Brands

High-Fidelity Master Mix

<h2 dir="ltr">What Is a High-Fidelity PCR Master Mix?</h2>
 A high-fidelity PCR master mix is a 2x concentrated, pre-formulated solution containing a proofreading DNA polymerase (Phusion, Q5, KOD, or Pfu-equivalent), dNTPs, MgCl2, and reaction buffer &mdash; requiring only the addition of template and primers to set up a reaction. The proofreading polymerase's 3' to 5' exonuclease activity corrects misincorporated nucleotides during synthesis, reducing the error rate roughly 50-100-fold compared to standard Taq master mixes. Choose a high-fidelity master mix whenever the amplicon will be cloned, mutagenised, or sequenced &mdash; not for routine genotyping, where standard Taq is sufficient and lower cost.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="uyjmeg" data-start="418" data-end="539">Streamlined post-amplification analysis with high-fidelity 2X red mix formulations with direct gel-loading capability</li>
<li data-section-id="1rh0fvx" data-start="540" data-end="630">Pre-optimized high-fidelity 2X PCR master mixes containing proofreading DNA polymerase</li>
<li data-section-id="6drhog" data-start="631" data-end="735">Reduced non-specific amplification and primer-dimer formation compared to standard Taq-based systems</li>
<li data-section-id="1pa7u4r" data-start="736" data-end="826">High-fidelity amplification suitable for cloning workflows requiring sequence accuracy</li>
<li data-section-id="1n3ow6j" data-start="827" data-end="925">2X master mix formats designed for reproducible amplification across a range of template types</li>
</ul>
<h2 dir="ltr"> How to Choose a High-Fidelity Master Mix</h2>
 Downstream Application Cloning into expression vectors, site-directed mutagenesis, and sequencing-bound amplicons all benefit from the reduced error rate of a high-fidelity master mix. Routine genotyping or colony screening does not require this level of accuracy and is typically served by standard Taq master mixes at a lower cost. Template Difficulty (GC Content, Length) Some high-fidelity master mix formulations are specifically optimised for GC-rich templates or long amplicons (&gt;5 kb), where standard high-fidelity formulations may show reduced yield. Check product-specific template compatibility notes before ordering for difficult targets. Extension Time and Cycling Protocol High-fidelity polymerases often have different optimal extension times per kilobase compared to standard Taq &mdash; some extend faster, shortening overall cycling time for long amplicons. Follow the manufacturer's recommended cycling parameters rather than reusing a standard Taq protocol. Cloning Method Compatibility Some high-fidelity master mixes produce blunt-ended products suited to Gibson Assembly or blunt cloning strategies; others may need additional A-tailing for TA cloning. Confirm compatibility with your intended downstream cloning method before selecting a product. Format Convenience vs. Component Flexibility The 2x master mix format minimises pipetting steps and improves reproducibility for routine high-fidelity workflows. Researchers needing to titrate individual components (e.g., additional MgCl2 for a specific template) may prefer the individual high-fidelity enzyme plus separate buffer instead. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x high-fidelity master mix is set up identically to a standard 2x Taq master mix &mdash; combined with template and primers at the manufacturer-specified ratio to reach 1x working concentration &mdash; but the included proofreading polymerase detects and removes misincorporated bases immediately after insertion, reducing the per-cycle error rate to approximately 10⁻⁶ per nucleotide. This level of accuracy matters specifically because any error introduced during amplification is propagated into the final cloned construct or sequencing read. High-fidelity master mixes are typically stored at &minus;20 &deg;C; lot-specific CoAs documenting activity and quality control are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get pre-optimized High-Fidelity Master Mix solutions for your lab.

Accuris High Fidelity, Hot Start Master Mix

Accuris High Fidelity DNA Polymerase & Master Mix

Accuris High Fidelity PCR Master Mix

Accuris High Fidelity PCR Master Mix (PR1001-HF) by MBP delivers up to 100&times; higher fidelity than Taq in a ready-to-use 2X format. Proofreading activity, crude DNA compatible, blunt or A-overhang products. Optimized buffers with PCR enhancers. Sample, 200-, and 500-reaction packs. Store at &minus;20&deg;C.

Manufacturer

Accuris High Fidelity PCR Master Mix, sample, 5 rxns

Accuris High Fidelity Master Mix, 200 rxns

Accuris High Fidelity Master Mix, 500 rxns

Hot Start Taq Master Mix

<h2 dir="ltr">What Is a Hot Start Taq Master Mix?</h2>
 Hot start 2x master mixes combine the convenience of the ready-to-use master mix format with the improved specificity of hot-start technology. These products are the standard reagent format for high-throughput genotyping, clinical diagnostic PCR, and multiplex reactions where non-specific amplification would compromise results. The right hot-start master mix supports cleaner amplification and streamlined setup. Select a formulation designed for reliable PCR performance.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Hot-start formulations from Azura, Accuris, BlasTaq, and Zymo Research for reduced non-specific amplification and primer-dimer formation.</li>
<li>Antibody-mediated hot-start activation in selected products, including ZymoTaq PreMix and Accuris Hot Start Taq Master Mix.</li>
<li>2X master mix and premix formats containing hot-start Taq polymerase, MgCl₂, dNTPs, and optimized PCR buffers.</li>
<li>Red-dye formulations for direct gel loading, including Azura 2X HS Taq Red Mix and Accuris Hot Start Taq Master Mix Red Dye.</li>
<li>Buffer systems optimized for endpoint PCR, multiplex PCR, and challenging template amplification.</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a Hot Start Taq Master Mix</h2>
 Activation Mechanism Format Antibody-mediated hot-start master mixes activate during the standard initial denaturation step; chemically modified formats may require a longer or dedicated activation incubation &mdash; confirm cycling protocol compatibility before adopting a new product. Component Pre-Optimisation A 2x hot-start Taq master mix is a pre-formulated, double-concentrated solution containing hot-start Taq DNA polymerase, dNTPs, MgCl2, and PCR buffer &mdash; the hot-start polymerase remains inactive at room temperature until the activation step is reached during cycling. Multiplex Compatibility For multiplex PCR (multiple primer pairs in a single reaction), hot-start master mixes substantially reduce primer-primer cross-reactivity compared to standard Taq master mixes, improving reliable detection of all intended targets. Diagnostic and Clinical Applications High-specificity master mixes are the standard reagent format for clinical diagnostic PCR, where false-positive bands from non-specific amplification could affect result interpretation. Throughput and Format 2x hot-start master mixes are designed for high-throughput genotyping pipelines, requiring only template and primer addition &mdash; minimising pipetting steps across large sample sets. 
<h2 dir="ltr"> Specifications Context</h2>
 Hot-start 2x master mixes are set up like standard master mixes&mdash;combined with template and primers at the manufacturer-specified ratio to achieve a 1x working concentration&mdash;but the polymerase remains inactive until the activation step, typically during initial denaturation for antibody-mediated formats. This combination of convenience and specificity has made hot-start master mixes a widely used choice for high-throughput genotyping, multiplex PCR, and other applications requiring reliable target-specific amplification. Product availability reflects MBP's catalogue as of mid-2026. Labs validating a new hot-start master mix should evaluate performance using known positive and negative samples and document results by lot number to support quality assurance, audits, and troubleshooting. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and get premium-quality Hot Start Taq Master Mix formulations for your lab today.

Accuris Hot Start Taq DNA Polymerase & Master Mix

Accuris Hot Start Taq Master Mix Red Dye

Accuris Hot Start Taq Master Mix Red Dye (PR1001-HSR) by MBP combines antibody-mediated hot start PCR with a red loading dye for direct gel loading — no additional loading buffer needed. Ideal for low copy, multiplex, and GC-rich PCR with streamlined gel analysis. Sample, 200-, and 1,000-reaction packs. Store at &minus;20&deg;C.

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., sample, 5 reactions

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 200 reactions

Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 1000 reactions

Accuris Hot Start Taq Mastermix

Accuris Hot Start Taq Mastermix (PR1001-HS) by MBP is a ready-to-use 2X PCR master mix with antibody-mediated hot start, allowing room-temperature reaction setup with no non-specific amplification. Exceptional sensitivity for low copy, multiplex, and GC-rich PCR. Sample, 200-, and 1,000-reaction packs. Store at &minus;20&deg;C.

Accuris Hot Start Taq Mastermix, sample, 5 reactions

Accuris Hot Start Taq Master Mix, 2X conc., 200 reactions

Accuris Hot Start Taq Master Mix, 2X conc., 1000 reactions

Hot Start Taq DNA Polymerase

<h2 dir="ltr">What Is Hot Start Taq DNA Polymerase?</h2>
 Hot-start Taq DNA polymerase is the enzyme of choice for PCR applications where non-specific amplification or primer dimer formation has been problematic with standard Taq. By keeping the polymerase inactive until a high-temperature activation step, hot-start formulations prevent the room-temperature primer extension that drives non-specific products in standard Taq reactions. Choose a polymerase suited to your assay requirements.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1hsv5i3" data-start="233" data-end="391">Hot-start Taq DNA polymerases are designed to support multiplex PCR by reducing non-specific amplification and primer-primer interactions during reaction setup.</li>
<li data-section-id="bhgh5r" data-start="392" data-end="516">Formulations suitable for high-throughput PCR workflows requiring a stable room-temperature setup before thermal activation.</li>
<li data-section-id="1h2z623" data-start="517" data-end="640">Improved specificity for amplification of low-abundance targets compared with conventional non-hot-start Taq polymerases.</li>
<li data-section-id="1nifv8r" data-start="641" data-end="743">Cleaner amplicon generation for downstream applications such as cloning, sequencing, and genotyping.</li>
<li data-section-id="bof93a" data-start="744" data-end="892">Enzyme and buffer systems formulated to support amplification of challenging templates, including GC-rich regions and complex genomic DNA samples.</li>
</ul>
<h2 dir="ltr"> How to Choose Hot Start Taq DNA Polymerase</h2>
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Inactivation Mechanism Antibody-mediated formulations use a heat-sensitive antibody that reversibly binds and inhibits the enzyme until denatured during the initial high-temperature step. Chemically modified formulations use covalent modifications to active-site residues that are reversed by heat during cycling. Standard vs. Hot-Start Comparison Standard Taq DNA polymerase is a formulation of recombinant Taq that remains active at ambient temperatures, permitting non-specific primer extension during reaction setup &mdash; hot-start formulations are reversibly inactivated at ambient temperature specifically to prevent this. Buffer Compatibility As with standard Taq, optimised 10x PCR buffer formulations are designed to complement the specific hot-start enzyme's activation and activity profile. Activation Time Considerations Confirm whether your chosen hot-start formulation activates within a standard initial denaturation step or requires an extended/dedicated activation incubation, as this affects total cycling protocol time. Application Fit Hot-start Taq is recommended wherever specificity is a concern: multiplex PCR, low-template-abundance amplification, diagnostic PCR, and any workflow where primer dimer formation has been observed with standard Taq. 
<h2 dir="ltr"> Specifications Context</h2>
 Hot-start Taq DNA polymerase is a recombinant Taq formulation that remains inactive during reaction setup, preventing the non-specific primer extension that standard Taq can permit before thermal cycling begins. This improved specificity is particularly valuable for multiplex PCR, low-abundance templates, and applications where non-specific products could affect result interpretation. Hot-start enzymes are typically stored at &minus;20 &deg;C and commonly supplied with matched PCR buffer. Because antibody-mediated and chemically modified formats can require different activation conditions, initial denaturation parameters should be verified when introducing a new enzyme. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to request a quote and identify the most suitable hot start Taq DNA polymerase for your workflow.

Accuris Hot Start Taq Polymerase

Accuris Hot Start Taq Polymerase (PR1000-HS) by MBP is an antibody-mediated Hot Start enzyme for room-temperature reaction setup with high specificity. Ideal for multiplex PCR, GC-rich DNA, and low copy number targets. Available in sample, 500-, 1,000-, and 6,000-unit packs (PR1000-HS-S / PR1000-HS-500 / PR1000-HS-1000 / PR1000-HS-6000). Store at &minus;20&deg;C.

Accuris Hot Start Taq, sample, 50 units

Accuris Hot Start Taq, 500 units

Accuris Hot Start Taq, 1000 units

Accuris Hot Start Taq, 6000 units

Automated Purification Systems

<h2>What Are Automated Purification Systems?</h2>
&nbsp;
Automated purification systems are integrated chromatography instruments that combine a multi-channel solvent delivery pump, a sample injection module, a chromatography column holder (accepting prepacked cartridges or reusable columns), a UV/Vis (and optionally ELSD or mass detector) detection system, and an automated fraction collector into a single controlled platform. The system runs gradient elution programs that change solvent composition over time, separating sample components by their differential affinity for the stationary phase. UV signal peaks trigger fraction collection into numbered tubes or microplates, eliminating the need for the operator to monitor the separation and manually collect fractions. Mass-directed systems add an inline mass spectrometer to trigger fraction collection on a specific molecular mass rather than UV absorbance, enabling selective collection of the target compound even when it co-elutes with impurities of similar UV absorption. Specify an automated purification system when your lab runs more than 3 to 5 flash chromatography purifications per day; the time saved on each run compounds rapidly and improves fraction quality through consistent, reproducible gradient programming.
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<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<ul>
<li data-section-id="1xaq5zs" data-start="25" data-end="248">Compact and high-throughput magnetic bead-based instruments, including IsoPure&trade; Mini, IsoPure&trade; 24, IsoPure&trade; 96, and Nucleic Acid Purifier 16 systems for automated nucleic acid purification.</li>
<li data-section-id="124t8zs" data-start="249" data-end="383">Compatible 24-well and 96-well deep-well plates and elution plates for automated purification workflows.</li>
<li data-section-id="ucx4qa" data-start="384" data-end="516">Replacement magnetic tip combs for IsoPure&trade; Mini, IsoPure&trade; 24, and IsoPure&trade; 96 automated purification systems.</li>
<li data-section-id="15pcqjt" data-start="517" data-end="657">Reagent vessels and aluminum sample racks designed for efficient sample loading and workflow organization.</li>
<li data-section-id="4rzy3m" data-start="658" data-end="817" data-is-last-node=""><a href="https://mbpinc.net/equipments/purification-and-separation-equipment/automated-purification-systems/automated-purification-system-accessories">System Accessories</a>, including Tablets, demo cases, and other compatible accessories to support the setup, operation, and maintenance of automated purification systems.</li>
</ul>
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<h2>How to Choose an Automated Purification System</h2>
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Flash chromatography vs. preparative HPLC
Automated flash chromatography systems (operating at 5 to 30 bar with spherical 25 to 100 micron silica or C18 cartridges) are optimized for fast, cost-effective purification of 0.1 to 10 g samples with throughputs of 3 to 10 runs per day. Preparative HPLC systems (operating at 50 to 300 bar with 5 to 25 micron particle columns) provide higher resolution for complex mixtures, smaller sample loads (mg to g scale), and are required when flash chromatography cannot resolve closely similar compounds. Some systems integrate both flash and prep HPLC capability in one instrument.
Sample load and column capacity
Match the column loading to your typical sample mass. Standard flash cartridges in 4 g, 12 g, 25 g, 40 g, 80 g, and 120 g silica sizes handle sample loads of roughly 0.04 to 1.2 g on normal-phase silica. Reversed-phase C18 cartridges handle lower loads per gram of silica. For larger sample loads (5 to 100 g), large-diameter columns or multi-injection protocols are used. Confirm the system's maximum column size and flow rate capacity against your typical sample mass.
Detection: UV/Vis vs. ELSD vs. mass directed
UV/Vis detection (monitoring at 254 nm and 280 nm) is standard and sufficient for compounds with a chromophore (most synthetic products). Evaporative light scattering detection (ELSD) is added for compounds with poor UV absorption (lipids, carbohydrates, alkyl chains). Mass-directed purification adds an inline single-quadrupole mass spectrometer that triggers fraction collection only when the target mass is detected; this is the highest-selectivity option for purifying one compound from a complex mixture, but at significantly higher instrument cost.
Number of solvent lines
Entry-level systems use two solvent lines (binary gradient). Advanced systems provide four solvent lines (quaternary gradient), enabling more complex separation strategies, modifier additions (e.g., acetic acid or ammonium formate in reversed-phase), and reduced operator setup time for multi-method labs. For most flash chromatography in synthetic chemistry, a binary gradient (organic/non-polar + polar modifier) is sufficient.
Software and workflow integration
Automated systems vary widely in software capability: basic systems provide gradient programming and peak detection; advanced platforms add automatic injection, queue management for multiple samples, solvent level monitoring, LIMS connectivity, and 21 CFR Part 11 compliance for regulated pharmaceutical environments. For open-access use in multi-chemist departments, queue-based software allowing each user to submit samples independently increases throughput.
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<h2>Specifications Context</h2>
&nbsp;
Column and cartridge consumables are a significant ongoing cost for automated purification systems; prepacked silica cartridges for flash systems are single-use or limited-use. Confirm cartridge availability and pricing for the specific system before purchase, as some systems lock users to proprietary cartridges at premium pricing while others accept standard-thread cartridges from multiple suppliers. Solvent consumption (L/run) scales with column size and flow rate; larger cartridges and longer gradients use more solvent. For high-throughput labs, solvent waste management (evaporation, recycling, or disposal) is a practical operational consideration. UV detection wavelength settings (254 nm for aromatic compounds, 210 to 220 nm for carbonyl-containing compounds) should be confirmed for your compound class to avoid missing low-UV-absorbing peaks.&nbsp;
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Complete your purification setup today&mdash;browse our accessories collection and <a href="https://mbpinc.net/contact-us">contact the MBP team</a> for a quote.

Accuris IsoPure Automated Purification Systems

User Manual

Typical Purification Process

IsoPure™ Mini, Automated Extraction Instrument

6-well sample strips for IsoPure Mini, pack of 100

8-place magnetic tip comb for IsoPure Mini, pack of 50

Android Tablet preloaded with IsoPure App

AP1024 by Accuris Instruments is the IsoPure™ 24, an automated magnetic bead-based nucleic acid purification system for up to 24 samples per run in deep-well plate format. The 8-position touchscreen-controlled carousel automates all purification steps — mixing, magnetic transfer, washing, and elution — in as little as 10 minutes. Compatible with KingFisher-format combs, 24-well deep-well plates, and all popular magnetic bead kits.

Manufectuer

IsoPure™ 24, Automated Purification System, 115V

IsoPure™ 24, Automated Purification System, 230V

One-Step RT-PCR Kits

<h2 dir="ltr">What Are One-Step RT-PCR Kits?</h2>
 A one-step RT-PCR kit combines reverse transcription and PCR amplification in a single reaction tube and buffer, using gene-specific primers throughout. The kit's enzyme mix typically pairs a reverse transcriptase -- commonly an M-MuLV-derived enzyme such as ProtoScript II or PrimeScript RT -- with a hot-start Taq DNA polymerase, sometimes including a proofreading component for higher-yield amplification.&nbsp; Choose a one-step kit for routine, single- or few-target RNA detection where simplicity and contamination control matter most; choose a two-step approach instead when one RNA sample needs to be tested against many different gene targets.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif; color: #000000;">One-step RT-PCR kits combining reverse transcription and PCR amplification in a single reaction workflow, including&nbsp;Azura 1-Step Ultra RT-PCR Kit</li>
<li style="color: #000000;">Standard one-step RT-PCR systems for RNA detection and target amplification, including One-Step RT-PCR Kit (RT0201-00) and Accuris One-Step RT-PCR Kit</li>
<li style="color: #000000;">Multiplex and probe-based one-step RT-qPCR-compatible systems, including Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit (No ROX)</li>
<li style="color: #000000;">High-performance one-step RT-PCR chemistry designed for robust RNA amplification, including the MegaFi One-Step RT-PCR Kit</li>
<li style="color: #000000;">Ready-to-use enzyme and buffer systems optimized for streamlined RT-PCR setup and reproducible results across RNA workflows</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-PCR Kit</h2>
 Enzyme Mix Composition Kits commonly combine an M-MuLV-derived reverse transcriptase with reduced RNase H activity and increased thermostability (e.g., ProtoScript II-type or PrimeScript-type enzymes) with a hot-start Taq polymerase. Some formulations add a proofreading polymerase component for higher-yield, more accurate end-point amplification. Hot-Start Activation Mechanism Most one-step kits use an antibody-modified or aptamer-based hot-start Taq that remains inactive at room temperature, dissociating during an initial high-temperature step (commonly around 94-95 &deg;C for several minutes) to simultaneously activate the polymerase and inactivate the reverse transcriptase before PCR cycling begins. Template Difficulty and Amplicon Length Reverse transcriptases with reduced RNase H activity and enhanced thermostability are better suited to GC-rich or highly structured RNA templates. Some one-step kits are validated for amplifying long transcripts -- up to approximately 9 kb in certain formulations -- relevant for full-length transcript or large-gene RT-PCR. Single-Tube Enzyme Compatibility Combining reverse transcriptase and Taq polymerase in one buffer historically risked mutual inhibition between the two enzyme classes; modern one-step formulations use optimised buffer systems and enzyme ratios specifically designed to avoid this interference, a key differentiator between basic and well-optimised kits. Detection Format End-point one-step RT-PCR kits (gel-based detection) suit qualitative presence/absence screening. For quantitative results, a one-step RT-qPCR kit with a fluorescent dye or probe system is the appropriate choice instead. 
<h2 dir="ltr"> Specifications Context</h2>
 The one-step format integrates the three RT-PCR steps -- reverse transcription, polymerase activation, and amplification -- into a single continuous reaction without opening the tube between steps, directly reducing contamination risk relative to workflows that transfer reaction products between vessels. Historically, combining reverse transcriptase and Taq polymerase in a shared buffer was reported to cause mutual enzyme inhibition and reduced sensitivity; current one-step kits address this through optimised buffer chemistry and enzyme selection, such as RNase H-minus reverse transcriptases paired with hot-start Taq formulations. One-step kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light, where fluorescent dyes are pre-included; lot-specific CoAs are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get high-precision One-Step RT-PCR Kits for your lab.

Accuris One-Step RT-PCR Kit

Accuris One-Step RT-PCR kit

Accuris One-Step RT-PCR Kit (PR1100) by MBP enables cDNA synthesis and PCR amplification in a single tube from as little as 1 pg total RNA. Includes thermostable reverse transcriptase and Hot-Start Taq. Available in sample, 50-reaction, and 100-reaction packs (PR1100-S / PR1100-50 / PR1100-100). Store at &minus;20&deg;C.

Accuris One-Step RT-PCR kit, sample 10 reactions

Accuris One-Step RT-PCR kit, 50 reactions

Accuris One-Step RT-PCR kit, 100 reactions

Cell Counter

<h2>What Are Automated Cell Counters?</h2>
&nbsp;
An automated cell counter is a benchtop image-cytometry instrument that captures a bright-field or fluorescence image of a cell suspension in a standardized counting chamber, then uses software algorithms to identify and count individual cells, distinguish live from dead cells based on staining, and calculate total cell density (cells/mL) and viability percentage. This replaces the manual hemocytometer method, in which a trained user counts cells under a compound microscope in a defined grid (a process that takes 5 to 15 minutes per sample and is subject to user interpretation differences of 10 to 20%). Image-based automated cell counters produce results in 5 to 10 seconds with inter-user CVs typically below 5%. They are used before every cell seeding step in cell culture workflows: before plating cells into microplates for assays, before seeding into bioreactors for suspension culture scale-up, before transfection or infection, before flow cytometry sample preparation, and during freeze-thaw recovery checks. Counting slide formats vary: most instruments use proprietary single-use counting slides (2-chamber slides loaded with 10 to 20 microliters per chamber); newer direct-pipette instruments load the sample directly onto an optical counting surface without a slide consumable. Automated cell counters replace the hemocytometer for routine cell density and viability counting; they are faster, less variable between users, and do not require a compound microscope.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Automated cell counters for rapid and accurate cell counting and viability analysis.</li>
<li><a href="https://mbpinc.net/equipments/microscopes-balances-and-analytical-equipment/microplate-instruments/cell-counter/cell-counter-accessories">Cell Counter Accessories</a>, including barcode scanners, standard test slides, and disposable QuadSlides for QuadCount cell counters.</li>
<li>Barcode scanners for streamlined sample identification and data management with QuadCount cell counters.</li>
<li>Standard test slides for instrument verification and performance checks.</li>
<li>Disposable QuadSlides for reliable cell counting with QuadCount automated cell counters.</li>
</ul>
&nbsp;
<h2>How to Choose an Automated Cell Counter</h2>
&nbsp;
Trypan blue vs. fluorescence viability staining
Trypan blue exclusion (mixing cells 1:1 with 0.4% trypan blue solution) is the standard viability method; dead cells with compromised membranes stain blue while live cells exclude the dye and appear bright. It is rapid, low-cost, and universally compatible with all automated cell counters. However, trypan blue cannot reliably distinguish dead cells from similarly sized debris, red blood cells, or cell aggregates. Dual fluorescence staining with acridine orange (AO, stains all nucleated cells green) and propidium iodide (PI, stains dead cells red) provides superior discrimination of live nucleated cells from dead cells and debris, and is required for accurately counting cells in complex suspensions (primary cells, heterogeneous populations, PBMCs) or cells with high baseline debris levels.
Counting range and cell size
Most automated cell counters specify a counting range of 5 x 10^4 to 1 x 10^7 cells/mL and a detectable cell diameter range of 4 to 60 micrometers. Confirm the instrument's range covers your typical culture density. For very small cells (bacteria at 1 to 3 micrometers) or large cells (cardiomyocytes above 30 micrometers), verify that the algorithm correctly sizes and counts the specific cell type. Many instruments include adjustable size and fluorescence threshold parameters to optimize counting for non-standard cell types.
Single-use slides vs. direct-pipette counting
Most automated cell counters use proprietary single-use disposable counting slides with two independent counting chambers, each loaded with 10 to 20 microliters of sample. Slides are the primary consumable cost driver at 0.50 to 2.00 USD per slide, depending on supplier and brand compatibility. Direct-pipette instrument positions the sample between two optical surfaces without a slide, eliminating the slide consumable cost and reducing per-sample cost to essentially zero consumable cost. The tradeoff is that direct-pipette systems require more thorough cleaning between samples to prevent carryover.
Throughput requirements
Single-sample automated cell counters (one sample per slide load) are adequate for most research labs, counting 5 to 20 samples per session. High-throughput variants accept cassettes of 8 or more slides and process them sequentially without user intervention per slide; appropriate for large cell culture facilities, counting dozens of bioreactor samples in sequence. Some instruments integrate with robotic liquid handlers for fully automated cell counting in high-throughput manufacturing environments.
GMP and regulated environment requirements
Cell counting in cell therapy manufacturing, vaccine production, and pharmaceutical bioprocessing requires validated cell counting methods with documented accuracy, precision, and linearity. Specify automated cell counters with IQ/OQ/PQ validation packages, 21 CFR Part 11-compliant data management software, and documented counting algorithm performance for the specific cell types being counted.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Cell counter accuracy is stated as a percentage deviation from a hemocytometer reference count for a defined cell type and density; validated instruments show less than 10% deviation across the counting range. Precision (intra-assay reproducibility) is expressed as CV% of repeat counts of the same sample; values below 5% are typical for automated counters on standard cell lines. Cell counters using bright-field imaging at a single focal plane may miscount cell clumps or overlapping cells; look for instruments with multi-focal-plane imaging or confocal-like focusing to improve accuracy on aggregated cell populations. Confirm whether the instrument provides cell diameter distribution data, which can serve as an indicator of cell health and identity.&nbsp;
&nbsp;
To discuss product availability or get selection guidance,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a><a href="https://mbpinc.net/equipments/microplate-instruments/cell-counter"></a>

Standard Test Slide for QuadCount, Pack of 1

Accuris QuadCount Automated Cell Counter

The E7500 by Accuris Instruments is the QuadCount automated cell counter with a 1 × 10⁴ to 1 × 10⁷ cells/mL counting range and three counting modes: Quick (20 sec), Normal (30 sec), and Precise (100 sec). Uses trypan blue exclusion with 4-chamber QuadSlide disposable slides; results export as PDF via USB. Touch-screen display, 115 V. Available from MBP Inc. for cell biology labs across the USA and Canada.

Optics

Pixel Resolution

Image Detection

Illumination

Sample Volume

Max. Counting Volume

Consumable Slide

Counting Modes/Speeds

Dynamic Range

Cell Size Range

Data Output

Dimensions

Weight

Electrical

Manufacture

QuadCount™ Automated Cell Counter, 115V

QuadCount™ Automated Cell Counter, 230V

50bp DNA Ladders

<h2>What is a 50 bp DNA ladder?</h2>
&nbsp;
A 50 bp DNA ladder provides reference bands spaced at 50-base-pair intervals, most commonly covering 50 bp to 1000 bp (or up to 1500 bp in some extended formats), making it suitable for gel electrophoresis experiments where samples are very small DNA fragments that a 100 bp ladder cannot resolve accurately because 100 bp band spacing is too coarse. Common applications include microsatellite and STR genotyping, analysis of small restriction digest products, multiplex or nested PCR amplicons, and other short-fragment applications in the 50&ndash;500 bp range where precise size discrimination is needed.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZR 50 bp DNA Marker for precise analysis of smaller DNA fragments&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Azura PureView&trade; 50 bp DNA Ladder for high-resolution gel results in electrophoresis
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Accuris SmartCheck&trade; 50bp DNA Ladder for size determination of PCR products
</li>
</ul>
&nbsp;
<h2>How to choose and use a 50 bp DNA ladder</h2>
&nbsp;
Use a 50 bp ladder when your fragments differ by less than 100 bp and are below 500 bp
The primary reason to use a 50 bp ladder over a 100 bp ladder is when your samples include fragments with size differences of less than 100 bp that need to be resolved, such as microsatellite alleles or closely-sized PCR amplicons from multiplex reactions.
Run small-fragment gels on 2&ndash;4% agarose or polyacrylamide for adequate resolution
High-percentage agarose (2&ndash;4%) or polyacrylamide gels are needed to resolve very small DNA fragments as distinct bands rather than all migrating together near the gel front, since standard 1% agarose does not provide sufficient resolution for fragments below approximately 200 bp.
Use a reference band at a convenient size for quick orientation
Many 50 bp ladders include a brighter reference band at a convenient, easy-to-remember size such as 250 bp or 500 bp, which serves as an anchor for identifying surrounding bands without counting from the well each time.
Confirm the 50 bp ladder's smallest band is detectable with your staining system
Very small DNA fragments, especially below 100 bp, can be difficult to detect or may run off the gel front quickly, and confirming that the ladder's smallest defined bands are visible under your staining and imaging setup helps ensure the full ladder range is usable.
Consider polyacrylamide gels for the highest resolution of fragments below 200 bp
Polyacrylamide gel electrophoresis provides substantially higher resolution than high-percentage agarose for fragments below 100&ndash;200 bp, making it the preferred format when single-base or very fine size discrimination is needed in this range, such as for SSR or STR genotyping.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Standard 50 bp DNA ladders typically cover from 50 bp to 1000 bp with bands at 50 bp intervals, and many include a brighter reference band at 250 bp or 500 bp for quick orientation. As of 2026, the 50 bp ladder remains the standard small-fragment size reference for microsatellite and STR genotyping workflows, whether on standard agarose or capillary electrophoresis platforms where defined size standards are loaded alongside samples.
<a href="https://mbpinc.net/contact-us">Contact the expert team of Molecular Biology Products Inc. (MBP)</a> and get premium-quality 50bp DNA ladders for your next experiment.

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 500 reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 1000 reactions

Accuris qMAX™ Green One-Step RT-qPCR Kit

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 100 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 500 reactions

Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 1000 reactions

Accuris qMAX™ Probe One-Step RT-qPCR Kit

40mM dNTP Mix, ready-to-use, 10mM/20μmol each, 0.5ml

40mM dNTP Mix, ready-to-use, 10mM/80μmol each, 2ml

dNTP Mix

100mM dNTP Set, dATP, dCTP, dGTP, dTTP, 250μl/25μmol each

100mM dNTP Set, dATP, dCTP, dGTP, dTTP, 1ml/100μmol each

dNTP Set

Accuris SmartCheck™ 50bp DNA Ladder, 500µl / 100 lanes

Accuris SmartCheck™ 50bp DNA Ladder, 5 x 500µl / 500 lanes

Accuris SmartCheck™ 100bp DNA Ladder, 500µl / 100 lanes

Accuris SmartCheck™ 100bp DNA Ladder, 5 x 500µl / 500 lanes

Accuris SmartCheck™ 1kb DNA Ladder, 500µl / 100 lanes

Accuris SmartCheck™ 1kb DNA Ladder, 5 x 500µl / 500 lanes

Accuris SmartCheck™ DNA Ladders

Accuris SmartCheck™ 100bp DNA Ladder

Accuris SmartCheck™ 100bp DNA Ladder (PR4010) by MBP is a ready-to-use DNA size standard for the 100 bp range with higher intensity reference bands and loading buffer included. Load 5 &mu;l per lane (100 lanes/500 &mu;l). Available as PR4010-100 (100 lanes) or PR4010-500 (500 lanes). Ships ambient; store at &minus;20&deg;C.

Protocol

Concentration

Tracking dye

Buffer formulation

Shipping and Storage

Production

Quality Control

Accuris SmartCheck™ 100bp DNA Ladder, 500ul / 100 lanes

Accuris SmartCheck™ 100bp DNA Ladder, 5 x 500ul / 500 lanes

1Kb DNA Ladders

<h2>What is a 1 kb DNA ladder?</h2>
&nbsp;
A 1 kb DNA ladder is a gel electrophoresis size standard containing a defined set of double-stranded DNA fragments spaced at or near 1-kilobase intervals, produced by digesting proprietary plasmids with restriction enzymes to generate typically 10 or more bands spanning 0.5 to 10 kb. One band, commonly at 3.0 kb, is supplied at higher concentration to serve as a bright reference band for rapid gel orientation. The 1 kb ladder is one of the most widely used size standards for routine molecular biology applications, covering the size range commonly encountered in PCR products, restriction digests, and cloned inserts.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">ZR 1 kb DNA Marker for gel electrophoresis analysis and routine DNA fragment sizing&nbsp;</li>
<li dir="ltr" role="presentation">Azura PureView&trade; 1kb DNA Ladder for the detection of DNA bands with high visibility in agarose gel systems</li>
<li dir="ltr" role="presentation">1kb Plus Opti-DNA Marker for cloning verification workflows and a wide range of DNA fragment analysis&nbsp;</li>
<li dir="ltr" role="presentation">1kb Opti-DNA Marker used in nucleic acid analysis and PCR for standard molecular weight reference&nbsp;</li>
<li dir="ltr" role="presentation">Accuris SmartCheck&trade; 1kb DNA Ladder for quality assessment of gel and DNA sizing with high precision</li>
<li dir="ltr" role="presentation">1 KB DNA Marker for routine DNA electrophoresis&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a 1 kb DNA ladder variant</h2>
&nbsp;
Choose a standard 1 kb ladder for routine PCR and restriction digest analysis
A standard 1 kb ladder with fragments spanning approximately 0.5 to 10 kb is suitable when most expected DNA fragments fall within this range, making it ideal for routine PCR verification and restriction digest analysis.
Choose a 1 kb Plus ladder when samples include smaller fragments below 500 bp
The 1 kb Plus format extends coverage down to 100 bp while maintaining the upper size range, making it useful when both small PCR products and larger DNA fragments need to be analyzed on the same gel.
Choose a 1 kb Extend ladder when samples include very large DNA fragments
The 1 kb Extend ladder expands coverage up to approximately 48.5 kb and is appropriate for large plasmids, BAC clones, and other high-molecular-weight DNA fragments analyzed on low-percentage agarose gels.
Use the recommended loading volume for accurate mass estimation
Loading the ladder according to the manufacturer's recommended amount helps ensure that any provided band mass references can be used for approximate comparison of DNA quantity based on staining intensity.
Confirm the tracking dye is compatible with your staining and imaging setup
Different ladder formulations use different tracking dyes. Some dyes may partially obscure nearby bands under UV visualization, while alternative formulations are designed to minimize interference and improve visibility across the entire ladder range.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Standard 1 kb DNA ladders are generated from defined DNA templates digested to produce fragments of known sizes. Enhanced-intensity reference bands are included to provide a readily identifiable orientation marker during gel analysis.
Ready-to-load 1 kb ladder formats with reduced-shadow tracking dyes are commonly used in routine agarose gel electrophoresis workflows because they simplify preparation and improve overall band visibility.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> to book high-quality 1Kb DNA Ladders for your lab today.

Accuris SmartCheck™ 1kb DNA Ladder

Accuris SmartCheck™ 1kb DNA Ladder (PR4100) by MBP covers 13 bands from 250&ndash;10,000 bp. Ready-to-use at 0.1 mg/ml, 5 &mu;l per lane, with loading buffer and tracking dyes included. Available in PR4100-100 (500 &mu;l / 100 lanes) and PR4100-500 (5 &times; 500 &mu;l / 500 lanes). Store at &minus;20&deg;C.

Accuris SmartCheck™ 1kb DNA Ladder, 500ul / 100 lanes

Accuris SmartCheck™ 1kb DNA Ladder, 5 x 500ul / 500 lanes

Accuris SmartCheck™ 50bp DNA Ladder

Accuris SmartCheck™ 50bp DNA Ladder (PR4005) by MBP is a ready-to-use small fragment DNA size standard with higher intensity reference bands and loading buffer included. Load 5 &mu;l per lane (100 lanes per 500 &mu;l vial). Available as PR4005-100 (100 lanes) or PR4005-500 (500 lanes). Ships ambient; store at &minus;20&deg;C.

Accuris SmartCheck™ 50bp DNA Ladder, 500ul / 100 lanes

Accuris SmartCheck™ 50bp DNA Ladder, 5 x 500ul / 500 lanes

Imaging and Gel Visualization

Product Literature

Accuris SmartDoc 2.0 Imaging Enclosure

E5001-SD by Accuris is the SmartDoc 2.0 Imaging Enclosure — a compact darkroom box that lets any smartphone capture gel images from standard UV or blue light transilluminators. The 19 × 19 cm base fits most transilluminators, accommodates gels up to 15 × 15 cm (with extender), and includes an orange photo filter for blue-light gel imaging. No dedicated imaging software required.

E5001-SmartDoc-2.0-Filter-and-User-Guide

Orange photo filter (included with E5001-SDB)

SmartDoc Photo Filter, 590nm for imaging EtBR on UV transilluminator

SmartDoc Photo Filter, 535nm for imaging green stains on UV transilluminator

Accuris SmartDoc 2.0 System Accessories

Accuris SmartDoc 2.0 System with Blue Light Illumination Base

E5001-SDB by Accuris Instruments is the SmartDoc 2.0 blue light gel documentation system with a 460 nm LED base, imaging enclosure, orange viewing cover, and orange photo filter—your smartphone serves as the camera, avoiding the cost of a dedicated imager. Maximum gel size 15 × 15 cm; universal 100–240V input.