Zymo Reagents

<h2>What Are Zymo Research Buffers?</h2>
Zymo Research buffers are formulation-specific reagents used at defined steps &mdash; lysis, binding, wash, and elution &mdash; in Zymo-Spin column-based purification protocols for DNA, RNA, and bisulfite-converted DNA. Each buffer is optimized for a specific kit family: DNA Wash Buffer concentrate (requires ethanol addition before use) removes salts and contaminants before elution from Zymo-Spin silica columns; DNA Elution Buffer (10 mM Tris, pH 8.5, 0.1 mM EDTA) releases purified DNA from the matrix; RNA Wash Buffer supports RNA extraction kits including Quick-RNA and Direct-zol lines; M-Elution Buffer is designed for eluting bisulfite-converted DNA in EZ DNA Methylation kits. Researchers typically order these separately when a single buffer component runs out before the rest of the kit, or when scaling up a protocol beyond the volume included.
TL;DR: Order Zymo buffers individually to replenish a specific step in your existing Zymo workflow without replacing the full kit.
<h2>How to Choose Zymo Research Buffers</h2>
<h3>Match the buffer to your kit family</h3>
Every Zymo buffer is paired to a specific kit line. DNA Wash Buffer and DNA/RNA Wash Buffer concentrate are used in DNA Clean &amp; Concentrator, Quick-DNA, and Zymoclean Gel DNA Recovery kits. RNA Wash Buffer pairs with Quick-RNA kits. ZymoBIOMICS DNA Wash Buffer 1 and Wash Buffer 2 are specific to the ZymoBIOMICS microbiomics line. Substituting a buffer from a different kit family can compromise yield or purity.
<h3>Confirm ethanol addition requirements before use</h3>
Several Zymo wash buffer concentrates require addition of 96-100% ethanol to the bottle before first use &mdash; the label states the exact volume. DNA Wash Buffer concentrate, DNA/RNA Wash Buffer concentrate, and Urine DNA Wash Buffer concentrate all require this step. Pre-made (non-concentrate) versions such as RNA Wash Buffer are supplied ready to use.
<h3>Select the right volume for your throughput</h3>
Zymo buffers are available in sizes from 6 ml (single-experiment replenishment) up to 500 ml (Buffer P1 Red, for high-volume plasmid prep labs). For labs processing more than 50 samples per week, ordering the largest available size reduces per-sample cost and the frequency of reordering.
<h3>Choose elution buffer chemistry based on downstream application</h3>
DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA) is the standard for most PCR and sequencing applications. For applications sensitive to EDTA &mdash; such as some restriction enzyme digests or direct use with divalent cation-dependent enzymes &mdash; eluting in molecular-grade water or a Tris-only buffer is preferable. M-Elution Buffer is specifically formulated for bisulfite-converted DNA and should not be substituted with standard elution buffer in methylation workflows.
<h3>Verify research-use-only status</h3>
All Zymo Research buffers sold through MBP are for research use only (RUO). They are not cleared for in vitro diagnostic use. For clinical or diagnostic laboratory applications, confirm regulatory status with your institutional compliance officer before use.
<h2>Compare Zymo Buffer Types</h2>
<table>
<thead>
<tr>
<th>Buffer Type</th>
<th>Primary Application</th>
<th>Kit Family</th>
<th>Format Available</th>
</tr>
</thead>
<tbody>
<tr>
<td>DNA Wash Buffer (concentrate)</td>
<td>Salt removal before DNA elution</td>
<td>DNA Clean &amp; Concentrator, Quick-DNA</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA/RNA Wash Buffer (concentrate)</td>
<td>Salt removal for dual DNA/RNA preps</td>
<td>Quick-DNA/RNA kits</td>
<td>6 ml &ndash; 200 ml</td>
</tr>
<tr>
<td>DNA Elution Buffer (10 mM Tris pH 8.5, 0.1 mM EDTA)</td>
<td>DNA release from silica column</td>
<td>All Zymo DNA purification</td>
<td>16 ml &ndash; available sizes vary</td>
</tr>
<tr>
<td>RNA Wash Buffer</td>
<td>RNA purification wash step</td>
<td>Quick-RNA kits</td>
<td>Ready-to-use</td>
</tr>
<tr>
<td>M-Elution Buffer</td>
<td>Elution of bisulfite-converted DNA</td>
<td>EZ DNA Methylation kit families</td>
<td>Small volume</td>
</tr>
<tr>
<td>ZymoBIOMICS DNA Wash Buffer 2</td>
<td>Metagenomic/microbiomic DNA cleanup</td>
<td>ZymoBIOMICS DNA kits</td>
<td>Sold separately</td>
</tr>
<tr>
<td>Buffer P1 Red</td>
<td>Alkaline lysis resuspension (plasmid prep)</td>
<td>Zyppy Plasmid Miniprep</td>
<td>10 ml &ndash; 500 ml</td>
</tr>
</tbody>
</table>
<h2>Specifications Context</h2>
Zymo wash buffer concentrates are supplied at a defined fold-concentration (commonly 4x or 6x) and require ethanol addition to reach working strength &mdash; the exact ratio is printed on the bottle and in the kit protocol. Elution buffer volumes for Zymo-Spin columns typically range from 6 to 100 &micro;l per elution; larger elution volumes improve total yield at the cost of DNA concentration. For DNA elution from Zymo-Spin columns, the protocol recommends a minimum 1-minute contact time between elution buffer and the silica matrix to maximize recovery of fragments 10 kb and above. As of 2025, Zymo Research tests all buffer lots for performance consistency, with integrity guaranteed for up to one year from purchase date.
Browse the full <a href="https://www.notion.so/reagents">Zymo Research reagents collection at MBP</a> for kits and columns that use these buffers, or visit our <a href="https://www.notion.so/reagents/buffers">reagent buffers category</a> for non-Zymo buffer options including ABM and other brands. For volume orders or purchase orders, use the <a href="https://www.notion.so/quickorder">Quick Order</a> tool or <a href="https://www.notion.so/contact-us">contact us</a> directly &mdash; MBP is a registered vendor for institutional procurement systems.
Last updated: June 2025
&nbsp;

Zymo Buffers

MSDS

ZymoBIOMICS MagBinding Beads

D4302-6 by Zymo Research is the ZymoBIOMICS™ MagBinding Beads—a magnetic bead suspension for use in ZymoBIOMICS magnetic bead-based DNA purification kits for high-throughput microbiome and metagenomic DNA extraction from fecal, soil, water, and biofilm samples in 96-well automation formats. Available as 6 mL or 12 mL standalone bottles for bead replenishment.

Manufacturer

Shipping Condition

Storage Condition

ZymoBIOMICS™ MagBinding Beads (12 ml)

ZymoBIOMICS™ MagBinding Beads (6 ml)

<h2>What are microbiome standards and controls?</h2>
&nbsp;
Microbiome standards and controls are defined reference materials, ranging from a single microbial DNA standard to a complex mock community containing multiple known bacterial, fungal, or archaeal species at defined relative abundances, used to validate that an extraction, library preparation, and sequencing pipeline is performing as expected. Running a standard alongside real samples provides a specific, customized control for confirming reproducibility and enables meaningful comparison of results generated in different laboratories or at different times.
&nbsp;
<h2 dir="ltr">What you will discover:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
ZymoBIOMICS&trade; Community Standards: Mock communities tested for reproducibility with specific microbial ratios to assess taxonomic profiling and ensure accuracy across sequencing platforms.
</li>
<li dir="ltr" aria-level="1">
Log-Distributed DNA Standards: Customized genomic blends created to assess the limit of detection (LOD) and linear dynamic range for identifying rare taxa.
</li>
<li dir="ltr" aria-level="1">
Microbial Spike-in Controls: Internal standards with high and low loads for precise cell quantification and normalization of data across various matrices in simultaneous processing.
</li>
<li dir="ltr" aria-level="1">
Site-Targeted Reference Materials: Specialized standards for gut, oral, and fecal analysis, employing TruMatrix&trade; technology to replicate authentic spatial transcriptomics environments.
</li>
<li dir="ltr" aria-level="1">
High Molecular Weight (HMW) DNA: Ultra-pure, elongated templates designed explicitly to standardize long-read sequencing systems and verify assembly efficacy.
</li>
</ul>
&nbsp;
<h2>How to choose microbiome standards and controls</h2>
&nbsp;
Match standard complexity to your validation goal
A single-species microbial DNA or inactivated bacteria standard is sufficient for confirming basic assay sensitivity or specificity, while a multi-species mock community standard with defined relative abundances is needed to evaluate extraction and amplification bias across an entire workflow.
Use a standard to benchmark a new extraction or library prep protocol
Running a known mock community through a new or modified extraction and library preparation protocol, then comparing the resulting sequencing output against the standard's defined composition, reveals whether the new protocol introduces meaningful bias compared with an established method.
Confirm whether your standard supports both DNA-based and RNA-based workflows
Some standards are formulated specifically for DNA-based 16S or shotgun metagenomic workflows, while others, or specific lots, are validated for RNA extraction and metatranscriptomic workflows as well, a distinction worth checking against your specific application.
Include a standard when comparing results across different laboratories
Because microbiome sequencing results can be sensitive to differences in extraction method, primer choice, and sequencing platform between labs, including the same reference standard across collaborating sites supports more reliable comparison of results generated independently.
Confirm standard storage and stability before long-term use
Microbial community standards typically have documented stability windows and storage temperature requirements, and confirming these align with your intended use timeline helps avoid degraded standard material producing misleading validation results.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Individual microbial DNA and inactivated bacteria standards add value specifically by providing a specific, customized control, supporting both microbiomics and metagenomics workflows by increasing reproducibility and allowing reliable comparison of results from different laboratories. As of 2026, defined mock community standards remain a standard tool for benchmarking new extraction or library preparation protocols against established methods before adopting a new workflow for real sample processing.
<a href="https://mbpinc.net/contact-us">Reach out to the expert team at MBP Inc.</a> now to obtain a quote for the appropriate microbial standards for your lab.

Microbial Standards & Controls

DataSheet

Protocol

ZymoBIOMICS Microbial Community DNA Standard

D6305 by Zymo Research is a microbial community DNA standard composed of genomic DNA from eight bacterial and two fungal strains at defined log-distribution proportions, with DNA fragments less than 15 kb, designed to assess bias and performance in microbiomics and metagenomics sequencing workflows.

Applicable For

Purity

Sample Source

Processing Volume

Yield

Sample Storage

ZymoBIOMICS™ Microbial Community DNA Standard (200 ng)

ZymoBIOMICS™ Microbial Community DNA Standard (2000 ng)

ZymoBIOMICS Microbial Community DNA Standard II (Log Distribution)

ZymoBIOMICS Microbial Community DNA Standard II Log Distribution (D6311) by Zymo Research — 220 ng/20 µl, 10 microbial strains (8 bacteria + 2 yeasts) at logarithmic abundances spanning 6 orders of magnitude. &lt;0.01% foreign DNA; detects as few as 3 DNA copies. For NGS metagenomics QC and workflow bias detection. Made in the USA.

ZymoBIOMICS Spike-in Control I (High Microbial Load)

The D6320 by Zymo Research is a ZymoBIOMICS Spike-in Control I for high microbial load samples, containing equal cell numbers of fully inactivated Imtechella halotolerans and Allobacillus halotolerans (&lt;0.01% foreign DNA impurity) stored in DNA/RNA Shield at −80°C. Added before extraction, D6320 enables absolute microbial quantification and extraction QC in 16S rRNA amplicon sequencing and metagenomic workflows.

Biosafety

Impurity Level

Reference Genomes

Storage Solution

ZymoBIOMICS™ Spike-in Control I (High Microbial Load) (25 Preps.)

ZymoBIOMICS™ Spike-in Control I (High Microbial Load) (250 Preps.)

ZymoBIOMICS™ BashingBead Buffer (150 ml)

D4302-1-150 by Zymo Research is a 150 mL bottle of ZymoBIOMICS™ BashingBead Buffer — 750 µL used per sample with ZR BashingBead Lysis Tubes (0.1/0.5 mm) for mechanical bead-beating lysis of microbial cells in fecal, soil, water, and tissue samples. For use with ZymoBIOMICS DNA and DNA/RNA extraction kits.

<h2>What are microbial and environmental DNA isolation kits?</h2>
&nbsp;
Microbial and environmental DNA isolation kits purify DNA from soil, sediment, freshwater, marine water, feces, and other complex environmental matrices using lysis and purification chemistry designed to remove humic acids, metal compounds, and other inhibitors that interfere with PCR and sequencing, supporting 16S/18S rRNA amplicon profiling and shotgun metagenomic sequencing. Related entities include "kitome" and "splashome" contamination controls, alpha diversity and microbial community composition analysis, and compatibility with both short-read (Illumina) and long-read (Oxford Nanopore) sequencing platforms. Choose a kit validated for your specific sample matrix (soil, sediment, water, or feces), since benchmarking studies consistently show kit performance varies by matrix rather than one kit being universally best.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Spin-column fungal and bacterial kits for rapid DNA purification using rapid silica-membrane technology&nbsp;</li>
<li dir="ltr" role="presentation">Parallel extraction of soil, fecal, and environmental samples using high-throughput 96-well microplates&nbsp;</li>
<li dir="ltr" role="presentation">Automated&nbsp; purification of complex microbial and environmental specimens using Magnetic bead isolation systems</li>
<li dir="ltr" role="presentation">Scalable microprep and midiprep columns tailored for concentrated low-volume elution demands and diverse sample inputs</li>
</ul>
&nbsp;
<h2>How to choose a microbial and environmental DNA isolation kit</h2>
&nbsp;
Matrix-specific kit performance
Benchmarking studies comparing multiple commercial kits across sediment, water, soil, oyster gut microbiome, and mammalian feces have found that the best-performing kit varies by matrix - kits ranked highly for sediment did not necessarily perform best for water or host-associated samples. Rather than assuming a single "universal" kit, match your kit choice to published benchmarking results for your primary sample type where available.
Inhibitor removal for downstream PCR and sequencing
Environmental samples, especially sediments and soils, contain organic and metal compounds that inhibit PCR amplification if not removed during extraction; kits designed for these matrices integrate inhibitor-removal steps as part of the standard protocol. If your samples have historically produced low PCR amplification efficiency with general-purpose kits, an environmental-matrix-specific kit with built-in inhibitor removal is the first thing to evaluate.
Short-read vs. long-read sequencing compatibility
For long-read metagenomic sequencing, DNA fragment length and integrity from the extraction kit directly affect achievable read lengths, though longer extracted fragments don't always translate consistently into longer sequencing reads - kit choice affects both fragment length and downstream classification rate and reproducibility. If long-read metagenomics is your goal, prioritize kits with published long-read benchmarking data over kits validated only for short-read amplicon work.
Contamination control (kitome/splashome)
Kit-derived contamination ("kitome") and cross-sample contamination during processing ("splashome") can affect microbial community composition results, particularly for low-biomass samples; benchmarking studies evaluate kits specifically for these contamination sources alongside yield and diversity metrics. For low-biomass environmental samples, prioritize kits with documented low kitome contamination in published comparisons.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for microbial and environmental DNA isolation are matrix-specific validation (soil vs. sediment vs. water vs. feces), built-in inhibitor removal for PCR-ready output, and fragment length/integrity for long-read sequencing compatibility where relevant. Since benchmarking studies repeatedly show no single kit performs best across all matrices, identifying your primary sample type before selecting a kit is the most impactful decision. Labs running metagenomic surveys across multiple matrices (e.g., soil and water from the same field site) often need separate matrix-optimized kits rather than a single universal kit. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits hub</a>&nbsp;links to related sample-type categories, including&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/insect">insect genomic DNA kits</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna/plant">plant genomic DNA kits</a>&nbsp;for organism-associated environmental work, and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/high-molecular-weight-dna">high molecular weight DNA kits</a>&nbsp;for long-read metagenomics requiring larger fragment sizes. For cell-free DNA in environmental water samples (eDNA detection), see&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/cell-free-dna">cell-free DNA kits</a>.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Microbial & Environmental DNA Isolation

MSDS - D4301

Optimized Lysis Protocols

MSDS - D4305

ZymoBIOMICS™ DNA Microprep Kit

D4301 ZymoBIOMICS DNA Microprep Kit by Zymo Research — 50 preps; BashingBead lysis; 20 &micro;l elution; up to 5 &micro;g yield; 15&ndash;20 kb DNA; OneStep PCR inhibitor removal; feces, soil, swabs, cells.

Elution Volume

Equipment

Size Range

Type

ZymoBIOMICS™ DNA Microprep Kit (Lysis Tubes) (50 Preps.)

ZymoBIOMICS™ DNA Microprep Kit (No Lysis Matrix) (50 Preps.)

D4300/D4300T - MSDS

D4300-1 - MSDS

D4300-2 - MSDS

D4300-3 - MSDS

D4300-4 - MSDS

D4304 - MSDS

D4304-Protocol

D4300-Optimized Lysis Protocol

ZymoBIOMICS™ DNA Miniprep Kit

D4300 by Zymo Research: ZymoBIOMICS™ DNA Miniprep Kit, 50 preps. Extracts inhibitor-free microbial DNA from feces (≤200 mg), soil (≤250 mg), and cells (≤100 mg). Yield up to 25 µg; 15–20 kb DNA; BashingBead lysis; HRC inhibitor removal. Validated for microbiome and metagenomics. Made in USA.

ZymoBIOMICS™ DNA Miniprep Kit (50 Preps.)

ZymoBIOMICS™ DNA Miniprep Kit (5 Preps.)

ZymoBIOMICS™ Lysis Solution (150 ml)

ZymoBIOMICS™ Lysis Solution (40 ml)

ZymoBIOMICS™ DNA Binding Buffer (100 ml)

ZymoBIOMICS™ DNA Binding Buffer (250 ml)

ZymoBIOMICS™ DNA Wash Buffer 1 (100 ml)

ZymoBIOMICS™ DNA Wash Buffer 1 (50 ml)

ZymoBIOMICS™ DNA Wash Buffer 2 (200 ml)

ZymoBIOMICS™ DNA Wash Buffer 2 (60 ml)

ZymoBIOMICS™ HRC Prep Solution (30 ml)

<h2>What are Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kits?</h2>
&nbsp;
Spin-column environmental/microbial DNA/RNA co-extraction kits simultaneously purify genomic DNA and total RNA - including small RNAs 17-200 nt - from feces, soil, water, biofilm, swabs, plant material, and body fluids using bead-beating mechanical disruption with BashingBeads (0.1 mm + 0.5 mm) for unbiased lysis of all microbial cell types, followed by sequential Zymo-Spin column purification in a standard microcentrifuge. The ZymoBIOMICS DNA/RNA Miniprep Kit delivers DNA and RNA in separate fractions - the primary advantage over the combined total nucleic acid eluate of the MagBead format - enabling parallel metagenomic and metatranscriptomic library preparation from one undivided environmental input without post-extraction enzymatic separation steps. Choose the spin-column format when processing 1-24 environmental samples per run, when separate DNA and RNA fractions are required from the outset, or when automation infrastructure is unavailable.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA&trade; Water Kit to isolate high-purity nucleic acids from water samples using specialized column purification
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
Quick-DNA/RNA&trade; Pathogen MiniPrep Kit for rapid co-extraction of pathogen genomes utilizing robust spin-column technology
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
ZymoBIOMICS&trade; DNA/RNA Mini Kit to ensure unbiased microbiome profile recoveries using an innovative lysis system&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose a Spin-Column Environmental/Microbial DNA/RNA Co-Extraction Kit</h2>
&nbsp;
Separate vs. Combined Fractions
The ZymoBIOMICS DNA/RNA Miniprep natively delivers separate DNA and RNA by column switching: gDNA binds the IIIC column while RNA flows through to the IC column. No post-extraction enzymatic treatment is needed to separate the analytes. This is the critical differentiator from the MagBead format, which requires DNase I treatment of the combined eluate to prepare an RNA fraction for RNA-seq.
PCR Inhibitor Removal Validation
Always measure A260/A230 on both eluates after extraction. Values below 1.5 indicate inhibitor carryover from humic acids (soil) or bile salts (feces) that will suppress PCR amplification and NGS library preparation. If A260/A230 &lt; 1.5, reduce input mass to 50-100 mg and add an optional pass through Zymo-Spin III-HRC Inhibitor Removal Columns on the RNA eluate before downstream use.
Bead-Beating Instrument
A vortex with a horizontal adapter (Vortex-Genie 2 at max speed x 5 min) validates for standard fecal and soil samples. A dedicated bead mill (Precellys, FastPrep-24) at 6,000-6,500 rpm for 40-60 seconds per cycle provides more consistent lysis for tough-walled Gram-positive organisms in high-diversity environmental samples; use 2-3 cycles with 30-second cooling intervals on ice to prevent RNA degradation from heat.
DNA/RNA Shield for Sample Stabilization
DNA/RNA Shield preserves microbial community RNA at room temperature for 30 days. Add Shield directly to the sample tube immediately after collection - before any transport or refrigeration delay - to capture the metabolic RNA expression state at the moment of collection. Shield-stabilized samples load directly into BashingBead Lysis Tubes without additional preparation.
Input Scaling for Low-Biomass vs. High-Biomass Samples
Standard input for feces: 250 mg. Standard input for topsoil: 200 mg. For low-biomass environmental samples (filtered water, air, oligotrophic deep soil): collect maximum biomass by filtering the largest feasible volume and add carrier RNA (5 ng per extraction) to the lysis step to improve column binding of trace nucleic acids. For high-biomass samples (feces, compost), reduce input to 50-100 mg to avoid column overloading and inhibitor carryover.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Performance benchmarks for the ZymoBIOMICS DNA/RNA Miniprep Kit: DNA yield 2-15 ug from 250 mg feces (A260/A280 &gt;= 1.8, fragment size &gt;= 15 kb for standard input); RNA yield 2-10 ug from 250 mg feces (A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, small RNAs 17-200 nt included). The kit uses Zymo-Spin column technology with OneStep PCR Inhibitor Removal Technology validated for feces, soil, plant material, water, biofilm, swabs, saliva, and body fluids, producing inhibitor-free nucleic acids ready for RT-PCR, arrays, sequencing, and microbiome analyses. The ZymoBIOMICS DNA/RNA Miniprep is widely used in gut microbiome and soil metatranscriptomics research at institutions including Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center.
&nbsp;
For bulk pricing on ZymoBIOMICS DNA/RNA Miniprep kits, bead-beating instrument recommendations, or protocol guidance for low-biomass or high-inhibitor environmental samples,&nbsp;<a href="https://mbpinc.net/contact-us">contact the MBP team</a>.

Spin-column

ZymoBIOMICS™ DNA/RNA Mini Kit (50 Preps.)

The ZymoBIOMICS™ DNA/RNA Miniprep Kit by Zymo Research simultaneously co-purifies DNA and total RNA (≥17 nt, including miRNA) from feces, soil, plant, and environmental samples in 50 preparations. The Zymo-Spin™ IIICG column binds up to 100 µg with a minimum 50 µl elution at A260/A280 >1.8. Also available in an RNA-only format. Kit components stored at room temperature.

MSDS (EU)

MSDS (US)

ZymoBIOMICS™ Fecal reference with TruMatrix Tech (1 ml)

The D6323 by Zymo Research is a 1 mL human fecal reference material with TruMatrix Technology, containing ~10 mg feces/mL, ~6 µg DNA/mL, and ~11 µg RNA/mL from a single homogenized batch of over 2 million identical preparations. Stored in DNA/RNA Shield with fully inactivated pathogens, D6323 serves as a reproducible positive control for 16S amplicon sequencing, metagenomics, and metatranscriptomics workflows.

Characterization

Concentration

Stability

Total DNA Content

Total RNA Content

ZymoBIOMICS™ Gut Microbiome Standard (750 µl)

ZymoBIOMICS Gut Microbiome Standard (D6331) by Zymo Research — 750 µl (10 preps), 21 microbial strains (Bacteria, Fungi, Archaea), ~3.94 × 10⁹ cells/ml, &lt;15% abundance deviation. BSL 1 inactivated. For gut microbiome NGS workflow validation. Stored in 2× DNA/RNA Shield. Made in the USA.

Biosafey

Relative-abundance Deviation

Total Cell Concentration

ZymoBIOMICS™ HMW DNA Standard (50 µl)

D6322 by Zymo Research is a high-molecular-weight (&gt;50 kb) DNA standard containing 5000 ng of genomic DNA from seven bacterial species and one yeast strain at defined theoretical proportions, designed to benchmark third-generation long-read sequencing workflows including Oxford Nanopore and PacBio platforms.

Source

ZymoBIOMICS™ MagBinding Buffer (250 ml)

The D4302-2-250 by Zymo Research is the ZymoBIOMICS MagBinding Buffer in a 250 ml format, used to mediate selective nucleic acid binding to ZymoBIOMICS MagBinding Beads in high-throughput microbiomic and metagenomic DNA/RNA extraction workflows. Sold as a standalone replenishment buffer for ZymoBIOMICS MagBead Kit protocols; made in the USA, Research Use Only.

United States

Texas

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