Brands

<h2>What are 5-hmC ELISA kits?</h2>
&nbsp;
5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
&nbsp;
<h2>How to use 5-hmC ELISA kits</h2>
&nbsp;
Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

MSDS

Control E (100 ng/ul) (40 µl)

D5425-5-5 by Zymo Research is Control E, a mouse tissue-derived genomic DNA at 100&nbsp;ng/&micro;l (40&nbsp;&micro;l) with defined %5-hmC for standard curve construction in the Quest 5-hmC DNA ELISA Kit (D5425). Used alongside Control C (D5425-5-3). Research use only.

Manufacturer

Shipping Condition

Storage Condition

Plates

Conversion Plate w/ Cover Foil (2 Plates/Foils)

C2005 by Zymo Research is a Conversion Plate with Cover Foil for 96-well bisulfite conversion workflows. Each unit includes 2 non-skirted PCR plates (up to 150 µl per well) with 2 piercing-compatible cover foils. Compatible with most standard thermal cyclers.

<h2 data-section-id="16gwf6l" data-start="0" data-end="46">What Are Cold Storage Coolers and Chillers?</h2>
&nbsp;
Laboratory cold storage coolers and chillers are bench-top temperature-control systems designed to keep temperature-sensitive reagents, enzymes, antibodies, cells, and biological samples cold during active laboratory work. They maintain low temperatures&mdash;typically around 0 &deg;C or below freezing&mdash;without requiring ice, electricity (in passive systems), or immediate access to a freezer. These systems are widely used in molecular biology, PCR setup, nucleic acid extraction, enzyme handling, and cell-based assays where maintaining cold-chain conditions during pipetting and multi-step workflows is essential.
Gel-based benchtop coolers are the most common type. They are pre-frozen at approximately &ndash;20 &deg;C and use phase-change materials to maintain samples near 0 &deg;C for several hours, typically up to four hours depending on ambient conditions. This provides a clean, stable, and consistent cooling environment without melting ice or water contamination.
&nbsp;
<h2 dir="ltr">What you will find: </h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Benchtop tube coolers for PCR reagents and enzymes
</li>
<li dir="ltr" aria-level="1">
Cryo tube chillers for 0.2 mL to 2.0 mL tubes
</li>
<li dir="ltr" aria-level="1">
Insulated transport coolers for sample movement
</li>
<li dir="ltr" aria-level="1">
Gel-filled cooling blocks for stable temperature control
</li>
<li dir="ltr" aria-level="1">
Mini coolers for short-term cold storage (2&ndash;8&deg;C range)
</li>
<li dir="ltr" aria-level="1">
Freezer-compatible chillers for sub-zero handling
</li>
<li dir="ltr" aria-level="1">
Rack-style coolers for organized tube placement
</li>
<li dir="ltr" aria-level="1">
Portable cold boxes for field and clinical use
</li>
</ul>
&nbsp;
<h2 data-section-id="1ff2itk" data-start="1171" data-end="1221">How to Choose Cold Storage Coolers and Chillers</h2>
&nbsp;
Tube Format Compatibility
Coolers and chillers are designed around specific laboratory tube formats. Common configurations include holders for 0.2 mL PCR tubes and strips, 1.5/2.0 mL microcentrifuge tubes, 15 mL conical tubes, and 50 mL conical tubes. Some dual-sided designs combine multiple formats in a single unit, allowing simultaneous use for PCR plates on one side and microtubes on the other, improving workflow efficiency and reducing bench clutter.
Temperature Hold and Duration
Passive gel-based coolers maintain approximately 0 &deg;C for several hours after being pre-chilled at &ndash;20 &deg;C. Their performance depends on ambient temperature, workload, and sample frequency. Metal cooling blocks pre-chilled at &ndash;80 &deg;C can achieve lower temperatures temporarily but warm more quickly. For workflows requiring long-duration or continuously controlled cooling, electrically powered thermoelectric chillers provide stable setpoints over extended periods.
Ice-Free vs Ice-Based Systems
Gel-based coolers eliminate the need for ice entirely, reducing condensation, contamination risk, and constant ice replenishment during experiments. Traditional ice buckets remain a low-cost option but require continuous maintenance and can introduce water exposure risks. Ice-free systems are preferred in workflows where moisture control and consistent temperature stability are critical.
Single-Sided vs Dual-Sided Designs
Single-sided coolers are suitable for workflows using one tube format consistently. Dual-sided coolers accommodate multiple formats&mdash;such as PCR plates and microtubes&mdash;within a single unit, making them more versatile for complex molecular biology workflows. Selection depends on workspace constraints and experimental diversity.
Dry Ice Compatibility
Some insulated cold storage systems are designed for use with dry ice, maintaining extremely low temperatures around &ndash;78 &deg;C. These are used for short-term cryogenic handling, transport, or dispensing workflows where ultra-low temperature maintenance is required without active refrigeration.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3892" data-end="3917">Specifications Context</h2>
&nbsp;
Modern gel-based benchtop coolers are engineered using phase-change materials that stabilize near 0 &deg;C for several hours after being pre-conditioned at &ndash;20 &deg;C. Dual-format systems have become increasingly common in molecular biology laboratories because they reduce the number of separate cooling devices required on the bench. As of 2026, these ice-free systems are widely adopted in PCR and nucleic acid workflows due to their consistency, cleanliness, and ease of use compared to traditional ice-based cooling methods.
<a href="https://mbpinc.net/contact-us">Connect with the MBP team</a> today to discuss your specific temperature-hold requirements and find the ideal chiller configuration for your laboratory&rsquo;s daily demands.

Cold Storage Coolers & Chillers

CoolCube™ Microtube and PCR Plate Cooler

Temperature

Capacity (side 1)

Capacity (side 2)

Dimensions (WxDxH)

Weight

Warranty

<h2>What is Chemicals as a reagent category?</h2>
&nbsp;
Chemicals covers the broad range of chemical and biochemical reagents used across molecular biology, cell biology, biochemistry, and general laboratory work: biochemically relevant compounds like vitamins, buffer salts, amino acids, and enzyme substrates; general laboratory chemicals including solvents, acids, bases, and inorganic salts; and research-grade specialty chemicals for specific applications. Unlike application-specific categories such as Epigenetics or Electrophoresis, which organize reagents by research workflow, Chemicals organizes by the nature of the material itself.
&nbsp;
<h2 dir="ltr">What you'll Discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/biochemical-reagents" title="Biochemical Reagents:">Biochemical Reagents:</a> High-quality metabolic inducers such as IPTG (G116), L-Arabinose (A2001-ZYM), and Cumate (CH065), along with essential buffers and enzyme stabilizers like TRIS (CH059), DTT (G025), and PMSF (G034).
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/inorganic-reagents-and-compounds" title="Inorganic Reagents &amp; Compounds:">Inorganic Reagents &amp; Compounds:</a> Ultra-pure essential chemical substances like Sodium Azide (CH052) designed for accurate laboratory buffer enhancement and sample conservation methodologies.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/chemicals/detergents-and-surfactants" title="Detergents &amp; Surfactants:">Detergents &amp; Surfactants:</a> Enhanced cell lysis and denaturing agents featuring 20% SDS (CH100) and concentrated 10X Tris-Glycine-SDS Buffer (CH125) for protein electrophoresis setup.
</li>
</ul>
&nbsp;
<h2>How to navigate Chemicals</h2>
&nbsp;
Start with Biochemical Reagents for biologically relevant compounds
Biochemical Reagents covers the subset of the catalog most relevant to molecular and cell biology workflows: vitamins and cofactors, buffer salts and ready-to-use buffer solutions, amino acids, and other compounds with specific roles in biological systems.
Check purity grade requirements before ordering a research chemical
Research-grade chemicals vary in purity from technical grade to ACS-certified, HPLC-grade, and molecular biology grade, with the appropriate grade depending on how the chemical is used; cell culture and enzymatic applications generally require higher purity than non-biological analytical or general lab use.
Confirm whether your chemical needs are better served by a specialty category
Some research chemicals belong more naturally in application-specific sections of the catalog, such as chromatin immunoprecipitation buffers in Epigenetics or gel running buffers in Electrophoresis, while general-purpose chemicals and biochemical reagents not tied to a single workflow type belong in this Chemicals branch.
Consider bulk ordering for high-consumption research chemicals
Common research chemicals like buffer salts, solvents, and vitamins are used in large quantities by active research labs, and bulk pricing can represent meaningful savings on frequently replenished reagents.
Look for certificates of analysis when accuracy matters for your application
Quantitative applications, including analytical standards, enzyme kinetics studies, and defined cell culture supplementation, require chemicals with documented lot-specific purity and concentration, typically confirmed by certificate of analysis provided with each lot.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Research-grade purity designations for laboratory chemicals follow widely adopted convention systems including ACS grade (meets American Chemical Society purity specifications), HPLC grade (verified purity for chromatographic applications), and molecular biology grade (certified free from nuclease, protease, and other activities that would interfere with molecular biology applications). As of 2026, pre-made, ready-to-use solutions for common biochemical reagents like PBS, HEPES buffer, and Tris-HCl continue to see growing adoption over powder or concentrated formats in labs prioritizing convenience and reproducibility over reagent cost per use.
<a href="https://mbpinc.net/contact-us">Reach out to the friendly team at MBP</a> now to get a quote for your particular research needs.

Chemicals

Coomassie Brilliant Blue R-250

Unit Quantity

Shipping condition

<h2 dir="ltr">What are specimen collection swabs?</h2>
 Specimen collection swabs are sterile, single-use devices with a fiber tip on a plastic or wire shaft, used to acquire material from a patient's nasopharynx, anterior nares, oropharynx, wound, skin, or environment for diagnostic testing or microbial culture. Modern flocked swabs replace yards of wrapped fiber with perpendicular nylon or polyester fibers attached to the applicator, giving roughly 97 % specimen release on vortex versus about 33 % for traditional rayon or polyester. Buy from this category whenever specimen acquisition feeds a downstream culture or PCR &mdash; the swab determines whether the assay sees the analyte at all. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Sterile flocked swabs for high-efficiency sample collection and release</li>
<li dir="ltr" role="presentation">Polyester and rayon swabs for general microbiology sampling</li>
<li dir="ltr" role="presentation">Nylon flocked nasopharyngeal and/or oropharyngeal swabs</li>
<li dir="ltr" role="presentation">Dry swabs for direct plating or molecular testing workflows</li>
<li dir="ltr" role="presentation">Swabs with transport tubes for stabilized sample transfer</li>
<li dir="ltr" role="presentation">Breakpoint swabs for easy release into collection vials</li>
</ul>
<h2 dir="ltr"> How to choose specimen collection swabs</h2>
&nbsp;
Match the collection site Nasopharyngeal swabs use long flexible shafts (~150 mm) and small flocked or molded tips designed to reach the posterior nasopharynx. Anterior nares swabs are shorter and gentler. Oropharyngeal and wound swabs come in foam or flocked variants depending on the target organism.
Choose tip material for the assay Flocked nylon or polyester is the default for respiratory virus PCR. Cotton, calcium alginate, and wood-shafted swabs are excluded by CDC guidance because cotton contains PCR-inhibitory fatty acids and calcium alginate inhibits PCR directly. Foam suits some bacterial cultures and rapid antigen tests.
Verify the breakpoint and the shaft A predetermined breakpoint at a defined distance from the tip (commonly 100&ndash;101 mm for nasopharyngeal swabs) lets the operator snap the shaft into the transport tube; shafts should be plastic or wire, never wood.
Confirm sterility and FDA listing Single-use sterile peel pouches are the clinical standard. Swabs are FDA Class I exempt devices; verify the manufacturer's FDA registration and listing on the certificate of conformity.
<h2 dir="ltr"> Specifications context</h2>
 Diagnostic swabs are characterized by tip material, shaft material, total length, breakpoint distance, and sterility status. Nasopharyngeal flocked swabs commonly measure about 150 mm overall with a breakpoint near 100 mm and a flexible plastic shaft; the MedSchenker CavSwab uses Allium&trade; flock technology on a 153 mm shaft with a 101.3 mm breakpoint. Specimen release ranges from roughly 33 % for traditional rayon/polyester up to about 97 % for flocked nylon. As of 2026, these sit in a low-to-mid price tier per swab and are typically sold in case quantities of 100 or 500.&nbsp; 
&nbsp;
For swab selection, material compatibility, and sample collection formats,<a href="https://mbpinc.net/contact-us"> the MBP team&nbsp;</a>can guide you to the most suitable option based on your testing method and workflow setup.

Swabs

Copan FLOQSwabs

502CS01 by COPAN Diagnostics — individually sterile nylon flocked swab with 80mm breakpoint. Perpendicular fiber tip design releases more than 90% of collected analytes into elution media. Qualified for molecular assays, bacteriology, rapid antigen testing, DFA, EIA, cytology, and virology culture. 100 swabs per pack; 1,000 per case.

Single Regular Size Nylon Flocked Swab with 80mm Breakpoint in Peel Pouch

Single Flexible Minitip Size Nylon Flocked Swab with 100mm BP

Single Minitip Size Nylon Flocked Swab with 80mm

Single Regular Size Nylon Flocked Swab with 100mm Breakpoint in Peel Pouch – Individually Packaged, Sterile

Single Regular Size Nylon Flocked Swab with 30mm Breakpoint in Peel Pouch – Individually Packaged, Sterile (Package: 1000 units)

Cover Glass

<h2 data-section-id="smj2kt" data-start="0" data-end="23">What Is Cover Glass?</h2>
&nbsp;
Cover glass (also called coverslips or cover slips) are ultra-thin glass slips placed over a specimen mounted on a microscope slide. They may be rectangular, square, or circular depending on the application. Their main function is to protect the specimen from contamination and physical damage while also creating a uniform optical surface for high-quality imaging.
A critical factor in cover glass selection is thickness, since microscope objectives are optically corrected for specific coverslip standards. The two most common grades are No. 1 (nominal 150 &micro;m, range 130&ndash;170 &micro;m) and No. 1.5 (nominal 175 &micro;m, &plusmn;15 &micro;m). In most modern microscopy systems, No. 1.5 cover glass is preferred, especially for high-numerical aperture (high-NA) objectives used in fluorescence and confocal microscopy. Using the wrong thickness can introduce spherical aberration and reduce image sharpness.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Square coverslips for standard slide preparations and routine pathology.
</li>
<li dir="ltr" aria-level="1">
Rectangular cover glass for large tissue sections and smears.
</li>
<li dir="ltr" aria-level="1">
Circular glass coverslips for specialized cell culture and imaging.
</li>
<li dir="ltr" aria-level="1">
No. 1 thickness slips optimized for most microscope objectives.
</li>
<li dir="ltr" aria-level="1">
No. 1.5 high-precision glass for high-numerical aperture lenses.
</li>
<li dir="ltr" aria-level="1">
Pre-cleaned lint-free glass to eliminate imaging artifacts.
</li>
</ul>
&nbsp;
<h2 data-section-id="5ihghg" data-start="910" data-end="938">How to Choose Cover Glass</h2>
&nbsp;
Thickness: No. 1 vs No. 1.5
No. 1.5 cover glass (175 &micro;m nominal) is the standard choice for most modern objectives because it matches the optical correction of high-NA lenses used in advanced imaging. No. 1 cover glass (150 &micro;m nominal) should only be used when the microscope objective is specifically designed or labeled for that thickness. Mismatching cover glass thickness and objective correction leads to image distortion and reduced resolution, particularly at higher magnifications.
Shape: Square, Rectangular, or Circular
Square coverslips (commonly 18 &times; 18 mm or 22 &times; 22 mm) are used for routine microscopy and tissue sectioning. Rectangular coverslips (such as 24 &times; 50 mm or 24 &times; 60 mm) are preferred for larger specimens like extended tissue sections or blood smears. Circular coverslips are used in specialized applications such as glass-bottom culture dishes and live-cell imaging systems.
Surface Quality: Standard vs Pre-Cleaned vs Low-Autofluorescence
Standard cover glass may require cleaning before use, while pre-cleaned coverslips are ready for immediate mounting and help reduce particulate artifacts that interfere with imaging. For fluorescence and advanced imaging applications, low-autofluorescence or precision-grade cover glass is preferred because it reduces background signal and improves contrast and signal-to-noise ratio.
Quantity and Packaging
Cover glass is typically supplied in packs based on count or weight, with common packaging sizes ranging from 100 to 500 pieces per box. High-throughput laboratories often purchase in case quantities for cost efficiency and consistent supply management. MBP can assist with selecting appropriate pack sizes based on laboratory workflow and volume requirements.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3288" data-end="3313">Specifications Context</h2>
&nbsp;
Cover glass thickness is defined by industry standards rather than exact uniform measurement: No. 1 typically ranges from 130&ndash;170 &micro;m (nominal 150 &micro;m), while No. 1.5 typically ranges from 160&ndash;190 &micro;m (nominal 175 &micro;m). High-precision No. 1.5H cover glass offers tighter tolerances (approximately &plusmn;5 &micro;m) and is used in high-resolution confocal and super-resolution microscopy where optical consistency is critical. Standard borosilicate cover glass has a refractive index of approximately 1.515&ndash;1.523, which matches the correction assumptions of most microscope objectives.
Ready to see the difference? <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Contact the MBP Team</a> for a quote right now.

Datsheet

Well Cell Culture Plates

Cover glass,100/cs

801010 by NEST Scientific is a 14 mm round glass coverslip, 0.16–0.19 mm thick, sized for individual wells of standard 24-well culture plates. Non-cytotoxic, autoclavable, and resistant to organic solvents; manufactured in an ISO 7 cleanroom. For confocal, DIC, and fluorescence microscopy. Supplied 100/cs.

Cover glass,φ14mm, 100/cs

Cover glass,φ15mm, 100/cs

Cover glass,φ18mm, 100/cs

Cover glass,φ20mm, 100/cs

Cover glass,φ25mm, 100/cs

<h2>What are epigenetics enzymes and inhibitors?</h2>
&nbsp;
Epigenetics enzymes and inhibitors are the molecular tools for directly studying or modulating the enzymatic machinery that writes, erases, or reads epigenetic marks. The major classes include: DNA methyltransferases (DNMT1, DNMT3A, DNMT3B), which catalyze the addition of a methyl group to cytosine; TET family enzymes (TET1, TET2, TET3), which oxidize 5-methylcytosine to 5-hydroxymethylcytosine; histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups from histone lysine residues; and histone methyltransferases (HMTs) including EZH2, DOT1L, and G9a. Small-molecule inhibitors of HDAC and DNMT enzymes are the most clinically and experimentally advanced of these tools.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Micrococcal nuclease for nucleosome mapping and controlled chromatin digestion applications</li>
<li dir="ltr" role="presentation">DNA shearing enzymes for consistent fragmentation of DNA in sequencing and epigenetic workflows</li>
<li dir="ltr" role="presentation">DNA degradation enzymes for controlled breakdown and analysis of nucleic acids&nbsp;</li>
<li dir="ltr" role="presentation">CpG methylase enzymes for targeted DNA methylation (CpG-specific )&nbsp; in epigenetic modification studies</li>
<li dir="ltr" role="presentation">5-hmC glucosyltransferase for detection and labeling of hydroxymethylcytosine in DNA</li>
<li dir="ltr" role="presentation">SAM (S-adenosylmethionine) cofactors for supporting enzyme activity and methylation reactions</li>
<li dir="ltr" role="presentation">DNA digestion enzymes for genomic DNA processing workflows</li>
<li dir="ltr" role="presentation">dsDNA nuclease systems for controlled cleavage and analysis of double-stranded DNA</li>
</ul>
&nbsp;
<h2>How to choose epigenetics enzymes and inhibitors</h2>
&nbsp;
Choose DNMT inhibitors to globally reduce or deplete DNA methylation in cells
Nucleoside analog DNMT inhibitors like 5-azacytidine and decitabine incorporate into DNA and trap DNMT1, preventing maintenance methylation and causing passive demethylation through DNA replication, while newer non-nucleoside DNMT inhibitors are in development for more selective, non-genotoxic methylation reduction.
Choose HDAC inhibitors to globally increase histone acetylation
HDAC inhibitors including pan-HDAC hydroxamates like vorinostat (SAHA) or class-selective compounds reduce histone deacetylase activity and cause hyperacetylation of histone lysine residues, broadly promoting gene expression at silenced loci. HDAC inhibitors are the most clinically advanced class of epigenetic therapeutics, with multiple FDA-approved compounds.
Use TET enzyme preparations for in vitro 5-hmC oxidation studies
Purified TET1, TET2, or TET3 enzyme preparations allow controlled in vitro oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products, useful for generating defined 5-hmC-containing substrate DNA or studying TET enzyme activity and selectivity.
Choose EZH2 or other HMT inhibitors for studying PRC2-dependent gene silencing
EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), catalyzes H3K27 trimethylation (H3K27me3), a histone modification associated with repression of tumor suppressor genes, and EZH2 inhibitors like tazemetostat are used in research to probe the role of this silencing mark.
Combine inhibitors to study epistatic relationships between epigenetic marks
Because DNA methylation, histone acetylation, and histone methylation are mechanistically linked, combining HDAC inhibitors with DNMT inhibitors can produce synergistic effects on gene expression exceeding either alone, and these combination approaches are both experimental tools and the basis for combination epigenetic therapy research.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
HDAC and DNMT inhibitors are the most clinically advanced classes of epigenetic therapeutics, with multiple HDAC inhibitors including vorinostat and romidepsin approved for hematological malignancies, EZH2 inhibitors including tazemetostat having received accelerated approval, and multiple BET inhibitors in clinical trials. As of 2026, CRISPR/dCas9 systems fused with DNMTs, TETs, and HDACs enable locus-specific epigenome editing, extending classic enzyme and inhibitor approaches toward targeted epigenetic modulation.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and find the suitable enzymes and inhibitory reagents for your lab.

Enzymes & Inhibitors

Protocol

CpG Methylase (M. Sssl)

CpG Methylase (M. SssI) (E2010) by Zymo Research — recombinant CpG methyltransferase that completely methylates all 5′-CpG-3′ sites in dsDNA. Supplied with 10X buffer and 20X SAM. Available in 200 U and 400 U; optimal at 30°C; heat-inactivatable at 65°C.

Additional Information

Assay Condition

Heat Inactivation

Source

Storage

Unit Definition

CpG Methylase (M. Sssl) (200 U)

CpG Methylase (M. Sssl) (400 U)

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
<li dir="ltr" aria-level="1">
Targeted fluorescent labeling of specific cellular structures using viral delivery.
</li>
<li dir="ltr" aria-level="1">
Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

Cre Adenovirus (P1 Bacteriophage)

Vector

Cre Lentiviral Vector (Enterobacteria phage P1) (pLenti-III)

Cre-GFP Adenovirus

References

Cryo Boxes

<h2>What Are Cryo Boxes?</h2>
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Cryo boxes are lidded, gridded storage containers designed to organize cryogenic vials, microcentrifuge tubes, and centrifuge tubes inside ultra-low temperature (ULT) freezers at &minus;80&deg;C, vapor-phase liquid nitrogen refrigerators (&minus;150 to &minus;190&deg;C), and cryogenic dewars. The two primary materials are water-resistant coated cardboard (economical, single-use) and rigid polycarbonate or polypropylene (reusable, autoclavable, transparent). Standard well configurations&mdash;25 (5&times;5), 81 (9&times;9), and 100 (10&times;10)&mdash;in 2-inch and 3-inch box heights match common cryogenic vial sizes. The alphanumeric grid on the lid helps with sample tracking in LIMS systems. Cardboard cryo boxes are vapor-phase LN2 rated only; polycarbonate is required for autoclave use or liquid-phase LN2 immersion.
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<h2>What you will find:</h2>
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&bull; Standard cryo boxes for cryogenic vial storage &bull; Freezer storage boxes for ultra low temperature use &bull; Cardboard cryo boxes for cost effective storage &bull; Polypropylene cryo boxes for durability and reuse &bull; Grid format boxes for easy sample organization &bull; Hinged lid cryo boxes for quick access &bull; Color coded storage boxes for sample identification &bull; Cryo storage boxes for 1.5 mL and 2.0 mL vials
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<h2>How to Choose Cryo Boxes</h2>
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Material: Cardboard vs. Polycarbonate vs. Polypropylene
Cardboard cryo boxes are economical and used for long-term storage at &minus;80&deg;C or vapor-phase LN2. They are single-use and not autoclavable. Polycarbonate (PC) boxes are durable, transparent, and autoclavable at 121&deg;C, making them suitable for frequent handling. Polypropylene (PP) boxes also tolerate autoclaving and offer higher chemical resistance than PC. Choice depends on reuse frequency and sterilization needs.
Well Count and Vial Size
81-well (9&times;9) boxes fit standard 1.8&ndash;2.0 mL cryogenic vials up to ~13 mm outer diameter. 100-well (10&times;10) boxes are used for smaller 1.2&ndash;1.5 mL vials and microcentrifuge tubes up to ~12 mm OD. Always match vial diameter with box well size before ordering.
Box Height: 2-Inch vs. 3-Inch
2-inch boxes are standard for 1&ndash;2 mL cryovials and are most commonly used in biobanking. 3-inch boxes are for larger 3&ndash;5 mL vials and certain centrifuge tubes where extra depth is required.
LN2 Phase Compatibility
Both cardboard and hard-wall boxes can be used in vapor-phase LN2. Only polycarbonate or polypropylene boxes should be used for liquid-phase LN2 because cardboard can break down when submerged.
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<h2>Specifications Context</h2>
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Cryo boxes are typically rated from &minus;196&deg;C to +121&deg;C depending on material. Cardboard is suitable for vapor-phase LN2 only, while hard-wall plastic boxes can handle more demanding workflows. Proper selection depends on vial size, storage temperature, and how frequently samples are accessed.
Eliminate the risk of thermal damage. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Connect with the experts at Molecular Biology Products Inc.</a> to find the perfect storage solution for your high-value specimens.

DataSheet

Cryo Box

Cryo Box, 2.0 mL, 10*10, New Model, 1/pk, 12/cs

Cryo Box, 4.0 mL, 10*10, New Model, 1/pk, 8/cs

Cryo Box, 5.0 mL, 10*10, New Model, 1/pk, 6/cs

Cryo Box, 1.5 mL, 10*10, New Model, 1/pk, 14/cs

Cryo Box for Brooks, 8*6, 1/pk, 10/cs

Nest Scientific 616931 — 8×6 (48-position) cryogenic storage box for Brooks automated systems; PC lid / ABS base; -196°C to +70°C rated; compatible with SBS 2D-barcoded cryovials; 1/pk, 10/case.

Cryo Box for Brooks 1.9ml Cryovials 2D barcode on bottom 1D barcode on side (item # 612041 612047 and 612051), 8*6, 1/pk, 10/cs

Cryo Box for Brooks 4.0ml Cryovials 2D barcode on bottom 1D barcode on side (item # 614041 614047 and 614051), 8*6, 1/pk, 10/cs

United States

Texas

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