Zymo Research

<h2>What are Magnetic Bead Total RNA Extraction Kits?</h2>
&nbsp;
Magnetic bead total RNA extraction kits isolate total RNA by adsorbing nucleic acids onto paramagnetic silica-coated beads in a high-chaotropic-salt lysis buffer, then using a magnetic field to capture and wash beads without centrifugation. Unlike standard spin-column kits, the bead format removes centrifuge dependency, enabling scalable manual processing in 96-well plates or full automation on liquid-handling robots.&nbsp;Choose a magnetic bead format when your lab processes more than 48 samples per day or when a Tecan, Hamilton, or Beckman Biomek platform is available.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Kits for liquid biopsy collection systems and non-invasive swabbing using rapid magnetic bead isolation&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Zymo-Seq RiboFree&trade; Kits to eliminate ribosomal RNA carryover during library synthesis
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Direct-zol-96 MagBead RNA Modules facilitate complete avoidance of manual phase-separation steps while enabling automated transcript extraction directly from TRIzol&reg; lysates&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Magbio HighPrep Total RNA Plus Kit extracts pure, inhibitor-free RNA from complex samples with maximum binding capacity&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA MagBead Packs deliver high-integrity total transcripts without dependence on centrifuge spins or vacuum manifold.
</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead Total RNA Extraction Kit</h2>
&nbsp;
Automation Compatibility
Confirm that the kit supplier provides automation scripts for your specific platform. The Zymo Research Quick-DNA/RNA Viral MagBead kit and Thermo Fisher MagMAX-96 both offer downloadable instrument scripts for Tecan and Hamilton systems. Generic magnetic bead kits without published automation protocols require in-house script development, adding significant setup time.
Small RNA Retention
Bead kits that bind RNA &gt;=17 nt co-purify microRNAs alongside mRNA and rRNA from the same input. Kits specifying &gt;=200 nt retention exclude small RNAs and are not appropriate for miRNA expression panels. Check the size cutoff stated in the kit datasheet before selecting for non-coding RNA work.
Input Sample Type
Magnetic bead kits for total RNA support cell pellets, tissue homogenates, blood, bacteria, yeast, and fungi. For cell-free matrices (plasma, serum, nasal wash), switch to a viral-RNA or liquid-biopsy magnetic bead kit with included carrier RNA to maximize recovery from low-input, cell-free samples.
Elution Volume and Concentration
Standard magnetic bead kits elute into 50-100 ul; some protocols allow concentration into 20-50 ul, increasing RNA concentration for sensitive downstream applications. If downstream libraries require &gt;=200 ng/ul input, reduce elution volume accordingly and verify the kit supports this protocol variant.
gDNA Removal
Confirm whether the kit includes on-bead DNase I or requires a separate gDNA digestion step. For RT-qPCR and RNA-seq, omitting DNase treatment introduces a genomic DNA signal that inflates expression measurements.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
RNA purity targets from magnetic bead extractions match spin-column benchmarks: A260/A280 &gt;= 1.8 and A260/A230 &gt;= 1.5. RNA integrity number (RIN) &gt;= 7 is recommended for RNA-seq library preparation; magnetic bead kits produce RIN values in the 8-10 range for fresh samples when lysis is performed immediately on ice. Bead-based workflows are cited in published automation protocols from genomics core facilities and are increasingly specified by NIH-funded projects requiring standardized sample processing across multi-site studies.
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP Inc. to get automation-compatible mag bead selections today.

Mag Beads

MSDS

DataSheet

Protocol

Quick-RNA MagBead

Quick-RNA™ MagBead R2132 by Zymo Research — 96-prep magnetic bead RNA isolation, RNA ≥17 nt (including miRNA), 15 µg per 30 µL beads, ≥50 µL elution. Inputs: cells, tissue, whole blood, FFPE, environmental, swabs, and DNA/RNA Shield samples. Compatible with liquid handlers and robotic processors; ZR-96 MagStand (P1005) recommended. Also available in a 4 &times; 96 prep format (R2133). Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Binding Capacity

Equipment Needed

Purity

Recommended Materials

Sample Preservation

Sample Sources

Size Limits

Storage

Manufacturer

Shipping Condition

Storage Condition

Quick-RNA™ MagBead (96 Preps.)

Quick-RNA™ MagBead (4 x 96 Preps.)

<h2>What Are Spin-Column Total RNA Kits?</h2>
&nbsp;
Spin-column total RNA kits are the most widely used format for manual RNA purification in molecular biology laboratories. The technology relies on silica membrane chemistry: under chaotropic (guanidinium-based) lysis conditions, RNA adsorbs selectively to the silica membrane of the column; sequential ethanol-based wash steps remove proteins, salts, and gDNA fragments; and a low-ionic-strength buffer or RNase-free water then releases the purified RNA. Standard mini-format kits accept cell or tissue lysate from inputs of 1x10^4-1x10^7 cells or 5-100 mg tissue, bind up to 100 ug RNA, and complete the protocol in 15-30 minutes. The eluted RNA is suitable for RT-PCR, qRT-PCR, RNA sequencing, Northern blot, and hybridization without additional cleanup.&nbsp;Spin-column RNA kits are the default choice for 1-24 samples per run in any lab that does not require automated magnetic-bead processing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-RNA Microprep and Miniprep Kits for low-volume elution and highly concentrated recovery, providing ultra-clean and phenol-free transcript isolation
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; RNA Kits to bypass tedious phase separation steps, allowing researchers to pump TRIzol-lysed samples directly into spin-columns&nbsp;
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; DNA/RNA Parallel Modules are engineered for the non-destructive, simultaneous isolation of DNA and RNA fractions.
</li>
<li dir="ltr" aria-level="1">
ZymoBIOMICS&trade; RNA Mini Kits for uniform microbial profiling featuring host-zeroing lysis mechanics
</li>
<li dir="ltr" aria-level="1">
Quick-RNA&trade; Midiprep Assemblies to capture larger quantities of high-purity total transcripts efficiently, tailored for bulk processing&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose Spin-Column Total RNA Kits</h2>
&nbsp;
Column Binding Capacity and Elution Volume
Mini-format columns bind up to 100 ug total RNA and elute in 10-100 uL. For samples with very low RNA content (needle biopsies, laser-capture microdissection sections, small cell numbers &lt;=10^4), choose micro-format columns with 8-12 uL minimum elution volume to maximize concentration. Eluting in &lt;30 uL with high RNA concentration increases sensitivity in downstream RT-qPCR.
Small RNA Retention
This is the most important differentiator between kit brands. Kits using Zymo-Spin column technology with proprietary binding buffers retain miRNA and small RNA species &gt;=17 nt in the total RNA fraction. Competing kits using standard high-ethanol wash chemistry typically lose RNA &lt;200 nt in the wash steps. If your workflow includes small RNA profiling, miRNA RT-qPCR, or small RNA-seq, verify that the kit retains &gt;=17 nt RNA before purchasing.
On-Column DNase Treatment
Included RNase-free DNase I that degrades gDNA on the column during the wash stage eliminates the need for post-elution DNase treatment. This matters for RT-qPCR with primers spanning a small intron, or for RNA-seq where even trace gDNA contamination generates false mapping reads. DNase-treated RNA typically passes a no-RT PCR control at Ct &gt;35.
Sample Type: Cells vs Tissue
Cell lysis buffer volumes and homogenization requirements differ by sample type. Cultured cells lyse directly in lysis buffer with vortexing; tissue samples require homogenization to disrupt cell structure before contact with the lysis buffer. For fibrous tissues (muscle, heart), use a bead-mill homogenizer or rotor-stator; for soft tissues (liver, brain), a disposable pestle or needle shearing is sufficient. Some kits include TissueLyser-compatible bead tubes or QIAshredder-equivalent homogenizer columns.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Spin-column total RNA purity targets: A260/A280 ratio 1.8-2.1; A260/A230 ratio &gt;1.8. RNA integrity number (RIN) from fresh cultured cells or snap-frozen tissue is routinely 8-10 with validated kits. Zymo Research Quick-RNA Mini and Miniprep kits are among the most cited spin-column RNA isolation kits in published RT-PCR and RNA-seq methods; ABM RNA extraction kits offer validated protocols for bacteria, yeast, and fungi not covered by many standard cell/tissue kits. All kits supplied by MBP are Research Use Only (RUO).
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Spin-column

Quick-RNA Microprep Kit

Quick-RNA™ Microprep Kit R1050 by Zymo Research isolates total RNA ≥17 nt — including miRNA — from cells, tissue, yeast, plant, and bacteria using Zymo-Spin IC columns, with ≥6 µL elution and on-column DNase I digestion. Compatible with DNA/RNA Shield and RNAlater; lysed samples stable overnight at RT or at –80°C. Available in 50-prep (R1050) and 200-prep (R1051) formats from MBP via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Equipment

Sample Source

Size Range

Yield

Quick-RNA™ MicroPrep Kit (50 Preps.)

Quick-RNA™ MicroPrep (200 Preps.)

Quick-RNA MiniPrep Kit

Quick-RNA MiniPrep Kit by Zymo Research — total RNA isolation in ~10 min with up to 100 µg binding capacity, A260/A280 &gt;1.8, RNA ≥17 nt. DNase I included. Available as R1054 (50 Preps) and R1055 (200 Preps).

Quick-RNA™ MiniPrep Kit (50 Preps.)

Quick-RNA™ MiniPrep Kit (200 Preps.)

MSDS - R1057/R1058

MSDS - R1057T

Quick-RNA Miniprep Plus Kit

The Quick-RNA™ Miniprep Plus Kit by Zymo Research isolates total RNA — including small RNAs ≥17 nt — from cells, all tissue types, whole blood, FFPE samples, and biological fluids. Zymo-Spin IIICG columns with integrated spin-away filters; DNase I and DNA/RNA Shield included. Typical yield 10–15 µg per 10⁶ cells; purity A260/A280 &gt;1.8. Available as R1057T (10 preps), R1057 (50 preps), and R1058 (200 preps).

Quick-RNA™ MiniPrep Plus Kit (50 Preps.)

Quick-RNA™ MiniPrep Plus (10 Preps.)

Quick-RNA™ MiniPrep Plus Kit (200 Preps.)

<h2>What are 96-Well Plate Viral RNA Extraction Kits?</h2>
&nbsp;
96-well plate viral RNA extraction kits purify viral RNA from cell-free biological matrices - plasma, serum, nasopharyngeal swabs, urine, cerebrospinal fluid, saliva, and cell culture supernatant - processing all 96 wells simultaneously using silica-membrane spin plates or magnetic bead 96-well formats compatible with centrifuges, vacuum manifolds, or liquid-handling robots. Unlike single-tube spin-column viral RNA formats that handle 1-24 samples per run, 96-well plate kits deliver the throughput required for batch diagnostic testing, surveillance programs, and large-scale biobank processing.&nbsp;Use a 96-well plate viral RNA kit when processing 48 or more samples per day from cell-free matrices where viral genome copy numbers may be low.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Quick-RNA&trade; Viral 96 Kit for rapid, large-scale isolation of viral RNA from plasma, serum, saliva, urine, blood, cell culture media, and cellular suspensions.</li>
<li>High-throughput 96-well spin-plate format for simultaneous processing of large sample batches.</li>
<li>Compatible with samples preserved in DNA/RNA Shield for nucleic acid stabilization during storage and transport at ambient temperatures.</li>
<li>Specialized lysis chemistry designed for efficient recovery of viral RNA from a broad range of RNA and DNA viruses.</li>
<li>Purification workflows that generate high-quality viral RNA suitable for RT-PCR, qPCR, next-generation sequencing, and hybridization-based detection methods.</li>
<li>Streamlined spin-plate extraction technology that supports efficient binding, washing, and elution of viral nucleic acids.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a 96-Well Plate Viral RNA Extraction Kit</h2>
&nbsp;
Spin-Plate vs. Magnetic Bead 96-Well Format
Silica-membrane spin-plate kits (e.g., Zymo Quick-RNA Viral 96, Thermo PureLink Pro 96) use centrifugation or vacuum manifold and complete extraction in approximately 30 minutes without a robot. Magnetic bead 96-well formats eliminate centrifugation and integrate with KingFisher Flex, Tecan, and Hamilton platforms; choose beads when a compatible robot is available, and daily volume exceeds 192 samples.
RNA-Only vs. DNA/RNA Co-Extraction
Standard viral RNA spin-plate kits isolate RNA targets. If your assay panel includes both RNA viruses (influenza, SARS-CoV-2, RSV) and DNA viruses (adenovirus, herpesvirus, parvovirus), select a dual-format kit - the Zymo Quick-DNA/RNA Viral 96 Kit co-extracts both from the same 100-200 ul input using a single-buffer lysis system.
Virus Validation List
Confirm the kit's validation data covers your target virus family before adoption. The Zymo Quick-RNA Viral 96 Kit lists validation across coronaviruses, influenza A, flaviviruses, enteroviruses, HIV, HCV, and parvovirus. Kits without published validation for your specific target require in-house validation against a quantified reference standard.
Carrier RNA and Lysis Chemistry
Low-titer specimens benefit from carrier poly(A) RNA in the lysis buffer. Kits using beta-mercaptoethanol as a deproteinization agent improve yield from protein-rich plasma and serum; it is not necessary for simple swab samples. Confirm lysis chemistry matches your sample matrix.
Throughput Beyond 96
For workflows requiring more than 96 extractions per batch, confirm that the kit vendor's plate format is stackable or that the kit supports same-session re-processing without reagent waste.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for 96-well plate viral RNA kits: &gt;=70% extraction efficiency by spike-in control, well-to-well Ct variability &lt;=1 cycle, and A260/A280 &gt;= 1.7 in eluates. Standard elution volumes of 50-100 ul per well are suitable for most RT-qPCR assays requiring 2-5 ul input.96-well plate viral RNA extraction is the standard format in accredited clinical microbiology laboratories processing SARS-CoV-2, influenza A/B, and RSV diagnostic panels at volume; workflows built around this format are referenced in published biosafety and QC guidance from institutions, including the Howard Hughes Medical Institute. RNase-free consumables and lysis buffers that inactivate infectious agents at the point of lysis are required for safe batch processing of potentially infectious clinical material.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

96-well plate

R1040 - MSDS

R1040-Protocol

Quick-RNA Viral 96 Kit

Quick-RNA Viral 96 Kit by Zymo Research is a 96-well spin plate purification system that binds 10 µg RNA per well and elutes in ≥10 µL, recovering 50 nt–200 kb viral RNA from plasma, serum, CSF, swabs, urine, blood, and other sample types — without organic denaturants or proteases. CE-IVD formats available.

Registration Status

Quick-RNA™ Viral 96 Kit (2 x 96 Preps.)

Quick-RNA™ Viral 96 Kit - DX (2 x 96 Preps.)

Quick-RNA™ Viral 96 Kit (4 x 96 Preps.)

Quick-RNA™ Viral 96 Kit - DX (4 x 96 Preps.)

<h2>What are Spin-Column Viral RNA Extraction Kits?</h2>
&nbsp;
Spin-column viral RNA extraction kits isolate RNA from RNA viruses found in cell-free biological fluids by passing the sample through a silica-membrane column in a standard microcentrifuge. A chaotropic lysis buffer simultaneously disrupts viral envelopes, inactivates RNases, and conditions the RNA to bind the silica membrane; successive wash steps remove co-purified proteins, lipids, and PCR inhibitors; and purified viral RNA elutes in 30-60 ul of RNase-free water. The full protocol takes approximately 20 minutes for batches of 1-24 samples, requiring only a microcentrifuge and standard pipetting equipment.&nbsp;Choose a spin-column viral RNA kit when processing fewer than 24 samples per run, when automation infrastructure is unavailable, or when same-day results require the fastest possible turnaround.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li>Quick-RNA&trade; Viral Kit for rapid isolation of high-quality viral RNA from a wide range of transport media and liquid samples.</li>
<li>Advanced silica-membrane spin-column technology for efficient viral RNA binding, washing, and recovery.</li>
<li>Benchtop centrifugal workflows that simplify sample processing with minimal hands-on time.</li>
<li>Viral RNA purification solutions suitable for downstream RT-PCR, qPCR, sequencing, and molecular diagnostic applications.</li>
<li>Reliable extraction of clean viral nucleic acids from clinical, research, and surveillance samples.</li>
<li>Streamlined RNA isolation protocols designed for consistent performance across diverse specimen types.</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Viral RNA Extraction Kit</h2>
&nbsp;
Single-Buffer vs. Multi-Buffer Lysis
Zymo Research Quick-RNA Viral Kits use a single lysis buffer formulation that eliminates reagent preparation steps and reduces handling time. QIAGEN QIAamp Viral RNA Mini Kits use a two-reagent approach (AVL buffer + ethanol) that requires a mixing step before loading. Single-buffer formats reduce pipetting errors in high-frequency diagnostic workflows.
Sample Input Volume
Standard spin-column viral RNA kits accept 140-300 ul per column. For low-titer applications where concentrating a larger plasma volume is needed, verify whether the kit offers extended-volume protocols. QIAGEN's QIAamp Viral RNA Mini Kit specifies 140 ul; Zymo Quick-RNA Viral Kit supports up to 50 ul in direct-column mode with optional elution concentration.
Carrier RNA Inclusion
Kits that include carrier poly(A) RNA in the lysis buffer recover 1-2 additional Ct worth of low-copy viral targets compared to kits without it. Confirm carrier RNA is included; its absence is the most common reason for inconsistent performance on low-viral-load clinical specimens.
DNA Co-Extraction Need
Standard viral RNA spin-column kits do not reliably extract dsDNA viruses. For pan-pathogen applications covering both RNA and DNA viruses from the same sample, select a dual-extraction format (Zymo Quick-DNA/RNA Viral, Thermo PureLink Viral RNA/DNA Mini).
Downstream Application
For RT-qPCR, 30 ul elution volumes concentrate RNA and increase sensitivity. For sequencing library prep requiring larger input mass from low-titer samples, elute in 50-60 ul and pool replicate extractions rather than over-reducing elution volume, which decreases total yield.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for spin-column viral RNA kits: extraction efficiency &gt;=70% recovery measured by spike-in controls, eluate A260/A280 &gt;= 1.7, and RT-qPCR Ct values within 2 cycles of reference extraction. Zymo Research Quick-RNA Viral Kit remains the most frequently referenced spin-column format in WHO and CDC laboratory guidance documents for respiratory and bloodborne virus detection. RNase-free consumables (microcentrifuge tubes, tips, collection tubes) rated for spin-column use are required to prevent RNA degradation and column contamination during extraction.
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP Inc.</a> today and get high-sensitivity spin-column purification options for your lab.

Spin-Column

MSDS (EU)

R1034-MSDS (US)

Protocol - R1034/R1035

Protocol - R1034-E/R1035-E

Quick-RNA Viral Kit

Quick-RNA™ Viral Kit by Zymo Research. Isolates viral RNA from plasma, serum, swabs, UTM/VTM, and other biological fluids. 10 µg binding capacity, ≥6 µl elution. Available in 50-prep (R1034) and 200-prep (R1035) formats, with CE-IVD DX versions (R1034-E, R1035-E).

Quick-RNA™ Viral Kit (50 Preps.)

Quick-RNA™ Viral Kit - DX (50 Preps.)

Quick-RNA™ Viral Kit (200 Preps.)

Quick-RNA Viral Kit (200 Preps.) -DX

<h2>What are FFPE RNA Extraction Kits?</h2>
&nbsp;
FFPE RNA extraction kits isolate total RNA from formalin-fixed paraffin-embedded tissue - the dominant archival format for surgical pathology specimens worldwide - using a multi-step protocol that removes paraffin, digests crosslinked proteins with proteinase K, applies heat to partially reverse formaldehyde-induced RNA crosslinks, and purifies RNA on a silica-membrane spin column. Formalin fixation crosslinks RNA to surrounding proteins through methylene bridges and fragments RNA to 100-300 bp; FFPE-specific kits are optimized for maximum recovery of this degraded, crosslinked RNA that standard tissue RNA kits cannot efficiently extract.&nbsp;Use an FFPE-specific kit whenever your starting material is a paraffin block or stained/unstained FFPE section - standard tissue RNA kits produce yields 5-20-fold lower from FFPE material than from fresh-frozen tissue.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1ci26rv" data-start="0" data-end="131">Quick-RNA&trade; FFPE MiniPrep for purification of high-quality total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples.</li>
<li data-section-id="8rfh9e" data-start="132" data-end="246">Solvent-free deparaffinization workflows that eliminate the need for hazardous xylene or other organic reagents.</li>
<li data-section-id="j7jj92" data-start="247" data-end="364">Specialized heat-decrosslinking chemistry designed to reverse formalin-induced crosslinks and improve RNA recovery.</li>
<li data-section-id="1j7dh62" data-start="365" data-end="470">Spin-column purification technology that delivers clean RNA for reliable downstream molecular analysis.</li>
<li data-section-id="16nlq73" data-start="471" data-end="596">RNA extraction solutions optimized for archived tissue specimens used in biomarker, oncology, and gene expression research.</li>
<li data-section-id="17oavxo" data-start="597" data-end="710" data-is-last-node="">High-quality RNA preparation suitable for RT-PCR, qPCR, RNA sequencing, and other transcriptomics applications.</li>
</ul>
&nbsp;
<h2>How to Choose an FFPE RNA Extraction Kit</h2>
&nbsp;
Xylene-Free vs. Xylene-Based Deparaffinization
The Zymo Research Quick-RNA FFPE Miniprep Kit uses a proprietary Deparaffinization Solution that dissolves paraffin without xylene, eliminating fume-hood requirements for the deparaffinization step. QIAGEN RNeasy FFPE and AllPrep DNA/RNA FFPE Kits specify xylene or heptane as the primary deparaffinization solvent; both provide xylene-free alternatives in the protocol. Xylene-free workflows are preferred in high-throughput pathology labs where fume-hood access is a bottleneck.
RNA-Only vs. DNA/RNA Co-Extraction
For paired DNA sequencing and RNA expression profiling from the same archived specimen, the Zymo Quick-DNA/RNA FFPE Miniprep Kit (R1009) and QIAGEN AllPrep DNA/RNA FFPE Kit co-extract both analytes from the same tissue section, conserving limited archival material. For RNA-only applications (RT-qPCR, RNA-seq, NanoString), the Quick-RNA FFPE Miniprep Kit provides a simpler one-column workflow optimized for maximum RNA yield.
Section Thickness and Surface Area
Thinner sections (5-10 um) provide better proteinase K penetration and more complete crosslink reversal than thick sections (20 um). Use 2-4 sections per extraction as a starting point; tissue type and cellularity affect yield - highly cellular tumour sections yield more RNA per mg than fibrous stromal tissue. The Zymo Quick-RNA FFPE kit specifies a maximum of four sections &lt;=20 um with ~20 mm2 total surface area per extraction.
Amplicon Size for Downstream Assay
Before selecting a kit, confirm the expected RNA fragment size from your FFPE blocks. Blocks &lt;=5 years old typically yield RNA assayable at 150-200 bp amplicons; blocks &gt;10 years old may only reliably support amplicons of 60-100 bp. Select assays and library prep kits validated for fragmented FFPE RNA (e.g., Illumina TruSeq RNA Exome, Takara SMART-Seq FFPE).
DNase Treatment
Include on-column DNase I treatment for RT-qPCR and RNA-seq to remove gDNA contamination, which is particularly abundant in FFPE extracts because protein crosslinks trap gDNA in the same fraction as RNA. The Zymo Quick-RNA FFPE kit recommends optional on-column DNase I treatment; for sequencing applications, this step is mandatory.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
FFPE RNA quality expectations: RIN 1-4 for older blocks (&gt;5 years), 3-6 for recent blocks (&lt;3 years); A260/A280 &gt;= 1.8. RNA yield from 10 mg FFPE tissue is typically 100 ng to 2 ug depending on tissue type, block age, and fixation conditions. For RNA-seq from FFPE, short insert library preparation protocols tolerating fragmented input (fragment size 100-300 bp) are required; Illumina TruSeq RNA Exome and Takara SMART-Seq v4 FFPE are validated for FFPE RNA-seq at major cancer research centers. FFPE biobank samples from surgical pathology archives at institutions including MD Anderson Cancer Center and Vanderbilt University are used extensively in retrospective cancer transcriptomics studies, making reliable FFPE RNA extraction a clinical research priority. The Zymo Quick-RNA FFPE Miniprep is cited in published FFPE workflows for its xylene-free chemistry and compatibility with both standard RT-PCR and next-generation sequencing library preparation.
<section class="mbp-contact-cta"></section>
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.

FFPE

Quick-RNA™ FFPE MiniPrep (50 Preps.)

R1008 by Zymo Research is the Quick-RNA FFPE MiniPrep Kit (50 preps) for xylene-free total RNA extraction from FFPE, fresh, and frozen tissue. Up to 25 mg tissue input, ≥25 µL elution, ≤50 µg yield, A260/A280 and A260/A230 >1.8, with in-column DNase I treatment. Ready for RT-PCR and NGS.

<h2>What are Spin-Column Bacterial and Fungal RNA Extraction Kits?</h2>
&nbsp;
Spin-column bacterial and fungal RNA extraction kits isolate total RNA from Gram-positive bacteria, Gram-negative bacteria, yeasts, filamentous fungi, algae, and protozoa using ultra-high-density BashingBead mechanical cell wall disruption in a chaotropic lysis buffer, followed by RNA binding to a Zymo-Spin silica-membrane column, on-column washing, and elution in 6-100 ul of RNase-free water - all using a standard microcentrifuge. The complete protocol takes under 15 minutes for a batch of 1-12 samples, requiring only a vortex with a microcentrifuge tube adapter, a microcentrifuge, and standard pipetting equipment. No specialized robotics, vacuum manifolds, or magnetic separation racks are needed. Choose a spin-column bacterial/fungal RNA kit for manual extraction of 1-24 samples per run, when existing automation infrastructure does not support magnetic bead formats, or when the fastest possible turnaround from culture to RT-qPCR data is required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Pinpoint&trade; Slide RNA Isolation System for targeted extraction of total RNA directly from localized tissue sections on microscope slides
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-RNA&trade; Fungal/Bacterial MicroPrep Kit for highly concentrated, low-volume recovery of transcripts utilizing micro-elution spin-column matrices&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZR Fungal/Bacterial RNA MiniPrep for precise cell-wall shattering of yeast and recalcitrant spores&nbsp;
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
YeaStar&trade; RNA Kit efficiently permeabilizes tough yeast cell walls.&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Bacterial/Fungal RNA Kit</h2>
&nbsp;
Miniprep vs. Microprep Format
The Zymo Quick-RNA Fungal/Bacterial Miniprep processes up to 2x10^8 bacteria or 2x10^7 fungi per column, eluting in 25-100 ul - the standard choice for typical 1-5 ml bacterial cultures. The Microprep handles smaller inputs (up to 1x10^7 bacteria) and elutes in as little as 6 ul, concentrating RNA for sensitive applications such as single-cell-equivalent samples or very low-input transcriptomics.
Bead Size for Target Organism
Pre-mixed 0.1 mm + 0.5 mm BashingBead tubes in Zymo kits handle bacteria (0.1 mm) and fungi (0.5 mm) without selecting bead size. For pure Gram-negative bacterial cultures that do not require bead beating, direct lysis in buffer is available as a faster protocol option that skips the bead-beating step entirely.
On-Column DNase I Treatment
Include DNase I for all RT-qPCR and RNA-seq applications. Bacterial gDNA is abundant and co-purifies efficiently under silica-membrane binding conditions; without DNase treatment, gDNA contamination produces false amplification in RT-qPCR assays targeting genes with no introns. DNase I Reaction Mix is included in Zymo Quick-RNA Fungal/Bacterial kits.
Elution Volume
Elute in 25-50 ul for standard RT-qPCR (2-5 ul per reaction provides 5-25 RNA equivalents per assay). For RNA-seq library preparation requiring &gt;=200 ng input, use 50-100 ul elution volume to recover more total RNA mass; then measure concentration by Qubit and adjust for library prep.
Storage of Extracted RNA
Store spin-column bacterial RNA at -80C in single-use aliquots; mRNA from bacteria is AU-rich and degrades rapidly through multiple freeze-thaw cycles. Use within 6 months for transcriptomic applications; RT-qPCR targets with high expression levels remain quantifiable for 12+ months at -80C.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Target quality metrics for spin-column bacterial/fungal RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, and RIN &gt;= 7 from log-phase cultures processed within 60 seconds of harvest. Zymo Research Quick-RNA Fungal/Bacterial kits use the same Zymo-Spin column technology found across the Quick-RNA product line and are validated for inhibitor-free RNA suitable for NGS, RT-PCR, microarray, and hybridization. The Quick-RNA Fungal/Bacterial Miniprep is one of the most widely used spin-column formats for bacterial and fungal RNA in academic molecular microbiology research, with validated protocols for S. cerevisiae, C. albicans, A. fumigatus, and standard Gram-positive and Gram-negative species.&nbsp;
<section class="mbp-contact-cta"></section>
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for kit selection guidance, bulk pricing, and Zymo Research protocol adaptation for non-standard organisms.

Bacterial/Fungal RNA/ Spin-column

Quick-RNA™ Fungal/Bacterial MicroPrep Kit (50 Preps.)

Quick-RNA™ Fungal/Bacterial MicroPrep Kit R2010 by Zymo Research — 50 preps, RNA ≥17 nt, 10 µg binding, ≥6 µL elution, ≤10 min. ZR BashingBead Lysis Tubes for mechanical disruption of fungal (Candida, Aspergillus, Saccharomyces) and bacterial cell walls. A260/A280 &gt;1.8. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Applicable For

Elution Volume

Processing Time

Processing Volume

Type

Quick-RNA™ MidiPrep (25 Preps.)

Quick-RNA MidiPrep Kit (R1056) by Zymo Research — up to 1 mg RNA yield per prep, ≥17 nt, A260/A280 and A260/A230 &gt;1.8, ≥200 µl elution. 25 Preps, room temperature storage. Optimized for cells and soft tissue.

<h2>What are Spin-Column Plant RNA Extraction Kits?</h2>
&nbsp;
Spin-column plant RNA extraction kits purify total RNA from plant tissue - leaves, stems, roots, buds, flowers, fruit, and seeds - using a liquid nitrogen grinding or bead-beating disruption step followed by a dual-column silica-membrane workflow in a standard microcentrifuge. The Zymo Quick-RNA Plant Miniprep Kit uses a Zymo-Spin IIICG column to physically retain and discard genomic DNA while RNA passes through into the collection tube, then a Zymo-Spin IIC column to bind, wash, and elute total RNA including small RNAs &gt;=17 nt. The full protocol takes 20-30 minutes per batch of 1-12 samples and yields up to 50 ug RNA per extraction. Use the spin-column plant RNA format for routine lab use processing 1-24 plant tissue samples per day without automation infrastructure, or when your samples require a fast, phenol-free extraction workflow validated for standard crop and model plant species.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1ds2uf1" data-start="25" data-end="79">Recovery of ultra-pure total RNA from plant tissues.</li>
<li data-section-id="1n1wgzn" data-start="80" data-end="148">Effective processing of tough and difficult-to-lyse plant samples.</li>
<li data-section-id="197xy8o" data-start="149" data-end="210">Rapid RNA purification using silica spin-column technology.</li>
<li data-section-id="te47gz" data-start="211" data-end="257">Organic solvent-free RNA isolation workflow.</li>
<li data-section-id="1ru7scj" data-start="258" data-end="332" data-is-last-node="">High-quality RNA suitable for downstream molecular biology applications.</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Plant RNA Kit</h2>
&nbsp;
Tissue Type and Inhibitor Load
Soft, low-polyphenol tissues (Arabidopsis leaves, tobacco, rice leaves) extract cleanly with guanidinium-based spin-column kits. Fibrous, woody, or high-polyphenol tissues (mature stems, bark, fruit peel, seeds) require either CTAB-based upstream lysis followed by column purification, or additional grinding in liquid nitrogen to achieve complete disruption before adding lysis buffer.
CTAB Compatibility
Confirm the spin-column kit is compatible with CTAB-based pre-lysis if you work with polyphenol-rich species. Zymo Direct-zol RNA Miniprep Plus is confirmed compatible with CTAB-extracted samples from Pinus and Geranium; the lysis protocol for CTAB-compatible extraction is available from Zymo Research as a supplementary protocol.
Two-Column DNA Depletion vs. On-Column DNase
The two-column Zymo-Spin IIICG/IIC architecture provides physical gDNA depletion without a separate DNase incubation, saving 15 minutes per batch. For RNA-seq applications where any gDNA mapping artefacts must be eliminated, add optional on-column DNase I treatment after the IIICG column step. Plant genomes contain repetitive sequences that can map to cDNA targets; gDNA-free RNA is essential for accurate intron-spanning transcript quantification.
Inhibitor Removal Filter
Use Zymo-Spin III-HRC Spin Filters on the eluted RNA when A260/A230 &lt; 1.5, or when you know the tissue type is high in polysaccharides (starch-rich seeds, high-pectin fruit) or polyphenols. Each filter reduces the yield by 10-20% but substantially improves RT-qPCR amplification efficiency.
Elution Strategy
For RNA-seq: elute in 50-100 ul and quantify by Qubit RNA HS Assay for accurate low-concentration measurement. For RT-qPCR only: elute in 25-30 ul to concentrate RNA and reduce the volume needed per reaction well.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Quality benchmarks for spin-column plant RNA: A260/A280 &gt;= 1.8-2.0 (typical for plant RNA due to low protein content relative to total RNA), A260/A230 &gt;= 1.5 (target &gt;= 1.8 for RNA-seq), and RIN &gt;= 7 from well-processed fresh tissue. The Zymo Quick-RNA Plant Miniprep Kit achieves A260/A280 &gt; 1.8 and A260/A230 &gt; 1.8 for standard model plant species under its standard protocol. As of 2025, Arabidopsis thaliana and rice remain the most intensively studied model plant species; published plant RNA-seq methods from labs at institutions including the Howard Hughes Medical Institute and Vanderbilt University have validated spin-column plant RNA kits for transcriptome-wide expression profiling. For high-polyphenol crop species, including grapevine, citrus, and coffee, contact MBP's technical team for CTAB-compatible protocol guidance.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP Inc.</a> today and get high-quality spin-column systems for your advanced botanical research.

Quick-RNA™ Plant Miniprep Kit (50 Preps.)

The Quick-RNA™ Plant Miniprep Kit (R2024 by Zymo Research) purifies up to 50 µg of high-quality total RNA — including small RNAs ≥ 17 nt — from up to 150 mg of fresh, frozen, or lyophilized plant tissue. A260/A280 &gt; 1.8; ready for RT-qPCR, RNA-seq, and small RNA profiling.

<h2>What are Spin-Column Insect RNA Extraction Kits?</h2>
&nbsp;
Spin-column insect RNA extraction kits purify total RNA from whole arthropod specimens and insect tissue using ultra-high-density BashingBead mechanical homogenization in a chaotropic lysis buffer, followed by a two-column silica-membrane purification workflow in a standard microcentrifuge. The Zymo Quick-RNA Tissue/Insect Microprep Kit (R2030) uses a Zymo-Spin IIIC column to capture and discard genomic DNA in the flow-through, then a Zymo-Spin IC column to bind, wash, and elute total RNA, including small RNAs &gt;=17 nt. The full protocol completes in approximately 10 minutes for batches of 1-12 specimens. Choose the spin-column insect RNA format for manual extraction of single insects to small batches, for field work using DNA/RNA Shield stabilization, and for studies requiring co-isolation of miRNA and siRNA from the same arthropod specimen.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="bn9qob" data-start="0" data-end="116">Quick-RNA&trade; Tissue/Insect MicroPrep Kit for rapid isolation of ultra-pure total RNA from tissue and insect samples.</li>
<li data-section-id="1d6veh7" data-start="233" data-end="340">Ultra-clean spin-column technology designed to maximize RNA purity and recovery from small sample inputs.</li>
<li data-section-id="o7nhuv" data-start="341" data-end="463">RNA extraction solutions optimized for challenging biological specimens, including insect tissues and dissected samples.</li>
<li data-section-id="18gssqf" data-start="464" data-end="573">High-quality total RNA preparation suitable for RT-PCR, qPCR, RNA sequencing, and gene expression analysis.</li>
<li data-section-id="lpdewh" data-start="574" data-end="671" data-is-last-node="">Streamlined purification protocols that reduce processing time while maintaining RNA integrity</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Insect RNA Kit</h2>
&nbsp;
Microprep vs. Miniprep Format
The Microprep kit (Zymo R2030) processes specimens up to 10 mg and elutes in 6-25 ul, concentrating RNA for sensitive RT-qPCR from single small insects. The Miniprep format handles up to 30 mg of tissue - appropriate for dissected insect organs, larger arthropods, or small pools of 5-10 insects - and elutes in 25-100 ul for applications requiring higher total RNA mass.
Two-Column DNA Depletion vs. On-Column DNase
The two-column design (IIIC + IC column) in Zymo insect kits physically separates gDNA by binding capacity difference, eliminating a separate DNase incubation step. This is faster but less stringent than on-column DNase I treatment. For RNA-seq applications where any gDNA carryover introduces mapping artefacts, add an optional DNase I step; for RT-qPCR with intron-spanning primers, the two-column depletion alone is generally sufficient.
Bead Beating Instrument
A benchtop vortex (Vortex-Genie 2) with a horizontal microtube adapter provides validated lysis for the Zymo BashingBead Lysis Tubes at maximum speed for 5 minutes. A dedicated bead mill (e.g., Precellys, MP FastPrep) achieves more consistent disruption for hard-bodied arthropods such as hard ticks and beetles and is preferred when processing more than 24 specimens per session.
Specimen Stabilization Compatibility
Specimens in Zymo DNA/RNA Shield load directly into lysis tubes without additional washes or centrifugation steps. If specimens were field-collected in dry ice or liquid nitrogen, thaw at room temperature in lysis buffer and add to BashingBead tubes immediately - do not allow thawed specimens to sit at room temperature without lysis buffer, as RNase activity resumes rapidly on thawing.
Vector Surveillance Pooling
For arbovirus surveillance, pool up to 50 mosquitoes in the same lysis tube, cap tightly, and bead-beat at maximum speed for 10 minutes. Transfer 200-400 ul of clarified lysate (post-centrifugation) per column; multiple column reloads can process the remaining volume. Validate pool sensitivity with a spiked positive control before deploying in routine surveillance.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for spin-column insect RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.8, and RNA yield up to 10 ug from a single Aedes aegypti adult female. The Zymo Quick-RNA Tissue/Insect Microprep Kit is validated for RT-PCR, hybridization, microarray, and Next-Gen sequencing applications, and its two-column architecture is designed to deliver RNA free of PCR inhibitors - a critical requirement for arbovirus RT-qPCR assays where false-negative results from inhibitor carryover have direct public health implications. This kit is the primary insect RNA extraction format used in Drosophila immunity and mosquito vector competence research, with published protocols referencing BashingBead lysis in studies from institutions including Howard Hughes Medical Institute and Vanderbilt University.
<section class="mbp-contact-cta">
<h2>&nbsp;</h2>
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Quick-RNA™ Tissue/Insect MicroPrep Kit (50 Preps.)

Quick-RNA™ Tissue/Insect MicroPrep Kit R2030 by Zymo Research is a 50-prep kit for total RNA isolation (≥17 nt, including miRNAs) from insects, arthropods (mosquitoes, ticks, Drosophila), and tough-to-lyse tissue in 1–20 mg amounts. Includes BashingBead lysis tubes, Zymo-Spin IC and IIICG columns, minimum elution 6 µl, binding capacity 10 µg, purity A260/A280 >1.8. All components stored at room temperature.

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
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Zymo Buffers

RAD Buffer (RNA Agarose Dissolving Buffer) (50 ml)

R1011-1-50 by Zymo Research is a 50 mL RAD Buffer (RNA Agarose Dissolving Buffer) that simultaneously dissolves agarose gel slices and binds RNA for use with the Zymoclean Gel RNA Recovery Kit, enabling purification of RNA fragments ≥200 nt from TAE, TBE, MOPS, and formaldehyde-denaturing agarose gels.