Brands

<h2 data-section-id="6b0hnw" data-start="102" data-end="151">What are SV40T antigen overexpression viruses?</h2>
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SV40T antigen overexpression viruses are recombinant lentiviral particles carrying the simian virus 40 (SV40) large T antigen gene, a hexamer protein that inactivates the p53 and retinoblastoma (Rb) tumor suppressor pathways, bypassing the senescence checkpoints that normally limit primary cell proliferation. Some constructs deliver the large T antigen alone, while others include both large and small T antigens. Related entities include p53/Rb pathway inactivation, broad cross-species immortalization (human, mouse, rat, bovine, porcine, canine), karyotype/tumorigenicity characterization (soft agar assay), and antibiotic/fluorescent selection markers.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Lenti-SV40 (tsA58 Mutant) for cell proliferation with inducible control and temperature-sensitive expression</li>
<li dir="ltr" role="presentation">Lenti-SV40T (Puro/Neo/No Marker) provides stable integration with versatile selection markers of highly concentrated lentiviral stocks&nbsp;</li>
<li dir="ltr" role="presentation">Adeno-SV40 Adenovirus for transient T-antigen expression with high-efficiency adenoviral vectors</li>
<li dir="ltr" role="presentation">Retro-SV40 &amp; Retro-SV40LT for special integration in proliferating cells.</li>
<li dir="ltr" role="presentation">Lenti-SV40Tt Lentivirus: Small T and Large T antigens for improved transformation.</li>
</ul>
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<h2 data-section-id="ykynp3" data-start="1032" data-end="1092">How to choose SV40T antigen overexpression virus products</h2>
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Large T antigen alone vs. large and small T antigen
Constructs delivering large T antigen alone target p53/Rb pathway inactivation specifically, while large-and-small-T constructs include additional SV40 small T antigen activity affecting protein phosphatase 2A (PP2A) signaling; confirm which form a published protocol specifies, since the two can produce different immortalization efficiencies and phenotypes.
Species applicability
SV40 large T antigen has documented immortalization success across human, mouse, rat, bovine, porcine, and canine primary cells, making it a broadly applicable option when hTERT (human-specific) is not suitable for the target species.
Selection marker and promoter
As with TERT lentivirus, SV40T constructs are available under CMV or EF1-alpha promoters with antibiotic resistance (puromycin, hygromycin, blasticidin, neomycin/G418, zeocin) or fluorescent-antibiotic fusion markers (GFP-Bsd, RFP-Puro); select based on the target cell line's existing markers and expected culture duration.
Post-immortalization characterization
Because SV40 large T antigen inactivates tumor suppressor pathways, immortalized lines should be characterized for karyotype changes and tested by assays such as soft agar colony formation and tumorigenicity testing if downstream applications require confirmation that the line has not undergone malignant transformation.
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<h2 data-section-id="1fljvas" data-start="3482" data-end="3507">Specifications context</h2>
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SV40T antigen lentivirus is typically supplied at titers around 1x10^8 TU/mL, shipped on dry ice and stored at -80&deg;C, with constructs validated by full-length sequencing and confirmed free of common contaminants. Immortalized lines derived from SV40T transduction are commonly characterized by Western blot or immunofluorescence confirming SV40-LT protein expression, alongside marker expression comparison to the original primary cells. As of 2026, SV40 large T antigen remains the most broadly cross-species-applicable immortalization approach, often selected when hTERT (human-specific) is not an option for the target species or when faster immortalization is prioritized over preserving the original phenotype.
This sub-category sits within MBP's cell immortalization catalog alongside TERT overexpression viruses and additional immortalization agents. Labs constructing custom SV40T expression vectors can also review MBP's cloning reagents. MBP's specialist team can help confirm species applicability and vector format for a specific primary cell type before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to get high-quality SV40T antigen overexpression viruses.

SV40T Antigen Overexpression Viruses

Lentivirus Infection - Protocol

MSDS

Lentiviral Vector Amplification - Protocol

Lentivector Packaging - Protocol

Cell Immortalization - Handbook

Cell Immortalization Reagents - FAQ

Lenti-SV40 Lentivirus, High Titer

Lenti-SV40 Lentivirus, High Titer (LV612) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing the complete SV40 viral genome, supplied as 2 × 100 µl for cell immortalization, SV40 biology research, viral transformation studies, and host-virus interaction research. Shipped on dry ice; store at –80°C.

Unit Quantity

Application

Expression System Type

Vector Map

Note

Caution

Shipping Condition

Lenti-SV40T  (No Selection Marker) Lentivirus, High Titer

Lenti-SV40T (No Selection Marker) Lentivirus, High Titer (LV665) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing SV40 large T antigen without a selection marker, supplied as 2 × 100 µl for primary cell immortalization, co-transduction, and custom selection workflows. Shipped on dry ice; store at –80°C.

Lenti-SV40T (Neo) Lentivirus, High Titer

Lenti-SV40T (Neo) Lentivirus, High Titer (LV660) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing SV40 large T antigen with neomycin (G418) resistance, supplied as 2 × 100 µl for stable primary cell immortalization with antibiotic selection. Shipped on dry ice; store at –80°C.

Lenti-SV40T (Puro) Lentivirus, High Titer

Lenti-SV40T (Puro) Lentivirus, High Titer (LV613) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing SV40 large T antigen with puromycin resistance, supplied as 2 × 100 µl for stable primary cell immortalization with puromycin selection. Shipped on dry ice; store at –80°C.

Lenti-SV40Tt Lentivirus, High Titer

Lenti-SV40Tt Lentivirus, High Titer (LV614) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus co-expressing SV40 large T and small t antigens (SV40Tt), supplied as 2 × 100 µl for stable immortalization of challenging primary cell types. Shipped on dry ice; store at –80°C.

<h2 dir="ltr"> What Are Lentiviral Cloning Vectors?</h2>
 The transfer plasmid is the heart of every lentiviral construct &mdash; it defines the transgene cargo, promoter driving expression, selection marker, and structural LTR elements that enable stable genomic integration. Selecting the right cloning vector backbone is the first and most consequential decision in any lentiviral project.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li style="font-family: arial, helvetica, sans-serif;">Expression vectors featuring CMV, EF1&alpha;, PGK, and UbC promoter configurations for transgene expression studies</li>
<li>Fluorescent reporter vectors incorporating GFP, RFP, and CFP for cell tracking and visualization</li>
<li>Promoterless vectors for promoter characterization and regulatory element analysis</li>
<li>HA-tag and His-tag expression vectors for protein detection and purification workflows</li>
<li>Bicistronic and tricistronic vector systems for multi-gene expression applications</li>
<li>miRNA- and siRNA-compatible cloning vectors for RNA interference studies</li>
<li>Functional vectors including selectable-marker and HSVtk-based constructs for specialized research applications</li>
</ul>
<h2 dir="ltr"> How to Choose a Lentiviral Cloning Vector</h2>
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Vector Design
Expression vectors, reporter vectors, promoterless systems, tagged constructs, and multicistronic backbones are designed for different experimental objectives. Vector selection should be guided by the intended application, transgene design, and downstream analysis requirements.
Promoter Selection
Promoters influence the strength and pattern of transgene expression. Available vector configurations include CMV, EF1&alpha;, PGK, and UbC promoters, as well as promoterless backbones for studies focused on promoter activity and regulatory elements.
Reporter Requirements
Fluorescent reporters such as GFP, RFP, and CFP enable visualization of transduced cells, monitoring of expression, and assessment of transduction efficiency. Reporter selection should align with the imaging platform and experimental workflow.
Expression Configuration
Single-gene, bicistronic, and tricistronic vector designs provide different levels of flexibility for co-expression studies. Multi-cassette systems can simplify experiments requiring coordinated expression of multiple genes or reporter elements.
Insert Size Considerations
Lentiviral vectors can accommodate relatively large expression cassettes compared with several other viral delivery systems. However, larger inserts may reduce viral production efficiency and should be evaluated during vector design and validation.
<h2 dir="ltr"> Specifications Context</h2>
 Modern research lentiviral vectors are commonly designed with self-inactivating (SIN) long terminal repeats (LTRs), an important biosafety feature that reduces promoter activity within the integrated viral LTR following transduction. Many lentiviral vector systems also incorporate regulatory elements that support efficient transgene expression and vector performance. Vector architecture, promoter selection, reporter configuration, and insert size can all influence downstream cloning, viral production, and expression outcomes. Product availability reflects MBP's catalogue as of mid-2026. 
The search for reliable lentiviral cloning vectors is over. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and find the perfect match today.

Cloning Vectors

Selection-Drug Killing Curve

Lenti-Tri-cistronic Vector

LV038 by Applied Biological Materials is a third-generation lentiviral vector (10 µg, Ice Pack) for simultaneous co-expression of three genes using a proprietary bidirectional promoter (~500 bp, two genes) + T2A self-cleaving peptide (third gene). Fits within the &lt;5.0 kb lentiviral packaging limit. Superior to IRES-based three-gene constructs in expression balance and insert size. Store at &minus;20°C.

Vector

<h2 dir="ltr">What Are miRNA Lentiviral Negative Controls?</h2>
 MicroRNAs (miRNAs) are approximately 22-nucleotide non-coding RNAs that regulate gene expression post-transcriptionally by binding to the 3&prime; UTR of target mRNAs, triggering mRNA degradation or translational repression via the RNA-induced silencing complex (RISC). Lentiviral delivery of miRNA expression constructs enables stable, long-term modulation of miRNA activity &mdash; essential where transient transfection approaches are insufficient.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Control Viruses models used in miRNA overexpression studies, for negative control normalization
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<li dir="ltr" aria-level="1">
Validation of 3'UTR target utilizing bioluminescent-based reporter assays.
</li>
<li dir="ltr" aria-level="1">
miRNA-Off Control Lentiviruses used to baseline microRNA knockdown effects.
</li>
<li dir="ltr" aria-level="1">
Blank 5&rsquo; UTR reporter vector optimized for gene expression normalization and translational regulation studies&nbsp;
</li>
<li dir="ltr" aria-level="1">
Reporter Stable Cell Lines models with Luciferase or GFP markers for reliable assay standardization.
</li>
</ul>
<h2 dir="ltr"> How to Choose a miRNA Negative Control</h2>
 Experiment Type Matching miRNA mimic (gain-of-function) experiments require a non-targeting miRNA mimic control. miRNA inhibitor/antagomiR (loss-of-function) experiments require a non-targeting inhibitor control. Lentiviral miRNA expression requires an empty vector or scrambled miRNA sequence. Mimic Control Design A non-targeting miRNA mimic control is a double-stranded RNA molecule resembling a mature miRNA duplex but with no significant homology to any known miRNA target in human, mouse, or rat transcriptomes &mdash; entering RISC the same way as a functional mimic. Inhibitor Control Design Non-targeting miRNA inhibitor (antagomiR) controls are single-stranded antisense sequences that enter the relevant cellular compartment without blocking any known miRNA, establishing the baseline for inhibitor-treated versus non-inhibitor comparisons. Vector Backbone for Lentiviral Delivery Stable lentiviral expression of miRNAs is used for long-term gain-of-function studies; the matched non-targeting lentiviral control must use the same promoter and vector backbone as the experimental construct to isolate sequence-specific effects. Inducible Format Availability Tet-On (doxycycline-responsive) non-targeting miRNA lentiviral controls are available for experiments requiring temporal control, used alongside inducible experimental constructs to control for doxycycline treatment itself. 
<h2 dir="ltr"> Specifications Context</h2>
 miRNA controls are designed to reflect the endogenous processing pathway of microRNAs, including Drosha and Dicer-mediated maturation before RISC loading. Because siRNA and miRNA constructs enter the RNA interference pathway differently, pathway-matched controls are important for accurate experimental interpretation. Non-targeting miRNA controls help distinguish sequence-specific effects from background effects associated with vector delivery and miRNA pathway activation. Available formats include lentiviral vectors, packaged viruses, reporter constructs, and stable cell line controls for miRNA and UTR-based studies. Product availability reflects MBP's catalogue as of mid-2026. 
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<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and request a quote for efficient miRNA control requirements.

miRNA Control

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

AAV Packaging Protocol

Lentivector Packaging Protocol

Lentivirus Infection Protocol

Lenti-UTR-GFP-Blank Virus

m015 by Applied Biological Materials is a pre-packaged Lenti-UTR-GFP-Blank control virus with no 3′ UTR insert. Establishes the no-3′UTR baseline GFP signal for lentiviral 3′ UTR miRNA reporter experiments. Ready to transduce. Store at −80°C; shipped on dry ice.

Titer

Storage Condition

Lenti-UTR-Luc-Blank Virus

m013 by Applied Biological Materials is a pre-packaged lentiviral firefly luciferase control virus produced from the pLenti-UTR-Luc-Blank (m012) backbone with an empty MCS — no 3'UTR insert. Supplied as 4 × 500 µl aliquots; no packaging required. Use as a matched transduction baseline control for m012-based miRNA 3'UTR reporter assays. Store at &minus;80°C; do not refreeze thawed aliquots.

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
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<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

Lenti-UbC-Blank Lentivirus

LVP689 by Applied Biological Materials is a pre-packaged UbC-blank lentiviral control virus supplied at 1×107 IU/mL as 200 µL. Empty-insert negative control for UbC-promoter lentiviral expression experiments. No reporter — confirm transduction by qPCR or parallel GFP control. Store at −80°C; shipped on dry ice.

Lenti-UbC-Blank-CBh-GFP-2A-Puro Control Vector

Lenti-UbC-Blank-CBh-GFP-2A-Puro Control Vector (Blank0622) by Applied Biological Materials is a 1.0 µg insert-empty lentiviral plasmid with UbC promoter backbone, CBh-EmGFP-2A-Puro cassette for transduction monitoring and puromycin selection — a matched negative control for UbC lentiviral expression experiments.

List of Markers

Lenti-UbC-Blank-CBh-RFP-2A-Puro Control Vector

Lenti-UbC-Blank-CBh-RFP-2A-Puro Control Vector Blank0623 (ABM) — 1.0 µg. Blank UbC lentiviral control with CBh-RFP-2A-Puro. Red fluorescence for transduction monitoring + puromycin selection. Use when experimental construct uses GFP to avoid spectral overlap. Store −20 °C. RUO.

Lenti-UbC-Blank-SV40-Blast Control Vector

Lenti-UbC-Blank-SV40-Blast Control Vector Blank0621 (ABM) — 1.0 µg. Blank UbC promoter lentiviral negative control with SV40-Blasticidin selection. Orthogonal to Hygro (Blank0620) and Neo (Blank0619) for multi-marker experiments. Store −20 °C. RUO.