RNA sequencing

<h2>What are RNA-seq accessories?</h2>
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RNA-seq accessories are the supporting consumables used alongside a core metatranscriptomic library preparation kit, including rRNA depletion reagents that reduce the ribosomal RNA dominating most total RNA extracts, magnetic bead-based reagents for library cleanup and size selection between preparation steps, and quantification or fragment-size quality-control tools used to confirm a library's concentration and integrity before committing it to a sequencing run.
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<h2 dir="ltr">What you will find:</h2>
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Dual-Indexed UDI Primer Sets: High-purity Unique Dual Index (UDI) primers designed to eliminate index hopping and ensure accurate demultiplexing during high-throughput NGS library preparation.
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96-Index Primer Plates: Nuclease-free, high-diversity primer configurations optimized for scalable parallel processing and the detection of rare microbial transcripts with maximum precision.
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<h2>How to choose RNA-seq accessories</h2>
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Match your rRNA depletion reagent to your sample's species complexity
A depletion reagent validated for human and common bacterial species works well for many host-associated samples, while a depletion kit specifically designed for mixed, complex bacterial populations is needed for more diverse environmental or microbiome community samples.
Use magnetic bead cleanup for size selection between library prep steps
Magnetic bead-based cleanup reagents remove unwanted small fragments and reaction components between enzymatic steps in library preparation, and the bead-to-sample ratio used can be adjusted to select for a specific fragment size range appropriate to your sequencing platform.
Confirm library quantification method matches your downstream platform requirements
A fluorometric or fragment-analysis-based quantification method provides the library concentration data needed to pool multiple samples accurately for multiplexed sequencing, and using a method appropriate to your specific library type avoids over- or under-loading the sequencer.
Check fragment size distribution before sequencing to catch problems early
Running a quality-control check of library fragment size distribution, using an instrument like a Bioanalyzer or similar fragment analyzer, helps catch a library prep problem, such as adapter dimer contamination, before committing the sample to a full sequencing run.
Plan for accessory compatibility with degraded or low-input RNA samples
Some accessories and protocols accommodate degraded RNA with low RNA integrity number (RIN) scores, such as RNA from challenging sample types, though performance may be somewhat reduced compared with high-quality, intact RNA input.
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<h2>Specifications context</h2>
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A streamlined rRNA depletion accessory can effectively deplete multiple rRNA species in a single-tube reaction, reducing the hands-on accessory steps needed compared with older multi-step depletion protocols, while still requiring downstream library cleanup and quantification accessories regardless of which depletion method is used. As of 2026, fragment analyzer and similar QC instrumentation remain a standard accessory-stage check before sequencing, helping confirm library quality issues are caught and addressed prior to committing limited sequencing capacity to a problematic sample.
<a href="https://mbpinc.net/contact-us">Contact the helpful team at MBP Inc.</a> today to request a quote for your RNA-Seq accessory needs.

RNA-seq accessories

DataSheet

Protocol

Zymo-Seq UDI Primer Plate (Indexes 1-96)

D3096 by Zymo Research provides 96 unique dual-index (UDI) primer pairs — indexes 1–96 — in a pre-formatted 96-well plate for multiplexed Illumina® NGS library preparation. Each well delivers 10 µL at 5 µM total primer concentration, compatible with TruSeq® adapter-based kits including Zymo-Seq RiboFree® and SwitchFree™. Store at -80°C.

Manufacturer

Shipping Condition

Storage Condition

MSDS

Zymo-Seq UDI Primer Set (Indexes 1-12)

D3008 by Zymo Research is the Zymo-Seq UDI Primer Set (Indexes 1–12)—12 unique dual-index primer pairs, each pre-mixed at 5 µM total (2.5 µM i5 + 2.5 µM i7) in individual 1.5 mL tubes. Compatible with the Zymo-Seq RiboFree® Total RNA and SwitchFree 3′ mRNA library kits and any Illumina TruSeq® adapter-based library kit, enabling unambiguous multiplexing of up to 12 samples on any Illumina sequencer.

United States

Texas

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