dNTPs (Deoxynucleotide Triphosphates)

<h2>What are methylated dNTPs?</h2>
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Methylated dNTPs are base-modified deoxynucleotide analogs, the most important being 5-methyl-dCTP (2'-deoxy-5-methylcytidine 5'-triphosphate), in which the cytosine base carries a methyl group at the carbon-5 position. This is the same position methylated in naturally occurring genomic 5-methylcytosine, the primary DNA methylation mark studied in epigenetics research. Substituting 5-methyl-dCTP for standard dCTP in a PCR reaction or in vitro synthesis produces a defined, fully cytosine-methylated DNA template from a known sequence.
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<h2 dir="ltr">What you will find:</h2>
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5-Methyl dCTP (10 mM) (100 &micro;l): for high-purity enzymatic incorporation into methylated DNA strands.
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5-Methylcytosine dNTP Mix (10 mM) (250 &micro;l): pre-validated nucleotide blends for controlled epigenetic assay workflows.
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<h2>How to use methylated dNTPs</h2>
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Substitute 5-methyl-dCTP for dCTP in PCR to synthesize defined methylated DNA
A PCR reaction using 5-methyl-dCTP in place of standard dCTP produces a fully methylated amplicon in which every cytosine carries the 5-methyl modification, providing a positive control with a known and consistent methylation status distinct from the partial, variable methylation of a genomic DNA control.
Use methylated DNA as a positive control for bisulfite or enzymatic conversion workflows
Running 5-methyl-dCTP-synthesized fully methylated DNA through your bisulfite or enzymatic conversion protocol confirms the method correctly preserves methylated cytosines while converting unmethylated ones, catching conversion efficiency problems at the control stage before they affect interpretation of real samples.
Test methylation-sensitive restriction enzymes using 5-methyl-dCTP-produced substrates
Some restriction enzymes cut methylated sequences while others are inhibited by methylation at their recognition site, and a PCR-synthesized, fully methylated substrate of known sequence provides a clean, defined test of whether a specific enzyme is methylation-sensitive or methylation-dependent.
Use as an in vitro substrate for TET enzyme oxidation studies
Since 5-methylcytosine is the starting substrate for TET family enzyme oxidation into 5-hydroxymethylcytosine and further products, fully methylated DNA synthesized with 5-methyl-dCTP provides a defined, homogeneous substrate for in vitro TET enzyme activity assays.
Store methylated dNTPs appropriately to maintain activity
Methylated dNTP analogs are generally stored at -20&deg;C, similar to standard dNTPs, with short-term exposure to ambient temperature acceptable but prolonged room-temperature storage avoided to prevent hydrolysis of the phosphate groups.
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<h2>Specifications context</h2>
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5-methyl-dCTP is typically supplied as a 10 mM solution at pH 7.0 and can be diluted using sterile distilled water or TE buffer, and it can be used in PCR, RT-PCR, and other DNA synthesis applications at the same concentration as standard dCTP in a reaction mix, with 5-methylated DNA probes made this way serving as methylation reference fragments or as pull-down substrates for 5-hydroxymethylcytosine-binding proteins. As of 2026, 5-methyl-dCTP-synthesized control DNA remains a standard tool in epigenetics research labs specifically because it provides a modification-defined substrate without relying on the variable or partially characterized methylation patterns of genomic positive controls.
<a href="https://mbpinc.net/contact-us">Contact the expert team at MBP</a> and get high-quality methylated (dNTPs) for your lab.

Methylated

DataSheet

5-Methyl dCTP (10 mM) (100µl)

Zymo Research D1035: 5-Methyl dCTP at 10 mM, 100 µl (1 µmol). &gt;99% purity by HPLC; free of DNase, RNase, and phosphatase. Compatible with ZymoTaq, QuestTaq, and standard thermostable polymerases. Store at –20°C.

Applicable For 

Concentration

Sample Storage

Manufacturer

Shipping Condition

Storage Condition

5-Methylcytosine dNTP Mix (10 mM) (250 µl)

5-Methylcytosine dNTP Mix (ZYM-D1030 / Zymo Research D1030) is a ready-to-use 10 mM dNTP mix with 5-methyl-dCTP replacing dCTP for PCR-based generation of fully 5-methylcytosine-substituted DNA products, resistant to methylation-sensitive restriction enzymes. 250 µl; store at −20°C.

<h2>What are epigenetics dNTPs?</h2>
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Epigenetics dNTPs includes both standard deoxynucleotide triphosphate sets used across many molecular biology applications and modified nucleotide analogs relevant specifically to epigenetic research, with the most important being 5-methyl-dCTP, a base-modified analog in which the cytosine base carries a methyl group at the 5-carbon position. 5-methyl-dCTP can be incorporated into DNA via PCR or in vitro synthesis in place of standard dCTP, producing a defined, fully methylated cytosine-substituted DNA molecule useful as a positive control for methylation detection assays or as a substrate for studying methylation-related enzyme activity.
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<h2 dir="ltr">What you will find:</h2>
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<ul>
<li><a href="https://mbpinc.net/reagents/epigenetics/dntps-deoxynucleotide-triphosphates/methylated">Methylated: </a>dNTP mixes used in methylation-sensitive DNA synthesis workflows.&nbsp;Methylated nucleotide analogs for DNA methylation studies and epigenetics research.&nbsp;Standard individual dNTPs (dATP, dCTP, dGTP, dTTP) for PCR analysis and routine DNA synthesis.</li>
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<h2>How to choose epigenetics dNTPs</h2>
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Use 5-methyl-dCTP to build defined, fully methylated control DNA
Substituting 5-methyl-dCTP for standard dCTP in a PCR reaction produces a fully methylated cytosine-substituted amplicon, providing a positive control whose methylation status is defined and known, unlike a positive control derived from genomic DNA with variable, unknown methylation patterns.
Confirm that your methylation detection assay is validated with a defined methylated control
Running a fully methylated positive control through a bisulfite conversion or enzymatic conversion workflow confirms the detection method is correctly interpreting methylated cytosines, helping catch conversion efficiency problems before they affect interpretation of real sample results.
Use methylated dNTP-synthesized DNA to study methylation-sensitive restriction digestion
Some restriction enzymes specifically cleave methylated sequences while others are inhibited by methylation, and a fully methylated DNA produced with 5-methyl-dCTP is a useful substrate for confirming whether a specific enzyme's activity is methylation-sensitive.
Be aware that 5-methyl-dCTP is also believed to be the starting substrate for TET enzyme oxidation
5-methylcytosine is believed to be the substrate for TET family enzymes that produce 5-hydroxymethylcytosine and further oxidation products, making 5-methyl-dCTP-synthesized DNA a potential substrate for in vitro TET enzyme activity studies.
Confirm nucleotide concentration and compatibility with your synthesis conditions
Standard dNTPs and modified analogs like 5-methyl-dCTP are typically supplied as concentrated solutions requiring dilution, and confirming appropriate concentration and buffer compatibility before use ensures consistent, reproducible incorporation into your target DNA.
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<h2>Specifications context</h2>
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5-methyl-dCTP is typically supplied as a 10 mM solution and offers the ability to enzymatically make a defined, fully methylated, cytosine-substituted DNA by PCR or in vitro synthesis, a property that makes it a valuable resource for a variety of biochemical and cellular applications in epigenetics research. As of 2026, methylated DNA probes made with 5-methyl-dCTP continue to serve as methylation reference fragments and as pull-down substrates for 5-hydroxymethylcytosine-binding proteins from cellular lysates, extending their use beyond simple positive controls.
<a href="https://mbpinc.net/contact-us">Contact the expert team at Molecular Biology Products Inc. (MBP)</a> to find high-integrity dNTPs tailor-made for your research applications.

MSDS

Protocol

dATP (100 mM) (250 µl)

D1005 by Molecular Biology Products (MBP Inc.) is a 100 mM dATP (deoxyadenosine triphosphate) solution supplied in a 250 µL volume. Ready for direct use in PCR, RT-PCR, nucleotide mix preparation, library preparation, and other molecular biology applications requiring individual dNTP components at a defined molar concentration.

Storage

dCTP (100 mM) (250 µl)

D1020 by Zymo Research is 100 mM dCTP solution (250 µl, 25 µmol) with >99% trisphosphate purity by HPLC, free of nuclease and phosphatase contaminants. Use 0.5 µl per 50 µl PCR reaction for a 0.25 mM final concentration; compatible with ZymoTaq™ and other standard DNA polymerases. Store at -20°C (12-month guarantee) or -80°C for extended periods. MBP is a registered vendor for Howard Hughes Medical Institute, Vanderbilt University, MD Anderson Cancer Center, Eurofins, and many other institutes across the USA.

dGTP (100 mM) (250 µl)

C2011-2 by Zymo Research — 2-pack of Air Permeable Sealing Covers for 96-well microcentrifuge spin-column and collection plates. Allows gas exchange while preventing cross-contamination and reducing evaporation during incubation steps. Compatible with Zymo-Spin I-96-XL Plates (C2010). Also available in 4-pack (C2011-4) and 8-pack (C2011-8).

dTTP (100 mM) (250 µl)

D1010 by MBP is a 100 mM dTTP (deoxythymidine triphosphate) solution supplied in a 250 µl volume, formulated for direct use in PCR, RT-PCR, nucleotide mix preparation, and library construction protocols. Store at -20°C.