Plasmid DNA Extraction Kits

<h2>What are plasmid miniprep kits?</h2>
&nbsp;
A plasmid miniprep kit uses SDS/alkaline lysis to break open pelleted E. coli cells from a small culture volume (1-10 ml), followed by binding to a silica-membrane spin column, washing, and elution, separating plasmid DNA from genomic DNA, RNA, and protein in roughly 10-15 minutes per prep. Related entities include Buffer P1/P2/P3-style alkaline lysis reagents, color-coded lysis dyes for visual confirmation of complete lysis and neutralization, RNase A for RNA removal, and downstream applications including transformation, restriction digestion, PCR, and sequencing. Choose a miniprep kit for routine, low-volume plasmid prep (cloning screens, sequencing checks, restriction digests) where yields up to roughly 20-100 ug per column are sufficient.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Pellet-free and classic plasmid miniprep kits for high-yield bacterial isolation using rapid spin-column workflows</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-throughput 96-well plasmid plates optimized for vacuum manifold processing and parallel automated screening&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Specialized yeast plasmid miniprep systems for fragile fungal hosts using cell-wall digesting chemistries</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">BAC/PAC and large construct isolation units for safer purification of high-molecular-weight vectors</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid extraction reagents for sensitive mammalian cell lines, ensuring transfection-grade DNA purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid miniprep kit</h2>
&nbsp;
Standard vs. high-yield chemistry
Standard silica spin-column minipreps typically recover up to about 20 ug per column, while high-yield formulations using modified alkaline lysis and novel binding chemistry can reach up to 100 ug at concentrations up to 3 ug/ul from the same small culture volume. If your downstream application (e.g., sequencing multiple samples from one prep, or transfection requiring more DNA per prep) benefits from a higher concentration, a high-yield kit reduces the need to scale up to midiprep.
Plasmid copy number and culture volume planning
High-copy plasmids (pUC-based vectors) can saturate a miniprep column from as little as 3 ml of culture, yielding around 20 ug, while low-copy plasmids (pBR322, pET, cosmids) may yield under 2 ug from the same volume and benefit from doubling culture volume to 5-10 ml. Identify your vector's origin of replication before choosing a standard culture volume, since low-copy vectors at standard volumes can produce yields too low for some downstream steps.
Color-coded lysis buffers for visual QC
Some miniprep kits include color-coded lysis and neutralization buffers that allow visual confirmation of complete cell lysis (color change) and proper neutralization before loading the column, helping catch incomplete-lysis failures before they waste a column. This visual QC step is particularly useful for new users or when troubleshooting inconsistent yields across a batch of preps.
Endotoxin-free and large-construct options
For transfection-sensitive downstream applications, endotoxin-free miniprep kits add a dedicated wash step without significantly extending the protocol time; for large plasmids, cosmids, or BAC/YAC/PAC constructs, some miniprep kits are validated up to approximately 200 kb, while standard kits are optimized for constructs up to roughly 25 kb. Confirm both endotoxin status and size compatibility match your specific construct and downstream use before relying on a standard miniprep.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for plasmid miniprep selection are expected yield relative to your plasmid's copy number and culture volume, whether color-coded buffers aid visual QC, and whether endotoxin-free or large-construct compatibility is needed for your downstream application. A standard miniprep yielding up to roughly 20-25 ug in 10-15 minutes from 1-10 ml of culture covers most routine cloning workflows, while high-yield kits up to 100 ug reduce the need to scale up for moderately DNA-hungry applications. Labs running both daily cloning screens and occasional larger-scale prep work typically keep <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;on hand alongside&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;for when miniprep yields aren't sufficient. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>&nbsp;cover larger-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.&nbsp;
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Miniprep Kits

MSDS

7X Lysis Buffer (Blue)

D4036-3 by Zymo Research — Endo-Wash Buffer for endotoxin removal during plasmid DNA purification with the Zyppy Plasmid Miniprep and ZR Plasmid Miniprep - Classic kits. Room temperature storage. Available in 6 ml, 15 ml, 30 ml, 60 ml, 120 ml, 160 ml, and 240 ml formats. Yields ultra-pure, endotoxin-reduced plasmid DNA.

Manufacturer

7X Lysis Buffer (12 ml) (Blue)

7X Lysis Buffer (30 ml) (Blue)

7X Lysis Buffer (48 ml) (Blue)

7X Lysis Buffer (6 ml) (Blue)

7X Lysis Buffer (60 ml) (Blue)

Neutralization Buffer (Yellow)

D4036-2-100 by Zymo Research is a 100 ml Neutralization Buffer (Yellow) for the alkaline lysis neutralization step in Zyppy Plasmid Miniprep Kit workflows (D4019/D4036/D4020/D4037). The yellow color provides visual confirmation of complete neutralization; the clear lysate is then loaded directly onto the column without centrifugation. Sold as a standalone refill; stored at room temperature.

Neutralization Buffer (100 ml) (Yellow)

Neutralization Buffer (160 ml) (Yellow)

Neutralization Buffer (20 ml) (Yellow)

Neutralization Buffer (200 ml) (Yellow)

Neutralization Buffer (40 ml) (Yellow)

Protocol

ZR BAC DNA Miniprep Kit

D4048 by Zymo Research — ZR BAC DNA Miniprep Kit (25 preps) for rapid isolation of BAC, PAC, fosmid, and cosmid DNA up to ~200 kb from E. coli. 15-minute protocol; elution ≥ 10 µl; yield up to 10 µg per prep; purity A260/A280 ≥ 1.8, A260/A230 ≥ 2.0. Includes colored buffer system for visual lysis confirmation.

Applicable For

Elution Volume

Equipment

Processing Time

Purity

Size Range

Yield

Shipping Condition

Storage Condition

ZR BAC DNA Miniprep Kit (25 Preps.)

ZR BAC DNA Miniprep Kit (100 Preps.)

Product Literature

ZR Plasmid Miniprep - Classic

ZR Plasmid Miniprep Classic D4015 by Zymo Research isolates up to 25 µg of plasmid DNA from up to 5 mL of overnight E. coli culture using a traditional three-buffer alkaline lysis protocol in approximately 15 minutes, with A260/280 ≥1.8 purity and ≤50 EU/µg endotoxin. 100 preps per kit.

Endotoxin Levels

ZR Plasmid Miniprep - Classic (100 Preps.)

ZR Plasmid Miniprep - Classic (400 Preps.)

ZR Plasmid Miniprep-Classic (800 Preps.)

<h2>What are plasmid midiprep kits?</h2>
&nbsp;
A plasmid midiprep kit scales SDS/alkaline lysis and silica-membrane column binding (or anion-exchange resin with gravity flow) to 150-300 ml of overnight E. coli culture, recovering 0.5-2 mg of plasmid DNA with lysate clearing accomplished by high-speed centrifugation followed by a clearing column or filtration step, completable in as little as 30 minutes for some spin/vacuum formats. Related entities include high-speed centrifugation for lysate clearing, NucleoSnap-type midi formats, endotoxin removal washes, and downstream applications including moderate-scale transfection and multi-reaction restriction/sequencing work. Choose midiprep when miniprep yields (up to ~25-100 ug) aren't sufficient, but full maxiprep-scale culture volume (100-500 ml) and processing time aren't necessary.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" role="presentation">Rapid 25-minute midiprep kits to bypass lengthy gravity-flow precipitation steps utilizing accelerated column dynamics</li>
<li dir="ltr" role="presentation">Vacuum-assisted syringe-filter plasmid midi modules engineered for rapid and debris-free clearing of lysates without manual pelleting</li>
<li dir="ltr" role="presentation">Endotoxin-free intermediate purification systems for sensitive mammalian cell screening by delivering transfection-grade constructs</li>
<li dir="ltr" role="presentation">High-purity silica spin-column configurations optimized for elution of high-copy vectors&nbsp;</li>
<li dir="ltr" role="presentation">Advanced anion-exchange gravity-flow assemblies provide template purity for downstream sequencing.</li>
</ul>
&nbsp;
<h2>How to choose a plasmid midiprep kit</h2>
&nbsp;
When midiprep fits between mini and maxi
Midiprep format is the right choice when your downstream application needs more DNA than a miniprep provides (roughly 20-100 ug depending on chemistry) but doesn't require the largest maxiprep-scale yields (up to 1 mg or more); 0.5-2 mg from 150-300 ml culture covers many moderate-scale transfection experiments and multi-replicate downstream reactions. If you find yourself running multiple minipreps to pool DNA for a single transfection, a single midiprep is often more efficient.
Lysate clearing method
Midiprep kits clear lysate using high-speed centrifugation followed by a clearing column (spin or vacuum format) or filtration through folded filters in anion-exchange systems; spin/vacuum clearing formats can complete the full protocol in as little as 30 minutes, while gravity-flow anion-exchange systems may take longer but avoid centrifugation steps for the binding/wash/elute portion. Match the clearing method to your lab's available centrifuge capacity (rotor size for 150-300 ml tubes) and time constraints.
Endotoxin-free midiprep options
For transfection of sensitive cell types, endotoxin-free midiprep kits using anion-exchange chemistry can achieve endotoxin removal in a unique washing step without additional incubations, suitable for transfection of highly sensitive cells, vaccination research, or gene therapy applications. If your midi-scale prep feeds directly into sensitive-cell transfection, confirm the kit's endotoxin specification (e.g., below 0.1 EU/ug) against your cell type's documented sensitivity.
Plasmid copy number and culture volume within the midi range
As with miniprep, plasmid copy number affects yield within the midiprep culture volume range - low-copy plasmids may need culture volumes toward the higher end (300 ml) to reach typical midi yields, while high-copy plasmids may reach 1-2 mg even at 150 ml. Plan culture volume within the midi range based on your specific vector rather than defaulting to the kit's maximum stated volume.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for midiprep selection are whether 0.5-2 mg from 150-300 ml culture meets your downstream DNA quantity needs without over- or under-scaling, lysate clearing method (centrifugation-based spin/vacuum vs. gravity-flow anion-exchange), and endotoxin status for transfection-sensitive applications. A midiprep completing in as little as 30 minutes via spin/vacuum clearing offers a time advantage over gravity-flow systems while still avoiding the larger culture volumes of maxiprep. Labs whose miniprep yields are consistently insufficient for their transfection protocols typically move to <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;before considering&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/maxiprep-kits">maxiprep kits</a>, which require substantially more culture volume and processing time. The parent&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>&nbsp;cover smaller-scale options, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a> link to chromosomal DNA workflows.
&nbsp;
<section class="mbp-contact-cta">
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Midiprep Kits

DataSheet

MSDS - D4213

MSDS - D4213-1

MSDS - D4213-2

MSDS - D4213-3

ZymoPURE - Express™ Plasmid Midiprep Kit

D4213 by Zymo Research: ZymoPURE-Express™ Plasmid Midiprep Kit, 25 preps. 15-minute direct lysis protocol from 25 mL E. coli culture. Yield 100–200 µg; ≤1 EU/µg endotoxin; up to 200 kb. Syringe Filter-X included. Made in USA.

Binding Capacity

Culture Input

Format

Recommended Sample Volume

Yield 

ZymoPURE - Express™ Plasmid Midiprep Kit (25 Preps.)

ZP Express Lysis Buffer (210 ml)

ZP Express Neutralization Buffer (260 ml)

ZP Express Binding Buffer (260 ml)

<h2>What Are Gigaprep Kits?</h2>
&nbsp;
Gigaprep kits are alkaline-lysis-based plasmid purification systems scaled for bacterial cultures of 1-2.5 L, yielding 10-25 mg of plasmid DNA per preparation. The purified DNA is endotoxin-free (typically &lt;=0.1 EU/ug), concentrated to up to 6 ug/ul, and compatible with demanding downstream workflows including mammalian cell transfection, in vitro transcription, gene therapy vector production, and automated sequencing. Unlike miniprep or maxiprep kits, a gigaprep processes sufficient DNA to support multi-well transfection screens, viral vector packaging batches, or long-running in vitro assays from a single prep.&nbsp;Choose a gigaprep kit when your protocol requires more than 2-5 mg of transfection-grade plasmid DNA in a single preparation.
&nbsp;
<h2 dir="ltr">What you will find:</h2>
&nbsp;
<ul>
<li data-section-id="1q3vh4q" data-start="25" data-end="147">Large-scale plasmid DNA purification kits, including the ZymoPURE&trade; II Plasmid Gigaprep Kit, for high-yield DNA recovery.</li>
<li data-section-id="epfgfk" data-start="148" data-end="266">Gravity-flow purification systems that eliminate the need for vacuum manifolds and centrifugation during processing.</li>
<li data-section-id="hdtzmb" data-start="267" data-end="364">Advanced anion-exchange column technology for efficient removal of contaminants and impurities.</li>
<li data-section-id="1ti4gf8" data-start="365" data-end="467">Transfection-grade plasmid DNA preparation solutions suitable for demanding downstream applications.</li>
<li data-section-id="82zytf" data-start="468" data-end="598" data-is-last-node="">High-capacity plasmid isolation products designed for research, protein expression, and large-scale molecular biology workflows.</li>
</ul>
&nbsp;
<h2>How to Choose Gigaprep Kits</h2>
&nbsp;
Purification Chemistry
Anion-exchange resin kits (gravity-flow or vacuum column) use charged resin to bind nucleic acids and separate plasmid from chromosomal DNA, RNA, and endotoxin by salt gradient. Spin-column formats such as the ZymoPURE II Gigaprep use a silica-based membrane with specialized endotoxin-removal steps, completing the prep in ~45 minutes without gravity-flow wait times. Choose anion-exchange for the highest A260/A280 purity when supercoiling integrity is critical; choose spin-column when throughput and speed are the priority.
Endotoxin Removal Requirement
Endotoxin specification matters most for the transfection of primary cells, dendritic cells, or endotoxin-sensitive in vivo models. Kits rated at &lt;=0.1 EU/ug are considered endotoxin-free for most transfection applications. If you work with standard transformed cell lines (HEK293, CHO), standard endotoxin reduction is usually sufficient; for primary neurons, immune cells, or gene therapy studies, confirm the &lt;=0.1 EU/ug spec before selecting a kit.
Starting Culture Volume
Most gigaprep kits are optimized for 1.2-2.5 L of LB or terrific broth (TB). TB medium typically yields 30-50% more plasmid per liter than LB for high-copy vectors. Confirm whether your kit protocol is calibrated for LB or TB to ensure the binding column is not overloaded, which degrades purity.
Plasmid Copy Number
High-copy plasmids (&gt;100 copies/cell, pUC-based) approach theoretical yield limits easily; scale down culture volume if needed to avoid column overload. Low-copy plasmids (bacterial artificial chromosomes, BACs, PACs, YACs) require the full 2-2.5 L and benefit from kits specifically validated for low-copy applications.
Supercoiling Retention
Supercoiled plasmid is the biologically active isoform preferred for transfection and in vitro transcription. Validated gigaprep kits preserve &gt;85% supercoiled plasmid; avoid protocols with aggressive vortexing or extended lysis to protect this fraction.
&nbsp;
<h2>Specifications Context</h2>
&nbsp;
Gigaprep eluates are typically quantified by A260 absorbance and evaluated for purity by A260/A280 ratio (target 1.8-2.0) and A260/A230 ratio (target &gt;1.8). Endotoxin is measured by LAL (Limulus amebocyte lysate) assay; a spec of &lt;=0.1 EU/ug is the accepted threshold for transfection-grade plasmid. As of 2025, the most widely adopted spin-column gigaprep format yields DNA concentrated to 4-6 ug/ul, reducing downstream volume requirements for transfection. All kits supplied through MBP are for Research Use Only (RUO) and are not classified as IVD or medical-device reagents.
<section class="mbp-contact-cta">
&nbsp;
MBP's specialist team can help match kit chemistry, format, and throughput to your sample type and downstream application.&nbsp;<a href="https://mbpinc.net/contact-us">Contact the MBP team</a> for personalized guidance.
</section>

Gigaprep Kits

ZymoPURE™ II Plasmid Gigaprep Kit (5 Preps.)

ZymoPURE™ II Plasmid Gigaprep Kit D4204 by Zymo Research purifies up to 25 mg of endotoxin-free (≤0.025 EU/µg), transfection-ready plasmid DNA from up to 2.5 L of E. coli culture in 45 minutes — no alcohol precipitation required. 5 preps per kit.

<h2>What are plasmid maxiprep kits?</h2>
&nbsp;
A plasmid maxiprep kit scales SDS/alkaline lysis with silica-membrane spin columns, anion-exchange resin with gravity flow, or fully automated cartridge-based processing to 100-500 ml of overnight E. coli culture, recovering up to 400 ug per spin-column prep or 1-1.4 mg with high-yield anion-exchange chemistry, completable in 35-75 minutes depending on format. Related entities include EndoFree-type endotoxin removal for transfection, automated processors that eliminate manual centrifugation, and downstream applications including large-scale transfection, in vitro transcription/translation, and multiple parallel experiments. Choose Maxiprep when your application needs the largest routine plasmid yields (hundreds of micrograms to over 1 mg) from a single overnight culture.
&nbsp;
<h2>What you will find:</h2>
&nbsp;
<ul>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Ultrafast 25-minute maxiprep kits drastically reduce processing times using specialized column technology&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Syringe-filter plasmid maxi units rapidly clear dense bacterial lysates using vacuum-free clarification systems&nbsp;</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Endotoxin-free plasmid maxiprep systems for delivering transfection-grade DNA in sensitive mammalian cell lines</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">High-performance silica-column vectors optimized for maximum concentration and recovery of low and high-copy plasmids</li>
<li dir="ltr" style="font-family: arial, helvetica, sans-serif;" role="presentation">Gravity-flow anion-exchange assemblies without manual centrifugation steps, ensuring chromatography-level purity&nbsp;</li>
</ul>
&nbsp;
<h2>How to choose a plasmid maxiprep kit</h2>
&nbsp;
When maxiprep is the right scale
A maxiprep should be used when purifying plasmid from an overnight bacterial culture with a volume between 100-500 ml, when downstream needs exceed midiprep's 0.5-2 mg range - for example, large-scale transfection across many plates, in vitro transcription/translation requiring substantial template DNA, or banking DNA for multiple future experiments from one culture. If midiprep yields already meet your needs, the larger culture volume and processing time of maxiprep may not be justified.
Spin-column vs. anion-exchange vs. automated chemistry
Spin-column maxipreps (e.g., 60-minute procedures) recover up to roughly 400 ug per prep, while anion-exchange or high-yield chemistries can reach 1-1.4 mg from comparable culture volumes; automated cartridge-based processors can deliver endotoxin-free DNA in under 75 minutes without manual centrifugation, pouring in culture, and pressing start. Match chemistry to both your yield target and available equipment - automated processors reduce hands-on time but represent a larger equipment investment.
Endotoxin levels at maxi scale
Endotoxin concentrations tend to be higher in larger-scale preparations - standard maxipreps have been reported at 11-16 EU/ug, while EndoFree-type kits target below 0.1 EU/ug through additional washing, and "substantially endotoxin-free" is sometimes defined as under 100 EU/ug for less stringent applications. For transfection of highly sensitive cells, vaccination research, or gene therapy applications, confirm the maxiprep kit's specific endotoxin target rather than assuming "maxiprep" implies low endotoxin by default.
Lysate clearing and centrifuge requirements
Maxi-scale lysate clearing typically requires high-speed centrifugation of the full culture volume (100-500 ml) followed by a clearing column or filtration step; gravity-flow anion-exchange systems use folded filters to avoid this centrifugation, while automated processors handle clearing internally. Confirm your centrifuge has rotors compatible with 100-500 ml culture volumes before committing to a centrifugation-based maxiprep format.
&nbsp;
<h2>Specifications context</h2>
&nbsp;
The key specs for maxiprep selection are expected yield (400 ug for spin-column vs. 1-1.4 mg for high-yield/anion-exchange chemistry), endotoxin target relative to your transfection application's sensitivity, and lysate-clearing method relative to your centrifuge's capacity for 100-500 ml culture volumes. A maxiprep should specifically be used for culture volumes in the 100-500 ml range; smaller volumes are better served by <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/midiprep-kits">midiprep kits</a>&nbsp;or&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits/miniprep-kits">miniprep kits</a>. Labs running large-scale transfection or in vitro transcription/translation programs typically standardize on either high-yield anion-exchange maxipreps or automated processors to balance yield, endotoxin level, and hands-on time. The parent <a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/plasmid-dna-extraction-kits">plasmid DNA extraction kits category</a>&nbsp;covers the full mini-to-maxi range, while the broader&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits">nucleic acid extraction kits hub</a>&nbsp;and&nbsp;<a href="https://mbpinc.net/reagents/molecular-biology/nucleic-acid-extraction-kits/dna-extraction-kits/genomic-dna">genomic DNA extraction kits category</a>&nbsp;link to chromosomal DNA workflows. Use MBP's&nbsp;<a href="https://mbpinc.net/quickorder">Quick Order tool</a>&nbsp;for case-quantity pricing on maxiprep kits or&nbsp;<a href="https://mbpinc.net/contact-us">contact the specialist team</a>&nbsp;for guidance on endotoxin levels and automation options for your transfection program.
<section class="mbp-contact-cta"></section>
&nbsp;
Scale-up workflow efficiency with MBP&rsquo;s dependable macro-extraction plasmid technologies. <a href="https://mbpinc.net/contact-us">Contact the expert team</a> today to find the perfect match for your lab.

Maxiprep Kits

Data Sheet

ZymoPURE™ II Plasmid Maxiprep Kit

ZymoPURE™ II Plasmid Maxiprep Kit D4202 by Zymo Research purifies up to 3 mg of endotoxin-free (≤0.025 EU/µg), transfection-ready plasmid DNA from up to 150 mL of E. coli culture in ≤18 minutes, with A260/230 ≥2.0 purity. Supports plasmids up to 200 kb.

ZymoPURE™ II Plasmid Maxiprep Kit (10 Preps.)

ZymoPURE™ II Plasmid Maxiprep Kit (20 Preps.)

D4201 - MSDS

D4201 (D4200/D4201)

ZymoPURE™ II Plasmid Midiprep Kit

D4201 by Zymo Research: ZymoPURE™ II Plasmid Midiprep Kit, 50 preps. Processes ≤50 mL E. coli culture; yields 200–400 µg; ≤18 min protocol. Endotoxin ≤0.025 EU/µg (EndoZero II) or ≤1 EU/µg standard. Up to 200 kb; A260/A280 ≥1.8. Made in USA.

ZymoPURE™ II Plasmid Midiprep Kit (50 Preps.)

ZymoPURE™ II Plasmid Midiprep Kit (25 Preps.)

MSDS - D4208T

MSDS - D4209/D4210/D4211/D4212

ZymoPURE™ Plasmid Miniprep Kit

D4208T ZymoPURE Plasmid Miniprep Kit by Zymo Research — 10 preps; 15-min protocol; up to 100 &micro;g yield; &le;1 EU/&micro;g endotoxin; 25 &micro;l elution; A260/A280 &ge;1.8; plasmids to ~200 kb.

Sample Volume

ZymoPURE™ Plasmid Miniprep Kit (10 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (50 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (100 Preps.)

ZymoPURE™ Plasmid Miniprep Kit (400 Preps.)

ZymoPURE Plasmid Miniprep Kit (800 Preps.)

ZymoPURE™-96 Plasmid Miniprep Kit

D4214 by Zymo Research is the ZymoPURE™-96 Plasmid Miniprep Kit for processing 96 minipreps simultaneously in ≤60 minutes. Yields up to 100 µg transfection-grade plasmid (≤1 EU/µg, A260/A280 ≥1.8) per well in 125 µL from up to 5 mL E. coli culture. Kit supplies 2 × 96 preps.

Culture Input 

ZymoPURE™-96 Plasmid Miniprep Kit (2 x 96 Preps.)

ZymoPURE™-96 Plasmid Miniprep Kit (4 x 96 Preps.)

ZymoPURE 96 Starter Package (4 x 96 preps + 1 EZ-Vac 96 Vacuum Manifold)

Zymoprep™ Yeast Plasmid Miniprep I

D2001 by Zymo Research is the Zymoprep™ Yeast Plasmid Miniprep I, a 100-prep precipitation-based kit for isolating plasmid DNA from S. cerevisiae, C. albicans, S. pombe, and other Zymolyase-susceptible yeasts. Processes ≤1.5 ml culture per prep; elutes ≥35 µl with yields of 0.01–0.3 µg for 2-micron plasmids. DNA is ready for PCR, transformation, and hybridization. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

Zymoprep™ Yeast Plasmid Miniprep I (100 Preps.)

Solution 1 Digestion Buffer (15 ml)

Solution 2 Lysis Buffer (15 ml)

Solution 3 Neutralizing Buffer (15 ml)

Zymoprep™ Yeast Plasmid Miniprep II

D2004 by Zymo Research — Zymoprep Yeast Plasmid Miniprep II, 50 preps. Isolates plasmid DNA from ≤1.5 mL yeast culture using Zymolyase digestion and Zymo-Spin I silica columns; elution ≥10 µl; typical yield 0.01–0.3 ng. Compatible with S. cerevisiae, C. albicans, S. pombe, and ~15 additional Zymolyase-susceptible genera. Yields ~5× more plasmid than Miniprep I.