove unincorporated primers before Sanger sequencing. Lambda Exonuclease removes nucleotides from 5'-phosphorylated duplex DNA in the 5'-&gt;3' direction and is used in isothermal amplification and strand displacement workflows.
Cofactor and inhibitor sensitivity
DNase I requires Mg2+ or Ca2+ for activity and is completely inhibited by EDTA at a 5 mM concentration. RNase A is active across a wide pH range (optimum 7.0-7.5) and is exceptionally stable; inactivation requires proteinase K digestion, phenol-chloroform extraction, or RNase inhibitor protein. RNase H requires Mg2+; RNase R requires Mg2+ and is most active between 37-50 degrees C.
Purity and application grade
For RNA-focused applications, all nucleases used on DNA substrates must be certified RNase-free. For DNA-focused work, DNase-free grades of RNases are required. Confirm lot-specific QC contamination data from the manufacturer before use in sensitive workflows.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
DNase I activity is expressed in Kunitz units: 1 unit increases A260 by 0.001 per minute per mL during DNA hydrolysis at pH 5.0. RNase A activity is expressed as the amount of enzyme hydrolyzing 50% of an RNA substrate under defined conditions; it remains active at a wide range of temperatures and is notoriously difficult to inactivate (requires boiling in SDS, proteinase K digestion, or phenol-chloroform extraction). RNase R from E. coli degrades linear RNA processively in the 3'-&gt;5' direction and is used at 2-10 U per microgram of total RNA to enrich for circular RNA species. RNase R treatment before library construction is the standard method for circular RNA sequencing (circRNA-seq) workflows on both short-read and long-read platforms. For protecting RNA from RNase contamination during nuclease-adjacent workflows, see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/enzymes-and-inhibitors">RNase inhibitors</a>&nbsp;in the parent Enzymes and Inhibitors category.
&nbsp;
Safeguard your molecular images&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> now to obtain a quote for our sophisticated nucleases and inhibitors.

Nucleases

DataSheet

DNase I

Concentration

Note

Caution

MSDS - DNase I (RNase-Free)

DNase I (RNAse-Free)

Protocol

DNase I Set (RNase-free)

E1010 by Zymo Research is a recombinant DNase I Set (RNase-free), supplied lyophilized with DNA Digestion Buffer. One unit = 12 Kunitz units. Guaranteed RNase-free and protease-free; stable through ≥10 freeze-thaw cycles. Heat-inactivated at 75°C/10 min. Available in 250 U, 1500 U, and 5×1500 U.

Unit Definition

Storage

Reconstitution

Inactivation

DNase I Set (250 U) w/ DNA Digestion Buffer (4 ml)

DNase I Set (1500 U) w/ DNA Digestion Buffer (4 ml)

DNase I Set (5 x 1500 U) w/ DNA Digestion Buffer (16 ml)

Tubes

DNase/RNase-Free Tubes (1.5 ml)

C2001-100 by Zymo Research is a 100-tube pack of DNase/RNase-free 1.5 ml polypropylene microcentrifuge tubes suitable for RNA isolation, DNA extraction, and molecular biology sample storage. Manufactured in the USA; also available as C2001-50 (50-tube pack).

DNase/RNase-Free Tubes (1.5 ml) (100 Tubes)

DNase/RNase-Free Tubes (1.5 ml) (50 Tubes)

<h2 dir="ltr"> What Are Miscellaneous PCR Items?</h2>
 Miscellaneous Items groups supporting reagents used alongside core PCR enzymes, master mixes, and nucleotide components, covering essential but non-core materials required for PCR and RT-PCR workflows. This includes nuclease-free water for reaction setup and dilution, Random Primer (6N) and Oligo(dT)18 primers for cDNA synthesis, and ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ used for reaction optimisation. The category also includes certified nuclease-free water options from suppliers such as BIOLOGIX for sensitive molecular biology applications. Use this category to source essential supporting reagents that complete PCR-based experimental workflows alongside primary amplification products. 
&nbsp;
<h2 data-start="432" data-end="459">What you will find:</h2>
&nbsp;
<ul data-start="461" data-end="1220">
<li data-section-id="b5abnd" data-start="461" data-end="635">Nuclease-Free Water: DNase/RNase-free water suitable for PCR, RT-PCR, qPCR, and nucleic acid preparation workflows, including certified molecular biology-grade options.</li>
<li data-section-id="n5mfl0" data-start="636" data-end="789">Primers &amp; Oligos: Random Primer (6N) and Oligo(dT)18 formats for reverse transcription, cDNA synthesis, and related molecular biology applications.</li>
<li data-section-id="1x29qzm" data-start="790" data-end="932">Reaction Components: Molecular biology-grade MgCl₂, MgSO₄, MnCl₂, and NaCl solutions for reaction preparation and protocol optimisation.</li>
<li data-section-id="16hzrat" data-start="933" data-end="1075">Workflow Support Reagents: Essential supporting materials used alongside PCR enzymes, master mixes, and nucleic acid amplification kits.</li>
<li data-section-id="4t6tsy" data-start="1076" data-end="1220">Research-Ready Formats: Convenient reagent sizes suitable for routine academic, biotechnology, and molecular biology laboratory workflows.</li>
</ul>
<h2 dir="ltr"> How to Choose Miscellaneous PCR Items</h2>
 Gel Loading Dyes Loading dyes add density and colour to PCR products for gel electrophoresis, allowing visual tracking of sample migration. Some 2x master mixes include loading dye pre-formulated, but a separate loading dye is needed when using dye-free master mixes or individual-component PCR setups. PCR Enhancers and Additives Additives such as betaine, DMSO, or proprietary enhancer formulations can improve amplification of GC-rich or otherwise difficult templates by reducing secondary structure or improving primer annealing specificity -- useful as a troubleshooting tool when a standard reaction underperforms on a specific target. Nuclease-Free Water Grade Nuclease-free water is used for reaction dilutions, resuspending primers and probes, and as a no-template control in PCR and qPCR setups. Confirm the water is certified free of detectable RNase and DNase activity, particularly for RNA-handling workflows. PCR Tube and Plate Format PCR tubes, strips, and plates are available in formats matching common thermal cycler block configurations -- 0.2 mL individual tubes, 8-well or 12-well strips, and 96-well plates -- select the format matching your thermal cycler and throughput needs. Compatibility with Primary Reagents When ordering supporting consumables alongside primary PCR reagents, confirming tube/plate compatibility with both your thermal cycler and any downstream automation (liquid handlers, plate readers) avoids workflow bottlenecks from mismatched formats. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Nuclease-free water used in molecular biology workflows is typically certified through DNase/RNase activity testing and supplied in formats suitable for routine PCR, RT-PCR, and nucleic acid dilution applications. Random Primer (6N) and Oligo(dT)18 primers are used in reverse transcription workflows to support cDNA synthesis from RNA templates. Ionic solutions such as MgCl₂, MgSO₄, NaCl, and MnCl₂ are used to support and optimise polymerase activity under different reaction conditions. Most items in this category are stable under standard laboratory storage conditions, with product-specific handling and storage requirements provided by the manufacturer.
&nbsp;
Ensure your experimental stability&mdash;<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">reach out to the MBP team</a> now for a quote on our expert PCR accessories

Miscellaneous Items

DNase/RNase-Free Water

W1001 by Zymo Research is DNase/RNase-free water for use in all DNA, RNA, and molecular biology applications. Available in 1, 4, 6, 10, and 30 ml formats (W1001-1 through W1001-30), manufactured in the USA with certificate of analysis. Lab managers and academic researchers running RNA, NGS, or qPCR workflows rely on W1001 as a traceable, nuclease-free aqueous reagent from a trusted supplier.

DNase/RNase-free Water (1 ml)

DNase/RNase-free Water (10 ml)

DNase/RNase-free Water (30 ml)

DNase/RNase-free Water (4 ml)

DNase/RNase-free Water (6 ml)

<h2 data-section-id="16gwf6l" data-start="0" data-end="46">What Are Cold Storage Coolers and Chillers?</h2>
&nbsp;
Laboratory cold storage coolers and chillers are bench-top temperature-control systems designed to keep temperature-sensitive reagents, enzymes, antibodies, cells, and biological samples cold during active laboratory work. They maintain low temperatures&mdash;typically around 0 &deg;C or below freezing&mdash;without requiring ice, electricity (in passive systems), or immediate access to a freezer. These systems are widely used in molecular biology, PCR setup, nucleic acid extraction, enzyme handling, and cell-based assays where maintaining cold-chain conditions during pipetting and multi-step workflows is essential.
Gel-based benchtop coolers are the most common type. They are pre-frozen at approximately &ndash;20 &deg;C and use phase-change materials to maintain samples near 0 &deg;C for several hours, typically up to four hours depending on ambient conditions. This provides a clean, stable, and consistent cooling environment without melting ice or water contamination.
&nbsp;
<h2 dir="ltr">What you will find: </h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Benchtop tube coolers for PCR reagents and enzymes
</li>
<li dir="ltr" aria-level="1">
Cryo tube chillers for 0.2 mL to 2.0 mL tubes
</li>
<li dir="ltr" aria-level="1">
Insulated transport coolers for sample movement
</li>
<li dir="ltr" aria-level="1">
Gel-filled cooling blocks for stable temperature control
</li>
<li dir="ltr" aria-level="1">
Mini coolers for short-term cold storage (2&ndash;8&deg;C range)
</li>
<li dir="ltr" aria-level="1">
Freezer-compatible chillers for sub-zero handling
</li>
<li dir="ltr" aria-level="1">
Rack-style coolers for organized tube placement
</li>
<li dir="ltr" aria-level="1">
Portable cold boxes for field and clinical use
</li>
</ul>
&nbsp;
<h2 data-section-id="1ff2itk" data-start="1171" data-end="1221">How to Choose Cold Storage Coolers and Chillers</h2>
&nbsp;
Tube Format Compatibility
Coolers and chillers are designed around specific laboratory tube formats. Common configurations include holders for 0.2 mL PCR tubes and strips, 1.5/2.0 mL microcentrifuge tubes, 15 mL conical tubes, and 50 mL conical tubes. Some dual-sided designs combine multiple formats in a single unit, allowing simultaneous use for PCR plates on one side and microtubes on the other, improving workflow efficiency and reducing bench clutter.
Temperature Hold and Duration
Passive gel-based coolers maintain approximately 0 &deg;C for several hours after being pre-chilled at &ndash;20 &deg;C. Their performance depends on ambient temperature, workload, and sample frequency. Metal cooling blocks pre-chilled at &ndash;80 &deg;C can achieve lower temperatures temporarily but warm more quickly. For workflows requiring long-duration or continuously controlled cooling, electrically powered thermoelectric chillers provide stable setpoints over extended periods.
Ice-Free vs Ice-Based Systems
Gel-based coolers eliminate the need for ice entirely, reducing condensation, contamination risk, and constant ice replenishment during experiments. Traditional ice buckets remain a low-cost option but require continuous maintenance and can introduce water exposure risks. Ice-free systems are preferred in workflows where moisture control and consistent temperature stability are critical.
Single-Sided vs Dual-Sided Designs
Single-sided coolers are suitable for workflows using one tube format consistently. Dual-sided coolers accommodate multiple formats&mdash;such as PCR plates and microtubes&mdash;within a single unit, making them more versatile for complex molecular biology workflows. Selection depends on workspace constraints and experimental diversity.
Dry Ice Compatibility
Some insulated cold storage systems are designed for use with dry ice, maintaining extremely low temperatures around &ndash;78 &deg;C. These are used for short-term cryogenic handling, transport, or dispensing workflows where ultra-low temperature maintenance is required without active refrigeration.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3892" data-end="3917">Specifications Context</h2>
&nbsp;
Modern gel-based benchtop coolers are engineered using phase-change materials that stabilize near 0 &deg;C for several hours after being pre-conditioned at &ndash;20 &deg;C. Dual-format systems have become increasingly common in molecular biology laboratories because they reduce the number of separate cooling devices required on the bench. As of 2026, these ice-free systems are widely adopted in PCR and nucleic acid workflows due to their consistency, cleanliness, and ease of use compared to traditional ice-based cooling methods.
<a href="https://mbpinc.net/contact-us">Connect with the MBP team</a> today to discuss your specific temperature-hold requirements and find the ideal chiller configuration for your laboratory&rsquo;s daily demands.

Cold Storage Coolers & Chillers

DRYCHILL™ ICE-FREE COOLING BLOCKS

Ice-free, stable cooling for sample tubes\r\nUnique design reduces condensation

Temperature

Capacity

Dimensions (DC9602 + DC4020)

Dimensions (DC0650 + DC1215)

Weight

Warranty

DryChill™ Ice-free Cooling Block, 96 x 0.2ml

DryChill™ Ice-free Cooling Block, 40 x 1.5/2.0ml

DryChill™ Ice-free Cooling Block, 12 x 15ml

DryChill™ Ice-free Cooling Block, 6 x 50ml

<h2>What is Chemicals as a reagent category?</h2>
&nbsp;
Chemicals covers the broad range of chemical and biochemical reagents used across molecular biology, cell biology, biochemistry, and general laboratory work: biochemically relevant compounds like vitamins, buffer salts, amino acids, and enzyme substrates; general laboratory chemicals including solvents, acids, bases, and inorganic salts; and research-grade specialty chemicals for specific applications. Unlike application-specific categories such as Epigenetics or Electrophoresis, which organize reagents by research workflow, Chemicals organizes by the nature of the material itself.
&nbsp;
<h2 dir="ltr">What you'll Discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents" title="Biochemical Reagents:">Biochemical Reagents:</a> High-quality metabolic inducers such as IPTG (G116), L-Arabinose (A2001-ZYM), and Cumate (CH065), along with essential buffers and enzyme stabilizers like TRIS (CH059), DTT (G025), and PMSF (G034).
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/inorganic-reagents-and-compounds" title="Inorganic Reagents &amp; Compounds:">Inorganic Reagents &amp; Compounds:</a> Ultra-pure essential chemical substances like Sodium Azide (CH052) designed for accurate laboratory buffer enhancement and sample conservation methodologies.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/detergents-and-surfactants" title="Detergents &amp; Surfactants:">Detergents &amp; Surfactants:</a> Enhanced cell lysis and denaturing agents featuring 20% SDS (CH100) and concentrated 10X Tris-Glycine-SDS Buffer (CH125) for protein electrophoresis setup.
</li>
</ul>
&nbsp;
<h2>How to navigate Chemicals</h2>
&nbsp;
Start with Biochemical Reagents for biologically relevant compounds
Biochemical Reagents covers the subset of the catalog most relevant to molecular and cell biology workflows: vitamins and cofactors, buffer salts and ready-to-use buffer solutions, amino acids, and other compounds with specific roles in biological systems.
Check purity grade requirements before ordering a research chemical
Research-grade chemicals vary in purity from technical grade to ACS-certified, HPLC-grade, and molecular biology grade, with the appropriate grade depending on how the chemical is used; cell culture and enzymatic applications generally require higher purity than non-biological analytical or general lab use.
Confirm whether your chemical needs are better served by a specialty category
Some research chemicals belong more naturally in application-specific sections of the catalog, such as chromatin immunoprecipitation buffers in Epigenetics or gel running buffers in Electrophoresis, while general-purpose chemicals and biochemical reagents not tied to a single workflow type belong in this Chemicals branch.
Consider bulk ordering for high-consumption research chemicals
Common research chemicals like buffer salts, solvents, and vitamins are used in large quantities by active research labs, and bulk pricing can represent meaningful savings on frequently replenished reagents.
Look for certificates of analysis when accuracy matters for your application
Quantitative applications, including analytical standards, enzyme kinetics studies, and defined cell culture supplementation, require chemicals with documented lot-specific purity and concentration, typically confirmed by certificate of analysis provided with each lot.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Research-grade purity designations for laboratory chemicals follow widely adopted convention systems including ACS grade (meets American Chemical Society purity specifications), HPLC grade (verified purity for chromatographic applications), and molecular biology grade (certified free from nuclease, protease, and other activities that would interfere with molecular biology applications). As of 2026, pre-made, ready-to-use solutions for common biochemical reagents like PBS, HEPES buffer, and Tris-HCl continue to see growing adoption over powder or concentrated formats in labs prioritizing convenience and reproducibility over reagent cost per use.
<a href="https://mbpinc.net/contact-us">Reach out to the friendly team at MBP</a> now to get a quote for your particular research needs.

Chemicals

Guarantee

Shipping condition

Orbital Shaker Accessories

<h2>What are orbital shaker accessories?</h2>
&nbsp;
Orbital shaker accessories are the platform attachments and holders that secure specific vessel types to a shaker platform during operation. Erlenmeyer flasks typically require spring-clip clamps that hold the flask securely while allowing unrestricted liquid movement, microplates use dedicated plate holders, tube rack inserts accommodate centrifuge and microcentrifuge tubes, and non-slip mats provide flexible placement for vessels during lower-speed mixing applications.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>
Dedicated Flask Platforms: Pre-configured platforms designed to securely hold specific sizes of Erlenmeyer flasks or standard microplates.
</li>
<li>
Universal Magnetic Platforms: Versatile, metallic base platforms engineered for instant, tool-free swapping of magnetic clamps and tube racks.
</li>
<li>
Magnetic Flask Clamps: Single-size and combo magnetic clamps designed for quick attachment to hold 25ml up to 500ml Erlenmeyer flasks.
</li>
<li>
Magnetic Tube Racks: Specially designed magnetic racks to accommodate culture tubes, microplates, and large conical bottles in various orientations.
</li>
</ul>
&nbsp;
<h2>How to choose orbital shaker accessories</h2>
&nbsp;
Select Erlenmeyer flask clamps by flask volume
Flask clamps are designed for specific flask sizes, including 125 mL, 250 mL, 500 mL, 1 L, and larger culture flasks. Each clamp is engineered to fit the dimensions of a particular flask size, ensuring secure retention during shaking.
Confirm compatibility with your shaker platform
Accessory mounting systems vary between manufacturers and shaker models. Before ordering, verify that the clamp, holder, or rack is listed as compatible with the specific orbital shaker platform being used.
Choose a microplate holder suitable for your application
Microplate holders secure standard microplates during incubation and mixing procedures. Different holder designs support different operating speeds and plate formats, making compatibility and intended use important selection criteria.
Use a non-slip platform mat for flexible vessel placement
Non-slip mats provide a simple solution for holding bottles, flasks, trays, and other containers at low to moderate shaking speeds. They are useful when vessel configurations change frequently and dedicated clamps are not required.
Verify tube rack capacity and platform fit
Tube rack inserts are available for common laboratory tube formats, including microcentrifuge tubes and conical centrifuge tubes. Confirm that the rack dimensions fit within the shaker platform and that the rack accommodates the desired number of samples per run.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Orbital shaker accessories are designed to keep vessels securely positioned while exposed to continuous circular motion. Flask clamps, plate holders, and tube racks should be selected according to vessel dimensions, operating speed, and platform compatibility. Stainless steel spring-clip clamps remain one of the most widely used accessory formats for laboratory culture applications due to their durability and secure retention.
<a href="https://mbpinc.net/contact-us">Conatct helpful team at MBP</a> for a quick customised quote.

Dedicated Platform, 12 x 125ml Erlenmeyer flasks

The H1000-P-125 by Benchmark Scientific is a dedicated platform for Benchmark Orbi-Shaker and Incu-Shaker orbital shakers, holding 12 x 125 ml Erlenmeyer flasks in a fixed-position configuration for standardized multi-flask culture runs.

Manufacture

Dedicated Platform, 5 x 500ml Erlenmeyer flasks

The H1000-P-500 by Benchmark Scientific is a dedicated orbital shaker platform for 5 × 500 mL Erlenmeyer flasks, compatible with the Incu-Shaker Mini, Orbi-Shaker, and Orbi-Shaker CO2. Flask-specific seating prevents shifting during orbital shaking and supports parallel cell culture, microbial growth, and expression workflows at the 500 mL scale.