Brands

Universal Centrifuge Accessories

<h2>What Are Universal Centrifuge Accessories?</h2>
&nbsp;
Universal centrifuge accessories are the interchangeable components that determine the range of applications a universal centrifuge can perform. These include swing-out rotor buckets for blood tubes, conical tubes, microplates, and large bottles; fixed-angle rotors for high-speed pelleting; hematocrit and cytospin rotors; tube adapters; and aerosol-tight bio-containment lids. Selecting the appropriate accessories allows a single centrifuge to support multiple laboratory workflows efficiently and safely.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
Tube adapters and inserts for compatibility with multi-volume samples&nbsp;
</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
Fixed-angle and swing-out rotors for varied centrifugation applications
</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
Lids and sealed buckets for safe and reliable operations
</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
PCR adapters and microplate carriers for high-throughput workflows
</li>
<li dir="ltr" style="font-family: helvetica, arial, sans-serif;" aria-level="1">
Centrifuge tubes, holders, and supporting aids for efficient and complete setups
</li>
</ul>
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<h2>How to Choose Universal Centrifuge Accessories</h2>
&nbsp;
Swing-Out Bucket Selection by Application
Choose swing-out buckets according to the sample formats used in your laboratory. Blood tube buckets support routine clinical applications, conical tube buckets are suitable for cell culture and molecular biology, microplate carriers enable high-throughput workflows, and large-bottle carriers accommodate large-volume sample processing.
Fixed-Angle Rotors
Fixed-angle rotors provide higher centrifugal forces for microtube, PCR tube, and small-volume sample processing. Ensure that both the rotor and centrifuge motor specifications support the required operating speeds and sample formats.
Cytospin Clip Rotors
Cytospin rotors are designed for preparing microscope slides from low-cellularity samples such as body fluids. Verify compatibility with the required funnel sizes and use bio-containment features when processing potentially infectious materials.
Bio-Containment Lids
Aerosol-tight lids help contain biological samples during centrifugation and support safe laboratory practices. Regular inspection of sealing components and O-rings ensures reliable containment and long-term performance.
Reducing Adapter Inserts
Adapter inserts allow smaller tubes to fit securely within larger bucket positions. Using properly matched adapters improves balance, prevents movement during operation, and helps maintain consistent centrifugation results.
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<h2>Specifications Context</h2>
&nbsp;
Modern universal centrifuge systems support interchangeable accessory configurations that enable rapid transitions between clinical, research, cell culture, and cytology workflows. Swing-out buckets, fixed-angle rotors, and microplate carriers provide flexibility while maintaining compatibility with a broad range of vessel formats.
Routine inspection and maintenance of accessories, including adapters, lids, and sealing components, help ensure safe operation and extend equipment lifespan. Advances in bio-containment systems and modular rotor designs continue to improve laboratory efficiency and versatility.
Time to upgrade your universal centrifuge accessories. <a href="https://mbpinc.net/contact-us">Contact the expert team </a>of Molecular Biology Products Inc. (MBP) and the find perfect match today.

46 x 5-7ml insert for Z496-1000-RBUK 2/pk

Z496-1000-A57 by Hermle is a 46 × 5–7 mL tube insert for the Hermle Z496-1000-RBUK deep rectangular centrifuge bucket (2 inserts per pack). It enables the Hermle Z496K ultra high-volume floor centrifuge to process small-format 5–7 mL tubes inside the standard 1000 mL deep rectangular buckets — up to 46 tubes per bucket position — without requiring a rotor change.

Manufacture

Smart Evaporator

<h2>What Are Smart Evaporators?</h2>
&nbsp;
Smart evaporators are laboratory instruments designed for efficient solvent evaporation from one or multiple samples while minimizing bumping and sample loss. Depending on the design, they may use controlled airflow, gentle heating, or other evaporation technologies to accelerate solvent removal while preserving sample integrity. Smart evaporators are compatible with a variety of laboratory vessels and are used in pharmaceutical sample preparation, analytical chemistry, natural product extraction, environmental analysis, and other laboratory workflows requiring efficient solvent removal. Choose a smart evaporator when you need faster, more controlled evaporation than manual evaporation methods, especially when processing multiple samples.
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<h2 class="PDq2pG_selectionAnchorContainer" data-start="0" data-end="23">What You Will Find:</h2>
&nbsp;
<ul data-start="25" data-end="600" data-is-last-node="" data-is-only-node="">
<li data-section-id="ez4bed" data-start="25" data-end="148">Bump-free solvent evaporation systems designed to reduce sample loss and improve recovery during concentration workflows.</li>
<li data-section-id="1v9fno4" data-start="149" data-end="277">Single-channel and multi-channel Smart Evaporator models for processing individual samples or multiple samples simultaneously.</li>
<li data-section-id="1obur8l" data-start="278" data-end="393">Acid-resistant configurations for applications involving corrosive solvents and aggressive chemical environments.</li>
<li data-section-id="xe6o20" data-start="394" data-end="498">Heat carousel options that support faster evaporation and improved throughput across multiple samples.</li>
<li data-section-id="1us20yj" data-start="499" data-end="600" data-is-last-node="">Expansion kits and accessories for configuring, upgrading, and optimizing Smart Evaporator systems.</li>
</ul>
&nbsp;
<h2>How to Choose a Smart Evaporator</h2>
&nbsp;
Sample capacity and configuration
Choose a Smart Evaporator based on the number of samples processed per run and the vessel types used in your workflow. Models range from compact systems for individual samples to multi-channel configurations for higher-throughput solvent evaporation. Verify compatibility with your laboratory tubes, vials, or other sample containers before purchase.
Solvent compatibility
Confirm that the Smart Evaporator is suitable for the solvents used in your laboratory. Standard models are appropriate for many common laboratory solvents, while acid-resistant models are recommended for applications involving corrosive or acidic solvents to improve long-term durability.
Heating options
Some Smart Evaporator models operate at ambient conditions, while others incorporate controlled heating to accelerate solvent evaporation. Select a model with the heating capability appropriate for your solvent properties and sample requirements.
Workflow requirements
Consider whether your application benefits from compact single-sample processing or simultaneous evaporation of multiple samples. Higher-capacity systems improve laboratory throughput for routine sample preparation and analytical workflows.
Accessories and expandability
Check the availability of compatible accessories, such as heating modules, expansion kits, and other system components that can increase capacity or adapt the Smart Evaporator to changing laboratory requirements.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Smart Evaporators are designed to promote efficient solvent evaporation while minimizing bumping and sample loss. Available configurations range from compact single-sample instruments to multi-channel systems for parallel sample processing. Depending on the model, optional heating and specialized accessories can improve evaporation efficiency and expand application flexibility. Acid-resistant models are available for laboratories routinely handling corrosive solvents.
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<a href="https://mbpinc.net/contact-us">Reach out to the MBP team</a> to request a quote for Smart Evaporator systems, accessories, and sample concentration solutions today.

BioChromato 4-Channel Conversion Kit for C1 Smart Evaporators

4Kit for Smart Evaporator C1

C1-4Kit by BioChromato converts the Smart Evaporator C1 into a 4-channel evaporation unit by swapping the head parts and heat block — enabling simultaneous bump-free evaporation of 4 vials. Handles DMSO, DMF, water, and organic solvents via Spiral Plug technology. Made in Japan. Contact customerservice@mbpinc.net for a quote.

Weight (LB)

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Transport Tubes

<h2 dir="ltr">What are Transport Tubes?</h2>
 Transport tubes are sterile, leak-proof screw-cap tubes used for collecting, transporting, and temporarily storing biological specimens from the collection site to the testing laboratory. They span a broad category: empty specimen containers (15 mL and 50 mL self-standing conical tubes), pre-filled saline transport tubes, UTM (Universal Transport Medium) tubes, and specialty specimen vials. All transport tubes share three required attributes&mdash;leak-proof construction, sterility confirmation, and compatibility with the downstream assay's matrix requirements. Choose the transport tube type based on the specimen, the target analyte, and the assay platform it will be processed on. 
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">5 mL transport tubes for routine sample movement</li>
<li dir="ltr" role="presentation">10 mL tubes for higher volume biological samples</li>
<li dir="ltr" role="presentation">Leak-tight screw-cap tubes for secure handling</li>
<li dir="ltr" role="presentation">Sterile transport tubes for clinical workflows</li>
<li dir="ltr" role="presentation">Graduated tubes with labeling surfaces for tracking</li>
<li dir="ltr" role="presentation">Self-standing tubes for easier bench organization</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose Transport Tubes</h2>
 Material: Polypropylene (PP) vs. Polystyrene (PS) PP conical transport tubes are chemical-resistant, autoclavable, and certified DNase/RNase-free in molecular-grade formats. PS transport tubes (30 mL translucent format, Globe Scientific) are lighter and less expensive but not autoclavable. For nucleic acid work, DNA/RNA-free certified PP is the standard. For specimen transport requiring centrifugation, PP is preferred due to higher g-force tolerance (PP rated to &ge;10,000 &times; g in most formats). Self-Standing vs. Rack Format Self-standing (skirted-base) conical tubes stand upright without a rack, reducing knock-over risk during aliquoting and transport. Rack-based tubes require a separate holder. Globe Scientific self-standing 50 mL PP transport tubes feature a printed graduation, white frosted writing area, and are available sterile, non-sterile, or metal-free. Sterile tubes are RNase/DNase-free and pyrogen-free per the lot certificate. Sterile vs. Non-Sterile Sterile tubes (ISO 11137 radiation sterilization, SAL 10⁻⁶) are required for culture, NAAT, and any specimen where contamination would invalidate the result. Non-sterile metal-free PP tubes are suited to trace-element analysis and environmental sampling where sterility is not required but metal contamination would interfere. Volume 15 mL tubes are standard for most clinical specimen aliquots, urine transfers, and culture setups. 50 mL tubes are used for higher-volume specimens (24-hour urine aliquots, stool homogenates, bulk transport of tissue homogenates). Globe Scientific 30 mL tubes with integral spoon are designed specifically for fecal specimen self-collection. Media and Additives Empty transport tubes (no fill) are the base format; select a pre-filled tube (saline, UTM, boric acid) based on the specimen type and analyte stability requirements. For nucleic acid targets, pre-filled tubes with DNA/RNA Shield, UTM, or saline are the appropriate formats. 
<h2 dir="ltr"> Specifications Context</h2>
 Globe Scientific self-standing 50 mL conical transport tubes are manufactured from USP Class VI medical-grade PP with a conical bottom skirt for freestanding stability. Printed graduation marks (1 mL intervals) and a frosted writing area are standard features. Sterile versions are individually wrapped and certified RNase-free, DNase-free, and pyrogen-free per lot certificate, consistent with ISO 9001:2015 manufacturing standards. Non-sterile metal-free versions are supplied with a separate natural screw cap and are tested for trace metals via ICP-MS before lot release. MBP's transport tubes catalog includes Globe Scientific and NEST Scientific formats in multiple pack sizes (25/bag and bulk packs of 500). 
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If you need help choosing tube size or matching the right transport format to your workflow,<a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;"> the MBP team</a> can help you sort it out without overthinking it.

Transport Tube Products

6104SP-Group-Tubes, Caps & Racks Catalog

6104SP-Group-Lab Supplies for Physicians Offices

5 & 10mL Self-Standing Transport Tubes with Printed Graduations

Globe Scientific 6104SP: 5 mL sterile PP self-standing transport tube, 16&times;60 mm, attached white PE screwcap (2-1/4 turn), printed graduations, white writing surface. Freeze-compatible, shatter-proof. 500/case (20/bag &times; 25 bags). Also available as 6105SP (10 mL).

Transport Tube, 5mL, 16 x 60mm, with Attached White Screw Cap,  PP, Conical Bottom, Self-Standing, Sterile, Printed Graduations, 25/Bag, CS/500

Transport Tube, 10mL, 16 x 80mm, with Attached White Screw Cap,  PP, Conical Bottom, Self-Standing, Sterile, Printed Graduations, 25/Bag, CS/500

5 x 1.5ml inserts for Z326-100H rotor, 2/pk.

The Z326-100H-A20 by Hermle is a 5 x 1.5/2.0 ml microtube insert (2/pk) for the Z326-100H hermetically sealed 4 x 100 ml swing-out rotor. Max 5,000 rpm / 3,885 xg on Hermle Z306 / Z326. Enables sealed microtube centrifugation with aerosol containment. Available from MBP Inc.

5 x 50ml conical insert, 2/pk.

The Z446-750-A50-E by Hermle fits the Z446-750 swing-out rotor and holds 5 × 50 ml conical tubes per carrier; the 2-pack delivers capacity for 10 × 50 ml tubes across two rotor buckets.

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
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Zymo Buffers

MSDS - F9001-1/F9001-5

MSDS - F9003

Protocol

5-Fluoroorotic Acid (5-FOA)

F9001-1 by Zymo Research is 5-Fluoroorotic Acid (5-FOA), 1 g powder, ≥98% purity, MW 174 g/mol. The standard URA3 counter-selection agent for yeast genetics — used in plasmid shuffling, allelic replacement, plasmid curing, and two-hybrid screens in Saccharomyces cerevisiae. Store at −20°C.

Format

Molecular Weight

Product Storage

Purity

Solubility

Structure

Manufacturer

Shipping Condition

Storage Condition

5-Fluoroorotic Acid (Powder) (1 g)

5-Fluoroorotic Acid (Powder) (5 g)

100X 5-Fluoroorotic Acid (Liquid) (10 ml)

<h2>What are 5-hmC ELISA kits?</h2>
&nbsp;
5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
&nbsp;
<h2>How to use 5-hmC ELISA kits</h2>
&nbsp;
Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

MSDS

5-Hydroxymethylcytosine DNA Standard (2 µg)

D5405-3 by Zymo Research is a 5-Hydroxymethylcytosine DNA Standard — a 897 bp linear dsDNA in which 100% of cytosines are 5-hmC. Each vial contains 2 µg at 50 ng/µl for use as a positive control or calibration reference in 5-hmC ELISA, dot blot, and LC-MS/MS workflows.

Concentration

Sample Storage

<h2>What are methylated dNTPs?</h2>
&nbsp;
Methylated dNTPs are base-modified deoxynucleotide analogs, the most important being 5-methyl-dCTP (2'-deoxy-5-methylcytidine 5'-triphosphate), in which the cytosine base carries a methyl group at the carbon-5 position. This is the same position methylated in naturally occurring genomic 5-methylcytosine, the primary DNA methylation mark studied in epigenetics research. Substituting 5-methyl-dCTP for standard dCTP in a PCR reaction or in vitro synthesis produces a defined, fully cytosine-methylated DNA template from a known sequence.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
5-Methyl dCTP (10 mM) (100 &micro;l): for high-purity enzymatic incorporation into methylated DNA strands.
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
5-Methylcytosine dNTP Mix (10 mM) (250 &micro;l): pre-validated nucleotide blends for controlled epigenetic assay workflows.
</li>
</ul>
&nbsp;
<h2>How to use methylated dNTPs</h2>
&nbsp;
Substitute 5-methyl-dCTP for dCTP in PCR to synthesize defined methylated DNA
A PCR reaction using 5-methyl-dCTP in place of standard dCTP produces a fully methylated amplicon in which every cytosine carries the 5-methyl modification, providing a positive control with a known and consistent methylation status distinct from the partial, variable methylation of a genomic DNA control.
Use methylated DNA as a positive control for bisulfite or enzymatic conversion workflows
Running 5-methyl-dCTP-synthesized fully methylated DNA through your bisulfite or enzymatic conversion protocol confirms the method correctly preserves methylated cytosines while converting unmethylated ones, catching conversion efficiency problems at the control stage before they affect interpretation of real samples.
Test methylation-sensitive restriction enzymes using 5-methyl-dCTP-produced substrates
Some restriction enzymes cut methylated sequences while others are inhibited by methylation at their recognition site, and a PCR-synthesized, fully methylated substrate of known sequence provides a clean, defined test of whether a specific enzyme is methylation-sensitive or methylation-dependent.
Use as an in vitro substrate for TET enzyme oxidation studies
Since 5-methylcytosine is the starting substrate for TET family enzyme oxidation into 5-hydroxymethylcytosine and further products, fully methylated DNA synthesized with 5-methyl-dCTP provides a defined, homogeneous substrate for in vitro TET enzyme activity assays.
Store methylated dNTPs appropriately to maintain activity
Methylated dNTP analogs are generally stored at -20&deg;C, similar to standard dNTPs, with short-term exposure to ambient temperature acceptable but prolonged room-temperature storage avoided to prevent hydrolysis of the phosphate groups.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
5-methyl-dCTP is typically supplied as a 10 mM solution at pH 7.0 and can be diluted using sterile distilled water or TE buffer, and it can be used in PCR, RT-PCR, and other DNA synthesis applications at the same concentration as standard dCTP in a reaction mix, with 5-methylated DNA probes made this way serving as methylation reference fragments or as pull-down substrates for 5-hydroxymethylcytosine-binding proteins. As of 2026, 5-methyl-dCTP-synthesized control DNA remains a standard tool in epigenetics research labs specifically because it provides a modification-defined substrate without relying on the variable or partially characterized methylation patterns of genomic positive controls.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and get high-quality methylated (dNTPs) for your lab.

Methylated

DataSheet

5-Methyl dCTP (10 mM) (100µl)

Zymo Research D1035: 5-Methyl dCTP at 10 mM, 100 µl (1 µmol). &gt;99% purity by HPLC; free of DNase, RNase, and phosphatase. Compatible with ZymoTaq, QuestTaq, and standard thermostable polymerases. Store at –20°C.

Applicable For 

5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set

The D5405 DNA Standard Set by Zymo Research contains three sequence-identical 897 bp dsDNA standards at 50 ng/µl (2 µg each): unmodified cytosine (D5405-1), 5-methylcytosine (D5405-2), and 5-hydroxymethylcytosine (D5405-3). Ideal reference controls for HPLC, LC/MS, and TLC cytosine modification quantitation studies.

Applicable For

5-Methylcytosine DNA Standard (2 µg)

5-Methylcytosine DNA Standard (2 µg) (D5405-2) by Zymo Research — fully synthetic 897 bp dsDNA with 100% 5-mC at all cytosines. 50 ng/µl, 40 µl (2 µg total) in modified TE buffer. For bisulfite conversion controls, MeDIP, LC/MS, and HPLC assays.

<h2>What are epigenetics enzymes and inhibitors?</h2>
&nbsp;
Epigenetics enzymes and inhibitors are the molecular tools for directly studying or modulating the enzymatic machinery that writes, erases, or reads epigenetic marks. The major classes include: DNA methyltransferases (DNMT1, DNMT3A, DNMT3B), which catalyze the addition of a methyl group to cytosine; TET family enzymes (TET1, TET2, TET3), which oxidize 5-methylcytosine to 5-hydroxymethylcytosine; histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups from histone lysine residues; and histone methyltransferases (HMTs) including EZH2, DOT1L, and G9a. Small-molecule inhibitors of HDAC and DNMT enzymes are the most clinically and experimentally advanced of these tools.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Micrococcal nuclease for nucleosome mapping and controlled chromatin digestion applications</li>
<li dir="ltr" role="presentation">DNA shearing enzymes for consistent fragmentation of DNA in sequencing and epigenetic workflows</li>
<li dir="ltr" role="presentation">DNA degradation enzymes for controlled breakdown and analysis of nucleic acids&nbsp;</li>
<li dir="ltr" role="presentation">CpG methylase enzymes for targeted DNA methylation (CpG-specific )&nbsp; in epigenetic modification studies</li>
<li dir="ltr" role="presentation">5-hmC glucosyltransferase for detection and labeling of hydroxymethylcytosine in DNA</li>
<li dir="ltr" role="presentation">SAM (S-adenosylmethionine) cofactors for supporting enzyme activity and methylation reactions</li>
<li dir="ltr" role="presentation">DNA digestion enzymes for genomic DNA processing workflows</li>
<li dir="ltr" role="presentation">dsDNA nuclease systems for controlled cleavage and analysis of double-stranded DNA</li>
</ul>
&nbsp;
<h2>How to choose epigenetics enzymes and inhibitors</h2>
&nbsp;
Choose DNMT inhibitors to globally reduce or deplete DNA methylation in cells
Nucleoside analog DNMT inhibitors like 5-azacytidine and decitabine incorporate into DNA and trap DNMT1, preventing maintenance methylation and causing passive demethylation through DNA replication, while newer non-nucleoside DNMT inhibitors are in development for more selective, non-genotoxic methylation reduction.
Choose HDAC inhibitors to globally increase histone acetylation
HDAC inhibitors including pan-HDAC hydroxamates like vorinostat (SAHA) or class-selective compounds reduce histone deacetylase activity and cause hyperacetylation of histone lysine residues, broadly promoting gene expression at silenced loci. HDAC inhibitors are the most clinically advanced class of epigenetic therapeutics, with multiple FDA-approved compounds.
Use TET enzyme preparations for in vitro 5-hmC oxidation studies
Purified TET1, TET2, or TET3 enzyme preparations allow controlled in vitro oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products, useful for generating defined 5-hmC-containing substrate DNA or studying TET enzyme activity and selectivity.
Choose EZH2 or other HMT inhibitors for studying PRC2-dependent gene silencing
EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), catalyzes H3K27 trimethylation (H3K27me3), a histone modification associated with repression of tumor suppressor genes, and EZH2 inhibitors like tazemetostat are used in research to probe the role of this silencing mark.
Combine inhibitors to study epistatic relationships between epigenetic marks
Because DNA methylation, histone acetylation, and histone methylation are mechanistically linked, combining HDAC inhibitors with DNMT inhibitors can produce synergistic effects on gene expression exceeding either alone, and these combination approaches are both experimental tools and the basis for combination epigenetic therapy research.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
HDAC and DNMT inhibitors are the most clinically advanced classes of epigenetic therapeutics, with multiple HDAC inhibitors including vorinostat and romidepsin approved for hematological malignancies, EZH2 inhibitors including tazemetostat having received accelerated approval, and multiple BET inhibitors in clinical trials. As of 2026, CRISPR/dCas9 systems fused with DNMTs, TETs, and HDACs enable locus-specific epigenome editing, extending classic enzyme and inhibitor approaches toward targeted epigenetic modulation.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and find the suitable enzymes and inhibitory reagents for your lab.

Enzymes & Inhibitors

5-hmC Glucosyltransferase

E2026 by Zymo Research is 5-hmC Glucosyltransferase supplied at 2 U/µL (100 U). The enzyme specifically transfers glucose to 5-hydroxymethylcytosine in DNA, enabling locus-specific and global 5-hmC quantification via restriction protection or sequencing. Store at −20°C; stable 12 months.

Standard Reaction Time

Storage

Unit Definition

5-hmC Glucosyltransferase (100 U)

5-hmC Glucosyltransferase (200 U)

<h2>What are ELISA kits for methylation in this category?</h2>
&nbsp;
ELISA kits for DNA and RNA methylation in this category are antibody-based assays that quantify the global level of a specific epigenetic mark, most commonly 5-methylcytosine in genomic DNA, 5-hydroxymethylcytosine in genomic DNA, or N6-methyladenosine in RNA, using a plate-based immunoassay format without requiring bisulfite conversion or sequencing. Denatured, single-stranded DNA or RNA from a sample is coated on a plate well, and a mark-specific primary antibody followed by an HRP-conjugated secondary antibody generates a colorimetric signal proportional to the level of the target modification.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">5-mC DNA ELISA kits for quantitative analysis of DNA methylation&nbsp;</li>
<li dir="ltr" role="presentation">DNA controls for validation and calibration of the assay to ensure experimental&nbsp; reproducibility and accuracy&nbsp;</li>
<li dir="ltr" role="presentation">Secondary antibody reagents for specific signal generation and detection in ELISA assays</li>
<li dir="ltr" role="presentation">Coating buffers for consistent assay performance and optimized plate preparation&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose methylation ELISA kits and reagents</h2>
&nbsp;
Match your kit to the specific epigenetic mark you're quantifying
Different kits use antibodies against specific modifications: 5-methylcytosine ELISA kits quantify global DNA methylation, 5-hmC ELISA kits quantify global hydroxymethylation, and m6A ELISA kits quantify RNA N6-methyladenosine. Using a kit with the wrong antibody specificity will not report the mark you intend to measure.
Use methylation standards to build a calibration curve for quantitative results
Running a series of methylation standards with defined percentages alongside your samples allows the absorbance readout to be converted to a specific, meaningful methylation percentage, since the raw ELISA signal alone cannot be interpreted as absolute methylation level without a calibration.
Understand that ELISA measures global levels, not locus-specific methylation
A methylation ELISA gives the overall average percentage of methylation across the genome in a sample, which is useful for comparing broad methylation changes between conditions but cannot identify which specific genes or regions are differentially methylated the way bisulfite sequencing can.
Confirm the required DNA denaturation step is appropriate for your sample type
ELISA kits for DNA methylation typically require single-stranded, denatured DNA coated directly on the plate, and confirming this denaturation step is properly performed is necessary for consistent antibody binding and reproducible signal across wells.
Be aware of cross-reactivity with similar modifications when interpreting results
An antibody against 5-methylcytosine may have some cross-reactivity with 5-hydroxymethylcytosine, and confirming the antibody's published cross-reactivity data helps interpret whether your signal specifically reflects 5-mC, 5-hmC, or both, which matters for studies in cell types where 5-hmC is abundant.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The 5-methylcytosine ELISA workflow uses an indirect ELISA methodology where denatured, single-stranded DNA samples are coated directly on the plate well surfaces and a 5-mC monoclonal antibody and an HRP-conjugated secondary antibody are added sequentially, generating a colorimetric signal proportional to the total level of methylated cytosine in the sample. As of 2026, methylation ELISA kits are particularly valued for high-throughput screening studies comparing global methylation levels across many treatment conditions or patient groups where single-base-resolution data is not the primary goal.
This category sits within DNA/RNA Methylation, alongside Methylation Standards for calibration, and is related to DNA Hydroxymethylation and Non-Bisulfite Kits &amp; Reagents for higher-resolution methylation analysis. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team of&nbsp; Molecular Biology Products Inc. (MBP)</a> to get high-quality ELISA Kits and Reagents for your lab.

D5325-1-15/D5325-1-30 - MSDS

5-mC Coating Buffer

D5325-1-15 5-mC Coating Buffer by Zymo Research — 15 ml, stored at 4°C — coating medium for denatured ssDNA in 5-methylcytosine quantification ELISA assays. Compatible with 5-mC DNA ELISA Kit (D5325, D5326).