Brands

<h2>What Are Dual Baths?</h2>
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Dual temperature baths are instruments with two physically separate heating (or heating and cooling) zones within a single chassis, each zone individually controlled by a dedicated microprocessor thermostat or PID controller with its own setpoint, timer display, and alarm system. The most common format is a dual-zone dry block incubator (metal bath): two aluminum heating blocks with interchangeable well inserts, one block capable of heating-only (ambient to 100 degrees C) and the other capable of heating and cooling (-10 to 100 degrees C using Peltier semiconductor cooling). Water-format dual baths also exist, with two separate compartments and heating elements, but the dry block format is more common for dual-zone applications because the zones can be physically adjacent without risk of water mixing between zones. Use dual baths to run two reactions at different temperatures simultaneously on a single footprint, for example, 37 degrees C enzyme digestion and 70 degrees C heat inactivation, or a ligation at 16 degrees C while a second protocol runs at 37 degrees C.
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<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<ul>
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Julabo Thermal Bath Fluids: Optimized 5L and 10L solutions ideally suited for all temperature control applications to guarantee safe and reliable operation.
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Superior Performance Benefits: High-stability fluids featuring low toxicity, minimum odor, and low viscosity for efficient heat transfer and reduced corrosion.
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Wide Temperature Ranges: Technical-grade fluids capable of working from 0&deg;C to +220&deg;C, with high flash points over +232&deg;C for enhanced laboratory safety.
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Instrument Optimization: Specifically selected fluids matched to temperature control units to ensure ideal oxidation characteristics and thermal performance.
</li>
</ul>
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<h2>How to Choose a Dual Bath</h2>
&nbsp;
Heating-only vs. heating-and-cooling zones
The simplest dual baths have two heating-only zones, each operating from ambient to 100 degrees C, suited for workflows needing simultaneous incubation at two temperatures above room temperature, such as 37 degrees C and 55 degrees C running in parallel. More versatile dual bath models have one heating-and-cooling zone (using Peltier semiconductor technology) operating from -10 to 100 degrees C alongside a second heating-only zone, enabling protocols that require a subambient setpoint on one block (ice-cold buffer preparation, 4 degrees C sample holding) while running a warm incubation simultaneously.
Block format and tube compatibility
Dual dry block baths use interchangeable aluminum well inserts that accommodate specific tube formats: 0.5 mL PCR tubes, 1.5 mL and 2 mL microcentrifuge tubes, 0.2 mL PCR strip tubes, 15 mL conical tubes, or glass reaction vials. Blocks with adjustable or multi-format inserts provide greater flexibility. Confirm that inserts for your specific tube formats are included with the instrument or available separately before ordering. Some models provide a flat heating surface insert suitable for microplates or irregular sample formats.
Independent control and display
Each zone must have a truly independent controller: the temperature, timer, and alarm settings for Zone A must have no effect on Zone B. Models with a single combined controller that switches between zones are less convenient than those with dedicated display panels per zone. Confirm that the simultaneous dual-display interface shows the actual and setpoint temperatures for both zones simultaneously without requiring mode switching.
Temperature stability and accuracy
Dual block dry bath incubators typically achieve temperature stability of plus or minus 0.2-0.5 degrees C per zone, with accuracy of plus or minus 0.3 degrees C at 37 degrees C (as specified for DLAB models with PT1000 sensors). Built-in calibration function and automatic fault detection with audible alarm are standard features on current research-grade models. An adjustable heated lid (covering the tops of tubes to prevent condensation) is included on some models, useful for reactions with volatile components at elevated temperatures.
Cooling zone specifications
When one zone includes Peltier cooling, the minimum temperature achievable is typically -10 degrees C with a cooling rate of approximately 1-3 degrees C per minute from ambient; this is distinct from active refrigeration (compressor-based) and is slower than a refrigerator. Peltier cooling is energy-efficient for moderate sub-ambient temperatures; for temperatures below -10 degrees C, a separate compressor-refrigerated dry bath is required.
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<h2>Specifications Context</h2>
&nbsp;
Dual dry block incubators typically draw 200-400 W total power at 120 V for two heating-only zones; adding a Peltier cooling zone increases power consumption modestly due to the semiconductor heat pump. Auto-restart after power failure, over-temperature protection at a preset safety threshold, and built-in temperature calibration function are standard specifications on current dual bath models. Block heating is significantly faster to setpoint than water baths: most dual dry block units reach 37 degrees C from ambient in 3-5 minutes, compared to 10-15 minutes for a 5-10 L water bath, making them the preferred format when frequent setpoint changes between protocols are needed. Dual dry block incubators with Peltier cooling, LED or LCD dual displays, and auto-recognized interchangeable blocks have replaced single-zone dry baths in many molecular biology labs requiring simultaneous two-temperature workflows.
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To discuss product availability or get selection guidance,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.

Dual Baths

Julabo C20S +220°C Thermal Bath Fluid - 10L

C20S-10L Standard Package

Julabo P90 -90°C to +170°C Thermal Bath Fluid - 10L

P90-10L Standard Package

Julabo EG -30°C to +80°C Thermal Bath Fluid - 10L

EG-10L  Standard Package

Julabo Thermal Bath Fluid – 10L

Julabo thermal bath fluids in 10 L cans: C20S (up to +220°C, C20S-10L), P90 (−90°C to +170°C, P90-10L), and EG ethylene glycol (−30°C to +80°C, EG-10L). Formulated for Julabo temperature control instruments.

Manufacturer

Julabo C20S +220Â°C Thermal Bath Fluid - 10L

Julabo P90 -90Â°C to +170Â°C Thermal Bath Fluid - 10L

Julabo EG -30Â°C to +80Â°C Thermal Bath Fluid - 10L

<h2 dir="ltr">What Are Specialized Cloning Vectors &amp; Viral Tools?</h2>
 Standard lentiviral and retroviral expression vectors meet the needs of most overexpression and knockdown workflows, but many cell biology and translational research questions demand purpose-engineered constructs with specialised functions &mdash; subcellular localisation, multi-gene co-expression, or conditional activation. Select the format best suited to your experimental requirements.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
<a href="https://mbpinc.net/reagents/dna/cloning-services-products/specialized-cloning-vectors/-virus/organelle-labeling-viruses">Organelle Labeling Viruses: </a>for live-cell visualization and functional pathway analysis of cellular structures in mammalian systems
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Targeted fluorescent labeling of specific cellular structures using viral delivery.
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Stable expression of organelle-specific markers for long-term live-cell imaging.
</li>
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Compatible with fluorescence microscopy for dynamic cellular imaging applications.
</li>
<li data-section-id="8k7pm2" data-start="506" data-end="603" data-is-last-node="">Suitable for studying organelle organization, transport, and cellular function in living cells.</li>
</ul>
<h2 dir="ltr"> How to Choose Specialized Cloning Vectors</h2>
 Subcellular Targeting Needs Organelle-targeted fluorescent labeling lentiviral particles deliver fluorescent protein constructs targeting mitochondria, endoplasmic reticulum, nucleus, Golgi, lysosome, peroxisome, plasma membrane, autophagosome, and cytoskeleton &mdash; providing a stable alternative to chemical dyes for live-cell imaging. Multi-Gene Co-Expression Bicistronic vectors using IRES or 2A self-cleaving peptide sequences enable co-expression of two genes (e.g., a gene of interest and a fluorescent marker) from a single transcript or vector. Conditional Expression Requirements Conditional DIO/FLEX vectors restrict transgene expression to Cre-expressing cells, essential for circuit-specific neuroscience research and in vivo conditional gene expression studies. Temporal Control Needs Inducible constructs (Tet-On/Tet-Off systems) provide doxycycline-regulated transgene activation for experiments requiring temporal control over expression timing. Delivery Format Most specialised constructs are available as lentiviral particles for stable, long-term expression in both dividing and non-dividing cell types, including primary cells and in vivo models. 
<h2 dir="ltr"> Specifications Context</h2>
&nbsp;
Traditional subcellular organelle imaging relies on chemical dyes (e.g., MitoTracker, LysoTracker, ER-Tracker) or antibody-based immunofluorescence in fixed cells; however, chemical dyes are transient and may exhibit cytotoxicity at working concentrations, while antibody-based methods are limited to endpoint imaging. Viral organelle-targeted fluorescent expression systems enable stable, genetically encoded labeling for long-term live-cell imaging and dynamic subcellular tracking. These constructs are supplied in plasmid DNA and pre-packaged viral formats and support applications including organelle dynamics, trafficking studies, and multicolor imaging workflows. Specialized cloning vectors and viral tools are sourced from established manufacturers, including Applied Biological Materials (ABM), and product availability reflects MBP&rsquo;s catalogue as of mid-2026.
&nbsp;
<a href="https://mbpinc.net/contact-us" style="color: #236fa1;">Contact the MBP team </a>today and get specialized cloning vectors for your lab.

Specialized Cloning Vectors/ Viruses

Specialized Cloning Vectors/ Virus

KJK Lenti-blast-GCaMP6s

AX0010023 by Applied Biological Materials is a KJK Lenti-blast lentiviral transfer plasmid (1.0 µg) encoding GCaMP6s (high-sensitivity slow green GECI; excitation ~494 nm, emission ~514 nm) with Blasticidin selection. For stable, lentivirally delivered GCaMP6s expression in mammalian cells. Enables detection of low-amplitude calcium transients including single action potentials. Blasticidin marker compatible with Puromycin or Neomycin dual-selection constructs.

Unit Quantity

Storage Condition

Shipping Condition

<h2 data-section-id="1go10h8" data-start="173" data-end="215">What are classic cell culture media?</h2>
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Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture since the mid-20th century: DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's Nutrient Mixtures F-10 and F-12, IMDM (Iscove's Modified Dulbecco's Medium), and the combination formulation DMEM/F-12. Each is manufactured according to its originally published formula, with modifications limited to those necessary for consistent performance and stability of the medium over its shelf life. These media supply the amino acids, vitamins, glucose, salts, and (in most formulations) phenol red pH indicator that form the nutritional backbone of essentially all subsequent specialty, serum-free, and Advanced media derivatives.
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<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Selective Mammalian Antibiotics including Puromycin, Geneticin, and Hygromycin B for stable development of cell lines.</li>
<li dir="ltr" role="presentation">Broad-Spectrum Bactericidal Agents for routine contamination prevention, including Penicillin/Streptomycin and Gentamicin&nbsp;</li>
<li dir="ltr" role="presentation">Prokaryotic Selection Reagents for effective bacterial transformation, including Ampicillin, Kanamycin, Chloramphenicol, and Tetracycline&nbsp;</li>
<li dir="ltr" role="presentation">Antifungal and Antimycotic Solutions to maintain sterile cultures, including combined 100X cocktails and Amphotericin B (Fungizone)&nbsp;</li>
<li dir="ltr" role="presentation">Specialized Small Molecules for targeted biochemical assays, including Ganciclovir, Rapamycin, and Actinomycin D&nbsp;</li>
</ul>
&nbsp;
<h2 data-section-id="1mfajxk" data-start="1218" data-end="1252">How to choose a classic medium</h2>
&nbsp;
DMEM vs MEM
DMEM contains up to four times the amino acid and vitamin concentration of original Eagle's MEM, along with higher glucose levels, making it suitable for cell types with elevated nutrient demands &mdash; DMEM is broadly applicable across mammalian, insect, and plant cell phenotypes including primary cells, stem cells, and transformed lines. MEM remains widely used for monolayer culture of many standard cell types, with optional Hanks' or Earle's salt formulations and a calcium-free modification that permits suspension growth.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640, developed at the Roswell Park Memorial Institute for culturing human leukemic cells, is optimized for non-adherent cell types &mdash; lymphocytes and other blood cells &mdash; and contains glutathione plus higher biotin, B12, and PABA concentrations than DMEM or MEM. Despite its hematopoietic origin, RPMI 1640 supports a broad range of adherent lines (HeLa, T47D, RENCA, CT26.WT) when properly supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were originally developed for CHO cell clones, HeLa cells, and mouse L-cells, and remain suited to hepatocyte culture, viral fusion experiments, and toxicity assays, with formulations available for use with or without serum. Coon's modification of Ham's F-12 roughly doubles the amino acid and pyruvate content and adds ascorbic acid, developed specifically for hybrid cells produced by viral fusion.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 (1:1 mixture) combines DMEM's glucose, amino acid, and vitamin richness with Ham's F-12's zinc, putrescine, hypoxanthine, and thymidine, producing an extremely rich complex medium used for MDCK cells, glial cells, fibroblasts, and human endothelial cells &mdash; and serving as the basal medium for many serum-free Advanced formulations used in organoid and stem cell work.
IMDM for high-density rapid proliferation
IMDM further enriches DMEM with additional amino acids and vitamins, substituting potassium nitrate for ferric nitrate and adding HEPES and sodium pyruvate. IMDM's sodium bicarbonate buffer system (5&ndash;10% CO₂) and enriched composition make it well suited for rapidly proliferating, high-density cultures including Jurkat, COS-7, and macrophage cells.
Liquid vs. powder format
Liquid classic media are ready to use (after appropriate FBS and supplement addition) and include sodium bicarbonate in the formulation. Powdered media are manufactured without sodium bicarbonate, since it tends to off-gas in powder form &mdash; 3.7 g/L sodium bicarbonate must be added upon reconstitution, followed by sterile filtration. Powder formats offer cost and shipping-weight advantages for high-volume labs preparing their own media.
&nbsp;
<h2 data-section-id="17i0pj" data-start="5035" data-end="5061">Specifications context</h2>
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Classic media are manufactured according to originally published formulas, with stability-related modifications documented per product. DMEM's sodium bicarbonate buffer system at 3.7 g/L is standard across liquid formulations; powdered formats require this addition upon reconstitution along with 0.22 &micro;m sterile filtration. As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the three most widely specified classic media across academic and biomanufacturing labs, with IMDM increasingly used for high-density suspension applications in bioprocessing.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents (GlutaMAX, NEAA, sodium pyruvate, HEPES), see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get highly efficient microbial control solutions for your lab.

Antibiotics

MSDS

Kanamycin

G022 Kanamycin 100 mg/ml by ABM — aminoglycoside antibiotic from S. kanamyceticus; 30S ribosomal subunit inhibitor for KanR bacterial clone selection; 5×1.0 ml; store at −20°C. For plasmid maintenance and bacterial genetic selection.

Concentration

Shipping condition

Kanamycin Sulfate

A1003-ZYM by Zymo Research is a ready-to-use Kanamycin Sulfate solution at 35 mg/ml (≥98% purity), sterile-filtered through a 0.2 µm membrane for direct use in E. coli selection and plasmid amplification. Working concentration: 30 µg/ml in standard bacterial media. Store at -20°C. Available in 1×5 ml or 5×5 ml formats. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

Product Storage

Purity

Working Concentration

Kanamycin Sulfate (35 mg/ml) (5 x 5 ml)

Kanamycin Sulfate (35 mg/ml) (5 ml)

<h2 data-section-id="1h8mvlk" data-start="0" data-end="45">What are primary cell culture medium kits?</h2>
&nbsp;
Primary cell culture medium kits are complete, ready-to-prepare culture systems that combine a cell-type-specific basal medium with the supplements required for optimal growth and maintenance of primary cells. Unlike purchasing media, serum, growth factors, and antibiotics separately, these kits provide all components in validated proportions, ensuring that the medium performs as intended for a specific primary cell type.
A typical kit includes a basal medium, a cell-specific growth supplement, an antibiotic component where appropriate, and fetal bovine serum (FBS) or an equivalent supplement when required. The components are designed to be combined directly according to the manufacturer's instructions, reducing preparation errors and improving consistency between experiments.
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<h2 data-path-to-node="5">What you will find:</h2>
&nbsp;
<ul data-path-to-node="6">
<li style="font-family: arial, helvetica, sans-serif;">
Tissue-specific primary cell culture medium kits
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Specialized growth media for endothelial and epithelial cells
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<li style="font-family: arial, helvetica, sans-serif;">
Workflow systems for thawing, plating, and maintenance of hepatocytes&nbsp;
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Differentiation media for progenitor and stem cells&nbsp;
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Serum-free bioprocessing and cryopreservation reagents
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Targeted fibroblast, smooth muscle, and myocyte growth kits
</li>
</ul>
&nbsp;
<h2 data-section-id="1nip7jz" data-start="1066" data-end="1116">How to choose a primary cell culture medium kit</h2>
&nbsp;
Match the kit to the cell type
Primary cells have highly specialized nutritional and signaling requirements. Kits are available for endothelial cells, hepatocytes, astrocytes, epithelial cells, fibroblasts, stem cells, and many other cell types. Selecting a kit specifically designed for the target cell type is essential for maintaining normal cellular function and morphology.
Serum-containing vs. serum-free formulations
Traditional kits often include FBS as a source of growth factors and nutrients. Serum-free and xeno-free kits replace animal-derived components with defined supplements, reducing variability and supporting applications that require animal-component-free culture conditions.
Complete kit convenience
Most kits provide all major components needed to prepare complete culture medium. This eliminates the need to source multiple reagents individually and helps ensure that supplements are used at the correct concentrations.
Antibiotic inclusion
Many primary cell culture kits include antibiotics to reduce contamination risk during routine culture. However, some specialized formulations, particularly those designed for immune cells and mononuclear cells, are intentionally supplied without antibiotics because these additives can influence cellular responses and experimental outcomes.
Storage and preparation
Kit components are typically stored separately until needed. Basal media and supplements are usually refrigerated, while serum components may require frozen storage before use. Once mixed into complete medium, shelf life is generally shorter than that of the individual components, making it advisable to prepare only the amount needed for near-term use.
Specialized differentiation systems
Some kits are designed not only for maintenance but also for cellular differentiation. These systems may include multiple supplements added sequentially throughout a protocol to guide stem cells or progenitor cells through defined developmental stages.
<h2 data-section-id="dydsco" data-start="3131" data-end="3173">&nbsp;</h2>
<h2 data-section-id="1fljvas" data-start="4061" data-end="4086">Specifications context</h2>
&nbsp;
Primary cell culture medium kits are commonly supplied in a format consisting of a basal medium bottle accompanied by concentrated supplements and serum components that are added directly before use. This approach simplifies preparation while ensuring that all components have been optimized and tested together.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at MBP">Contact the expert team at MBP</a> today and get optimized primary cell culture medium kits for your lab.

Primary Cell Culture Medium kits

Flyer

Keratinocyte Medium Kit

Keratinocyte Medium Kit (TM040) by Applied Biological Materials (ABM) includes 500 mL basal medium and keratinocyte-specific supplements, QC tested for sterility, pH, osmolality, and endotoxin. Requires 2% FBS (user-supplied). Store basal medium at 4–8°C; supplements at −20°C. Use within 30 days of supplementation.

Sterility

Appearance

Disclaimer

Kingfisher Plastics

<h2 data-section-id="144nuix" data-start="0" data-end="40">What Are KingFisher Flex Consumables?</h2>
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KingFisher Flex consumables are specialized plates, tip combs, and accessories designed specifically for Thermo Scientific KingFisher magnetic particle processors, including the KingFisher Flex, Apex, Duo Prime, and Presto systems. These consumables are engineered to support automated magnetic bead-based purification workflows for DNA extraction, RNA isolation, protein purification, cell isolation, and other molecular biology applications. Their precise dimensions and well geometries ensure accurate magnetic rod movement, efficient bead transfer, and consistent purification results across high-throughput laboratory workflows.
Unlike standard deep well plates, KingFisher consumables are manufactured from low-binding polypropylene to minimize the loss of nucleic acids and proteins during extraction and washing steps. This helps maximize sample recovery, improve purity, and maintain reproducibility in research, clinical diagnostics, biobanking, and next-generation sequencing (NGS) applications.
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<h2 dir="ltr">At MBP, You can discover:</h2>
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<ul>
<li dir="ltr" aria-level="1">
6 Deep-Well Plates for KingFisher Flex and Presto systems
</li>
<li dir="ltr" aria-level="1">
24-Well Deep Well Plates for high-volume extraction
</li>
<li dir="ltr" aria-level="1">
96-Format Plastic Tip Combs for magnetic bead collection
</li>
<li dir="ltr" aria-level="1">
U-bottom Square Well Plates for minimal volume
</li>
<li dir="ltr" aria-level="1">
10.4 ml 24-well plates for large-scale purification
</li>
<li dir="ltr" aria-level="1">
Medical-grade Polypropylene Plates for chemical stability
</li>
<li dir="ltr" aria-level="1">
Sterile, DNase/RNase-free consumables for sensitive assays
</li>
<li dir="ltr" aria-level="1">
High-recovery extraction plates for maximum specimen yield
</li>
</ul>
&nbsp;
<h2 data-section-id="1h779p8" data-start="1262" data-end="1306">How to Choose KingFisher Flex Consumables</h2>
&nbsp;
Instrument Configuration
Consumable selection depends on the instrument model and processing head installed. Most KingFisher Flex systems use a 96-well head designed for medium-throughput sample processing, while certain applications require a 24-well head for larger sample volumes. Always verify your instrument configuration before ordering consumables.
Sample Volume Requirements
The 96-well format is ideal for routine DNA and RNA extraction from blood, plasma, swabs, tissue lysates, and cultured cells. Applications requiring larger input volumes, such as circulating cell-free DNA isolation, environmental testing, or stool sample processing, often use the 24-well format, which accommodates significantly larger volumes per well.
Sterility Requirements
Non-sterile consumables are commonly used for standard nucleic acid purification and protein extraction workflows. Sterile plates and sterile plate-tip comb combination packs are recommended for cell-based applications, sensitive RNA workflows, and any procedure requiring aseptic processing conditions.
Individual Components vs. Combination Packs
Many laboratories purchase plates and tip combs separately to match workflow requirements. Combination packs containing both sterile plates and matching sterile tip combs simplify inventory management and ensure compatibility between components.
Downstream Application
For PCR, qPCR, sequencing, and library preparation workflows, purified samples are typically transferred into 96-well microplates with smaller working volumes. Applications requiring larger elution volumes may use deep well plates for the final collection step.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3463" data-end="3488">Specifications Context</h2>
&nbsp;
KingFisher consumables are manufactured from high-purity, low-binding polypropylene to reduce non-specific adsorption of DNA, RNA, proteins, and magnetic beads. They are produced under strict quality control standards to ensure dimensional consistency and compatibility with automated magnetic particle processors.
These consumables are widely used in molecular diagnostics, genomics, biobanking, pharmaceutical research, and clinical laboratories. Their standardized design supports reproducible extraction performance while maintaining compatibility with automated sample preparation workflows.
For laboratories requiring more general automation-compatible sample processing plates, deep well plates provide SBS-format compatibility with liquid handling systems from Hamilton, Tecan, Beckman Coulter, and other automation platforms. Immunoassay and ELISA workflows, however, require specialized assay plates designed for optical detection rather than magnetic bead processing.&nbsp;
Lock your combs, load your plates, and let the automation handle the precision. Request a quote from <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">the team at Molecular Biology Products Inc</a> today.

Kingfisher Flex Consumables

KingFisher Flex Consumables for 96 Deep-Well Format

MBPDSW22 by MBP: 96 deep-well V-bottom polypropylene consumables for the Thermo Scientific KingFisher Flex, Duo Prime, Presto, and MagMAX 96. Stocked in US and Canadian warehouses; six-year zero-quality-issue record. Contact MBP for volume pricing.

2.2ml Deep Well Plates for Kingfisher 50/Case

96 Tip Combs for Kingfisher 100/Case

200ul Elution Plate for Kingfisher 50/Case

<h2 dir="ltr">What Is a Hot Start Taq Master Mix?</h2>
 Hot start 2x master mixes combine the convenience of the ready-to-use master mix format with the improved specificity of hot-start technology. These products are the standard reagent format for high-throughput genotyping, clinical diagnostic PCR, and multiplex reactions where non-specific amplification would compromise results. The right hot-start master mix supports cleaner amplification and streamlined setup. Select a formulation designed for reliable PCR performance.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Hot-start formulations from Azura, Accuris, BlasTaq, and Zymo Research for reduced non-specific amplification and primer-dimer formation.</li>
<li>Antibody-mediated hot-start activation in selected products, including ZymoTaq PreMix and Accuris Hot Start Taq Master Mix.</li>
<li>2X master mix and premix formats containing hot-start Taq polymerase, MgCl₂, dNTPs, and optimized PCR buffers.</li>
<li>Red-dye formulations for direct gel loading, including Azura 2X HS Taq Red Mix and Accuris Hot Start Taq Master Mix Red Dye.</li>
<li>Buffer systems optimized for endpoint PCR, multiplex PCR, and challenging template amplification.</li>
</ul>
&nbsp;
<h2 dir="ltr">How to Choose a Hot Start Taq Master Mix</h2>
 Activation Mechanism Format Antibody-mediated hot-start master mixes activate during the standard initial denaturation step; chemically modified formats may require a longer or dedicated activation incubation &mdash; confirm cycling protocol compatibility before adopting a new product. Component Pre-Optimisation A 2x hot-start Taq master mix is a pre-formulated, double-concentrated solution containing hot-start Taq DNA polymerase, dNTPs, MgCl2, and PCR buffer &mdash; the hot-start polymerase remains inactive at room temperature until the activation step is reached during cycling. Multiplex Compatibility For multiplex PCR (multiple primer pairs in a single reaction), hot-start master mixes substantially reduce primer-primer cross-reactivity compared to standard Taq master mixes, improving reliable detection of all intended targets. Diagnostic and Clinical Applications High-specificity master mixes are the standard reagent format for clinical diagnostic PCR, where false-positive bands from non-specific amplification could affect result interpretation. Throughput and Format 2x hot-start master mixes are designed for high-throughput genotyping pipelines, requiring only template and primer addition &mdash; minimising pipetting steps across large sample sets. 
<h2 dir="ltr"> Specifications Context</h2>
 Hot-start 2x master mixes are set up like standard master mixes&mdash;combined with template and primers at the manufacturer-specified ratio to achieve a 1x working concentration&mdash;but the polymerase remains inactive until the activation step, typically during initial denaturation for antibody-mediated formats. This combination of convenience and specificity has made hot-start master mixes a widely used choice for high-throughput genotyping, multiplex PCR, and other applications requiring reliable target-specific amplification. Product availability reflects MBP's catalogue as of mid-2026. Labs validating a new hot-start master mix should evaluate performance using known positive and negative samples and document results by lot number to support quality assurance, audits, and troubleshooting. 
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and get premium-quality Hot Start Taq Master Mix formulations for your lab today.

Hot Start Taq Master Mix

DataSheet

PCR Product Guide

Kodaq 2X PCR MasterMix

G883 Kodaq 2X PCR MasterMix by Applied Biological Materials is a ready-to-use 2X master mix containing Kodaq DNA Polymerase with 3&prime;&rarr;5&prime; proofreading activity, dNTPs, optimized buffer, and gel loading dye. Delivers 800 reactions (10.0 ml); tolerates A/T-rich, G/C-rich, and inhibitor-containing templates.

Note

Kupffer Cell Maintenance Medium

Kupffer Cell Maintenance Medium (TM085) by Applied Biological Materials is a 250 ml proprietary sterile ready-to-use medium for long-term in vitro maintenance of Kupffer cells after the initial plating phase. Use after TM084 (Kupffer Cell Plating Medium). Lot-tested for sterility, pH, osmolality, and endotoxin. Store at 2–8°C.

Caution

Kupffer Cell Plating Medium

Kupffer Cell Plating Medium (TM084) by Applied Biological Materials is a 250 ml proprietary sterile liquid medium formulated for the initial plating and attachment of Kupffer cells (hepatic macrophages) in vitro. Lot-tested for sterility, pH, osmolality, and endotoxin. Store at 2–8°C. Research use only.

<h2>What are bisulfite conversion reagents and buffers?</h2>
&nbsp;
Bisulfite conversion reagents include sodium bisulfite solution, denaturation and conversion buffers, and complete kits that bundle all components needed for the three-step chemical reaction: sulfonation of cytosine to a cytosine-bisulfite derivative, hydrolytic deamination to a uracil-bisulfite derivative, and alkali desulfonation to yield uracil. This process converts unmethylated cytosines to uracil while leaving 5-methylcytosine intact and unconverted, because 5-methylcytosine resists bisulfite-mediated deamination under standard conditions.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">RNA and DNA conversion reagents for enhanced modification of cytosine in methylation workflows</li>
<li dir="ltr" role="presentation">Desulphonation buffers for stabilization of nucleic acids and post-conversion cleanup&nbsp;</li>
<li dir="ltr" role="presentation">Binding buffers used during purification for efficient capture of converted RNA and DNA</li>
<li dir="ltr" role="presentation">Wash buffers for the removal of inhibitors and improved purity of samples</li>
<li dir="ltr" role="presentation">Elution buffers for high-quality recovery of converted nucleic acids</li>
<li dir="ltr" role="presentation">DNA denaturing and digestion buffers for controlled workflow and optimized conversion efficiency</li>
<li dir="ltr" role="presentation">Dilution and reaction buffers for maintaining stable assay conditions</li>
<li dir="ltr" role="presentation">Solubilization and dissolution buffers for sample processing and preparation&nbsp;</li>
<li dir="ltr" role="presentation">Lightning conversion reagents for efficient and rapid methylation analysis workflows</li>
</ul>
&nbsp;
<h2>How to use bisulfite conversion reagents</h2>
&nbsp;
Ensure complete DNA denaturation before conversion to avoid incomplete conversion
Bisulfite only acts on cytosines in single-stranded DNA, so complete denaturation of the input DNA is the most critical first step, since failure to fully denature can leave double-stranded regions in which cytosines are shielded from the bisulfite reagent and remain unconverted.
Understand that incomplete conversion produces false-positive methylation results
If the conversion is incomplete, unconverted unmethylated cytosines will read as cytosine after sequencing, which will be incorrectly interpreted as methylated cytosine, artificially inflating the apparent methylation level of the sample in a false-positive direction.
Optimize conversion conditions to balance completeness against DNA degradation
The harsh conditions needed for complete bisulfite conversion, including high bisulfite concentration, elevated temperature, and long incubation, also fragment and degrade DNA, and optimizing conditions that achieve high conversion while minimizing degradation is a key technical challenge in bisulfite workflows.
Include an unmethylated conversion control to estimate residual incomplete conversion
Including an unmethylated control DNA, such as lambda phage DNA, in the same conversion reaction provides a readout of how completely unmethylated cytosines were converted, helping quantify residual incomplete conversion rates and account for them in downstream analysis.
Be aware of specific challenges for FFPE and degraded DNA samples
Formalin-fixed, paraffin-embedded tissue DNA is already fragmented before bisulfite treatment, and the additional fragmentation during conversion can result in poor library yields; specific modified protocols and carrier reagents can help reduce further degradation during the conversion step.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis due to its high resolution and accuracy at single-base level, but it also introduces specific DNA quality challenges for downstream library preparation and sequencing, particularly for samples already of poor quality like FFPE tissue. As of 2026, enzymatic conversion is increasingly recommended as the preferred alternative to bisulfite conversion specifically because it achieves comparable discrimination between methylated and unmethylated cytosines while minimizing the DNA damage and library complexity loss that harsh bisulfite chemistry introduces.
This category sits within DNA/RNA Methylation under Epigenetics, and for a synthetic, fully methylated positive control to run through the conversion workflow, see dNTPs (Deoxynucleotide Triphosphates). See the full reagents catalog and use Quick Order for recurring orders.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP and request a quote today to get high-quality bisulfite conversion reagents for your lab.

Bisulfite Conversion Reagents & Buffers

L-Desulphonation Buffer

D5046-5 by Zymo Research — L-Desulphonation Buffer; 80 mL; for in-column desulphonation of bisulfite-converted DNA; compatible with EZ DNA Methylation-Lightning kits (D5046, D5047); made in USA.

L-Desulphonation Buffer (80 ml)

L-Desulphonation Buffer (40 ml)

L-Desulphonation Buffer (10 ml)

<h2>What are L-glutamine and amino acid supplements?</h2>
&nbsp;
L-glutamine and amino acid supplements are cell culture additives used to support cellular metabolism, growth, viability, and biosynthesis. They provide nutrients that may become depleted during culture or are required in greater quantities than those supplied by basal media alone. These supplements are commonly added to media such as DMEM, RPMI 1640, MEM, and DMEM/F-12 to optimize cell growth and maintain healthy cultures.
L-glutamine is one of the most important supplements in mammalian cell culture because it serves as a major energy source and is required for protein synthesis, nucleotide production, and glutathione synthesis. However, because free L-glutamine is unstable in solution and gradually breaks down into ammonia and pyroglutamate, many laboratories use stabilized alternatives such as GlutaMAX (L-alanyl-L-glutamine) for extended culture applications.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>L-Glutamine stable formulations for sourcing of essential nitrogen</li>
<li>Non-Essential Amino Acids (NEAA) for growth media enhancement</li>
<li>Synthetic Polymer Coatings Poly-L-Ornithine and Poly-L-Lysine solutions for better cell attachment.</li>
<li>Extracellular Matrix Proteins used for cell adhesion and include high-purity Fibronectin and Vitronectin coating reagents</li>
<li>Adhesion Promotion Reagents for improving the surface properties of culture vessels.</li>
</ul>
&nbsp;
<h2>How to choose L-glutamine and amino acid supplements</h2>
&nbsp;
L-glutamine concentration and format
Most mammalian cell culture systems use L-glutamine concentrations between 2&ndash;6 mM, although requirements vary depending on the medium and cell type. It is commonly supplied as:
<ul>
<li>USP-grade powder for custom media preparation</li>
<li>200 mM liquid stock solutions for direct supplementation</li>
</ul>
Liquid stocks should be aliquoted and stored frozen to minimize repeated freeze-thaw cycles and preserve stability.
Understanding glutamine degradation
Free L-glutamine gradually breaks down in aqueous solution through a non-enzymatic process that produces ammonia and pyroglutamate. This degradation accelerates with increasing temperature, storage time, and exposure to common cell culture buffer systems.
Ammonia accumulation can negatively affect cell growth, viability, productivity, and experimental reproducibility, particularly during long culture periods without media replacement.
GlutaMAX and stable glutamine alternatives
GlutaMAX (L-alanyl-L-glutamine) is a stabilized dipeptide form of glutamine designed to resist degradation in culture media. Once taken up by cells, it is converted into usable glutamine while maintaining greater stability during incubation.
<ul>
<li>Reduced ammonia accumulation</li>
<li>Improved media stability</li>
<li>Better performance in long-term cultures</li>
<li>Less frequent media replacement requirements</li>
</ul>
For most applications, GlutaMAX can directly replace standard L-glutamine without protocol modifications.
MEM non-essential amino acids (NEAA)
NEAA supplements provide amino acids that cells can synthesize themselves but may benefit from receiving directly from the culture medium. Supplementation reduces the metabolic burden of amino acid production and often improves growth rates and cell viability.
NEAA solutions are typically supplied as 100X concentrates and diluted to 1X working concentration.
HT supplement
HT supplement contains hypoxanthine and thymidine and is commonly used following HAT selection during hybridoma production. It supports nucleotide synthesis through salvage pathways while cells recover from selection pressure.
MEM vitamin solution
Vitamin supplements provide additional growth-supporting micronutrients that may become limiting during prolonged culture or in demanding applications. These supplements are often used alongside amino acid supplementation to enhance culture performance.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The standard L-glutamine concentration used in many mammalian culture systems is approximately 2 mM, though actual requirements vary by medium formulation and cell type. Free L-glutamine remains stable as a dry powder and frozen stock solution but degrades progressively once added to liquid media.
Stabilized glutamine products such as GlutaMAX have become increasingly common because they maintain glutamine availability while minimizing ammonia accumulation during incubation. These formulations are particularly valuable in long-duration experiments and high-density culture systems.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to get research-grade supplements for your lab.

L-Glutamine & Amino Acids

L-Glutamine Solution (200 mM)

G275 by Applied Biological Materials (ABM) is a 200 mM L-Glutamine solution (29.2 g/L) in normal saline, 0.2 µm sterile-filtered. Supplied in 100 mL. Passes sterility testing. Store at −20°C; use within 2 weeks of thawing. For use as a cell culture media supplement.

Usage

Dry Baths

<h2>What Are Dry Bath Incubators?</h2>
&nbsp;
A dry bath incubator uses direct thermal conduction from a heated aluminum block to sample tubes placed in precision-machined well inserts, rather than immersing samples in a liquid medium. This eliminates water bath contamination risks from microbial biofilms, cross-contamination between samples from water droplets, and the mineral scale buildup associated with tap water. Aluminum blocks heat rapidly: most models reach 37 degrees C from ambient in 3-5 minutes and 100 degrees C in approximately 10-15 minutes, providing faster protocol transitions than water baths for multi-temperature workflows. Interchangeable aluminum well insert blocks are machined to fit specific tube formats with tight tolerances (typically plus or minus 0.1 mm), maximizing thermal contact between the block wall and tube exterior for efficient and uniform heat transfer. Choose dry bath incubators over water baths when contamination prevention is critical, when multiple setpoint temperatures are needed in rapid succession, or when protocols require temperatures above 70 degrees C, where evaporation from water baths is significant.
&nbsp;
<h2 dir="ltr">What You Will Find:</h2>
&nbsp;
<a href="https://mbpinc.net/equipments/temperature-control-equipment/temperature-controlled-baths/dry-baths/dry-baths-accessories" title="Dry Baths Accessories">Dry Baths Accessories</a>
<ul>
<li dir="ltr" role="presentation">High-conductivity interchangeable heating blocks designed to accommodate microtubes, PCR strips, and centrifuge tubes for maximum versatility.</li>
<li dir="ltr" role="presentation">Specialized block lifters and thermal covers that ensure safe handling and improved temperature insulation during high-heat protocols.</li>
<li dir="ltr" role="presentation">Precision-fit modular inserts engineered for seamless heat transfer, allowing you to customize your heating station for any vessel size.</li>
</ul>
&nbsp;
<h2>How to Choose a Dry Bath Incubator</h2>
&nbsp;
Number of blocks and block capacity
Single-block dry baths hold one aluminum insert block and typically accept 8-24 wells, depending on tube size, occupying minimal bench space. Dual-block models accept two independently controlled blocks simultaneously (see Dual Baths), enabling two temperatures in one footprint. Four-block configurations (such as Labnet DB-04A) hold four blocks with a broad temperature range up to 150 degrees C, suitable for high-throughput labs processing multiple protocols simultaneously. Confirm well count per block for your primary tube format before purchasing.
Temperature range
Standard dry bath incubators cover ambient plus 5 degrees C to 100 degrees C, sufficient for most molecular biology protocols (37 degrees C incubation, 55-60 degrees C Proteinase K digestion, 70 degrees C inactivation, 95 degrees C DNA denaturation). Extended-range models reach 120-160 degrees C for applications such as paraffin embedding at 60-65 degrees C, wax melting, electrophoresis gel denaturation, and COD (chemical oxygen demand) digestion at 150 degrees C. Models with Peltier cooling blocks extend the lower range to -10 to -30 degrees C for sub-ambient incubations.
Temperature accuracy and uniformity
Block temperature accuracy (deviation from the true temperature at setpoint) is typically plus or minus 0.2-0.5 degrees C at 37 degrees C for microprocessor PID-controlled units; the Biolab dry bath incubator 1306 series specifies plus or minus 0.3 degrees C stability and or minus 0.3 degrees C block uniformity at 100 degrees C. Block uniformity describes the temperature variation between different wells in the same insert at the same setpoint, a critical specification when running comparative assays where all samples must be at identical temperatures.
Heated lid
An adjustable heated lid positions a temperature-controlled surface directly above the tube caps during incubation, preventing condensation of volatile samples on the cooler upper portions of the tube and the cap. Heated lids are essential for enzyme reactions, isothermal amplification (LAMP), immunoassay incubations, and any protocol where evaporation or condensation affects sample concentration or reaction outcome. Models like the Labnet PR1MA Mini with Heated Lid allow independent temperature setting of the lid to optimize anti-condensation heating without overheating the sample.
Programmable timer and multi-cycle function
Programmable dry baths with a built-in timer (up to 99 hours and 59 minutes on most models) allow unattended protocol completion with automatic shutoff. Multi-cycle function (up to 99 repetitions) enables automated ramp-hold-repeat cycles for denaturation-renaturation experiments, isothermal nucleic acid amplification protocols, and temperature cycling without a PCR thermocycler for simple two-step protocols. Auto-restart after power failure is standard on research-grade models.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
A 150 W heating element in a single-block dry bath achieves temperature equilibration from 25 degrees C to 100 degrees C in 10 minutes or less, compared to 15-25 minutes for a 12-20 L water bath at comparable setpoints. Block temperature uniformity of plus or minus 0.3 degrees C across all wells is standard for research-grade dry bath incubators; economy models may specify plus or minus 1 degree C block uniformity, which is insufficient for quantitative assays comparing samples run in different wells. Dry bath incubators operating at 95-100 degrees C require minimal maintenance compared to water baths: no water changes, no algae treatment, no mineral descaling, making them operationally lower-cost for high-temperature molecular biology protocols. Dry bath incubators with heated lids, dual LED displays (showing actual and setpoint temperatures simultaneously), and programmable multi-cycle timers are the standard specification for new molecular biology lab installations, replacing older dry block heaters.&nbsp;
&nbsp;
Ready to upgrade your sample heating setup? Explore our full range of dry baths and <a href="https://mbpinc.net/contact-us">contact the MBP team</a> for a quote today.

Product Literature

D1300-Group-Brochure

D1300-Group-Constant Temperature Selection Guide

Block, 48 x 0.2 ml PCR tubes or 6 x 0.2 ml

Single Block, 96 well PCR plate, skirted or nonskirted (for single block unit only)

Dual Block, 96 well micro plate or 4 slides (for dual block unit only)

Dual Block, 96 well PCR plate, skirted or nonskirted (for dual block unit only)

Block, 384 well PCR plate. Block fits D1200 only and holds one plate

Pop-Stopper for D1102, D1102-A, D1105, D1105-A blocks

AccuRack™ tube rack for 20x1.5mL tubes. Compatible with the D1105A block

AccuBlock digital dry bath Accessories

LABNET DIGITAL DRY BATH Series

D1300 series by Labnet International is the AccuBlock™ Digital Dry Bath — ambient +5°C to 150°C, ±0.2°C uniformity, built-in 99-hour timer. Available in 1-block (D1301), 2-block (D1302), and 4-block (D1304) configurations with 120 V and 230 V variants. Accepts the full Labnet interchangeable block system.