Spin-column

<h2>What Are Spin-Column Total RNA Kits?</h2>
&nbsp;
Spin-column total RNA kits are the most widely used format for manual RNA purification in molecular biology laboratories. The technology relies on silica membrane chemistry: under chaotropic (guanidinium-based) lysis conditions, RNA adsorbs selectively to the silica membrane of the column; sequential ethanol-based wash steps remove proteins, salts, and gDNA fragments; and a low-ionic-strength buffer or RNase-free water then releases the purified RNA. Standard mini-format kits accept cell or tissue lysate from inputs of 1x10^4-1x10^7 cells or 5-100 mg tissue, bind up to 100 ug RNA, and complete the protocol in 15-30 minutes. The eluted RNA is suitable for RT-PCR, qRT-PCR, RNA sequencing, Northern blot, and hybridization without additional cleanup.&nbsp;Spin-column RNA kits are the default choice for 1-24 samples per run in any lab that does not require automated magnetic-bead processing.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" aria-level="1">
Quick-RNA Microprep and Miniprep Kits for low-volume elution and highly concentrated recovery, providing ultra-clean and phenol-free transcript isolation
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; RNA Kits to bypass tedious phase separation steps, allowing researchers to pump TRIzol-lysed samples directly into spin-columns&nbsp;
</li>
<li dir="ltr" aria-level="1">
Direct-zol&trade; DNA/RNA Parallel Modules are engineered for the non-destructive, simultaneous isolation of DNA and RNA fractions.
</li>
<li dir="ltr" aria-level="1">
ZymoBIOMICS&trade; RNA Mini Kits for uniform microbial profiling featuring host-zeroing lysis mechanics
</li>
<li dir="ltr" aria-level="1">
Quick-RNA&trade; Midiprep Assemblies to capture larger quantities of high-purity total transcripts efficiently, tailored for bulk processing&nbsp;
</li>
</ul>
&nbsp;
<h2>How to Choose Spin-Column Total RNA Kits</h2>
&nbsp;
Column Binding Capacity and Elution Volume
Mini-format columns bind up to 100 ug total RNA and elute in 10-100 uL. For samples with very low RNA content (needle biopsies, laser-capture microdissection sections, small cell numbers &lt;=10^4), choose micro-format columns with 8-12 uL minimum elution volume to maximize concentration. Eluting in &lt;30 uL with high RNA concentration increases sensitivity in downstream RT-qPCR.
Small RNA Retention
This is the most important differentiator between kit brands. Kits using Zymo-Spin column technology with proprietary binding buffers retain miRNA and small RNA species &gt;=17 nt in the total RNA fraction. Competing kits using standard high-ethanol wash chemistry typically lose RNA &lt;200 nt in the wash steps. If your workflow includes small RNA profiling, miRNA RT-qPCR, or small RNA-seq, verify that the kit retains &gt;=17 nt RNA before purchasing.
On-Column DNase Treatment
Included RNase-free DNase I that degrades gDNA on the column during the wash stage eliminates the need for post-elution DNase treatment. This matters for RT-qPCR with primers spanning a small intron, or for RNA-seq where even trace gDNA contamination generates false mapping reads. DNase-treated RNA typically passes a no-RT PCR control at Ct &gt;35.
Sample Type: Cells vs Tissue
Cell lysis buffer volumes and homogenization requirements differ by sample type. Cultured cells lyse directly in lysis buffer with vortexing; tissue samples require homogenization to disrupt cell structure before contact with the lysis buffer. For fibrous tissues (muscle, heart), use a bead-mill homogenizer or rotor-stator; for soft tissues (liver, brain), a disposable pestle or needle shearing is sufficient. Some kits include TissueLyser-compatible bead tubes or QIAshredder-equivalent homogenizer columns.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Spin-column total RNA purity targets: A260/A280 ratio 1.8-2.1; A260/A230 ratio &gt;1.8. RNA integrity number (RIN) from fresh cultured cells or snap-frozen tissue is routinely 8-10 with validated kits. Zymo Research Quick-RNA Mini and Miniprep kits are among the most cited spin-column RNA isolation kits in published RT-PCR and RNA-seq methods; ABM RNA extraction kits offer validated protocols for bacteria, yeast, and fungi not covered by many standard cell/tissue kits. All kits supplied by MBP are Research Use Only (RUO).
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

MSDS

DataSheet

Protocol

Direct-zol RNA Microprep Kits

R2060 by Zymo Research is a microprep-format RNA isolation kit that purifies total RNA, including miRNA (17 nt and above), directly from TRIzol® and equivalent lysis reagents without phase separation, in approximately 7 minutes. Elution volumes as low as 6 µl and RNA quality of A260/A280 >1.8 support sensitive downstream assays. Academic researchers working with scarce cell or tissue samples benefit from maximum recovery in minimal volume.

Compatibility

Equipment

Sample Inactivation

Sample Source

Size Range

Yield

Manufacturer

Shipping Condition

Storage Condition

Direct-zol™ RNA MicroPrep (50 Preps.)

Direct-zol™ RNA MicroPrep (50 Preps.) R2061-A and R2050-1-50

Direct-zol™ RNA MicroPrep (200 Preps.)

Direct-zol™ RNA MicroPrep w/TRI Reagent® (200 Preps.) R2063-A and R2050-1-200

R2070-MSDS

Direct-zol RNA Miniprep Plus Kits

Direct-zol RNA Miniprep Plus Kits by Zymo Research purify total RNA (≥17 nt, up to 100 µg) directly from TRIzol or TRI Reagent in ~7 minutes with on-column DNase I digestion and OD 260/280 purity greater than 1.8. Compatible with animal cells, bacteria, tissue, yeast, and biological fluids. Available in 10 to 200 prep formats.

Direct-zol™ RNA MiniPrep Plus (50 Preps.)

Direct-zol RNA Miniprep Plus

Direct-zol™ RNA MiniPrep Plus w/TRI Reagent® (50 Preps.) R2071 and R2050-1-50

Direct-zol™ RNA MiniPrep Plus (200 Preps.)

Direct-zol™ RNA MiniPrep Plus w/TRI Reagent® (200 Preps.) R2073-A and R2050-1-200

Direct-zol™ DNA/RNA Miniprep Kit

Direct-zol™ DNA/RNA Miniprep Kit by Zymo Research co-purifies genomic DNA (>40 kb, 25 µg capacity) and total RNA (≥17 nt, 50 µg capacity) simultaneously from TRIzol or TRI Reagent-lysed samples in a single column workflow. Ideal for limited samples and integrated multi-omics studies. Available in 10 and 50 prep formats.

Direct-zol™ DNA/RNA Miniprep Kit (50 Preps.)

Direct-zol™ DNA/RNA Miniprep (10 Preps.)

Direct-zol™ DNA/RNA Miniprep Kit w/TRI Reagent® (50 Preps.) R2081-A and R2050-1-50

Direct-zol™ RNA MiniPrep

Direct-zol™ RNA MiniPrep by Zymo Research. 50 µg binding capacity. Elution ≥25 µl. RNA ≥17 nt. 7-minute protocol. Purify total RNA directly from TRI Reagent without phase separation. Available formats: R2051 (50 preps + TRI Reagent), R2051-A (50 preps, alternate TRI bundle), R2050-1-50 (TRI Reagent 50 ml), R2052 (200 preps), R2053 (200 preps + TRI Reagent), R2053-A (200 preps, alternate TRI bundle), R2050-1-200 (TRI Reagent 200 ml).

Direct-zol™ RNA MiniPrep w/TRI Reagent® (50 Preps.) R2051-A and R2050-1-50

Direct-zol™ RNA MiniPrep (200 Preps.)

Direct-zol™ RNA MiniPrep w/TRI Reagent® (200 Preps.) R2053-A and R2050-1-200

Quick-RNA Microprep Kit

Quick-RNA™ Microprep Kit R1050 by Zymo Research isolates total RNA ≥17 nt — including miRNA — from cells, tissue, yeast, plant, and bacteria using Zymo-Spin IC columns, with ≥6 µL elution and on-column DNase I digestion. Compatible with DNA/RNA Shield and RNAlater; lysed samples stable overnight at RT or at –80°C. Available in 50-prep and 200-prep formats from MBP via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Purity

Quick-RNA™ MicroPrep Kit (50 Preps.)

Quick-RNA™ MicroPrep (200 Preps.)

Quick-RNA MiniPrep Kit

Quick-RNA MiniPrep Kit by Zymo Research — total RNA isolation in ~10 min with up to 100 µg binding capacity, A260/A280 &gt;1.8, RNA ≥17 nt. DNase I included. Available as R1054 (50 Preps) and R1055 (200 Preps).

Quick-RNA™ MiniPrep Kit (50 Preps.)

Quick-RNA™ MiniPrep Kit (200 Preps.)

MSDS - R1057/R1058

MSDS - R1057T

Quick-RNA Miniprep Plus Kit

R1057 by Zymo Research is the Quick-RNA™ Miniprep Plus Kit (50 preps) for total RNA isolation — including small RNAs ≥17 nt — from cells, all tissue types, whole blood, FFPE samples, and biological fluids. Zymo-Spin IIICG columns with integrated spin-away filters; DNase I and DNA/RNA Shield included. Typical yield 10–15 µg per 10⁶ cells; purity A260/A280 &gt;1.8. Ready for NGS and RT-qPCR.

Quick-RNA™ MiniPrep Plus Kit (50 Preps.)

Quick-RNA™ MiniPrep Plus (10 Preps.)

Quick-RNA™ MiniPrep Plus Kit (200 Preps.)

Quick-RNA™ MidiPrep (25 Preps.)

Quick-RNA MidiPrep Kit (R1056) by Zymo Research — up to 1 mg RNA yield per prep, ≥17 nt, A260/A280 and A260/A230 &gt;1.8, ≥200 µl elution. 25 Preps, room temperature storage. Optimized for cells and soft tissue.

Optimized Lysis Protocol

ZymoBIOMICS™ RNA Mini Kit (50 Preps.)

ZymoBIOMICS RNA Mini Kit R2001 by Zymo Research isolates total RNA (≥17 nt) from microbiome and environmental samples in 50 preps using ZR BashingBead mechanical lysis. Accepts soil, feces, plant material, biofilms, water, and swabs; 100 µg max binding, A260/A280 &gt;1.8. All components room-temperature stable.

United States

Texas

mbpinc