Brands

Dry Baths Accessories

<h2>What Are Dry Bath Incubator Accessories?</h2>
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Dry bath accessories are the secondary components that extend or optimize the functionality of a dry block heater: aluminum insert blocks that define the tube format capacity; heated lids that eliminate condensation; external temperature reference probes for calibration verification; and carrying and storage cases for portability. Because the aluminum insert block physically determines which tube formats the dry bath can process, insert block selection is the single most impactful purchase decision after the base unit itself. Most manufacturers supply one insert format as standard with the base unit; labs processing multiple tube formats must purchase additional inserts separately. Select accessories based on the tube formats used in your primary protocols, confirming that inserts are brand- and model-compatible before ordering.
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<h2 dir="ltr">What You Will Find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Specialized Heating Blocks: High-conductivity aluminum blocks tailored for everything from 0.2ml PCR strips and 1.5ml centrifuge tubes to larger 50ml conical vessels.
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Custom &amp; Solid Block Options: Versatile solid blocks suitable for slides or custom machining, alongside blocks specifically drilled for HPLC vials and cuvettes.
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Precision Monitoring &amp; Power: External temperature probes for direct sample tracking and 12V vehicle adapters to maintain thermal control during mobile laboratory work.
</li>
</ul>
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<h2>How to Choose Dry Bath Accessories</h2>
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Aluminum insert blocks: format selection
Insert blocks are precision-machined from aluminum to accept specific tube formats with tight well tolerances (typically within plus or minus 0.1 mm of the tube's outer diameter). Common formats include: 24 wells for 0.5 mL tubes; 15-16 wells for 1.5 mL microcentrifuge tubes; 12-15 wells for 2 mL microcentrifuge tubes; 8-strip configuration for 0.2 mL PCR strip tubes; 12 wells for 15 mL conical tubes; 6 wells for 50 mL conical tubes; and a flat surface (no wells) for microplates or irregular vessels. Inserts are not universally interchangeable across brands; confirm the insert fits the specific dry bath model's block holder dimensions and, for models with auto-recognition, that the insert carries the required auto-recognition contact.
Insert material and thermal conductivity.
Aluminum (Al 6061 alloy) is the standard material for dry bath inserts due to its high thermal conductivity (approximately 167 W/m K), low density, and machinability for tight tolerances. Some specialty inserts for aggressive chemical applications use hard-anodized aluminum or stainless steel, with lower thermal conductivity but better chemical resistance. Always use manufacturer-supplied or manufacturer-approved aluminum inserts; after-market inserts with inferior tolerances reduce thermal contact and degrade temperature uniformity.
Heated lid accessories
Heated lid accessories for dry baths are sold separately for many base unit models or as an upgrade kit. When selecting a heated lid, confirm: (1) it fits the specific block footprint and height of your dry bath model; (2) the lid temperature range covers the required anti-condensation temperature (typically 60-110 degrees C); (3) the lid temperature is independently controlled from the block; and (4) the lid surface material is non-reactive with samples. For protein and enzyme protocols, a lid temperature 5-10 degrees C above the block temperature is the typical starting point.
External temperature reference probe
An external calibration probe (typically a thin RTD or thermocouple probe rated for direct insertion into a tube well, connected to a calibrated digital reference thermometer) verifies that the temperature inside the well matches the instrument's displayed setpoint. External probe verification is required for GLP-compliant and regulated laboratory protocols (ISO 9001, CLIA, cGxP) and is good practice annually for any research dry bath. Confirm that the probe diameter fits the insert well format (most probes are 2-5 mm diameter; well diameter varies by tube format) before ordering.
Carrying case for portable dry baths
Mini and portable dry bath models (Labnet PR1MA Mini, and similar) are available with dedicated carrying cases for field applications: point-of-care diagnostics, on-site environmental sampling, and field isothermal PCR or LAMP assays using a 12 V DC automotive power adapter. Carrying cases provide impact protection, secure block holder positioning, and cable management for field use.
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<h2>Specifications Context</h2>
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Aluminum insert block well tolerances of plus or minus 0.1 mm ensure full circumferential contact between the tube outer wall and the block well, maximizing heat transfer and minimizing temperature gradients between the sample and the block setpoint. A loose-fitting insert with 0.5 mm or more of air gap between tube and well reduces heat transfer and can introduce a temperature lag of 1-3 degrees C compared to the block setpoint, systematically underheating samples. Heated lid accessories eliminate condensation-related volume errors of 5-15% that can occur in short (&lt;10 microliter) enzyme reactions at 37-55 degrees C over 30-60 minute incubations. Auto-recognized interchangeable block inserts (where the dry bath unit automatically detects which format insert is installed) are a standard feature on Labnet and similar mid-range dry bath models, improving setup speed and reducing configuration errors.&nbsp;
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Enhance your dry bath setup today&mdash;check out our full range of blocks and <a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> for a quote.

Custom drilling for BSW01 (sold separately), up to 36 holes

BSW01-DR by Benchmark Scientific is a custom-drilling service for the BSW01 solid aluminum dry-bath block (sold separately as SKU BSW01), drilling up to 36 holes to a customer-specified pattern and tube diameter. The resulting block fits Benchmark BSH1001, BSH1002, and BSH1004 digital dry baths and accommodates non-standard tube formats, specialty vials, or custom labware not available in the standard block range. Contact MBP with your hole specifications before ordering: <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a> or +1 416-549-8100.

Manufacture

Cuvettes

<h2 dir="ltr">What are Cuvettes?</h2>
 Cuvettes are small, precision-dimensioned transparent vessels used to hold liquid samples in the light path of a spectrophotometer, fluorometer, or colorimeter for measurement of absorbance, transmittance, fluorescence intensity, or fluorescence polarization. The standard cuvette has a 10 mm optical path length (the distance light travels through the sample), a square or rectangular cross-section, and an internal volume of 1&ndash;4 mL. Cuvettes are manufactured in four main materials&mdash;polystyrene (PS) or polymethylmethacrylate (PMMA) plastic, optical glass, UV-grade quartz (fused silica), and IR quartz&mdash;each covering a different wavelength range. Choose plastic cuvettes for routine visible-light absorbance (380&ndash;780 nm) where disposability prevents cross-contamination; choose optical glass for reusable visible-range work (340&ndash;2,500 nm); choose UV quartz when measurements extend into the UV range below 340 nm (190&ndash;2,500 nm).
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="1cg0wqn" data-start="1810" data-end="1862">Spectrophotometer cuvettes for absorbance analysis</li>
<li data-section-id="vbobpt" data-start="1863" data-end="1890">Polystyrene (PS) cuvettes</li>
<li data-section-id="14z5a6i" data-start="1891" data-end="1906">PMMA cuvettes</li>
<li data-section-id="1h4vr7o" data-start="1907" data-end="1966">Analyzer-specific cuvettes for clinical chemistry systems</li>
<li data-section-id="b3k6fl" data-start="1967" data-end="1998">Standard path length cuvettes</li>
<li data-section-id="td8a19" data-start="1999" data-end="2043">Accessories for spectrophotometer cuvettes</li>
</ul>
<h2 dir="ltr"> How to Choose Cuvettes</h2>
 Material and Wavelength Range Plastic (PS/PMMA) cuvettes transmit visible light from 380&ndash;780 nm and are single-use, eliminating cleaning and cross-contamination risk. They are unsuitable for UV measurements, organic solvents, or strong acids. Optical glass cuvettes transmit 340&ndash;2,500 nm, covering the full visible and near-infrared range; Different manufacturers recommend different procedures. UV quartz cuvettes are traditionally used for DNA/RNA quantification at OD260/OD280 because they transmit UV wavelengths below 340 nm. IR quartz extends to 3,500 nm for near-IR applications. Optical Path Length The standard 10 mm path length follows the Beer-Lambert law at normal sample concentrations. Semi-micro (5 mm path) and micro-volume cuvettes (1 mm path, or internal volumes down to 10&ndash;70 &micro;L) are used for precious or limited-volume samples. The path length directly multiplies absorbance: a 1 mm cuvette gives 1/10 the absorbance of a 10 mm cuvette for the same solution &mdash; account for this in Beer-Lambert calculations. Volume: Macro vs. Semi-Micro vs. Micro Macro cuvettes (1&ndash;4 mL) are the standard for routine spectrophotometry. Semi-micro cuvettes (0.5&ndash;1.5 mL) reduce sample consumption. Micro cuvettes (10&ndash;350 &micro;L) are used when sample volume is limited; sub-micro cuvettes (10 &micro;L or less) suit precious clinical or research samples. Confirm that your spectrophotometer's beam height and aperture are compatible with the cuvette format before purchasing micro formats. Two-Window vs. Four-Window Standard cuvettes have two optically clear windows (the light-entry and exit faces) and two frosted or ribbed side walls for gripping. Four-window (all-clear) cuvettes are required for fluorescence measurements at 90&deg; to the excitation beam &mdash; the two additional clear windows allow emitted fluorescence to exit perpendicular to the excitation path. Confirm whether your instrument requires a standard or fluorescence-grade cuvette. Reusable vs. Disposable Quartz and glass cuvettes are reusable; their higher up-front cost&nbsp;is justified for low-throughput precision work or when solvents or UV wavelengths are required. Plastic cuvettes cost fractions of a cent per use and eliminate washing time in high-throughput assay workflows. Select disposable plastic for any routine colorimetric assay (Bradford, BCA, ELISA plate reader backup, OD600 bacterial growth) where wavelengths stay in the visible range. 
<h2 dir="ltr"> Specifications Context</h2>
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The 10 mm path length standard cuvette has internal dimensions of 10 &times; 10 mm cross-section and an internal height of approximately 45 mm. Outer dimensions are 12.5 &times; 12.5 mm, fitting all standard spectrophotometer cell holders. Window parallelism tolerance is &plusmn;0.01 mm for precision quartz cuvettes; standard optical glass cuvettes have &plusmn;0.1 mm tolerance. Light transmission through UV quartz: &gt;90% at 220 nm and &gt;85% at 200 nm. For nucleic acid quantification, a UV quartz cuvette with a minimum path length of 10 mm is required for OD 260/280 measurements; the ratio of OD260/OD280 should be 1.8&ndash;2.0 for pure DNA and 1.9&ndash;2.1 for pure RNA as a purity indicator. As of 2026, plastic disposable cuvettes remain the most cost-efficient choice for labs running &gt;50 absorbance readings per day in the visible range.
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Need the right fit? MBP can guide you to cuvettes that match your workflow perfectly. For institutional pricing and PO-based bulk ordering, <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">contact MBP</a>&nbsp;directly.

6618-Analyzer Consumables Catalog

Cuvette for MLA Electra 620, 650 & 750, PS, BOX/550

Globe Scientific 6618 polystyrene cuvettes for MLA Electra 620, 650, and 750 coagulation analyzers, packaged 550 per box. Direct-fit replacement for Electra analyzer cuvettes. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a> or email customerservice@mbpinc.net.

5107-Group-Analyzer Consumables Catalog

Cuvettes for Ciba Corning 550 Express

Globe Scientific 5107 — polystyrene disposable cuvettes for the Bayer 550 Express and 550 Express Plus (Ciba Corning 550 Express) analyzers, packaged in cartridges with 13 five-place segments for fast loading.

CIBA CORNING: Disposal Bag Units, for use with Ciba Corning 550 Express and Express Plus analyzers, CS/24

CIBA CORNING: Cuvette Tray & 24 Disposal Bags, for use with Ciba Corning 550 Express, Express Plus analyzers and Awareness Technology Chemwell T Analyzer, CS/100

CIBA CORNING: Cuvette Tray Only, for use with Ciba Corning 550 Express and Express Plus analyzers (No Disposal Bags), CS/200

5145-Group-Analyzer Consumables Catalog

Cuvettes for Mindray® BS200 & BS300

5145 by Globe Scientific is a polystyrene cuvette segment for Mindray® BS200 and BS300 chemistry analyzers, individually wrapped. Supplied 1/package, 250/box, 1,000/case. A third-party alternative to OEM Mindray cuvettes for high-volume clinical chemistry labs.

MINDRAY: Cuvette Segment for Mindray BS200 analyzer, 1/pk, 250/Box, 1000/Unit, CS/1000

MINDRAY: Cuvette Segment for Mindray BS300 analyzer, 1/pk, 250/Box, 1000/Unit, CS/1000

Storage Boxes & Racks

<h2 data-section-id="1hv97bw" data-start="0" data-end="47">What Are Laboratory Storage Boxes and Racks?</h2>
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Laboratory storage boxes and racks are essential organizational systems used to store, organize, and protect cryogenic vials, microcentrifuge tubes, centrifuge tubes, and microscope slides across freezers, cold rooms, and benchtop environments. Storage boxes are typically lidded, grid-based containers used for long-term sample organization in ultra-low temperature conditions, while racks are open-frame supports used for active handling during laboratory workflows such as pipetting, vortexing, and reaction setup.
Cryogenic storage boxes are commonly designed to hold 81 vials (9&times;9 format) or 100 vials (10&times;10 format), and are compatible with storage conditions ranging from &ndash;196 &deg;C in liquid nitrogen vapor phase to +121 &deg;C for autoclave sterilization (depending on material). Tube racks, on the other hand, provide stable upright positioning of tubes during active benchwork and are not primarily intended for long-term freezer storage.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Cryogenic storage boxes for vials and microtubes
</li>
<li dir="ltr" aria-level="1">
Freezer storage boxes for -20&deg;C and -80&deg;C applications
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<li dir="ltr" aria-level="1">
PCR tube racks for 0.2 mL tubes and strips
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<li dir="ltr" aria-level="1">
Microcentrifuge tube racks for 0.5 mL to 2 mL tubes
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<li dir="ltr" aria-level="1">
Polypropylene (PP) autoclavable storage boxes
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Stackable storage boxes for space-saving organization
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Hinged lid storage boxes for secure sample protection
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Color-coded racks for easy sample identification
</li>
</ul>
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<h2 data-section-id="flyrii" data-start="1159" data-end="1199">How to Choose Storage Boxes and Racks</h2>
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Vial Format and Capacity
The first selection criterion is compatibility with tube size and grid layout. The most common format is the 81-place (9&times;9) box designed for 1.0&ndash;2.0 mL cryovials. Smaller or specialized formats may accommodate 5 mL tubes or different vial diameters depending on grid spacing. Proper matching ensures secure fit, easy retrieval, and consistent sample organization in high-density storage systems.
Temperature Range
Material selection determines allowable storage conditions. Polycarbonate cryogenic boxes are the most versatile, supporting temperatures from liquid nitrogen levels (&ndash;196 &deg;C) through &ndash;80 &deg;C freezer storage and up to autoclave sterilization at +121 &deg;C. Cardboard freezer boxes with moisture-resistant coating are designed for economical freezer storage and can withstand ultra-low temperatures, but they are not suitable for autoclaving or long-term moisture exposure.
Tube Rack Materials and Use
Polypropylene racks are widely used because they are autoclavable and chemically resistant, making them suitable for sterile workflows. ABS and polystyrene racks provide lower-cost alternatives for general laboratory bench use but are not designed for autoclave conditions. For freezer organization, stainless steel racks are often used to hold multiple cryoboxes within &ndash;80 &deg;C upright freezer systems, improving accessibility and spatial organization.
Slide Storage Boxes
Microscope slide storage boxes are designed to protect standard 25 &times; 75 mm slides. They are available in 25-, 50-, and 100-slide capacities. Polypropylene versions are autoclavable and suitable for sterilized workflows, while fiberboard or ABS versions are typically used for room-temperature archival storage and transport.
Color Coding and Sample Identification
Color-coded storage boxes are widely used in biobanking and high-throughput laboratories to quickly differentiate sample groups without opening boxes or reading labels at freezer temperatures. Common systems include multiple lid colors paired with alphanumeric grids inside the box, enabling precise sample tracking and retrieval in large inventories.
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<h2 data-section-id="1jl751w" data-start="4067" data-end="4092">Specifications Context</h2>
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Modern polycarbonate cryogenic storage boxes are engineered for extreme temperature stability, typically rated from &ndash;196 &deg;C to +121 &deg;C, allowing use across liquid nitrogen vapor phase storage, &ndash;80 &deg;C freezers, and autoclave sterilization cycles. Cardboard freezer boxes are coated to resist moisture ingress and are optimized for cost-efficient high-density storage in ultra-low temperature environments.
The 81-place (9&times;9 grid) polycarbonate cryobox remains the standard format in most biorepositories and clinical research facilities due to its compatibility with SBS footprint freezer systems and automated inventory tracking workflows. These systems are widely used in genomics, cell banking, and pharmaceutical research environments.
For product details, specifications, or bulk ordering support, <a href="https://mbpinc.net/contact-us">the MBP team can help you</a> choose the right storage solution for your laboratory needs.

Brochure

Cylinder with Cap for Pipettes, 82 x 426mm

600592 by Globe Scientific — PP cylinder with tight-fitting cap for upright pipette storage. 82 mm diameter × 426 mm height, welded flanged base. Protects serological pipette tips from dust and chipping. Sold individually.

<h2>What are 5-hmC ELISA kits?</h2>
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5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
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<h2>How to use 5-hmC ELISA kits</h2>
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Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
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<h2>Specifications context</h2>
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Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

MSDS

Cytosine DNA Standard (2 µg)

D5405-1 by Zymo Research is the Cytosine DNA Standard (2 µg) containing 100% unmodified cytosines (no 5-mC, no 5-hmC), used to anchor the zero-modification point on calibration curves in 5-mC and 5-hmC ELISA quantitation assays. Compatible with Zymo Research 5-mC DNA ELISA Kit (D5325) and the 5-Methylcytosine &amp; 5-Hydroxymethylcytosine DNA Standard Set (D5405). Store at −20 °C. Made in USA.

Manufacturer

Shipping Condition

Storage Condition

<h2 dir="ltr">What Are Organelle Labeling Lentiviral Particles?</h2>
 Organelle labeling lentiviral particles are ready-to-transduce viral stocks that deliver a fluorescent protein (GFP or RFP) fused to a validated subcellular targeting sequence. After stable integration, transduced cells express a fluorescent marker localised to a specific organelle &mdash; providing a heritable, non-toxic alternative to chemical dyes for subcellular imaging. Cellular imaging studies depend on accurate organelle targeting. Choose a labeling system that supports clear visualization and analysis.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mitochondria labeling viruses for monitoring cellular metabolism and energy dynamics in live-cell studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Nucleus labeling viruses for gene expression analysis and nuclear localization tracking in mammalian cells</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endoplasmic reticulum labeling viruses for studying folding, synthesis, and intracellular transport pathways of proteins</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Golgi apparatus labeling viruses for protein processing and for vesicle trafficking visualization</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Plasma membrane labeling of viruses for cell interaction studies, receptor localization, and membrane dynamics</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Lysosome labeling viruses for autophagy and degradation pathway-related applications</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Peroxisome labeling viruses for oxidative stress response and metabolic studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Cytoskeleton labeling viruses for cell morphology analysis and structural organization studies</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Autophagosome labeling of viruses for cellular recycling pathway and autophagy flux studies</li>
</ul>
<h2 dir="ltr"> How to Choose an Organelle Labeling Lentivirus</h2>
 Target Organelle Available targeting constructs cover mitochondria, endoplasmic reticulum, nucleus, Golgi apparatus, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; select based on the structure of interest in your experiment. Fluorophore Choice GFP- and RFP-based constructs allow flexibility for multiplexed imaging &mdash; pairing an organelle marker with a second fluorescent reporter (e.g. a protein-of-interest fusion) in the red or green channel respectively. Stable vs. Transient Labeling Needs Lentiviral delivery produces stable, integrated reporter expression maintained across cell divisions &mdash; essential for long-term time-lapse imaging, drug treatment time courses, or generation of reporter cell line banks. Cytotoxicity Considerations Unlike chemical dyes (MitoTracker, LysoTracker, ER-Tracker), which can be cytotoxic at working concentrations and require repeated application, lentiviral organelle markers avoid repeated dye loading and chronic toxicity in long-term experiments. Multiplexing Strategy When combining an organelle marker with other fluorescent reporters, select spectrally distinct fluorophores to avoid channel overlap in fluorescence microscopy or flow cytometry. 
<h2 dir="ltr"> Specifications Context</h2>
 Organelle labeling lentiviral particles sourced from Applied Biological Materials (ABM) are supplied as ready-to-transduce stocks, eliminating the need for in-house viral production. Stable genomic integration enables longitudinal analysis of organelle morphology and dynamics across cell passages, treatments, and differentiation time courses. All lentiviral workflows require institutional biosafety committee (IBC) approval, with BSL-2 containment standard for third-generation systems. Product availability reflects MBP&rsquo;s catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>of MBP to confirm availability for specific organelle targets and fluorophore combinations.

Organelle Labeling Viruses

Important Considerations for Lentiviruses

Cytoskeleton Labeling Lentivirus (ACTB)(GFP)

Unit Quantity

Vector

Titer	

Cytoskeleton Labeling Lentivirus (ACTB)(RFP)

Cytoskeleton Labeling Lentivirus (TUBA1B)(GFP)

Cytoskeleton Labeling Lentivirus (TUBA1B)(RFP)

<h2 data-section-id="185nm5c" data-start="0" data-end="38">What is classic cell culture media?</h2>
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Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture for decades, including DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's F-10, Ham's F-12, IMDM (Iscove's Modified Dulbecco's Medium), and DMEM/F-12. These media provide the amino acids, vitamins, glucose, salts, buffering agents, and other nutrients required to support cell survival and growth in vitro. They form the basis of many modern specialized, serum-free, and advanced media formulations used in academic research, biotechnology, pharmaceutical development, and biomanufacturing.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
&nbsp;Availability of both low and high carbohydrate levels of balanced basal formulations for the distinct metabolic requirements of diverse mammalian cell lines.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Maintenance of precise physiological pH during long-term incubation cycles using optimized buffering configurations with integrated chemical stabilizers&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Nutrient-dense cell media supplemented with vitamins and essential amino acids to support&nbsp; hybridomas and rapidly proliferating continuous cultures
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Optimized serum reduction along with enhanced cell attachment using&nbsp;Multi-purpose 1:1 formulation hybrids&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Salt solutions supplemented with essential minerals to facilitate enzyme preparation, cell washing, and reliable short-term osmotic maintenance.
</li>
</ul>
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<h2 data-section-id="1blio5n" data-start="931" data-end="964">How to choose a classic medium</h2>
&nbsp;
DMEM vs MEM
DMEM contains up to four times the amino acids and vitamins found in MEM, along with higher glucose concentrations, making it suitable for cell types with elevated nutrient demands. It is widely used for primary cells, stem cells, and many transformed mammalian cell lines. MEM, based on the original Eagle's formulation, remains a common choice for standard monolayer culture and is available with different salt formulations to support various applications.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640 was originally developed for culturing human leukemic cells and remains the preferred medium for lymphocytes, hybridomas, and other hematopoietic cell types. Its formulation includes glutathione and elevated levels of biotin, vitamin B12, and PABA. Although designed for suspension cultures, RPMI 1640 also supports a wide range of adherent cell lines when appropriately supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were developed for CHO cells and other specialized cell types. Their formulations include additional nutrients such as zinc, putrescine, hypoxanthine, and thymidine, making them useful for hepatocyte culture, toxicity testing, viral fusion studies, and other specialized applications. These media can be used with or without serum depending on the experimental requirements.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 combines the nutrient-rich composition of DMEM with the specialized components found in Ham's F-12. The resulting formulation supports demanding cell types including MDCK cells, glial cells, fibroblasts, and endothelial cells. It is also widely used as the foundation for many serum-free and advanced culture systems.
IMDM for high-density rapid proliferation
IMDM is an enriched derivative of DMEM that contains additional amino acids, vitamins, HEPES buffer, sodium pyruvate, and potassium nitrate. Its enhanced nutrient profile makes it particularly suitable for rapidly proliferating and high-density cultures such as Jurkat cells, COS-7 cells, and macrophages.
Liquid vs powder format
Liquid classic media are supplied ready for use after the addition of serum and required supplements. Powdered media offer lower shipping costs and greater flexibility for high-volume laboratories but require reconstitution, sodium bicarbonate addition, and sterile filtration before use.
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<h2 data-section-id="1fljvas" data-start="4382" data-end="4407">Specifications context</h2>
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Classic media are manufactured according to their original published formulations, with only stability-related modifications where necessary. Most liquid formulations contain sodium bicarbonate as the primary buffering system and require incubation in a 5&ndash;10% CO₂ atmosphere to maintain physiological pH. Powdered formulations require reconstitution, sodium bicarbonate supplementation, and sterile filtration before use.
As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the most widely used classic cell culture media across academic, biotechnology, pharmaceutical, and biomanufacturing laboratories. IMDM continues to gain popularity for high-density culture and bioprocessing applications.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents such as GlutaMAX, NEAA, sodium pyruvate, and HEPES, see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized classic media for your lab.

Classic Media

DMEM/F-12 (1:1) Medium

Sterility

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Caution

<h2>What are DNA Cleanup Kits?</h2>
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DNA cleanup kits purify and concentrate pre-extracted or pre-amplified DNA by removing enzymes, salts, organic solvents (phenol, chloroform), unincorporated nucleotides, RNA, detergents, and other contaminants from DNA solutions using silica-membrane spin columns with a bind-wash-elute protocol. Unlike PCR cleanup kits that are specifically optimized to remove primers and dNTPs from PCR reactions, DNA cleanup kits accept broader input chemistries - including post-phenol-chloroform extraction DNA, genomic DNA in high-salt lysis buffers, restriction digest reactions, and dilute DNA solutions requiring concentration - and elute DNA in minimal volumes (as little as 6 ul) for immediate use in downstream applications. Use a DNA cleanup kit whenever your DNA preparation contains inhibitors incompatible with downstream enzymatic reactions, when residual phenol must be removed, or when dilute DNA must be concentrated into a small volume.
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<h2 dir="ltr">What you will find:</h2>
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DNA Clean &amp; Concentrator&trade; (DCC): Efficient spin-column systems (5, 25, 100, 500 preps) created to concentrate low-concentration DNA into ultra-pure, transfection-quality volumes.
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Genomic DNA Clean &amp; Concentrator: Tailored procedures for extracting large templates while maintaining genomic integrity for long-read sequencing uses.
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Select-a-Size DNA Clean &amp; Concentrator&trade;: Tailored systems for the recovery of size-specific fragments, efficiently eliminating adapter dimers and non-target amplicons.
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ZR-96 DNA Cleanup Systems: Scalable 96-well and MagBead configurations designed for high-throughput, automated processes while ensuring reproducibility-validated outcomes.
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<h2>How to Choose a DNA Cleanup Kit</h2>
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Capacity and Input Volume
Zymo DNA Clean &amp; Concentrator kits are available in formats matched to DNA input mass: DCC-5 (&lt;=5 ug input, elute &gt;=6 ul), DCC-25 (&lt;=25 ug input, elute &gt;=25 ul), DCC-100 (&lt;=100 ug input, elute &gt;=25 ul). Choose the format whose maximum capacity exceeds your expected DNA input by at least 2-fold to avoid column overloading, which reduces yield and purity. For genomic DNA preparations from tissue (typically 5-30 ug), DCC-25 or DCC-100 is appropriate.
Minimum Elution Volume and Concentration
For maximum DNA concentration, Zymo DCC-5 elutes in 6 ul - the industry minimum for standard silica columns. If the input volume is 500 ul (1 ng/ul) and the elution is 6 ul, the final concentration reaches approximately 80 ng/ul (assuming 95% recovery), a concentration suitable for transfection, electroporation, and microinjection.
Size Selectivity
Standard DNA cleanup kits retain all DNA &gt;=100 bp. If size selection is also required, use gel extraction (for specific band isolation) or AMPure XP beads (for size-range selection by bead: volume ratio adjustment) rather than a standard cleanup kit.
Post-Phenol-Chloroform Cleanup
For DNA recovered from phenol-chloroform extraction, add 5 volumes of binding buffer and 2 volumes of 100% ethanol to the aqueous phase before column loading. The organic solvent in residual phenol or chloroform does not significantly impair column binding at these dilutions. Multiple column reloads allow processing of large aqueous-phase volumes through a single column.
RNase A Treatment for DNA-Only Preparations
For gDNA preparations that must be RNA-free for optical mapping or certain long-read sequencing library kits, add RNase A (100 ug/ml) to the DNA solution, incubate at 37 degrees C for 10 minutes, then proceed directly to DCC cleanup. The RNase digestion products are removed during the wash steps.
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<h2>Specifications Context</h2>
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Zymo DNA Clean &amp; Concentrator kits use a single-buffer system with Zymo-Spin column technology and recover DNA from PCR, enzymatic reactions, phenol-chloroform extractions, and dilute solutions. DNA is eluted in volumes as small as 6 ul, providing up to 80-fold concentration from 500 ul inputs. The kits remove enzymes, proteins, salts, organic solvents, dyes, unincorporated nucleotides, primers, and other small molecules, delivering DNA ready for restriction digestion, ligation, sequencing, Southern blotting, transfection, and long-read library preparation. DNA cleanup and concentration are standard workflow steps in genomics laboratories at Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center, where removing trace inhibitors from gDNA before library preparation significantly improves NGS library complexity and uniformity.
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Enhance your capabilities&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP Inc. team</a> now to obtain a quote for your DNA concentration requirements.

DNA Cleanup Kits

Protocol

DNA Clean & Concentrator Magbead Kit (100 preps)

D4012 by Zymo Research is a 100-prep magnetic bead DNA cleanup kit compatible with any open liquid handler or magnetic bead mover. Recovers DNA ≥25 bp (up to 150 kb HMW); &gt;90% recovery for ≥100 bp fragments; purity A260/A280 &amp; A260/A230 ≥1.8; elution in 30–50 µl. Suitable for PCR cleanup, NGS library prep, restriction digests, and automated high-throughput purification.