Brands

Transfer Pipettes

<h2>What are disposable transfer pipettes?</h2>
&nbsp;
Disposable transfer pipettes are single-use plastic liquid-handling tools made from low-density polyethylene (LDPE). They are designed as a one-piece bulb-and-stem system used for transferring, dispensing, and handling small volumes of liquids in laboratory workflows.
They are commonly used as a safer and more convenient alternative to glass Pasteur pipettes. Being plastic and disposable, they eliminate the risk of breakage, reduce cross-contamination, and remove the need for separate rubber bulbs. The flexible bulb allows controlled aspiration and dispensing of reagents, samples, and solutions.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Graduated transfer pipettes &ndash; for approximate volume measurement and controlled dispensing</li>
<li>Non-graduated transfer pipettes &ndash; for simple liquid transfer without measurement markings</li>
<li>Fine-tip transfer pipettes &ndash; for small droplets and precise spot dispensing</li>
<li>Sterile transfer pipettes &ndash; for microbiology and contamination-sensitive workflows</li>
<li>Non-sterile transfer pipettes &ndash; for routine laboratory use</li>
<li>Disposable Pasteur pipettes &ndash; for general single-use liquid handling</li>
</ul>
&nbsp;
<h2>How to choose transfer pipettes</h2>
&nbsp;
Tip style Fine-tip pipettes are used for precise work such as microplate spotting and small-volume handling. Standard tips are suitable for general transfers, while wide-bore designs are better for viscous liquids or blood samples. Longer stems help access deep containers.
Capacity and volume handling Select pipettes based on typical working volumes. Smaller pipettes are suitable for low-volume transfers, while larger ones are used for routine sample handling. The effective draw volume depends on bulb capacity and usage technique.
Graduated vs non-graduated Graduated pipettes allow approximate volume estimation and are useful in clinical and diagnostic workflows. Non-graduated pipettes are intended for general dispensing where precise measurement is not required.
Sterility requirements Sterile pipettes are used in microbiology, cell culture, and clinical applications where contamination control is essential. Non-sterile versions are suitable for general laboratory and teaching use.
Length and accessibility Long pipettes are preferred for deep vessels such as flasks and bottles, while shorter designs are used for compact tubes and small workspaces.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Disposable LDPE transfer pipettes are widely used laboratory consumables for general-purpose liquid handling in research, clinical, and educational environments. They are available in sterile and non-sterile formats with multiple tip designs and capacities to support different workflows.
These pipettes remain a standard alternative to glass Pasteur pipettes due to their safety, low cost, and reduced contamination risk in routine laboratory applications.
For bulk orders or lab-specific requirements, <a href="https://mbpinc.net/contact-us">contact the MBP team</a> for assistance and pricing.

139110-Group-Transfer Pipet - Brochure

Exact Volume Transfer Pipettes Specifications

Exact Volume Transfer Pipettes

Globe Scientific 139110 — 50 µL exact volume LDPE transfer pipette, 59 mm long. Dispenses a fixed 50 µL at full bulb compression. Pack of 5,000 (500/bag × 10 bags). Suits fixed-volume reagent addition, blood transfer, and point-of-care spot testing. Related sizes: 100 µL (139114), 200 µL (139116), 250 µL (139118).

Manufacture

Transfer Pipet, Exact Volume, 50uL (0.05mL), 59mm Long, 500/Pack, 10 Packs/Case

Transfer Pipet, Exact Volume, 75uL (0.075mL), 75mm Long, 500/Pack, 10 Packs/Case

Transfer Pipet, Exact Volume, 100uL (0.10mL), 83mm Long, 500/Pack, 10 Packs/Case

Transfer Pipet, Exact Volume, 200uL (0.20mL), 115mm Long, 500/Pack, 10 Packs/Case

Transfer Pipet, Exact Volume, 250uL (0.25mL), 102mm Long, 500/Pack, 10 Packs/Case

Vacuum Pump

<h2>What are laboratory vacuum pumps?</h2>
&nbsp;
Laboratory vacuum pumps are instruments that remove gas from a connected vessel or system to create sub-atmospheric pressure for applications including rotary evaporation of solvents, vacuum filtration through B&uuml;chner funnels and membranes, SpeedVac centrifugal concentration, gel drying, lyophilization rough vacuum, and degassing of solvents and solutions. MBP carries vacuum pumps from Vacuubrand, Welch, and ULVAC, which together cover the full range of laboratory vacuum needs from moderate filtration vacuum to deep vacuum for turbomolecular backing, available in oil-free diaphragm and oil-sealed rotary vane configurations.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul style="list-style-type: disc;">
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/edwards-pumps" style="color: #236fa1;">Edwards Pumps: </a>Reliability at its best &ndash; with strong vacuum pumps that are made to work for high-performing industrial and research purposes.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/agilent-pumps" style="color: #236fa1;">Agilent Pumps: </a>Get the most out of high vacuum pumps engineered for mass spectrometry, leak testing, and other advanced lab methods.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/vacuubrand-pumps">Vacuubrand Pumps: </a>Take advantage of German-engineered diaphragm pumps and vacuum control devices that are resistant to chemical agents and easy to maintain.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/welch-pumps">Welch Pumps: </a>Perfect for all your routine lab procedures using vacuum pumps made with quality materials for durability and versatility.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/ulvac-pumps" style="color: #236fa1;">ULVAC Pumps:</a> Use proven Japanese vacuum technology renowned for reliable and effective operations in manufacturing and thin-film production.</li>
<li dir="ltr"><a href="https://mbpinc.net/equipments/sample-processing/pumps/vaccum-pumps/vaccum-pump-accessories" style="color: #236fa1;">Vacuum Pump Accessories:</a> Boost your system&rsquo;s capabilities with oil mist filters, traps, and other accessories to optimize pump performance.</li>
</ul>
&nbsp;
<h2>How to choose between vacuum pump brands and types</h2>
&nbsp;
Choose Vacuubrand for premium PTFE diaphragm and chemistry vacuum systems
Vacuubrand diaphragm pumps are known for their high-quality PTFE-wetted construction, precise chemistry vacuum control systems with automatic set-point regulation, and a broad range of configurations from single-stage to two-stage models, making them the preferred brand for demanding rotary evaporation and concentration applications where reproducible vacuum control is important.
Choose Welch for reliable, cost-effective diaphragm and corrosion-resistant vacuum
Welch (by Gardner Denver) diaphragm pumps offer solid performance for routine filtration and moderate vacuum applications at a competitive price point, with the Dryfast ULTRA series as their flagship oil-free, chemically resistant product line covering most common laboratory vacuum needs.
Choose ULVAC for applications requiring deeper vacuum
ULVAC rotary vane oil-sealed pumps achieve deeper ultimate vacuum levels than oil-free diaphragm pumps, making them appropriate for applications like thin-film deposition, high-vacuum instruments, manifold vacuum systems, or backing turbomolecular pumps, where vacuum levels below 1 mbar are required.
Check the accessories catalog for vacuum tubing, traps, and gauges
Accessories include cold traps and chemical traps to protect pumps from solvent and corrosive vapors, vacuum tubing (rubber, silicone, or PTFE), and vacuum gauges and controllers for monitoring and regulating applied vacuum.
Match ultimate vacuum and pumping speed to your application requirements
Ultimate vacuum determines the lowest pressure achievable, while pumping speed determines how quickly the system reaches and maintains that vacuum. Both must be matched to system volume and gas load from evaporation, filtration, or outgassing.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The choice between oil-free diaphragm and oil-sealed rotary vane pumps is primarily determined by required ultimate vacuum and chemical exposure. Diaphragm pumps provide oil-free, low-maintenance vacuum suitable for most chemistry and biology applications above ~1&ndash;5 mbar, while rotary vane pumps achieve deeper vacuum but require oil changes and carry contamination risk.
As of 2026, oil-free diaphragm pumps from Vacuubrand and Welch cover most standard molecular biology and chemistry vacuum applications, while ULVAC rotary vane and dry pumps serve specialized deep-vacuum requirements.
Do you need any assistance figuring out your displacement or pressure requirements? Our experts at MBP are here to help. <a href="https://mbpinc.net/contact-us">Contact us for a customized quote.</a>

Vaccum Pump

Exhaust Silencer Filter for Edwards nXDS Series Vacuum Pumps

The ES10 by Edwards is an exhaust silencer filter for Edwards nXDS6i, nXDS10i, nXDS15i, and nXDS20i dry scroll vacuum pumps. It combines multi-stage noise reduction with integrated tip-seal dust filtration, mounts vertically or horizontally, and uses a patented high-grade filter element with butterfly gasket for tool-free element changes. Recommended when the pump is not connected to a house exhaust system.

<h2 data-section-id="b4wzzk" data-start="0" data-end="47">What is bacterial culture media for cloning?</h2>
&nbsp;
Bacterial culture media for cloning workflows includes LB (Luria-Bertani) broth and agar, the standard general-purpose media for E. coli growth, SOC media used for post-transformation recovery, and Terrific Broth for higher-density culture in plasmid preparation. Pre-made agar plates with antibiotic selection (ampicillin, kanamycin, chloramphenicol, and others matching common selection markers) are used to isolate colonies carrying the desired plasmid. Related entities include antibiotic selection markers matching transformation kit resistance genes, post-transformation recovery time, and plasmid yield scaling with culture volume and media richness.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">ZymoBroth Growth Media for efficient transformation workflows to generate highly competent bacterial cells</li>
<li dir="ltr" role="presentation">Overexpression Broth (OB) optimized for increasing recombinant protein yields with high-density nutrient media.</li>
<li dir="ltr" role="presentation">Expansion Broth (EB) for the faster and more reliable expansion of microbial cultures.</li>
<li dir="ltr" role="presentation">Dual Media Sets for integrated growth and induction workflows with the help of combined EB and OB systems</li>
</ul>
&nbsp;
<h2 data-section-id="9xi4x5" data-start="919" data-end="971">How to choose bacterial culture media for cloning</h2>
&nbsp;
LB broth/agar vs. Terrific Broth
LB broth and agar are the standard general-purpose media for routine cloning, colony selection, and small-scale plasmid preparation, while Terrific Broth provides higher nutrient density supporting higher bacterial cell density, making it useful when maximizing plasmid yield in larger-scale preparations.
SOC media for post-transformation recovery
SOC media is richer than standard LB and supplemented with glucose and magnesium. It is commonly used for a brief recovery incubation immediately after heat-shock or electroporation transformation, helping transformed cells recover before plating on selective agar. Some transformation protocols specifically recommend SOC rather than LB for this recovery step.
Antibiotic selection matching
Pre-made antibiotic-selective agar plates must match the resistance marker carried by the transformed plasmid, whether ampicillin, kanamycin, chloramphenicol, or another selection marker. Using the wrong antibiotic&mdash;or no antibiotic at all&mdash;will not properly select for plasmid-containing colonies and may allow unwanted bacterial growth.
Liquid vs. agar plate formats and shelf life
Liquid media (broth) is used for post-transformation recovery and culture scale-up, while agar plates are used for colony isolation and selection. Pre-made antibiotic plates have a defined shelf life because antibiotics gradually lose activity over time, making expiration dates particularly important for antibiotics with lower stability.
&nbsp;
<h2 data-section-id="1fljvas" data-start="3523" data-end="3548">Specifications context</h2>
&nbsp;
LB broth and agar are formulated with defined concentrations of tryptone, yeast extract, and sodium chloride, with agar plates commonly supplied in standard Petri dish sizes (for example, 100 mm) and containing antibiotic concentrations matched to common resistance markers, such as 100 &micro;g/mL ampicillin or 50 &micro;g/mL kanamycin. SOC recovery is typically performed as a short incubation, often around one hour at 37&deg;C with shaking, before plating cells on selective agar.&nbsp;
This sub-category is part of MBP's cloning catalog alongside transformation kits and competent cells, the immediately preceding step, and chemicals/reagents for enzyme-based fragment preparation. Labs scaling up plasmid production for lentivirus packaging in immortalization workflows can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm antibiotic concentration and media format requirements for a specific selection marker before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today to request a quote for high-quality culture media.

Culture Media

M3012-100 - MSDS

Expansion Broth™ (EB™)

Expansion Broth (EB) by Zymo Research: bacterial growth medium that represses recombinant protein expression during the cell expansion phase. Use with Overexpression Broth (OB) for consistent, high-yield recombinant protein production. Room temperature storage. 100 ml and 500 ml sizes available.

Product Storage

Manufacturer

Shipping Condition

Storage Condition

Expansion Broth™ (EB™) (100 ml)

Expansion Broth™ (EB™) (500 ml)

<h2 data-section-id="1h8mvlk" data-start="0" data-end="45">What are primary cell culture medium kits?</h2>
&nbsp;
Primary cell culture medium kits are complete, ready-to-prepare culture systems that combine a cell-type-specific basal medium with the supplements required for optimal growth and maintenance of primary cells. Unlike purchasing media, serum, growth factors, and antibiotics separately, these kits provide all components in validated proportions, ensuring that the medium performs as intended for a specific primary cell type.
A typical kit includes a basal medium, a cell-specific growth supplement, an antibiotic component where appropriate, and fetal bovine serum (FBS) or an equivalent supplement when required. The components are designed to be combined directly according to the manufacturer's instructions, reducing preparation errors and improving consistency between experiments.
&nbsp;
<h2 data-path-to-node="5">What you will find:</h2>
&nbsp;
<ul data-path-to-node="6">
<li style="font-family: arial, helvetica, sans-serif;">
Tissue-specific primary cell culture medium kits
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Specialized growth media for endothelial and epithelial cells
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Workflow systems for thawing, plating, and maintenance of hepatocytes&nbsp;
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Differentiation media for progenitor and stem cells&nbsp;
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Serum-free bioprocessing and cryopreservation reagents
</li>
<li style="font-family: arial, helvetica, sans-serif;">
Targeted fibroblast, smooth muscle, and myocyte growth kits
</li>
</ul>
&nbsp;
<h2 data-section-id="1nip7jz" data-start="1066" data-end="1116">How to choose a primary cell culture medium kit</h2>
&nbsp;
Match the kit to the cell type
Primary cells have highly specialized nutritional and signaling requirements. Kits are available for endothelial cells, hepatocytes, astrocytes, epithelial cells, fibroblasts, stem cells, and many other cell types. Selecting a kit specifically designed for the target cell type is essential for maintaining normal cellular function and morphology.
Serum-containing vs. serum-free formulations
Traditional kits often include FBS as a source of growth factors and nutrients. Serum-free and xeno-free kits replace animal-derived components with defined supplements, reducing variability and supporting applications that require animal-component-free culture conditions.
Complete kit convenience
Most kits provide all major components needed to prepare complete culture medium. This eliminates the need to source multiple reagents individually and helps ensure that supplements are used at the correct concentrations.
Antibiotic inclusion
Many primary cell culture kits include antibiotics to reduce contamination risk during routine culture. However, some specialized formulations, particularly those designed for immune cells and mononuclear cells, are intentionally supplied without antibiotics because these additives can influence cellular responses and experimental outcomes.
Storage and preparation
Kit components are typically stored separately until needed. Basal media and supplements are usually refrigerated, while serum components may require frozen storage before use. Once mixed into complete medium, shelf life is generally shorter than that of the individual components, making it advisable to prepare only the amount needed for near-term use.
Specialized differentiation systems
Some kits are designed not only for maintenance but also for cellular differentiation. These systems may include multiple supplements added sequentially throughout a protocol to guide stem cells or progenitor cells through defined developmental stages.
<h2 data-section-id="dydsco" data-start="3131" data-end="3173">&nbsp;</h2>
<h2 data-section-id="1fljvas" data-start="4061" data-end="4086">Specifications context</h2>
&nbsp;
Primary cell culture medium kits are commonly supplied in a format consisting of a basal medium bottle accompanied by concentrated supplements and serum components that are added directly before use. This approach simplifies preparation while ensuring that all components have been optimized and tested together.
<a href="https://mbpinc.net/contact-us" title="Contact the expert team at MBP">Contact the expert team at MBP</a> today and get optimized primary cell culture medium kits for your lab.

Primary Cell Culture Medium kits

Expansion Medium Kit for Fusion Hepatocyte-like Cells

Unit Quantity

Sterility

Appearance

Disclaimer

<h2>What are plasmid maxiprep kits?</h2>
&nbsp;
A plasmid maxiprep kit scales SDS/alkaline lysis with silica-membrane spin columns, anion-exchange resin with gravity flow, or fully automated cartridge-based processing to 100-500 ml of overnight E. coli culture, recovering up to 400 ug per spin-column prep or 1-1.4 mg with high-yield anion-exchange chemistry, completable in 35-75 minutes depending on format. Related entities include EndoFree-type endotoxin removal for transfection, automated processors that eliminate manual centrifugation, and downstream applications including large-scale transfection, in vitro transcription/translation, and multiple parallel experiments. Choose Maxiprep when your application needs the largest routine plasmid yields (hundreds of micrograms to over 1 mg) from a single overnight culture.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Ultrafast 25-minute maxiprep kits drastically reduce processing times using specialized column technology&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Syringe-filter plasmid maxi units rapidly clear dense bacterial lysates using vacuum-free clarification systems&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid maxiprep systems for delivering transfection-grade DNA in sensitive mammalian cell lines</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-performance silica-column vectors optimized for maximum concentration and recovery of low and high-copy plasmids</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Gravity-flow anion-exchange assemblies without manual centrifugation steps, ensuring chromatography-level purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid maxiprep kit</h2>
&nbsp;
When maxiprep is the right scale
A maxiprep should be used when purifying plasmid from an overnight bacterial culture with a volume between 100-500 ml, when downstream needs exceed midiprep's 0.5-2 mg range - for example, large-scale transfection across many plates, in vitro transcription/translation requiring substantial template DNA, or banking DNA for multiple future experiments from one culture. If midiprep yields already meet your needs, the larger culture volume and processing time of maxiprep may not be justified.
Spin-column vs. anion-exchange vs. automated chemistry
Spin-column maxipreps (e.g., 60-minute procedures) recover up to roughly 400 ug per prep, while anion-exchange or high-yield chemistries can reach 1-1.4 mg from comparable culture volumes; automated cartridge-based processors can deliver endotoxin-free DNA in under 75 minutes without manual centrifugation, pouring in culture, and pressing start. Match chemistry to both your yield target and available equipment - automated processors reduce hands-on time but represent a larger equipment investment.
Endotoxin levels at maxi scale
Endotoxin concentrations tend to be higher in larger-scale preparations - standard maxipreps have been reported at 11-16 EU/ug, while EndoFree-type kits target below 0.1 EU/ug through additional washing, and "substantially endotoxin-free" is sometimes defined as under 100 EU/ug for less stringent applications. For transfection of highly sensitive cells, vaccination research, or gene therapy applications, confirm the maxiprep kit's specific endotoxin target rather than assuming "maxiprep" implies low endotoxin by default.
Lysate clearing and centrifuge requirements
Maxi-scale lysate clearing typically requires high-speed centrifugation of the full culture volume (100-500 ml) followed by a clearing column or filtration step; gravity-flow anion-exchange systems use folded filters to avoid this centrifugation, while automated processors handle clearing internally. Confirm your centrifuge has rotors compatible with 100-500 ml culture volumes before committing to a centrifugation-based maxiprep format.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for maxiprep selection are expected yield (400 ug for spin-column vs. 1-1.4 mg for high-yield/anion-exchange chemistry), endotoxin target relative to your transfection application's sensitivity, and lysate-clearing method relative to your centrifuge's capacity for 100-500 ml culture volumes. A maxiprep should specifically be used for culture volumes in the 100-500 ml range; smaller volumes are better served by <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;or&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>. Labs running large-scale transfection or in vitro transcription/translation programs typically standardize on either high-yield anion-exchange maxipreps or automated processors to balance yield, endotoxin level, and hands-on time. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;covers the full mini-to-maxi range, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a>&nbsp;link to chromosomal DNA workflows. Use MBP's&nbsp;<a href="https://mbpinc.net/quickorder">Quick Order tool</a>&nbsp;for case-quantity pricing on maxiprep kits or&nbsp;<a href="https://mbpinc.net/contact-us">contact the specialist team</a>&nbsp;for guidance on endotoxin levels and automation options for your transfection program.
<section class="mbp-contact-cta"></section>
&nbsp;
Scale-up workflow efficiency with MBP&rsquo;s dependable macro-extraction plasmid technologies. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> today to find the perfect match for your lab.

Maxiprep Kits

User Manual

Express Endofree Plasmid Maxiprep

Express Endofree Plasmid Maxiprep (PD2568-00) by MBP is a maxi-scale endofree plasmid DNA purification kit. Available in 2 (PD2568-00), 10 (PD2568-01), and 25 (PD2568-02) prep sizes. Refer to User Manual for protocol details.

Express Endofree Plasmid Maxiprep, 2 Preps

Express Endofree Plasmid Maxiprep, 10 Preps

Express Endofree Plasmid Maxiprep, 25 Preps

<h2>What are plasmid midiprep kits?</h2>
&nbsp;
A plasmid midiprep kit scales SDS/alkaline lysis and silica-membrane column binding (or anion-exchange resin with gravity flow) to 150-300 ml of overnight E. coli culture, recovering 0.5-2 mg of plasmid DNA with lysate clearing accomplished by high-speed centrifugation followed by a clearing column or filtration step, completable in as little as 30 minutes for some spin/vacuum formats. Related entities include high-speed centrifugation for lysate clearing, NucleoSnap-type midi formats, endotoxin removal washes, and downstream applications including moderate-scale transfection and multi-reaction restriction/sequencing work. Choose midiprep when miniprep yields (up to ~25-100 ug) aren't sufficient, but full maxiprep-scale culture volume (100-500 ml) and processing time aren't necessary.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Rapid 25-minute midiprep kits to bypass lengthy gravity-flow precipitation steps utilizing accelerated column dynamics</li>
<li dir="ltr" role="presentation">Vacuum-assisted syringe-filter plasmid midi modules engineered for rapid and debris-free clearing of lysates without manual pelleting</li>
<li dir="ltr" role="presentation">Endotoxin-free intermediate purification systems for sensitive mammalian cell screening by delivering transfection-grade constructs</li>
<li dir="ltr" role="presentation">High-purity silica spin-column configurations optimized for elution of high-copy vectors&nbsp;</li>
<li dir="ltr" role="presentation">Advanced anion-exchange gravity-flow assemblies provide template purity for downstream sequencing.</li>
</ul>
&nbsp;
<h2>How to choose a plasmid midiprep kit</h2>
&nbsp;
When midiprep fits between mini and maxi
Midiprep format is the right choice when your downstream application needs more DNA than a miniprep provides (roughly 20-100 ug depending on chemistry) but doesn't require the largest maxiprep-scale yields (up to 1 mg or more); 0.5-2 mg from 150-300 ml culture covers many moderate-scale transfection experiments and multi-replicate downstream reactions. If you find yourself running multiple minipreps to pool DNA for a single transfection, a single midiprep is often more efficient.
Lysate clearing method
Midiprep kits clear lysate using high-speed centrifugation followed by a clearing column (spin or vacuum format) or filtration through folded filters in anion-exchange systems; spin/vacuum clearing formats can complete the full protocol in as little as 30 minutes, while gravity-flow anion-exchange systems may take longer but avoid centrifugation steps for the binding/wash/elute portion. Match the clearing method to your lab's available centrifuge capacity (rotor size for 150-300 ml tubes) and time constraints.
Endotoxin-free midiprep options
For transfection of sensitive cell types, endotoxin-free midiprep kits using anion-exchange chemistry can achieve endotoxin removal in a unique washing step without additional incubations, suitable for transfection of highly sensitive cells, vaccination research, or gene therapy applications. If your midi-scale prep feeds directly into sensitive-cell transfection, confirm the kit's endotoxin specification (e.g., below 0.1 EU/ug) against your cell type's documented sensitivity.
Plasmid copy number and culture volume within the midi range
As with miniprep, plasmid copy number affects yield within the midiprep culture volume range - low-copy plasmids may need culture volumes toward the higher end (300 ml) to reach typical midi yields, while high-copy plasmids may reach 1-2 mg even at 150 ml. Plan culture volume within the midi range based on your specific vector rather than defaulting to the kit's maximum stated volume.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for midiprep selection are whether 0.5-2 mg from 150-300 ml culture meets your downstream DNA quantity needs without over- or under-scaling, lysate clearing method (centrifugation-based spin/vacuum vs. gravity-flow anion-exchange), and endotoxin status for transfection-sensitive applications. A midiprep completing in as little as 30 minutes via spin/vacuum clearing offers a time advantage over gravity-flow systems while still avoiding the larger culture volumes of maxiprep. Labs whose miniprep yields are consistently insufficient for their transfection protocols typically move to <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;before considering&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>, which require substantially more culture volume and processing time. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;cover smaller-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Midiprep Kits

Express Endofree Plasmid Midiprep

Express Endofree Plasmid Midiprep (PD2468-00) by MBP is a midi-scale endofree plasmid DNA purification kit. Available in 2 (PD2468-00), 10 (PD2468-01), and 25 (PD2468-02) prep sizes.

Express Endofree Plasmid Midiprep, 2 Preps

Express Endofree Plasmid Midiprep, 10 Preps

Express Endofree Plasmid Midiprep, 25 Preps

Express Plasmid Maxiprep Kit (25 min)

Express Plasmid Maxiprep Kit (PD1568-00) by MBP is a maxi-scale plasmid DNA purification kit with a 25-minute protocol. Available in 2 (PD1568-00), 10 (PD1568-01), and 25 (PD1568-02) prep sizes.

Express Plasmid Maxiprep Kit (25 min), 2 Preps

Express Plasmid Maxiprep Kit (25 min), 10 Preps

Express Plasmid Maxiprep Kit (25 min), 25 Preps

Product Literature

Express Plasmid Midiprep Kit ( 25 min)

Express Plasmid Midiprep Kit (PD1468-00) by MBP is a midi-scale plasmid DNA purification kit with a 25-minute protocol. Available in 2 (PD1468-00), 10 (PD1468-01), and 25 (PD1468-02) prep sizes.

Express Plasmid Midiprep Kit ( 25 min), 2 Preps

Express Plasmid Midiprep Kit ( 25 min), 10 Preps

Express Plasmid Midiprep Kit ( 25 min), 25 Preps

Dry Baths Accessories

<h2>What Are Dry Bath Incubator Accessories?</h2>
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Dry bath accessories are the secondary components that extend or optimize the functionality of a dry block heater: aluminum insert blocks that define the tube format capacity; heated lids that eliminate condensation; external temperature reference probes for calibration verification; and carrying and storage cases for portability. Because the aluminum insert block physically determines which tube formats the dry bath can process, insert block selection is the single most impactful purchase decision after the base unit itself. Most manufacturers supply one insert format as standard with the base unit; labs processing multiple tube formats must purchase additional inserts separately. Select accessories based on the tube formats used in your primary protocols, confirming that inserts are brand- and model-compatible before ordering.
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<h2 dir="ltr">What You Will Find:</h2>
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Specialized Heating Blocks: High-conductivity aluminum blocks tailored for everything from 0.2ml PCR strips and 1.5ml centrifuge tubes to larger 50ml conical vessels.
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Custom &amp; Solid Block Options: Versatile solid blocks suitable for slides or custom machining, alongside blocks specifically drilled for HPLC vials and cuvettes.
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Precision Monitoring &amp; Power: External temperature probes for direct sample tracking and 12V vehicle adapters to maintain thermal control during mobile laboratory work.
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<h2>How to Choose Dry Bath Accessories</h2>
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Aluminum insert blocks: format selection
Insert blocks are precision-machined from aluminum to accept specific tube formats with tight well tolerances (typically within plus or minus 0.1 mm of the tube's outer diameter). Common formats include: 24 wells for 0.5 mL tubes; 15-16 wells for 1.5 mL microcentrifuge tubes; 12-15 wells for 2 mL microcentrifuge tubes; 8-strip configuration for 0.2 mL PCR strip tubes; 12 wells for 15 mL conical tubes; 6 wells for 50 mL conical tubes; and a flat surface (no wells) for microplates or irregular vessels. Inserts are not universally interchangeable across brands; confirm the insert fits the specific dry bath model's block holder dimensions and, for models with auto-recognition, that the insert carries the required auto-recognition contact.
Insert material and thermal conductivity.
Aluminum (Al 6061 alloy) is the standard material for dry bath inserts due to its high thermal conductivity (approximately 167 W/m K), low density, and machinability for tight tolerances. Some specialty inserts for aggressive chemical applications use hard-anodized aluminum or stainless steel, with lower thermal conductivity but better chemical resistance. Always use manufacturer-supplied or manufacturer-approved aluminum inserts; after-market inserts with inferior tolerances reduce thermal contact and degrade temperature uniformity.
Heated lid accessories
Heated lid accessories for dry baths are sold separately for many base unit models or as an upgrade kit. When selecting a heated lid, confirm: (1) it fits the specific block footprint and height of your dry bath model; (2) the lid temperature range covers the required anti-condensation temperature (typically 60-110 degrees C); (3) the lid temperature is independently controlled from the block; and (4) the lid surface material is non-reactive with samples. For protein and enzyme protocols, a lid temperature 5-10 degrees C above the block temperature is the typical starting point.
External temperature reference probe
An external calibration probe (typically a thin RTD or thermocouple probe rated for direct insertion into a tube well, connected to a calibrated digital reference thermometer) verifies that the temperature inside the well matches the instrument's displayed setpoint. External probe verification is required for GLP-compliant and regulated laboratory protocols (ISO 9001, CLIA, cGxP) and is good practice annually for any research dry bath. Confirm that the probe diameter fits the insert well format (most probes are 2-5 mm diameter; well diameter varies by tube format) before ordering.
Carrying case for portable dry baths
Mini and portable dry bath models (Labnet PR1MA Mini, and similar) are available with dedicated carrying cases for field applications: point-of-care diagnostics, on-site environmental sampling, and field isothermal PCR or LAMP assays using a 12 V DC automotive power adapter. Carrying cases provide impact protection, secure block holder positioning, and cable management for field use.
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<h2>Specifications Context</h2>
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Aluminum insert block well tolerances of plus or minus 0.1 mm ensure full circumferential contact between the tube outer wall and the block well, maximizing heat transfer and minimizing temperature gradients between the sample and the block setpoint. A loose-fitting insert with 0.5 mm or more of air gap between tube and well reduces heat transfer and can introduce a temperature lag of 1-3 degrees C compared to the block setpoint, systematically underheating samples. Heated lid accessories eliminate condensation-related volume errors of 5-15% that can occur in short (&lt;10 microliter) enzyme reactions at 37-55 degrees C over 30-60 minute incubations. Auto-recognized interchangeable block inserts (where the dry bath unit automatically detects which format insert is installed) are a standard feature on Labnet and similar mid-range dry bath models, improving setup speed and reducing configuration errors.&nbsp;
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Enhance your dry bath setup today&mdash;check out our full range of blocks and <a href="https://mbpinc.net/contact-us">reach out to the MBP team</a> for a quote.

External Temperature Probe

The BSH-TP1 by Benchmark Scientific is an external temperature probe for the myBlock Mini (BSH200) and IsoBlock dry baths. It monitors actual sample temperature inside the tube and feeds the reading to the control circuit, enabling sample-level temperature regulation rather than block surface control alone.

Externally Threaded Cryogenic Vials

<h2 data-section-id="17lohz5" data-start="0" data-end="48">What Are Externally Threaded Cryogenic Vials?</h2>
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Externally threaded cryogenic vials have the screw thread on the outside of the vial neck, with a cap that covers the entire top portion of the vial when closed. Manufactured from cryogenic-grade virgin polypropylene, these vials are designed to withstand temperatures from &minus;196&deg;C to +121&deg;C and are commonly used for long-term storage of cell lines, serum, blood, DNA, RNA, bacterial cultures, and other biological samples. Available in volumes ranging from 1.2 mL to 5 mL, they are offered in both self-standing and round-bottom formats. Unlike internally threaded vials, external-thread designs use an integrated sealing system rather than a separate O-ring, helping reduce handling issues. For automated decapping systems, high-throughput biobanking, and SBS-format robotic workflows, externally threaded vials are the preferred format.
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<h2 dir="ltr">MBP Offers:</h2>
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2D Barcoded Cryogenic Vials for automated tracking
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SBS Format Cryo Racks for robotic integration
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3D Barcoded Storage Vials for digital inventory
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Color-coded Cap Inserts for rapid identification
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Self-standing Star-foot Cryo Vials
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Silicone O-ring Sealed Leak-proof Containers
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Gamma-sterilized Aseptic Storage Tubes
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&nbsp;
<h2 data-section-id="px7lzo" data-start="891" data-end="943">How to Choose Externally Threaded Cryogenic Vials</h2>
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Volume
The 2 mL externally threaded vial is the industry standard for cell banking, serum aliquots, and general biospecimen storage. It fits standard 81-well cryo boxes and is suitable for most laboratory applications. Smaller 1.2&ndash;1.5 mL vials are ideal for high-density storage where space efficiency is important, while larger 3&ndash;5 mL formats are commonly used for tissue samples, bacterial glycerol stocks, and larger-volume specimens.
Base Style: Self-Standing vs. Round Bottom
Self-standing vials feature a star-foot or conical base that allows them to stand upright on laboratory benches without requiring a rack, reducing the risk of spills during handling. Round-bottom vials require rack support but often offer broader compatibility with different storage racks and cryo box configurations.
Barcoding: Plain vs. 2D Barcoded
Standard externally threaded vials include a writable surface for manual labeling and are suitable for small to medium-sized sample collections. For large biobanks and automated sample management systems, 2D barcoded vials provide Data Matrix codes that can be scanned directly into Laboratory Information Management Systems (LIMS), improving traceability and reducing manual data entry errors.
Sterility and Purity
Cryogenic vials intended for cell culture, molecular biology, and clinical research should be gamma sterilized and certified RNase-free, DNase-free, non-pyrogenic, and endotoxin-free. These certifications help protect sample integrity and reduce contamination risks during long-term storage.
&nbsp;
<h2 data-section-id="1jl751w" data-start="3061" data-end="3086">Specifications Context</h2>
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Externally threaded polypropylene cryogenic vials are designed for vapor-phase liquid nitrogen storage and ultra-low temperature freezers. The standard outer diameter of a 2 mL vial is approximately 12.5 mm, making it compatible with most 81-well cryo box formats. Modern external-thread designs use integrated thermoplastic elastomer sealing systems that eliminate the need for separate O-rings, reducing the possibility of seal misalignment or loss during routine handling. As of 2026, externally threaded 2 mL vials with SBS-compatible footprints and 2D barcodes are widely used in large-scale biobanking and sample repository applications.
Your research is priceless. Your storage should be equally reliable. <a href="https://mbpinc.net/contact-us" style="color: #236fa1; text-decoration: underline;">Connect with Molecular Biology Products Inc.</a> for a personalized quote.

Datsheet

External Thread Self-Standing Cryogenic Vial, Sterile, New Model, Box

606901 by NEST is a sterile self-standing external-thread cryogenic vial made from USP Class VI polypropylene, rated to −196 °C in liquid nitrogen vapor phase. Certified DNase-free, RNase-free, human DNA-free, non-pyrogenic. Manufactured under cGMP, ISO 13485, and ISO 9001. Sold in box format.

1.5 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 50/pk, 500/box, 2000/cs

2.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 50/pk, 500/box, 2000/cs

4.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 50/pk, 250/box, 1000/cs

5.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 50/pk, 250/box, 1000/cs

1.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 96/pk, 960/cs

External Thread Self-Standing Cryogenic Vial, Sterile, New Model, Racked

606902 by NEST Scientific is a sterile, self-standing, external-thread cryogenic vial in racked format (10×10, 100 vials/rack). USP Class VI virgin polypropylene, rated to −196 °C (vapor-phase LN₂) and autoclavable to 121 °C. E-beam sterilized, DNase/RNase-free, human DNA-free, non-pyrogenic. Multiple volumes available.

1.5 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 10*10/rack, 14 racks/cs, 1400 vials/cs

2.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 10*10/rack, 12 racks/cs, 1200 vials/cs

4.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 10*10/rack, 8 racks/cs, 800 vials/cs

5.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 10*10/rack, 6 racks/cs, 600 vials/cs

1.0 mL Cryogenic Vial, Self-Standing, External Thread, Sterile, New Model, 12*8/rack, 10 racks/cs, 960 vials/cs

139030-Group-Transfer Pipet Brochure

139030-Group-TDS-139050

Extra Long Transfer Pipets Specifications

Extra Long Transfer Pipets

The 139030 by Globe Scientific is a 6.0 mL extra-long transfer pipet — 225 mm (9-inch) stem, 2.3 mL bulb draw, 4.6 mm stem diameter — designed for aspirating from 24-hour urine containers, large reagent bottles, cylinders, and jars. Non-sterile bulk format: 500 per box. Sterile variant (139030-S20) ships 20 per bag, 400 per case.

Transfer Pipet, 6.0mL, Extra Long, 225mm (9 Inches Long), 500/Dispenser Box, 10 Boxes/Unit

Transfer Pipet, 6.0mL, Extra Long, 225mm (9 Inches Long), 500/Dispenser Box

Transfer Pipet, 6.0mL, Extra Long, 225mm (9 Inches Long), STERILE, 20/Bag, 20 Bags/Unit

Transfer Pipet, 6.0mL, Extra Long, 225mm (9 Inches Long), STERILE, Individually Wrapped, 100/Pack, 4 Packs/Unit

Transfer Pipet, 23.0mL, Extra Long, 300mm (12 Inches Long), 100/Box

Transfer Pipet, 23.0mL, Extra Long, 300mm (12 Inches Long), 100/Box, 10 Boxes/Unit

Transfer Pipet, 23.0mL, Extra Long, 300mm (12 Inches Long), STERILE, Individually Wrapped, 100/Unit

Transfer Pipet, 23.0mL, Extra Long, 300mm (12 Inches Long), STERILE, 20/Bag, 5 Bags/Unit

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