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Universal Centrifuge Accessories

<h2>What Are Universal Centrifuge Accessories?</h2>
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Universal centrifuge accessories are the interchangeable components that determine the range of applications a universal centrifuge can perform. These include swing-out rotor buckets for blood tubes, conical tubes, microplates, and large bottles; fixed-angle rotors for high-speed pelleting; hematocrit and cytospin rotors; tube adapters; and aerosol-tight bio-containment lids. Selecting the appropriate accessories allows a single centrifuge to support multiple laboratory workflows efficiently and safely.
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<h2 dir="ltr">What you will find:</h2>
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Tube adapters and inserts for compatibility with multi-volume samples&nbsp;
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Fixed-angle and swing-out rotors for varied centrifugation applications
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Lids and sealed buckets for safe and reliable operations
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PCR adapters and microplate carriers for high-throughput workflows
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Centrifuge tubes, holders, and supporting aids for efficient and complete setups
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<h2>How to Choose Universal Centrifuge Accessories</h2>
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Swing-Out Bucket Selection by Application
Choose swing-out buckets according to the sample formats used in your laboratory. Blood tube buckets support routine clinical applications, conical tube buckets are suitable for cell culture and molecular biology, microplate carriers enable high-throughput workflows, and large-bottle carriers accommodate large-volume sample processing.
Fixed-Angle Rotors
Fixed-angle rotors provide higher centrifugal forces for microtube, PCR tube, and small-volume sample processing. Ensure that both the rotor and centrifuge motor specifications support the required operating speeds and sample formats.
Cytospin Clip Rotors
Cytospin rotors are designed for preparing microscope slides from low-cellularity samples such as body fluids. Verify compatibility with the required funnel sizes and use bio-containment features when processing potentially infectious materials.
Bio-Containment Lids
Aerosol-tight lids help contain biological samples during centrifugation and support safe laboratory practices. Regular inspection of sealing components and O-rings ensures reliable containment and long-term performance.
Reducing Adapter Inserts
Adapter inserts allow smaller tubes to fit securely within larger bucket positions. Using properly matched adapters improves balance, prevents movement during operation, and helps maintain consistent centrifugation results.
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<h2>Specifications Context</h2>
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Modern universal centrifuge systems support interchangeable accessory configurations that enable rapid transitions between clinical, research, cell culture, and cytology workflows. Swing-out buckets, fixed-angle rotors, and microplate carriers provide flexibility while maintaining compatibility with a broad range of vessel formats.
Routine inspection and maintenance of accessories, including adapters, lids, and sealing components, help ensure safe operation and extend equipment lifespan. Advances in bio-containment systems and modular rotor designs continue to improve laboratory efficiency and versatility.
Time to upgrade your universal centrifuge accessories. <a href="https://mbpinc.net/contact-us">Contact the expert team </a>of Molecular Biology Products Inc. (MBP) and the find perfect match today.

50ml conical self-standing insert for Z327-200-BUK, 2/pk

Z327-200-A50SS by Hermle (2/pk) is a skirted/self-standing 50 ml tube insert for the Z327-200-BUK bucket in the Z327-200 swing-out rotor, compatible with Z307, Z327, and Z327-K centrifuges at up to 5,000 RPM / 4,136 × g. Tube OD 29 mm, length 95–105 mm.

Manufacture

50ml round bottom insert for Z327-200-BUK, 2/pk

Z327-200-A50R by Benchmark Scientific is a 50 ml round-bottom tube insert for the Z327-200-BUK swing-out bucket on Hermle Z307 and Z327 centrifuges, sold 2 per pack. Supports round-bottom 50 ml tube format within the 200 ml bucket.

50ml round insert for 85ml rotor, 2/pk.

Z326-85-A50R by Hermle/Benchmark Scientific is a 50 ml round tube insert for the 6×85 ml fixed-angle high-speed rotor (Z326-0685-25), sold 2 per pack. Enables 50 ml round-bottom tube runs on Z326, Z326-K, and Z366 centrifuges at up to 11,000 rpm / 13,932 ×g (Z326) or 13,500 rpm / 20,984 ×g (Z326-K).

50ml round insert for Z306-100 2/pk.

Z306-100-A50R by Benchmark Scientific (Hermle) is a 2-pack of 50 ml round-bottom tube inserts for the Hermle Z306-100 swing-out rotor, compatible with Hermle Z306 and Z326 Universal Centrifuges. These inserts adapt the Z306-100 bucket to accept 50 ml round-bottom tubes for high-volume cell biology and sample preparation workflows.

50ml round inserts for Z326-100H rotor, 2/pk.

Z326-100H-A50R by Hermle / Benchmark Scientific provides 50&nbsp;ml round-bottom tube inserts for the Z326-100H hermetically sealed swing-out rotor on the Hermle Z306 and Z326 Universal Centrifuges, sold 2 per pack. These inserts enable aerosol-contained centrifugation of 50&nbsp;ml round-bottom samples — suited for infectious material, clinical specimens, and biosafety-required workflows.

50ml self standing, insert for 85ml rotor, 2/pk.

Z326-85-A50SS by Benchmark Scientific is a self-standing 50 ml conical tube insert for the 85 ml fixed angle rotor used with Hermle Z326, Z366, and compatible centrifuges, supplied as a pack of two. The self-standing design keeps 50 ml conicals stable on the bench before and after centrifugation. Order via mbpinc.net/quickorder using catalog number Z326-85-A50SS or email customerservice@mbpinc.net.

Erlenmeyer Flasks Accessories

<h2 dir="ltr">&nbsp;What are Erlenmeyer Flask Accessories?</h2>
 Erlenmeyer flask accessories are the replacement caps, transfer cap assemblies, vent filters, and adapter fittings used alongside PC and PETG shake flasks to customize gas exchange, enable closed-system liquid transfer, and replace consumable caps between culture runs. Accessory selection determines whether a shake flask acts as a standard culture vessel, a sealed storage container, or a closed-system liquid transfer assembly in a cGMP workflow.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Accessories compatible with high-efficiency Erlenmeyer flasks in 2 L, 3 L, and 5 L formats.</li>
<li>Available in bi-directional, inverted, and multifunctional transfer cap configurations.</li>
<li>Supports both standard-neck and wide-mouth 3 L flask designs.</li>
<li>Includes options with integrated silicon tubing for transfer applications.</li>
<li>Enables customization of flask workflows through interchangeable transfer cap accessories.</li>
</ul>
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">How to Choose Erlenmeyer Flask Accessories</h2>
 Vented Caps: Standard vs. Oversized Membrane Standard vented Erlenmeyer caps have a 0.22 &micro;m PTFE membrane in a small-diameter HDPE housing. NEST Scientific vented caps feature an oversized membrane area that increases the gas exchange surface, supporting higher oxygen mass transfer for dense suspension cultures at elevated shaker speeds. At 150 RPM with 20&ndash;25% fill, NEST oversized vented caps provide sufficient oxygen transfer for mammalian suspension cultures at densities up to approximately 5 &times; 10⁶ cells/mL. For cultures targeting densities above 10 &times; 10⁶ cells/mL in a standard shake flask format, a sparged bioreactor system is more appropriate. Plug Seal Caps: Autoclave vs. Non-Autoclave HDPE plug seal caps for NEST PC Erlenmeyer flasks are compatible with autoclave cycles at 121 &deg;C when loosened 1&ndash;1.5 turns before autoclaving to prevent steam pressure buildup. Plug seal caps for NEST PETG flasks are compatible with static incubation and transport but not autoclaving (PETG maximum temperature: +60 &deg;C). Confirm whether your accessory cap is rated for autoclave use before including it in a steam sterilization cycle. Transfer Cap Assemblies for Closed-System Culture NEST customized transfer cap assemblies combine an Erlenmeyer-compatible cap body with a bi-directional transfer port, TPE tubing, and a Luer lock or CPC connector &mdash; enabling aseptic inoculation, sampling, or media addition to a running shake flask culture without opening the flask. This closed-system capability is used in cGMP process development, GMP seed train management, and contamination-critical research workflows. Transfer caps are available in sizes matching NEST 125 mL, 250 mL, 500 mL, 1,000 mL, 2,000 mL, and 3,000 mL flask neck formats. Cap Sizing and Flask Compatibility Erlenmeyer flask caps are sized to the neck thread diameter of the flask. NEST Scientific PC and PETG Erlenmeyer flasks use proprietary screw-thread neck dimensions &mdash; confirm the specific cap catalog number matches your flask's neck size before ordering replacement caps. Do not mix cap brands across flask brands without verifying thread compatibility; mismatched caps create gas leaks at the vent membrane, contaminating cultures. Replacement Cap Frequency for Reusable PC Flasks For reusable PC flasks, replace the vented cap after each culture run to prevent membrane clogging from media splatter, condensation, and biofilm formation on the PTFE membrane. Reusing a contaminated or clogged vent membrane reduces gas exchange efficiency, creating a DO deficit that depresses cell growth without a visible contamination event. NEST vented caps for PC flasks are available in bulk packs for cost-efficient per-run replacement. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific Erlenmeyer flask accessories &mdash; including replacement vented caps, plug seal caps, and transfer cap assemblies &mdash; are manufactured from USP Class VI HDPE (cap housing) and USP Class VI PTFE (0.22 &micro;m membrane), with TPE tubing in transfer assemblies. All accessory caps are sterile and non-pyrogenic per the lot certificate. The 0.22 &micro;m membrane pore size is the standard for microbial retention in cell culture venting applications. Transfer cap assemblies compatible with the NEST Erlenmeyer flask line are available in sizes for 125 mL through 3,000 mL flasks, individually wrapped in sterile packaging. NEST Scientific is ISO 9001:2015 and ISO 13485 certified.&nbsp; 
Power your scientific breakthroughs with high-precision labware.&nbsp;<a href="https://mbpinc.net/contact-us" title="the MBP team" style="color: #236fa1;">Contact the MBP team</a> today for bulk availability, technical specifications, and expert support.

Erlenmeyer Flask Accessories

DataSheet

5L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap

NEST Scientific C10422-AZB090B is a sterile bi-directional transfer cap for 5 L High Efficiency Erlenmeyer Flasks — 90 cm TPE tube (1/8″ ID × 1/4″ OD), 0.22 μm vent filter (Φ24 mm), supports both media addition and culture drain through a single closed-system connection. Sold without flask. 1/pk, 4/cs.

Manufacturer

5L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap with TPE Tube (90cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap with TPE Tube (50cm 1/4" ID 3/8" OD), Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (90cm1/4" ID 3/8" OD), Male CPC Connector with Female Sealing Cap with, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Bi-directional Transfer Cap with Silicon Tube (90cm1/4" ID 3/8" OD), Female CPC Connector with Male Sealing Cap with, Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Inverted Transfer Cap

The C10431-AZC090B by NEST Scientific is an inverted transfer cap for the 5 L NEST High Efficiency Erlenmeyer Flask, enabling closed-system liquid transfer that minimizes external contamination risk during media handling and bioprocess operations. Features a 90 cm transfer tube, 0.22 µm vent filter, and ships sterile to SAL 10⁻⁶. Packed 1 per pack, 4 per case.

5L High Efficiency Erlenmeyer Flask Inverted Transfer Cap with TPE Tube (90cm 1/8" ID 1/4" OD), Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Inverted Transfer Cap with TPE Tube ( 90cm 1/4" ID 7/16" OD), Vent Filter (0.22 μm Φ42mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap

C10411-AJE092B by NEST Scientific is a sterile multifunctional transfer cap for 5 L High Efficiency Erlenmeyer Flasks, with 92 cm weldable TPE tubing (1/8" ID × 1/4" OD), sampling nozzle with one-way valve, and a 0.22 µm vent filter (Φ50 mm). Enables closed-system liquid transfer and aseptic sampling. SAL 10⁻⁶, endotoxin-free, animal-origin-free; 1/pk, 4/cs.

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with TPE Tube (92cm 1/8" ID 1/4" OD) and a Sampling Nozzle, Vent Filter (0.22 μm Φ50mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with TPE Tube ( 90cm 1/4" ID 7/16" OD), Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

5L High Efficiency Erlenmeyer Flask Multifunctional Transfer Cap with Silicon Tube (100cm 3/16" ID 3/8" OD), Male CPC Connector with Female Sealing Cap, Vent Filter (0.22 μm Φ24mm), without Flask, Sterile, 1/pk, 4/cs

<h2>What are ChIP buffers?</h2>
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ChIP buffers are the aqueous formulations used at each stage of a chromatin immunoprecipitation protocol: cell lysis buffer disrupts the plasma membrane and releases nuclear content, nuclear lysis buffer solubilizes chromatin under conditions compatible with subsequent immunoprecipitation, sonication buffer maintains chromatin stability during shearing, ChIP dilution buffer reduces SDS concentration from the lysis buffer to allow antibody-antigen binding, sequential wash buffers with increasing ionic strength and detergent remove non-specifically bound chromatin while preserving the specific antibody-antigen-DNA complex, and elution buffer releases the captured complex from the antibody-bead resin before de-crosslinking.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">MN Digestion Buffer used in MNase-based workflows for enzymatic fragmentation of&nbsp; chromatin&nbsp;</li>
<li dir="ltr" role="presentation">Chromatin Shearing Buffer using sonification for chromatin disruption and preparation</li>
<li dir="ltr" role="presentation">5X MN Stop Buffer for the termination of enzymatic digestion reactions</li>
<li dir="ltr" role="presentation">Chromatin Dilution Buffer used in ChIP assays to provide optimized conditions for antibody binding&nbsp;</li>
<li dir="ltr" role="presentation">Chromatin Wash Buffer I, II, and III for background reduction and step-by-step non-specific binding removal&nbsp;</li>
<li dir="ltr" role="presentation">ChIP DNA Binding Buffer for purification and capture of immunoprecipitated DNA</li>
<li dir="ltr" role="presentation">5X Chromatin Elution Buffer for protein&ndash;DNA complexes recovery</li>
<li dir="ltr" role="presentation">5M NaCl for enhancing elution and chromatin stabilization</li>
<li dir="ltr" role="presentation">Nuclei Prep Buffer used before chromatin extraction for superior nuclear isolation</li>
</ul>
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<h2>How to use ChIP buffers</h2>
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Always add protease inhibitors to lysis and wash buffers immediately before use
Protease inhibitors must be added fresh to cell lysis and nuclear lysis buffers immediately before use, since most inhibitors are unstable in aqueous solution and lose activity quickly, and without them, nuclear proteases released during lysis will degrade histone tails and reduce ChIP enrichment of histone modifications.
Confirm SDS concentration is diluted before immunoprecipitation
High SDS in the nuclear lysis buffer is necessary for chromatin solubilization and sonication, but must be diluted approximately 10-fold using ChIP dilution buffer before antibody addition, since high SDS disrupts antibody-antigen binding and reduces immunoprecipitation efficiency.
Keep all buffers and chromatin on ice throughout the workflow after lysis
Chromatin prepared for ChIP is sensitive to non-specific interactions and protease activity after cell lysis, and maintaining samples at 4&deg;C throughout all lysis, sonication, immunoprecipitation, and wash steps minimizes these effects and preserves specificity of the enrichment.
Optimize wash buffer stringency to balance specificity against yield
ChIP wash buffers are applied sequentially at increasing ionic strength and detergent concentration to remove non-specifically bound chromatin; conditions too stringent can also strip the specific antibody-antigen-DNA complex from the beads, reducing yield, so optimization of wash conditions for each antibody-target combination improves performance.
Confirm elution buffer composition fits your downstream application
Standard ChIP elution buffers containing SDS and sodium bicarbonate release the protein-DNA complex and reverse crosslinks, but high SDS in the eluate can inhibit some NGS library preparation enzymes, making a DNA cleanup step after elution important before proceeding to library prep.
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<h2>Specifications context</h2>
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Protease inhibitor cocktails, added fresh to lysis buffers immediately before ChIP, are a standard component across kit formats and in-house protocols, since even brief protease activity can compromise histone modification detection by degrading the histone tails that ChIP antibodies recognize. As of 2026, ChIP buffer formulations compatible with low-input chromatin and NGS library preparation continue to be optimized for primary cell and tissue ChIP workflows.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP and request a quote today to get high-quality ChIP buffers for your lab.

Buffers

MSDS

5M NaCl (1 ml)

D5210-7-1 by Zymo Research is 5&nbsp;M NaCl solution, 1&nbsp;ml, a component of the Zymo-Spin ChIP Kit (D5210). Used in high-salt wash steps during chromatin immunoprecipitation (ChIP) protocols. Available separately for component replenishment.

Shipping Condition

Storage Condition

5X Chromatin Elution Buffer (10 ml)

D5210-6-10 by Zymo Research is a 5× concentrated Chromatin Elution Buffer (10 mL) used for sample processing in chromatin immunoprecipitation (ChIP) assays performed with the Zymo-Spin ChIP Kit.

5X MN Stop Buffer (6 ml)

D5220-4 by Zymo Research is the 5X MN Stop Buffer (6 ml), used with the EZ Nucleosomal DNA Prep Kit to terminate micrococcal nuclease digestion and preserve nucleosomal DNA fragment length for ChIP-seq, ATAC-seq, and related chromatin analysis workflows. Ordered as a standalone replacement when this buffer component is depleted.