Brands

<h2>What are non-bisulfite methylation kits and reagents?</h2>
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Non-bisulfite methylation kits and reagents are alternatives to sodium bisulfite conversion that achieve the same goal &mdash; distinguishing methylated from unmethylated cytosines at single-base or regional resolution &mdash; without the harsh chemical treatment that bisulfite introduces. The most widely adopted approach is enzymatic methyl-seq (EM-seq), which uses TET2 to oxidize 5-methylcytosine and 5-hydroxymethylcytosine and T4-BGT to protect them from subsequent APOBEC3A-mediated deamination, so that only unmodified cytosines are converted to uracil.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">qMethyl PCR assay kit uses PCR amplification for quantitative DNA methylation detection</li>
<li dir="ltr" role="presentation">One-step qMethyl PCR system for efficient methylation analysis and optimized workflow setup&nbsp;</li>
<li dir="ltr" role="presentation">Test reaction premixes for validation and optimization of assays&nbsp;</li>
</ul>
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<h2>How to choose non-bisulfite methylation reagents</h2>
&nbsp;
Choose EM-seq for superior library quality over bisulfite at the same resolution
EM-seq outperforms bisulfite-converted libraries on all major quality metrics: higher library yield, fewer duplicates, more usable reads, more even GC distribution, and accurate methylation calling, without requiring any changes to downstream bioinformatic analysis pipelines since the output is the same C-to-T chemistry as bisulfite conversion.
Understand EM-seq's two-step enzymatic mechanism
In the first EM-seq step, TET2 oxidizes 5-mC and 5-hmC to protected forms while T4-BGT glucosylates 5-hmC; in the second step, APOBEC3A deaminates only the unprotected unmodified cytosines to uracil. The net result is the same as bisulfite conversion &mdash; unmethylated C reads as T &mdash; but without bisulfite's destructive chemistry.
Consider TAPS for mild, nondestructive conversion that directly marks modified cytosines
TET-assisted pyridine borane sequencing (TAPS) is a bisulfite-free method that combines TET oxidation of 5-mC and 5-hmC with pyridine borane reduction, converting modified cytosines to a thymine read while leaving unmodified cytosines intact. Unlike bisulfite and EM-seq, TAPS positively marks the modified bases rather than converting the unmodified ones, and can preserve DNA fragments over 10 kilobases.
Choose MeDIP for genome-wide enrichment without per-cytosine resolution
Methylated DNA immunoprecipitation uses an antibody specific to 5-methylcytosine or 5-hydroxymethylcytosine to pull down methylated DNA fragments, providing regional enrichment data at lower cost and without bisulfite-related DNA damage, though at lower resolution than sequencing-based conversion methods.
Confirm whether your method distinguishes 5-mC from 5-hmC or co-detects both
EM-seq detects both 5-mC and 5-hmC together and cannot distinguish them without additional steps, while TAPS also co-detects both; TAPS&beta;, a variant, can specifically call 5-mC by blocking 5-hmC before oxidation. MeDIP can be targeted specifically to 5-hmC (hMeDIP) using an anti-5-hmC antibody.
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<h2>Specifications context</h2>
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EM-seq was effective using as little as 100 pg of DNA input, and libraries showed higher yields with fewer PCR cycles than bisulfite-converted libraries at all input amounts tested, with more even GC distribution, reduced sequencing duplicates, and more CpGs detected within genomic features per sequencing read. As of 2026, enzymatic conversion via EM-seq is broadly recommended over bisulfite conversion for new methylome analysis projects, particularly those working with limited, degraded, or high-value samples where the DNA damage of bisulfite treatment is most consequential.
This category sits within DNA/RNA Methylation, alongside Bisulfite Conversion Kits, and is closely related to DNA Hydroxymethylation for 5-hmC-specific approaches. See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at Molecular Biology Products Inc. (MBP)">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to find dependable non-bisulfite kits and reagents for your lab.

Non-Bisulfite Kits & Reagents

MSDS

2X Test Reaction PreMix

2X Test Reaction PreMix (D5310) by Zymo Research — 500 µl, contains MSREs and SYTO 9 dye for bisulfite-free locus-specific DNA methylation quantification in the OneStep qMethyl Kit. Compatible with Bio-Rad CFX, QuantStudio, and LightCycler 480. Store at −20°C. Made in the USA.

Manufacturer

Shipping Condition

Storage Condition

2X Reference Reaction PreMix

2X Test Reaction-Lite PreMix

The 2X Test Reaction-Lite PreMix (D5311) by Zymo Research is the dye-free bisulfite-free DNA methylation quantitation component for the OneStep qMethyl-Lite system. Uses methylation-sensitive restriction enzymes (MSREs) to calculate percent methylation from qPCR ΔCt values — no bisulfite conversion required. Researcher supplies compatible fluorescent dye or TaqMan probe; input 5 ng–1 µg DNA; amplicons &gt;150 bp.

2X Reference Reaction-Lite PreMix

<h2>What is a 2 kb DNA ladder?</h2>
&nbsp;
A 2 kb DNA ladder provides reference bands spaced at approximately 2-kilobase intervals, designed for gel electrophoresis where samples include large DNA fragments and where 1-kilobase spacing between reference bands would crowd too many bands into the upper region of a low-percentage gel. These ladders are particularly useful when working with large restriction digest products, BAC or cosmid clone inserts, large plasmids, or genomic DNA fragments in the 2&ndash;20 kb size range, run on 0.5&ndash;0.8% agarose to give these large molecules room to resolve.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
PureView PCR DNA Ladder for routine analysis of gel and size estimation of PCR product&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
2kb Opti-DNA Marker for accurate sizing of DNA fragments in gel electrophoresis&nbsp;
</li>
</ul>
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<h2>How to choose and use a 2 kb DNA ladder</h2>
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Use a 2 kb ladder when your samples are predominantly in the 2&ndash;20 kb size range
If most of your samples are in the multi-kilobase range, 2 kb spacing gives each reference band more separation on the gel, making it easier to estimate size in a region where 1 kb reference bands would be compressed together.
Run large-fragment gels on 0.5&ndash;0.8% agarose for adequate resolution
Standard 1% agarose does not give sufficient resolution for DNA fragments above 10 kb, and low-percentage gels of 0.5&ndash;0.8% provide the larger pore size needed for large molecules to migrate and resolve as distinct bands rather than compressing together at the top of the gel.
Confirm your 2 kb ladder covers the upper size range of your samples
Like any DNA ladder, the 2 kb ladder's largest defined band should be at or above the largest fragment in your sample, since fragments larger than the ladder's upper limit cannot be sized against it and may run together at the well or upper gel.
Plan for longer electrophoresis run times with low-percentage gels
Large DNA fragments migrate slowly through gel matrices, and low-percentage gels run at typical voltages require longer run times to achieve adequate separation of bands across the size range covered by the ladder.
Use a 2 kb ladder alongside a 1 kb ladder when samples span a wide size range
If your samples include both small and large fragments, loading both a 2 kb ladder and a 1 kb ladder in adjacent lanes provides reference coverage across the full size range without either ladder being suboptimal for part of your sample set.
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<h2>Specifications context</h2>
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The compression of larger DNA bands in the upper gel region is a fundamental property of agarose electrophoresis: the relationship between fragment size and migration distance is non-linear, so bands in the upper kilobase range are closer together in migration distance than the same size difference produces in the lower range. The 2 kb increment addresses this by providing wider-spaced reference points where 1 kb spacing would produce visually crowded bands on a low-percentage gel. As of 2026, 2 kb ladders are most commonly used when gel conditions are deliberately optimized for large fragments, such as for BAC clone verification or genomic DNA fragment analysis.
<a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to get high-quality 2Kb DNA ladders tailored for your research workflows.

2Kb DNA Ladders

2kb Opti-DNA Marker

DNA Marker Guide

Unit Quantity

Application

Shipping condition

Square Well Deep Well Plates

<h2 data-section-id="7nn2n" data-start="0" data-end="42">What Are Square Well Deep Well Plates?</h2>
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Square well deep well plates are polypropylene microplates designed with square-shaped wells instead of traditional round wells. This design maximizes liquid capacity within the standard SBS/ANSI microplate footprint, making them ideal for high-throughput laboratory workflows that require larger sample volumes. Commonly available in 96-well formats with capacities ranging from 1.1 mL to 2.2 mL per well, these plates are widely used for nucleic acid extraction, protein precipitation, compound library storage, sample preparation, and automated liquid handling.
The square-well geometry increases usable volume while maintaining compatibility with automated platforms from Hamilton, Tecan, Beckman Coulter, and Agilent. Manufactured from virgin polypropylene, square well plates offer excellent chemical resistance to DMSO, ethanol, acetonitrile, and extraction buffers, while minimizing sample binding and contamination risks.
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<h2 dir="ltr">Product Range:</h2>
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<ul>
<li dir="ltr" aria-level="1">
96-square well storage plates for sample management.
</li>
<li dir="ltr" aria-level="1">
2.2ml deep well plates for large-volume handling.
</li>
<li dir="ltr" aria-level="1">
V-bottom square well plates for maximum sample recovery.
</li>
<li dir="ltr" aria-level="1">
DNA-free deep well microplates for molecular applications.
</li>
<li dir="ltr" aria-level="1">
Medical-grade polypropylene plates for chemical durability.
</li>
<li dir="ltr" aria-level="1">
Automation-ready square well formats for robotic integration.
</li>
<li dir="ltr" aria-level="1">
Deep well plates for bead beating and high-impact use.
</li>
<li dir="ltr" aria-level="1">
Chemical-resistant 96-well plates for harsh reagents.
</li>
</ul>
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<h2 data-section-id="1lthnju" data-start="1133" data-end="1179">How to Choose Square Well Deep Well Plates</h2>
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Well Volume The most common formats are 2.0 mL and 2.2 mL square well plates, which provide sufficient volume for nucleic acid extraction, protein precipitation, and compound storage workflows. Lower-profile 1.1 mL plates are useful when deck height limitations or freezer space optimization are important considerations.
Bottom Geometry V-bottom wells concentrate liquid and pellets into a single point, enabling more complete aspiration and improved recovery during centrifugation-based workflows. U-bottom wells promote better mixing and resuspension, making them suitable for microbial culture applications and serial dilutions.
Centrifugation Requirements Most square well polypropylene plates are compatible with centrifugation speeds between 4,000 &times; g and 6,000 &times; g. For demanding extraction protocols, reinforced plate designs provide additional structural stability during high-speed centrifugation.
Automation Compatibility Select plates that comply with ANSI/SLAS standards and include molded alphanumeric indexing for sample tracking. Features such as stacking lugs and raised well rims improve robotic handling and sealing performance.
Certification Standards For molecular biology applications, choose plates manufactured from virgin polypropylene and certified DNase-free, RNase-free, DNA-free, and PCR inhibitor-free. Sterile options are recommended for sensitive workflows and regulated environments.
&nbsp;
<h2 data-section-id="u7fdje" data-start="3248" data-end="3283">Specifications and Applications</h2>
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Square well deep well plates are typically manufactured from high-purity polypropylene and operate across a wide temperature range, often from approximately &ndash;86&deg;C to +125&deg;C. Most plates conform to ANSI/SLAS microplate dimensions (127.76 &times; 85.48 mm), ensuring compatibility with automated liquid handlers, centrifuges, incubators, and storage systems.
Today, 2.0 mL and 2.2 mL square well V-bottom plates remain the preferred format in pharmaceutical research, clinical diagnostics, genomics laboratories, and biobanking facilities due to their high volume capacity, efficient liquid recovery, and seamless integration into automated workflows.
Upgrade your lab with durable essentials that won't fail during critical runs. Browse our website and <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">request a quote from the team at MBP</a> for your supplies.

2ml Deep Well Plate Nunc

MBP20CU by Nunc — 96-well deep well plate, 2 mL/well, U-bottom round wells, non-treated polypropylene, ANSI/SLAS standard footprint. Compatible with robotic liquid handlers and multichannel pipettors. Supplied 50 plates per case.

<h2 dir="ltr"> What Are Lentiviral Purification &amp; Packaging Kits?</h2>
 Producing high-quality lentiviral stocks is a prerequisite for reproducible transduction experiments. Low-titer or impure virus preparations reduce transduction efficiency, introduce toxicity from residual cell debris, and compromise experimental reproducibility. Purification and packaging kits address every step from plasmid transfection through virus harvest and concentration. Vector production quality depends on efficient purification and packaging workflows. Choose the kit designed for your system.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
2nd and 3rd Generation Lentivirus Bundles combining transduction enhancers, transfection reagents, packaging mix, titer kits for complete workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
2nd and 3rd Generation Packaging System Mix for standardized viral production workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
DNAfectin&trade; Plus and Packaging Mix systems for enhanced transfection workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Lentivirus Promoter Blast&trade; Kit for lentiviral expression studies driven by promoters
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
SARS-CoV-2 S Protein Pseudotype Lentiviral Packaging Mix for pseudovirus-based research applications
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
Ultra-Pure Lentivirus Purification Kit for viral cleanup and concentration
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
ViralEntry&trade; Transduction Enhancer for improved transduction
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif; color: #000000;" aria-level="1">
qPCR Lentivirus Titer Kit &amp; ViralEntry&trade; Bundle for optimized transduction workflows and&nbsp; viral quantification&nbsp;
</li>
</ul>
<h2 dir="ltr"> How to Choose a Purification &amp; Packaging Kit</h2>
 Concentration Method Available PEG precipitation kits work with a standard benchtop centrifuge, producing 10&ndash;100-fold concentration &mdash; the most accessible method for labs without ultracentrifuges. Ultrafiltration (100 kDa cutoff) offers high recovery (&gt;80%) while removing small molecules and unincorporated VSV-G. Ultracentrifugation provides the highest concentration (up to 1000&times;) but requires specialised equipment. Packaging Plasmid Generation 3rd generation systems (pMDLg/pRRE + pRSV-Rev + pVSV-G) split packaging elements across three plasmids and eliminate Tat for biosafety. 2nd generation systems use a single packaging plasmid + pVSV-G; some transfer vectors with RSV promoters produce lower titers with 3rd generation packaging unless using CMV-promoter variants (pLKO.1C). Starting Volume Scale Ultrafiltration units are compatible with both small-scale (&le;20 mL) and large-scale (up to 1 L) starting volumes using appropriately sized membranes. Plasmid Transfection Ratio For 3rd generation packaging, a 4:2:1:1 ratio of transfer vector:pMDLg/pRRE:pVSV-G:pRSV-Rev has been validated to maximise titer, with 20 &micro;g transfer vector per 15 cm dish as a standard starting point. Titer Assessment Method p24 ELISA measures total viral particle content; functional transduction assays (GFP or antibiotic selection) measure infectious titer; qPCR-based RNA titration measures packaged RNA copies. Functional titer is most relevant for transduction experiments. 
<h2 dir="ltr"> Specifications Context</h2>
 Lentiviral concentration workflows commonly combine PEG precipitation, ultrafiltration, or density-gradient methods to increase viral titer without requiring ultracentrifugation. Ultrafiltration systems using 100 kDa membranes efficiently retain lentiviral particles (approximately 80&ndash;120 nm) while removing smaller contaminants and free proteins. Following concentration, viral stocks are typically aliquoted into single-use volumes and stored at &minus;80 &deg;C because infectivity decreases with repeated freeze&ndash;thaw cycles and prolonged storage above freezing temperatures. Modern 2nd- and 3rd-generation packaging systems separate essential viral functions across multiple plasmids, including a dedicated VSV-G envelope plasmid, to support safer lentiviral production workflows. Product availability reflects MBP's catalogue as of mid-2026. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. and get reliable lentiviral purification and packaging kits for your lab today.

Purfication & Packaging Kits

DataSheet

2nd Gen. Packaging Mix & DNAfectin™ Plus Combo Pack

Note

MSDS - LV900

MSDS - G2500

2nd Generation Lentivirus Bundle 1 (LV003-Packaging Mix, G2500-Transfection Reagent, LV900-Titer Kit)

Applications

MSDS  - G2500

MSDS - G515

2nd Generation Lentivirus Bundle 2 (LV003-Packaging Mix, G2500-Transfection Reagent, LV900-Titer Kit, G515-Transduction Enhancer)

Lentiviral System FAQ

Selection-Drug Killing Curve

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Enhanced Lentivirus Safety Features-Replication Incompetency

Suggested MOI for Common Cancer Cell Lines

2nd Generation Packaging System Mix

<h2 dir="ltr"> What Is a Taq PCR Master Mix?</h2>
 The 2x Taq PCR master mix format has become the standard for high-throughput molecular biology workflows because it combines reliability, convenience, and reproducibility in a single-tube format. By pre-optimising the concentrations of all PCR components, master mixes reduce pipetting steps and experimental variability compared to assembling reactions from individual components. This simplifies workflow setup and improves reproducibility. Select a formulation designed for your amplification needs.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr">High-Purity Enzymes: Versatile independent polymerases, such as the Azura&trade; and Accuris series, crafted for adaptable reaction adjustments and enhanced processivity with various DNA templates.</li>
<li dir="ltr">Full Reaction Solutions: All-inclusive systems such as the PCR Sure&trade; Kit that feature optimized dNTPs and buffers, offering a ready-to-use option for labs requiring dependable high-throughput performance and consistent outcomes.</li>
</ul>
<h2 dir="ltr"> How to Choose a Taq PCR Master Mix</h2>
 Standard vs. Dye-Containing Standard 2x master mixes require a separate loading dye addition before gel electrophoresis. Dye-containing master mixes include loading dye in the formulation, allowing direct gel loading after amplification &mdash; streamlining routine band-confirmation workflows. Component Pre-Optimisation A 2x Taq PCR master mix is a pre-formulated, double-concentrated solution containing Taq DNA polymerase, dNTPs (typically 200 &micro;M each at 1x), MgCl2, and PCR buffer at optimised concentrations &mdash; requiring only template and primer addition to set up a reaction. Throughput Scale High-throughput genotyping and colony screening benefit most from master mix consistency across multi-well plates, where individual component pipetting would introduce variability and time cost. Storage and Stability Master mixes are typically stable at &minus;20 &deg;C with multiple freeze-thaw cycles, though some dye-containing formulations may have slightly different stability profiles &mdash; check product-specific storage recommendations. Compatibility with Existing Protocols When switching from individual-component reactions to a master mix format, confirm that the pre-set MgCl2 and dNTP concentrations are compatible with your existing primer designs and templates, or adjust accordingly. 
<h2 dir="ltr"> Specifications Context</h2>
 A 2x Taq PCR master mix is set up by combining the master mix with template DNA and primers &mdash; typically at a 1:1 ratio of master mix to combined template/primer/water volume, achieving the final 1x working concentration. This format has become the standard for high-throughput molecular biology because it reduces pipetting steps and lot-to-lot variability across multi-well plate setups, important for reproducibility in genotyping and screening pipelines. Master mixes are typically stored at &minus;20 &deg;C with documented multiple freeze-thaw tolerance. When transitioning a protocol from individual-component PCR to a master mix format, running both formulations side-by-side on the same template and primer set is a useful validation step, confirming that the pre-set MgCl2 and dNTP concentrations in the master mix produce comparable yield and specificity to the previously optimised individual-component reaction before fully adopting the new format across a workflow. Product availability reflects MBP's catalogue as of mid-2026. 
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Enhance your amplification outcomes&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP team </a>now for a quote on our expert Taq Polymerase offerings.

Taq PCR Master Mixes

User Manual

2x Taq PCR Mix

2x Taq PCR Mix (TP01-00) by MBP is a ready-to-use 2× PCR master mix containing Taq at 5 u/µL, 10× Buffer, 20 mM MgSO4, and loading dye. Available in 100 µl (TP01-00), 5×1 mL (TP01-01), and 20×1 mL (TP01-02). Ships on ice.

2x Taq PCR Mix, 100ul

2x Taq PCR Mix, 5 x 1 mL

2x Taq PCR Mix, 20 x 1 mL

<h2 dir="ltr">What is a SYBR Green Dye-Based Master Mix?</h2>
 A SYBR Green dye-based master mix is a 2x, ready-to-use formulation designed for dye-based, quantitative amplification of DNA targets, including cDNA and genomic DNA, by real-time PCR. SYBR Green dye binds to double-stranded DNA formed during amplification, and the resulting fluorescence increases proportionally to the quantity of double-stranded product, providing a real-time readout of amplification progress. Choose a SYBR Green dye-based master mix for cost-effective, flexible quantification across any primer pair and target combination, especially when running many different assays without the overhead of probe design.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="ic0lb3" data-start="180" data-end="319">SYBR Green dye-based qPCR master mixes for real-time quantification of DNA and cDNA targets across a wide range of research applications.</li>
<li data-section-id="1yz7ajv" data-start="320" data-end="449">Fast qPCR formulations with HiROX and LoROX options to support compatibility with different real-time PCR instrument platforms.</li>
<li data-section-id="1fbhq1p" data-start="450" data-end="586">Blue tracking dye master mixes, including AzuraView&trade; GreenFast and Accuris qMAX Green formats, for improved reaction setup visibility.</li>
<li data-section-id="1787obc" data-start="587" data-end="726">One-step SYBR Green RT-qPCR mixes that combine reverse transcription and real-time PCR in a single tube for RNA quantification workflows.</li>
<li data-section-id="1g0jipr" data-start="727" data-end="852">Ready-to-use 2x master mixes formulated for reproducible qPCR setup while reducing pipetting steps and workflow complexity.</li>
</ul>
<h2 dir="ltr"> How to Choose a SYBR Green Dye-Based Master Mix</h2>
 Dynamic Range and Sensitivity Look for master mixes offering a wide linear dynamic range -- some formulations provide precise measurement across approximately six logs of dynamic range, enabling accurate detection across both low- and high-concentration samples in the same assay design. Primer Melting Temperature Flexibility SYBR Green master mixes are commonly formulated to provide flexibility for primers with melting temperatures (Tm) in the range of approximately 55-65 &deg;C, reducing the need for extensive primer-specific optimisation when bringing new assays online. Bench-Top Stability Dual hot-start mechanisms in some SYBR Green master mixes provide extended bench-top stability -- up to 72 hours at room temperature in the dark in some formulations -- with single melt curves confirmed at both initial and post-incubation time points, supporting automated and batched workflows. Carryover Contamination Prevention Formulations that include uracil-DNA glycosylase (UDG) and dUTP help ensure leftover DNA residue from previous reactions is not re-amplified in subsequent experiments, a useful feature for high-throughput repeat assays. Instrument and Workflow Compatibility Broad instrument compatibility - including standard and fast cycling modes -- and compatibility with a variety of reverse transcriptase enzymes (for RT-qPCR workflows using a separately generated cDNA) widen the range of platforms a single master mix can support. 
<h2 dir="ltr"> Specifications Context</h2>
 SYBR Green dye is a fluorescent double-stranded DNA-binding dye that tracks amplification progress in real-time PCR: as the reaction proceeds, the dye binds newly synthesised dsDNA at each extension step, and the resulting fluorescence increase is proportional to product quantity. Because SYBR Green is not PCR-inhibitory and works with any primer-template combination, it provides accurate, reproducible results with high sensitivity across a broad dynamic range and gives consistent results across different real-time PCR platforms - one reason dye-based chemistry remains cost-effective compared to probe-based systems, which require a different labelled probe for each target sequence. SYBR Green master mixes are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs are available on request. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today and get high-yield SYBR/Dye-Based real-time PCR reagents for your lab.

SYBR/Dye-Based

Product Literature

2x qPCR Mix SBGR

2x qPCR Mix SBGR (QP1311-01) by MBP enables highly sensitive detection from few copies of DNA template with a broad dynamic range for accurate quantification of target sequences. Available in 100-reaction (QP1311-01) and 500-reaction (QP1311-02) sizes. Ships on ice.

2x qPCR Mix SBGR, 100 Reactions

2x qPCR Mix SBGR, 500 Reactions

QP1313 Protocol

2x qPCR Mix, SBGR,  Rox

2x qPCR Mix SBGR Rox (QP1313-01) by MBP enables highly sensitive detection from few copies of DNA template with a broad dynamic range for accurate quantification. Standard Rox formulation for instruments requiring a ROX reference. Available in 100-reaction (QP1313-01) and 500-reaction (QP1313-02) sizes. Ships on ice.

2x qPCR Mix, SBGR,  Rox, 100 Reactions

2x qPCR Mix, SBGR,  Rox, 500 Reactions

QP1312 Protocol

2x qPCR Mix, SBGR, Low Rox

2x qPCR Mix SBGR Low Rox (QP1312-01) by MBP enables highly sensitive detection from few copies of DNA template with a broad dynamic range for accurate quantification. Low Rox formulation for instruments requiring a low ROX reference. Available in 100-reaction (QP1312-01) and 500-reaction (QP1312-02) sizes. Ships on ice.