Brands

<h2 dir="ltr"> What Are Retroviral Control &amp; Reporter Constructs?</h2>
 Every retroviral over-expression experiment requires a matched set of control constructs to confirm that transduction was successful, the packaging system produced infectious virus at an adequate titer, and the empty vector backbone does not itself alter the measured phenotype. Retroviral control and reporter vectors use the same LTR and backbone configuration as experimental over-expression vectors. Reliable controls and reporters improve confidence in transduction and expression studies. Select the control/reporter system appropriate for your experiment.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="w0hi5b" data-start="4179" data-end="4269">Retro-CMV Blank Retroviruses used as baseline controls for retroviral delivery systems</li>
<li data-section-id="ut4vdp" data-start="4270" data-end="4370">Fluorescent Reporter Retroviruses (GFP, RFP) for real-time monitoring of transduction efficiency</li>
<li data-section-id="izipmv" data-start="4371" data-end="4456">Bicistronic GFP Reporter Vectors for co-expression and experimental normalization</li>
<li data-section-id="we28ta" data-start="4457" data-end="4544">Epitope-tagged Control Vectors supporting protein detection and expression analysis</li>
<li data-section-id="47lirb" data-start="4545" data-end="4642">Stop-codon Reporter Plasmids for assessing baseline expression and vector background activity</li>
<li data-section-id="1d2i69z" data-start="4643" data-end="4735">CMV-driven control plasmids used in retroviral expression and stable cell line workflows</li>
</ul>
<h2 dir="ltr"> How to Choose Retroviral Control &amp; Reporter Constructs</h2>
 Empty Vector Backbone Matching The empty vector control must match the experimental vector in backbone, LTR configuration, internal promoter, and selection marker &mdash; for pBABE experiments, pBABE-puro empty is correct; for pLNCX2 experiments, an empty pLNCX2 vector is required. Mismatched controls introduce confounding variables. Transduction Efficiency Verification GFP-expressing retroviral controls (pBABE-GFP, pMSCV-GFP) enable real-time FACS-based quantification before proceeding with experimental constructs &mdash; Adequate efficiency is typically assessed empirically based on cell type and experimental design, ensuring that packaging and infection conditions are suitable for downstream applications. Histochemical Detection Needs pLAPSN, derived from the pLXSN backbone, expresses bacterial alkaline phosphatase (AP), detectable histochemically with substrates such as NBT/BCIP &mdash; useful when fluorescence-based detection is not available or appropriate, particularly in fixed tissue sections. In Vivo Tracking Requirements Retroviral GFP and luciferase reporter cell lines support in vivo tracking of transplanted cells in mouse models, including haematopoietic reconstitution studies and tumour xenograft experiments via bioluminescence imaging. Multiplexing Considerations Retroviral GFP reporters are compatible with red-channel markers (RFP, mCherry, tdTomato) in multiplexed imaging and FACS &mdash; ensure control and experimental constructs use spectrally distinct reporters. 
<h2> Specifications Context</h2>
 Retroviral control and reporter systems using Retro-CMV and pRetroG-CMV vectors deliver CMV-driven expression cassettes packaged as replication-incompetent retroviral particles for transduction of actively dividing mammalian cells. These systems support stable genomic integration of GFP/RFP reporters, HA-tagged constructs, bicistronic IRES-based expression formats, and empty vector controls for baseline comparison and experimental normalization. Following transduction, integrated expression is maintained through cell division under selection or reporter-based enrichment, enabling consistent gene expression analysis, functional studies, and stable cell line generation in retroviral workflows. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get advanced retroviral control/reporters for your lab today.

Control/Reporter

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Retro-CMV-GFP-IRES-GFP Retrovirus, RVP004

Retro-CMV-GFP-IRES-GFP Retrovirus

RVP004 by Applied Biological Materials is a pre-packaged CMV-GFP-IRES-GFP replication-incompetent retroviral virus (MoMuLV-based). Dual GFP expression from CMV promoter and IRES. For stable GFP transduction control in actively dividing mammalian cells. Store at −80°C in small aliquots.

Unit Quantity

Vector Map

Titer

System

Shipping Condition

Retro-CMV-RFP Retrovirus, RVP005

Retro-CMV-RFP Retrovirus

RVP005 by Applied Biological Materials is a pre-packaged CMV-RFP replication-incompetent retroviral virus (MoMuLV-based). Supplied as 1 mL at 107 IU/mL. For stable RFP expression in actively dividing mammalian cells. Red-fluorescence retroviral transduction control. Store at −80°C in small aliquots.

<h2 dir="ltr">What Are Retroviral Cloning Vectors?</h2>
 The transfer plasmid defines the cargo, promoter, and selection strategy of a retroviral vector system. Selecting the right cloning vector backbone determines transgene expression level, integration stability across cell types, and compatibility with available packaging cell lines. Efficient vector development starts with the right cloning backbone. Choose a retroviral vector that supports your research needs.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="po69uo" data-start="658" data-end="756">Retro-Combo Mix vectors for streamlined retroviral construct assembly and expression workflows</li>
<li data-section-id="5ibmf5" data-start="757" data-end="857">pRetro-E1 and pRetro-E2 plasmid backbones for retroviral vector construction and gene expression</li>
<li data-section-id="1y6pp3s" data-start="858" data-end="961">pRetro-E1-HA and pRetro-E2-His tagged vectors for protein detection and downstream characterization</li>
<li data-section-id="gs2x4g" data-start="962" data-end="1091">Retro-Easy1, Retro-Easy2, Retro-Easy-HA, and Retro-Easy-His systems for flexible construct design and expression optimization</li>
<li data-section-id="fzbhcl" data-start="1092" data-end="1178">Pack-Easy Cells for efficient retroviral packaging and vector production workflows</li>
</ul>
<h2 dir="ltr"> How to Choose a Retroviral Cloning Vector</h2>
&nbsp;
Backbone and Expression Architecture
Retroviral cloning vectors in the Retro-Easy and pRetro-E systems utilize CMV-driven expression cassettes for transgene expression following genomic integration. Backbone configuration influences expression consistency, cloning flexibility, and compatibility with downstream packaging workflows.
Promoter Configuration
CMV-based internal promoters provide strong, constitutive expression across a wide range of mammalian cell types. Expression levels may vary depending on the integration site and chromatin environment following retroviral integration.
Packaging Workflow Compatibility
Retroviral cloning vectors are designed for use in standard replication-incompetent packaging systems, where transfer plasmids are supplied alongside packaging components to generate infectious retroviral particles for transduction of dividing cells.
Selection and Tag Options
Vector variants include antibiotic selection markers and epitope-tagged formats (HA, His) to support downstream detection, purification, or functional validation of expressed proteins.
Insert Size Considerations
Gamma-retroviral transfer vectors generally support moderate-sized inserts suitable for most cDNA and reporter constructs, with efficiency influenced by insert length and sequence composition. 
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<h2 dir="ltr">Specifications Context</h2>
&nbsp;
Retro-Easy and pRetro-E vector systems are based on CMV-driven retroviral transfer backbones designed for stable gene delivery in actively dividing mammalian cells. Following packaging, replication-incompetent retroviral particles mediate genomic integration of the transgene, enabling stable expression across cell divisions. Available formats include untagged, HA-tagged, and His-tagged expression vectors, as well as modular backbone variants for flexible construct design. Pack-Easy Cells provide a dedicated packaging platform for efficient retroviral particle production and consistent vector output. These systems support applications in gene expression analysis, functional studies, and stable cell line generation.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and find the right Retrovirus Cloning Vectors for your lab.

Cloning Vectors

Retro-Combo Mix

E-510 by Applied Biological Materials is a 75 µg ready-to-use second-generation retroviral packaging plasmid combo mix (pRetro-Gag-Pol + pRetro-VSVG). Add transfer plasmid + co-transfect into HEK293T for high-titer VSV-G pseudotyped MMLV retrovirus production. For stable gene delivery in dividing cells. Store at −20°C or below.

Note

Retrovirus Expression Systems Protocol

Retro-Easy-HA

E-518 by Applied Biological Materials is the complete Retro-Easy-HA&trade; retrovirus expression system (1 Kit) for stable delivery and expression of HA-tagged transgenes in dividing mammalian cells via replication-incompetent retrovirus. Includes HA-tag vector with Puromycin selection. Use with Pack-Easy Cells (E-511). Retrovirus Expression Systems Protocol and Drug Selection Killing Curve downloadable. Store at &minus;20°C.

Vector

Retro-Easy-His

E-519 by Applied Biological Materials is the complete Retro-Easy-His&trade; retrovirus expression system (1 Kit) for stable delivery and expression of His-tagged transgenes in dividing mammalian cells. Includes pRetro-E2-His vector (same as E-517) with 6× His tag for Ni-NTA purification and anti-His detection. Use with Pack-Easy Cells (E-511). Store at &minus;20°C.

Retro-Easy1

E-512 by Applied Biological Materials is the complete Retro-Easy1™ retrovirus expression system (1 Kit) for stable gene delivery into dividing mammalian cells. Includes the pRetro-E1 vector with MCS and Neomycin (G418) selection. Use with Pack-Easy Cells (E-511) for streamlined high-titer retroviral production. Retrovirus Expression Systems Protocol and Drug Selection Killing Curve downloadable from the product page.

Retro-Easy2

E-513 by Applied Biological Materials is a complete Retro-Easy2 retrovirus expression system for stable gene delivery into dividing mammalian cells. Achieves titers up to 109 IU/ml using a replication-incompetent MoMuLV backbone with CMV promoter. Includes protocol support and is compatible with ViralEntry™ (G515) to maximize transduction efficiency.

<h2 data-section-id="6b0hnw" data-start="102" data-end="151">What are SV40T antigen overexpression viruses?</h2>
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SV40T antigen overexpression viruses are recombinant lentiviral particles carrying the simian virus 40 (SV40) large T antigen gene, a hexamer protein that inactivates the p53 and retinoblastoma (Rb) tumor suppressor pathways, bypassing the senescence checkpoints that normally limit primary cell proliferation. Some constructs deliver the large T antigen alone, while others include both large and small T antigens. Related entities include p53/Rb pathway inactivation, broad cross-species immortalization (human, mouse, rat, bovine, porcine, canine), karyotype/tumorigenicity characterization (soft agar assay), and antibiotic/fluorescent selection markers.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Lenti-SV40 (tsA58 Mutant) for cell proliferation with inducible control and temperature-sensitive expression</li>
<li dir="ltr" role="presentation">Lenti-SV40T (Puro/Neo/No Marker) provides stable integration with versatile selection markers of highly concentrated lentiviral stocks&nbsp;</li>
<li dir="ltr" role="presentation">Adeno-SV40 Adenovirus for transient T-antigen expression with high-efficiency adenoviral vectors</li>
<li dir="ltr" role="presentation">Retro-SV40 &amp; Retro-SV40LT for special integration in proliferating cells.</li>
<li dir="ltr" role="presentation">Lenti-SV40Tt Lentivirus: Small T and Large T antigens for improved transformation.</li>
</ul>
&nbsp;
<h2 data-section-id="ykynp3" data-start="1032" data-end="1092">How to choose SV40T antigen overexpression virus products</h2>
&nbsp;
Large T antigen alone vs. large and small T antigen
Constructs delivering large T antigen alone target p53/Rb pathway inactivation specifically, while large-and-small-T constructs include additional SV40 small T antigen activity affecting protein phosphatase 2A (PP2A) signaling; confirm which form a published protocol specifies, since the two can produce different immortalization efficiencies and phenotypes.
Species applicability
SV40 large T antigen has documented immortalization success across human, mouse, rat, bovine, porcine, and canine primary cells, making it a broadly applicable option when hTERT (human-specific) is not suitable for the target species.
Selection marker and promoter
As with TERT lentivirus, SV40T constructs are available under CMV or EF1-alpha promoters with antibiotic resistance (puromycin, hygromycin, blasticidin, neomycin/G418, zeocin) or fluorescent-antibiotic fusion markers (GFP-Bsd, RFP-Puro); select based on the target cell line's existing markers and expected culture duration.
Post-immortalization characterization
Because SV40 large T antigen inactivates tumor suppressor pathways, immortalized lines should be characterized for karyotype changes and tested by assays such as soft agar colony formation and tumorigenicity testing if downstream applications require confirmation that the line has not undergone malignant transformation.
&nbsp;
<h2 data-section-id="1fljvas" data-start="3482" data-end="3507">Specifications context</h2>
&nbsp;
SV40T antigen lentivirus is typically supplied at titers around 1x10^8 TU/mL, shipped on dry ice and stored at -80&deg;C, with constructs validated by full-length sequencing and confirmed free of common contaminants. Immortalized lines derived from SV40T transduction are commonly characterized by Western blot or immunofluorescence confirming SV40-LT protein expression, alongside marker expression comparison to the original primary cells. As of 2026, SV40 large T antigen remains the most broadly cross-species-applicable immortalization approach, often selected when hTERT (human-specific) is not an option for the target species or when faster immortalization is prioritized over preserving the original phenotype.
This sub-category sits within MBP's cell immortalization catalog alongside TERT overexpression viruses and additional immortalization agents. Labs constructing custom SV40T expression vectors can also review MBP's cloning reagents. MBP's specialist team can help confirm species applicability and vector format for a specific primary cell type before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to get high-quality SV40T antigen overexpression viruses.

SV40T Antigen Overexpression Viruses

MSDS

Lentiviral Vector Amplification - Protocol

Lentivector Packaging - Protocol

Lentivirus Infection - Protocol

Cell Immortalization - Handbook

Cell Immortalization Reagents - FAQ

Retro-SV40 Retrovirus

Retro-SV40 Retrovirus (G212) by Applied Biological Materials is a replication-incompetent retrovirus at 10⁸ IU/ml supplied as 5 × 50 µl, co-expressing SV40 large T and small t antigens for stable immortalization of actively dividing primary mammalian cells. Store at –80°C.

Description

Application

Expression System Type

Caution

Retro-SV40LT Retrovirus

Retro-SV40LT Retrovirus (RVP010) by Applied Biological Materials is a replication-incompetent retrovirus at 10⁸ IU/ml supplied as 5 × 50 µl, expressing SV40 large T antigen (SV40LT) for stable genomic integration and immortalization of actively dividing primary mammalian cells. Store at –80°C.

Unpacking and Storage Instructions

<h2>What Are Spin-Column Viral DNA Extraction Kits?</h2>
&nbsp;
Spin-column viral DNA extraction kits combine a chaotropic lysis and binding buffer with a silica-membrane spin column housed in a collection tube, allowing viral DNA to be purified using a standard laboratory microcentrifuge. Following lysis, viral nucleic acids bind to the membrane while contaminants are removed during wash steps before purified DNA is eluted for downstream analysis.&nbsp;Choose a spin-column viral DNA extraction kit when processing low-to-moderate sample volumes and when a standard benchtop microcentrifuge provides a more practical solution than a 96-well plate workflow.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Obtain high-quality viral nucleic acids using spin-column-based purification for PCR, qPCR, sequencing, and molecular diagnostic applications.</li>
<li>Mini and maxi purification formats for efficient concentration and isolation of adenovirus, lentivirus, retrovirus, and adeno-associated virus (AAV).</li>
<li>Optimized workflows that minimize inhibitors and maximize downstream compatibility for fast, reproducible recovery of viral nucleic acids.</li>
<li>Scalable purification options supporting both low-throughput and high-throughput virology, infectious disease, and molecular biology research.</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Viral DNA Extraction Kit</h2>
&nbsp;
Input Sample Volume and Processing Capacity
Most spin-column viral DNA extraction kits accept up to 400 &micro;L of liquid sample per column, with some protocols allowing the same column to be reloaded to process larger total sample volumes. Confirm that your sample type and volume fall within the manufacturer's recommended specifications.
Centrifuge Compatibility
Spin-column purification workflows generally require centrifugation speeds of approximately 10,000 &times; g or greater. Most standard benchtop microcentrifuges used with 1.5&ndash;2 mL tubes can accommodate these requirements, but equipment compatibility should always be verified before implementation.
Elution Buffer Versus Water
Many kits provide the option of eluting DNA with either a storage buffer or DNase/RNase-free water. Elution buffer may be preferable for longer-term storage, while water can be advantageous for downstream applications sensitive to EDTA or other buffer components.
Sample Type Considerations
Spin-column viral DNA extraction kits are optimized primarily for cell-free liquid samples. Protein-rich or solid biological samples may require additional pretreatment steps or may be better served by dedicated genomic DNA extraction kits designed for blood or tissue specimens.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The most important performance specifications for spin-column viral DNA extraction kits are sample input capacity and minimum elution volume, as these directly influence DNA concentration and recovery efficiency. A workflow processing several hundred microliters of sample and eluting into a smaller final volume can significantly improve target concentration for downstream qPCR analysis.&nbsp;Many laboratories maintain both spin-column and 96-well plate extraction formats to accommodate varying weekly sample volumes. Spin-column systems offer flexibility and simplicity for smaller studies, while maintaining the DNA quality required for PCR, qPCR, sequencing, and molecular diagnostic applications.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team </a>today to discuss your viral DNA extraction workflow and receive expert guidance on selecting the most suitable spin-column kit for your PCR, qPCR, sequencing, or molecular diagnostics application.

Spin-Column

Product Literature

Retrovirus Purification Maxi Kit

Retrovirus Purification Maxi Kit (V1272-00) by MBP purifies recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 1&times;1013 viral particles from 6&ndash;8 T75 flasks. Available in 2 (V1272-00), 4 (V1272-01), and 10 (V1272-02) prep sizes. Biologically hazardous.

Manufacturer

Retrovirus Purification Maxi Kit, 2 Preps

Retrovirus Purification Maxi Kit, 4 Preps

Retrovirus Purification Maxi Kit, 10 Preps

Retrovirus Purification Mini Kit

Retrovirus Purification Mini Kit (V1172-00) by MBP purifies recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 3&times;1012 viral particles from 1&ndash;2 T75 flasks. Available in 2 (V1172-00), 10 (V1172-01), and 20 (V1172-02) prep sizes. Biologically hazardous.

Retrovirus Purification Mini Kit, 2 Preps

Retrovirus Purification Mini Kit, 10 Preps

Retrovirus Purification Mini Kit, 20 Preps

Electrophoresis System Accessories

User Manual

Accuris myGel Mini electrophoresis-MyGel Mini Comb

Reversible Combs, 18/10 teeth, for E1101-CS2 Casting Stand, pk 2

E1101-COMB2 by Accuris are double-sided reversible gel casting combs with 18 wells on one face and 10 wells on the other, for the E1101-CS2 casting stand of the myGel Mini Electrophoresis System. Multi-channel pipette-compatible. Sold as 2 combs per pack, they serve as direct replacement combs for the E1101 system or expand capacity for labs running dual-tray gel setups.