Zymo Reagents

Plates

96-Well 2.0 ml Deep Well Plate

C2015 by Zymo Research — 96-well, 2.0 mL per well, U-bottom polypropylene deep well plate in SBS standard format (127.80 × 85.50 × 43.60 mm), for automated high-throughput nucleic acid purification. Compatible with Hamilton ML STAR, Tecan Fluent, Beckman Biomek, Opentrons OT-2, and Eppendorf epMotion platforms. Available in 2-plate and 50-plate packs.

Bottom Shape

Capacity

Compatible Instruments

Dimensions (H x W x L)

Material

Manufacturer

96-Well 2.0 ml Deep Well Plate (2 PK)

96-Well 2.0 ml Deep Well Plate (50 pack)

Columns, Plastics & Accessories

96-Well Block

P1001-10 by Zymo Research is a pack of ten 96-Well Blocks made from clear polypropylene, with 2 mL per round-bottom well and a standard-footprint 96-well layout. Compatible with multichannel pipettes and automated liquid handling systems for purification, storage, and assay workflows.

Shipping Condition

Storage Condition

96-Well Block (10 Blocks)

96-Well Block (2 Blocks)

96-Well Block w/ Cover Foil (2 Blocks/Foils)

96-Well Block w/ Cover Foil P1002-2 by Zymo Research — 2 mL round-bottom wells, clear polypropylene, pack of 2 blocks with 2 cover foils. Designed for Zymo spin kit collection workflows. Made in the USA. Ships from MBP INC to USA, Canada, and international labs.

96-Well ELISA Plate (12 x 8-Well Strips)

C2020 by Zymo Research is a 96-well ELISA plate formatted as 12 removable 8-well strips with clear flat-bottom wells, validated for use with the Zymo Research 5-mC DNA ELISA Kit and Quest 5-hmC™ DNA ELISA Kit. The strip format allows partial-run processing, reducing reagent waste in experiments requiring fewer than 96 wells. Made in the USA and available from MBP, a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, and many other institutes across the USA.

96-Well PCR Conversion Plate (2 Plates)

C2008 by Zymo Research is a 96-well non-skirted PCR collection plate (200 µL/well, alphanumeric labels, rimmed wells) used as the eluate collection plate under Zymo Research 96-well column purification kits. Supplied as 2 plates per unit. Made in the USA.

<h2 data-section-id="v1drft" data-start="0" data-end="43">What are cloning chemicals and reagents?</h2>
&nbsp;
Cloning chemicals and reagents cover the enzymes, buffers, and molecular biology-grade chemicals used in DNA fragment preparation, modification, and purification within a cloning workflow, including restriction endonucleases, T4 DNA ligase and other DNA-modifying enzymes (phosphatases, polymerases), reaction buffers, and plasmid extraction reagents. Related entities include restriction enzyme digestion, DNA dephosphorylation/phosphorylation, plasmid DNA purification (column-based and magnetic bead-based), and enzyme unit definitions.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Targeted XJa and XJb strains for faster recovery of proteins.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Mix &amp; Go! solutions for optimized and dependable bacterial transformation.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Culture Media &amp; Supplements used for yeast research, and include YPD Plus and mating pheromones.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Extraction and Lysis Reagents include reliable genomic preparation and yeast protein kits.</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Chromogenic Substrates and Enzymatic Inducers with highly pure X-GAL and IPTG for cloning and blue-white screening.</li>
</ul>
&nbsp;
<h2 data-section-id="1daajgc" data-start="866" data-end="913">How to choose cloning chemicals and reagents</h2>
&nbsp;
Restriction enzyme selection and buffer compatibility
Restriction enzymes require specific reaction buffers, and many modern enzyme lines use a single universal buffer compatible across multiple enzymes to simplify double-digest reactions; confirm buffer compatibility when combining enzymes from different suppliers or using older single-buffer-per-enzyme formulations.
Enzyme unit definitions and reaction scale
Restriction enzyme and ligase activity is defined in units per microliter, with one unit typically defined as the amount needed to digest or ligate a defined quantity of standard substrate DNA under specified conditions in a set time; scale enzyme quantity to the amount of DNA being processed rather than assuming a fixed volume works for all reaction sizes.
Plasmid purification grade
Plasmid DNA extraction reagents range from molecular biology grade, suitable for general cloning and sequencing, to transfection-grade or endotoxin-free formulations required for applications such as lentiviral packaging or mammalian cell transfection, where endotoxin contamination can reduce transfection efficiency or cause cell stress.
Dephosphorylation for preventing vector self-ligation
Alkaline phosphatase treatment of a linearized vector before ligation removes 5' phosphate groups, preventing vector self-ligation and improving the ratio of recombinant clones; this step is particularly useful when the vector and insert share compatible sticky ends that could otherwise religate without insert incorporation.
&nbsp;
<h2 data-section-id="1fljvas" data-start="3459" data-end="3484">Specifications context</h2>
&nbsp;
Restriction enzymes and ligases are supplied at defined unit concentrations (commonly 10&ndash;20 units per microliter for restriction enzymes), with reaction buffers formulated for specific pH and salt conditions optimized for enzyme activity. Plasmid purification kits are differentiated by yield (micrograms of DNA recovered), purity (A260/A280 ratio), and endotoxin level (endotoxin units per microgram), with endotoxin-free kits specifying levels below a defined threshold suitable for transfection applications.&nbsp;
This sub-category is part of MBP's cloning catalog alongside ligation independent cloning kits and transformation kits and competent cells, used downstream of enzyme-based fragment preparation. Labs producing custom lentivirus for immortalization can also review MBP's cell immortalization catalog. MBP's specialist team can help confirm enzyme buffer compatibility and plasmid purification grade for a specific downstream application before order placement.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP today to request a quote for high-purity chemicals and reagents.

Chemicals/Reagents

MSDS

Protocol

Alpha-Factor Mating Pheromone (240 µl)

Alpha-Factor Mating Pheromone (Y1001) by Zymo Research — 240 µl at 10 mM in 0.1 M sodium acetate pH 5.2 (4 mg total); &gt;98% HPLC purity; MW 1684.0 Da. Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr. G1 cell cycle arrest in yeast. Storage at −20°C.

Amino Acid Sequence

Concentration

Molecular Weight

Product Storage

Purity

Usage

<h2 data-section-id="1go10h8" data-start="173" data-end="215">What are classic cell culture media?</h2>
&nbsp;
Classic cell culture media refers to the foundational basal medium formulations that have underpinned mammalian cell culture since the mid-20th century: DMEM (Dulbecco's Modified Eagle Medium), MEM (Minimum Essential Medium), RPMI 1640, Ham's Nutrient Mixtures F-10 and F-12, IMDM (Iscove's Modified Dulbecco's Medium), and the combination formulation DMEM/F-12. Each is manufactured according to its originally published formula, with modifications limited to those necessary for consistent performance and stability of the medium over its shelf life. These media supply the amino acids, vitamins, glucose, salts, and (in most formulations) phenol red pH indicator that form the nutritional backbone of essentially all subsequent specialty, serum-free, and Advanced media derivatives.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Selective Mammalian Antibiotics including Puromycin, Geneticin, and Hygromycin B for stable development of cell lines.</li>
<li dir="ltr" role="presentation">Broad-Spectrum Bactericidal Agents for routine contamination prevention, including Penicillin/Streptomycin and Gentamicin&nbsp;</li>
<li dir="ltr" role="presentation">Prokaryotic Selection Reagents for effective bacterial transformation, including Ampicillin, Kanamycin, Chloramphenicol, and Tetracycline&nbsp;</li>
<li dir="ltr" role="presentation">Antifungal and Antimycotic Solutions to maintain sterile cultures, including combined 100X cocktails and Amphotericin B (Fungizone)&nbsp;</li>
<li dir="ltr" role="presentation">Specialized Small Molecules for targeted biochemical assays, including Ganciclovir, Rapamycin, and Actinomycin D&nbsp;</li>
</ul>
&nbsp;
<h2 data-section-id="1mfajxk" data-start="1218" data-end="1252">How to choose a classic medium</h2>
&nbsp;
DMEM vs MEM
DMEM contains up to four times the amino acid and vitamin concentration of original Eagle's MEM, along with higher glucose levels, making it suitable for cell types with elevated nutrient demands &mdash; DMEM is broadly applicable across mammalian, insect, and plant cell phenotypes including primary cells, stem cells, and transformed lines. MEM remains widely used for monolayer culture of many standard cell types, with optional Hanks' or Earle's salt formulations and a calcium-free modification that permits suspension growth.
RPMI 1640 for suspension and hematopoietic culture
RPMI 1640, developed at the Roswell Park Memorial Institute for culturing human leukemic cells, is optimized for non-adherent cell types &mdash; lymphocytes and other blood cells &mdash; and contains glutathione plus higher biotin, B12, and PABA concentrations than DMEM or MEM. Despite its hematopoietic origin, RPMI 1640 supports a broad range of adherent lines (HeLa, T47D, RENCA, CT26.WT) when properly supplemented.
Ham's F-10/F-12 for CHO and specialized applications
Ham's nutrient mixtures were originally developed for CHO cell clones, HeLa cells, and mouse L-cells, and remain suited to hepatocyte culture, viral fusion experiments, and toxicity assays, with formulations available for use with or without serum. Coon's modification of Ham's F-12 roughly doubles the amino acid and pyruvate content and adds ascorbic acid, developed specifically for hybrid cells produced by viral fusion.
DMEM/F-12 for complex, rich-medium applications
DMEM/F-12 (1:1 mixture) combines DMEM's glucose, amino acid, and vitamin richness with Ham's F-12's zinc, putrescine, hypoxanthine, and thymidine, producing an extremely rich complex medium used for MDCK cells, glial cells, fibroblasts, and human endothelial cells &mdash; and serving as the basal medium for many serum-free Advanced formulations used in organoid and stem cell work.
IMDM for high-density rapid proliferation
IMDM further enriches DMEM with additional amino acids and vitamins, substituting potassium nitrate for ferric nitrate and adding HEPES and sodium pyruvate. IMDM's sodium bicarbonate buffer system (5&ndash;10% CO₂) and enriched composition make it well suited for rapidly proliferating, high-density cultures including Jurkat, COS-7, and macrophage cells.
Liquid vs. powder format
Liquid classic media are ready to use (after appropriate FBS and supplement addition) and include sodium bicarbonate in the formulation. Powdered media are manufactured without sodium bicarbonate, since it tends to off-gas in powder form &mdash; 3.7 g/L sodium bicarbonate must be added upon reconstitution, followed by sterile filtration. Powder formats offer cost and shipping-weight advantages for high-volume labs preparing their own media.
&nbsp;
<h2 data-section-id="17i0pj" data-start="5035" data-end="5061">Specifications context</h2>
&nbsp;
Classic media are manufactured according to originally published formulas, with stability-related modifications documented per product. DMEM's sodium bicarbonate buffer system at 3.7 g/L is standard across liquid formulations; powdered formats require this addition upon reconstitution along with 0.22 &micro;m sterile filtration. As of 2026, DMEM, DMEM/F-12, and RPMI 1640 remain the three most widely specified classic media across academic and biomanufacturing labs, with IMDM increasingly used for high-density suspension applications in bioprocessing.
For recombinant growth factors and cytokines used alongside classic media, see Recombinant Proteins. For supplementation reagents (GlutaMAX, NEAA, sodium pyruvate, HEPES), see Cell Culture Supplements &amp; Additives. Browse Mammalian Cell Culture for the broader category.
<a href="https://mbpinc.net/contact-us">Contact the expert team </a>at MBP to get highly efficient microbial control solutions for your lab.

Antibiotics

Ampicillin Sodium

Zymo Research A1001-ZYM: Ampicillin Sodium solution, 100 mg/mL, ≥98% purity, sterile-filtered (0.2 µm). Working concentration 20–100 µg/mL. Store at –20°C. Ready-to-use for E. coli antibiotic selection. Made in USA. RUO.

Working Concentration

Ampicillin Sodium (100 mg/ml) (5 x 5 ml)

Ampicillin Sodium (100 mg/ml) (5 ml)

<h2>What is DNA/RNA Methylation as a reagent category?</h2>
&nbsp;
DNA/RNA Methylation covers the detection and quantification side of epigenetics: the reagents used to identify which cytosines in a DNA sample are methylated, distinguish 5-methylcytosine from 5-hydroxymethylcytosine, and detect RNA methylation marks like N6-methyladenosine (m6A). Methods range from bisulfite conversion, the historically standard approach for single-base-resolution cytosine methylation mapping, to enzymatic conversion as a less damaging alternative, to antibody-based immunoprecipitation for broad-scale enrichment of methylated DNA regions, to direct ELISA-based quantification of global methylation levels.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/methylation-standards">DNA methylation standards:</a> for accurate quantitative analysis and calibration of methylation levels
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/bisulfite-conversion-kits">Bisulfite conversion kits: </a>for efficient conversion of unmethylated cytosines in DNA methylation studies
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/bisulfite-conversion-reagents-and-buffers">Bisulfite Conversion Reagents and Buffers: </a>for enhanced processing of DNA and flexible workflows
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/non-bisulfite-kits-and-reagents">Non-Bisulfite Kits and Reagents: </a>for alternative epigenetic profiling and methylation analysis methods
</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" aria-level="1">
<a href="https://mbpinc.net/reagents/epigenetics/dna/rna-methylation/elisa-kits-and-reagents" style="color: #236fa1;">ELISA Kits and Reagents:</a> for quantifying sensitive DNA/RNA methylation levels
</li>
</ul>
&nbsp;
<h2>How to choose DNA/RNA methylation analysis reagents</h2>
&nbsp;
Choose bisulfite conversion for single-base-resolution cytosine methylation mapping
Bisulfite conversion converts unmethylated cytosines to uracil while leaving 5-methylcytosine largely unchanged under standard conditions, and after amplification and sequencing, the resulting base-pair difference reveals exactly which cytosines were methylated, making it the approach for single-base-resolution mapping but one that introduces significant DNA damage and degradation.
Consider enzymatic conversion as a less damaging alternative to bisulfite treatment
Enzymatic conversion methods achieve the same cytosine-to-uracil discrimination as bisulfite but through enzymatic rather than harsh chemical reactions, minimizing DNA damage and producing higher-quality libraries from fewer sequencing reads, at potentially higher reagent cost.
Use antibody-based MeDIP for broad methylation enrichment without locus-by-locus resolution
Methylated DNA immunoprecipitation uses an antibody against 5-methylcytosine or 5-hydroxymethylcytosine to enrich methylated DNA regions from a fragmented sample, providing genome-wide enrichment data without the single-base resolution of bisulfite sequencing.
Confirm your method can distinguish 5-mC from 5-hmC if that difference matters for your study
Standard bisulfite conversion cannot reliably distinguish 5-methylcytosine from 5-hydroxymethylcytosine, since both resist bisulfite-mediated deamination under standard conditions, so confirming whether your study requires that distinction helps determine whether a specialized additional step is needed.
Choose ELISA-based kits for rapid, global methylation quantification without sequencing
A 5-methylcytosine ELISA kit provides a rapid, quantitative readout of the global level of DNA methylation in a sample without requiring bisulfite conversion or sequencing, useful for broad-level comparisons across multiple conditions or samples when single-base resolution isn't needed.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Bisulfite sequencing is widely regarded as the gold standard for DNA methylation analysis due to its single-base resolution, but it introduces significant technical challenges for amplification and next-generation sequencing library preparation due to DNA degradation, reduced genome complexity, and alignment complexity from the cytosine-to-uracil base conversion. As of 2026, enzymatic conversion is increasingly recommended over bisulfite conversion for methylation analysis specifically because it minimizes these DNA damage and quality issues, enabling improved preservation and detection of cytosine methylation marks from fewer sequencing reads with no change to downstream analysis pipelines.
<a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> today and get reliable DNA/RNA methylation kits for your lab.

DNA/RNA Methylation

Anti-5-Hydroxymethylcytosine Polyclonal Antibody

A4001-ZYM by Zymo Research — Anti-5-Hydroxymethylcytosine Polyclonal Antibody, rabbit IgG, 1 mg/ml in PBS with 0.05% sodium azide. Validated for hmeDIP, ELISA, immunohistochemistry, and dot blot detection of 5-hmC in DNA from mammalian, plant, insect, and microbial sources. Available in 25, 50, and 200 µg sizes.

Buffer Solution

Isotype

Protein Concentration

Source

Specificity

Storage

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (200 µg)

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (25 µg)

Anti-5-Hydroxymethylcytosine Polyclonal Antibody (1 µg/µl) (50 µg)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl)

A3002-ZYM by Zymo Research — Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21), mouse IgG1, 0.5 µg/µl in PBS with 0.05% sodium azide. Validated for MeDIP, ELISA, and dot blot; specific for 5-mC in single-stranded DNA with no detectable cross-reactivity to 5-hmC or unmodified cytosine. Available in 15–200 µl sizes.

Clone

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (15 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (200 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (30 µl)

Anti-5-Methylcytosine (Clone 7D21) (0.5 µg/µl) (50 µl)

<h2>What are 5-hmC ELISA kits?</h2>
&nbsp;
5-hmC ELISA kits are antibody-based assays formulated specifically to quantify 5-hydroxymethylcytosine, the oxidized derivative of 5-methylcytosine produced by TET family enzymes, in a genomic DNA sample. The assay typically uses denatured single-stranded DNA coated on a plate well, recognized by an anti-5-hmC primary antibody and developed with an HRP-conjugated secondary antibody for a colorimetric readout proportional to the global level of 5-hmC in the sample.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">DNA hydroxymethylation ELISA assay kits for 5-hmC levels quantification</li>
<li dir="ltr" role="presentation">DNA methylation ELISA kits for comparative gene regulation analysis and measurement of 5-mC&nbsp;</li>
<li dir="ltr" role="presentation">DNA standards for accurate methylation and hydroxymethylation quantification&nbsp;</li>
<li dir="ltr" role="presentation">Control DNA sets and assay controls for experimental accuracy, validation, and reproducibility</li>
<li dir="ltr" role="presentation">HRP-based antibodies for the specific detection in ELISA workflows</li>
<li dir="ltr" role="presentation">ELISA coating and reaction buffers for enhanced assay performance and setup</li>
<li dir="ltr" role="presentation">Signal development reagents for quantitative readout and chemiluminescent or colorimetric detection</li>
</ul>
&nbsp;
<h2>How to use 5-hmC ELISA kits</h2>
&nbsp;
Confirm 5-hmC is detectable at relevant levels in your cell type or tissue
5-hmC is most abundant in neurons and embryonic stem cells, where it can constitute up to 20% of total modified cytosines in neuronal DNA, while most cultured cell lines have much lower 5-hmC levels that may fall below a plate-based antibody assay's reliable detection limit. Checking published 5-hmC abundance for your specific cell type helps confirm whether ELISA-level detection is appropriate before committing samples to the assay.
Use a set of mixed methylation standards to build a calibration curve
Running defined mixtures of 5-hmC-containing and unmethylated DNA at known ratios generates a standard curve that lets you convert the raw absorbance signal to an actual global hydroxymethylation percentage, since the assay signal alone cannot be interpreted as an absolute level without this calibration.
Confirm your antibody's specificity for 5-hmC versus 5-mC
Anti-5-hmC antibodies used in these kits are selected for specificity to 5-hydroxymethylcytosine rather than 5-methylcytosine, but published cross-reactivity data for the specific antibody in your kit is worth checking, particularly when studying sample types where both marks are present at similar levels.
Plan your experimental design to include appropriate negative and positive controls
Including a negative control (unmethylated DNA, expected to give background-level signal) and a positive control (5-hmC-containing DNA of known level) in every plate confirms the assay is performing as expected and catches plate-to-plate variability before it affects result interpretation.
Understand ELISA gives global level, not locus-specific 5-hmC information
A 5-hmC ELISA measures the average hydroxymethylation level across all DNA in the sample; it does not reveal which specific CpG sites carry 5-hmC, which requires hMeDIP-seq or TAB-seq for regional or base-level resolution respectively.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
Anti-5-hmC antibodies used in ELISA formats are validated specifically for 5-hydroxymethylcytosine and selected for minimal cross-reactivity with 5-methylcytosine, since distinguishing these two modifications is the primary purpose of a dedicated 5-hmC assay rather than a general methylation assay. As of 2026, 5-hmC ELISA kits remain a standard first-pass tool in studies where many samples need to be screened for differences in global hydroxymethylation level before committing a smaller subset to high-resolution sequencing methods.
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at Molecular Biology Products Inc. (MBP) and find reliable ELISA kits and reagents for your lab.

ELISA Kits & Reagents

Anti-DNA HRP Antibody (100X)

For use with Zymo Research ELISA products

Anti-DNA HRP Antibody (100X) (100 µl)

Anti-DNA HRP Antibody (100X) (200 µl)

<h2>What are epigenetics enzymes and inhibitors?</h2>
&nbsp;
Epigenetics enzymes and inhibitors are the molecular tools for directly studying or modulating the enzymatic machinery that writes, erases, or reads epigenetic marks. The major classes include: DNA methyltransferases (DNMT1, DNMT3A, DNMT3B), which catalyze the addition of a methyl group to cytosine; TET family enzymes (TET1, TET2, TET3), which oxidize 5-methylcytosine to 5-hydroxymethylcytosine; histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add and remove acetyl groups from histone lysine residues; and histone methyltransferases (HMTs) including EZH2, DOT1L, and G9a. Small-molecule inhibitors of HDAC and DNMT enzymes are the most clinically and experimentally advanced of these tools.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Micrococcal nuclease for nucleosome mapping and controlled chromatin digestion applications</li>
<li dir="ltr" role="presentation">DNA shearing enzymes for consistent fragmentation of DNA in sequencing and epigenetic workflows</li>
<li dir="ltr" role="presentation">DNA degradation enzymes for controlled breakdown and analysis of nucleic acids&nbsp;</li>
<li dir="ltr" role="presentation">CpG methylase enzymes for targeted DNA methylation (CpG-specific )&nbsp; in epigenetic modification studies</li>
<li dir="ltr" role="presentation">5-hmC glucosyltransferase for detection and labeling of hydroxymethylcytosine in DNA</li>
<li dir="ltr" role="presentation">SAM (S-adenosylmethionine) cofactors for supporting enzyme activity and methylation reactions</li>
<li dir="ltr" role="presentation">DNA digestion enzymes for genomic DNA processing workflows</li>
<li dir="ltr" role="presentation">dsDNA nuclease systems for controlled cleavage and analysis of double-stranded DNA</li>
</ul>
&nbsp;
<h2>How to choose epigenetics enzymes and inhibitors</h2>
&nbsp;
Choose DNMT inhibitors to globally reduce or deplete DNA methylation in cells
Nucleoside analog DNMT inhibitors like 5-azacytidine and decitabine incorporate into DNA and trap DNMT1, preventing maintenance methylation and causing passive demethylation through DNA replication, while newer non-nucleoside DNMT inhibitors are in development for more selective, non-genotoxic methylation reduction.
Choose HDAC inhibitors to globally increase histone acetylation
HDAC inhibitors including pan-HDAC hydroxamates like vorinostat (SAHA) or class-selective compounds reduce histone deacetylase activity and cause hyperacetylation of histone lysine residues, broadly promoting gene expression at silenced loci. HDAC inhibitors are the most clinically advanced class of epigenetic therapeutics, with multiple FDA-approved compounds.
Use TET enzyme preparations for in vitro 5-hmC oxidation studies
Purified TET1, TET2, or TET3 enzyme preparations allow controlled in vitro oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products, useful for generating defined 5-hmC-containing substrate DNA or studying TET enzyme activity and selectivity.
Choose EZH2 or other HMT inhibitors for studying PRC2-dependent gene silencing
EZH2, the catalytic subunit of polycomb repressive complex 2 (PRC2), catalyzes H3K27 trimethylation (H3K27me3), a histone modification associated with repression of tumor suppressor genes, and EZH2 inhibitors like tazemetostat are used in research to probe the role of this silencing mark.
Combine inhibitors to study epistatic relationships between epigenetic marks
Because DNA methylation, histone acetylation, and histone methylation are mechanistically linked, combining HDAC inhibitors with DNMT inhibitors can produce synergistic effects on gene expression exceeding either alone, and these combination approaches are both experimental tools and the basis for combination epigenetic therapy research.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
HDAC and DNMT inhibitors are the most clinically advanced classes of epigenetic therapeutics, with multiple HDAC inhibitors including vorinostat and romidepsin approved for hematological malignancies, EZH2 inhibitors including tazemetostat having received accelerated approval, and multiple BET inhibitors in clinical trials. As of 2026, CRISPR/dCas9 systems fused with DNMTs, TETs, and HDACs enable locus-specific epigenome editing, extending classic enzyme and inhibitor approaches toward targeted epigenetic modulation.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and find the suitable enzymes and inhibitory reagents for your lab.

Enzymes & Inhibitors

Atlantis Digestion Buffer (1X) (50 ml)

Atlantis Digestion Buffer (1X), SKU E2030-1 by Zymo Research — 50 mL ready-to-use companion buffer for the Atlantis dsDNase (E2030) enzyme. Used for nucleosomal DNA preparation in ChIP, ATAC-seq, and nucleosome positioning workflows. Store at −20°C; part of the EZ Nucleosomal DNA Prep Kit (D5220).

Atlantis dsDNAse (12.5 U)

E2030 by Zymo Research is Atlantis dsDNAse, 12.5&nbsp;U at 0.1&nbsp;U/&micro;l (125&nbsp;&micro;l). Selectively digests dsDNA without degrading ssDNA or RNA. Used for nucleosome mapping and ChIP-seq chromatin fragmentation. Storage: &minus;20&deg;C.