His-Tag Purification Kits

Buffers

MSDS

His-Affinity Gel (14 ml)

His-Affinity Gel (14 mL) — P2003-2 by Zymo Research. Nickel-charged agarose IMAC resin, &ge;15 mg His-tagged protein/mL binding capacity, 50% suspension in 30% ethanol. Bulk replacement gel for His-Spin Protein Miniprep (P2001). Store at 4°C.

Protein Binding Capacity

Manufacturer

Shipping Condition

Storage Condition

His-Binding Buffer (50 ml)

His-Binding Buffer P2003-3 by Zymo Research: 50 ml IMAC binding buffer for His-tagged protein purification in the His-Spin Protein Miniprep Kit. Compatible with E. coli and HEK cell lysates. Purify up to 1 mg of His-tagged protein in ~5 minutes. Store at 4°C. Research use only.

His-Elution Buffer (25 ml)

His-Elution Buffer — P2003-5 by Zymo Research — is a 25 mL ready-to-use elution buffer for histidine-tagged protein purification, designed for use with the His-Spin Protein Miniprep Kit (P2001) and His-Affinity Gel (P2003-2). Manufactured in the USA, it provides a convenient standalone replenishment for labs running His-tag IMAC purification at scale. Order from MBP via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or contact <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Protocol

His-Spin Protein Miniprep™

His-Spin Protein Miniprep P2001 by Zymo Research: 10-prep nickel-agarose IMAC kit purifying up to 1 mg His-tagged protein per prep in ~5 minutes. Compatible with E. coli and HEK cell lysates. Store buffers and gel at 4°C. Research use only.

Affinity Matrix

Binding Capacity

Elution Method

Elution Volume

Principle of Technology

Processing Time

Product Storage

Protein Purity

Required Equipment

Sample Type

His-Spin Protein Miniprep™ (10 Preps.)

His-Spin Protein Miniprep™ (50 Preps.)

His-Wash Buffer (50 ml)

P2003-4 by Zymo Research is the His-Wash Buffer (50 mL) for His-tagged protein purification — 50 mM sodium phosphate pH 7.7, 300 mM NaCl, 50 mM imidazole, 0.03% Triton X-100 — designed to remove weakly bound contaminants from nickel-agarose resin in the His-Spin Protein Miniprep workflow (kits P2001 and P2002). Made in USA.

United States

Texas

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