Brands

Sample Cups

<h2 dir="ltr">What are Automation Sample Cups?</h2>
 Automation sample cups (also called analyzer sample cups, autosampler cups, or J-cups) are small-volume polystyrene or polypropylene vessels designed to hold aliquoted patient specimens for direct loading into automated clinical chemistry analyzers, immunoassay platforms, hematology analyzers, and ICP-MS/ICP-OES spectrometers. They are used when an analyzer's primary tube handler cannot directly aspirate from a standard 15 mL or 50 mL collection tube&mdash;typically because the sample volume is insufficient, a diluted aliquot is required, or a specific cup geometry is mandated by the instrument's sampling needle. Globe Scientific offers the largest selection of alternate (OEM-equivalent) sample cups for laboratory analyzers, providing significant per-cup savings versus manufacturer-supplied consumables. Choose analyzer sample cups by instrument model first&mdash;dimensional compatibility is non-negotiable&mdash;then optimize for material (PS for optical clarity, PP for chemical resistance) and volume.
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<h2 dir="ltr">What you will find:</h2>
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0.2 mL automation sample cups for micro-volume testing
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0.5 mL sample cups for routine analyzer use
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1.5 mL sample cups for standard clinical workflows
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Clear polystyrene sample cups for accurate readings
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Conical-bottom cups for complete sample pickup
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Low-dead-volume cups for minimal sample waste
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Analyzer-compatible sample cups for clinical systems
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Disposable sample cups for single-use workflows
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Nesting cups for easy storage and handling
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</ul>
<h2 dir="ltr"> How to Choose Automation Sample Cups</h2>
 Instrument Compatibility: Dimensional Match is Mandatory Automation sample cups must match the exact outer diameter, height, and cup-bottom geometry specified by the instrument manufacturer. A cup that is 0.5 mm too wide or too narrow will not seat correctly in the autosampler rotor, causing aspiration failures, pipetting errors, or instrument alarms. Globe Scientific engineers and validates each alternate cup to OEM specifications for named analyzer platforms; confirm the instrument model against the catalog's compatibility list before ordering. Volume: 0.5 mL vs. 1.5 mL vs. 2.0 mL 0.5 mL cups (13.55 &times; 24.50 mm, polystyrene) are used on chemistry analyzers including Beckman CX series, Alpha Wassermann, Bio-Chem Labs, and DPC Immulite platforms. 1.5 mL cups (13.8 &times; 22.60 mm) are used on BioMerieux AG-2/ASCA and MLA 1600C platforms. Larger 2.0 mL cups are used on Roche cobas and Horiba ABX platforms. The minimum working volume of the cup must exceed the analyzer's dead volume (typically 150&ndash;400 &micro;L) plus the required sample volume. Material: Polystyrene vs. Polypropylene Polystyrene (PS) sample cups provide excellent optical clarity for colorimetric-based analyzers that photometrically read the cup contents and have low protein adsorption for routine chemistry analytes. Polypropylene (PP) cups are used when the sample contains organic solvents, strong acids (trace element analysis), or when acid-wash protocols are required for ICP-MS autosampler systems. MoldPro and Globe Scientific PP autosampler cups for PerkinElmer, Agilent, and Shimadzu ICP systems are manufactured from virgin PP with ultra-low metal content verified by ICP-MS lot testing. With or Without Caps/Inserts Sample cups with caps are used when the aliquot must be sealed to prevent evaporation or cross-contamination during batch loading. Insert cups (smaller inner cup that sits inside a standard tube) allow aliquoted samples to be loaded directly without relabeling &mdash; a common efficiency gain in high-volume core labs. Confirm whether your instrument's autosampler accepts capped cups or requires open-top formats. ICP-MS vs. Clinical Chemistry Applications ICP-MS autosampler cups require certified ultra-low metal content (verified by ICP-MS at ppb level), manufactured from virgin PP without metal-containing additives, dyes, or fillers. Standard PS clinical chemistry cups contain low-level metal additives from colorants and should not be used for trace element or heavy metal analysis. Always use metal-free certified cups for any elemental analysis application. 
<h2 dir="ltr"> Specifications Context</h2>
 Globe Scientific analyzer sample cups are manufactured to exact OEM specifications and provide equivalent precision and optical properties to original manufacturer cups. PS cups have optical clarity with &ge;90% light transmission in the visible spectrum, required for photometric analyzers. PP ICP-MS autosampler cups meet ultra-low metal content specifications: typically &lt;1 ppb for Fe, Zn, Cu, Pb, Mn, and other analytes commonly measured in trace element panels. Cup dimensions are held to &plusmn;0.1 mm tolerance on O.D. to ensure consistent seating in autosampler rotors. As of 2026, Globe Scientific is the most widely stocked alternate analyzer consumable brand through lab distributors, including MBP, offering per-cup savings of 20&ndash;40% versus OEM pricing for high-volume clinical labs. 
<a href="https://mbpinc.net/contact-us">Contact MBP&nbsp;</a>for per-cup pricing on case-quantity PO orders.

Automation Sample Cups

113132-113133-Analyzer Consumables Catalog

Caps for Sample Cups

113132 by Globe Scientific is a polyethylene (PE) snap cap for Globe multi-purpose sample cups 110610, 110021, 110711, and 110621. The solid-top design snaps firmly in place to contain specimens during collection and transport. Sold in bags of 1000, suited to high-throughput drug testing and clinical specimen workflows.

Manufacture

Cap, Snap, PE, for Sample Cups: 110610, 110021, 110711 & 110621, BAG/1000

Cap, Snap, PE, with Pierceable Cross Cut, for Sample Cups: 110021, 110610, 110621 & 110711, BAG/1000

<h2 data-section-id="1go10h8" data-start="173" data-end="215">What are classic cell culture media?</h2>
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Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture since the mid-20th century: DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's Nutrient Mixtures F-10 and F-12, IMDM (Iscove's Modified Dulbecco's Medium), and the combination formulation DMEM/F-12. Each is manufactured according to its originally published formula, with modifications limited to those necessary for consistent performance and stability of the medium over its shelf life. These media supply the amino acids, vitamins, glucose, salts, and (in most formulations) phenol red pH indicator that form the nutritional backbone of essentially all subsequent specialty, serum-free, and Advanced media derivatives.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Selective Mammalian Antibiotics including Puromycin, Geneticin, and Hygromycin B for stable development of cell lines.</li>
<li dir="ltr" role="presentation">Broad-Spectrum Bactericidal Agents for routine contamination prevention, including Penicillin/Streptomycin and Gentamicin&nbsp;</li>
<li dir="ltr" role="presentation">Prokaryotic Selection Reagents for effective bacterial transformation, including Ampicillin, Kanamycin, Chloramphenicol, and Tetracycline&nbsp;</li>
<li dir="ltr" role="presentation">Antifungal and Antimycotic Solutions to maintain sterile cultures, including combined 100X cocktails and Amphotericin B (Fungizone)&nbsp;</li>
<li dir="ltr" role="presentation">Specialized Small Molecules for targeted biochemical assays, including Ganciclovir, Rapamycin, and Actinomycin D&nbsp;</li>
</ul>
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<h2 data-section-id="1mfajxk" data-start="1218" data-end="1252">How to choose a classic medium</h2>
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DMEM vs MEM
DMEM contains up to four times the amino acid and vitamin concentration of original Eagle's MEM, along with higher glucose levels, making it suitable for cell types with elevated nutrient demands &mdash; DMEM is broadly applicable across mammalian, insect, and plant cell phenotypes including primary cells, stem cells, and transformed lines. MEM remains widely used for monolayer culture of many standard cell types, with optional Hanks' or Earle's salt formulations and a calcium-free modification that permits suspension growth.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640, developed at the Roswell Park Memorial Institute for culturing human leukemic cells, is optimized for non-adherent cell types &mdash; lymphocytes and other blood cells &mdash; and contains glutathione plus higher biotin, B12, and PABA concentrations than DMEM or MEM. Despite its hematopoietic origin, RPMI 1640 supports a broad range of adherent lines (HeLa, T47D, RENCA, CT26.WT) when properly supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were originally developed for CHO cell clones, HeLa cells, and mouse L-cells, and remain suited to hepatocyte culture, viral fusion experiments, and toxicity assays, with formulations available for use with or without serum. Coon's modification of Ham's F-12 roughly doubles the amino acid and pyruvate content and adds ascorbic acid, developed specifically for hybrid cells produced by viral fusion.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 (1:1 mixture) combines DMEM's glucose, amino acid, and vitamin richness with Ham's F-12's zinc, putrescine, hypoxanthine, and thymidine, producing an extremely rich complex medium used for MDCK cells, glial cells, fibroblasts, and human endothelial cells &mdash; and serving as the basal medium for many serum-free Advanced formulations used in organoid and stem cell work.
IMDM for high-density rapid proliferation
IMDM further enriches DMEM with additional amino acids and vitamins, substituting potassium nitrate for ferric nitrate and adding HEPES and sodium pyruvate. IMDM's sodium bicarbonate buffer system (5&ndash;10% CO₂) and enriched composition make it well suited for rapidly proliferating, high-density cultures including Jurkat, COS-7, and macrophage cells.
Liquid vs. powder format
Liquid classic media are ready to use (after appropriate FBS and supplement addition) and include sodium bicarbonate in the formulation. Powdered media are manufactured without sodium bicarbonate, since it tends to off-gas in powder form &mdash; 3.7 g/L sodium bicarbonate must be added upon reconstitution, followed by sterile filtration. Powder formats offer cost and shipping-weight advantages for high-volume labs preparing their own media.
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<h2 data-section-id="17i0pj" data-start="5035" data-end="5061">Specifications context</h2>
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Classic media are manufactured according to originally published formulas, with stability-related modifications documented per product. DMEM's sodium bicarbonate buffer system at 3.7 g/L is standard across liquid formulations; powdered formats require this addition upon reconstitution along with 0.22 &micro;m sterile filtration. As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the three most widely specified classic media across academic and biomanufacturing labs, with IMDM increasingly used for high-density suspension applications in bioprocessing.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents (GlutaMAX, NEAA, sodium pyruvate, HEPES), see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get highly efficient microbial control solutions for your lab.

Antibiotics

MSDS

Carbenicillin

Unit Quantity

Concentration

Recommended Protocol

Storage Condition

Shipping condition

Carboys

<h2 data-section-id="1pn5j3m" data-start="0" data-end="31">What Are Laboratory Carboys?</h2>
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Laboratory carboys are large-volume plastic containers&mdash;10 L, 20 L, and 50 L being the standard sizes&mdash;used for bulk storage, transport, and dispensing of DI water, buffers, culture media, reagents, and liquid waste. They are manufactured from polypropylene (PP, autoclavable at 121&deg;C) or HDPE (broad chemical resistance, rated to &minus;100&deg;C) with integrated handles, molded liter/gallon graduations, and screw caps with PP integral-seal closures. Optional spigots enable gravity-controlled dispensing without lifting. The rectangular profile of modern carboys maximizes shelf density compared to round containers of the same volume. Globe Scientific EZgrip carboys are molded from USP Class VI, FDA-grade resins&mdash;PP for autoclavable applications and HDPE for chemical and cold storage. PP carboys are the correct choice for autoclave sterilization; cap must be loosened before every autoclave cycle to prevent collapse during cooling.
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<h2 dir="ltr">What you will find:</h2>
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Small carboys (4&ndash;8 L) for bench-top use and daily workflows
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Medium carboys (10&ndash;20 L) for routine liquid storage
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Large carboys (20&ndash;50 L+) for bulk storage applications
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Polypropylene (PP) carboys for autoclavable use
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HDPE carboys for chemical-resistant storage
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Carboys with spigots for easy dispensing
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<h2 data-section-id="og787b" data-start="962" data-end="997">How to Choose Laboratory Carboys</h2>
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Material: PP vs HDPE
Polypropylene is used when autoclave sterilization is required, as it tolerates 121&deg;C steam cycles with the cap loosened. PP also provides resistance to most acids, bases, and alcohols and offers partial translucency for content visibility. HDPE is not autoclavable at 121&deg;C but provides broad chemical resistance, low leachables, and performance down to &minus;100&deg;C, making it suitable for non-sterile chemical storage, waste collection, and cold buffer holding.
Volume
The 10 L carboy is used for media preparation batches, chromatography buffer reservoirs, and bench-scale waste collection. The 20 L carboy is standard for daily buffer preparation, fermentation media supply, and larger waste workflows. The 50 L carboy is reserved for high-throughput bioprocessing, large fermentation systems, and bulk waste accumulation where frequent emptying of smaller units is impractical.
Spigot vs No Spigot
Spigot-equipped carboys allow controlled gravity dispensing without lifting, reducing spill risk and handling strain. Non-spigot carboys are emptied by manual pouring and typically require a dedicated pouring cap for control. Spigots are recommended for 20 L and larger volumes due to handling safety and workflow efficiency.
Amber vs Natural
Natural (translucent) carboys are used for most standard buffers and reagents where visual level monitoring is sufficient. Amber HDPE carboys are used for light-sensitive reagents, biological media, and pharmaceutical intermediates where UV and visible light protection is required during storage.
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<h2 data-section-id="1jl751w" data-start="3200" data-end="3225">Specifications Context</h2>
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Globe Scientific EZgrip PP carboys are molded from USP Class VI, FDA-grade autoclavable polypropylene with integrated handles for safe lifting and transport. EZgrip HDPE carboys are produced from USP Class VI, FDA-grade HDPE with optional amber variants meeting USP &lt;671&gt; light transmission requirements. As of 2026, 20 L PP carboys with spigots are widely used in pharmaceutical pilot labs and academic biochemistry departments for sterile buffer preparation and chromatography feed supply systems. Explore jerricans for 5&ndash;20 L active transport containers and sample storage containers for smaller-volume applications.
<a href="https://mbpinc.net/contact-us">Connect with the MBP team today</a> to discuss your specific volume requirements and chemical compatibility, and find the perfect carboy for your laboratory&rsquo;s bulk storage needs.

Carboy, Autoclavable, with Handle , Non-Sterile

789011 by NEST is a 10-liter autoclavable USP Grade VI polypropylene carboy with handle, rated from -40 to 121 degrees C and lot-tested for pyrogens, cytotoxins, and DNase/RNase in an ISO 7 cleanroom. Non-sterile, no tap included. Supplied 6 per case with molded volume markings and a guaranteed leakproof design for culture media storage and buffer containment.

Manufacturer

10L Carboy, Autoclavable, with Handle ,without Tap, Non-Sterile, 6/cs

20L Carboy, Autoclavable, with Handle ,without Tap, Non-Sterile, 3/cs

10 L Carboy, Autoclavable, with Handle, with Tap, non-sterile, 4/cs

20 L Carboy, Autoclavable, with Handle, with Tap, non-sterile, 3/cs

Cryo Boxes

<h2>What Are Cryo Boxes?</h2>
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Cryo boxes are lidded, gridded storage containers designed to organize cryogenic vials, microcentrifuge tubes, and centrifuge tubes inside ultra-low temperature (ULT) freezers at &minus;80&deg;C, vapor-phase liquid nitrogen refrigerators (&minus;150 to &minus;190&deg;C), and cryogenic dewars. The two primary materials are water-resistant coated cardboard (economical, single-use) and rigid polycarbonate or polypropylene (reusable, autoclavable, transparent). Standard well configurations&mdash;25 (5&times;5), 81 (9&times;9), and 100 (10&times;10)&mdash;in 2-inch and 3-inch box heights match common cryogenic vial sizes. The alphanumeric grid on the lid helps with sample tracking in LIMS systems. Cardboard cryo boxes are vapor-phase LN2 rated only; polycarbonate is required for autoclave use or liquid-phase LN2 immersion.
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<h2>What you will find:</h2>
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&bull; Standard cryo boxes for cryogenic vial storage &bull; Freezer storage boxes for ultra low temperature use &bull; Cardboard cryo boxes for cost effective storage &bull; Polypropylene cryo boxes for durability and reuse &bull; Grid format boxes for easy sample organization &bull; Hinged lid cryo boxes for quick access &bull; Color coded storage boxes for sample identification &bull; Cryo storage boxes for 1.5 mL and 2.0 mL vials
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<h2>How to Choose Cryo Boxes</h2>
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Material: Cardboard vs. Polycarbonate vs. Polypropylene
Cardboard cryo boxes are economical and used for long-term storage at &minus;80&deg;C or vapor-phase LN2. They are single-use and not autoclavable. Polycarbonate (PC) boxes are durable, transparent, and autoclavable at 121&deg;C, making them suitable for frequent handling. Polypropylene (PP) boxes also tolerate autoclaving and offer higher chemical resistance than PC. Choice depends on reuse frequency and sterilization needs.
Well Count and Vial Size
81-well (9&times;9) boxes fit standard 1.8&ndash;2.0 mL cryogenic vials up to ~13 mm outer diameter. 100-well (10&times;10) boxes are used for smaller 1.2&ndash;1.5 mL vials and microcentrifuge tubes up to ~12 mm OD. Always match vial diameter with box well size before ordering.
Box Height: 2-Inch vs. 3-Inch
2-inch boxes are standard for 1&ndash;2 mL cryovials and are most commonly used in biobanking. 3-inch boxes are for larger 3&ndash;5 mL vials and certain centrifuge tubes where extra depth is required.
LN2 Phase Compatibility
Both cardboard and hard-wall boxes can be used in vapor-phase LN2. Only polycarbonate or polypropylene boxes should be used for liquid-phase LN2 because cardboard can break down when submerged.
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<h2>Specifications Context</h2>
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Cryo boxes are typically rated from &minus;196&deg;C to +121&deg;C depending on material. Cardboard is suitable for vapor-phase LN2 only, while hard-wall plastic boxes can handle more demanding workflows. Proper selection depends on vial size, storage temperature, and how frequently samples are accessed.
Eliminate the risk of thermal damage. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Connect with the experts at Molecular Biology Products Inc.</a> to find the perfect storage solution for your high-value specimens.

3090-1-Group-Cryogenic Vial Brochure

Cardboard Storage Boxes Specifications

Cardboard Storage Boxes

3090-1 by Globe Scientific is a 64-place (8×8) cardboard freezer storage box (134 × 134 × 47 mm) rated −196°C to +121°C, designed for 1.0–2.0 mL cryogenic vials. The liquid-resistant coating, drainage holes, numbered interior grid, and included dividers make it suitable for liquid nitrogen and −80°C mechanical freezer biobanking. Order via mbpinc.net/quickorder using catalog number 3090-1.

Freezing Box, 2", Cardboard, 64-Place (8x8 format), fits 1.0mL and 2.0mL CryoCLEAR vials, White, EA

Freezing Box, 2", Cardboard, 64-Place (8x8 format), fits 1.0mL and 2.0mL CryoCLEAR vials, White, CS/96

Freezing Box, 2", Cardboard, 81-Place (9x9 format), fits 1.0mL and 2.0mL CryoCLEAR vials, White, EA

Freezing Box, 2", Cardboard, 81-Place (9x9 format), fits 1.0mL and 2.0mL CryoCLEAR vials, White, CS/96

Freezing Box, 2", Cardboard, 81-Place (9x9 format), fits 1.0mL and 2.0mL CryoCLEAR tubes, Red, EA

Freezing Box, 2", Cardboard, 81-Place (9x9 format), fits 1.0mL and 2.0mL CryoCLEAR tubes, Red, CS/96

Freezing Box, 2", Cardboard, 100-Place (10x10 format), fits 1.0mL and 2.0mL CryoCLEAR vials, White, EA

Freezing Box, 2", Cardboard, 100-Place (10x10 format), fits 1.0mL and 2.0mL CryoCLEAR vials, White, CS/96

Freezing Box, 3", Cardboard, 64-Place (8x8 format), fits 3.0mL, 4.0mL and 5.0mL CryoCLEAR vials, White, EA

Freezing Box, 3", Cardboard, 64-Place (8x8 format), fits 3.0mL, 4.0mL and 5.0mL CryoCLEAR vials, White, CS/48

Freezing Box, 3", Cardboard, 64-Place (8x8 format), fits 3.0mL, 4.0mL and 5.0mL CryoCLEAR vials, Yellow, EA

Freezing Box, 3", Cardboard, 64-Place (8x8 format), fits 3.0mL, 4.0mL and 5.0mL CryoCLEAR vials, Yellow, CS/48

Freezing Box, 3", Cardboard, 81-Place (9x9 format), fits 3.0mL, 4.0mL and 5.0mL CryoCLEAR vials, White, EA

Freezing Box, 3", Cardboard, 81-Place (9x9 format), fits 3.0mL, 4.0mL and 5.0mL CryoCLEAR vials, White, CS/48

Freezing Box, 3", Cardboard, 100-Place (10x10 format), fits 3.0mL, 4.0mL and 5.0mL CryoCLEAR vials, White, EA

Freezing Box, 3", Cardboard, 100-Place (10x10 format), fits 3.0mL, 4.0mL and 5.0mL CryoCLEAR vials, White, CS/48

Freezing Box, 3", Cardboard, 36-Place (6x6 format), fits Diamond Midi 5.0mL Snap Cap and Screw Cap Tubes, White, CS/48

Freezing Box, Cardboard, 49-Place (7x7 format), for 15mL Centrifuge Tubes, White, EA

Freezing Box, Cardboard, 49-Place (7x7 format), for 15mL Centrifuge Tubes, White, CS/36

Freezing Box, Cardboard, 16-Place (4x4 format), for 50mL Centrifuge Tubes, White, EA

Freezing Box, Cardboard, 16-Place (4x4 format), for 50mL Centrifuge Tubes, White, CS/48

<h2 dir="ltr"> What Are Cas9 Enzymes?</h2>
 Cas9 is the catalytic core of the CRISPR/Cas9 editing system. Sourced from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9), recombinant Cas9 proteins are expressed and purified to high purity for direct use in ribonucleoprotein (RNP) complexes &mdash; currently the most widely recommended format for mammalian cell editing due to rapid activity onset and minimised off-target effects compared to plasmid-based delivery. Genome-editing performance depends on the appropriate nuclease format. Choose the Cas9 enzyme that aligns with your editing strategy.
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<h2 dir="ltr">What you will find:</h2>
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NLS-Tagged Cas9 and saCas9 Nucleases: Recombinant proteins of high purity designed for quick nuclear localization and strong initiation of double-strand breaks (DSBs) at specific genomic sites.
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Precision Cas9 Nickase (H840A): Tailored variants created for dual-nicking approaches to improve genomic stability and notably minimize off-target alterations.
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Lyophilized Nuclease Preparations: Nuclease-free, shelf-stable proteins that offer optimal adaptability for reconstitution and high-throughput automated CRISPR-related processes.
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Catalytically Inactive Null Mutants: "Dead" Cas9 (dCas9) proteins designed for DNA-binding research and specific epigenetic modification without changing the original sequence.
</li>
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Cas12a (asCpf1) Nucleases: Alternative CRISPR systems that focus on T-rich PAM sequences to broaden the range of spatial transcriptomics and targetable genomic areas.
</li>
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GFP-Tagged Cas9 Proteins: Fluorescently marked nucleases developed for real-time assessment of transfection efficacy and visual verification of cellular absorption.
</li>
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Endotoxin-Free Enzyme Formulations: Highly pure mixtures crucial for preserving cell viability in sensitive lentiviral applications and primary cell modifications.
</li>
</ul>
<h2 dir="ltr"> How to Choose a Cas9 Enzyme</h2>
 PAM Requirement Wild-type SpCas9 recognises any genomic locus flanked by an NGG protospacer adjacent motif (PAM), occurring roughly every 8&ndash;12 bp in mammalian genomes. SaCas9 requires NNGRRT, though engineered PAM variants (KKH, KRH, KRKH) expand targetable space. Off-Target Sensitivity High-fidelity Cas9 variants carry substitutions in the recognition lobe that reduce non-specific DNA contacts, lowering off-target cleavage while maintaining on-target activity comparable to wild-type SpCas9 &mdash; recommended for therapeutic cell engineering and base editing. Editing Mechanism Wild-type SpCas9 generates a blunt double-strand break repaired by NHEJ (indels for knockout) or HDR (precise insertions). Nickase (D10A) cuts only one strand for paired-nick HDR strategies with reduced off-target indels. dCas9 carries mutations in both nuclease domains, enabling CRISPRa/CRISPRi gene regulation without DNA cleavage. AAV Compatibility SaCas9, at approximately 1 kb smaller than SpCas9, fits a complete Cas9 + sgRNA expression cassette within the ~4.7 kb AAV packaging capacity &mdash; enabling single-vector in vivo delivery without split-vector complexity. Storage and Handling Recombinant Cas9 proteins are supplied for stability during shipping and short-term storage at &minus;20 &deg;C or &minus;80 &deg;C. Repeated freeze-thaw cycles reduce activity; aliquoting upon receipt is recommended.
<h2 dir="ltr"> Specifications Context</h2>
 An RNP is a pre-formed complex of recombinant Cas9 protein and sgRNA; once electroporated, it acts immediately and is degraded within hours, reducing off-target cleavage and avoiding immune stimulation associated with plasmid or mRNA delivery.&nbsp;Recombinant Cas9 in RNP format is validated across HEK293, HeLa, iPSCs, T cells, primary neurons, and hepatocytes, though electroporation parameters typically require cell-type-specific optimisation. Product offerings reflect MBP's catalogue as of mid-2026. 
&nbsp;
Reach out to the supportive <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">team at MBP Inc.</a> now to obtain a quotation for the perfect Cas9 enzyme for your study.

Cas9 Enzymes

DataSheet

Cas12a (asCpf1) Nuclease NLS Protein

Cas Type

Cas Origin

Cas Protein Marker

Format General

Storage Buffer

Caution

Shipping Condition

Cas12a (fnCpf1) Nuclease NLS Protein

Cas9 D10A Nickase GFP NLS Protein

<h2 dir="ltr">What Are Lentiviral &amp; Vector Systems for CRISPR?</h2>
 Lentiviral vector (LV) systems deliver CRISPR/Cas9 components with the capacity to carry complex transgenes and sustain robust, long-term expression across dividing and non-dividing cell types. Unlike transient transfection, lentiviral transduction integrates the transgene into the host genome, making it the method of choice for generating stable Cas9-expressing cell lines and pooled sgRNA library screens. The right vector system influences gene delivery efficiency, expression control, and experimental success. Select the platform that best fits your research objectives.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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Epigenetic Modulator Lentivirus (TET1CD/DNMT3A): Tailored viral platforms created for precise DNA methylation or demethylation to investigate gene control and epigenetic changes at particular sites.
</li>
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CRISPRa &amp; CRISPRi Delivery Mechanisms (VPR/KRAB): Advanced dCas9 lentiviral vectors designed for strong transcriptional enhancement or repression while preserving the original DNA sequence.
</li>
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Cas9 Nuclease &amp; Nickase Lentiviruses: Immediately usable viral particles that ensure effective transport of double-strand break or single-strand nicking enzymes for lasting site-directed mutagenesis.
</li>
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Lentiviral Cloning &amp; Expression Vectors: Adaptable transfection-ready genetic frameworks equipped with C-terminal tags and enhanced promoters for the creation of tailored guide RNA and effector proteins.
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Non-Viral Cas9 Vector Options: High-quality plasmid-based systems intended for temporary expression in scenarios where viral incorporation is not needed.
</li>
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High-Titer Packaging Solutions: Viral preparations safe from endotoxins, enhanced for optimal transduction efficiency in delicate therapeutic candidate evaluation and disease modeling.
</li>
</ul>
<h2 dir="ltr"> How to Choose Lentiviral/Vector CRISPR Systems</h2>
 Packaging Generation Second- and third-generation packaging designs progressively remove viral accessory genes (nef, vif, vpr, vpu) for enhanced biosafety. Third-generation systems split packaging components across two plasmids and eliminate the Tat transactivator, producing replication-incompetent particles suitable for BSL-2 settings. CRISPR-Specific Format Cas9 lentiviral plasmids provide stable Cas9 integration; pre-linearised sgRNA lentiviral plasmids are ready for guide sequence insertion; dox-inducible (Tet-On) Cas9 systems allow temporally controlled editing. Paired Cas9 and sgRNA vectors generate inducible stable editing cell lines. Transient vs. Stable Needs For experiments requiring transient editing without permanent genomic modification, all-in-one or dual-plasmid Cas9/sgRNA transfection systems (lipid-based or electroporation) are appropriate. For stable, heritable expression, lentiviral integration is required. Cell Type Lentiviral vectors transduce both dividing and non-dividing cells, including post-mitotic neurons and macrophages &mdash; applications that gamma-retroviral vectors cannot support. Screening Scale Pooled sgRNA libraries transduced at low MOI enable genome-wide functional screens; stable lentiviral Cas9 cell lines, generated once and banked, provide a consistent starting point for multiple guide RNA screen iterations. 
<h2 dir="ltr"> Specifications Context</h2>
 Lentiviral and vector systems support pooled genome-wide CRISPR knockout screens, stable Cas9 cell line generation, inducible gene editing models, hard-to-transfect primary cell engineering, and CAR-T cell development workflows. Lentiviral plasmids contain LTRs prone to recombination in standard E. coli strains &mdash; recombination-resistant strains such as Stbl3 are recommended for plasmid propagation. Integrase-deficient lentiviral vectors (IDLVs) or RNP electroporation are preferred where insertional mutagenesis risk must be minimised, such as therapeutic cell engineering. For research-scale work where stable integration is the goal, third-generation lentiviral systems remain the standard choice given their established safety record and broad cell-type compatibility across academic and industry labs. Labs new to lentiviral work often start with a well-characterised packaging system and a small pilot transduction before scaling to a full screen or stable line generation campaign.
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Reach out to the professional <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">team at MBP Inc.</a> now to obtain a quote for the perfect Lentivirus system tailored to your research needs.

Vector & Lentivirus Systems

Lentivirus Systems

Cas9 Double Mutant Lentiviral Vector

Vector

Cas9 Double Mutant Lentivirus

Titer

<h2 dir="ltr">What Are AAV and Adenovirus CRISPR Delivery Systems?</h2>
 AAV and adenovirus-based delivery systems exploit the natural cell-entry machinery of these viruses to deliver Cas9 and guide RNA cargo into cells that resist conventional transfection &mdash; including primary cells, stem cells, neurons, and in vivo tissues. AAV is the most widely used viral vector for CRISPR-based gene editing, valued for high transduction efficiency, low immunogenicity, and an established safety profile in human gene therapy. The right viral delivery system influences transduction efficiency, expression profile, and genome-editing outcomes. AAV and adenoviral vectors offer distinct advantages depending on your research goals.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li data-section-id="17k5oh4" data-start="0" data-end="142">Adenoviral Cas9 &amp; Nickase Nucleases: Viral delivery systems for rapid, transient Cas9 or nickase expression without genomic integration.</li>
<li data-section-id="12kolej" data-start="144" data-end="280">AAV6 &amp; AAV8 Viral Vectors: Serotype-specific AAV vectors optimized for transduction of hematopoietic stem cells and liver tissues.</li>
<li data-section-id="81toc4" data-start="282" data-end="412">saCas9 AAV Serotypes (1&ndash;10): Diverse AAV serotypes designed to support targeted cellular tropism across CRISPR applications.</li>
<li data-section-id="18h5j1a" data-start="414" data-end="554">Ampicillin-Selected AAV Vectors: AAV vector backbones containing ampicillin resistance markers for cloning and transformant selection.</li>
<li data-section-id="xfx9g1" data-start="556" data-end="697" data-is-last-node="">High-Titer Viral Particles: Purified viral preparations optimized to maintain genomic integrity and minimize endotoxin-related effects.</li>
</ul>
<h2 dir="ltr"> How to Choose AAV/Adenovirus CRISPR Delivery</h2>
 Genome Capacity Requirements AAV's ~4.7 kb packaging capacity is too small for full-length SpCas9 (~4.2 kb) plus a promoter and guide RNA. Split-vector systems (Cas9 across two AAV particles) or compact Cas9 orthologs such as SaCas9 (~3.2 kb) address this limit. Adenovirus offers 34&ndash;43 kb capacity for larger cargo. Serotype and Tissue Tropism AAV serotypes differ in their ability to transduce specific cell types and tissues, making serotype selection an important part of CRISPR delivery design. The optimal serotype depends on the target tissue, model system, administration route, and experimental goals. MBP offers saCas9 AAV viruses in multiple serotypes (AAV1&ndash;AAV11) to support a range of research applications. Cell Division Status Both AAV and adenovirus transduce dividing and non-dividing cells, making them suitable for primary neurons, hepatocytes, and other post-mitotic populations that resist standard transfection. Immunogenicity Considerations AAV has low immunogenicity and minimal integration risk, supporting widespread pre-clinical and clinical in vivo use. Adenovirus offers higher initial transduction efficiency and larger cargo capacity but generates a stronger immune response, which can limit in vivo utility. Packaging System Helper-free packaging systems eliminate the need for replication-competent helper virus, designed for BSL-2 containment &mdash; though institutional biosafety committee (IBC) approval is required before starting viral vector work. 
<h2 dir="ltr"> Specifications Context</h2>
 AAV and adenoviral CRISPR delivery systems support retinal gene therapy models, CNS editing studies, liver-targeted in vivo CRISPR, muscle cell engineering, primary immune cell editing, iPSC-derived cell modification, and genome-modified animal model generation. Capsid engineering through directed-evolution platforms continues to expand AAV tropism options, including enhanced CNS penetration following systemic delivery, an active area of pre-clinical research relevant to labs planning long-term in vivo programs. All recombinant AAV production and adenoviral vector work requires IBC approval at BSL-2 containment in most jurisdictions; MBP can provide biosafety classification documentation. Product availability reflects MBP's catalogue as of mid-2026. 
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Reach out to the professional <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">team at MBP Inc</a>. now to ask for a quote for a suitable AAV or Adenovirus system for your lab.