DNA Cleanup Kits

<h2>What are DNA Cleanup Kits?</h2>
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DNA cleanup kits purify and concentrate pre-extracted or pre-amplified DNA by removing enzymes, salts, organic solvents (phenol, chloroform), unincorporated nucleotides, RNA, detergents, and other contaminants from DNA solutions using silica-membrane spin columns with a bind-wash-elute protocol. Unlike PCR cleanup kits that are specifically optimized to remove primers and dNTPs from PCR reactions, DNA cleanup kits accept broader input chemistries - including post-phenol-chloroform extraction DNA, genomic DNA in high-salt lysis buffers, restriction digest reactions, and dilute DNA solutions requiring concentration - and elute DNA in minimal volumes (as little as 6 ul) for immediate use in downstream applications. Use a DNA cleanup kit whenever your DNA preparation contains inhibitors incompatible with downstream enzymatic reactions, when residual phenol must be removed, or when dilute DNA must be concentrated into a small volume.
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<h2 dir="ltr">What you will find:</h2>
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DNA Clean &amp; Concentrator&trade; (DCC): Efficient spin-column systems (5, 25, 100, 500 preps) created to concentrate low-concentration DNA into ultra-pure, transfection-quality volumes.
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Genomic DNA Clean &amp; Concentrator: Tailored procedures for extracting large templates while maintaining genomic integrity for long-read sequencing uses.
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Select-a-Size DNA Clean &amp; Concentrator&trade;: Tailored systems for the recovery of size-specific fragments, efficiently eliminating adapter dimers and non-target amplicons.
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ZR-96 DNA Cleanup Systems: Scalable 96-well and MagBead configurations designed for high-throughput, automated processes while ensuring reproducibility-validated outcomes.
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<h2>How to Choose a DNA Cleanup Kit</h2>
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Capacity and Input Volume
Zymo DNA Clean &amp; Concentrator kits are available in formats matched to DNA input mass: DCC-5 (&lt;=5 ug input, elute &gt;=6 ul), DCC-25 (&lt;=25 ug input, elute &gt;=25 ul), DCC-100 (&lt;=100 ug input, elute &gt;=25 ul). Choose the format whose maximum capacity exceeds your expected DNA input by at least 2-fold to avoid column overloading, which reduces yield and purity. For genomic DNA preparations from tissue (typically 5-30 ug), DCC-25 or DCC-100 is appropriate.
Minimum Elution Volume and Concentration
For maximum DNA concentration, Zymo DCC-5 elutes in 6 ul - the industry minimum for standard silica columns. If the input volume is 500 ul (1 ng/ul) and the elution is 6 ul, the final concentration reaches approximately 80 ng/ul (assuming 95% recovery), a concentration suitable for transfection, electroporation, and microinjection.
Size Selectivity
Standard DNA cleanup kits retain all DNA &gt;=100 bp. If size selection is also required, use gel extraction (for specific band isolation) or AMPure XP beads (for size-range selection by bead: volume ratio adjustment) rather than a standard cleanup kit.
Post-Phenol-Chloroform Cleanup
For DNA recovered from phenol-chloroform extraction, add 5 volumes of binding buffer and 2 volumes of 100% ethanol to the aqueous phase before column loading. The organic solvent in residual phenol or chloroform does not significantly impair column binding at these dilutions. Multiple column reloads allow processing of large aqueous-phase volumes through a single column.
RNase A Treatment for DNA-Only Preparations
For gDNA preparations that must be RNA-free for optical mapping or certain long-read sequencing library kits, add RNase A (100 ug/ml) to the DNA solution, incubate at 37 degrees C for 10 minutes, then proceed directly to DCC cleanup. The RNase digestion products are removed during the wash steps.
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<h2>Specifications Context</h2>
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Zymo DNA Clean &amp; Concentrator kits use a single-buffer system with Zymo-Spin column technology and recover DNA from PCR, enzymatic reactions, phenol-chloroform extractions, and dilute solutions. DNA is eluted in volumes as small as 6 ul, providing up to 80-fold concentration from 500 ul inputs. The kits remove enzymes, proteins, salts, organic solvents, dyes, unincorporated nucleotides, primers, and other small molecules, delivering DNA ready for restriction digestion, ligation, sequencing, Southern blotting, transfection, and long-read library preparation. DNA cleanup and concentration are standard workflow steps in genomics laboratories at Howard Hughes Medical Institute, Vanderbilt University, and MD Anderson Cancer Center, where removing trace inhibitors from gDNA before library preparation significantly improves NGS library complexity and uniformity.
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Enhance your capabilities&mdash;<a href="https://mbpinc.net/contact-us">reach out to the MBP Inc. team</a> now to obtain a quote for your DNA concentration requirements.

MSDS

Brochure

Protocol

DNA Clean & Concentrator Magbead Kit (100 preps)

D4012 by Zymo Research is a 100-prep magnetic bead DNA cleanup kit compatible with any open liquid handler or magnetic bead mover. Recovers DNA ≥25 bp (up to 150 kb HMW); &gt;90% recovery for ≥100 bp fragments; purity A260/A280 &amp; A260/A230 ≥1.8; elution in 30–50 µl. Suitable for PCR cleanup, NGS library prep, restriction digests, and automated high-throughput purification.

Binding Capacity

Purity

Sample Sources

Size

Manufacturer

Shipping Condition

Storage Condition

DNA Clean & Concentrator-100

D4029 by Zymo Research is the DNA Clean &amp; Concentrator-100 kit for purifying up to 100 µg of DNA from PCR, large restriction digests, or cell-free lysates — 25 preps, &lt;20 min, ≥150 µL elution, 70–95% recovery for 50 bp–10 kb.

Detergent Tolerance

Elution Volume

Equipment

Sample Source

Size Range

Yield

DNA Clean & Concentrator™-100 (25 Preps.)

DNA Clean & Concentrator™-100 (50 Preps.)

DataSheet

DNA Clean & Concentrator-25

D4005 by Zymo Research is the DNA Clean &amp; Concentrator-25, recovering up to 25 µg of DNA from PCR products, restriction digests, and enzymatic reactions in ~2 minutes with 70–90% recovery for fragments 50 bp–10 kb. Samples are eluted in ≥25 µl of water or Tris buffer and are ready for sequencing, ligation, and NGS library preparation.

DNA Clean & Concentrator™-25 (50 Preps.)

DNA Clean & Concentrator™-25 (200 Preps.)

D4033 by Zymo Research — DNA Clean &amp; Concentrator-25, 50 preps. Purifies DNA from PCR and enzymatic reactions in ~2 minutes with up to 25 µg binding capacity and elution in as little as 25 µl. Recovery: 70–90% for 50 bp–10 kb fragments.

DNA Clean & Concentrator-500

DNA Clean &amp; Concentrator-500 D4031 by Zymo Research purifies and concentrates up to 500 µg of DNA from large-scale restriction digests, crude preparations, and high-mass PCR reactions in under 20 minutes, with 70–95% recovery for 50 bp–10 kb fragments. 10 preps per kit.

DNA Clean & Concentrator™-500 (10 Preps.)

DNA Clean & Concentrator™-500 (20 Preps.)

DNA Clean & Concentrator™-5

D4003 by Zymo Research — DNA Clean & Concentrator-5; 50 preps; 2-minute cleanup; ≥6 µL elution; 50 bp–23 kb; ≤5 µg binding capacity; A260/A280 > 1.8; removes enzymes, dNTPs, and buffers from PCR and enzymatic reactions.

DNA Clean & Concentrator™-5 (50 Preps.) (Uncapped)

DNA Clean & Concentrator™-5 Kit (10 Preps.)(Uncapped)

DNA Clean & Concentrator™-5 (200 Preps.)(Uncapped)

DNA Clean & Concentrator™-5 (50 Preps.) (Capped)

DNA Clean & Concentrator™-5 (200 Preps.) (Capped)

DNA Binding Buffer (25 ml)

DNA Binding Buffer (50 ml)

DNA Wash Buffer (Concentrate) (24 ml)

DNA Wash Buffer (Concentrate) (48 ml)

DNA Wash Buffer (Concentrate) (6 ml)

DNA Binding Buffer (100 ml)

D4064-MSDS

D4064-Protocol

Genomic DNA Clean & Concentrator™ Kit-25

D4064 by Zymo Research — Genomic DNA Clean &amp; Concentrator™-25 Kit (25 preps). Purifies and concentrates up to 25 µg high molecular weight DNA (50 bp to 200 kb) from enzymatic reactions and dilute samples in ~5 minutes. Elution ≥ 35 µl; recovery 70–95%; A260/A280 &gt; 1.8. Also available as D4065 (100 preps).

Applicable For

Sample storage

Genomic DNA Clean & Concentrator™ Kit-25 (25 Preps.)

Genomic DNA Clean & Concentrator™ Kit-25 (100 Preps.)

Genomic DNA Clean & Concentrator™-10 Kit

D4010 by Zymo Research is the Genomic DNA Clean &amp; Concentrator™-10, purifying and concentrating gDNA, BAC/PAC DNA, viral DNA, and WGA products up to 200 kb in ~5 minutes with 70–95% recovery. Binding capacity is 10 µg; elution volume ≥10 µl; purity A260/A280 &gt;1.8 and A260/A230 &gt;1.8.

Sample Storage

Genomic DNA Clean & Concentrator™-10 Kit (25 Preps.)

Genomic DNA Clean & Concentrator™-10 Kit (100 Preps.)

D4080-Data Sheet

D4080-MSDS

D4080-1-MSDS

D4080-Protocol

Select-a-Size DNA Clean & Concentrator™ Kit

The D4080 by Zymo Research is a 25-prep Select-a-Size DNA Clean &amp; Concentrator Kit that cleans up and size-selects DNA in a single spin-column workflow, with user-adjustable cutoffs at ≥50, ≥100, ≥150, ≥200, or ≥300 bp and a double-size selection mode for the 50–700 bp window. Binding capacity is up to 3 µg per prep; protocol runs in as little as 7 minutes; A260/280 &gt; 1.8 output.

Select-a-Size DNA Clean & Concentrator™ Kit (25 Preps.)

Select-A-Size Binding Buffer (15 ml)

D4084-Data Sheet

D4084/D4085-MSDS

D4084-4-MSDS

D4084-Protocol

Select-a-Size DNA Clean & Concentrator™ MagBead Kit

D4084 by Zymo Research is the Select-a-Size DNA Clean &amp; Concentrator MagBead Kit — a magnetic bead system for tunable DNA size selection from 150 bp to 800 bp in ≤10 minutes. Supports left-sided, right-sided, and double-sided selection; elution into ≥10 µl; up to 10 µg yield. Compatible with PCR, ligation, NGS library prep, and adapter removal workflows. Available in 10 ml (D4084) and 50 ml (D4085). Store at 4 °C. Made in USA by Zymo Research.

Processing Time

Select-a-Size DNA Clean & Concentrator™ MagBead Kit (10 ml)

Select-a-Size DNA Clean & Concentrator™ MagBead Kit (50 ml)

Select-a-Size MagBeads (10 ml)

Select-a-Size MagBeads (50 ml)

ZR-96 DNA Clean & Concentrator-5 (Deep Well)

D4023 by Zymo Research — ZR-96 DNA Clean & Concentrator-5 deep-well format; 2 × 96 preps; ≥10 µL elution; 50 bp–23 kb; ≤5 µg binding per well; 70–95% recovery; ~15 min for 96 samples.

ZR-96 DNA Clean & Concentrator™-5 (2 x 96 Preps.)

ZR-96 DNA Clean & Concentrator™-5 (4 x 96 Preps.)

ZR-96 DNA Clean-Up Kit

D4017 by Zymo Research is the ZR-96 DNA Clean-Up Kit — a 96-well shallow-well spin-plate system (2 × 96 preps) that purifies DNA from PCR and enzymatic reactions in 20 minutes. Recovery of 70–90% for fragments 75 bp–10 kb, 50–70% for 11–23 kb; elution into ≥30 µl; A260/A280 &gt;1.8; tolerates up to 5% common detergents. Maximum 5 µg per well. Made in USA by Zymo Research.