qPCR/Real-Time PCR

<h2 dir="ltr"> What Are One-Step RT-qPCR Kits?</h2>
 A one-step RT-qPCR kit combines reverse transcription and real-time quantitative PCR in a single tube and buffer, using a temperature-activated reverse transcriptase paired with a hot-start Taq polymerase and a fluorescent detection chemistry - either an intercalating dye such as SYBR Green or a hydrolysis probe system such as TaqMan. The combined format allows room-temperature reaction setup, with reverse transcription and qPCR proceeding sequentially within the same thermal cycling protocol. Choose a SYBR Green one-step RT-qPCR kit for flexible, cost-effective RNA quantification across many targets; choose a probe-based one-step kit when higher specificity or multiplexing of several targets in one well is required.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="rd7qxi" data-start="780" data-end="912">Single-tube reverse transcription and real-time PCR workflows using one-step RT-qPCR kits from ZymoScript and Accuris qMAX</li>
<li data-section-id="1cvnwuk" data-start="913" data-end="1026">SYBR Green and hydrolysis probe-based detection chemistries for RNA quantification and gene expression analysis</li>
<li data-section-id="15d7q7t" data-start="1027" data-end="1119">One-step RT-qPCR master mix systems that reduce sample handling and streamline assay setup</li>
<li data-section-id="b3haux" data-start="1120" data-end="1195">Probe-based RT-qPCR kits suitable for target-specific detection workflows</li>
<li data-section-id="10mg35u" data-start="1196" data-end="1288">Green dye-based RT-qPCR kits for real-time amplification and quantification of RNA targets</li>
</ul>
<h2 dir="ltr"> How to Choose a One-Step RT-qPCR Kit</h2>
 Detection Chemistry SYBR Green-based one-step RT-qPCR kits use an intercalating dye for broad, cost-effective quantification, with specificity driven by primer design. Probe-based one-step kits use TaqMan-style or other sequence-specific probes, offering higher specificity and multiplexing of multiple targets -- including via Scorpions primers or other probe formats -- in a single reaction. Reverse Transcriptase and Activation Mechanism Many one-step RT-qPCR kits use a temperature-activated or aptamer-based reverse transcriptase with dual enzyme-activity control, allowing room-temperature reaction setup without premature activity. Upon heating, both the reverse transcriptase and hot-start Taq polymerase reach their working states in a coordinated activation step. Buffer System Additives Specialised buffer additives -- such as ion combinations that promote specific primer annealing, or compounds that reduce annealing and extension times -- can improve specificity, sensitivity, and overall reaction speed, particularly useful for difficult templates or time-sensitive workflows. Internal Control Options Some one-step RT-qPCR kits offer an optional internal control RNA that can be co-amplified to monitor successful reverse transcription and amplification independently of the target signal, useful for troubleshooting and quality control in diagnostic-style workflows. Instrument Compatibility Confirm reference dye requirements (commonly ROX) and detection channel compatibility against your specific real-time PCR instrument before selecting a kit, since some formulations are offered with and without reference dye for compatibility across different instrument platforms. 
<h2 dir="ltr"> Specifications Context</h2>
 The combined one-step RT-qPCR reaction proceeds through reverse transcription at a temperature suited to the included reverse transcriptase (commonly in the 50-55 &deg;C range for engineered, higher-thermostability enzymes), followed by a polymerase activation step (commonly around 95 &deg;C) that simultaneously inactivates the reverse transcriptase and activates the hot-start Taq polymerase before standard qPCR cycling begins. Internal control RNA, when included, is typically detected in a separate fluorescence channel from the target, allowing simultaneous confirmation of reaction success and quantification of the target&nbsp;from the same well. One-step RT-qPCR kits are typically stored at &minus;20 &deg;C to &minus;30 &deg;C and protected from light; lot-specific CoAs documenting performance and quality control are available on request. 
&nbsp;
<a href="https://mbpinc.net/contact-us">Contact the expert team</a> at MBP today and get optimized One-Step RT-PCR kits for your lab.

One-Step RT-qPCR Kits

MSDS

DataSheet

Protocol

ZymoScript One-Step RT-qPCR Kit (100 rxns)

ZymoScript One-Step RT-qPCR Kit (R3014) by Zymo Research — 100-reaction single-tube RT-qPCR; RNA input 0.1 pg – 5 µg; sensitivity 0.1 pg; cDNA synthesis at 55°C for 10 min. Compatible with SYBR Green and TaqMan. Storage at −20°C.