Brands

<h2>What are microbial and environmental DNA isolation kits?</h2>
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Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
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<h2>How to choose a microbial and environmental DNA isolation kit</h2>
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Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
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<h2>Specifications context</h2>
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The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
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MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

D4310-Data Sheet

D4310-MSDS

D4310-1-20-MSDS

D4310-2-5-MSDS

D4310-3-50-MSDS

D4310-Protocol

D4310-Optimized Lysis Protocols

HostZERO™ Microbial DNA Kit (50 Preps.)

D4310 by Zymo Research — HostZERO™ Microbial DNA Kit (50 preps) for host DNA depletion (≥ 90%) and microbial DNA isolation (≥ 85% recovery) from saliva, swabs, and bodily fluids. Reduces human DNA in saliva from ~65% to &lt; 1%. Under 90 min total; 20 µl elution. All 15 components included for complete workflow.

DNA Purity

DNA Recovery

Processing Time

Required Equipment

Sample Input

Sample Type

Manufacturer

Shipping Condition

Storage Condition

Host Depletion Solution (20 ml)

Microbial Selection Buffer (5 ml)

Microbial Selection Enzyme (50 µl)

<h2>What Are Laboratory Recirculating Chillers?</h2>
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Laboratory recirculating chillers are compressor-based cooling instruments that circulate chilled fluid through a closed loop to remove heat from connected equipment or maintain below-ambient temperatures in a reactor, condenser, or instrument cooling line. Unlike tap-water cooling, recirculating chillers reuse coolant, eliminating water waste and ensuring stable setpoint temperatures independent of building water supply variations. Applications include rotary evaporator condenser cooling, jacketed reactor temperature control, protection of vacuum pump oil from vapor contamination, cooling of laser cavities, NMR superconducting magnets, and mass spectrometers. Modern air-cooled chillers achieve a coefficient of performance (COP) of approximately 3:1, meaning 1 kW of electrical input removes 3 kW of heat load. Choose a benchtop recirculating chiller for small-scale solvent recovery and instrument cooling; choose a floor-standing high-capacity model for jacketed reactors and multi-instrument cooling networks.
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<h2 dir="ltr">What You Will Find:</h2>
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Ai Recirculating Chillers offer a user-friendly and compact solution for everyday cooling, featuring powerful centrifugal pumps that keep your routine benchtop tasks moving smoothly.
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Julabo Dynamic Temperature Control units bring incredible speed to your workflow, allowing you to jump between extreme heat and cold with the precision your specialized research demands.
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HUBER Unistat and OLED Chillers make monitoring a breeze with crystal-clear displays and smart interfaces, putting total control of your chemical reactors right at your fingertips.
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PolyScience Immersion Coolers give you the flexibility to drop temperatures quickly in any bath or tank using high-tech probes and large, easy-to-read digital displays.
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Huber Air-Cooled Immersion Units handle the toughest ultra-low cooling jobs with ease, providing heavy-duty performance without the hassle of extra plumbing or water lines.
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</ul>
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<h2>How to Choose a Lab Recirculating Chiller</h2>
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Cooling capacity at target temperature
Manufacturers publish cooling capacity in watts or kW at specific setpoint temperatures. A chiller with 500 W capacity at -20 degrees C may deliver only 200 W at -40 degrees C. Match cooling capacity to the actual heat load of the connected equipment at the required operating temperature, not the ambient-temperature rating.
Temperature range
Benchtop recirculating chillers reach -10 degrees C to -30 degrees C for most solvent recovery and condenser cooling applications. Deep-cooling models reach -40 degrees C to -80 degrees C for cryogenic trapping, lyophilizer condenser cooling, and temperature-sensitive biological workflows. Mechanical cold traps used with vacuum lines typically require -40 degrees C or colder.
Air-cooled vs. water-cooled
Air-cooled chillers dissipate heat via a fan into the room air and require no plumbing. They are the standard for bench-scale labs, but raise the room temperature and reduce efficiency when the ambient temperature exceeds 25 degrees C. Water-cooled chillers are more efficient at high heat loads and in warm environments, but require facility supply and drain connections.
Reservoir volume and flow rate
Reservoir volume (4 L to 60 L) determines thermal buffering capacity; larger volumes smooth out temperature fluctuations during high-load cycles. Flow rates of 10 L/min to 42 L/min are typical. External applications with long tubing runs or large jacketed vessels require higher flow rates to minimize temperature rise along the circuit.
Coolant compatibility
Most lab chillers use deionized water or water-ethylene glycol mixtures as coolant. For applications requiring temperatures below -20 degrees C, a 30-50% ethylene glycol mixture is standard to prevent freezing in the circuit. Confirm the chiller's internal materials (stainless steel, polypropylene, or PTFE wetted parts) are compatible with the intended coolant.
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<h2>Specifications Context</h2>
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Confirm coolant type compatibility, pump flow rate at target temperature (not ambient), and electrical requirements: 120V/60Hz for North America or 208-240V for high-capacity models. Modern environmentally responsible chillers use natural hydrocarbon (HC) refrigerants with low global warming potential (GWP). Closed-loop systems with optical fluid level sensors reduce maintenance downtime compared to mechanical float switches. Chillers maintaining temperature within plus or minus 0.1 degrees C or better are appropriate for precision analytical instrument cooling.
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Ready to beat the heat in your lab? Take a look through our chiller collection and <a href="https://mbpinc.net/contact-us">contact the MBP team</a> for a friendly quote today.

Chillers

HUBER TC100 Air-Cooled -100°C Immersion Cooler

HUBER TC100 Air-Cooled -100°C Immersion Cooler Standard Package

Huber TC100E Spez Air-Cooled-100°C Immersion Cooler

Huber TC100E Spez air-cooled immersion cooler reaches -100°C with ±0.5 K stability, two-stage R-452A/R-1150 refrigerant circuit, 220–240 V/6.5 A, and 295×500×570 mm footprint. No internal pump — immerses directly into external vessel or bath.

<h2 data-section-id="1yjaxog" data-start="0" data-end="23">What Are IHC Slides?</h2>
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IHC slides, also known as charged slides or adhesion slides, are microscope slides designed specifically for immunohistochemistry (IHC), histology, cytology, and pathology applications. These slides feature a permanently charged surface that helps tissue sections and cellular samples remain attached during staining procedures, heat-induced epitope retrieval (HIER), antibody incubations, and repeated wash steps.
Globe Scientific Diamond&reg; White Glass charged slides are manufactured from optical-quality white glass and come pre-cleaned and ready to use. They are available with either 90&deg; ground edges or 45&deg; beveled safety corners and are offered in multiple frosted-end color options to simplify specimen identification and laboratory workflow management.
Charged IHC slides are essential for FFPE tissue sections, frozen sections, and cytospin preparations because they help prevent tissue loss during staining and antigen retrieval procedures.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li dir="ltr" aria-level="1">
Human breast cancer control slides
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<li dir="ltr" aria-level="1">
FFPE kidney cancer tissue sections
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<li dir="ltr" aria-level="1">
Human brain tissue microarray slides
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<li dir="ltr" aria-level="1">
Paraffin-embedded colon cancer sections
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<li dir="ltr" aria-level="1">
Intestine cancer diagnostic slides
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<li dir="ltr" aria-level="1">
Positive charged IHC control slides
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<li dir="ltr" aria-level="1">
Formalin-fixed stomach cancer samples
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<li dir="ltr" aria-level="1">
Microtome-sectioned pathology slides
</li>
</ul>
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<h2 data-section-id="1p4j59x" data-start="990" data-end="1017">How to Choose IHC Slides</h2>
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Hydrophobic vs Hydrophilic Surface
Most charged IHC slides use a hydrophobic positively charged surface, making them suitable for routine FFPE tissue staining, frozen sections, and cytology applications. These slides provide reliable tissue adhesion during manual immunohistochemistry workflows.
Hydrophilic charged slides are commonly used with automated staining systems where uniform reagent distribution across the slide surface is important. These specialty slides are often selected for automated IHC platforms and high-throughput laboratory workflows.
Adhesion Strength
Standard charged slides provide strong tissue adhesion suitable for most routine immunohistochemistry applications, including common antigen retrieval protocols and standard staining procedures.
For challenging specimens such as fragile frozen sections, decalcified tissues, or protocols involving extended high-temperature antigen retrieval, laboratories may choose specialty slides designed to provide enhanced adhesion performance.
Edge Style
Slides with 90&deg; ground edges provide a smooth finish that works well with automated staining and coverslipping systems.
Slides with 45&deg; beveled safety corners help reduce the risk of cuts during handling and are often preferred in laboratories that process large numbers of slides daily.
Frosted Ends and Color Coding
Frosted labeling areas allow specimen information to be written directly on the slide. Color-coded frosted ends can help distinguish patient samples, staining batches, and laboratory workflows. Multiple color options are available to support organization in busy pathology and histology laboratories.
Packaging Options
IHC slides are available in various box and case quantities to accommodate laboratories of different sizes. High-volume pathology laboratories often purchase slides in case quantities to ensure a consistent supply for routine diagnostic and research work.
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<h2 data-section-id="1jl751w" data-start="3652" data-end="3677">Specifications Context</h2>
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Globe Scientific Diamond White Glass charged microscope slides are manufactured from optical-quality white glass and feature a permanent positive surface charge. Standard dimensions are approximately 25 &times; 75 mm with a thickness of about 1 mm. Slides are supplied pre-cleaned and ready for laboratory use.
Available options include 90&deg; ground edges or 45&deg; beveled safety corners, along with frosted-end labeling areas in white, blue, red, green, yellow, orange, aqua, pink, and lavender.
Packaging formats include boxes containing 72 or 144 slides, with larger case quantities available for high-volume laboratories. Globe Scientific products are manufactured under ISO 9001:2015 quality standards.
Ready to streamline your pathology validation? Request a quote from the <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">helpful team at MBP today</a>.

IHC Slides

Human Bladder

Human Bladder FFPE Tissue Section HS0040 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Unit Quantity

Guarantee

Shipping condition

Human Brain

Human Brain FFPE Tissue Section HS0021 — 4 µm formalin-fixed paraffin-embedded sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and neuropathology research. Research Use Only.

Concentration

Human Breast

Human Breast FFPE Tissue Section HS0048 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Human Breast Cancer HS0001

HS0001 Human Breast Cancer FFPE Tissue Sections by ABM — 4 µm sections on positively charged glass slides; H&E QC-stained; 6 slides/pack. Store at cool dry RT or 4°C; 1-year expiry. For IHC, ISH, and breast cancer biomarker research.

Human Breast Cancer HS0002

HS0002 Human Breast Cancer FFPE Tissue Sections by ABM — 4 µm sections on positively charged glass slides; H&E QC-stained; 6 slides/pack. Store at cool dry RT or 4°C; 1-year expiry. For IHC, ISH, and breast cancer biomarker research.

Human Breast Cancer HS0003

Human Breast Cancer FFPE Tissue Section HS0003 — 4 µm formalin-fixed paraffin-embedded sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Human Breast Cancer HS0004

Human Breast Cancer FFPE Tissue Section HS0004 — 4 µm formalin-fixed paraffin-embedded sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Human Breast Cancer HS0005

Human Breast Cancer FFPE Tissue Section HS0005 — 4 µm formalin-fixed paraffin-embedded sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Human Breast Ductal Carcinoma

Human Breast Ductal Carcinoma FFPE Tissue Section HS0041 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.

Human Breast Invasive Carcinoma

Human Breast Invasive Carcinoma FFPE Tissue Section HS0042 — 4 µm FFPE sections on positively charged glass slides, H&amp;E stained QC. 6 slides/pack. For IHC, ISH, and histopathology. Research Use Only.