Brands

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
</li>
<li dir="ltr" aria-level="1">
Targeted fluorescent labeling of specific cellular structures using viral delivery.
</li>
<li dir="ltr" aria-level="1">
Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
<li dir="ltr" aria-level="1">
Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

Pwpxl-NFAT-mCh

AX0010027 by Applied Biological Materials is a pWPXL lentiviral plasmid (1.0 µg) expressing mCherry under NFAT response elements. Provides a red-fluorescent, quantitative readout of NFAT transcriptional activity for T cell activation studies, NFAT pathway reporter assays, drug screening, and live-cell imaging of NFAT dynamics. mCherry excitation ~587 nm, emission ~610 nm.

Unit Quantity

Storage Condition

Shipping Condition

Pwpxl-ORAI1-(Opto-CRAC)2 vector

AX0010026 Pwpxl-ORAI1-(Opto-CRAC)2 Vector by Applied Biological Materials is a pWPXL lentiviral plasmid encoding ORAI1 fused to two Opto-CRAC light-sensing modules for optogenetic CRAC-mediated calcium entry. Must be co-expressed with AX0010025 (mCh-Opto-CRAC) to reconstitute the complete Opto-CRAC system. Store at &minus;20&deg;C or below.

Pwpxl-mCh-Opto-CRAC vector

AX0010025 Pwpxl-mCh-Opto-CRAC Vector by Applied Biological Materials is a pWPXL lentiviral plasmid encoding the mCherry-fused Opto-CRAC component for light-activated CRAC channel calcium entry. Use together with AX0010026 (Pwpxl-ORAI1-(Opto-CRAC)2) to reconstitute the complete two-component optogenetic CRAC system. Store at &minus;20&deg;C or below.

Cell Counter Accessories

<h2>What Are Cell Counter Accessories?</h2>
&nbsp;
Cell counter accessories are the consumable reagents and replacement components that enable continued, accurate operation of automated cell counters. They fall into three categories: consumable slides (single-use counting chambers loaded fresh for each sample); staining reagents (trypan blue, AO/PI kits, or other viability dyes used to prepare the cell suspension before counting); and instrument maintenance components (calibration beads for verifying counting accuracy, replacement light sources or LED modules, optical cleaning solutions for direct-pipette instruments, and software updates). Counting slides are the dominant consumables by volume and cost, typically used at one slide per sample (two counting chambers per slide provide technical duplicates). Because counting slides are instrument-specific (not interchangeable between brands), procurement must specify the correct instrument model. Third-party slides compatible with certain instruments (Countess-type, TC20-type) are available from alternative suppliers at a lower cost per slide; confirm compatibility and counting accuracy before switching from OEM slides. The two consumables needed for every cell count are a counting slide and a staining reagent; order both in sufficient quantity for your weekly sample volume, and maintain a stock of 4 to 6 weeks of slides to avoid workflow disruptions from supply delays.
&nbsp;
<h2>What You Will Find</h2>
&nbsp;
<ul>
<li>Disposable QuadSlides for accurate and consistent cell counting with QuadCount automated cell counters.</li>
<li>Standard test slides for instrument verification and performance checks.</li>
<li>Barcode scanners for streamlined sample identification and workflow management.</li>
<li>Cell counter accessories designed to support routine operation and maintenance of QuadCount automated cell counters.</li>
</ul>
&nbsp;
<h2>How to Choose Cell Counter Accessories</h2>
&nbsp;
Counting slides: OEM vs. third-party
Original equipment manufacturer (OEM) counting slides are validated for accuracy on the specific instrument and are the safest choice for regulated and research-critical workflows. They typically cost 0.80 to 2.00 USD per slide. Third-party alternative slides (for Countess II-compatible, TC20-compatible, and other common platforms) are available at 0.20 to 0.60 USD per slide, a significant cost reduction in high-frequency counting labs. Before switching to third-party slides, count the same samples on both OEM and third-party slides across a range of densities (1&times;105 to 5&times;106 cells/mL) and viability levels (above 90% and 70 to 80%) to verify equivalent accuracy and viability discrimination.
Trypan blue reagent: concentration and quality
Trypan blue for cell counting is supplied as a 0.4% solution (w/v) in phosphate-buffered saline. Dilute the cell suspension 1:1 with 0.4% trypan blue immediately before counting (resulting in 0.2% trypan blue in the counted mixture). Ensure the trypan blue solution is sterile-filtered (0.2 micron) and stored at room temperature away from light; degraded or contaminated trypan blue causes inconsistent staining intensity, compromising viability discrimination. Some automated cell counters use a proprietary pre-diluted trypan blue optimized for the instrument's imaging algorithm; confirm whether the instrument requires instrument-specific trypan blue or accepts a standard 0.4% solution.
AO/PI viability kits
Acridine orange (AO) and propidium iodide (PI) reagent kits are supplied as ready-to-use or concentrate formulations. AO penetrates all nucleated cells and fluoresces green (excitation 470 to 490 nm, emission 515 to 530 nm); PI enters only cells with compromised membranes and fluoresces red (excitation 530 to 550 nm, emission 610 to 640 nm). AO/PI kits are matched to the fluorescence filter configuration of the specific cell counter; confirm that the instrument has the appropriate excitation/emission channels before specifying a kit. Store AO/PI solutions protected from light; AO is stable for 12 months at 2 to 8 degrees C.
Calibration beads
Calibration bead sets contain fluorescent microspheres at known concentrations and known fluorescence intensities, used to verify that the cell counter's counting algorithm and fluorescence channels are performing within specification. Calibration should be performed at a defined frequency (monthly or quarterly, depending on the laboratory's quality management requirements) and after any instrument relocation, service, or software update. Store calibration beads at 2 to 8 degrees C protected from light; do not freeze.
Direct-pipette instrument cleaning accessories
Cell counters with direct-pipette optical counting surfaces (no disposable slide) require cleaning with manufacturer-specified optical surface cleaning solutions between every sample to prevent carryover. Cleaning kits typically include a low-detergent optical cleaning solution, lint-free optical wipes, and a rinse solution. Follow the manufacturer's cleaning protocol exactly; using inappropriate cleaning agents on the optical surface degrades the surface coating and permanently impairs counting accuracy.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Counting slide chamber depth (most commonly 100 micrometers) determines the volume of suspension imaged per frame and affects cell density calculation. A non-standard chamber depth produces systematic counting errors; never use counting slides with unspecified or incorrectly specified chamber depths for quantitative cell density measurements. Trypan blue should not be left in contact with cells for more than 3 to 5 minutes before counting; prolonged exposure causes staining of live cells with compromised membranes from extended trypan contact, artificially reducing apparent viability. Set up the staining step immediately before counting, not in advance.
&nbsp;
<div class="pointer-events-none -mt-px h-px translate-y-[calc(var(--scroll-root-safe-area-inset-bottom)-14*var(--spacing))]" aria-hidden="true">To discuss product availability or get selection guidance,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.</div>

QuadSlides for QuadCount Cell Counter, pack of 50

E7500-S1 by Accuris Instruments are precision-molded, 4-chambered disposable slides with a hydrophilic inner surface and engraved grid pattern, designed exclusively for the E7500 QuadCount Automated Cell Counter. Each chamber holds a 20 µL sample, enabling four independent counts — or four replicates — from a single loading event. Sold in a pack of 50 slides. Order by catalog number E7500-S1 via Quick Order at mbpinc.net/quickorder or at customerservice@mbpinc.net.

Manufacture

2D Rockers

<h2>What are 2D rockers?</h2>
&nbsp;
2D rocking platforms produce a simple, rhythmic back-and-forth tilting motion on a single axis, causing liquid to flow alternately from one end of the platform to the other with each tilt cycle. The simplicity of this motion is its strength: a consistent linear sweep of liquid across the platform surface ensures even exposure of the membrane or gel to the incubation solution on every cycle, with no rotational bias that could cause uneven staining or antibody distribution. 2D rockers are the universal standard for western blotting workflows and gel processing because their motion is gentle, predictable, and highly effective at sweeping liquid uniformly across flat surfaces.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="text-align: left;" role="presentation">BenchBlotter Platform Rocker (Fixed Speed): Simple, reliable rocking for blotting, staining, and destaining.</li>
<li dir="ltr" style="text-align: left;" role="presentation">TubeRocker Compact Rocker (Grooved Mat): Secure mixing of 1.5&ndash;50 mL tubes with fixed speed and tilt.</li>
<li dir="ltr" style="text-align: left;" role="presentation">BenchRocker 2D Rocker (Variable Speed): Adjustable speed and tilt for flexible lab mixing applications.</li>
<li dir="ltr" style="text-align: left;" role="presentation">2D Everlast 247 Rocker: Continuous-duty operation, ideal for use in incubators and cold rooms.</li>
<li dir="ltr" style="text-align: left;" role="presentation">QuadRocker Tiered Rocker: A multi-level rocking system with over 750 sq. in. of usable surface area.</li>
<li dir="ltr" style="text-align: left;" role="presentation">Globe Scientific Fixed Speed Tube Rocker: Consistent fixed-speed mixing for 1.5&ndash;50 mL tubes.</li>
</ul>
&nbsp;
<h2>How to choose a 2D rocker</h2>
&nbsp;
Confirm the speed range covers your applications
2D rockers typically operate from 5 to 40 rpm, with most blotting applications running at 10&ndash;25 rpm. Some rockers are fixed-speed at a single rpm; variable-speed models provide flexibility to use slower speeds for gentle antibody incubation and faster speeds for wash steps. Confirm that the rocker's speed range accommodates all your planned protocols.
Select a platform size that fits your largest container
Platform size determines the maximum container dimensions: a 25 &times; 30 cm platform accommodates most standard blot boxes and gel staining dishes for common gel formats. For larger gel formats or when running multiple smaller containers simultaneously, a larger platform of 30 &times; 40 cm or more provides the necessary space.
Check the tilt angle adjustment if the rocker offers it
Some 2D rockers allow adjustment of the tilt angle as well as speed, which affects the aggressiveness of the liquid sweep. A larger tilt angle at the same speed produces a stronger sweep but may cause liquid to slosh more vigorously, risking splashing and membrane disturbance at higher angles. For standard antibody incubation, a moderate tilt angle of 10&ndash;20&deg; is typical.
Consider timer and programmable functions for unattended incubations
Some 2D rockers include a built-in timer that switches the rocker off after a set incubation time, preventing over-incubation when the user cannot monitor the step in real time. This is useful for overnight antibody incubations set to run at low speed inside a cold room or refrigerator.
Verify suitability for cold room or incubator use if needed
For overnight antibody incubations at 4&deg;C or hybridization at 37&deg;C or 65&deg;C, a rocker rated for the relevant temperature environment and humidity is required. Standard bench rockers are not always rated for cold room humidity or elevated incubator temperature.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The 2D rocking platform's single-axis tilt produces a consistent, repeatable sweep pattern that has been validated in western blotting protocols across decades of use, making it the most protocol-tested agitation format in molecular biology. As of 2026, variable-speed 2D rockers with a digital display and built-in timer remain the standard configuration in most molecular biology labs where western blotting is performed routinely.
<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> today to request a quote and find the right laboratory rocker for your application.

Product Literature

Stacking platform, small 10.5"x7.5" with flat mat (3.0" separation)

Stacking platform, small 10.5"x7.5" with dimpled mat (3.0" separation)

Stacking platform, large 12"x12" with flat mat (3.0" separation)

Stacking platform, large 12"x12" with dimpled mat (3.0" separation)

Stacking Platform, extra large 14"x12" with flat mat (3.0" separation)

Additional platform separators (4 ea.). Adds 1.25" to platform separation

LaBungee™ 4 cord bungee cord pack 7" to 12" working size

Quadrocker™ Tiered Rocker

The BR5404 by Benchmark Scientific is a four-tier rocking platform with over 750 sq. in. total work surface across four 16 x 12 in. non-slip platforms, operating at 5-80 rpm with user-adjustable 6-22 degree tilt. Cold room and incubator compatible (0-50 degrees C). Ideal for high-volume blot washing, gel staining, and hybridization workflows where bench space is limited but throughput cannot be.

Speed Range

Tilt Angle

Platform Size

Load Capacity

Operating Temp. Range

Dimensions (WxDxH)

Weight

Electrical

Warranty

QuadRocker™ with four non-slip rubber mat platforms, 115V with US Plug

QuadRocker™ with four non-slip rubber mat platforms, 230V with EU Plug

<h2>What are 5-hmC ELISA kits?</h2>
&nbsp;
5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
&nbsp;
<h2>How to use 5-hmC ELISA kits</h2>
&nbsp;
Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

DataSheet

MSDS

Protocol

Quest 5-hmC™ DNA ELISA Kit

The Quest 5-hmC™ DNA ELISA Kit (D5425) by Zymo Research quantifies global 5-hydroxymethylcytosine in 20–200 ng of genomic DNA per well with a sensitivity of ≥0.02% 5-hmC per 100 ng input. The 96-well colorimetric assay completes in 3 hours and is compatible with vertebrate, plant, and microbial DNA, as well as enzyme-digested and sheared fragments.

Detection

Equipment

Input

Sample Source

Manufacturer

Quest 5-hmC™ DNA ELISA Kit (96 Preps.)

Quest 5-hmC™ DNA ELISA Kit (2 x 96 Preps.)

<h2>What Are SARS-CoV-2 RT-qPCR Detection Kits?</h2>
&nbsp;
SARS-CoV-2 RT-qPCR detection kits provide the reverse transcriptase, hot-start Taq polymerase, buffer, and primer/probe sets needed to detect SARS-CoV-2 viral RNA from respiratory specimens by one-step RT-qPCR. Most kits target conserved regions of the viral genome, such as the N (nucleocapsid) gene, alongside a human RNase P (RP) control to confirm sample adequacy.&nbsp;Choose an extraction-based RT-qPCR kit for maximum sensitivity in clinical-style research workflows; choose a kit validated for direct (extraction-free) use when RNA extraction reagent availability or turnaround time is the limiting factor.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>rRT-PCR Detection Kits: Established tools such as the Quick SARS-CoV-2 rRT-PCR Kit, made for specific amplification and dependable detection of viral sequences in both clinical and research specimens.</li>
<li>Multiplex Kits: Efficient multiplexing options that enable the concurrent identification of several viral targets within one reaction to enhance laboratory productivity.</li>
<li>Reverse Transcription Pre-Mixes: Enhanced blends such as ZymoScript RT Pre-Mix, uniquely designed to facilitate the transformation of viral RNA into stable cDNA for further examination.</li>
</ul>
&nbsp;
<h2>How to Choose a SARS-CoV-2 Detection Kit</h2>
&nbsp;
Extraction-Based vs. Extraction-Free Workflows
Standard RT-qPCR workflows use purified viral RNA from an extraction kit as input. Extraction-free (direct) RT-qPCR workflows instead use heat-treated specimen diluent directly as input, avoiding the extraction step entirely. A heat treatment step (commonly 65&deg;C for approximately 10 minutes) lyses virions and inactivates RNases sufficiently for many one-step RT-qPCR master mixes to detect the released RNA.
Master Mix Performance on Direct Samples
Not all one-step RT-qPCR master mixes perform equally on unextracted, heat-treated specimens. Comparative studies have found that some master mix formulations provide substantially higher analytical sensitivity than others when used directly on heat-treated nasopharyngeal swab diluent. Confirm that the specific master mix has been validated for direct-sample use if extraction-free testing is the goal.
Target Gene and Control Design
Kits commonly target a conserved viral gene, such as N1/N2 regions of the nucleocapsid gene used in CDC-style primer/probe sets, alongside a human RNase P control to confirm adequate sample collection and absence of PCR inhibition. Multiplexed detection of both targets in one reaction is standard.
Sample Type Compatibility
Some kits are specifically formulated for unpurified saliva samples, enabling a streamlined extraction-free protocol completed in under an hour. Others are validated primarily for nasopharyngeal swab specimens in viral transport medium. Confirm that sample type compatibility matches your intended specimen source.
Analytical Sensitivity and Validation Data
Published validation studies report limits of detection in the range of approximately 103-104 copies/mL for optimized direct RT-qPCR protocols, with sensitivity, specificity, and accuracy figures commonly reported at 95%+ sensitivity and 98%+ accuracy relative to standard extraction-based methods. Review available validation data when selecting a kit for a specific application.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
In one published evaluation, incubating nasopharyngeal specimens at 65&deg;C for 10 minutes and testing the resulting supernatant directly with a one-step RT-qPCR master mix achieved a limit of detection of approximately 6.6 &times; 103 copies/mL with high reproducibility, and sensitivity, specificity, and accuracy of approximately 95%, 99%, and 98.5%, respectively, relative to a standard RNA extraction-based approach across a panel of clinical specimens.&nbsp;Not all master mix formulations performed equivalently in this direct format. Formulation choice was the dominant variable affecting sensitivity when extraction was omitted. Multiplexed N gene plus human RNase P primer/probe sets, consistent with CDC-style assay design, allow simultaneous target detection and sample adequacy control in one reaction.&nbsp;SARS-CoV-2 RT-qPCR kits are typically stored at -20&deg;C to -30&deg;C and protected from light. Lot-specific Certificates of Analysis (CoAs) are available on request. These products are intended for research use.
&nbsp;
Ensure your diagnostic precision&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> now for a quote on our expert SARS-CoV-2 PCR services.

SARS-CoV-2 Kits

SARS-CoV-2

MSDS (EU)

MSDS (US)

Quick SARS-CoV-2 Multiplex Kit

R3013 by Zymo Research is a multiplex rRT-PCR kit detecting SARS-CoV-2 N gene (HEX) and human RNase P (Quasar 670) simultaneously in a single well from purified respiratory RNA in 2 hours or less. Limit of detection is 167 GEC/ml (10 GEC/reaction). CE-IVD marked. Industry scientists and clinical laboratory professionals benefit from the dual-channel multiplexing format, 384-well scalability, and DNA/RNA Shield-compatible workflow.

Compatibility

Equipment Required

Input Quality

Processing Time

Reagents

Registration Status

Quick SARS-CoV-2 Multiplex Kit (100 Rxns)

Quick SARS-CoV-2 Multiplex Kit (10,000 Rxns)

Quick SARS-CoV-2 Multiplex Kit (1000 Rxns)

Instructions for Use

Healthcare Provider Fact Sheet

Patient Fact Sheet

KingFisher Script

Quick SARS-CoV-2 rRT-PCR Kit

R3011 by Zymo Research is an rRT-PCR kit detecting SARS-CoV-2 viral RNA and human RNase P simultaneously from purified respiratory RNA in 1.5 hours or less. Limit of detection is 250 GEC/ml (15 GEC/reaction). CE-IVD marked for use in the EU. Industry scientists and clinical laboratory professionals running high-throughput SARS-CoV-2 molecular testing benefit from the ready-to-use master mix format and dual-target confirmation design.

Sample Input Material

Quick SARS-CoV-2 rRT-PCR Kit (100 Rxns)

Quick SARS-CoV-2 rRT-PCR Kit (10,000 Rxns)

Quick SARS-CoV-2 rRT-PCR Kit (1000 Rxns)

<h2>What are 16S/ITS NGS library preparation kits?</h2>
&nbsp;
16S/ITS NGS library preparation kits convert extracted microbial DNA into a sequencing-ready library by amplifying a targeted hypervariable region: the 16S ribosomal RNA gene for bacterial and archaeal community profiling, or the ITS (internal transcribed spacer) region for fungal community profiling. Both genes contain conserved flanking sequences that serve as universal PCR primer binding sites, allowing amplification of the variable region in between, which is then sequenced and compared against a reference database for taxonomic classification.
&nbsp;
<h2 dir="ltr">What you will discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-16S&trade; Plus Kits (V1&ndash;V4): Enhanced systems for targeted amplification of bacterial 16S rRNA, incorporating validated reagents for accurate classification.
</li>
<li dir="ltr" aria-level="1">
Quick-ITS&trade; Plus Library Prep: Sensitive kits for fungal detection, employing specialized chemistry to capture ITS regions while preserving genomic integrity at its best.
</li>
<li dir="ltr" aria-level="1">
UDI Indexing Integration: Sophisticated indexing methods to avoid barcode hopping and guarantee precise demultiplexing during simultaneous processing.
</li>
<li dir="ltr" aria-level="1">
Accelerated Workflows: Nuclease-free procedures that reduce manual effort while ensuring transfection-grade purity for delicate sequencing systems.
</li>
</ul>
&nbsp;
<h2>How to choose a 16S/ITS library preparation kit</h2>
&nbsp;
Confirm which hypervariable region(s) your kit targets
16S library prep kits commonly target specific hypervariable regions such as V3-V4, and primer sets re-designed against an updated 16S reference database can meaningfully improve taxonomic coverage compared with older primer designs that may underrepresent certain taxa.
Decide between single-region and full-length amplicon coverage
Short-read sequencing of a single hypervariable region, or a small number of regions, is the standard, cost-effective approach, while long-read platforms can sequence the full-length 16S gene, providing improved species- and strain-level resolution at a different cost and throughput tradeoff.
Plan for PCR chimera formation as a known amplicon sequencing challenge
PCR chimeras, artificial sequences formed from recombination of multiple templates during amplification, are a recognized challenge in amplicon library prep, and kits using real-time PCR to control amplification cycle number can help keep chimera abundance to a low, well-controlled percentage of total reads.
Confirm fungal ITS coverage if your study includes fungal community profiling
Some 16S kits can be run alongside a separate ITS-targeted protocol, or as a combined workflow, to capture both bacterial and fungal community composition from the same sample set, which matters for studies where fungi are a relevant part of the community.
Check hands-on time and workflow complexity for your sample throughput
Some 16S library prep kits are designed for a single-tube, single-reaction workflow with simultaneous indexing and amplification, substantially reducing hands-on time and contamination risk compared with multi-step protocols, which matters for higher-throughput sample sets.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
16S rRNA sequencing requires less input DNA and is less impacted by the presence of host DNA than shotgun metagenomic sequencing, which is part of why it remains the more accessible default for routine bacterial community composition profiling across many labs and sample types. As of 2026, single-tube, real-time-PCR-controlled 16S library prep workflows continue to reduce hands-on time for high-throughput studies while keeping PCR chimera formation to a low, well-controlled percentage of total reads.
<a href="https://mbpinc.net/contact-us">Reach out to the MBP Inc. team</a> today to inquire about a quote for your NGS library preparation requirements.

16S/ITS NGS Library Preparation Kits

Quick-16S Plus NGS Library Prep Kit (V1-V2)

Quick-16S™ Plus NGS Library Prep Kit V1-V2 (D6434-PS1) by Zymo Research — 96-reaction single-PCR kit targeting the V1-V2 region with 30 min hands-on time. Compatible with Illumina MiSeq and NextSeq 1000/2000. No normalization required.

16s V1-V2 Primer Sequences (adapters not included)

Amplicon Size

Barcode Sequences

Index Primers

Required Equipment

Sample Input

Sequencing Platform

Quick-16S Plus NGS Library Prep Kit (V1-V2) with Primer Set 1

Quick-16S Plus NGS Library Prep Kit (V1-V3) with Primer Set 1

Guide for Preparing More Than 96 Samples

Plate Setup Guide

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI)

The D6421-PS1 by Zymo Research is a 96-reaction Quick-16S Plus NGS Library Prep Kit for V3-V4 16S rRNA amplicon sequencing, using a single qPCR/PCR step with Unique Dual Index (UDI) 10-bp barcodes to prepare 96 samples in ~30 minutes hands-on time, with no normalization or gel analysis required. Primer Set 1 (341f/806r) covers 84.2% bacteria and 75.8% archaea; compatible with Illumina MiSeq and NextSeq 2000. Made in the USA.

16S V3-V4 Primer Sequences (adapters not included)

Primer Coverage

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 1

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 2

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 3

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 4

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 5

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 6

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 7

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) with Primer Set 8

Protocol - D6430

Protocol - D6432

Guide for Preparing More than 96 Samples

Quick-16S Plus NGS Library Prep Kit (V4)

D6430-PS1 Quick-16S Plus NGS Library Prep Kit (V4) by Zymo Research prepares 96 indexed Illumina-compatible 16S libraries targeting the V4 region (515–806) using EMP primers, with 30 min hands-on time and MagBead cleanup.

Equipment Needed (user provided)

Index Sequences

V4 Primer Sequences (adapters not included)

Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 1

Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 2

Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 3

Quick-16S Plus NGS Library Prep Kit (V4) (96 preps) with Index Primer Set 4

Quick Protocol -  High Microbial

Quick Protocol - Low Microbial

Quick Protocol - Non-Quant PCR

Guide For > 96 Samples

Quick-16S™ NGS Library Prep Kit

D6400 by Zymo Research converts purified microbial DNA from up to 96 samples into Illumina MiSeq-ready 16S amplicon libraries targeting V1–V2 (~486 bp) or V3–V4 (~596 bp) hypervariable regions. Input is 2 µL of DNA at 5–20 ng/µL; dual barcoding supports 96-plex sequencing. Compatible with standard and low-biomass microbiome protocols.