Brands

<h2>What are Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kits?</h2>
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Spin-column environmental/microbial DNA/RNA co-extraction kits simultaneously purify genomic DNA and total RNA - including small RNAs 17-200 nt - from feces, soil, water, biofilm, swabs, plant material, and body fluids using bead-beating mechanical disruption with BashingBeads (0.1 mm + 0.5 mm) for unbiased lysis of all microbial cell types, followed by sequential Zymo-Spin column purification in a standard microcentrifuge. The ZymoBIOMICS DNA/RNA Miniprep Kit delivers DNA and RNA in separate fractions - the primary advantage over the combined total nucleic acid eluate of the MagBead format - enabling parallel metagenomic and metatranscriptomic library preparation from one undivided environmental input without post-extraction enzymatic separation steps. Choose the spin-column format when processing 1-24 environmental samples per run, when separate DNA and RNA fractions are required from the outset, or when automation infrastructure is unavailable.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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Quick-DNA/RNA&trade; Water Kit to isolate high-purity nucleic acids from water samples using specialized column purification
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Quick-DNA/RNA&trade; Pathogen MiniPrep Kit for rapid co-extraction of pathogen genomes utilizing robust spin-column technology
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ZymoBIOMICS&trade; DNA/RNA Mini Kit to ensure unbiased microbiome profile recoveries using an innovative lysis system&nbsp;
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</ul>
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<h2>How to Choose a Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kit</h2>
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Separate vs. Combined Fractions
The ZymoBIOMICS DNA/RNA Miniprep natively delivers separate DNA and RNA by column switching: gDNA binds the IIIC column while RNA flows through to the IC column. No post-extraction enzymatic treatment is needed to separate the analytes. This is the critical differentiator from the MagBead format, which requires DNase I treatment of the combined eluate to prepare an RNA fraction for RNA-seq.
PCR Inhibitor Removal Validation
Always measure A260/A230 on both eluates after extraction. Values below 1.5 indicate inhibitor carryover from humic acids (soil) or bile salts (feces) that will suppress PCR amplification and NGS library preparation. If A260/A230 &lt; 1.5, reduce input mass to 50-100 mg and add an optional pass through Zymo-Spin III-HRC Inhibitor Removal Columns on the RNA eluate before downstream use.
Bead-Beating Instrument
A vortex with a horizontal adapter (Vortex-Genie 2 at max speed x 5 min) validates for standard fecal and soil samples. A dedicated bead mill (Precellys, FastPrep-24) at 6,000-6,500 rpm for 40-60 seconds per cycle provides more consistent lysis for tough-walled Gram-positive organisms in high-diversity environmental samples; use 2-3 cycles with 30-second cooling intervals on ice to prevent RNA degradation from heat.
DNA/RNA Shield for Sample Stabilization
DNA/RNA Shield preserves microbial community RNA at room temperature for 30 days. Add Shield directly to the sample tube immediately after collection - before any transport or refrigeration delay - to capture the metabolic RNA expression state at the moment of collection. Shield-stabilized samples load directly into BashingBead Lysis Tubes without additional preparation.
Input Scaling for Low-Biomass vs. High-Biomass Samples
Standard input for feces: 250 mg. Standard input for topsoil: 200 mg. For low-biomass environmental samples (filtered water, air, oligotrophic deep soil): collect maximum biomass by filtering the largest feasible volume and add carrier RNA (5 ng per extraction) to the lysis step to improve column binding of trace nucleic acids. For high-biomass samples (feces, compost), reduce input to 50-100 mg to avoid column overloading and inhibitor carryover.
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<h2>Specifications Context</h2>
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Performance benchmarks for the ZymoBIOMICS DNA/RNA Miniprep Kit: DNA yield 2-15 ug from 250 mg feces (A260/A280 &gt;= 1.8, fragment size &gt;= 15 kb for standard input); RNA yield 2-10 ug from 250 mg feces (A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, small RNAs 17-200 nt included). The kit uses Zymo-Spin column technology with OneStep PCR Inhibitor Removal Technology validated for feces, soil, plant material, water, biofilm, swabs, saliva, and body fluids, producing inhibitor-free nucleic acids ready for RT-PCR, arrays, sequencing, and microbiome analyses. The ZymoBIOMICS DNA/RNA Miniprep is widely used in gut microbiome and soil metatranscriptomics research at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
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For bulk pricing on ZymoBIOMICS DNA/RNA Miniprep kits, bead-beating instrument recommendations, or protocol guidance for low-biomass or high-inhibitor environmental samples,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.

Spin-column

MSDS

DataSheet

Protocol

ZymoBIOMICS™ DNA/RNA Mini Kit (50 Preps.)

The ZymoBIOMICS™ DNA/RNA Miniprep Kit by Zymo Research simultaneously co-purifies DNA and total RNA (≥17 nt, including miRNA) from feces, soil, plant, and environmental samples in 50 preparations. The Zymo-Spin™ IIICG column binds up to 100 µg with a minimum 50 µl elution at A260/A280 >1.8. Also available in an RNA-only format. Kit components stored at room temperature.

Equipment

Purity

Sample Source

Size Range

Yield

Manufacturer

Shipping Condition

Storage Condition

<h2>What are microbiome standards and controls?</h2>
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Microbiome standards and controls are defined reference materials, ranging from a single microbial DNA standard to a complex mock community containing multiple known bacterial, fungal, or archaeal species at defined relative abundances, used to validate that an extraction, library preparation, and sequencing pipeline is performing as expected. Running a standard alongside real samples provides a specific, customized control for confirming reproducibility and enables meaningful comparison of results generated in different laboratories or at different times.
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<h2 dir="ltr">What you will discover:</h2>
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<ul>
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ZymoBIOMICS&trade; Community Standards: Mock communities tested for reproducibility with specific microbial ratios to assess taxonomic profiling and ensure accuracy across sequencing platforms.
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Log-Distributed DNA Standards: Customized genomic blends created to assess the limit of detection (LOD) and linear dynamic range for identifying rare taxa.
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Microbial Spike-in Controls: Internal standards with high and low loads for precise cell quantification and normalization of data across various matrices in simultaneous processing.
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Site-Targeted Reference Materials: Specialized standards for gut, oral, and fecal analysis, employing TruMatrix&trade; technology to replicate authentic spatial transcriptomics environments.
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High Molecular Weight (HMW) DNA: Ultra-pure, elongated templates designed explicitly to standardize long-read sequencing systems and verify assembly efficacy.
</li>
</ul>
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<h2>How to choose microbiome standards and controls</h2>
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Match standard complexity to your validation goal
A single-species microbial DNA or inactivated bacteria standard is sufficient for confirming basic assay sensitivity or specificity, while a multi-species mock community standard with defined relative abundances is needed to evaluate extraction and amplification bias across an entire workflow.
Use a standard to benchmark a new extraction or library prep protocol
Running a known mock community through a new or modified extraction and library preparation protocol, then comparing the resulting sequencing output against the standard's defined composition, reveals whether the new protocol introduces meaningful bias compared with an established method.
Confirm whether your standard supports both DNA-based and RNA-based workflows
Some standards are formulated specifically for DNA-based 16S or shotgun metagenomic workflows, while others, or specific lots, are validated for RNA extraction and metatranscriptomic workflows as well, a distinction worth checking against your specific application.
Include a standard when comparing results across different laboratories
Because microbiome sequencing results can be sensitive to differences in extraction method, primer choice, and sequencing platform between labs, including the same reference standard across collaborating sites supports more reliable comparison of results generated independently.
Confirm standard storage and stability before long-term use
Microbial community standards typically have documented stability windows and storage temperature requirements, and confirming these align with your intended use timeline helps avoid degraded standard material producing misleading validation results.
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<h2>Specifications context</h2>
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Individual microbial DNA and inactivated bacteria standards add value specifically by providing a specific, customized control, supporting both microbiomics and metagenomics workflows by increasing reproducibility and allowing reliable comparison of results from different laboratories. As of 2026, defined mock community standards remain a standard tool for benchmarking new extraction or library preparation protocols against established methods before adopting a new workflow for real sample processing.
<a href="https://mbpinc.net/contact-us">Reach out to the expert team at MBP Inc.</a> now to obtain a quote for the appropriate microbial standards for your lab.

Microbial Standards & Controls

MSDS (EU)

MSDS (US)

ZymoBIOMICS™ Fecal reference with TruMatrix Tech (1 ml)

The D6323 by Zymo Research is a 1 mL human fecal reference material with TruMatrix Technology, containing ~10 mg feces/mL, ~6 µg DNA/mL, and ~11 µg RNA/mL from a single homogenized batch of over 2 million identical preparations. Stored in DNA/RNA Shield with fully inactivated pathogens, D6323 serves as a reproducible positive control for 16S amplicon sequencing, metagenomics, and metatranscriptomics workflows.

Biosafety

Characterization

Concentration

Stability

Total DNA Content

Total RNA Content

ZymoBIOMICS™ Gut Microbiome Standard (750 µl)

ZymoBIOMICS Gut Microbiome Standard (D6331) by Zymo Research — 750 µl (10 preps), 21 microbial strains (Bacteria, Fungi, Archaea), ~3.94 × 10⁹ cells/ml, &lt;15% abundance deviation. BSL 1 inactivated. For gut microbiome NGS workflow validation. Stored in 2× DNA/RNA Shield. Made in the USA.

Biosafey

Impurity Level

Reference Genomes

Relative-abundance Deviation

Storage Solution

Total Cell Concentration

ZymoBIOMICS™ HMW DNA Standard (50 µl)

D6322 by Zymo Research is a high-molecular-weight (&gt;50 kb) DNA standard containing 5000 ng of genomic DNA from seven bacterial species and one yeast strain at defined theoretical proportions, designed to benchmark third-generation long-read sequencing workflows including Oxford Nanopore and PacBio platforms.

Sample Storage

Source

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
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Zymo Buffers

ZymoBIOMICS™ MagBinding Buffer (250 ml)

The D4302-2-250 by Zymo Research is the ZymoBIOMICS MagBinding Buffer in a 250 ml format, used to mediate selective nucleic acid binding to ZymoBIOMICS MagBinding Beads in high-throughput microbiomic and metagenomic DNA/RNA extraction workflows. Sold as a standalone replenishment buffer for ZymoBIOMICS MagBead Kit protocols; made in the USA, Research Use Only.

ZymoBIOMICS™ MagWash 1 (100 ml)

D4302-3-100 by Zymo Research is ZymoBIOMICS™ MagWash 1, a certified low-bioburden washing reagent supplied in 100 ml for use as the first wash step in ZymoBIOMICS DNA MagBead Kit (D4308) protocols. Its low-bioburden certification minimizes background microbial contamination in low-abundance microbiome samples.

ZymoBIOMICS™ MagWash 2 (200 ml)

D4302-4-200 by Zymo Research — ZymoBIOMICS™ MagWash 2 (200 ml), a certified low-bioburden wash buffer for the ZymoBIOMICS DNA MagBead Kits (D4308). Minimizes microbial background contamination in low-biomass microbiome samples. Room temperature storage. For 16S rRNA, metagenomics, and magnetic bead extraction workflows.

ZymoBIOMICS™ Microbial Community Standard (10 Preps.)

D6300 by Zymo Research is the ZymoBIOMICS™ Microbial Community Standard (10 preps, 0.75 ml each), containing 8 fully inactivated bacteria and 2 yeasts at ~1.4 × 1010 cells/ml with cross-validated theoretical composition and <0.01% foreign microbial DNA. Use it to benchmark 16S, ITS, and shotgun metagenomics workflows, or to detect bias in DNA isolation protocols. Store at -80°C. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

ZymoBIOMICS™ Microbial Community Standard II (Log Distribution) (750 µl)

The ZymoBIOMICS™ Microbial Community Standard II (D6310) by Zymo Research is a 750 µl (10 × 75 µl) whole-cell mock community with log-distributed abundance spanning 10 organisms across six orders of magnitude — 89.1% L. monocytogenes down to 0.000089% S. aureus (~100 cells/prep). Composition independently verified; &lt;0.01% foreign microbial DNA. For detection-limit benchmarking in 16S/metagenomics workflows.

Applicable For

Processing Volume

ZymoBIOMICS™ Oral Microbiome Standard (750 µl)

D6332 by Zymo Research — ZymoBIOMICS Oral Microbiome Standard; 12 inactivated bacterial strains; ~1.3 × 10⁹ cells/mL in DNA/RNA Shield™; &lt;0.01% foreign DNA; 10 preps × 750 µL; positive control for 16S and NGS microbiome workflows.

Impurity level

Reference genomes and 16S&18S rRNA genes

Relative-abundance deviation in average

Storage solution

Total cell concentration

<h2>What Are Spin-Column Total RNA Kits?</h2>
&nbsp;
Spin-column total RNA kits are the most widely used format for manual RNA purification in molecular biology laboratories. The technology relies on silica membrane chemistry: under chaotropic (guanidinium-based) lysis conditions, RNA adsorbs selectively to the silica membrane of the column; sequential ethanol-based wash steps remove proteins, salts, and gDNA fragments; and a low-ionic-strength buffer or RNase-free water then releases the purified RNA. Standard mini-format kits accept cell or tissue lysate from inputs of 1x10^4-1x10^7 cells or 5-100 mg tissue, bind up to 100 ug RNA, and complete the protocol in 15-30 minutes. The eluted RNA is suitable for RT-PCR, qRT-PCR, RNA sequencing, Northern blot, and hybridization without additional cleanup.&nbsp;Spin-column RNA kits are the default choice for 1-24 samples per run in any lab that does not require automated magnetic-bead processing.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Quick-RNA Microprep and Miniprep Kits for low-volume elution and highly concentrated recovery, providing ultra-clean and phenol-free transcript isolation
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Direct-zol&trade; RNA Kits to bypass tedious phase separation steps, allowing researchers to pump TRIzol-lysed samples directly into spin-columns&nbsp;
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Direct-zol&trade; DNA/RNA Parallel Modules are engineered for the non-destructive, simultaneous isolation of DNA and RNA fractions.
</li>
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ZymoBIOMICS&trade; RNA Mini Kits for uniform microbial profiling featuring host-zeroing lysis mechanics
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Quick-RNA&trade; Midiprep Assemblies to capture larger quantities of high-purity total transcripts efficiently, tailored for bulk processing&nbsp;
</li>
</ul>
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<h2>How to Choose Spin-Column Total RNA Kits</h2>
&nbsp;
Column Binding Capacity and Elution Volume
Mini-format columns bind up to 100 ug total RNA and elute in 10-100 uL. For samples with very low RNA content (needle biopsies, laser-capture microdissection sections, small cell numbers &lt;=10^4), choose micro-format columns with 8-12 uL minimum elution volume to maximize concentration. Eluting in &lt;30 uL with high RNA concentration increases sensitivity in downstream RT-qPCR.
Small RNA Retention
This is the most important differentiator between kit brands. Kits using Zymo-Spin column technology with proprietary binding buffers retain miRNA and small RNA species &gt;=17 nt in the total RNA fraction. Competing kits using standard high-ethanol wash chemistry typically lose RNA &lt;200 nt in the wash steps. If your workflow includes small RNA profiling, miRNA RT-qPCR, or small RNA-seq, verify that the kit retains &gt;=17 nt RNA before purchasing.
On-Column DNase Treatment
Included RNase-free DNase I that degrades gDNA on the column during the wash stage eliminates the need for post-elution DNase treatment. This matters for RT-qPCR with primers spanning a small intron, or for RNA-seq where even trace gDNA contamination generates false mapping reads. DNase-treated RNA typically passes a no-RT PCR control at Ct &gt;35.
Sample Type: Cells vs Tissue
Cell lysis buffer volumes and homogenization requirements differ by sample type. Cultured cells lyse directly in lysis buffer with vortexing; tissue samples require homogenization to disrupt cell structure before contact with the lysis buffer. For fibrous tissues (muscle, heart), use a bead-mill homogenizer or rotor-stator; for soft tissues (liver, brain), a disposable pestle or needle shearing is sufficient. Some kits include TissueLyser-compatible bead tubes or QIAshredder-equivalent homogenizer columns.
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<h2>Specifications Context</h2>
&nbsp;
Spin-column total RNA purity targets: A260/A280 ratio 1.8-2.1; A260/A230 ratio &gt;1.8. RNA integrity number (RIN) from fresh cultured cells or snap-frozen tissue is routinely 8-10 with validated kits. Zymo Research Quick-RNA Mini and Miniprep kits are among the most cited spin-column RNA isolation kits in published RT-PCR and RNA-seq methods; ABM RNA extraction kits offer validated protocols for bacteria, yeast, and fungi not covered by many standard cell/tissue kits. All kits supplied by MBP are Research Use Only (RUO).
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Optimized Lysis Protocol

ZymoBIOMICS™ RNA Mini Kit (50 Preps.)

ZymoBIOMICS RNA Mini Kit R2001 by Zymo Research isolates total RNA (≥17 nt) from microbiome and environmental samples in 50 preps using ZR BashingBead mechanical lysis. Accepts soil, feces, plant material, biofilms, water, and swabs; 100 µg max binding, A260/A280 &gt;1.8. All components room-temperature stable.

ZymoBIOMICS™ Spike-in Control II (Low Microbial Load)

D6321 by Zymo Research is the ZymoBIOMICS™ Spike-in Control II (Low Microbial Load). Whole-cell spike-in control with 3 bacterial strains (10³–10⁵ cells, log distribution): Truepera radiovictrix, Imtechella halotolerans, Allobacillus halotolerans. Foreign microbial DNA <0.01%. Preserved in DNA/RNA Shield™. Store at −80°C.