Brands

<h2 data-section-id="1go10h8" data-start="173" data-end="215">What are classic cell culture media?</h2>
&nbsp;
Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture since the mid-20th century: DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's Nutrient Mixtures F-10 and F-12, IMDM (Iscove's Modified Dulbecco's Medium), and the combination formulation DMEM/F-12. Each is manufactured according to its originally published formula, with modifications limited to those necessary for consistent performance and stability of the medium over its shelf life. These media supply the amino acids, vitamins, glucose, salts, and (in most formulations) phenol red pH indicator that form the nutritional backbone of essentially all subsequent specialty, serum-free, and Advanced media derivatives.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Selective Mammalian Antibiotics including Puromycin, Geneticin, and Hygromycin B for stable development of cell lines.</li>
<li dir="ltr" role="presentation">Broad-Spectrum Bactericidal Agents for routine contamination prevention, including Penicillin/Streptomycin and Gentamicin&nbsp;</li>
<li dir="ltr" role="presentation">Prokaryotic Selection Reagents for effective bacterial transformation, including Ampicillin, Kanamycin, Chloramphenicol, and Tetracycline&nbsp;</li>
<li dir="ltr" role="presentation">Antifungal and Antimycotic Solutions to maintain sterile cultures, including combined 100X cocktails and Amphotericin B (Fungizone)&nbsp;</li>
<li dir="ltr" role="presentation">Specialized Small Molecules for targeted biochemical assays, including Ganciclovir, Rapamycin, and Actinomycin D&nbsp;</li>
</ul>
&nbsp;
<h2 data-section-id="1mfajxk" data-start="1218" data-end="1252">How to choose a classic medium</h2>
&nbsp;
DMEM vs MEM
DMEM contains up to four times the amino acid and vitamin concentration of original Eagle's MEM, along with higher glucose levels, making it suitable for cell types with elevated nutrient demands &mdash; DMEM is broadly applicable across mammalian, insect, and plant cell phenotypes including primary cells, stem cells, and transformed lines. MEM remains widely used for monolayer culture of many standard cell types, with optional Hanks' or Earle's salt formulations and a calcium-free modification that permits suspension growth.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640, developed at the Roswell Park Memorial Institute for culturing human leukemic cells, is optimized for non-adherent cell types &mdash; lymphocytes and other blood cells &mdash; and contains glutathione plus higher biotin, B12, and PABA concentrations than DMEM or MEM. Despite its hematopoietic origin, RPMI 1640 supports a broad range of adherent lines (HeLa, T47D, RENCA, CT26.WT) when properly supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were originally developed for CHO cell clones, HeLa cells, and mouse L-cells, and remain suited to hepatocyte culture, viral fusion experiments, and toxicity assays, with formulations available for use with or without serum. Coon's modification of Ham's F-12 roughly doubles the amino acid and pyruvate content and adds ascorbic acid, developed specifically for hybrid cells produced by viral fusion.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 (1:1 mixture) combines DMEM's glucose, amino acid, and vitamin richness with Ham's F-12's zinc, putrescine, hypoxanthine, and thymidine, producing an extremely rich complex medium used for MDCK cells, glial cells, fibroblasts, and human endothelial cells &mdash; and serving as the basal medium for many serum-free Advanced formulations used in organoid and stem cell work.
IMDM for high-density rapid proliferation
IMDM further enriches DMEM with additional amino acids and vitamins, substituting potassium nitrate for ferric nitrate and adding HEPES and sodium pyruvate. IMDM's sodium bicarbonate buffer system (5&ndash;10% CO₂) and enriched composition make it well suited for rapidly proliferating, high-density cultures including Jurkat, COS-7, and macrophage cells.
Liquid vs. powder format
Liquid classic media are ready to use (after appropriate FBS and supplement addition) and include sodium bicarbonate in the formulation. Powdered media are manufactured without sodium bicarbonate, since it tends to off-gas in powder form &mdash; 3.7 g/L sodium bicarbonate must be added upon reconstitution, followed by sterile filtration. Powder formats offer cost and shipping-weight advantages for high-volume labs preparing their own media.
&nbsp;
<h2 data-section-id="17i0pj" data-start="5035" data-end="5061">Specifications context</h2>
&nbsp;
Classic media are manufactured according to originally published formulas, with stability-related modifications documented per product. DMEM's sodium bicarbonate buffer system at 3.7 g/L is standard across liquid formulations; powdered formats require this addition upon reconstitution along with 0.22 &micro;m sterile filtration. As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the three most widely specified classic media across academic and biomanufacturing labs, with IMDM increasingly used for high-density suspension applications in bioprocessing.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents (GlutaMAX, NEAA, sodium pyruvate, HEPES), see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get highly efficient microbial control solutions for your lab.

Antibiotics

MSDS

Ampicillin

Unit Quantity

Concentration

Storage Condition

Shipping condition

Ampicillin Sodium

Zymo Research A1001-ZYM: Ampicillin Sodium solution, 100 mg/mL, ≥98% purity, sterile-filtered (0.2 µm). Working concentration 20–100 µg/mL. Store at –20°C. Ready-to-use for E. coli antibiotic selection. Made in USA. RUO.

Product Storage

Purity

Working Concentration

Manufacturer

Shipping Condition

Ampicillin Sodium (100 mg/ml) (5 x 5 ml)

Ampicillin Sodium (100 mg/ml) (5 ml)

<h2 dir="ltr">What are specimen collection swabs?</h2>
 Specimen collection swabs are sterile, single-use devices with a fiber tip on a plastic or wire shaft, used to acquire material from a patient's nasopharynx, anterior nares, oropharynx, wound, skin, or environment for diagnostic testing or microbial culture. Modern flocked swabs replace yards of wrapped fiber with perpendicular nylon or polyester fibers attached to the applicator, giving roughly 97 % specimen release on vortex versus about 33 % for traditional rayon or polyester. Buy from this category whenever specimen acquisition feeds a downstream culture or PCR &mdash; the swab determines whether the assay sees the analyte at all. 
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Sterile flocked swabs for high-efficiency sample collection and release</li>
<li dir="ltr" role="presentation">Polyester and rayon swabs for general microbiology sampling</li>
<li dir="ltr" role="presentation">Nylon flocked nasopharyngeal and/or oropharyngeal swabs</li>
<li dir="ltr" role="presentation">Dry swabs for direct plating or molecular testing workflows</li>
<li dir="ltr" role="presentation">Swabs with transport tubes for stabilized sample transfer</li>
<li dir="ltr" role="presentation">Breakpoint swabs for easy release into collection vials</li>
</ul>
<h2 dir="ltr"> How to choose specimen collection swabs</h2>
&nbsp;
Match the collection site Nasopharyngeal swabs use long flexible shafts (~150 mm) and small flocked or molded tips designed to reach the posterior nasopharynx. Anterior nares swabs are shorter and gentler. Oropharyngeal and wound swabs come in foam or flocked variants depending on the target organism.
Choose tip material for the assay Flocked nylon or polyester is the default for respiratory virus PCR. Cotton, calcium alginate, and wood-shafted swabs are excluded by CDC guidance because cotton contains PCR-inhibitory fatty acids and calcium alginate inhibits PCR directly. Foam suits some bacterial cultures and rapid antigen tests.
Verify the breakpoint and the shaft A predetermined breakpoint at a defined distance from the tip (commonly 100&ndash;101 mm for nasopharyngeal swabs) lets the operator snap the shaft into the transport tube; shafts should be plastic or wire, never wood.
Confirm sterility and FDA listing Single-use sterile peel pouches are the clinical standard. Swabs are FDA Class I exempt devices; verify the manufacturer's FDA registration and listing on the certificate of conformity.
<h2 dir="ltr"> Specifications context</h2>
 Diagnostic swabs are characterized by tip material, shaft material, total length, breakpoint distance, and sterility status. Nasopharyngeal flocked swabs commonly measure about 150 mm overall with a breakpoint near 100 mm and a flexible plastic shaft; the MedSchenker CavSwab uses Allium&trade; flock technology on a 153 mm shaft with a 101.3 mm breakpoint. Specimen release ranges from roughly 33 % for traditional rayon/polyester up to about 97 % for flocked nylon. As of 2026, these sit in a low-to-mid price tier per swab and are typically sold in case quantities of 100 or 500.&nbsp; 
&nbsp;
For swab selection, material compatibility, and sample collection formats,<a href="https://mbpinc.net/contact-us"> the MBP team&nbsp;</a>can guide you to the most suitable option based on your testing method and workflow setup.

Swabs

Anterior Nasal swab

AC4100 by MedSchenker is the CavSwab™ Anterior Nare swab, featuring a precision scalloped nylon flocked tip at 80 mm total length (no breakpoint) for enhanced anterior nostril specimen collection. E-beam sterile, medical-grade polypropylene shaft. Case of 5,000 units. Compatible with VTM, UTM, antigen testing, and molecular assays.

Package

Inner box

Length

Breakpoint

Shelf life

<h2>What is DNA/RNA Methylation as a reagent category?</h2>
&nbsp;
DNA/RNA Methylation covers the detection and quantification side of epigenetics: the reagents used to identify which cytosines in a DNA sample are methylated, distinguish 5-methylcytosine from 5-hydroxymethylcytosine, and detect RNA methylation marks like N6-methyladenosine (m6A). Methods range from bisulfite conversion, the historically standard approach for single-base-resolution cytosine methylation mapping, to enzymatic conversion as a less damaging alternative, to antibody-based immunoprecipitation for broad-scale enrichment of methylated DNA regions, to direct ELISA-based quantification of global methylation levels.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/methylation-standards">DNA methylation standards:</a> for accurate quantitative analysis and calibration of methylation levels
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/bisulfite-conversion-kits">Bisulfite conversion kits: </a>for efficient conversion of unmethylated cytosines in DNA methylation studies
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/bisulfite-conversion-reagents-and-buffers">Bisulfite Conversion Reagents and Buffers: </a>for enhanced processing of DNA and flexible workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/non-bisulfite-kits-and-reagents">Non-Bisulfite Kits and Reagents: </a>for alternative epigenetic profiling and methylation analysis methods
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/elisa-kits-and-reagents" style="color: #236fa1;">ELISA Kits and Reagents:</a> for quantifying sensitive DNA/RNA methylation levels
</li>
</ul>
&nbsp;
<h2>How to choose DNA/RNA methylation analysis reagents</h2>
&nbsp;
Choose bisulfite conversion for single-base-resolution cytosine methylation mapping
Bisulfite conversion converts unmethylated cytosines to uracil while leaving 5-methylcytosine largely unchanged under standard conditions, and after amplification and sequencing, the resulting base-pair difference reveals exactly which cytosines were methylated, making it the approach for single-base-resolution mapping but one that introduces significant DNA damage and degradation.
Consider enzymatic conversion as a less damaging alternative to bisulfite treatment
Enzymatic conversion methods achieve the same cytosine-to-uracil discrimination as bisulfite but through enzymatic rather than harsh chemical reactions, minimizing DNA damage and producing higher-quality libraries from fewer sequencing reads, at potentially higher reagent cost.
Use antibody-based MeDIP for broad methylation enrichment without locus-by-locus resolution
Methylated DNA immunoprecipitation uses an antibody against 5-methylcytosine or 5-hydroxymethylcytosine to enrich methylated DNA regions from a fragmented sample, providing genome-wide enrichment data without the single-base resolution of bisulfite sequencing.
Confirm your method can distinguish 5-mC from 5-hmC if that difference matters for your study
Standard bisulfite conversion cannot reliably distinguish 5-methylcytosine from 5-hydroxymethylcytosine, since both resist bisulfite-mediated deamination under standard conditions, so confirming whether your study requires that distinction helps determine whether a specialized additional step is needed.
Choose ELISA-based kits for rapid, global methylation quantification without sequencing
A 5-methylcytosine ELISA kit provides a rapid, quantitative readout of the global level of DNA methylation in a sample without requiring bisulfite conversion or sequencing, useful for broad-level comparisons across multiple conditions or samples when single-base resolution isn't needed.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis due to its single-base resolution, but it introduces significant technical challenges for amplification and next-generation sequencing library preparation due to DNA degradation, reduced genome complexity, and alignment complexity from the cytosine-to-uracil base conversion. As of 2026, enzymatic conversion is increasingly recommended over bisulfite conversion for methylation analysis specifically because it minimizes these DNA damage and quality issues, enabling improved preservation and detection of cytosine methylation marks from fewer sequencing reads with no change to downstream analysis pipelines.
<a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> today and get reliable DNA/RNA methylation kits for your lab.

DNA/RNA Methylation

Protocol

Anti-5-Hydroxymethylcytosine Polyclonal Antibody

A4001-ZYM by Zymo Research — Anti-5-Hydroxymethylcytosine Polyclonal Antibody, rabbit IgG, 1 mg/ml in PBS with 0.05% sodium azide. Validated for hmeDIP, ELISA, immunohistochemistry, and dot blot detection of 5-hmC in DNA from mammalian, plant, insect, and microbial sources. Available in 25, 50, and 200 µg sizes.

Buffer Solution

Isotype

Protein Concentration

Source

Specificity

Storage

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (200 µg)

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (25 µg)

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (50 µg)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl)

A3002-ZYM by Zymo Research — Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21), mouse IgG1, 0.5 µg/µl in PBS with 0.05% sodium azide. Validated for MeDIP, ELISA, and dot blot; specific for 5-mC in single-stranded DNA with no detectable cross-reactivity to 5-hmC or unmodified cytosine. Available in 15–200 µl sizes.

Clone

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (15 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (200 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (30 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (50 µl)

<h2>What are 5-hmC ELISA kits?</h2>
&nbsp;
5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
&nbsp;
<h2>How to use 5-hmC ELISA kits</h2>
&nbsp;
Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

Anti-DNA HRP Antibody (100X)

For use with Zymo Research ELISA products

Anti-DNA HRP Antibody (100X) (100 µl)

Anti-DNA HRP Antibody (100X) (200 µl)

Antibiotic/Antimycotic (100X)

G273 by ABM is a 100X Antibiotic/Antimycotic solution (100 mL) containing penicillin G (10,000 U/mL), streptomycin sulfate (10,000 µg/mL), and amphotericin B (25 µg/mL). Use at 1% v/v in mammalian cell culture medium. Store at −20°C.

Usage

Sterility

<h2 data-section-id="3d1466" data-start="0" data-end="47">What are cell culture buffers and solutions?</h2>
&nbsp;
Cell culture buffers and balanced salt solutions are isotonic solutions used to wash, dilute, transport, and temporarily maintain cells outside of complete growth media. Unlike culture media, these solutions do not contain the nutrients and growth factors required for cell proliferation. Instead, their primary role is to maintain physiological pH and osmotic balance while protecting cells from stress during handling and experimental procedures.
The most commonly used cell culture buffers include PBS (Phosphate-Buffered Saline), DPBS (Dulbecco's Phosphate-Buffered Saline), HBSS (Hank's Balanced Salt Solution), and EBSS (Earle's Balanced Salt Solution). These solutions are widely used in cell washing, passaging, tissue dissociation, sample preparation, immunoassays, and short-term cell maintenance.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Standard Isotonic Buffers to wash cells, maintain osmotic balance, and dilute reagents.</li>
<li dir="ltr" role="presentation">Biological Buffering Agents for metabolic support and pH maintenance.</li>
<li dir="ltr" role="presentation">Cell Dissociation &amp; Neutralizing Reagents maintain high cell viability and are used for cell recovery, detachment, and passaging</li>
<li dir="ltr" role="presentation">Surface Coating &amp; Matrix Solutions with high-grade gelatin for improved cell attachment.</li>
<li dir="ltr" role="presentation">Insulin-Transferrin-Selenite (ITS), Bovine Serum Albumin (BSA), and high-purity Holo-Transferrin for enhanced cell growth and viability</li>
</ul>
&nbsp;
<h2 data-section-id="1cuz7jf" data-start="1165" data-end="1216">How to choose cell culture buffers and solutions</h2>
&nbsp;
PBS vs. DPBS PBS is a simple sodium chloride/phosphate buffer solution. DPBS adds potassium chloride and may include calcium, magnesium, glucose, and pyruvate depending on formulation. The practical difference between PBS and DPBS formulations is minimal &mdash; both maintain pH 7.2-7.4 and osmolality ~280-315 mOsm/kg and are functionally interchangeable for standard washing, immunoassay, and sample preparation applications. If your protocol is sensitive to minor salt concentration differences, compare specific formulation sheets rather than assuming equivalence. &nbsp; Calcium/magnesium-free vs. calcium/magnesium-containing Calcium and magnesium facilitate cell-cell and cell-surface adhesion. Calcium/magnesium-free PBS, DPBS, or HBSS is required: before enzymatic dissociation (residual Ca/Mg inhibits trypsin, requiring more enzyme and harsher conditions &mdash; the most common cause of poor trypsinization results), for rinsing EDTA or other chelators from culture, and for minimizing cell-cell aggregation during gentle dissociation or suspension recovery. Calcium/magnesium-containing formulations support cell binding and clumping, useful for transport applications or attachment-dependent procedures. &nbsp; HBSS/EBSS vs. PBS/DPBS for short-term maintenance HBSS and EBSS include sodium bicarbonate buffering and may include glucose, providing meaningfully better short-term physiological support for cells &mdash; particularly under CO₂ atmosphere &mdash; than the simpler PBS/DPBS formulations. Use HBSS/EBSS when cells need to remain viable for a meaningful period outside complete growth media (tissue dissociation workflows, transport, short-term holding). Use PBS/DPBS for washing steps, immunoassay/Western blot dilutions, and sample preparation where osmotic balance &mdash; not metabolic support &mdash; is the requirement. &nbsp; Phenol red and glucose/pyruvate variants DPBS and HBSS are available with or without phenol red and with or without glucose/pyruvate, allowing matching to downstream applications (phenol red-free for fluorescence work; glucose/pyruvate-containing for short-term metabolic support). &nbsp; Endotoxin and quality testing Regular quality testing of balanced salt solutions includes osmolality, pH, stability, and screening for endotoxins and bacterial/fungal contamination. For immune cell handling and other endotoxin-sensitive applications, premium formulations document endotoxin levels below 0.005 EU/mL &mdash; a meaningfully tighter specification than standard research-grade buffers. &nbsp; Powder vs. liquid format Powdered balanced salt solutions are manufactured without sodium (to avoid sodium bicarbonate off-gassing during storage), requiring sodium bicarbonate addition, pH adjustment, and sterile filtration at time of preparation &mdash; following the same principle as powdered classic media.Specifications context
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Most balanced salt solutions are formulated to maintain physiological conditions with a pH of approximately 7.2&ndash;7.4 and osmolality ranging from about 280&ndash;315 mOsm/kg. These properties help minimize osmotic stress and maintain cell viability during handling.
Calcium- and magnesium-free DPBS remains one of the most commonly specified buffers for cell dissociation workflows because it improves the effectiveness of trypsin and other detachment enzymes. HBSS and EBSS continue to be widely used when short-term metabolic support is required outside complete growth media.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-purity buffers and solutions for your lab.

Buffers & Solutions

Buffers/Salts

Apo-Transferrin

Appearance

Disclaimer

G422-lot. E05091642E CoA

Applied Cell Extracellular Matrix

G422 by ABM — Applied Cell Extracellular Matrix, 25 mL liquid protein coating for cell adhesion, growth, differentiation, and migration. Apply as thin film (75 µL per 96-well to 15–17 mL per T175 flask) or solid gel. Store at 4–8°C, do not freeze, 1-year shelf life. Lot-tested negative for bacterial and fungal contamination.

Laboratory Aspirator

<h2>What Are Laboratory Aspirators?</h2>
&nbsp;
Laboratory aspirators are compact vacuum systems designed to remove liquids from cell culture vessels, microplates, centrifuge tubes, and other laboratory containers without manual pipetting. These systems typically consist of an oil-free vacuum pump, adjustable pressure controls, a collection bottle, overflow protection mechanisms, replaceable filters, tubing, and handheld aspiration tools. Laboratory aspirators are widely used for cell culture media removal, supernatant aspiration, and washing procedures in applications such as ELISA, immunoassays, and general laboratory workflows.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li><a href="https://mbpinc.net/equipments/automated-liquid-handling/laboratory-aspirator"> Laboratory Aspirator: </a> Innovative systems like tabletop vacuum models and the Aspire&trade; model designed for routine waste handling, liquid removal, and cleanup of benchtop samples.</li>
<li><a href="https://mbpinc.net/equipments/automated-liquid-handling/laboratory-aspirator/laboratory-aspirator-accessories"> Laboratory Aspirator Accessories: </a> Adapters (single and multi-channel), silicone tubing, hydrophobic filters, handheld controllers, and collection bottles for suction setup optimization.</li>
</ul>
&nbsp;
<h2>How to Choose a Laboratory Aspirator</h2>
&nbsp;
Vacuum Pressure Range and Adjustability Choose a system with adjustable vacuum settings that match your applications. Lower vacuum levels are suitable for delicate cell culture work, while higher settings support rapid liquid removal from tubes, flasks, and larger vessels.
Collection Bottle Capacity Collection bottle size influences how frequently waste must be emptied during operation. Standard capacities are suitable for most workflows, while larger bottles support high-throughput or extended use.
Overflow Protection Features Reliable overflow protection prevents liquid from reaching the vacuum pump and ensures safe handling of biological materials.
Handpiece and Tip Compatibility Ensure the handpiece supports pipette tips, Pasteur pipettes, and required adapters for your workflow.
Noise Levels and Biosafety Cabinet Compatibility Compact, quiet systems are suitable for use in or near biosafety cabinets without disrupting airflow or workspace access.
Autoclavable Components Check which parts are autoclavable to simplify sterilization and maintain laboratory hygiene standards.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Modern laboratory aspirators typically include oil-free pumps, adjustable vacuum controls, overflow protection systems, and replaceable hydrophobic filters for safe and efficient liquid removal.
Proper maintenance, including filter replacement and tubing inspection, helps maintain performance and extends equipment lifespan. Laboratory aspirators remain essential tools in research, clinical, and biotechnology laboratories.
Your hunt for a laboratory aspirator is over. <a href="https://mbpinc.net/contact-us">Reach out to the expert team</a> at Molecular Biology Products Inc. (MBP) today for a personalized quote.

Product Literature

User Manual

Aspire™ Laboratory Aspirator

The V0020 by Benchmark Scientific (Aspire Laboratory Aspirator) is a self-contained vacuum aspiration system for cell culture and liquid waste removal. Internal maintenance-free pump; 14 mL/sec max aspiration, 15 L/min flow, 0 to -750 mbar fully adjustable vacuum. Includes: 2 L graduated polycarbonate collection bottle with overflow protection, replaceable hydrophobic filter, ergonomic handheld vacuum pipette with two accessory cones (200-300 uL tip cone + stainless capillary). 115V US plug. Replacement parts: V0020-SG (O-ring), V0020-F1 (filter), V0020-TB (tubing).

Max. Aspiration

Max. Flow

Vacuum Range

Noise Level

Vacuum Pump

Dimensions

Weight

Electrical

Warranty

Manufacture

Aspire, Laboratory Aspirator with single channel flow controller, 115V

Aspire, Laboratory Aspirator with single channel flow controller, 230V

<h2>What are epigenetics enzymes and inhibitors?</h2>
&nbsp;
Epigenetics enzymes and inhibitors are the molecular tools for directly studying or modulating the enzymatic machinery that writes, erases, or reads epigenetic marks. The major classes include: DNA methyltransferases (DNMT1, DNMT3A, DNMT3B), which catalyze the addition of a methyl group to cytosine; TET family enzymes (TET1, TET2, TET3), which oxidize 5-methylcytosine to 5-hydroxymethylcytosine; histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups from histone lysine residues; and histone methyltransferases (HMTs) including EZH2, DOT1L, and G9a. Small-molecule inhibitors of HDAC and DNMT enzymes are the most clinically and experimentally advanced of these tools.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Micrococcal nuclease for nucleosome mapping and controlled chromatin digestion applications</li>
<li dir="ltr" role="presentation">DNA shearing enzymes for consistent fragmentation of DNA in sequencing and epigenetic workflows</li>
<li dir="ltr" role="presentation">DNA degradation enzymes for controlled breakdown and analysis of nucleic acids&nbsp;</li>
<li dir="ltr" role="presentation">CpG methylase enzymes for targeted DNA methylation (CpG-specific )&nbsp; in epigenetic modification studies</li>
<li dir="ltr" role="presentation">5-hmC glucosyltransferase for detection and labeling of hydroxymethylcytosine in DNA</li>
<li dir="ltr" role="presentation">SAM (S-adenosylmethionine) cofactors for supporting enzyme activity and methylation reactions</li>
<li dir="ltr" role="presentation">DNA digestion enzymes for genomic DNA processing workflows</li>
<li dir="ltr" role="presentation">dsDNA nuclease systems for controlled cleavage and analysis of double-stranded DNA</li>
</ul>
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<h2>How to choose epigenetics enzymes and inhibitors</h2>
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Choose DNMT inhibitors to globally reduce or deplete DNA methylation in cells
Nucleoside analog DNMT inhibitors like 5-azacytidine and decitabine incorporate into DNA and trap DNMT1, preventing maintenance methylation and causing passive demethylation through DNA replication, while newer non-nucleoside DNMT inhibitors are in development for more selective, non-genotoxic methylation reduction.
Choose HDAC inhibitors to globally increase histone acetylation
HDAC inhibitors including pan-HDAC hydroxamates like vorinostat (SAHA) or class-selective compounds reduce histone deacetylase activity and cause hyperacetylation of histone lysine residues, broadly promoting gene expression at silenced loci. HDAC inhibitors are the most clinically advanced class of epigenetic therapeutics, with multiple FDA-approved compounds.
Use TET enzyme preparations for in vitro 5-hmC oxidation studies
Purified TET1, TET2, or TET3 enzyme preparations allow controlled in vitro oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products, useful for generating defined 5-hmC-containing substrate DNA or studying TET enzyme activity and selectivity.
Choose EZH2 or other HMT inhibitors for studying PRC2-dependent gene silencing
EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), catalyzes H3K27 trimethylation (H3K27me3), a histone modification associated with repression of tumor suppressor genes, and EZH2 inhibitors like tazemetostat are used in research to probe the role of this silencing mark.
Combine inhibitors to study epistatic relationships between epigenetic marks
Because DNA methylation, histone acetylation, and histone methylation are mechanistically linked, combining HDAC inhibitors with DNMT inhibitors can produce synergistic effects on gene expression exceeding either alone, and these combination approaches are both experimental tools and the basis for combination epigenetic therapy research.
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<h2>Specifications context</h2>
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HDAC and DNMT inhibitors are the most clinically advanced classes of epigenetic therapeutics, with multiple HDAC inhibitors including vorinostat and romidepsin approved for hematological malignancies, EZH2 inhibitors including tazemetostat having received accelerated approval, and multiple BET inhibitors in clinical trials. As of 2026, CRISPR/dCas9 systems fused with DNMTs, TETs, and HDACs enable locus-specific epigenome editing, extending classic enzyme and inhibitor approaches toward targeted epigenetic modulation.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and find the suitable enzymes and inhibitory reagents for your lab.

Enzymes & Inhibitors

Atlantis Digestion Buffer (1X) (50 ml)

Atlantis Digestion Buffer (1X), SKU E2030-1 by Zymo Research — 50 mL ready-to-use companion buffer for the Atlantis dsDNase (E2030) enzyme. Used for nucleosomal DNA preparation in ChIP, ATAC-seq, and nucleosome positioning workflows. Store at −20°C; part of the EZ Nucleosomal DNA Prep Kit (D5220).

Atlantis dsDNAse (12.5 U)

E2030 by Zymo Research is Atlantis dsDNAse, 12.5&nbsp;U at 0.1&nbsp;U/&micro;l (125&nbsp;&micro;l). Selectively digests dsDNA without degrading ssDNA or RNA. Used for nucleosome mapping and ChIP-seq chromatin fragmentation. Storage: &minus;20&deg;C.