Brands

Centrifuge Tubes

<h2>What Are Centrifuge Tubes?</h2>
 Centrifuge tubes are cylindrical laboratory containers with caps that are used to separate, pellet, and store biological or chemical samples through centrifugal force. They are commonly made from polypropylene (PP) or polystyrene (PS) and are available in sizes ranging from small 0.5 mL microtubes to larger 50 mL conical tubes. These tubes are widely used for cell pelleting, protein precipitation, DNA and RNA extraction, tissue homogenate clarification, and long-term sample storage. Depending on the material, they can withstand temperatures from &minus;80 &deg;C to +121 &deg;C.
In short, the best centrifuge tube depends on your centrifuge rotor, required g-force, sample volume, and sterility needs. 
<h2>What You Will Find: </h2>
<ul>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes" title="All Centrifuge Tubes">All Centrifuge Tubes</a>: Comprehensive selection of 15 mL and 50 mL conical tubes with clear graduations for daily lab workflows.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/oak-ridge-style-centrifuge-tubes" title="Oak Ridge Style Centrifuge Tubes">Oak Ridge Style Centrifuge Tubes</a>: High-speed, chemical-resistant tubes designed to withstand extreme RCF without deformation.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/centrifuge-bottles" title="Centrifuge Bottles">Centrifuge Bottles</a>: Heavy-duty, larger-capacity vessels engineered for bulk sample processing and high-velocity runs.</li>
<li><a href="https://mbpinc.net/consumables/centrifuge-tubes/large-volume-centrifuge-tubes" title="Large Volume Centrifuge Tubes">Large Volume Centrifuge Tubes</a>: Industrial-strength 250 mL and 500 mL tubes optimized for pilot-scale harvesting and bioprocessing.</li>
</ul>
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<h2>How to Choose Centrifuge Tubes</h2>
 Volume and Rotor Compatibility
Select a tube size that matches both your sample volume and centrifuge rotor. Microtubes ranging from 0.5&ndash;2 mL are commonly used in microcentrifuges and can typically handle speeds up to 20,000 &times; g. Midi tubes in the 5&ndash;10 mL range are suitable for intermediate sample volumes, while 15 mL and 50 mL conical tubes are commonly used in clinical, research, and preparative centrifuges.
Material: Polypropylene vs. Polystyrene
Polypropylene (PP) tubes offer excellent chemical resistance and can tolerate temperatures from &minus;80 &deg;C to +121 &deg;C. They are suitable for autoclaving and exposure to many laboratory solvents. Polystyrene (PS) tubes provide superior optical clarity, making it easier to visually inspect samples and liquid levels, but they have lower resistance to solvents and extreme temperatures.
Sterility and Contamination Control
Sterile centrifuge tubes are typically gamma- or E-beam-irradiated and certified free of RNase, DNase, and pyrogens. These tubes are recommended for RNA extraction, cell culture, and clinical applications where contamination must be minimized. Non-sterile tubes may still be RNase- and DNase-free and are often suitable for routine DNA work and general sample storage.
Cap Design
Attached screw caps help prevent cap mix-ups and sample contamination, especially in high-throughput laboratories. Separate caps can be quicker to open and may be preferred in workflows that require frequent access to samples. If tubes will be used with automated systems, cap dimensions should be verified for compatibility with liquid handling equipment.
Packaging Options
Racked tubes provide convenient organization and allow direct pipetting while the tubes remain in the rack. Bulk-bagged tubes offer a more economical option for laboratories that process large volumes of samples and already have tube storage systems in place.
<h2> Comparing Common Centrifuge Tube Types</h2>
 Microtubes (0.5&ndash;2 mL) are ideal for PCR preparation, microcentrifuge applications, and storage of small sample volumes. They can generally withstand centrifugal forces up to 20,000 &times; g.
Midi tubes (5&ndash;10 mL) are designed for intermediate sample volumes such as culture supernatants and can typically tolerate forces up to 16,000 &times; g.
Sterile 15 mL conical tubes are frequently used for cell pelleting, RNA extraction, and clinical sample processing, with typical ratings between 12,000 and 15,000 &times; g.
Sterile 50 mL conical tubes are commonly selected for larger-volume applications including tissue culture work, density gradient preparation, and large-scale sample processing.
Polystyrene conical tubes in 15 mL and 50 mL formats are often chosen when visual sample inspection is important and solvent exposure is minimal.
<h2> Specifications and Features</h2>
 Polypropylene centrifuge tubes from Globe Scientific and Nest Scientific are manufactured from USP Class VI virgin resin and do not contain plasticizers or processing additives. Sterile products are typically irradiated to achieve a sterility assurance level (SAL) of 10⁻⁶. Most 15 mL tubes feature graduation marks at 1 mL intervals, while 50 mL tubes are usually graduated every 5 mL. MBP offers both bagged and racked centrifuge tube formats, including 15 mL and 50 mL conical tubes as well as smaller microtube and midi tube options.
MBP supports procurement departments by accepting purchase orders and offering institutional pricing for qualified high-volume customers. <a href="https://mbpinc.net/contact-us">Contact the MBP team today</a> for a customized quote.

<h2 data-section-id="155qizo" data-start="0" data-end="33">What Are All Centrifuge Tubes?</h2>
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The All Centrifuge Tubes collection includes the complete range of tube sizes commonly used in research, clinical, and laboratory environments. These tubes range from small 0.5 mL and 1.5&ndash;2 mL microtubes used in microcentrifuge applications to 5 mL and 10 mL midi tubes for intermediate sample volumes, as well as 15 mL and 50 mL conical tubes designed for cell culture, density gradient preparation, and larger-volume sample processing. They are available in polypropylene (PP) for enhanced chemical resistance and temperature tolerance or polystyrene (PS) for improved optical clarity.
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What you will find:
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/microcentrifuge-tubes" title="Microcentrifuge Tubes">Microcentrifuge Tubes</a> &ndash; Precision-engineered for small-volume sample preparation.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/screw-cap-tubes" title="Screw Cap Tubes">Screw Cap Tubes</a> &ndash; Secure, leak-proof closures for long-term storage and transport.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/15ml-and-50ml-centrifuge-tubes" title="15ml &amp; 50ml Centrifuge Tubes">15ml &amp; 50ml Centrifuge Tubes</a> &ndash; Standard conical bottom tubes for large-volume processing.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/midicentrifuge-tubes-5ml-and-10ml" title="Midicentrifuge Tubes (5ml &amp; 10ml)">Midicentrifuge Tubes (5ml &amp; 10ml)</a> &ndash; The perfect bridge for intermediate sample sizes.
</li>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/consumables/centrifuge-tubes/all-centrifuge-tubes/microtubes-box" title="Microtubes Box">Microtubes Box</a> &ndash; Durable storage racks for organized sample management and protection.
</li>
</ul>
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<h2 data-section-id="1f93yys" data-start="914" data-end="963">How to Choose From All Centrifuge Tube Formats</h2>
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Volume and Rotor Compatibility
Selecting the correct tube size is essential for safe and efficient centrifugation. Microtubes ranging from 0.5&ndash;2 mL are designed for microcentrifuges, while 5&ndash;10 mL midi tubes fit compatible benchtop centrifuge rotors. Larger 15 mL and 50 mL conical tubes are commonly used in clinical and research centrifuges. Always verify rotor compatibility before introducing a new tube format into your workflow.
Sterility Requirements
For sensitive applications such as RNA extraction, cell culture, and clinical diagnostics, sterile tubes certified free of RNase, DNase, and pyrogens are recommended. Non-sterile tubes can provide a cost-effective solution for routine DNA analysis, protein assays, and general sample storage where sterility is not critical.
Material Selection
Polypropylene tubes offer excellent durability, can be autoclaved at 121 &deg;C, and resist many laboratory chemicals and solvents. Polystyrene tubes provide superior transparency, making it easier to monitor liquid levels and sample contents, although they are less resistant to heat and solvents.
Packaging and Inventory Planning
Microtubes are commonly available in pack sizes ranging from 500 to 1,000 units, while 15 mL and 50 mL conical tubes are often supplied in cases of 500. Laboratories with consistent usage may benefit from establishing recurring purchase orders to ensure uninterrupted supply.
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<h2 data-section-id="urfo3f" data-start="2395" data-end="2433">Compare Centrifuge Tube Size Groups</h2>
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Microtubes (0.5&ndash;2 mL) are primarily used for PCR preparation, microcentrifuge applications, and storage of small sample volumes. These are typically manufactured from polypropylene and are available in pack sizes of 500&ndash;1,000 units.
Midi tubes (5&ndash;10 mL) support intermediate-volume centrifugation and supernatant collection. They are commonly made from polypropylene and supplied in packs ranging from 100&ndash;500 units.
15 mL conical tubes are widely used for cell pelleting, RNA isolation, and clinical sample processing. They are available in both polypropylene and polystyrene formats and are generally packaged in cases of 500.
50 mL conical tubes are designed for larger-volume workflows, including tissue culture, density gradient preparation, and sample processing. Like 15 mL tubes, they are available in polypropylene and polystyrene and are commonly sold in cases of 500.
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<h2 data-section-id="1ezckh3" data-start="3318" data-end="3348">Specifications and Features</h2>
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Globe Scientific and Nest Scientific centrifuge tubes are manufactured from USP Class VI virgin polypropylene or polystyrene resin and are certified free from detectable RNase, DNase, and pyrogens. Sterile products are irradiated using gamma or E-beam methods to achieve a sterility assurance level (SAL) of 10⁻⁶. MBP offers both racked and bagged formats for 15 mL and 50 mL conical tubes, along with dispenser-box options for selected microtube product lines that support efficient use in high-throughput laboratory environments.
For procurement teams, MBP accepts purchase orders and offers institutional pricing for bulk and high-volume purchases. <a href="https://mbpinc.net/contact-ushttps://mbpinc.net/contact-us" title="Contact the MBP team" style="color: #236fa1;">Contact the MBP team</a> today to learn more

All Centrifuge Tubes

<h2 data-section-id="kx7ycn" data-start="0" data-end="39">What Are Screw Cap Centrifuge Tubes?</h2>
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Screw cap centrifuge tubes are polypropylene vessels with a threaded closure that rotates to create a compression seal, typically reinforced by a silicone or polyethylene O-ring. This seal design prevents evaporation, leakage, and contamination under conditions where a snap cap fails, including high-speed centrifugation up to 20,000 &times; g, repeated freeze-thaw cycles from &minus;80 &deg;C to +121 &deg;C, exposure to volatile organic solvents, and long-term cryogenic storage. Available in volumes ranging from 0.5 mL to 2.0 mL, these tubes are commonly used in proteomics, genomics, drug discovery, biobanking, and other applications where maintaining sample integrity is critical throughout multiple processing steps.
Choose screw cap tubes instead of snap-cap tubes when working with volatile samples, repeated freeze-thaw cycles, high centrifugal forces above 16,000 &times; g, or long-term storage at &minus;80 &deg;C.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">In this part, you will discover:</h2>
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<ul>
<li dir="ltr" aria-level="1">
0.5mL, 1.5mL, 2.0mL (for small to moderate volume applications)
</li>
</ul>
<ul>
<li dir="ltr" aria-level="1">
O-ring caps: Leak-proof, prevents evaporation
</li>
<li dir="ltr" aria-level="1">
Tubes only: Compatible with existing caps
</li>
</ul>
<ul>
<li dir="ltr" aria-level="1">
Sterile: For sensitive, contamination-prone work
</li>
<li dir="ltr" aria-level="1">
Non-sterile: Cost-effective for routine use
</li>
</ul>
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<h2 data-section-id="1w7d404" data-start="949" data-end="981">How to Choose Screw Cap Tubes</h2>
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O-Ring vs Non-O-Ring Seal
Screw cap tubes with integrated silicone O-rings provide a hermetic seal that is ideal for volatile chemicals, infectious materials, and valuable samples where evaporative loss must be minimized. Non-O-ring designs, including rim-seal and plug-seal caps, are suitable for most routine molecular biology storage applications at temperatures between &minus;20 &deg;C and &minus;80 &deg;C and are often easier to open when working in cold environments.
Internal Thread vs External Thread
Internal-thread screw cap tubes maintain a smooth exterior profile and are generally compatible with standard microcentrifuge rotors without requiring additional adapters. External-thread designs provide easier cap handling while wearing gloves and are frequently used in self-standing tube formats that can be placed directly in racks.
Volume Selection: 0.5 mL vs 1.5 mL vs 2.0 mL
0.5 mL screw cap tubes are commonly used for concentrated enzymes, antibodies, and other valuable reagents where minimizing dead volume is important.
1.5 mL screw cap tubes are the standard choice for molecular biology applications, including RNA and DNA storage, pellet recovery, and protein fraction collection.
2.0 mL screw cap tubes provide additional capacity and headspace for bead-based extraction methods, precipitation workflows, and sample mixing procedures.
Nest Scientific offers 0.5 mL, 1.5 mL, and 2.0 mL self-standing screw cap tubes with multiple cap color options to simplify sample identification and organization.
Sterility Grade
Sterile screw cap tubes are gamma- or E-beam-irradiated to a sterility assurance level (SAL) of 10⁻⁶ and are recommended for RNA applications, clinical specimen processing, and cell culture workflows. Non-sterile RNase-free and DNase-free tubes provide a cost-effective alternative for DNA and protein-related applications.
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<h2 data-section-id="ibka1i" data-start="2868" data-end="2901">Compare Screw Cap Tube Options</h2>
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Nest Scientific self-standing screw cap tubes are available in 0.5 mL, 1.5 mL, and 2.0 mL volumes with external threads and integrated sealing rings. Rated up to 20,000 &times; g, these tubes are ideal for general sample storage and laboratories that use color-coded organization systems.
Globe Scientific self-standing screw cap tubes with silicone O-rings are offered in 1.5 mL and 2.0 mL formats. Their enhanced sealing performance makes them particularly suitable for volatile samples, cryogenic storage, and applications where maximum sample protection is required. These tubes are rated up to 20,000 &times; g.
Conical-bottom screw cap tubes are commonly available in 1.5 mL formats with internal threads and optional sealing systems. Their conical design improves pellet recovery and makes them well suited for precipitation and centrifugation workflows. These tubes can withstand centrifugal forces up to 20,000 &times; g.
Amber screw cap tubes are available in 1.5 mL and 2.0 mL capacities and feature O-ring seals for additional protection. Designed for light-sensitive samples, they are typically rated up to 16,000 &times; g and help protect reagents from light-induced degradation.
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<h2 data-section-id="1jl751w" data-start="4094" data-end="4119">Specifications Context</h2>
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Globe Scientific and Nest Scientific screw cap microtubes are manufactured from USP Class VI virgin polypropylene and are certified RNase-free, DNase-free, and non-pyrogenic by lot. Nest Scientific screw cap tubes are available with six cap color options, including blue, red, yellow, purple, green, and natural, and are typically packaged in packs of 50 tubes with 500 tubes per case. These tubes are suitable for temperatures ranging from &minus;80 &deg;C to +121 &deg;C.
As of 2026, MBP stocks both self-standing and conical-bottom screw cap tube formats from Globe Scientific and Nest Scientific. Availability may vary by volume, cap style, and color configuration.
Don't let equipment choices hold back your next breakthrough. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Contact our team at Molecular Biology Products Inc.</a> for a custom quote.

Screw Cap Tubes

Data Sheet-SCT-050SS-C-S

Data Sheet-SCT-150-C-S

Data Sheet-SCT-150SS-C-S

Clear Tube with Screw Caps, Preassembled, Sterile

SCT-050SS-C-S Group by Novas Bio — sterile self-standing screw-cap tubes in 0.5 mL, 1.5 mL, and 2.0 mL formats. ANSI/AAMI/ISO 11137, SAL 10⁻⁶, endotoxin/RNase/DNase/BSE-TSE-free.

Manufacturer

Screw Cap Tube. 0.5ml. Self-Standing. Sterile. Preassembled.

Screw Cap Tube. 1.5ml. Conical. Sterile. Preassembled.

Screw Cap Tube. 1.5ml. Self-Standing. Sterile. Preassembled.

Screw Cap Tube. 2.0ml. Self-Standing. Sterile. Preassembled.

Sample Storage Container

<h2 dir="ltr">What are Urine Collection Products?</h2>
 Urine collection products for laboratory use are containers and preservative systems designed to collect, stabilize, and transport urine specimens for urinalysis (UA), urine culture (C&amp;S), nucleic acid-based testing (NAAT for STIs), cell-free DNA (cfDNA) liquid biopsy, and 24-hour timed collections. Formats include sterile cups (30&ndash;60 mL), preservative tubes (5&ndash;50 mL), vacuum-draw urine transfer tubes, 24-hour collection containers (2&ndash;3 L), and preservative-prefilled devices for ambient-temperature stabilization of DNA, RNA, and proteins. Choose urine collection products with an active preservative when specimens cannot be processed within 2 hours of collection, when samples must be shipped to a centralized lab, or when nucleic acid integrity over 24+ hours is required.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Sterile urine collection containers for routine diagnostic sampling</li>
<li dir="ltr" role="presentation">Urine transport tubes for secure handling and transfer workflows</li>
<li dir="ltr" role="presentation">Preservative urine tubes for culture and urinalysis stability</li>
<li dir="ltr" role="presentation">Midstream urine collection systems for contamination reduction</li>
<li dir="ltr" role="presentation">24-hour urine collection containers for metabolic testing workflows</li>
<li dir="ltr" role="presentation">Leak-resistant screw-cap urine tubes for safe transport</li>
<li dir="ltr" role="presentation">Graduated urine containers with volume markings for easy measurement</li>
<li dir="ltr" role="presentation">Pediatric urine collection solutions for low-volume sampling</li>
<li dir="ltr" role="presentation">Closed-system urine transfer devices for reduced exposure handling</li>
<li dir="ltr" role="presentation">General laboratory urine collection consumables for research use</li>
</ul>
<h2 dir="ltr"> How to Choose Urine Collection Products</h2>
 Application: Culture vs. NAAT vs. Nucleic Acid Research For culture (C&amp;S), use sterile cups or boric acid preservative tubes. For NAAT (STI panels for Chlamydia, Gonorrhea), use sterile cups or manufacturer-validated transfer tubes; confirm with your platform's IFU (e.g., Hologic Aptima Combo 2 uses a specific urine transport tube). For nucleic acid research (cfDNA, RNA, protein), use dedicated urine preservative tubes (Norgen, Streck) that stabilize analytes for up to 72 hours at room temperature. Volume: Random vs. 24-Hour Collection Random midstream urine (10&ndash;30 mL) is adequate for urinalysis, culture, and NAAT. Timed 24-hour collections (total volume 500&ndash;3000 mL) require large-volume containers with the appropriate preservative for the ordered panel. Mixing 24-hour containers with different preservatives is a common error&mdash;verify the correct preservative type with the laboratory before dispensing to the patient. Preservative Compatibility Boric acid tubes maintain bacterial counts for culture up to 24 hours at room temperature. Boric acid is NOT compatible with NAAT workflows&mdash;it inhibits PCR. For NAAT, use plain sterile containers or manufacturer-specific transfer tubes. For liquid biopsy cfDNA, Streck Cell-Free DNA Urine Preservative maintains cell integrity and prevents genomic DNA contamination for up to 72 hours. Sterility All urine containers intended for culture must be certified sterile. Containers for NAAT must be certified DNase-free and RNase-free to prevent nucleic acid contamination from the container. Confirm sterility and nuclease-free certification in the manufacturer's datasheet before use in molecular workflows. Automated Compatibility For high-throughput labs using automated urine analyzers (Sysmex UF-5000, Siemens CLINITEK), confirm that the collection tube format and cap design are compatible with tube-handling robotics. Copan UriSponge&reg; (FDA-cleared October 2024) is a boric-acid-free device validated for direct loading on WASP&reg; automation systems. 
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<h2 dir="ltr">Specifications Context</h2>
 Standard urine collection cups are manufactured from transparent polypropylene (PP) or polystyrene (PS) with wide-mouth, screw-cap lids. Sterile cups rated for culture meet ISO 7886 and are non-pyrogenic. Boric acid preservative tubes contain approximately 1.8% boric acid, stabilizing bacterial counts by creating a bacteriostatic environment. Norgen urine preservative tubes (5&ndash;50 mL, available in multiple sizes) stabilize DNA, RNA, microRNA, and protein from fresh urine for over 2 years at room temperature per manufacturer validation data. As of 2026, liquid biopsy studies using urine cfDNA routinely use Streck Cell-Free DNA Urine Preservative (3 mL per 30 mL urine) as the standard stabilization protocol in published clinical research protocols. 
Need help picking the right urine collection setup? <a href="https://mbpinc.net/contact-us">Contact MBP</a>, and we&rsquo;ll help you sort it out fast. No confusion, just the right fit for your workflow.

Urine Collection Products

5574-Analyzer Consumables Catalog

Coagulation Cup with Metal Mixing Bar, PS, for use with the Accustasis, CoaData and BFT2 analyzers, BAG/1000

Globe Scientific 5574 — polystyrene coagulation cuvette with metal mixing bar for the Accustasis®, CoaData®, and BFT2® analyzers. Sold in bags of 1,000. Suited for PT, INR, aPTT, and fibrinogen assays in clinical coagulation laboratories.

Manufacture

<h2>What are 5-hmC ELISA kits?</h2>
&nbsp;
5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
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<h2>How to use 5-hmC ELISA kits</h2>
&nbsp;
Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

MSDS

Coating Buffer

D5425-1-15 by Zymo Research — Coating Buffer, 15 mL; component of the Quest 5-hmC DNA ELISA Kit; used in the microplate coating step for 5-hydroxymethylcytosine (5-hmC) detection and quantification. For research use only.

Shipping Condition

Storage Condition

Coating Buffer (15 ml)

Coating Buffer (30 ml)

Cuvettes

<h2 dir="ltr">What are Cuvettes?</h2>
 Cuvettes are small, precision-dimensioned transparent vessels used to hold liquid samples in the light path of a spectrophotometer, fluorometer, or colorimeter for measurement of absorbance, transmittance, fluorescence intensity, or fluorescence polarization. The standard cuvette has a 10 mm optical path length (the distance light travels through the sample), a square or rectangular cross-section, and an internal volume of 1&ndash;4 mL. Cuvettes are manufactured in four main materials&mdash;polystyrene (PS) or polymethylmethacrylate (PMMA) plastic, optical glass, UV-grade quartz (fused silica), and IR quartz&mdash;each covering a different wavelength range. Choose plastic cuvettes for routine visible-light absorbance (380&ndash;780 nm) where disposability prevents cross-contamination; choose optical glass for reusable visible-range work (340&ndash;2,500 nm); choose UV quartz when measurements extend into the UV range below 340 nm (190&ndash;2,500 nm).
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1cg0wqn" data-start="1810" data-end="1862">Spectrophotometer cuvettes for absorbance analysis</li>
<li data-section-id="vbobpt" data-start="1863" data-end="1890">Polystyrene (PS) cuvettes</li>
<li data-section-id="14z5a6i" data-start="1891" data-end="1906">PMMA cuvettes</li>
<li data-section-id="1h4vr7o" data-start="1907" data-end="1966">Analyzer-specific cuvettes for clinical chemistry systems</li>
<li data-section-id="b3k6fl" data-start="1967" data-end="1998">Standard path length cuvettes</li>
<li data-section-id="td8a19" data-start="1999" data-end="2043">Accessories for spectrophotometer cuvettes</li>
</ul>
<h2 dir="ltr"> How to Choose Cuvettes</h2>
 Material and Wavelength Range Plastic (PS/PMMA) cuvettes transmit visible light from 380&ndash;780 nm and are single-use, eliminating cleaning and cross-contamination risk. They are unsuitable for UV measurements, organic solvents, or strong acids. Optical glass cuvettes transmit 340&ndash;2,500 nm, covering the full visible and near-infrared range; Different manufacturers recommend different procedures. UV quartz cuvettes are traditionally used for DNA/RNA quantification at OD260/OD280 because they transmit UV wavelengths below 340 nm. IR quartz extends to 3,500 nm for near-IR applications. Optical Path Length The standard 10 mm path length follows the Beer-Lambert law at normal sample concentrations. Semi-micro (5 mm path) and micro-volume cuvettes (1 mm path, or internal volumes down to 10&ndash;70 &micro;L) are used for precious or limited-volume samples. The path length directly multiplies absorbance: a 1 mm cuvette gives 1/10 the absorbance of a 10 mm cuvette for the same solution &mdash; account for this in Beer-Lambert calculations. Volume: Macro vs. Semi-Micro vs. Micro Macro cuvettes (1&ndash;4 mL) are the standard for routine spectrophotometry. Semi-micro cuvettes (0.5&ndash;1.5 mL) reduce sample consumption. Micro cuvettes (10&ndash;350 &micro;L) are used when sample volume is limited; sub-micro cuvettes (10 &micro;L or less) suit precious clinical or research samples. Confirm that your spectrophotometer's beam height and aperture are compatible with the cuvette format before purchasing micro formats. Two-Window vs. Four-Window Standard cuvettes have two optically clear windows (the light-entry and exit faces) and two frosted or ribbed side walls for gripping. Four-window (all-clear) cuvettes are required for fluorescence measurements at 90&deg; to the excitation beam &mdash; the two additional clear windows allow emitted fluorescence to exit perpendicular to the excitation path. Confirm whether your instrument requires a standard or fluorescence-grade cuvette. Reusable vs. Disposable Quartz and glass cuvettes are reusable; their higher up-front cost&nbsp;is justified for low-throughput precision work or when solvents or UV wavelengths are required. Plastic cuvettes cost fractions of a cent per use and eliminate washing time in high-throughput assay workflows. Select disposable plastic for any routine colorimetric assay (Bradford, BCA, ELISA plate reader backup, OD600 bacterial growth) where wavelengths stay in the visible range. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
The 10 mm path length standard cuvette has internal dimensions of 10 &times; 10 mm cross-section and an internal height of approximately 45 mm. Outer dimensions are 12.5 &times; 12.5 mm, fitting all standard spectrophotometer cell holders. Window parallelism tolerance is &plusmn;0.01 mm for precision quartz cuvettes; standard optical glass cuvettes have &plusmn;0.1 mm tolerance. Light transmission through UV quartz: &gt;90% at 220 nm and &gt;85% at 200 nm. For nucleic acid quantification, a UV quartz cuvette with a minimum path length of 10 mm is required for OD 260/280 measurements; the ratio of OD260/OD280 should be 1.8&ndash;2.0 for pure DNA and 1.9&ndash;2.1 for pure RNA as a purity indicator. As of 2026, plastic disposable cuvettes remain the most cost-efficient choice for labs running &gt;50 absorbance readings per day in the visible range.
&nbsp;
Need the right fit? MBP can guide you to cuvettes that match your workflow perfectly. For institutional pricing and PO-based bulk ordering, <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">contact MBP</a>&nbsp;directly.

5120-Group-Analyzer Consumables Catalog

Cobas Mira Cuvettes

Globe Scientific 5120 are PMMA cuvette segments compatible with Cobas Mira®, Mira S®, Mira Plus®, and Horiba ABX Mira Plus® analyzers. 12-place configuration, individually wrapped, 50 per box, 500 per case. Third-party compatible replacement, not OEM.

COBAS MIRA: Cuvette, for use with Cobas Mira, Mira S, Mira Plus and Horiba ABX Mira Plus analyzers, Individually Wrapped, 50/Box, 10 Boxes/Unit, CS/500

COBAS MIRA: Cuvette, for use with Cobas Mira, Mira S, Mira Plus and Horiba ABX Mira Plus analyzers, Individually Wrapped, 50/Box, 6 Boxes/Unit, CS/300

COBAS MIRA: Cuvette, Racked, for use with Cobas Mira, Mira S, Mira Plus and Horiba ABX changer analyzers, 15/Rack, 30 Racks/Unit, CS/450

Plates

Collection Plate (2 Plates)

C2002 by Zymo Research — 96-well round-bottom collection plate; 1.2 mL per well; clear polypropylene; compatible with Silicon-A, Zymo-Spin I-96, IB-96, and III-96 plates; sold as 2 plates per pack.

Spin Columns

Collection/Filter Assemblies

C1040-5 Collection/Filter Assemblies by Zymo Research are Zymo-Spin III-P column assemblies with 15 mL conical and 50 mL reservoirs for large-volume lysate processing in DNA and RNA purification workflows. 5 assemblies per pack.

Binding Capacity

Compatibility

Elution Volume

Format

Matrix/Construction

Volumetric

Zymo-Spin™ III-P Column Assembly w/ 15 ml and 50 ml Reservoir (5 Pack)

Zymo-Spin™ III-S Columns w/ LuerLock Caps and 15 ml Reservoir (25 Pack)

Nitrile Gloves

<h2 data-section-id="upfogw" data-start="0" data-end="38">What are nitrile laboratory gloves?</h2>
&nbsp;
Nitrile laboratory gloves are single-use disposable hand protection items made from nitrile butadiene rubber (NBR), a fully synthetic material that contains no natural rubber proteins. Because of this, they are inherently latex-free and are widely used as the standard glove type in modern research, clinical, pharmaceutical, and diagnostic laboratories.
They are designed to provide a strong balance between chemical resistance, puncture resistance, and tactile sensitivity. Compared to natural rubber latex, nitrile gloves are typically around three times more resistant to punctures at the same thickness. Most nitrile gloves used in laboratories are medical-grade with AQL &le; 2.5, meaning they meet strict quality control limits for defects and are suitable for regulated environments. ASTM D6319 defines their performance requirements, including minimum tensile strength, elongation, and barrier integrity.
&nbsp;
<h2 data-path-to-node="2">What you will find:<a href="https://mbpinc.net/consumables/ppe/gloves/nitrile-gloves"></a></h2>
&nbsp;
<ul data-path-to-node="3">
<li>
Powder-free nitrile gloves for clean laboratory use and reduced particulate risk
</li>
<li>
Examination-grade nitrile gloves for research, diagnostics, and clinical applications
</li>
<li>
Medical-grade nitrile gloves for highly sensitive and sterile applications
</li>
<li>
Blue nitrile gloves for standard lab workflows and easy visibility
</li>
<li>
Black nitrile gloves for contamination-sensitive tasks and masking stains
</li>
</ul>
&nbsp;
<h2 data-section-id="1apuf9x" data-start="1144" data-end="1186">How to choose nitrile laboratory gloves</h2>
&nbsp;
Thickness and application
Nitrile gloves come in different thicknesses depending on the task. Thinner gloves (around 3 mil) are used for high-dexterity work such as pipetting, PCR setup, and delicate cell culture tasks where precision is important. Medium thickness gloves (4&ndash;5 mil) are the standard choice for general laboratory work, clinical diagnostics, and pharmaceutical handling because they balance protection and flexibility. Thicker gloves (6 mil and above) are used when handling stronger chemicals, hazardous reagents, or when additional puncture resistance is needed.
AQL grade
AQL (Acceptable Quality Level) indicates defect rate. Medical-grade nitrile gloves typically have an AQL of 2.5 or lower and are required for work involving biological samples, pathogens, or pharmaceutical materials. Industrial-grade gloves with higher AQL values are not suitable for regulated laboratory or clinical use.
Color selection
Nitrile gloves are available in different colors such as blue, black, and purple. Blue is the most common because it provides strong visibility and easy detection of tears or contamination. Black gloves are often used in industrial or chemical environments, while purple gloves are sometimes used in clinical settings to differentiate them from latex products. Color does not affect chemical protection or performance at the same thickness.
Chemotherapy and hazardous drug resistance
Some nitrile gloves are specifically tested under ASTM D6978 for resistance to chemotherapy drugs and hazardous pharmaceuticals. These are required in oncology, compounding pharmacies, and laboratories handling cytotoxic agents. Standard nitrile gloves without this certification should not be assumed to provide protection against these compounds.
Chemical resistance limits
Nitrile gloves provide good resistance to oils, alcohols, dilute acids, and many aqueous solutions. However, they are not suitable for strong solvents such as acetone, toluene, chloroform, or other highly aggressive organic chemicals. For those, specialized glove materials must be selected based on chemical compatibility charts.
&nbsp;
<h2 data-section-id="1fljvas" data-start="4128" data-end="4153">Specifications context</h2>
&nbsp;
Nitrile examination gloves are governed by ASTM D6319, which defines mechanical strength, elongation, and quality standards for medical-grade nitrile products. FDA-regulated exam gloves require 510(k) clearance for clinical use. Standard packaging typically includes 100 gloves per box and 1,000 gloves per case. As of 2026, 4&ndash;5 mil powder-free blue nitrile gloves with AQL 2.5 remain the most widely used glove type across global research and pharmaceutical laboratories due to their balance of safety, durability, and chemical resistance.
Choose MBP nitrile gloves to maintain safety, precision, and confidence in every task you handle. <a href="https://mbpinc.net/contact-us">Reach out to the MBP team</a> for a personalized quote and keep your laboratory protected with the best in class.

Colloidal Oatmeal Coated Latex Gloves Specifications

Colloidal Oatmeal Coated Latex Gloves

The 903011 by NEST Scientific is a size Small powder-free nitrile examination glove with a patented colloidal oatmeal coating recognized by the FDA as a skin protectant. Latex-free nitrile construction; 0.07 mm palm thickness; chlorinated, ambidextrous, beaded cuff. Non-sterile. 100/pk, 1000/cs. Medium (903021) and Large (903031) also available.

Nitrile gloves with oats extractions, powder free, a patented coating recognized by the FDA as a skin protectant, S, 100/pk, 1000/cs

Nitrile gloves with oats extractions, powder free, a patented coating recognized by the FDA as a skin protectant, M, 100/pk, 1000/cs

Nitrile gloves with oats extractions, powder free, a patented coating recognized by the FDA as a skin protectant, L, 100/pk, 1000/cs

Latex Gloves

<h2>What are latex examination gloves?</h2>
&nbsp;
Latex examination gloves are single-use disposable hand protection items made from natural rubber latex (NRL), which is derived from the sap of the rubber tree (Hevea brasiliensis). They are designed to meet ASTM D3578 standards for strength, elasticity, fit, and barrier integrity.
Compared with synthetic alternatives like nitrile, latex gloves have a lower elastic stiffness, meaning they stretch more easily and conform closely to the shape of the hand. This gives them superior tactile sensitivity, which is why they are still widely used in precision laboratory work, dental procedures, surgical environments, and fine sample manipulation tasks. Most modern latex gloves are powder-free, using chlorination or polymer coatings to allow easy donning without contamination risks.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>
Powder-free latex gloves for reduced contamination risk and cleaner samples
</li>
<li>
Examination-grade latex gloves for versatile laboratory use and diagnostics
</li>
<li>
High-dexterity latex gloves for precise handling during intricate tasks
</li>
</ul>
&nbsp;
<h2>How to choose latex examination gloves</h2>
&nbsp;
Tensile strength and elongation
Latex gloves are tested under ASTM D3578, which requires high elasticity and durability. They must withstand significant stretching without tearing, ensuring reliability during pipetting, dissection, and clinical procedures. This high elongation capacity is one of the main performance advantages of latex over nitrile.
AQL and medical-grade standards
Medical-grade latex gloves typically have an AQL of 2.5 or lower, meaning very low acceptable defect rates. These are required for laboratory, clinical, and pharmaceutical applications involving biological or chemical exposure. Industrial-grade gloves with higher AQL values are not suitable for controlled or regulated environments.
Powder-free requirement
All laboratory latex gloves should be powder-free. Powdered versions are no longer appropriate for scientific or clinical use because they can contaminate cell cultures, PCR reactions, and sensitive analytical assays. Powder-free gloves use chlorination or polymer coatings instead of cornstarch powder.
Grip and surface texture
Latex gloves may be smooth or textured. Textured fingertips improve grip during wet procedures such as pipetting, centrifuge tube handling, or tissue manipulation. Smooth versions are easier to don but offer less grip in wet environments.
Latex allergy risk
Natural rubber latex contains allergenic proteins that can trigger reactions in sensitive individuals. Because of this, laboratories must assess allergy risk and ensure nitrile alternatives are available. Repeated exposure increases sensitization risk, so many facilities have shifted fully to nitrile.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Latex examination gloves are governed by ASTM D3578, which defines mechanical strength, elongation, and quality requirements. Additional standards such as ASTM D5712 and ASTM D6124 assess protein content and residual powder levels. Medical-grade latex gloves are regulated as Class II medical devices in many regions and require compliance documentation such as FDA 510(k) clearance for clinical use. As of 2026, powder-free latex gloves remain common in surgical, dental, and high-dexterity laboratory applications, although many general labs now prefer nitrile due to allergy considerations.
If you're looking to balance precision with protection, MBP can help you choose the right latex glove option for your lab needs. <a href="https://mbpinc.net/contact-us">Connect with our helpful team</a> today to request a quote.

904031 by NEST Scientific is a powder-free natural rubber latex examination glove, size Large, 0.07mm (2.76 mil) thick, ASTM D3578 compliant, with an FDA-recognized colloidal oatmeal interior coating that reduces skin dryness and irritation during extended glove wear, supplied non-sterile, 100/pk, 1,000/cs, for laboratory, clinical, and high-frequency-use environments.

Latex Gloves with Oats Extractions, Powder Free, a patented coating recognized by the FDA as a skin protectant, L, 100/pk, 1000/cs

Latex Gloves with Oats Extractions, Powder Free, a patented coating recognized by the FDA as a skin protectant, M, 100/pk, 1000/cs

Latex Gloves with Oats Extractions, Powder Free, a patented coating recognized by the FDA as a skin protectant, S, 100/pk, 1000/cs

<h2>What are microbial and environmental DNA isolation kits?</h2>
&nbsp;
Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
&nbsp;
<h2>How to choose a microbial and environmental DNA isolation kit</h2>
&nbsp;
Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

DataSheet

Column-Pure Animal Genomic DNA Isolation Kit

Unit Quantity

Application

Column-Pure Bacterial Genomic DNA Isolation Kit

<h2>What Are Spin-Column Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
A spin-column genomic DNA kit for blood and tissue combines a proteinase K-based direct lysis step with a silica-membrane spin column to purify genomic, mitochondrial, or viral DNA from animal blood, tissue, and cultured cells without phenol, chloroform, or alcohol precipitation. Related entities include direct-lysis protocols, dried blood spot supplementary workflows, TissueLyser mechanical disruption, and downstream PCR, genotyping, and sequencing library preparation.&nbsp;Use spin-column formats for individual or small-batch genomic DNA extraction from blood, cells, or tissue using a standard microcentrifuge.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Efficient isolation of high-quality genomic DNA from blood, saliva, tissues, and other biological samples using rapid spin-column purification.&nbsp;</li>
<li>Maximize DNA recovery with optimized lysis and binding chemistry while minimizing inhibitor, protein, and contaminant interference</li>
<li>Midiprep, microprep, and miniprep formats available to support varied sample input volumes&nbsp;</li>
<li>User-friendly workflows and streamlined protocols with fast processing times for efficient genomic DNA extraction&nbsp;</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Blood/Tissue Genomic DNA Kit</h2>
&nbsp;
Lysis Approach by Sample Type
Whole blood, buffy coat, buccal cells, and cultured cells generally undergo direct proteinase K lysis without mechanical disruption. In contrast, fibrous or fatty tissues such as muscle, liver, or skin often require mechanical homogenization prior to the lysis step to ensure complete cell disruption.
Anticoagulant and Blood Sample Handling
These kits typically support fresh or frozen blood collected in EDTA, citrate, or heparin. Dried blood spots generally require supplementary protocols and additional equipment beyond standard spin-column workflows.
Column Elution Chemistry
Elution is typically performed using Tris-based buffer or nuclease-free water, with volumes ranging from approximately 50&ndash;200 &micro;L depending on kit design and sample type. Water elution may be preferred for EDTA-sensitive downstream applications.
Scaling Beyond a Single Column
For higher throughput workflows, 96-well plate formats using the same silica-membrane chemistry enable simultaneous processing of multiple samples per centrifuge run. Spin-column formats remain best suited for method development and low-to-moderate sample volumes.
&nbsp;
<h2>Specifications Context:</h2>
&nbsp;
The most important parameters for spin-column blood/tissue genomic DNA kits are sample-type compatibility and elution buffer composition, as these determine whether additional pretreatment is required and whether the eluate is directly compatible with downstream workflows. A typical 200 &micro;L whole blood input yields low-microgram quantities of genomic DNA in an elution volume of approximately 50&ndash;200 &micro;L.
&nbsp;
Use MBP's <a href="mbpinc.net/quickorder">Quick Order tool</a> for bulk pricing or <a href="mbpinc.net/contact-us">contact the specialist team</a> to confirm pretreatment requirements for challenging tissue types.