Brands

<h2 dir="ltr">What are Cell Culture Flasks?</h2>
 Cell culture flasks (T-flasks) are sterile, rectangular polystyrene vessels with a canted, angled neck designed to house adherent or suspension cell cultures in a CO₂ incubator. They are available in growth surface areas from T25 (25 cm&sup2;) to T225 (225 cm&sup2;), with either tissue culture&ndash;treated (TC-treated) surfaces for adherent cells or non-treated surfaces for suspension and microbial culture. Cap options include vented caps with 0.2 &micro;m hydrophobic membranes for CO₂ gas exchange, and plug seal caps for anaerobic or sealed transport.&nbsp; Choose the flask size based on the number of cells needed for downstream applications: T25 for seed stocks, T75 for routine maintenance, T175 and T225 for scale-up and protein production runs.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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<a href="cell-culture-flasks/treated-cell-culture-flasks" title="Treated Cell Culture Flasks" style="color: #236fa1;">Treated Cell Culture Flasks</a>: Vacuum-plasma modified surfaces that enhance hydrophilic interaction for uniform monolayer formation.
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<li dir="ltr" aria-level="1">
<a href="cell-culture-flasks/non-treated-cell-culture-flasks" title="Non-Treated Cell Culture Flasks:">Non-Treated Cell Culture Flasks:</a> Naturally hydrophobic polystyrene designed specifically for hybridomas and non-adherent suspension cultures.
</li>
</ul>
<h2 dir="ltr"> How to Choose Cell Culture Flasks</h2>
 Size by Growth Area and Working Volume T25 flasks (25 cm&sup2;, working volume 5&ndash;10 mL) are used for seed stocks, small-scale experiments, and initial passages of new cell lines where preservation of limited cells is critical. T75 flasks (75 cm&sup2;, 15&ndash;25 mL) are the routine maintenance format for most adherent cell lines in research settings. T175 flasks (175 cm&sup2;, 35&ndash;50 mL) suit scale-up for protein production, viral vector manufacturing, and high-yield transient transfection. T225 flasks (225 cm&sup2;, 50&ndash;70 mL) provide maximum single-flask growth area before scale-up to multi-layer vessels. TC-Treated vs. Non-Treated Surface TC-treated flasks (vacuum plasma treated, contact angle &lt;10&deg;) are essential for adherent mammalian cell lines &mdash; HeLa, HEK293, CHO (adherent), Vero, A549, NIH 3T3, and primary cells, including fibroblasts, epithelial cells, and endothelial cells. Non-treated flasks maintain the original hydrophobic PS surface, preventing adherent growth &mdash; used for suspension CHO, Jurkat, K562, hematopoietic cells, hybridomas, Sf9/Hi5 insect cells, and bacterial culture. Canted vs. Straight Neck A canted (angled) neck on the flask allows the flask to rest at an angle when standing, keeping the growth surface slightly tilted during feeding and pipetting. This angled geometry improves access for serological pipettes and reduces the risk of contaminating the culture surface during media changes. All NEST Scientific T-flasks use a canted neck design. Cap Type and Gas Exchange Vented caps with 0.2 &micro;m hydrophobic membranes are the standard for CO₂ incubator use. The 0.2 &micro;m pore size blocks microbial contamination while allowing CO₂ and O₂ to equilibrate freely, maintaining medium pH through the bicarbonate buffering system. Plug seal caps isolate the flask from the ambient atmosphere &mdash; used for short-term transport, sealed storage, and anaerobic culture applications. Some protocols require alternating between vent and plug seal during different culture phases. Sterility and Traceability All NEST Scientific cell culture flasks carry E-beam sterilization (ISO 11137 equivalent SAL 10⁻⁶), DNase/RNase-free and pyrogen-free lot certification, batch traceability codes, and are produced under ISO 9001:2015 and ISO 13485 quality management. Flasks are individually packaged in peelable Tyvek sleeves or resealable sterile bags. Each flask features two large labeling areas for cell line identification and passage number notation.
<h2 dir="ltr">&nbsp;</h2>
<h2 dir="ltr">Compare Cell Culture Flask Sizes </h2>
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<ul>
<li>T25: Growth Area: 25 cm&sup2;; Working Volume: 5&ndash;10 mL; Typical Cell Yield (adherent): 5&ndash;8 &times; 10⁶; Best Application: Seed stock, new cell line setup.</li>
<li>T75: Growth Area: 75 cm&sup2;; Working Volume: 15&ndash;25 mL; Typical Cell Yield (adherent): 15&ndash;25 &times; 10⁶; Best Application: Routine passage and maintenance.</li>
<li>T175: Growth Area: 175 cm&sup2;; Working Volume: 35&ndash;50 mL; Typical Cell Yield (adherent): 35&ndash;60 &times; 10⁶; Best Application: Scale-up, transient transfection.</li>
<li>T225: Growth Area: 225 cm&sup2;; Working Volume: 50&ndash;70 mL; Typical Cell Yield (adherent): 50&ndash;80 &times; 10⁶; Best Application: High-yield expansion, virus production.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture flasks (T25 catalog 707001, T75 catalog 708001, T175 catalog 709001, T225 catalog 710001) are manufactured from 100% virgin polystyrene in an ISO 8 (Class 10,000) cleanroom. Vacuum plasma TC treatment creates a surface contact angle of &lt;10&deg; (hydrophilic) per NEST lot data, verified by water contact angle goniometry. Vented caps contain a hydrophobic 0.2 &micro;m PTFE membrane that passes the USP Particulate Standard for injectable preparations &mdash; confirmed free of fibers and particulates at lot release. Flask footprints are standard for incubator shelf spacing: T25 width 100 mm, T75 width 150 mm, T175/T225 width 167 mm. As of 2026, NEST Scientific flasks are stocked by MBP for same-case reorder by PO. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center. 
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Eliminate variables in the incubator to ensure absolute integrity in your results with MBP's Cell Culture Flasks! <a href="https://mbpinc.net/contact-us">Reach out to our team</a> today for volume-based pricing.

Cell Culture Flasks

<h2 dir="ltr">What are TC-Treated Cell Culture Flasks?</h2>
 Tissue culture&ndash;treated (TC-treated) cell culture flasks are sterile polystyrene T-flasks whose inner growth surface has been modified by vacuum plasma treatment to introduce negatively charged, oxygen-containing functional groups (carboxyl, carbonyl, hydroxyl) that reduce the water contact angle from ~75&ndash;90&deg; (hydrophobic, untreated PS) to below 10&deg; (hydrophilic). This surface modification directly promotes cell attachment, spreading, and cytoskeletal organization in adherent mammalian cell lines. Without TC treatment, virtually all primary mammalian cells and established adherent lines, including HeLa, HEK293 (adherent), CHO-K1, Vero, A549, NIH 3T3, MDCK, and primary fibroblasts, fail to attach and undergo anoikis within 24&ndash;48 hours. TC-treated flasks are the correct default for any adherent mammalian cell line unless the experimental protocol explicitly specifies non-treated or specialized surfaces (ultra-low attachment, poly-D-lysine, collagen I).
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<h2 dir="ltr">What you will find:</h2>
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<ul>
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TC-Treated Surfaces: Vacuum-plasma modified polystyrene for maximum cellular adhesion and uniform growth kinetics.
</li>
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Gas Exchange Efficiency: Vented caps with 0.22&micro;m hydrophobic membranes ensure sterile CO2 exchange while preventing contamination.
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Scalable Geometry: Available in T25, T75, T175, and T225 formats to support both routine maintenance and high-yield production.
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Advanced Ergonomics: The angled neck design facilitates complete access for cell scrapers and serological pipettes, minimizing media waste.
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Optical Excellence: USP Class VI high-clarity material provides distortion-free microscopy for precise monitoring.
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Certified Purity: Validated RNase, DNase, and Pyrogen-free to ensure experimental data integrity.
</li>
</ul>
<h2 dir="ltr"> How to Choose TC-Treated Cell Culture Flasks</h2>
 Size by Growth Area and Workflow Stage T25 (25 cm&sup2;, 5&ndash;10 mL): seed stocks, low-passage critical cell lines, newly thawed cells, and experiments requiring fewer than ~8 &times; 10⁶ cells. T75 (75 cm&sup2;, 15&ndash;25 mL): the standard research-lab flask for routine weekly passage of most adherent cell lines; the most commonly stocked format. T175 (175 cm&sup2;, 35&ndash;50 mL): scale-up for transient transfection (lipofection, calcium phosphate, PEI), viral vector production (lentivirus, AAV), and protein expression experiments requiring 35&ndash;60 &times; 10⁶ adherent cells per flask. T225 (225 cm&sup2;, 50&ndash;70 mL): maximum single-flask density before transitioning to multi-layer BioFactory vessels; used for large-scale CHO adherent culture and high-yield antibody or virus production runs. Vented vs. Plug Seal Cap Vented caps with a 0.2 &micro;m hydrophobic PTFE membrane allow free CO₂/O₂ gas exchange &mdash; mandatory for cells buffered by the bicarbonate/CO₂ system in a CO₂ incubator (typically 5&ndash;10% CO₂). Plug seal caps create a sealed environment; use them for short-term transport between incubators, for HEPES-buffered media that do not require CO₂, or for anaerobic culture. Using a plug seal cap in a standard CO₂ incubator blocks gas exchange and acidifies the medium within 2&ndash;4 hours. Straight vs. Canted Neck Straight-neck flasks are the traditional geometry; canted (angled) neck flasks tilt the growth surface away from the neck, improving pipette access during media changes and cell harvesting. NEST Scientific uses a canted neck design across all flask sizes. Confirm your biosafety cabinet arm reach and pipette length before committing to one geometry in bulk. Lot-to-Lot Consistency For long-running experiments, drug screening, or validated bioprocess workflows, lot-to-lot TC treatment consistency is critical. NEST Scientific verifies contact angle on each manufacturing lot. Switching the flask lots mid-experiment can alter cell attachment density, proliferation rate, and morphology. Order sufficient flasks from a single lot for the duration of a defined experimental series. Cells That Require TC-Treated Surfaces HeLa, A549, Vero (BSC-1), HEK293 (adherent), CHO-K1 (monolayer), MDCK, NIH 3T3, MCF-7, U2OS, PC-3, HepG2, primary dermal fibroblasts, primary endothelial cells, primary epithelial cells, and most primary culture preparations require TC-treated surfaces. Cells that must NOT be cultured on TC-treated surfaces include Jurkat, K562, HL-60, suspension CHO-S, hybridomas, Sf9/Hi5 insect cells, and hematopoietic progenitor cells &mdash; use non-treated flasks for all of these. 
<h2 dir="ltr"> Specifications Context </h2>
 NEST Scientific TC-treated flasks are manufactured from 100% virgin polystyrene in ISO 8 (Class 10,000) cleanrooms. Vacuum plasma TC treatment is verified by water contact angle goniometry per manufacturing lot; contact angle &lt;10&deg; is the release criterion. E-beam sterilization achieves SAL 10⁻⁶; flasks are DNase/RNase-free and non-pyrogenic per lot certificate. Vented cap 0.2 &micro;m PTFE hydrophobic membrane is certified free of particles per USP Particulate Standard. Flask footprint dimensions comply with standard CO₂ incubator shelf spacing: T25 (100 mm width), T75 (150 mm), T175/T225 (167 mm). Double-layered Tyvek packaging maintains sterility until the point of use. NEST Scientific holds ISO 9001:2015, ISO 13485, and ISO 11137 certifications, and has FDA registration and CE marking. As of 2026, MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
 <a href="https://mbpinc.net/contact-us">Connect with the helpful team at MBP </a>today to secure a customized quote for your facility.

Treated Cell Culture Flasks

SPL Cell Culture Flask, with Filter or Plug Cap

SPL PS TC-treated cell culture flasks — 25, 75, and 175 cm² in filter cap (vented) and plug cap (sealed) configurations. Sterile to SAL 10⁻⁶, angled wide-neck design, excellent stackability. 5 flasks/bag.

Tissue Culture Flask with Plugged Cap, PS, 25cm2, TC treated, Sterile to SAL10-6, 5/bag, 40bags/case

Tissue Culture Flask with Plugged Cap, PS, 75cm2, TC treated, Sterile to SAL10-6, 5/bag, 20bags/case

Tissue Culture Flask with Plugged Cap, PS, 175cm2, TC treated, Sterile to SAL10-6, 5/bag, 8bags/case

Cell Culture Flask with Filter Cap,PS, 25cm2, TC treated, Sterile to SAL10-6. 5 Flasks/Bag, 40 Bags/Case

Cell Culture Flask with Filter Cap,PS, 75cm2, TC treated, Sterile to SAL10-6. 5 Flasks/Bag, 20 Bags/Case

Cell Culture Flask with Filter Cap,PS, 175cm2, TC treated, Sterile to SAL10-6.  5 Flasks/Bag, 8 Bags/Case

<h2 dir="ltr"> What Are Serological Pipettes?</h2>
 Serological pipettes are calibrated, graduated, sterile polystyrene disposable pipettes used with a motorised pipette controller to transfer measured volumes of liquid in the 1&ndash;100 mL range. They are the primary tool for cell culture media transfer, buffer preparation, reagent dispensing in volumes above 1 mL, and serial dilutions requiring volumetric accuracy in the millilitre range. They differ from aspirating pipettes &mdash; which have no graduations and are used only for vacuum-assisted waste removal &mdash; and from microcentrifuge tubes, which are not dispensing instruments. Serological pipettes are the correct format for any measured liquid transfer &ge;1 mL in cell culture or reagent preparation; pair them with a motorised pipette controller for safe, ergonomic operation. 
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">1 mL to 50 mL Serological Pipettes &ndash; Standard range for routine and high-volume liquid transfer</li>
<li dir="ltr" role="presentation">Glass Serological Pipettes &ndash; Reusable options for solvent resistance and durability</li>
<li dir="ltr" role="presentation">Sterile Serological Pipettes &ndash; Gamma-sterilized for aseptic laboratory use</li>
<li dir="ltr" role="presentation">Non-Sterile Serological Pipettes &ndash; Cost-effective option for general applications</li>
<li dir="ltr" role="presentation">Graduated Serological Pipettes &ndash; For accurate volume measurement and dispensing </li>
</ul>
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<h2>How to Choose Serological Pipettes</h2>
 Volume: Standard Sizes and ISO 7550 Colour Coding ISO 7550 assigns a specific colour code to each serological pipette volume: 1 mL = red; 2 mL = yellow; 5 mL = blue; 10 mL = orange; 25 mL = green; 50 mL = purple (pink/violet depending on supplier). Colour coding allows quick visual volume identification when multiple sizes are in use simultaneously in a BSC. Packaging: Individual Wrap vs Bulk Individually wrapped sterile pipettes are required for cell culture, molecular biology, and aseptic technique. Plastic/plastic peel-open packaging eliminates paper particulate from paper/plastic wrappers &mdash; important in sensitive cell culture applications and for RNA work. Bulk-packed (canister or bag) formats are cost-effective for non-sterile applications such as buffer preparation and reagent dispensing in fume hoods. Construction: 1-Piece vs 3-Piece 1 mL, 2 mL, and 5 mL serological pipettes are typically one-piece moulded polystyrene constructions. 10 mL, 25 mL, and 50 mL pipettes use 3-piece ultrasonically welded construction (Globe Scientific) for the body-to-cone and body-to-cap joints &mdash; essential for the structural integrity of larger formats under aspiration vacuum. Tip and Graduation Features Negative graduations (markings above the nominal maximum) allow volumes slightly above nominal to be read. Fine-point tips with slow drip-off improve pipetting accuracy during cell culture media transfers. Some brands offer Wobble-not-style pipettes with reduced barrel flex for easier single-hand operation. Filter Plug Material Cotton filter plugs are standard; synthetic plugs (polyethylene or polypropylene) are preferred when processing organic solvents or any media that might wick through a cotton plug.
<h2 dir="ltr"> Compare Serological Pipette Volumes </h2>
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<ul>
<li>1 mL &ndash; Red ISO 7550 colour, 1-piece construction, 0.01 mL graduations; used for small-volume media transfer.</li>
<li>2 mL &ndash; Yellow ISO 7550 colour, 1-piece construction, 0.1 mL graduations; used for buffer preparation.</li>
<li>5 mL &ndash; Blue ISO 7550 colour, 1-piece construction, 0.1 mL graduations; used for media preparation and dilutions.</li>
<li>10 mL &ndash; Orange ISO 7550 colour, 3-piece construction, 0.1 mL graduations; used for flask media changes.</li>
<li>25 mL &ndash; Green ISO 7550 colour, 3-piece construction, 0.5 mL graduations; used for bulk media dispensing.</li>
<li>50 mL &ndash; Purple ISO 7550 colour, 3-piece construction, 1.0 mL graduations; used for large flask media changes.</li>
</ul>
<h2 dir="ltr"> Specifications Context</h2>
 Serological pipettes are manufactured from optically clear virgin polystyrene in Class 100,000 cleanroom conditions. Sterility: individually wrapped pipettes are EO-sterilised to SAL 10⁻⁶; non-pyrogenic, non-cytotoxic, DNase-free, and RNase-free per lot. ISO 7550 defines colour coding for serological pipettes; many US suppliers follow this standard, though it is not universally mandated. Calibrated to deliver (TD) with blow-out (marked with a double band or 'TD' marking) &mdash; all liquid must be expelled from the tip for accurate delivery. Globe Scientific, Nest Scientific, and Falcon remain the dominant serological pipette brands in North American cell culture labs.&nbsp; 
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Improve consistency and reduce handling variation with reliable serological pipette solutions. Request a quote from <a href="https://mbpinc.net/contact-us">the expert team at Molecular Biology Products Inc. (MBP).</a>

Serological Pipettes

SPL Serological Pipettes

SPL serological pipettes — color-coded, individually wrapped, sterile to SAL 10⁻⁶. 1 mL Yellow (91001), 2 mL Green (91002), 5 mL Blue (91005), 10 mL Orange (91010), 25 mL Red (91025), 50 mL Purple (04-2149). For cell culture and sterile liquid transfer.

Serological Pipette 1ml, Yellow, Individual, Sterile to SAL10-6. Individually wrapped, 200 Bags/Pack, 4 Packs/Case

Serological Pipette 2ml, Green, Individual, Sterile to SAL10-6. Individually wrapped, 150 Bags/Pack, 4 Packs/Case

Serological Pipette 5ml, Blue, Individual, Sterile to SAL10-6. Individually wrapped, 100 Bags/Pack, 4 Packs/Case

Serological Pipette 10ml, Orange, Individual, Sterile to SAL10-6. Individually wrapped, 100 Bags/Pack, 4 Packs/Case

Serological Pipette 25ml, Red, Individual, Sterile to SAL10-6. Individually wrapped, 50 Bags/Pack, 4 Packs/Case

Serological Pipette 50ml, Purple, Individual, Sterile to SAL10-6. Individually wrapped, 40 Bags/Pack, 4 Packs/Case

<h2 dir="ltr">What are Cell Culture Inserts?</h2>
 Cell culture inserts (also called Transwell inserts or permeable supports) are sterile, single-use polystyrene well inserts with a permeable membrane floor that suspend cells above the bottom of a standard multi-well plate, creating separate apical (upper, inside insert) and basolateral (lower, outside insert in the well below) compartments. The membrane allows diffusion of media, nutrients, metabolites, and signaling molecules between the two compartments while physically separating the two cell populations.&nbsp;Choose PET membranes for imaging and clear-bottom visualization; choose PC membranes for superior cell attachment; choose pore size based on whether cells need to migrate through the membrane (&ge;3 &micro;m) or be retained on it (0.4 &micro;m and 1 &micro;m).
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="color: #000000;" role="presentation">6 Inserts / 6-Well Plate for Tissue Engineering - Ideal for skin models and complex cell differentiation where a larger surface area is required.</li>
<li dir="ltr" style="color: #000000;" role="presentation">12 Inserts / 12-Well Plate for Transport Assays - Balanced format for nutrient diffusion studies and metabolic monitoring.</li>
<li dir="ltr" style="color: #000000;" role="presentation">12 Inserts / 24-Well Plate for&nbsp;High Throughput Screening - Designed for migration and chemotaxis assays with efficient reagent use.</li>
<li style="color: #000000;">SPLInsert&trade; Hanging formats with transparent, sterile PC or PET membranes.</li>
<li style="color: #000000;">SPLInsert&trade; Standing format for cell culture insert applications.</li>
<li style="color: #000000;">100 mm cell culture insert dishes with 3.0 &mu;m PC membranes, translucent construction, tissue-culture treatment, and sterile packaging.</li>
</ul>
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<h2 dir="ltr">How to Choose Cell Culture Inserts</h2>
 PET vs. PC Membrane PET (polyethylene terephthalate) membranes are optically transparent, allowing direct visualization of cells growing on the membrane by standard phase-contrast and fluorescence microscopy without removing the insert. PET is the first choice when imaging cells on the membrane is part of the experimental readout (tight-junction immunofluorescence, GFP localization, wound-healing on the membrane). PC (polycarbonate) membranes provide superior cell attachment due to their higher surface energy &mdash; often used for primary epithelial, endothelial, and intestinal cells that are difficult to culture on PET. PC membranes are opaque, blocking transmitted light microscopy through the membrane. Pore Size by Application 0.4 &micro;m pore inserts prevent cell migration and cell-cell contact between apical and basolateral compartments while allowing free diffusion of molecules &lt;0.4 &micro;m (nutrients, cytokines, small molecules, metabolites). Standard for epithelial barrier function assays (TEER measurement, permeability assays), secretion studies (measuring cytokine secretion from the basolateral side), and any co-culture where cell-cell contact must be prevented. 1 &micro;m pore inserts similarly prevent cell migration but allow slightly larger molecule diffusion &mdash; used for exosome transport studies. 3 &micro;m pore inserts allow poorly migratory cells (some endothelial, fibroblast lines) to migrate through. 8 &micro;m pore inserts support tumor cell migration, invasion, chemotaxis, and hematotaxis assays &mdash; the standard pore size for Boyden chamber&ndash;style migration and invasion assays. Plate Format and Insert Configuration NEST Scientific inserts are pre-packed in multi-well plates: 6-well plate format (6 inserts per plate), 12-well plate format (12 inserts per plate), 24-well plate format (12 inserts per plate). Inserts are centered in wells by their flange geometry, preventing the membrane from touching the well sides and creating a wicking path. The apical-basolateral gap (distance from membrane floor to well bottom) is designed to allow media access to the basolateral face and pipette access to the lower chamber for sampling. TC-Treated vs. Non-Treated Membrane TC-treated membrane inserts promote adherent mammalian cell monolayer formation on the insert membrane. Non-treated membrane inserts (some PC formats) are used for migration and invasion assays where cells in the apical chamber must actively migrate through the membrane pore to reach the basolateral side &mdash; a non-treated surface reduces passive cell adhesion that would produce false-positive migration signals. Transferability NEST Scientific cell culture inserts can be transferred from one plate to a fresh plate for media changes, cumulative secretion studies, and time-course experiments &mdash; a key advantage over the non-insert well format. Transfer the insert using sterile forceps or the insert flange. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific cell culture inserts are manufactured from polystyrene with PET or PC membranes in TC-treated (vacuum plasma, contact angle &lt;10&deg;) or non-treated formats. Inserts are packed in individually sealed, peelable Tyvek film and blister packaging for sterility assurance &mdash; one plate per pack, sterile. E-beam sterilized to SAL 10⁻⁶; DNase/RNase-free and non-pyrogenic per lot. The insert flange geometry is designed to center the insert in the well, preventing membrane-wall contact and wicking. NEST Scientific cell culture inserts in PET and PC membranes across the four pore sizes (0.4, 1, 3, 8 &micro;m) and three plate formats (6-, 12-, 24-well) are available from MBP. 
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Bridge the gap between routine culture and physiological discovery. Optimize your laboratory procurement with&nbsp;Molecular Biology Products Inc. <a href="https://mbpinc.net/contact-us">Connect with our specialists</a> for a customized quote today.

Cell Culture Inserts

SPLInsert Hangings

SPLInsert™ Hanging, PC Membrane, Transparent, Sterile

SPLInsert™ Hanging Inserts with polycarbonate (PC) membrane by SPL Life Sciences are sterile, rim-supported transwell inserts in 6-well, 12-well, and 24-well formats at 3 µm or 8 µm pore sizes. Growth areas 0.33–4.52 cm²; membrane thickness 10–23 µm. Six SKUs (35106–35224). For cell migration, co-culture, and barrier function assays in research and biopharma labs.

SPLInsert™ Hanging, 6 Inserts/6 well plate, PC, 3μm, Growth Area 4.52㎠, 1.5~2.5ml, Translucent, Sterile, 24/Case

SPLInsert™ Hanging, 6 Inserts/6 well plate, PC, 8μm, Growth Area 4.52㎠, 1.5~2.5ml, Transparent, Sterile, 24/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PC, 3.0μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Translucent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PC, 8.0μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Translucent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PC, 3μm, Growth Area 0.33㎠, 0.2~0.35ml, Translucent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PC, 8μm, Growth Area 0.33㎠, 0.2~0.35ml,  Translucent, Sterile, 48/Case

SPLInsert™ Hanging, PET Membrane, Transparent, Sterile

SPLInsert™ Hanging PET Membrane Inserts (37006 series) by SPL: transparent PS frame, PET membrane, low protein binding, sterile. Available for 6-well (4.52 cm²), 12-well (0.90 cm²), and 24-well (0.33 cm²) plates in 0.4, 3, and 8 µm pore sizes. 24–48 inserts/case.

SPLInsert™ Hanging, 6 Inserts/6 well plate, PET, 0.4μm, Growth Area 4.52㎠, 1.5~2.5ml, Transparent, Sterile, 24/Case

SPLInsert™ Hanging, 6 Inserts/6 well plate, PET, 3μm, Growth Area 4.52㎠, 1.5~2.5ml, Transparent, Sterile, 24/Case

SPLInsert™ Hanging, 6 Inserts/6 well plate, PET, 8μm, Growth Area 4.52㎠, 1.5~2.5ml, Transparent, Sterile, 24/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PET, 0.4μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PET, 3.0μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/12 well plate, PET, 8.0μm, Membrane Diameter 10.50mm, Growth Area 0.90㎠, 0.40~1.00ml, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PET, 0.4μm, Growth Area 0.33㎠, 0.2~0.35m, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PET, 3μm, Growth Area 0.33㎠, 0.2~0.35ml, Transparent, Sterile, 48/Case

SPLInsert™ Hanging, 12 Inserts/24 well plate, PET, 8μm, Growth Area 0.33㎠, 0.2~0.35ml, Transparent, Sterile, 48/Case

SPLInsert Standing

SPLInsert™ Standing

SPLInsert Standing transwell inserts (SPL Life Sciences): PC or PET track-etched membrane, 0.4–8µm pore, 6-well (4.06cm²) or 24-well (0.60cm²) format, sterile, DNase/RNase-free. 8 variants, 6 or 12 inserts per plate.

SPLInsert™ Standing, 6 Inserts/6 well plate, PC, 8μm, Growth Area 4.06㎠, 2~6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 12 Inserts/24 well plate, PC, 8μm, Growth Area 0.60㎠, 0.2~0.6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 6 Inserts/6 well plate, PET, 0.4μm, Growth Area 4.06㎠, 2~6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 6 Inserts/6 well plate, PET, 3μm, Growth Area 4.06㎠, 2~6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 6 Inserts/6 well plate, PET, 8μm, Growth Area 4.06㎠, 2~6ml, Transparent, Sterile, 24/Case

SPLInsert™ Standing, 12 Inserts/24 well plate, PET, 0.4μm, Growth Area 0.60㎠, 0.2~0.6m, Transparent, Sterile, 48/Case

SPLInsert™ Standing, 12 Inserts/24 well plate, PET, 3μm, Growth Area 0.60㎠, 0.2~0.6ml, Transparent, Sterile, 48/Case

SPLInsert™ Standing, 12 Inserts/24 well plate, PET, 8μm, Growth Area 0.60㎠, 0.2~0.6ml, Transparent, Sterile, 48/Case

BioFactory Accessories

<h2 dir="ltr"> What are Accessories for NEST BioFactory&trade; Systems?</h2>
 NEST BioFactory&trade; accessories are the replacement caps, silicone tubing, vent filters, overcaps, and pre-assembled closed-system cap assemblies used to configure BioFactory units for closed aseptic liquid transfer, gas exchange management, and multi-unit serial connections in cGMP and research-scale bioprocess workflows. The accessory set enables the BioFactory narrow-mouth cap to function as a closed-system liquid transfer port &mdash; media, wash buffer, trypsin, and cell harvest supernatant are transferred in and out through the silicone tubing connection without opening the vessel. Accessories for the BioFactory are required when operating the BioFactory as a closed system; for simple open-pour workflows using wide-mouth caps, standard vent and plug seal caps are the only accessories needed.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
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BioFactory media transfer caps
</li>
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Sterile tubing and connector sets
</li>
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Fluid transfer adapters for cell culture vessels
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
BioFactory bottle caps and closures
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Sterile quick-connect systems
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Liquid handling accessories
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Vessel handling and support tools
</li>
<li dir="ltr" style="color: #000000;" aria-level="1">
Closed-system connectors for aseptic processing
</li>
</ul>
<h2 dir="ltr"> Key BioFactory Accessory Categories</h2>
 BioFactory Cap with Silicone Tube (CPC or Luer Connector) The BioFactory cap with silicone tube is the primary closed-transfer accessory: an adaptor cap that fits the narrow-mouth port of the BioFactory unit, pre-assembled with 80 cm of silicone tubing and a terminal connector &mdash; either a male CPC (clamped pressure connector / quick-connect) or a standard Luer lock &mdash; for coupling to peristaltic pumps, other BioFactory units, centrifuge tubes, or PETG media bottle assemblies. Two tubing I.D. &times; O.D. configurations are available: 1/4" I.D. &times; 3/8" O.D. and 3/8" I.D. &times; 5/8" O.D., covering standard peristaltic pump head bore sizes. The female sealing cap included with each assembly caps the connector between uses, maintaining sterility. Overcaps: Vented and Solid Overcaps are secondary dust and contamination barriers placed directly on top of the BioFactory adaptor caps during culture incubation. The vented overcap contains a 0.22 &micro;m hydrophobic breathable membrane, allowing gas exchange through the cap stack while blocking particles and microbial contamination &mdash; used during active shaker or orbital incubation when the unit's primary cap is an adaptor cap rather than a vented cap. The solid overcap provides an additional dust and contamination seal with no gas exchange &mdash; used for short-term storage or transport outside the incubator. SPT-50 Silicone Hose SPT-50 silicone hose (3/8" I.D. &times; 5/8" O.D., 50 inches / 15 meters) is the bulk tubing used to connect multiple BioFactory units in series, connect BioFactory ports to peristaltic pump heads, or extend the reach of the transfer cap assembly. SPT-50 tubing is manufactured from silicone, is individually wrapped and sterile, and passes dissolution testing per BPOG (BioPhorum Operations Group) criteria per NEST product documentation, supporting its use in biopharmaceutical production environments. Premium Accessory Package NEST offers a BioFactory Accessory Premium Package that includes 2 different adaptor caps, 1 hose clamp, 1 adaptor connector, 1 vent filter (50 mm), 1 additional hose clamp, and 15 m of SPT-50 silicone hose &mdash; all individually wrapped and sterile in a single kit. This package provides all components needed to set up a closed-system BioFactory media management workflow from a single kit, reducing the number of individual part orders required. Vent Filters for Gas Exchange Management For BioFactory units using adaptor caps rather than standard vented caps, 50 mm inline vent filters (0.22 &micro;m hydrophobic PTFE membrane) are installed on the air inlet/outlet tubing line to maintain sterile gas exchange during media addition, harvest, and incubation. The vent filter is rated for high flow rates without creating excessive back-pressure that would resist liquid movement through the system. Compatibility: Narrow-Mouth BioFactory Units Only All BioFactory accessory caps and tubing assemblies are designed exclusively for the narrow-mouth BioFactory configuration. Wide-mouth BioFactory units use standard wide-mouth vent and plug seal caps only; they are not compatible with accessory cap assemblies. Confirm that your BioFactory units have narrow-mouth ports before ordering the accessory cap set. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST BioFactory&trade; accessory silicone tubing and cap assemblies are manufactured from USP Class VI silicone (TPE/silicone) and polycarbonate or PP cap bodies, individually wrapped and sterile. All components have passed dissolution testing per BPOG dissolution test criteria conducted by a certified third-party laboratory per NEST Scientific documentation. The standard Luer head connections conform to ISO 594 / GB/T 1962 for reliable compatibility with standard laboratory fittings. Vent filters use a 0.22 &micro;m hydrophobic PTFE membrane; overcaps use a 0.22 &micro;m hydrophobic membrane. NEST BioFactory accessories are carried by MBP alongside BioFactory units. 
&nbsp;
Need the right BioFactory setup? <a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact MBP today</a> for technical assistance.

Biofactory System Accessories

Product Literature

SPT-50 Hose，Inner dia# 3/8 Inches(9.5 mm)，Out dia# 5/8 Inches(15.9 mm), 50 Inches/ 15 meters, Individually Wrapped, Sterile, 1/pk, 1/cs

744001 is the SPT-50 platinum-cured silicone hose: inner diameter 3/8 inch (9.5 mm), outer diameter 5/8 inch (15.9 mm), 15 meters length. 50 Shore A durometer, USP Class VI compliant, European Pharmacopoeia 3.1.9 certified. Sterile, individually wrapped. Suitable for peristaltic pump fluid transfer, sterile-fill manufacturing, and GMP bioprocess lines. 1/pk, 1/cs.

Manufacturer

<h2 dir="ltr">What are Media Storage Bottles?</h2>
 Media storage bottles for cell culture are sterile, single-use or multi-use plastic containers used to store and dispense tissue culture media, sera, buffers, and other aqueous solutions in research and biopharmaceutical laboratory settings. Choose PC bottles for autoclavable reuse; PET for single-use media storage and transport; PETG for low-temperature archival, closed-system transfer, and pH-sensitive long-term storage.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/petg-square-bottles">PETG Square Bottles </a>for space-saving laboratory storage
</li>
<li dir="ltr" aria-level="1">
<a href="consumables/cell-culture/media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;">PETG Bottle With</a><a href="media-storage-bottles/petg-bottle-with-accessories" style="color: #236fa1;"> Accessories&nbsp;</a><a href="https://mbpinc.net/petg-bottle-with-accessories" title="PETG Bottle With Accessories" style="color: #236fa1; text-decoration: underline;"></a>for specialized fluid handling
</li>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/pc-media-bottles">PET Media Bottles </a>for high-clarity reagent containment
</li>
<li dir="ltr" aria-level="1">
<a href="media-storage-bottles/pc-media-bottles">PC Media Bottles </a>for superior mechanical durability
</li>
</ul>
<h2 dir="ltr"> How to Choose Media Storage Bottles</h2>
 Material: PC vs. PET vs. PETG PC (polycarbonate) bottles are window-clear, shatter-proof, and rated for repeated autoclaving (up to 5&ndash;10 cycles) &mdash; the standard for labs that prepare and sterilize media in-house. PC is resistant to alcohols, weak acids, and aliphatic hydrocarbons; it is not compatible with strong bases or organic solvents. PC contains BPA (bisphenol A) by its monomer chemistry and is not recommended for long-term hormone-sensitive cell culture applications. PET bottles are optically clear, lighter than glass, non-cytotoxic, and non-autoclavable, rated from &minus;60 &deg;C to +60 &deg;C. They are the cost-effective standard for single-use media and sera storage in non-autoclave workflows. PETG (glycol-modified PET) adds a glycol co-monomer that reduces crystallinity, improving impact resistance, low-temperature stability to &minus;80 &deg;C, and flexibility. PETG is the preferred material for long-term storage of pH-sensitive liquids, protein/vaccine intermediates, and applications requiring closed-system accessory assemblies. Round vs. Square Bottles Round bottles (Boston round, GL45 thread) are compatible with standard bottle-top vacuum filtration systems (Millipore Stericup, Corning Costar) and are the format of choice for media vacuum-filtered in-house. Square bottles conserve refrigerator and cold room shelf space due to their flat sides and stackable design, reducing storage footprint by approximately 25% versus equivalent-volume round bottles. NEST Scientific PET and PETG square media bottles feature molded graduations and a white labeling area on two sides. Volume Range Standard media bottle volumes are 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL, corresponding to common media and sera kit sizes. A 250 mL bottle accommodates a standard 250 mL media aliquot; a 500 mL bottle holds standard FBS lots or 500 mL media packs. Confirm that the bottle's nominal volume includes 15% additional headspace for mixing, thawing expansion, and transfer operations. Accessory Assembly vs. Standard Cap Standard PETG square media bottles ship with an HDPE screw cap &mdash; suitable for storage and manual transfer. PETG bottles with accessory caps (bi-directional transfer cap + TPE tubing + Luer lock or CPC connector, NEST catalog 3525xx series) create a fully closed aseptic liquid transfer system &mdash; used in cGMP environments, bioprocess research, and any workflow where open-pour media transfer creates contamination or regulatory risk. Sterility and Certifications For cell culture use, all media storage bottles must be certified DNase/RNase-free, non-pyrogenic (endotoxin-free), and non-cytotoxic. NEST Scientific PET and PETG media bottles meet USP Class VI and ISO 10993 biocompatibility requirements, are manufactured in ISO 8 cleanrooms, and are gamma or E-beam-sterilized. PC bottles from autoclave-capable suppliers use virgin Lexan&reg; PC resin compliant with USP Class VI.
&nbsp;
<h2 dir="ltr">Specifications Context</h2>
 NEST Scientific square PET media bottles (catalog 334xxx) and PETG media bottles (catalog 352xxx) are manufactured from 100% virgin material in ISO 8 cleanrooms. Both materials meet USP Class VI and ISO 10993 biocompatibility requirements. Bottles are gamma irradiated in the USA or E-beam sterilized to SAL 10⁻⁶; each lot is certified DNase/RNase-free, pyrogen-free, and endotoxin-free. PETG bottles feature molded graduations (not printed), smooth inner surface to minimize residue on the wall, a one-shot-molded cap seat (no inner gasket required), and a double-bagged sterile packaging format meeting GMP purification workshop material-entry requirements. NEST Scientific PET and PETG media bottles are stocked by MBP in sizes from 60 mL to 1,000 mL. 
Don't wait for a contamination crisis to upgrade your storage. <a href="https://mbpinc.net/contact-us" title="Reach out to the Molecular Biology Products Inc.">Reach out to the Molecular Biology Products Inc.</a> experts for a quote and keep your reagents locked down.

Media Storage Bottles

<h2 dir="ltr">What are PET Media Bottles?</h2>
 PET (polyethylene terephthalate) media storage bottles are single-use, sterile, optically clear plastic containers for storing tissue culture media, fetal bovine serum, PBS, HBSS, trypsin-EDTA, and other aqueous laboratory solutions. PET is manufactured by an injection stretch-blow molding (ISBM) process that produces glass-like optical clarity, low gas permeability (especially to CO₂ and O₂ compared to polystyrene), excellent mechanical strength, and a smooth inner surface that minimizes liquid residue. PET bottles are BPA-free, non-autoclavable, and rated for use from &minus;60 &deg;C to +60 &deg;C.
&nbsp;They are the dominant single-use format for media and sera storage in cell culture because of their glass-comparable clarity, low extractable profile, and cost-effectiveness compared to borosilicate glass. Choose PET media bottles as the default single-use format for ready-to-use media and sera storage, cold room archiving, and any application where autoclaving is not required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
60mL PET Media Bottles for small-scale sampling
</li>
<li dir="ltr" aria-level="1">
125mL Sterile PET Bottles for routine reagent handling
</li>
<li dir="ltr" aria-level="1">
250mL PET Storage Bottles for mid-range media volumes
</li>
<li dir="ltr" aria-level="1">
500mL/0.5L PET Bottles for standard laboratory preparations
</li>
<li dir="ltr" aria-level="1">
1000mL/1L PET Media Bottles for large-batch stock solutions
</li>
<li dir="ltr" aria-level="1">
2L High-Volume PET Bottles for industrial-scale storage
</li>
<li dir="ltr" aria-level="1">
5L PET Media Vessels for bulk liquid containment
</li>
<li dir="ltr" aria-level="1">
Double-layer Packaged Bottles for enhanced cleanroom safety
</li>
<li dir="ltr" aria-level="1">
Square Shoulder Tray-Packed Bottles for secure, organized transport
</li>
</ul>
<h2 dir="ltr"> How to Choose PET Media Bottles</h2>
 Round vs. Square Format Round PET media bottles (Boston round, GL45) are directly compatible with bottle-top vacuum filtration systems and standard lab bottle accessories. Square PET media bottles (NEST Scientific catalog 334xxx) feature flat sides that stack efficiently in refrigerators and cold rooms, reducing footprint by approximately 25% versus round bottles and preventing rolling when stored horizontally. NEST PET square bottles have molded graduations (not printed), a white labeling area on two sides, and embossed markings for durable identification. Volume Selection PET media bottles are available in 30 mL, 60 mL, 125 mL, 250 mL, 500 mL, and 1,000 mL volumes. A 500 mL bottle accommodates a standard 500 mL aliquot of cell culture media (DMEM, RPMI, MEM) or a standard FBS lot aliquot. The 1,000 mL bottle holds a full 1 L media preparation. Each bottle includes approximately 15% additional headspace above the nominal volume for thawing expansion (&minus;20 &deg;C frozen FBS thaw) and transfer convenience. Cap Material NEST Scientific PET media bottle caps are made from HDPE (high-density polyethylene), free of heavy metals per regulatory testing. The cap is designed through a one-shot mold process without an inner gasket, fitting securely onto the bottle neck with enhanced sealing performance &mdash; an important feature for leak-proof horizontal storage and air transport. Gas Barrier Properties PET and PETG both reduce gas permeability to CO₂ and O₂ compared to polystyrene, maintaining medium pH and preventing dissolved gas equilibration during storage. For pH-sensitive media containing bicarbonate buffers, PET and PETG are materially superior to polystyrene bottles, which allow CO₂ outgassing and pH shift during storage at room temperature or 4 &deg;C. Sterility and Lot Traceability NEST Scientific PET media bottles are gamma irradiated or E-beam sterilized; each lot is certified DNase/RNase-free, human DNA-free, pyrogen-free, and non-cytotoxic per lot certificate. Lot numbers are printed on each bottle and packaging for traceability. Shelf life is 3 years from the production month per NEST lot release data. PET bottles meet FDA requirements for materials used in contact with sterile liquid pharmaceuticals. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific PET square media bottles are manufactured from 100% virgin PET resin via the ISBM process in ISO 8 cleanrooms. Material meets USP Class VI and ISO 10993 biocompatibility, FDA requirements, and USP &lt;661&gt; container testing for plastics in contact with sterile solutions. Operating temperature: &minus;60 &deg;C to +60 &deg;C; not autoclavable. Smooth inner wall minimizes residue; molded graduations on two sides. The HDPE cap is heavy-metal-free. Packaging is double-bagged in sterile format for GMP-compatible cleanroom entry. Shelf life: 3 years from production month. As of 2026, NEST Scientific PET media bottles are stocked by MBP in the full size range. 
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Don&rsquo;t wait for a compromised sample to upgrade your containment. <a href="https://mbpinc.net/contact-us">Contact now </a>and request a quote from Molecular Biology Products Inc. and keep your lab results (and your PI) stress-free.

PET Media Bottles

SQUARE CULTURE MEDIUM BOTTLE, PET, STERILE

Biologix Square Culture Medium Bottles PET Sterile — sterile square PET bottles for cell culture media storage and dispensing. Two variants: 06-1000 (1000 ml) and 06-1500 (500 ml); 24 bottles/bag, 2 bags/case. Available from MBP.

SQUARE CULTURE MEDIUM BOTTLE, 1000ML, PET, STERILE; 24 BOTTLES/BAG, 2 BAGS/CASE

SQUARE CULTURE MEDIUM BOTTLE, 500ML, PET, STERILE; 24 BOTTLES/BAG, 2 BAGS/CASE

<h2>What Are Laboratory Heaters?</h2>
&nbsp;
Laboratory heaters are temperature-control instruments that raise the temperature of a fluid (deionized water, buffer, glycol, or silicone oil) and maintain it at a stable setpoint for connected equipment or for samples immersed in the bath. Open bath heaters expose the fluid surface and allow direct sample contact; closed-system heaters circulate heated fluid through external tubing to jacketed vessels, columns, or instruments without evaporation loss. Immersion circulators clip onto an existing open bath and add a heating element and pump without requiring a dedicated bath unit. Applications span reagent and media warming, enzyme activity assays, blood banking thaw protocols, hybridization reactions, and food stability testing. Choose an open bath heater for direct sample immersion at up to 100 degrees C; choose a closed-system heater for external circulation, jacketed vessels, or temperatures above 100 degrees C.
&nbsp;
<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Julabo Heating Circulators use stainless steel bath tanks and powerful pumps to provide uniform warmth for high-temperature research.
</li>
<li dir="ltr" aria-level="1">
Refrigerated-Heated Circulators offer versatile temperature ranges, allowing you to switch between deep cooling and intense heating in one system.
</li>
<li dir="ltr" aria-level="1">
Ai Compact Heated Recirculators deliver reliable heating up to 100&deg;C in a space-saving design perfect for small-scale benchtop projects.
</li>
<li dir="ltr" aria-level="1">
Induction Heaters with Timers provide rapid, localized heating for specialized material science using advanced frequency technology.
</li>
<li dir="ltr" aria-level="1">
HUBER Heating Units and Fluids combine user-friendly digital interfaces with high-quality transfer media for smooth, long-term thermal management.
</li>
<li dir="ltr" aria-level="1">
PolyScience Heated Circulators feature robust safety controls and digital displays for precise management of high-temperature laboratory runs.
</li>
</ul>
&nbsp;
<h2>How to Choose a Lab Heater</h2>
&nbsp;
Heating method: open bath vs. closed system vs. immersion
Open bath heaters (sometimes called circulating water baths) are the most common format for biological and enzymatic work up to 100 degrees C. They allow direct tube or flask immersion and are easy to clean. Closed-system heaters use a smaller reservoir, a stronger pump, and a powerful heating element to push heated fluid externally, preferred for jacketed vessels, columns, or high-temperature applications. Immersion circulators convert an existing open bath into a constant-temperature bath at a lower cost than a full benchtop circulator.
Temperature range
Most open bath heaters cover ambient temperatures to 99 degrees C, which covers cell culture, hybridization, and most enzymatic workflows. Closed-system heaters reach 150 degrees C to 200 degrees C for materials testing, food stability, and polymer research. Certain high-temperature models reach 250 degrees C and above, but require compatible heat-transfer fluids such as silicone oil rather than water.
Bath volume and heating capacity
Match bath volume to your sample load. Small-footprint open baths start at 2 L to 4 L; larger models run to 28 L or more for high-throughput workflows. Heating capacity in kW determines warm-up time: 1 kW to 2 kW is typical for standard benchtop units, while closed-system heaters with external circulation often specify 3 kW or higher for rapid heat-up.
Temperature uniformity and stability
Circulating heaters outperform non-circulating models on temperature uniformity across the bath. Digital PID controllers maintain stability to plus or minus 0.1 degrees C at most biological setpoints. Open baths without circulation are acceptable for batch incubations but not for assays requiring tight temporal control.
Control and connectivity
Entry-level units offer a simple digital setpoint; advanced models include programmable temperature profiles (ramp and soak), over-temperature alarms, lockout features, RS-232/RS-485 connectivity, and compliance with IEC 61010-1 and DIN 12876.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Temperature stability specifications are measured at a steady-state setpoint with the lid closed; actual stability in use depends on room temperature fluctuations, lid openings, and sample volume. Rated working temperature in DIN 12876-1 Class I and Class II units must be at least 25 degrees C below the bath fluid flash point, relevant if ethanol or glycol solutions are used as bath fluid. For biological applications using water as the bath fluid, this constraint is not relevant. Units listing IEC 61010-2-010 certification meet safety requirements for laboratory heating equipment.&nbsp;
&nbsp;
Looking to turn up the precision in your lab? Explore our heaters and <a href="https://mbpinc.net/contact-us">reach out to the friendly MBP team</a> for a custom quote today.

Heaters

Recirculating Heater Specifications

Ai H200-15 200°C 15L heated recirculator

250°C rated flexible insulated stainless steel tubing (40" L), pipe connection with M16 connection fitting

1/2" NPT to 3/8" hose barb drain port adapter

H200-7sst.220 Standard Package

H200-15sst.220 Standard Package

SST Compact Desktop Heated Recirculator 220V

Ai 200°C 304 stainless steel desktop heated recirculators at 220 V, available in 7 L and 15 L capacities. Both models deliver 2800 W, ±2°C stability, 16-ft pump head, and UL+CSA certification.

Ai 200Â°C 7L SST Compact Desktop Heated Recirculator 220V

Ai 200Â°C 15L SST Compact Desktop Heated Recirculator 220V

SST Compact Heated Recirculator 220V Specifications

H100-7sst.220 Standard Package

Ai H100-15 100°C 15L heated recirculator

H100-15sst.220 Standard Package

SST Compact Heated Recirculator 220V

Ai SST Compact Heated Recirculator 220V by Across International. H100-7sst.220: 7L, 1,600W, 10.5×17×17". H100-15sst.220: 15L, 2,600W, 12.5×17×19.25". Both: 100°C max, 20 L/min adjustable pump, 304 SST reservoir, 220V, UL 61010-1 / CSA C22.2 certified.

Ai 100Â°C 7L Capacity SST Compact Heated Recirculator 220V

Ai 100Â°C 15L Capacity SST Compact Heated Recirculator 220V

Freezers.Accessories

<h2>What Are Laboratory Freezer Accessories?</h2>
&nbsp;
Laboratory freezer accessories fall into four functional groups: organizational hardware that maximizes storage density and sample accessibility; temperature monitoring and alarming systems that protect sample integrity and support compliance; backup protection systems that guard against temperature excursions during power failures; and containment and labeling consumables that prevent sample misidentification and physical damage. Effective freezer accessory selection increases the number of samples stored per unit volume, reduces the time the freezer door is held open during access (directly protecting sample stability), and provides the electronic audit trail required by regulatory frameworks, including CAP, CLIA, GxP, and FDA 21 CFR Part 11. Match accessories to the freezer temperature, sample tube format, and regulatory compliance framework applicable to your laboratory.
&nbsp;
<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Storage Boxes &amp; SST Drawers: High-quality stainless steel drawer sets and storage boxes specifically designed to maximize space and organization within upright and stackable ULT freezers.
</li>
<li dir="ltr" aria-level="1">
CO2 Backup Systems: Critical emergency backup solutions for ultra-low freezers that safeguard your inventory against sample loss during power outages or mechanical failures.
</li>
<li dir="ltr" aria-level="1">
Insulated Shipping Cubes: Advanced VIP insulated containers engineered for the secure and temperature-stable transport of high-value biological materials.
</li>
<li dir="ltr" aria-level="1">
Specialized Inventory Tools: Custom-fit shelving and racking solutions that ensure unrestricted airflow and easy access to your most important specimens.
</li>
</ul>
&nbsp;
<h2>How to Choose Freezer Accessories</h2>
&nbsp;
Freezer racks and storage boxes
Standardized upright freezer racks hold stacked sample storage boxes in fixed positions within the cabinet, enabling systematic sample organization and reducing search time during access. Standard 2-inch (5 cm) sample boxes are the most common format and fit racks in most upright and chest ULT freezers; 3-inch (7.5 cm) boxes store fewer boxes per rack but hold more tubes per box. Rack configurations include fixed upright racks (anchored to cabinet floor or wire shelf), side-access sliding drawer racks (pulled out horizontally for access without removing the whole rack), and adjustable racks for non-standard box sizes. Confirm rack dimensions against the freezer cabinet's internal width, depth, and shelf spacing before ordering.
Sample storage boxes and dividers
Cryogenic sample storage boxes (cryoboxes) are fabricated from cardboard (economy, single-use), high-density polyethylene (HDPE, reusable), polypropylene (PP, autoclavable), or stainless steel (autoclavable, chemically resistant) and hold 9x9 (81 positions), 10x10 (100 positions), or 8x8 (64 positions) tubes per box. Insert dividers (indexed alphanumerically) to create individual tube compartments to prevent tube movement, mixing, and label abrasion during access. For 1.5 mL microcentrifuge tubes, 81-position 9x9 dividers in a 2-inch box are standard; for 15 mL conical tubes, dedicated large-tube racks are required.
Temperature monitoring and data logging
Continuous temperature monitoring systems for ULT freezers include: standalone digital thermometers with external min/max recording probes inserted through door gasket access ports; USB data loggers that record temperature at programmable intervals (1-60 minutes) with NIST-traceable calibration certificates; Wi-Fi-connected wireless sensors with 24/7 real-time cloud monitoring, configurable high/low alarm thresholds, and email/SMS notification (rated to -100 degrees C for ULT applications); and integrated freezer-connected monitoring systems that read data directly from factory-installed cabinet sensors via a dedicated data port. Annual calibration of monitoring probes against NIST-traceable standards is required for CAP, CLIA, and GxP compliance.
Backup protection systems
CO2 backup injection systems activate when cabinet temperature rises above a preset high-alarm threshold (typically 10 degrees C above the normal setpoint), injecting CO2 from an external cylinder through a solenoid-controlled valve into the freezer cabinet, maintaining temperature for 4-12 hours. LN2 (liquid nitrogen) backup injection systems provide similar protection at deeper temperatures and with longer holdover time, but require access to an LN2 supply. Both systems require connection to an external gas supply, installation of solenoid valves, and periodic cylinder replacement or LN2 refill scheduling.
Cryo labels and identification
Cryogenic-rated labels are required for sample tubes stored in ULT and cryogenic conditions; standard adhesive labels lose adhesion at -80 degrees C and fall off during access in cold rooms, causing catastrophic sample misidentification. Cryogenic labels are printed on polyester or polypropylene film with acrylic or cryogenic-grade adhesive rated to -196 degrees C (liquid nitrogen temperatures). Laser-printable cryolabels, wrap-around tube labels, and flag labels are available for 0.5 mL, 1.5 mL, 2 mL, 15 mL, and 50 mL tubes. Barcode-compatible cryolabels enable integration with laboratory information management systems (LIMS).
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Standard 2-inch (5.4 cm) sample boxes fit universal rack systems across most major ULT freezer brands (Thermo Fisher TSX, PHCbi MDF-DU, Esco Lexicon); 3-inch (7.6 cm) boxes require racks specifically designed for that format. Wi-Fi temperature monitoring sensors for ULT applications should be rated for a measurement range of at least -100 degrees C to 0 degrees C; sensors not rated for ULT temperatures lose calibration accuracy below -60 degrees C. NIST-traceable temperature calibration for monitoring probes is required at least annually under CAP and CLIA accreditation, using traceable reference standards with uncertainty documentation. Cloud-based temperature monitoring systems with 21 CFR Part 11-compliant audit trails and automated compliance reporting have become standard in GxP-regulated laboratory environments, replacing manual temperature logbooks and standalone chart recorders.&nbsp;
&nbsp;
Enhance your laboratory storage setup today&mdash;explore our freezer accessories and <a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> for a quote.

Freezer Accessories

SST Storage Drawers for Ai  RapidChill -86°C Stackable Ultra low Freezer

The DrawerSet-G04h by Across International (Ai) is a stainless-steel storage drawer set rated for -86°C continuous operation, designed for the Ai RapidChill G04h ULT freezer. The set includes 4 drawer units (20 total drawers) with capacity for 60 × 2-inch vial boxes or 6,000 × 1.5 mL cryogenic vials. Vials, vial boxes, and the G04h freezer are sold separately.

Manufacture

SST Storage Drawers for Ai G04h -86C Freezers 6,000 Vials Max.

SST Storage Drawers for Ai G26h -86C Freezers 50,000 Vials Max.

SST Storage Drawers for Ai G12h -86C Freezers 21,600 Vials Max.

SST Storage Drawers for Ai G20h -86C Freezers 40,000 Vials Max.

<h2 dir="ltr">What are Vacuum Bottle-Top Filter Systems?</h2>
 Vacuum bottle-top filter systems are single-use, sterile filtration assemblies consisting of a graduated polystyrene funnel with an integrated membrane filter bonded to a polystyrene receiver bottle, connected by a hose-port adaptor to a house vacuum line or benchtop vacuum pump. The applied negative pressure (&minus;50 to &minus;100 kPa) draws liquid through the membrane into the receiver bottle, which is sealed after filtration for immediate use or cold-room storage.&nbsp;
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
50mL Vacuum Filtration Units for small-scale sterile sampling
</li>
<li dir="ltr" aria-level="1">
150mL Vacuum Filtration Systems for rapid reagent preparation
</li>
<li dir="ltr" aria-level="1">
250mL Filtration Units for routine buffer sterilization
</li>
<li dir="ltr" aria-level="1">
500mL Vacuum Filtration Cups for mid-scale media processing
</li>
<li dir="ltr" aria-level="1">
1000mL Large Volume Filtration for industrial-scale batching
</li>
<li dir="ltr" aria-level="1">
Sterile Bottle-Top Filters for secure aseptic transfer
</li>
</ul>
<h2 dir="ltr"> How to Choose Vacuum Filtration Systems</h2>
 PES vs. PVDF Membrane for Vacuum Filtration PES membranes provide low protein binding (~1&ndash;2 &micro;g/cm&sup2;), high flow rates, and broad aqueous-solution compatibility &mdash; the standard for routine DMEM, RPMI, HBSS, PBS, and supplement-containing media sterilization. PVDF membranes have slightly lower protein binding than PES and greater chemical resistance to mild organic solvents, mild acids, and alkaline solutions. Use PVDF when filtering protein-rich solutions (FBS, antibody preparations, conditioned medium with high protein content) or when mild organic solvents are present in the filter solution. Volume Selection 250 mL systems (50 mm diameter membrane, 50 mL funnel) suit small media aliquots, supplement solutions, and transfection reagent preparations. 500 mL systems (90 mm diameter membrane, 150 mL funnel) are the routine lab workhorse for standard 500 mL media bottle preparation and FBS filtration. 1,000 mL systems (90 mm diameter membrane, 350 mL funnel) handle full-liter media preparations and large-volume buffer sterilization. All NEST systems include a pre-attached, graduated polystyrene receiver bottle in the corresponding volume. Pore Size: 0.10 &micro;m vs. 0.22 &micro;m vs. 0.45 &micro;m 0.22 &micro;m is the sterilizing grade per USP &lt;1229.2&gt; and ICH Q9: retains all bacteria and fungi; used for routine cell culture media, PBS, and buffer preparation. 0.45 &micro;m is the clarification grade: used as a pre-filtration step before 0.22 &micro;m to remove large particulates from turbid or high-biomass samples (FBS, conditioned media, cell culture supernatants, crude lysates), extending 0.22 &micro;m filter throughput by 3&ndash;5&times; on difficult samples. 0.10 &micro;m is the mycoplasma-removal grade: required when preparing media for Mycoplasma-sensitive primary cell culture or when processing serum or supplementary reagents that may harbor Mycoplasma species. Note that 0.10 &micro;m filtration is substantially slower than 0.22 &micro;m due to the tighter membrane pore structure. Practical Tips for Maximum Throughput Pre-wet the membrane by briefly passing 5&ndash;10 mL of sterile water through before the sample &mdash; this wets the hydrophilic PES or PVDF membrane and reduces initial resistance. For FBS and other viscous solutions, pre-filter through 0.45 &micro;m before the 0.22 &micro;m step. Maintain a vacuum at &minus;50 to &minus;80 kPa; excessive vacuum (&gt; &minus;100 kPa) can shear fragile membrane pores and increase non-specific protein adsorption. Do not autoclave the assembled bottle-top filter &mdash; PES and PS components are not rated for autoclave sterilization. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific vacuum bottle-top filters are manufactured from polystyrene funnel and receiver bottle components with PES or PVDF membranes. PES membranes have documented low protein binding (~1&ndash;2 &micro;g/cm&sup2;) per NEST scientific USA FAQ. Membrane diameter: 250 mL volume &rarr; 50 mm; 500 mL and 1,000 mL &rarr; 90 mm. The hose connector design accommodates various standard vacuum hose diameters. NEST PES 0.22 &micro;m vacuum filters are described as comparable in performance to leading brands, including Corning Stericup, Thermo Fisher Scientific, and Sartorius, per NEST Scientific USA's product page. All NEST vacuum filters are sterile and non-pyrogenic per the lot certificate. NEST Scientific vacuum bottle-top filters are stocked by MBP in all core SKUs. 
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Because your research should move faster than a slow-dripping filter. <a href="https://mbpinc.net/contact-us" title="Contact the Molecular Biology Products Inc. team" style="color: #236fa1;">Contact the Molecular Biology Products Inc. team</a> for a quote before your deadlines catch up with you.

Vacuum Filtration

STERILE VACUUM FILTRATION UNIT, MEMBRANE: PES

Sterile Vacuum Filtration Unit 09-PES4510 with low-protein-binding PES membrane. Pre-assembled and sterile, ready for media sterilization and filtration of aqueous buffers and biological reagents. Connect to vacuum line or peristaltic pump.

Reaction Tubes

<h2 dir="ltr">What are Reaction Tubes?</h2>
 Reaction tubes are polypropylene (PP) single-use or low-retention microcentrifuge and PCR tubes used as the primary vessel for molecular biology reactions&mdash;PCR amplification, reverse transcription, restriction digestion, ligation, nucleic acid precipitation, protein extraction, and centrifugation of microvolume samples. Reaction tube quality directly affects PCR sensitivity, sample recovery, and contamination risk. Choose PCR-clean or PCR-grade tubes&mdash;certified free of DNA, DNase, RNase, and PCR inhibitors&mdash;for any molecular biology reaction where contamination would invalidate the result.
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<h2 dir="ltr">What you will find:</h2>
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<li dir="ltr" aria-level="1">Globe Scientific PS reaction tubes provide reliable compatibility with Sysmex&reg; coagulation analyzers.</li>
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Microcentrifuge reaction tubes (0.5 mL, 1.5 mL, 2.0 mL sizes)
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Conical-bottom polypropylene tubes for efficient pellet formation
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Snap-cap reaction tubes for quick, single-hand operation
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Screw-cap tubes for secure long-term sample storage
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PCR-clean and DNase/RNase-free reaction tubes
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Graduated tubes with measurement markings for accuracy
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High-temperature resistant tubes for centrifugation and incubation
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Low-retention tubes for improved sample recovery
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Sterile reaction tubes for sensitive molecular workflows
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General-purpose laboratory micro reaction vessels
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<h2 dir="ltr"> How to Choose Reaction Tubes</h2>
 Volume and Format: 0.2 mL vs. 0.5 mL vs. 1.5/2.0 mL 0.2 mL thin-wall tubes and strip tubes are the standard PCR format; consistent wall thickness (&le;0.2 mm) ensures efficient heat transfer between the thermal cycler block and the sample. 0.5 mL tubes are used for reactions generating small volumes that benefit from a snap-cap format. 1.5 mL and 2.0 mL hinged-lid tubes are the universal format for sample prep, restriction digests, ligation, protein assays, centrifugation, and short-term sample storage. Purity Grade: Standard vs. PCR-Clean vs. Certified RNase/DNase-Free Standard polypropylene tubes are adequate for general sample storage and centrifugation. PCR-clean tubes are certified free of DNA, DNase, RNase, and PCR inhibitors per lot testing &mdash; required for any PCR, RT-PCR, or sequencing preparation workflow. For RNA work, additionally confirm RNase-free certification. Eppendorf Safe-Lock Tubes (PCR clean grade) carry a two-layer resealable packaging system that maintains purity until the point of use. Low-Retention vs. Standard Polypropylene Low-retention PP is treated to reduce protein and nucleic acid adsorption to the tube wall. For reactions using &lt;50 &micro;L of a dilute nucleic acid or protein sample, low-retention tubes can recover 15&ndash;30% more material than standard PP by reducing wall-adsorption losses. Use low-retention tubes for single-cell, low-copy-number, or precious-sample workflows. Hinged Lid (Safe-Lock) vs. Snap-Cap Hinged-lid tubes (Eppendorf Safe-Lock style) have a locking latch that prevents accidental opening during centrifugation and vortexing, retaining samples at forces up to 21,300 &times; g. Standard snap-cap tubes may open at high g-forces, causing sample loss and cross-contamination. Choose Safe-Lock format for any step involving high-speed centrifugation or vigorous mixing. Color Coding Color-coded reaction tubes (natural/clear, yellow, red, blue, green) improve sample tracking at the bench and reduce the risk of mix-ups in multi-sample workflows. NEST Scientific offers colored tube collections. Confirm that the colorant does not interfere with spectrophotometric measurements (OD at 260/280 nm) if measuring nucleic acid concentration directly in the tube using a microvolume spectrophotometer. 
<h2 dir="ltr"> Specifications Context</h2>
 NEST Scientific 1.5 mL and 2.0 mL tubes are carried by MBP and provide an ISO 9001:2015&ndash;certified alternative at cost-competitive pricing for high-volume labs. Reaction tubes are available from MBP in bulk pack sizes of 500 and 1,000 tubes for 1.5 mL and 2.0 mL formats, and trays of 1,000 for 0.2 mL PCR formats. &nbsp;
For the right selection based on volume, sterility, or application type, the <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">MBP team </a>can help match the ideal reaction tube to your workflow requirements.

5530-Analyzer Consumables Catalog

5530-TDS-5530

SYSMEX: Reaction Tube, for use with Sysmex CA Series analyzers, 12000/Cs

5530 by Globe Scientific — 0.8 mL polystyrene reaction tubes (10 mm × 30 mm) for Sysmex CA50, CA500, CA1000, CA1500, and CA5000 coagulation analyzers. Sold in cases of 12,000. Available from MBP Inc. worldwide.