Zymo Research

<h2>What are Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Spin-column environmental/microbial DNA/RNA co-extraction kits simultaneously purify genomic DNA and total RNA - including small RNAs 17-200 nt - from feces, soil, water, biofilm, swabs, plant material, and body fluids using bead-beating mechanical disruption with BashingBeads (0.1 mm + 0.5 mm) for unbiased lysis of all microbial cell types, followed by sequential Zymo-Spin column purification in a standard microcentrifuge. The ZymoBIOMICS DNA/RNA Miniprep Kit delivers DNA and RNA in separate fractions - the primary advantage over the combined total nucleic acid eluate of the MagBead format - enabling parallel metagenomic and metatranscriptomic library preparation from one undivided environmental input without post-extraction enzymatic separation steps. Choose the spin-column format when processing 1-24 environmental samples per run, when separate DNA and RNA fractions are required from the outset, or when automation infrastructure is unavailable.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA&trade; Water Kit to isolate high-purity nucleic acids from water samples using specialized column purification
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA&trade; Pathogen MiniPrep Kit for rapid co-extraction of pathogen genomes utilizing robust spin-column technology
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZymoBIOMICS&trade; DNA/RNA Mini Kit to ensure unbiased microbiome profile recoveries using an innovative lysis system&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Separate vs. Combined Fractions
The ZymoBIOMICS DNA/RNA Miniprep natively delivers separate DNA and RNA by column switching: gDNA binds the IIIC column while RNA flows through to the IC column. No post-extraction enzymatic treatment is needed to separate the analytes. This is the critical differentiator from the MagBead format, which requires DNase I treatment of the combined eluate to prepare an RNA fraction for RNA-seq.
PCR Inhibitor Removal Validation
Always measure A260/A230 on both eluates after extraction. Values below 1.5 indicate inhibitor carryover from humic acids (soil) or bile salts (feces) that will suppress PCR amplification and NGS library preparation. If A260/A230 &lt; 1.5, reduce input mass to 50-100 mg and add an optional pass through Zymo-Spin III-HRC Inhibitor Removal Columns on the RNA eluate before downstream use.
Bead-Beating Instrument
A vortex with a horizontal adapter (Vortex-Genie 2 at max speed x 5 min) validates for standard fecal and soil samples. A dedicated bead mill (Precellys, FastPrep-24) at 6,000-6,500 rpm for 40-60 seconds per cycle provides more consistent lysis for tough-walled Gram-positive organisms in high-diversity environmental samples; use 2-3 cycles with 30-second cooling intervals on ice to prevent RNA degradation from heat.
DNA/RNA Shield for Sample Stabilization
DNA/RNA Shield preserves microbial community RNA at room temperature for 30 days. Add Shield directly to the sample tube immediately after collection - before any transport or refrigeration delay - to capture the metabolic RNA expression state at the moment of collection. Shield-stabilized samples load directly into BashingBead Lysis Tubes without additional preparation.
Input Scaling for Low-Biomass vs. High-Biomass Samples
Standard input for feces: 250 mg. Standard input for topsoil: 200 mg. For low-biomass environmental samples (filtered water, air, oligotrophic deep soil): collect maximum biomass by filtering the largest feasible volume and add carrier RNA (5 ng per extraction) to the lysis step to improve column binding of trace nucleic acids. For high-biomass samples (feces, compost), reduce input to 50-100 mg to avoid column overloading and inhibitor carryover.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for the ZymoBIOMICS DNA/RNA Miniprep Kit: DNA yield 2-15 ug from 250 mg feces (A260/A280 &gt;= 1.8, fragment size &gt;= 15 kb for standard input); RNA yield 2-10 ug from 250 mg feces (A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, small RNAs 17-200 nt included). The kit uses Zymo-Spin column technology with OneStep PCR Inhibitor Removal Technology validated for feces, soil, plant material, water, biofilm, swabs, saliva, and body fluids, producing inhibitor-free nucleic acids ready for RT-PCR, arrays, sequencing, and microbiome analyses. The ZymoBIOMICS DNA/RNA Miniprep is widely used in gut microbiome and soil metatranscriptomics research at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
&nbsp;
For bulk pricing on ZymoBIOMICS DNA/RNA Miniprep kits, bead-beating instrument recommendations, or protocol guidance for low-biomass or high-inhibitor environmental samples,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.

Spin-column

MSDS

Protocol

Quick-DNA/RNA™ Water Kit (50 Preps)

R2044 by Zymo Research is a 50-prep kit for simultaneous DNA and RNA recovery from water, wastewater, and sludge samples. It processes up to 1 L (low-biomass water) or 50 mL (raw wastewater), yielding up to 25 µg DNA and 100 µg RNA per prep at A260/A280 >1.8 without vacuum filtration. Industry scientists supporting wastewater surveillance, environmental virology, or drinking water monitoring benefit from the inhibitor-removal technology and ddPCR/RT-qPCR/NGS-ready eluates.

Sample Sources

Sample Size

DNA/RNA Purity

Yield

DNA/RNA Storage

Equipment Needed (user provided)

Manufacturer

Shipping Condition

Storage Condition

<h2>What are microbial and environmental DNA isolation kits?</h2>
&nbsp;
Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
&nbsp;
<h2>How to choose a microbial and environmental DNA isolation kit</h2>
&nbsp;
Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

DataSheet

Quick-DNA™ Fecal/Soil Microbe 96 Kit (2 x 96 Preps.)

The Quick-DNA™ Fecal/Soil Microbe 96 Kit (D6011) by Zymo Research extracts inhibitor-free genomic DNA in a 96-well format from fecal samples (≤80 mg), soil (≤135 mg), and microbial cells. Silicon-A-HRC technology removes humic acids, polyphenolics, and melanins. Yields up to 5 µg DNA per prep at A260/A280 ≥1.8; 2 × 96 preps per kit; compatible with qPCR, NGS, and microarrays.

Applicable For

Elution Volume

Equipment

Processing Volume

Purity

Sample Source

Sample Storage

Size Range

Type

MSDS - D6010-FM

MSDS - D6011-FM

MSDS - D6012-FM

Optimized Lysis Protocols

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit

Zymo Research Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit: 96-well magnetic bead DNA extraction from ≤80 mg feces or ≤135 mg soil, ~90 min, ≤25 µg, ≥37.5 µl. Three variants: with Lysis Rack (D6010-FM), no matrix (D6011-FM), with Lysis Tubes (D6012-FM). All 2×96=192 preps.

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit (2 x 96 Preps) (includes ZR BashingBead™ Lysis Rack)

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit (2 x 96 Preps.)(Lysis Matrix Not Included)

Quick-DNA™ Fecal/Soil Microbe 96 Magbead Kit (2 x 96 Preps) (includes ZR BashingBead™ Lysis Tubes)

Quick-DNA™ Fecal/Soil Microbe DNA MiniPrep Kit (50 Preps.)

D6010 by Zymo Research is the Quick-DNA™ Fecal/Soil Microbe DNA MiniPrep Kit (50 preps). Isolates inhibitor-free DNA from feces (≤150 mg), soil (≤250 mg), or fungal/bacterial cells (≤100 mg). Yield ≤25 µg; purity A260/A280 ≥1.8; fragments up to 40 kb+. Uses ZR BashingBead™ Lysis Tubes. Processing time ~15 min after bead beating.

Quick-DNA™ Fecal/Soil Microbe MidiPrep (25 Preps.)

D6110 Quick-DNA Fecal/Soil Microbe MidiPrep Kit by Zymo Research — 25 preps, ≤375 mg feces or ≤5 g soil input, up to 125 µg DNA yield, ≥150 µl elution, ≤25 min, inhibitor-free.

<h2>What are FFPE DNA extraction kits?</h2>
&nbsp;
An FFPE DNA extraction kit purifies genomic DNA from formalin-fixed, paraffin-embedded tissue sections through a deparaffinization step (xylene-free in most current kits), proteinase K digestion to lyse fixed tissue, heat treatment to reverse formalin-induced crosslinks, and silica-membrane spin column purification, with some kits offering an optional uracil-N-glycosylase (UNG) treatment to remove deaminated-cytosine artifacts before NGS. Choose an FFPE-specific kit rather than a standard tissue kit whenever your sample is formalin-fixed and paraffin-embedded, since standard kits don't include deparaffinization or crosslink-reversal steps.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">Quick-DNA&trade; FFPE MiniPrep for rapid isolation of high-purity genomic DNA from FFPE tissue samples for high-throughput downstream applications.</li>
<li data-section-id="1n7706u" data-start="258" data-end="375">Designed to recover highly fragmented and cross-linked DNA suitable for sensitive downstream molecular workflows.</li>
<li data-section-id="355glt" data-start="376" data-end="494">Streamlined workflow supports rapid processing of FFPE samples with minimal hands-on time and reduced sample loss.</li>
<li data-section-id="125uabh" data-start="495" data-end="611">Optimized for compatibility with high-throughput applications, including PCR, sequencing, and genotyping assays.</li>
<li data-section-id="10hv3na" data-start="612" data-end="731" data-is-last-node="">Ensures consistent DNA yield and purity from challenging archived tissue specimens for reliable experimental results.</li>
</ul>
&nbsp;
<h2>How to choose an FFPE DNA extraction kit</h2>
&nbsp;
Deparaffinization chemistry
Most current FFPE DNA kits use a proprietary, xylene-free Deparaffinization Solution that dissolves paraffin without multiple wash steps, though some published methods use mineral oil as a simple alternative deparaffinization approach. Xylene-free chemistry reduces hands-on time and avoids handling a hazardous solvent, which matters for labs without dedicated solvent fume hoods.
Crosslink reversal and artifact removal
Formalin fixation creates DNA-protein crosslinks that block PCR unless reversed, so FFPE kits include a heat-based de-crosslinking step after lysis; some kits add an optional UNG treatment before a second lysis step to remove deaminated-cytosine artifacts, which is specifically useful for reducing C-to-T and G-to-A transition artifacts in NGS data. If your downstream application is NGS or dPCR on long-archived samples, a kit with UNG treatment is worth the extra step.
Sample input and elution volume
FFPE kits typically recommend 6-8 tissue sections of 5-10 um thickness as input, with some kits offering elution volumes as low as 15-30 ul for concentrated DNA suitable for limited-input downstream assays. If your archived sections are scarce, prioritize kits that minimize sample loss during deparaffinization rather than requiring multiple wash steps.
Expected DNA quality and fragment size
DNA from FFPE samples is inherently fragmented due to formalin fixation, and downstream applications involving fragments under 200 bp are generally achievable with effective deparaffinization and extraction; PCR success rates around 97% across microsatellite markers have been reported with optimized protocols on long-archived specimens. Set expectations for fragment size and PCR success rate based on sample age and fixation duration rather than assuming fresh-tissue-equivalent quality.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for FFPE DNA extraction are deparaffinization chemistry (xylene-free vs. solvent-based), whether crosslink reversal and optional UNG artifact removal are included, and elution volume relative to your sample's scarcity. A kit recommending 6-8 sections per extraction with elution as low as 15-30 ul concentrates limited archived material for downstream PCR or NGS. Labs working with both fresh and archived samples often keep&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/blood/tissue">blood/tissue genomic DNA kits</a>&nbsp;for fresh/frozen tissue alongside a dedicated FFPE kit for archived specimens, since standard tissue kits lack deparaffinization chemistry. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;and broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits">DNA extraction kits hub</a>&nbsp;link to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/urine">urine DNA extraction kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;for other specialized sample types.&nbsp;
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

FFPE

Quick-DNA™ FFPE MiniPrep

Zymo Research Quick-DNA™ FFPE MiniPrep: 50-prep kit, up to 25 µg DNA from FFPE tissue in ≥50 µl, A260/A280 ≥1.8, &gt;50 bp or &gt;500 bp fragment selection. Complete kit (D3067) + Deparaffinization Solution (D3067-1-20), Genomic DNA Wash 1 (D3067-2-25), Wash 2 (D3067-3-12).

Quick-DNA™ FFPE MiniPrep (50 Preps.)

Deparaffinization Solution (20 ml)

Genomic DNA Wash 1 (25 ml)

Genomic DNA Wash 2 (12 ml)

Quick-DNA™ Fungal/Bacterial 96 Kit (2 x 96 Preps.)

D6006 Quick-DNA Fungal/Bacterial 96 Kit by Zymo Research — 2×96 preps, BashingBead lysis, ≤40 min, ≤5 µg yield per well, 25–35 kb fragments, A260/A280 ≥1.8, qPCR and NGS ready.

Processing Time

Quick-DNA™ Fungal/Bacterial Microprep Kit (50 Preps.)

Quick-DNA™ Fungal/Bacterial Microprep Kit (D6007) by Zymo Research — 50 preps, ≤15 min. BashingBead mechanical lysis for fungi, Gram+/- bacteria, algae, and protozoa. Yields ≤5 µg DNA at 25–35 kb fragments; elution ≥10 µl; A260/A280 ≥1.8.

Quick-DNA™ Fungal/Bacterial Midiprep Kit (25 Preps.)

D6105 by Zymo Research isolates up to 125 µg of genomic DNA from 250–500 mg of tough-to-lyse fungi or bacteria in as little as 20 minutes using mechanical bead-beating lysis. The Quick-DNA™ Fungal/Bacterial Midiprep Kit (25 preps) produces high-molecular-weight DNA (25–35 kb, A260/A280 &gt;1.8) suitable for PCR, WGS, RFLP, and Southern blotting. All reagents store at room temperature.

Quick-DNA™ Fungal/Bacterial Miniprep Kit (50 Preps.)

The Quick-DNA™ Fungal/Bacterial Miniprep Kit (D6005) by Zymo Research extracts up to 25 µg of total genomic DNA from fungi, bacteria, spores, algae, and protozoa in ≤15 minutes using BashingBead mechanical lysis. Typical DNA fragment size 25–35 kb; purity A260/A280 ≥1.8. 50 preps per kit; eluate is compatible with qPCR, NGS, and arrays.

<h2>What Are Magnetic Bead HMW DNA Extraction Kits?</h2>
&nbsp;
A magnetic bead HMW DNA extraction kit uses Solid Phase Reversible Immobilization (SPRI)-type magnetic beads to bind genomic DNA released by gentle lysis, with a magnetic separator handling wash and elution steps in place of centrifugation, columns, or vacuum manifolds, producing fragment sizes in the 50-150 kb range depending on how beads are resuspended during elution. Related entities include PacBio Nanobind-type bead chemistry, KingFisher-compatible automated magnetic particle processors, A260/280 and A260/230 purity thresholds above 1.8, and downstream long-read sequencing library preparation for PacBio and Oxford Nanopore platforms. Choose magnetic bead HMW kits when fragment sizes in the 50-150 kb range meet your long-read sequencing needs, and you want to avoid centrifugation-based shearing.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Low-shear high molecular weight DNA isolation solutions using advanced paramagnetic bead technology, including the Magbio HighPrep HMW DNA Kit.</li>
<li>High-throughput magnetic bead-based extraction systems for simultaneous recovery of intact genomic DNA, including the Quick-DNA&trade; HMW Kit.</li>
<li>Workflow-friendly HMW DNA purification kits designed to preserve long DNA fragments for long-read sequencing applications.</li>
<li>Solutions compatible with manual and automated sample processing for efficient genomic DNA recovery.</li>
<li>HMW DNA extraction products suitable for genome assembly, structural variant analysis, and other advanced genomics workflows.</li>
</ul>
&nbsp;
<h2>How to Choose a Magnetic Bead HMW DNA Kit</h2>
&nbsp;
Bead Resuspension Method and Fragment Size Tradeoff
Fragment size from magnetic bead HMW kits depends on how beads are resuspended after washing: gentle tube tapping typically yields fragments in the 50-150 kb range, while vortexing produces somewhat smaller fragments (around 40-100 kb) but may improve recovery consistency. If your sequencing application benefits from the largest possible fragments (e.g., resolving large structural variants), prioritize tapping-based resuspension protocols even if yield is marginally lower.
Sample Input and Expected Yield
Magnetic bead HMW kits for blood, cells, and tissue typically specify inputs such as 200 ul of fresh/frozen whole blood, 1 million cells, or up to 8 mg of tissue, with a minimum yield around 3 ug of HMW gDNA&mdash;matching the input requirements of PacBio library preparation (around 1 ug above 30 kb) and Oxford Nanopore ligation kits (100 ng to 1.5 ug). Confirm your sample type matches a documented protocol within the kit family rather than assuming a single universal protocol.
No Centrifuge, Column, or Vacuum Required
Unlike silica spin-column or 96-well plate HMW approaches, magnetic bead HMW kits eliminate centrifugation, columns, and vacuum manifolds entirely, relying only on a magnetic separator for all wash and elution steps. This simplifies equipment requirements for labs without access to specialized centrifuge rotors, though a magnetic stand or automated magnetic particle processor is still required.
Purity Ratios and RNA Removal
A260/280 and A260/230 ratios greater than 1.8 are generally preferred for HMW DNA from magnetic bead kits, with near-complete RNA removal reported as a feature of bead-based HMW chemistry. Ratios below this threshold can indicate protein or salt contamination requiring troubleshooting before proceeding to expensive long-read library preparation.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
The defining specifications for magnetic bead HMW DNA extraction are the fragment size achievable via the bead resuspension method chosen, sample-specific input requirements relative to the kit's documented minimum yield, and purity ratios above 1.8 for both A260/280 and A260/230. A kit yielding around 3 ug of DNA in the 50-150 kb range from 200 ul whole blood, 1 million cells, or up to 8 mg tissue meets standard PacBio and Nanopore library preparation inputs without centrifugation.&nbsp;
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Mag Beads

Quick-DNA™ HMW kit (96 Preps.)

The D6060 by Zymo Research is a 96-prep magnetic bead-based HMW DNA extraction kit recovering fragments up to 150 kb (typical 20–200 kb) at A260/A230 ≥ 1.8, with 10 µg binding capacity per 50 µl beads. Compatible with cells, tissue, blood, saliva, and buccal swabs; optimized for Oxford Nanopore and PacBio SMRT long-read sequencing. Made in the USA.

DNA Purity

DNA Size

DNA Yield

Sample Types

<h2>What Are Magnetic Bead Genomic DNA Kits for Blood and Tissue?</h2>
&nbsp;
Magnetic bead genomic DNA kits for blood and tissue use paramagnetic beads to capture genomic DNA released during sample lysis. Following DNA binding, a magnetic separator immobilizes the beads, and contaminants are removed by wash steps before purified DNA is eluted. Unlike spin-column workflows, magnetic bead protocols eliminate centrifugation and simplify automation, making them well-suited to high-throughput laboratories and automated liquid-handling systems.
Magnetic bead extraction is often selected when automation, reduced hands-on processing, or compatibility with high-throughput workflows is a priority. Specialized high-molecular-weight (HMW) magnetic bead chemistries are also available for applications requiring longer DNA fragments for advanced sequencing workflows.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li>Quick-DNA&trade; Magbead Plus Kit: Magnetic bead-based genomic DNA purification for blood, tissue, cultured cells, and other biological samples.</li>
<li>Automation-Compatible Workflows: Designed for seamless integration with manual and automated magnetic separation platforms.</li>
<li>High-Yield DNA Recovery: Optimized to produce purified genomic DNA suitable for PCR, qPCR, genotyping, sequencing, and other downstream molecular biology applications.</li>
<li>Streamlined Sample Processing: Eliminates centrifugation steps through magnetic bead technology, simplifying genomic DNA extraction workflows.</li>
</ul>
<h2>&nbsp;</h2>
<h2>How to Choose a Magnetic Bead Genomic DNA Kit for Blood and Tissue</h2>
&nbsp;
Sample Input and Processing Time
Most magnetic bead kits are designed to process approximately 100-400 &micro;L of whole blood or buffy coat per preparation. Tissue and cultured cell inputs vary by kit but generally support small tissue samples and several million cells per extraction. Manual workflows can often be completed in about 20 minutes because centrifugation steps are eliminated.
Yield and Purity Requirements
Magnetic bead extraction kits are capable of producing high-quality genomic DNA suitable for PCR, qPCR, genotyping, sequencing, Southern blotting, and related molecular biology applications. DNA purity is commonly comparable to silica-based purification methods, making these kits suitable for most downstream workflows.
Automation Compatibility
One of the primary advantages of magnetic bead chemistry is its compatibility with automated liquid handling platforms. Because DNA capture and washing occur through magnetic separation rather than centrifugation, these workflows can be readily adapted to 96-well and higher-throughput automated formats.
High-Molecular-Weight DNA Applications
Some magnetic bead systems are specifically optimized for high-molecular-weight genomic DNA recovery. These workflows can preserve larger DNA fragments required for long-read sequencing, linked-read assembly, structural variation analysis, and other applications where DNA integrity is critical.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Magnetic bead genomic DNA extraction kits are differentiated by their centrifuge-free workflow, automation compatibility, and flexibility across blood, buffy coat, tissue, and cultured cell samples. The magnetic separation process reduces manual handling steps while supporting consistent DNA recovery and scalable throughput.&nbsp;Many laboratories adopt magnetic bead workflows when transitioning from manual extraction methods to automated sample preparation systems. Compared with traditional spin-column methods, magnetic bead purification simplifies integration with robotic liquid handlers and high-throughput extraction platforms while maintaining DNA quality suitable for PCR, qPCR, sequencing, and genotyping applications.&nbsp;When selecting a kit, evaluate sample input requirements, automation compatibility, DNA purity specifications, and whether high-molecular-weight DNA recovery is required for downstream applications. Most kit reagents are stored according to manufacturer recommendations, and lot-specific Certificates of Analysis (CoAs) are available upon request.
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> to get high-quality mag beads for blood/tissue genomic DNA extraction workflows to scale up your laboratory throughput.

D4081-Data Sheet

D4081-1-MSDS

D4081-2-MSDS

D4081-3-MSDS

D4081-Protocol

Quick-DNA™ Magbead Plus Kit

Zymo Research Quick-DNA™ Magbead Plus Kit: 96-well magnetic bead total DNA extraction from fluids, cells, and solid tissues — 10 µg binding, ≤150 kb, ~60 min, automation ready. D4081 (96 preps), D4082 (4×96). Buffer components: Solid Tissue Digestion Buffer II (D4081-2-6/22) and BioFluid &amp; Solid Tissue Buffer (D4081-3-25/100).