Bacterial/Fungal RNA/ Spin-column

<h2>What are Spin-Column Bacterial and Fungal RNA Extraction Kits?</h2>
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Spin-column bacterial and fungal RNA extraction kits isolate total RNA from Gram-positive bacteria, Gram-negative bacteria, yeasts, filamentous fungi, algae, and protozoa using ultra-high-density BashingBead mechanical cell wall disruption in a chaotropic lysis buffer, followed by RNA binding to a Zymo-Spin silica-membrane column, on-column washing, and elution in 6-100 ul of RNase-free water - all using a standard microcentrifuge. The complete protocol takes under 15 minutes for a batch of 1-12 samples, requiring only a vortex with a microcentrifuge tube adapter, a microcentrifuge, and standard pipetting equipment. No specialized robotics, vacuum manifolds, or magnetic separation racks are needed. Choose a spin-column bacterial/fungal RNA kit for manual extraction of 1-24 samples per run, when existing automation infrastructure does not support magnetic bead formats, or when the fastest possible turnaround from culture to RT-qPCR data is required.
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<h2 dir="ltr">What you will find:</h2>
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Pinpoint&trade; Slide RNA Isolation System for targeted extraction of total RNA directly from localized tissue sections on microscope slides
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Quick-RNA&trade; Fungal/Bacterial MicroPrep Kit for highly concentrated, low-volume recovery of transcripts utilizing micro-elution spin-column matrices&nbsp;
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ZR Fungal/Bacterial RNA MiniPrep for precise cell-wall shattering of yeast and recalcitrant spores&nbsp;
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YeaStar&trade; RNA Kit efficiently permeabilizes tough yeast cell walls.&nbsp;
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<h2>How to Choose a Spin-Column Bacterial/Fungal RNA Kit</h2>
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Miniprep vs. Microprep Format
The Zymo Quick-RNA Fungal/Bacterial Miniprep processes up to 2x10^8 bacteria or 2x10^7 fungi per column, eluting in 25-100 ul - the standard choice for typical 1-5 ml bacterial cultures. The Microprep handles smaller inputs (up to 1x10^7 bacteria) and elutes in as little as 6 ul, concentrating RNA for sensitive applications such as single-cell-equivalent samples or very low-input transcriptomics.
Bead Size for Target Organism
Pre-mixed 0.1 mm + 0.5 mm BashingBead tubes in Zymo kits handle bacteria (0.1 mm) and fungi (0.5 mm) without selecting bead size. For pure Gram-negative bacterial cultures that do not require bead beating, direct lysis in buffer is available as a faster protocol option that skips the bead-beating step entirely.
On-Column DNase I Treatment
Include DNase I for all RT-qPCR and RNA-seq applications. Bacterial gDNA is abundant and co-purifies efficiently under silica-membrane binding conditions; without DNase treatment, gDNA contamination produces false amplification in RT-qPCR assays targeting genes with no introns. DNase I Reaction Mix is included in Zymo Quick-RNA Fungal/Bacterial kits.
Elution Volume
Elute in 25-50 ul for standard RT-qPCR (2-5 ul per reaction provides 5-25 RNA equivalents per assay). For RNA-seq library preparation requiring &gt;=200 ng input, use 50-100 ul elution volume to recover more total RNA mass; then measure concentration by Qubit and adjust for library prep.
Storage of Extracted RNA
Store spin-column bacterial RNA at -80C in single-use aliquots; mRNA from bacteria is AU-rich and degrades rapidly through multiple freeze-thaw cycles. Use within 6 months for transcriptomic applications; RT-qPCR targets with high expression levels remain quantifiable for 12+ months at -80C.
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<h2>Specifications Context</h2>
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Target quality metrics for spin-column bacterial/fungal RNA: A260/A280 &gt;= 1.8, A260/A230 &gt;= 1.5, and RIN &gt;= 7 from log-phase cultures processed within 60 seconds of harvest. Zymo Research Quick-RNA Fungal/Bacterial kits use the same Zymo-Spin column technology found across the Quick-RNA product line and are validated for inhibitor-free RNA suitable for NGS, RT-PCR, microarray, and hybridization. The Quick-RNA Fungal/Bacterial Miniprep is one of the most widely used spin-column formats for bacterial and fungal RNA in academic molecular microbiology research, with validated protocols for S. cerevisiae, C. albicans, A. fumigatus, and standard Gram-positive and Gram-negative species.&nbsp;
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<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for kit selection guidance, bulk pricing, and Zymo Research protocol adaptation for non-standard organisms.

Spin-column

MSDS - R1003

MSDS - R1007

Protocol - R1003

Protocol - R1007

R1003-Protocol

Pinpoint™ Slide RNA Isolation System I

Pinpoint Slide RNA Isolation System I (R1003) by Zymo Research — 50-prep RNA isolation from fresh/frozen tissue sections on glass slides. Recovers &ge;17 nt RNA (incl. microRNA), elutes in &ge;6 µL, A260/A280 &gt;1.8, up to 10 µg/prep. All components stored at room temperature.

Equipment

Purity

Sample Source

Size Range

Yield

Manufacturer

Shipping Condition

Storage Condition

Pinpoint™ Slide RNA Isolation System I (50 Preps.)

Pinpoint™ Slide RNA Isolation System II (50 Preps.)

RNA Digestion Buffer (1.2 ml)

RNA Extraction Buffer (12 ml)

RNA Extraction Buffer (3 ml)

RNA Wash Buffer (12 ml)

RNA Wash Buffer (24 ml)

RNA Wash Buffer (48 ml)

RNA Wash Buffer (6 ml)

MSDS

Protocol

Quick-RNA™ Fungal/Bacterial MicroPrep Kit (50 Preps.)

Quick-RNA™ Fungal/Bacterial MicroPrep Kit R2010 by Zymo Research — 50 preps, RNA ≥17 nt, 10 µg binding, ≥6 µL elution, ≤10 min. ZR BashingBead Lysis Tubes for mechanical disruption of fungal (Candida, Aspergillus, Saccharomyces) and bacterial cell walls. A260/A280 &gt;1.8. Order via <a href="https://www.mbpinc.net/quickorder">Quick Order</a> or <a href="mailto:customerservice@mbpinc.net">customerservice@mbpinc.net</a>.

Applicable For

Elution Volume

Processing Time

Processing Volume

Type

YeaStar™ RNA Kit (40 Preps.)

YeaStar™ RNA Kit R1002 by Zymo Research — 40-prep yeast RNA isolation kit, ≤25 µg yield, A260/A280 ≥1.8, 30-min protocol, compatible with S. cerevisiae, Candida, Aspergillus, Pichia, and Schizosaccharomyces. Ships from MBP INC to USA, Canada, and international labs.

DataSheet

ZR Fungal/Bacterial RNA MiniPrep (50 Preps.)

ZR Fungal/Bacterial RNA MiniPrep (R2014) by Zymo Research isolates up to 50 µg of total RNA (≥17 nt) from gram-positive bacteria, fungi, yeast, spores, and pollen in 10 minutes or less using a bead-beating lysis step followed by spin-column purification. Purity exceeds A260/A280 of 1.8, and purified RNA is ready for RT-qPCR, RNA-seq, and microarray applications. 50-prep format.

United States

Texas

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