Brands

<h2 dir="ltr"> What Are Lentiviral Cloning Vectors?</h2>
 The transfer plasmid is the heart of every lentiviral construct &mdash; it defines the transgene cargo, promoter driving expression, selection marker, and structural LTR elements that enable stable genomic integration. Selecting the right cloning vector backbone is the first and most consequential decision in any lentiviral project.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li style="font-family: arial, helvetica, sans-serif;">Expression vectors featuring CMV, EF1&alpha;, PGK, and UbC promoter configurations for transgene expression studies</li>
<li>Fluorescent reporter vectors incorporating GFP, RFP, and CFP for cell tracking and visualization</li>
<li>Promoterless vectors for promoter characterization and regulatory element analysis</li>
<li>HA-tag and His-tag expression vectors for protein detection and purification workflows</li>
<li>Bicistronic and tricistronic vector systems for multi-gene expression applications</li>
<li>miRNA- and siRNA-compatible cloning vectors for RNA interference studies</li>
<li>Functional vectors including selectable-marker and HSVtk-based constructs for specialized research applications</li>
</ul>
<h2 dir="ltr"> How to Choose a Lentiviral Cloning Vector</h2>
&nbsp;
Vector Design
Expression vectors, reporter vectors, promoterless systems, tagged constructs, and multicistronic backbones are designed for different experimental objectives. Vector selection should be guided by the intended application, transgene design, and downstream analysis requirements.
Promoter Selection
Promoters influence the strength and pattern of transgene expression. Available vector configurations include CMV, EF1&alpha;, PGK, and UbC promoters, as well as promoterless backbones for studies focused on promoter activity and regulatory elements.
Reporter Requirements
Fluorescent reporters such as GFP, RFP, and CFP enable visualization of transduced cells, monitoring of expression, and assessment of transduction efficiency. Reporter selection should align with the imaging platform and experimental workflow.
Expression Configuration
Single-gene, bicistronic, and tricistronic vector designs provide different levels of flexibility for co-expression studies. Multi-cassette systems can simplify experiments requiring coordinated expression of multiple genes or reporter elements.
Insert Size Considerations
Lentiviral vectors can accommodate relatively large expression cassettes compared with several other viral delivery systems. However, larger inserts may reduce viral production efficiency and should be evaluated during vector design and validation.
<h2 dir="ltr"> Specifications Context</h2>
 Modern research lentiviral vectors are commonly designed with self-inactivating (SIN) long terminal repeats (LTRs), an important biosafety feature that reduces promoter activity within the integrated viral LTR following transduction. Many lentiviral vector systems also incorporate regulatory elements that support efficient transgene expression and vector performance. Vector architecture, promoter selection, reporter configuration, and insert size can all influence downstream cloning, viral production, and expression outcomes. Product availability reflects MBP's catalogue as of mid-2026. 
The search for reliable lentiviral cloning vectors is over. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and find the perfect match today.

Cloning Vectors

Selection-Drug Killing Curve

Lenti-Bicistronic-Luc Vector

LV089 by Applied Biological Materials is the Lenti-Bicistronic-Luc Vector — 10 µg lentiviral plasmid for simultaneous dual-gene expression with luciferase. Proprietary ~500 bp IRES-free bidirectional bicistronic element — stronger downstream expression than IRES, smaller size. Requires packaging. Ice pack shipping; store at −20°C or below.

Unit Quantity

Vector

Note

Shipping Condition

<h2 data-section-id="xs82dy" data-start="121" data-end="167">What are additional immortalization agents?</h2>
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Additional immortalization agents cover recombinant lentiviral constructs delivering genes other than TERT or SV40 large T antigen used to bypass replicative senescence in primary cells, most notably HPV-16 E6/E7 (which inactivate p53 and Rb similarly to SV40T but are derived from human papillomavirus), Bmi1 (a polycomb group protein that represses the p16/CDKN2A senescence pathway), cMyc, CDK4, and HOXA/HOXB family genes. Related entities include p16/CDKN2A pathway repression (Bmi1), keratinocyte and epithelial cell immortalization (HPV E6/E7), polycomb group signaling, and combination immortalization strategies.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Lenti-HPV-16 E6/E7 degrades Rb and p53 for enhanced cell immortalization.</li>
<li dir="ltr" role="presentation">Lenti-Myc T58A &amp; Ras V12 enhance transformation and proliferation assays with high-titer mutant oncogenes&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-p53 &amp; Rb siRNA Virus extends cellular longevity and targets the knockdown of tumor suppressors</li>
<li dir="ltr" role="presentation">Lenti-HOX Series (RFP Tagged) for lineage-specific expansion and includes high-titer vectors like HOXB8, HOXA9, and HOXA10 vectors.</li>
<li dir="ltr" role="presentation">EBV Virus (Wild-Type) for immortalization workflows of B-lymphocytes&nbsp;</li>
<li dir="ltr" role="presentation">Lenti-CDK4 &amp; Bmi1 Virus helps maintain proliferative capacity using cell mediators</li>
</ul>
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<h2 data-section-id="1acs4rs" data-start="1029" data-end="1087">How to choose additional immortalization agent products</h2>
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HPV E6/E7 for epithelial and keratinocyte immortalization
HPV-16 E6 and E7 proteins inactivate p53 and Rb respectively, similar in net effect to SV40 large T antigen but derived from a different viral source; E6/E7-based immortalization is particularly well-documented for keratinocytes and other epithelial cell types, and is sometimes preferred over SV40T for these lineages based on published precedent.
Bmi1 for stem and progenitor cell types
Bmi1 represses the p16/CDKN2A senescence pathway through its role in the polycomb repressive complex, and has been used to extend the proliferative capacity of hematopoietic and neural stem/progenitor cells in research settings; confirm the specific cell type has documented precedent for Bmi1-based extension before relying on it as a primary immortalization strategy.
cMyc and CDK4 as proliferation-driving factors
cMyc and CDK4 overexpression can drive proliferation and contribute to immortalization, often used in combination rather than as standalone immortalization agents; review the specific combination strategy in the source literature before ordering individual components.
E6/E7 separate vs. fused constructs
HPV E6/E7 may be delivered as separate genes or as a fused open reading frame; confirm which configuration a published protocol specifies, since this can affect relative expression levels of the two proteins.
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<h2 data-section-id="1fljvas" data-start="3287" data-end="3312">Specifications context</h2>
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Additional immortalization agent lentiviruses are typically supplied at titers around 1x10^8 TU/mL, shipped on dry ice and stored at -80&deg;C, with antibiotic or fluorescent-antibiotic fusion selection markers consistent with other immortalization lentivirus products. Pack sizes for academic labs commonly consist of small-volume aliquots sufficient for multiple transduction attempts, with constructs validated by sequencing and tested free of common contaminants.&nbsp;
This sub-category sits within MBP's cell immortalization catalog alongside TERT overexpression viruses and SV40T antigen overexpression viruses. Labs constructing custom combination vectors can also review MBP's cloning reagents. MBP's specialist team can help confirm which immortalization agent has documented precedent for a specific cell type before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to book high-quality immortalization vectors for your lab.

Additional Immortalization Agents

MSDS

Lentiviral Vector Amplification - Protocol

Lentivector Packaging - Protocol

Lentivirus Infection - Protocol

Cell Immortalization - Handbook

Cell Immortalization Reagents - FAQ

Lenti-Bmi1 Virus, High Titer

Lenti-Bmi1 Virus, High Titer (LV608) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing Bmi1, supplied as 2 × 100 µl for immortalization of primary epithelial cells via PRC1-mediated repression of the INK4a/ARF locus, bypassing p16/pRb and p14/p53 senescence pathways. Shipped on dry ice; store at –80°C.

Application

Expression System Type

Vector Map

Caution

Lenti-CDK4 Virus, High Titer

Lenti-CDK4 Virus, High Titer (LV609) by Applied Biological Materials is a 10⁹ IU/ml recombinant lentivirus expressing CDK4, supplied as 2 × 100 µl for stable cell immortalization via CDK4-mediated pRb hyperphosphorylation and G1/S checkpoint bypass. Shipped on dry ice; store at –80°C.

<h2 dir="ltr">What Are Lentiviral Control &amp; Reporter Constructs?</h2>
 Rigorous experimental design requires matched controls that enter the same delivery workflow as the test construct. Lentiviral control and reporter constructs use the same vector backbones, promoters, and packaging systems as experimental vectors &mdash; ensuring observed differences between treated and control cells reflect genuine biological effects rather than artefacts of the delivery process.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1"><a href="https://mbpinc.net/reagents/dna/cloning-services-products/lentivirus/over-expression/control/reporter/luciferase-reporter">Luciferase Reporters: </a>for reliable assay validation using selective promoter-specific vectors, fluorescent reporters, dual-reporter systems, and background control</li>
<li data-section-id="644n3l" data-start="108" data-end="253">Fluorescent Reporter Controls: GFP and RFP lentiviral vectors and viruses for monitoring transduction efficiency and transgene expression.</li>
<li data-section-id="19809zr" data-start="255" data-end="399">Blank Control Vectors &amp; Viruses: Empty lentiviral backbones for negative controls, promoter evaluation, and custom construct development.</li>
<li data-section-id="112pumm" data-start="401" data-end="569">Promoter &amp; UTR Reporter Systems:&nbsp; Reporter vectors designed for promoter activity and regulatory element analysis, including promoter and 5&prime; UTR reporter formats.</li>
<li data-section-id="1j5a9h3" data-start="571" data-end="736">Selectable Marker Controls: Control constructs with puromycin, neomycin, hygromycin, and blasticidin resistance markers for selection and workflow validation.</li>
</ul>
<h2 dir="ltr"> How to Choose Control &amp; Reporter Constructs</h2>
 Control Type Needed Empty vector controls carry only the selection marker (puromycin, blasticidin, or hygromycin resistance) without a gene of interest, providing the baseline reference for overexpression or knockdown experiments. Fluorescent Reporter Channel GFP and RFP lentiviral reporters allow real-time monitoring of transduction efficiency by flow cytometry or fluorescence microscopy, matching the experimental construct's promoter for an accurate expected-expression reference. Luciferase Reporter Pairing Firefly luciferase (P. pyralis) and Renilla luciferase (R. reniformis) are the two most commonly paired reporters; because they use different substrates (D-luciferin and coelenterazine, respectively), both signals can be measured sequentially from the same sample. Promoter-less Baseline A promoter-less dual luciferase control vector provides a no-promoter baseline against which promoter-driven reporter constructs are normalised &mdash; useful for establishing background signal levels. Vector Backbone Matching Using a matched vector backbone ensures observed differences between experimental and control cells are due to the transgene, not differences in vector, promoter strength, integration preference, or selection marker. 
<h2 dir="ltr"> Specifications Context</h2>
 Because lentiviral integration is heritable, transduced cells maintain stable reporter gene expression across cell divisions &mdash; stable luciferase or GFP reporter cell lines generated this way are widely used in drug screening, pathway activation assays, and long-term in vivo imaging. Through ABM, MBP can supply promoter-reporter lentiviral constructs covering human and mouse promoters, driving firefly luciferase or GFP expression, as plasmid DNA or pre-packaged particles. Both plasmid and pre-packaged formats are available for immediate dispatch, with institutional account pricing available for core facilities. Core facilities managing reporter constructs across multiple research groups often standardise on a small set of validated control vectors to simplify inventory and ensure consistency across projects.&nbsp; 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get high-quality lentiviral control/reporters for your lab.

Control/Reporter

AAV Packaging Protocol

Lentivirus Infection Protocol

Lentiviral Vector Amplification Protocol

AAV Infection Guideline

Lentivector Packaging Protocol

Lenti-CMV-Blank-SV40-Blast Control Vector

Lenti-CMV-Blank-SV40-Blast Control Vector (Blank0603) by Applied Biological Materials is a 1.0 µg insert-empty lentiviral plasmid with CMV promoter backbone and SV40-Blast (blasticidin) selection cassette, for use as a matched negative control in CMV-promoter lentiviral gene expression experiments. Store at –20°C or below.

List of Markers

Lenti-CMV-Blank-SV40-Hygro Control Vector

Lenti-CMV-Blank-SV40-Hygro Control Vector (Blank0602) by Applied Biological Materials is a 1.0 µg insert-empty lentiviral plasmid with CMV promoter backbone and SV40-Hygro (hygromycin) selection cassette, for use as a matched negative control in CMV-promoter lentiviral gene expression experiments. Store at –20°C or below.

System

Promoter

Lenti-CMV-Blank-SV40-Neo Control Vector

Lenti-CMV-Blank-SV40-Neo Control Vector (Blank0601) by Applied Biological Materials is a 1.0 µg insert-empty lentiviral plasmid with CMV promoter backbone and SV40-Neo selection cassette, for use as a matched negative control in CMV-promoter lentiviral gene expression experiments. Store at –20°C or below.

<h2 dir="ltr">What Are Cre-Inducible DIO Lentiviral Vectors?</h2>
 Conditional gene expression systems restrict transgene activation to defined cell populations &mdash; indispensable when whole-tissue expression would confound experimental interpretation or produce toxicity. DIO (Double-floxed Inverted Open reading frame) architectures place the gene of interest in an inverted, non-coding orientation until Cre recombinase flips it into the correct sense orientation for transcription.&nbsp;Conditional expression studies require precise genetic control. Choose a DIO vector compatible with your experimental model.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li>Lenti-CMV-DIO-copGFP-SV40-Puro Vectors: Feature copGFP fluorescent tracking and a puromycin resistance marker for the selection of stable cells.</li>
<li>Blank DIO-SV40-copGFP Vectors: Feature a copGFP reporter designed for cell-specific gene insertion and expression.</li>
<li>Lenti-CMV-DIO-Blank-SV40-Puro Vectors: Feature an empty DIO expression plasmid with SV40-driven puromycin resistance for reliable experimental normalization.</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a DIO Lentiviral Vector</h2>
 Insert Size AAV DIO vectors are limited to expression cassettes of approximately 4.2 kb. Lentiviral DIO vectors support cassettes up to ~10 kb, enabling delivery of large genes &mdash; including full-length membrane receptors, ion channels, and fluorescent fusion constructs &mdash; that cannot fit in an AAV genome. Cre Delivery Method Cre can be delivered by co-injection of a Cre-expressing lentiviral or AAV vector, stereotactic injection of DIO LV into a transgenic Cre-driver mouse line, or plasmid co-transfection of Cre in cell culture experiments. Permanence of Activation Once Cre flips the ORF into sense orientation, one site from each loxP pair is excised, leaving heterotypic lox sites that cannot undergo further Cre-mediated recombination &mdash; the transgene is permanently locked ON and heritably expressed in daughter cells. Validation Before In Vivo Use Co-transfection of the DIO vector with a Cre expression plasmid in HEK293T cells confirms efficient Cre-mediated inversion and expected transgene expression. Transfecting the DIO vector alone confirms the absence of leaky expression. Reporter and Application Format DIO lentiviral vectors are available with GFP, mCherry, YFP, and other fluorescent reporters &mdash; as the primary transgene or fused via a 2A self-cleaving peptide for simultaneous labelling and functional expression. Optogenetic actuators (ChR2, eNpHR, Arch) and chemogenetic tools (DREADDs) are common applications. 
<h2 dir="ltr"> Specifications Context</h2>
 DIO (Double-floxed Inverted Orientation) lentiviral vectors enable Cre-dependent gene expression using recombination-based activation of inverted transgene cassettes. These systems are commonly used in conditional expression workflows involving fluorescent reporters, selectable markers, and controlled gene expression models. A known technical consideration is baseline leakiness before Cre recombination, which can vary depending on vector design and experimental conditions. MBP&rsquo;s DIO lentiviral vector offerings include blank, GFP, and selection-marker formats designed to support a range of Cre-dependent experimental setups. Product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get a reliable Cre-inducible gene expression (DIO) vector for your lab.

Cre-Inducible Gene Expression (DIO) Vectors

Vector Brochure

AAV–General Guideline to Serotype Selection

AAV-Infection Guideline

Lentivirus-Infection Guideline

Lenti-CMV-DIO-Blank-SV40-Puro Vector

Lenti-CMV-DIO-Blank-SV40-Puro Control Vector (LV662) by Applied Biological Materials is a 1.0 µg insert-empty Cre-inducible DIO lentiviral plasmid with CMV promoter and SV40-puromycin selection cassette, for use as a negative control in Cre-On conditional expression experiments. Store at –20°C or below.

Lenti-CMV-DIO-Blank-SV40-copGFP Vector

Lenti-CMV-DIO-Blank-SV40-copGFP Control Vector (LV663) by Applied Biological Materials is a 1.0 µg insert-empty Cre-inducible DIO lentiviral plasmid with CMV promoter and SV40-copGFP fluorescence reporter — matched negative control for Cre-On DIO expression experiments with fluorescence-based transduction monitoring. Store at –20°C or below.

Lenti-CMV-DIO-copGFP-SV40-Puro Vector

Lenti-CMV-DIO-copGFP-SV40-Puro Control Vector (LV661) by Applied Biological Materials is a 1.0 µg Cre-inducible lentiviral DIO plasmid with CMV-copGFP (Cre-activated) and SV40-puromycin (constitutive), for Cre activity reporting with puromycin selection in lentiviral DIO experiments. Lentiviral counterpart to AAV102. Store at –20°C or below.

QC-LV073 (Lot No. SY7947)

Lenti-CMV-GFP-2A-Puro Vector

LV073 by Applied Biological Materials is a third-generation lentiviral transfer vector (10 µg) co-expressing GFP and Puromycin resistance via a T2A self-cleaving peptide from a single CMV-driven transcript. T2A ribosomal skipping produces two separate proteins — no GFP-Puro fusion. For simultaneous transduction tracking and Puromycin-based stable cell line generation. Store at &minus;20°C.

Lenti-CMV-GFP-2A-Puro-Blank Lentivirus

LVP690 by Applied Biological Materials is a pre-packaged CMV/CBH-GFP-2A-Puro blank lentiviral control virus. No transgene; GFP + Puromycin resistance via 2A peptide. Supplied at 108 IU/mL as 3 × 250 µL. Matched negative control for CMV-CBH-GFP-2A-Puro backbone experiments. Ready to transduce. Store at −80°C; shipped on dry ice.

Titer

Lenti-CMV-RFP-2A-Puro-Blank Lentivirus

LVP691 by Applied Biological Materials is a pre-packaged CMV-mKate2-2A-Puro blank lentiviral control virus. mKate2 far-red RFP (∼633 nm) + Puromycin resistance via 2A peptide. Supplied as 4 × 500 µL. Matched negative control for CMV-RFP-2A-Puro backbone experiments. Ready to transduce. Store at −80°C; shipped on dry ice.