Brands

<h2 dir="ltr">What are cold storage sealing films?</h2>
 Cold storage sealing films are aluminum-foil or foil-laminate seals applied to microplates and deep-well storage plates to maintain an airtight barrier through long-term freezing, commonly rated to &minus;80 &deg;C and sometimes lower. Foil blocks light and gases, resists DMSO and organic solvents, and conforms to each well so the seal is visible and verifiable. Many are pierceable by pipette tips, needles, or robotic probes, and some heat-seal foils are autoclavable. Buy from this category for biobanking, compound-library archiving, and any sample set that must survive months at ultra-low temperature.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="color: #000000;" aria-level="1">
Cold storage sealing films for freezer and refrigeration use
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Aluminum foil barrier films for long-term sample protection
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
High-performance adhesive films for evaporation control
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Low-temperature resistant films for -20&deg;C to -80&deg;C storage
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Pierceable films for post-storage sample access
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Chemical-resistant sealing films for reagent stability
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Microplate sealing films for cold chain workflows
</li>
</ul>
<h2 dir="ltr"> How to choose cold storage films</h2>
 Confirm the low-temperature rating Choose a film with a stated seal-integrity range that reaches &minus;80 &deg;C; some foils hold from &minus;80 &deg;C to +110 &deg;C, covering both freezer archiving and limited thermal steps.
Decide pierceable vs permanent Pierceable foils let robotic probes or pipette tips access wells without removing the seal &mdash; essential for automated biobanks; permanent heat-seal foils give a tamper-evident, near-irreversible barrier for long-term archiving.
Check chemical resistance For DMSO compound libraries and solvent-containing samples, confirm the foil is rated DMSO- and solvent-resistant so the seal and adhesive survive storage.
Match the application method Pressure-sensitive foils apply with a hand roller; heat-seal foils need a benchtop plate sealer and a compatible hot-melt adhesive, and will not bond with standard pressure-sensitive adhesives.
<h2 dir="ltr"> Specifications context</h2>
 Cold storage foils are defined by temperature range, adhesive type, chemical resistance, and whether they are pierceable. A foil rated &minus;80 &deg;C to +110 &deg;C covers freezer archiving and limited thermal cycling, while DMSO resistance matters for screening libraries. Heat-seal foils require a matched sealer and hot-melt adhesive, whereas pressure-sensitive foils are applied by roller. Because foil is opaque, plates are identified by a label on the foil or by position in the rack rather than read through the seal, so a clear labelling convention matters for retrieval.
&nbsp;
Protect your samples with confidence. Request product options, specifications, or bulk supply details. <a href="https://mbpinc.net/contact-us" style="color: #000000;">Contact the MBP team</a> and get the right sealing solution tailored to your cold storage workflow.

Cold Storage Film

Flyer

X-Peirce 96-Well Plate Specs Sheet

X-Pierce 384-Well Plate Specs Sheet

X-Pierce™

X-Pierce™ (XP-100) by MBP is a resealable plate sealing film with X-scored well access — allows pipet tip piercing and resealing without coring or adhesive fouling. Rated &minus;40&deg;C to +90&deg;C for short-term storage and robotics. Non-sterile 96-well (XP-100, 100 films), sterile 96-well (XPS-25, 50 films), and 384-well (XP-384-50, 50 films) variants. Ships ambient.

Application

Temperature Range

Manufacturer

X-Pierce™, Short-term processing and storage at ambient temperatures; robotics, -40°C to +90°C, 100 Sealing Films/Pack

X-Pierce™, Sterile, Short-term processing and storage at ambient temperatures; robotics, -40°C to +90°C, 50/Pack, 100 Sealing Films/Pack

X-Pierce™, 384 Well

<h2 data-section-id="v1drft" data-start="0" data-end="43">What are cloning chemicals and reagents?</h2>
&nbsp;
Cloning chemicals and reagents cover the enzymes, buffers, and molecular biology-grade chemicals used in DNA fragment preparation, modification, and purification within a cloning workflow, including restriction endonucleases, T4 DNA ligase and other DNA-modifying enzymes (phosphatases, polymerases), reaction buffers, and plasmid extraction reagents. Related entities include restriction enzyme digestion, DNA dephosphorylation/phosphorylation, plasmid DNA purification (column-based and magnetic bead-based), and enzyme unit definitions.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Targeted XJa and XJb strains for faster recovery of proteins.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mix &amp; Go! solutions for optimized and dependable bacterial transformation.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Culture Media &amp; Supplements used for yeast research, and include YPD Plus and mating pheromones.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Extraction and Lysis Reagents include reliable genomic preparation and yeast protein kits.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Chromogenic Substrates and Enzymatic Inducers with highly pure X-GAL and IPTG for cloning and blue-white screening.</li>
</ul>
&nbsp;
<h2 data-section-id="1daajgc" data-start="866" data-end="913">How to choose cloning chemicals and reagents</h2>
&nbsp;
Restriction enzyme selection and buffer compatibility
Restriction enzymes require specific reaction buffers, and many modern enzyme lines use a single universal buffer compatible across multiple enzymes to simplify double-digest reactions; confirm buffer compatibility when combining enzymes from different suppliers or using older single-buffer-per-enzyme formulations.
Enzyme unit definitions and reaction scale
Restriction enzyme and ligase activity is defined in units per microliter, with one unit typically defined as the amount needed to digest or ligate a defined quantity of standard substrate DNA under specified conditions in a set time; scale enzyme quantity to the amount of DNA being processed rather than assuming a fixed volume works for all reaction sizes.
Plasmid purification grade
Plasmid DNA extraction reagents range from molecular biology grade, suitable for general cloning and sequencing, to transfection-grade or endotoxin-free formulations required for applications such as lentiviral packaging or mammalian cell transfection, where endotoxin contamination can reduce transfection efficiency or cause cell stress.
Dephosphorylation for preventing vector self-ligation
Alkaline phosphatase treatment of a linearized vector before ligation removes 5' phosphate groups, preventing vector self-ligation and improving the ratio of recombinant clones; this step is particularly useful when the vector and insert share compatible sticky ends that could otherwise religate without insert incorporation.
&nbsp;
<h2 data-section-id="1fljvas" data-start="3459" data-end="3484">Specifications context</h2>
&nbsp;
Restriction enzymes and ligases are supplied at defined unit concentrations (commonly 10&ndash;20 units per microliter for restriction enzymes), with reaction buffers formulated for specific pH and salt conditions optimized for enzyme activity. Plasmid purification kits are differentiated by yield (micrograms of DNA recovered), purity (A260/A280 ratio), and endotoxin level (endotoxin units per microgram), with endotoxin-free kits specifying levels below a defined threshold suitable for transfection applications.&nbsp;
This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and transformation kits and competent cells, used downstream of enzyme-based fragment preparation. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm enzyme buffer compatibility and plasmid purification grade for a specific downstream application before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to request a quote for high-purity chemicals and reagents.

Chemicals/Reagents

MSDS

DataSheet

Protocol

XJa (DE3) Autolysis Glycerol Stock (1 Tube)

XJa (DE3) Autolysis Glycerol Stock T5031 by Zymo Research — 1 mL glycerol stock with 500× L-arabinose — provides 80–90% cell lysis after a single freeze-thaw cycle (~10 min post-harvest). The DE3 lysogen supports T7 promoter–based recombinant protein expression; growth requires 1 mM Mg²⁺ supplementation. Storage at –70°C to –80°C. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Autolysis

Cell Growth

DNA Extraction

DNA Stability

Genotype

Processing Time

Product Storage

Protein Expression

Shipping Condition

Storage Condition

XJa Autolysis Glycerol Stock (1 Tube)

T5021 by Zymo Research is the XJa Autolysis Glycerol Stock — an EndA-/RecA- E. coli expression strain with L-Arabinose-inducible autolysis achieving 80–90% cell lysis in 10 minutes. Supplied as 1 tube with 1 ml 500× L-Arabinose. Handles plasmids up to 10 kb. Store at -70°C to -80°C. For protein expression and high-quality nucleic acid extraction.

XJb (DE3) Autolysis Glycerol Stock (1 Tube)

T5051 by Zymo Research is the XJb (DE3) Autolysis Glycerol Stock (1 ml, 1 tube), a self-lysing E. coli expression strain with DE3 T7 RNA polymerase, Lon⁻ OmpT⁻ background, and arabinose-inducible λ endolysin for 80–90% cell lysis within 10 minutes. Eliminates sonication in recombinant protein workflows. Supplied with 500× L-Arabinose. Store at -70°C to -80°C.

XJb Autolysis Glycerol Stock (1 Tube)

XJb Autolysis Glycerol Stock (T5041) by Zymo Research — arabinose-inducible autolysis E. coli strain for reagent-free recombinant protein extraction. Lon⁻, OmpT⁻. 80–90% lysis efficiency in ~10 min. 1 Tube, store at −70°C to −80°C.

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory media transfer caps
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile tubing and connector sets
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Fluid transfer adapters for cell culture vessels
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory bottle caps and closures
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile quick-connect systems
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Liquid handling accessories
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Vessel handling and support tools
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Closed-system connectors for aseptic processing
</li>
</ul>
<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
&nbsp;
Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

Product Literature

Y Shape CPC Connector for Inner Dia #3/8 Hose, Individually Wrapped, Sterile, 1/pk, 5/cs

NEST Scientific 751001: sterile Y-shape CPC connector for 3/8" ID hose in single-use bioprocess fluid systems. Splits or combines two fluid lines in a closed system. Individually wrapped, 1/pk, 5/cs. Order at <a href="https://www.mbpinc.net/quickorder">mbpinc.net/quickorder</a>.

Y-Lysis Buffer

Y-Lysis Buffer by Zymo Research is a specialized buffer for enzymatic yeast and fungal cell wall digestion, used at 25 µl per sample alongside Zymolyase in nucleic acid isolation workflows optimized for Saccharomyces cerevisiae and Candida albicans. Available in 6 ml and 100 ml formats.

Y-Lysis Buffer (100 ml)

Y-Lysis Buffer (6 ml)

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
<li dir="ltr" aria-level="1">
Targeted fluorescent labeling of specific cellular structures using viral delivery.
</li>
<li dir="ltr" aria-level="1">
Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

YFP-P2A-LOCa3 (coexpressing LOCa3, mKate2 a red fluorescent protein as marker)

Unit Quantity

<h2>What are RNA tracking dyes?</h2>
&nbsp;
RNA tracking dyes are cell-permeant, fluorogenic nucleic acid stains that selectively bind RNA over DNA, remaining virtually nonfluorescent in the unbound state and lighting up brightly once bound to RNA, which gives a clean, low-background signal for live-cell imaging. The best-characterized commercial example binds RNA with absorption and emission maxima around 490/530 nm and only weak signal when bound to DNA, validated by RNase and DNase removal experiments showing that RNase treatment eliminates nucleolar and cytoplasmic labeling while DNase treatment does not.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Biotinylated Fluorophores: High-affinity markers such as TO1, TO3, and YO3-3PEG-Biotin, engineered for robust labeling and permanent capture of RNA molecules in various structural and functional assays.
</li>
<li dir="ltr" aria-level="1">
Desthiobiotin Modifications: Specialized tools like the TO1-3PEG-Desthiobiotin Fluorophore (G7956) that allow for reversible binding and gentle elution, making them ideal for enrichment workflows where maintaining the native state of the RNA is critical.
</li>
</ul>
&nbsp;
<h2>How to choose RNA tracking dyes</h2>
&nbsp;
Confirm the dye is genuinely RNA-selective rather than a general nucleic acid stain
A true RNA-selective dye shows bright fluorescence when bound to RNA but only weak signal when bound to DNA, a distinction worth confirming against published spectral and RNase/DNase validation data before committing to a specific dye for an RNA-specific application.
Match the dye to your specific RNA visualization goal
Different dyes in a related family vary in their subcellular binding behavior, with some binding cytoplasmic RNA specifically, useful for tracking RNA granule transport in living neurons, while others are optimized for general nucleolar and cytoplasmic RNA staining.
Plan your fixation method around the dye's stated compatibility
Methanol fixation after staining generally preserves an RNA tracking dye's staining pattern with minimal loss, while formaldehyde fixation can alter the staining pattern, a distinction worth confirming before choosing a fixation protocol for downstream analysis.
Consider a dye sampler kit when working with an unfamiliar cell or tissue type
Dye staining behavior can vary across different cell and tissue types, so a sampler kit containing several related dyes lets you empirically determine which performs best for your specific sample before committing to a single dye for a full study.
Confirm buffer compatibility for the staining solution
RNA tracking dyes are generally compatible with standard physiological buffers in the pH 7.0&ndash;8.0 range, including PBS, for preparing the staining solution used in live-cell experiments.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
RNA tracking dyes have been used to stain RNA cargo in exosomes in both live-cell samples and isolated exosome preparations, and to differentiate apoptotic from non-apoptotic cells when paired with other viability indicators, reflecting the breadth of live-cell RNA applications beyond simple visualization. As of 2026, newer experimental small-molecule RNA dyes reporting higher fluorogenic enhancement and improved photostability have been described in the academic literature as potential alternatives to the long-standing commercial standard, though these remain research-stage rather than established catalog items.
Visualize your transcripts with precision&mdash;<a href="https://mbpinc.net/contact-us">contact the MBP team</a> today for a quote on our professional RNA tracking solutions.

RNA Tracking dyes

RNA Mango Insertion Guidelines

YO3-3PEG-Biotin Fluorophore

Depositor

Shipping condition

YPD Plus

Y1003 by Zymo Research is a specially formulated YPD outgrowth medium that increases yeast transformation efficiency by more than 50% compared to standard YPD. Recommended for mutant strains with poor growth characteristics, library screening, and multi-plasmid transformation experiments. Available in 50 ml (Y1003-50) and 100 ml (Y1003-100) formats.

YPD Plus (100 ml)

YPD Plus (50 ml)

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

YR Digestion Buffer (3.2 ml)

R1001-1 by Zymo Research is a 3.2 mL YR Digestion Buffer for the YeaStar RNA Kit workflow, enabling enzymatic fungal cell wall digestion before RNA isolation. Stored at room temperature, non-hazardous, and available as a standalone replacement component.

YR Lysis Buffer (6.4 ml)

YR Lysis Buffer R1001-2 by Zymo Research is a 6.4 mL guanidinium thiocyanate-based lysis reagent for total RNA isolation from yeast and fungal species including Saccharomyces cerevisiae, Candida albicans, and Aspergillus fumigatus. Used with the YeaStar RNA Kit (R1002); provides approximately 40 preparations per bottle. Sold as a standalone replacement component.