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Immortalized Cells

Thawing Protocol

Cell Line Catalog (Volume 2)

Subculturing Protocol

Freezing Protocol

T2602 Zebrafish Testicular Feeder Cells (ZtA6-3)

T2602 Zebrafish Testicular Feeder Cells (ZtA6-3) are zebrafish-derived testicular feeder cells (third clone of the ZtA6 series) used to support spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2603 Zebrafish Testicular Feeder Cells (ZtA6-4)

T2603 Zebrafish Testicular Feeder Cells (ZtA6-4) are zebrafish-derived testicular feeder cells used to support spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2604 Zebrafish Testicular Feeder Cells (ZtA6-5)

T2604 Zebrafish Testicular Feeder Cells (ZtA6-5) are the fifth clone of the ZtA6 zebrafish testicular feeder cell series, used to support spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2605 Zebrafish Testicular Feeder Cells (ZtA6-6)

T2605 Zebrafish Testicular Feeder Cells (ZtA6-6) are the sixth clone of the ZtA6 zebrafish testicular feeder cell series, supporting spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2606 Zebrafish Testicular Feeder Cells (ZtA6-7)

T2606 Zebrafish Testicular Feeder Cells (ZtA6-7) are the seventh clone of the ZtA6 zebrafish testicular feeder cell series, supporting spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2607 Zebrafish Testicular Feeder Cells (ZtA6-8)

T2607 Zebrafish Testicular Feeder Cells (ZtA6-8) are the eighth clone of the ZtA6 zebrafish testicular feeder cell series from albino zebrafish, supporting spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2608 Zebrafish Testicular Feeder Cells (ZtA6-9)

T2608 Zebrafish Testicular Feeder Cells (ZtA6-9) are the ninth clone of the ZtA6 zebrafish testicular feeder cell series, supporting spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2609 Zebrafish Testicular Feeder Cells (ZtA6-10)

T2609 Zebrafish Testicular Feeder Cells (ZtA6-10) are the tenth clone of the ZtA6 zebrafish testicular feeder cell series, supporting spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2610 Zebrafish Testicular Feeder Cells (ZtA6-11)

T2610 Zebrafish Testicular Feeder Cells (ZtA6-11) are the eleventh clone of the ZtA6 zebrafish testicular feeder cell series, supporting spermatogonial stem cell (SSC) culture in vitro. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

T2611 Zebrafish Testicular Feeder Cells (ZtA6-12)

T2611 Zebrafish Testicular Feeder Cells (ZtA6-12) are the twelfth clone of the ZtA6 series from albino zebrafish testis, requiring PriGrow XIII complex medium, Accutase passaging, gelatin coating, 38&deg;C/no CO2 incubation, and mitomycin C inactivation for feeder use. Supplied by ABM as 1&times;106 cells/1.0 ml, frozen; RUO. MTA required.

<h2>What Are DNA-Modifying Enzymes?</h2>
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DNA-modifying enzymes catalyze defined chemical changes to DNA molecules: T4 DNA Ligase seals nicks and joins fragments; T4 Polynucleotide Kinase (PNK) transfers a phosphate from ATP to 5'-OH termini; alkaline phosphatase removes 5'-phosphate groups to block self-ligation; Klenow fragment fills in 5' overhangs and removes 3' overhangs to generate blunt ends; DNA methyltransferases add methyl groups to adenine or cytosine residues for epigenetic studies or protection from restriction digestion. These enzymes are distinct from PCR polymerases (which amplify DNA) and restriction enzymes (which cleave at specific sequences). Use DNA-modifying enzymes any time you need to alter DNA end chemistry, join fragments, or protect sequences from downstream enzymatic cleavage.
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<h2 dir="ltr">What you will find:</h2>
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Thermolabile UDG: An essential "cleanup" enzyme that removes carryover contamination in PCR by breaking down uracil-containing DNA, thus guaranteeing your results stay clear of artifact-induced sequence bias.
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T4 DNA Ligase: The crucial molecular adhesive, designed for the swift and efficient connection of blunt or cohesive-end DNA pieces in cloning and NGS adapter ligation.
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T4 Gene 32 Protein: A unique single-stranded DNA binding protein that maintains open templates, greatly enhancing the efficiency of enzymes in challenging amplification and recombination tests.
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<h2>How to Choose DNA-Modifying Enzymes</h2>
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Ligation format
T4 DNA Ligase is the standard choice for cohesive-end and blunt-end ligation in cloning. For high-throughput library prep, choose ligase formulations optimized for 25 degrees C or room-temperature reaction to reduce protocol time. E. coli DNA Ligase uses NAD+ (not ATP) and is preferred for nick sealing in nick-translation reactions.
End repair requirements
Klenow fragment (3' to 5' exo-) fills 5' overhangs and removes 3' extensions to generate blunt ends required for blunt-end cloning or adaptor ligation; its 3'-&gt;5' exonuclease-minus version is used in NGS A-tailing steps to add a single 3'-dA overhang. T4 DNA Polymerase has stronger 3'-&gt;5' exonuclease activity than Klenow and is preferred for end polishing of PCR products with heterogeneous ends.
Phosphorylation/dephosphorylation
Use T4 PNK to add 5'-phosphates to PCR products or synthetic oligonucleotides before blunt-end ligation. Use alkaline phosphatase (CIP or SAP) on linearized vectors to prevent self-ligation; SAP is preferred when heat-inactivation at 65 degrees C is needed before downstream steps. Combine dephosphorylation and ligation in the same tube only after inactivating the phosphatase.
Methylation and protection
CpG methyltransferases and dam/dcm methylases protect recognition sequences from restriction cleavage or enable methylation-sensitive mapping. Dam methylase (methylates GATC sequences) and Dcm methylase (methylates CCWGG) are used to prepare templates resistant to DpnI digestion in cloning workflows where DpnI is used to remove template plasmid.
Purity certification
For RNA co-purification or RNA-sensitive workflows, confirm all DNA-modifying enzymes carry RNase-free certification. ABM lot-release QC includes RNase and DNase contamination tests; certificates of analysis are available on request through MBP.
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<h2>Specifications Context</h2>
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T4 DNA Ligase activity is defined as the amount of enzyme catalyzing 50% ligation of HindIII-cut lambda DNA fragments in 30 minutes at 16 degrees C in a 20 microliter reaction; 1 Weiss unit equals approximately 60 cohesive-end units. Optimal blunt-end ligation uses PEG 4000 at 15% (w/v) as a molecular crowding agent and runs at 25 degrees C to increase effective enzyme concentration. Streamlined NGS library prep workflows combine end repair, A-tailing, and adaptor ligation into a single-tube step, reducing total hands-on time to under 1 hour.&nbsp;
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Seize control of your DNA processes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> now for a quote on our premium DNA-altering enzymes.